KR102162917B1 - Cosmetic composition comprising complex extracts of Artemisia absinthium, Belamcanda chinensis, Sedum sarmentosum, Solidago virgaurea and Actinidia polygama - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a complex extract of Artemisia absinthium, Iris domestica, Sedum sarmentosum, Solidago virgaurea, and Actinidia polygama. The complex extract as an active component consists of natural extracts, thereby having less side effects and skin irritation, and thus exhibiting excellent effects of alleviating atopic skin and acne, and relieving skin irritation.

Description

Cosmetic composition comprising complex extracts of Artemisia absinthium, Belamcanda chinensis, Sedum sarmentosum, Solidago virgaurea and Actinidia polygama}

The present invention relates to a cosmetic composition containing a complex extract of bitter wormwood, panworm, sedum, seaweed odor, and dogwood, and the complex extract as an active ingredient consists of a natural extract and has less side effects and skin irritation, and has excellent atopic skin improvement, acne improvement and skin It has an irritation relief effect.

The skin is an important organ that protects the body from external stimuli, and the surface of the skin consists of a structure in which the sebaceous film secreted from the sebum and sweat glands covers the stratum corneum, always replenishing insufficient moisture and maintaining a shiny and healthy state. In addition, the skin protects the skin from ultraviolet rays and plays an important role in the body, such as controlling the temperature in the body. Compared to other organs, the skin is an organ that is regularly extinct and produced. It functions to physically protect and prevent moisture loss in the body. Ideally, when the moisture content of the stratum corneum is 20%, when moisture is lost, the keratin is removed and exposed to the outside, resulting in irritation-induced inflammation and skin disease. Dry skin weakens the immune function, making it easier to induce allergies such as skin erythema, itching, and itching as well as atopic dermatitis. Therefore, it is important to prevent moisture loss in the skin.

Cosmetics used for protecting skin, strengthening the barrier and enhancing skin are mixed with various ingredients. Solubilizers, emulsifiers, preservatives, fragrances, sunscreens, and other functional active ingredients that are less related to the purpose of skin protection It is known to cause irritation, inflammation, allergies, erythema, swelling, and itching. Therefore, interest in substances that relieve irritation of cosmetics is increasing. Therefore, various methods have been attempted to relieve skin irritation. For example, attempts have been made to remove substances that cause skin irritation. However, since the functional raw material itself often acts as an irritant, there is a limitation in such research, and it is required to use a material having an effect of reducing side effects and alleviating irritation while maintaining the effect of the functional raw material. As an example, Korean Patent Application Publication No. 2009-0073632 discloses'a cosmetic material for alleviating skin irritation for the purpose of alleviating irritation and anti-inflammatory effects containing Yedeok tree extract and licorice extract', and Korean Patent No. 1064570 discloses' A composition for relieving skin irritation due to erythema, itching, or itching containing a mixed extract of Gosam, Cinnamon, Sambaekcho and Tangja as active ingredients is disclosed.

The present inventors investigated various natural products in order to develop a cosmetic composition for improving atopic skin and acne that has little side effects and skin irritation and is effective. As a result, it was confirmed that the complex extracts of bitter mugwort, beom fan, sedum, seaweed, and dogtail have less skin irritation and excellent atopic skin, acne improvement, and skin irritation alleviation effect, thereby completing the present invention.

Korean Registered Patent No. 2009-0055243 (2009.06.02) Republic of Korea Patent Registration No. 2014-0069470 (2014.06.10.)

An object of the present invention is to provide a cosmetic composition that contains bitter wormwood, beom fan, sedum, seaweed and dogwood complex extracts as active ingredients, which has less skin irritation and exhibits excellent atopic skin improvement, acne improvement, and skin irritation relief effects.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition containing bitter wormwood, beom fan, sedum, seaweed and dogtail extract as an active ingredient.

The complex extract is made by mixing the extracts of bitter mugwort, beom fan, sedum sprout, seaweed, and dogtail in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2, respectively.

More preferably, the extract of the bitter wormwood, panchira, sedum, seaweed and dogtail is at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol After extraction with a hydrolase, more preferably, it is prepared by hydrolysis with β-glucosidase.

The complex extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that it is for alleviating irritation.

The cosmetic composition is characterized in that it is for improving acne.

The cosmetic composition is characterized in that it is for improving atopic skin.

The combined extract of bitter wormwood, beetroot, sedum, seaweed and dogtail according to the present invention is composed of natural extracts, has little side effects and skin irritation, and exhibits excellent acne improvement, atopic skin improvement, and skin irritation relief.

Hereinafter, the present invention will be described in detail.

In the present invention, five kinds of natural extracts, including dogtail, specifically, bitter wormwood, beetroot, sedum, seaweed and dogtail, have less skin irritation, and excellent skin irritation relief, atopic skin improvement activity and acne It is an invention characterized in that it exhibits an improvement activity.

Artemisia absinthium has about 300 species all over the world, and is mainly distributed in subtropical and temperate regions of Asia, Europe and North America. About 186 species and 44 varieties are found in China, distributed throughout. In this genus, about 23 species are used as medicines. Bitter mugwort is distributed not only in the midwestern and northern temperate regions of China, but also in Europe, North Africa, Canada, the eastern region of North America, and the northern region of Tianshan in the Xinjiang of China. Bitter mugwort and its extracts have traditionally been used as repellents and antiperspirants, and the leaves and inflorescences of bitter mugwort have also been used as fragrances, sedatives, and spices. Absinthe, which mainly contains bitter wormwood extract, remained a popular liquor until the early 20th century. This species is the official origin plant species for the medicinal herb Absinthii Herba and is listed in the European Pharmacopoeia (5th edition) and the British Pharmacopoeia (2002). This medicinal herb is mainly produced in Eastern Europe. Bitter mugwort mainly contains sesquiterpene lactone, essential oils and flavonoids. The European Pharmacopoeia and the British Pharmacopoeia stipulate that the essential oil content must be 2mL/kg or more for quality control of medicinal substances. According to pharmacological studies, it was confirmed that bitter worm has antiparasitic, hepatoprotective, ear infection, antibacterial, anti-inflammatory and antioxidant effects. According to folk remedies, bitter mugwort has the effect of geonwi and yidam, and in oriental medicine, it is said that cheongyeol dehumidification, insecticide, and geonwi. In the present invention, leaves, stems and/or outposts are used.

Belamcanda chinensis grows in mountains and in the sea. It is 50-100cm in height. The rhizome is shortly stretched to the side, and the stem stands upright, and branches are cut from the top. Leaves are alternate, sword-shaped, flat to the left and right, and lined up in 2 rows. The color is a little white on a green background, and it wraps around the stem. The leaf length is 30-50cm and the butterfly is 2-4cm. Flowers bloom in July-August, 5-6cm in diameter, spread horizontally, and have dark spots on a yellowish red background. The end of the branch is split 1 or 2 times, and several flowers hang in one place, and there are 4-5 bracts at the bottom. There are 6 pieces of flower skin and it is oval. There are 3 stamens and the ovary is inferior. The style stands upright and splits into three. Fruits are capsules, in the shape of an egg upside down, 3cm long, and ripen in September to October. The seeds are ball-shaped, black, and shiny. It is cultivated for ornamental purposes and the rootstock is used as medicine. It is distributed in Korea, Japan, and China. In the present invention, leaves, stems, roots and/or outposts are used.

Sedum sarmentosum grows in the mountains. The stem extends to the side and roots emerge from each node. The stalk is upright and the height is about 15cm. The leaves usually turn 3 each, have no petiole, and are long oval or lanceolate. Both ends of the leaves are sharp and the edges are flat. The flower is yellow, blooms in August-September, forms inflorescences at the end of the stem, and is 6-10mm in diameter. The 5 petals are lanceolate, with a sharp tip and longer than the calyx. There are 5 calyx pieces, and the end is blunt in the shape of an oval lance. There are 10 stamens, about the same length as the petals. The fruit is Goldolaceae (利咨果), and there are 5 simpi (心皮). If you cut the stem and put it in the ground, it will grow well. Young stems and leaves are made with kimchi and have a flavor. Light hansoon is made with herbs. It is distributed throughout Korea, Japan, and China. In the present invention, leaves, stems, roots and/or outposts are used.

Seaweed ( Solidago virgaurea ) is also known as pork sprouts. It grows in the sunny meadows of mountains and fields. The stem stands straight, the branches are split at the upper part, dark purple, with fine hairs, and the height is 30~85cm. When flowers bloom, leaves from the roots disappear. Leaves from stem have petioles with wings, oval-shaped, oval-shaped long oval or long oval-shaped lanceolate, pointed ends, some hairs on the surface, and serrated edges. Leaves become smaller and narrower as it goes above the stem, and petioles disappear. Flowers bloom yellow in July-October, and 3 to 5 headflowers run in corymb inflorescences, forming large spikes. The head flower is 1.2-1.4cm in diameter, and there are female snowflakes arranged in one row at the edge, and there are several bisexual ornamental flowers in the middle. The gun is shaped like a barrel, and the gun pieces are arranged in 4 rows. Fruit is achene, cylindrical, and the length of the tubular hair is 3.5mm. Eat young shoots with herbs. In oriental medicine, the plant is used as a medicinal material called Iljihwanghwa (一枝黃花), which is effective for headaches, sore throat, and tonsillitis caused by colds, and is also used for jaundice and bruises, and juice is applied at the beginning of the boil. It is distributed in Korea and Japan. In the present invention, leaves, stems, roots and/or outposts are used.

Actinidia polygama is a deciduous vine plant of the Asteraceae family, and is also known as the horsetail tree. It grows under trees in deep mountains or in valleys. It is about 5m long and the inside of the stem is white. The twigs have light brown hairs when they are young, but rarely have strong hairs like thorns. Leaves are alternate, membranous, broad oval-shaped or oval, and the ends become sharper. The upper half of the front side of the leaf may turn white. There are brown hairs on the veins and fine teeth. In June-July, 3-10 white flowers with a diameter of 1.5cm hang on the axilla of the upper part of the branch. There are 5 calyxes and petals, each with a scent. The fruit is a berry, long oval, ripens yellow in September to October and hangs down. You can eat the fruit, but it tastes like a tongue and is not sweet. Herbal medicine Mokcheonryoja is a lump of insects caused by parasitic insects on the fruit and dried with the fruit. It is prescribed for stroke, facial nerve palsy, colic, and low back pain due to its efficacy in geopung and ventilation. The branches and leaves are called Mokcheonryo and the roots are called Mokcheonryoeun. It is distributed in Korea (all regions excluding Chungbuk), Japan, Sakhalin Island, and Kuril Islands. In the present invention, fruit is used.

According to one embodiment of the present invention, as an active ingredient, bitter mugwort, beom fan, sedum sprouts, seaweed and dogwood complex extract are prepared by the following method.

First, extracts are prepared by extracting bitter wormwood, beetroot, sedum sprouts, seaweed odor, and red seaweed with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol. do. Ultrasonic extraction can be performed in parallel.

Each extract thus prepared is mixed to prepare a complex extract. At this time, the complex extract is made by mixing the extracts of bitter mugwort, beom fan, sedum, seaweed, and dogtail in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2, respectively, and 2: 2 It is more preferable because it shows the most excellent activity when it is mixed in a weight ratio of 1: 1: 2: 1.

At this time, it is more preferable that the extracts of bitter wormwood, beompanchae, sedum, seaweed and dogtail, which constitute the complex extract, are prepared by hydrolyzing with a hydrolase, preferably β-glucosidase, respectively, after the solvent extraction.

The bitter wormwood, beom fan, sedum sprout, seaweed, and dogwood complex extract prepared in this way exhibited excellent atopic skin improvement, acne improvement, and skin irritation relief.

The complex extract as an active ingredient is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

In the present invention, the complex extract may be prepared by first mixing bitter mugwort, beom fan, sedum sprouts, seaweed odor and dogtail to prepare a mixture and then extracting it.

The cosmetic composition of the present invention can be prepared in any conventionally prepared formulation, such as lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, conditioner, hair These include conditioner, hair gel, foundation, lipstick, mascara, and makeup base.

[Example]

Although the present invention is described in detail through preparation examples, examples and test examples, the present invention is not limited by these examples.

Preparation Example 1: Preparation of bitter mugwort extract

After washing the outpost of bitter mugwort with purified water, dry it, add 5 times the weight of 70% hydrated ethanol as an extraction solvent, and heat it at 80~100℃ in an extractor equipped with a cooling condenser (Cosmos-660, Kyungseo Machine) for a total of 3 for 2 hours. Extracted twice.

After extraction by the above method, it was allowed to stand at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. Then, after concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), inlet temperature 180℃ using a spray dryer (B-290, BUCHI), and an aspirator Drying under conditions of 100% efficiency (35cm3/hour), 25% pump efficiency (7.5ml/min), Nozzle Cleaner 4 and Rotameter: 30㎜(357liter/hour) To obtain 10 g of the title extract powder.

Preparation Example 2: Preparation of Beomwicha Extract

The roots of Beomchia were extracted in the same manner as in Preparation Example 1 to prepare a Beomchia extract.

Preparation Example 3: Preparation of sedum extract

The sedum outpost was extracted in the same manner as in Preparation Example 1 to prepare a sedum extract.

Preparation Example 4: Preparation of seaweed extract

The seaweed extract was prepared by extracting the seaweed extract in the same manner as in Preparation Example 1.

Preparation Example 5: Preparation of dogtail extract

Dogtail fruit was extracted in the same manner as in Preparation Example 1 to prepare a dogtail extract.

Preparation Example 6: Preparation of Bitter Mugwort Enzyme Extract

Bitter mugwort outpost is washed with purified water, dried, and swelled for 48 hours by immersing in 70% water-containing ethanol equivalent to 5 times the weight as an extraction solvent, and then swelling for 48 hours at 30℃ for 15 minutes at 25KHz using an ultrasonic device. It was treated under repeated conditions of 3 times. After extraction by the above method, it was allowed to stand at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. Then, after concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), inlet temperature 180℃ using a spray dryer (B-290, BUCHI), and an aspirator Drying under conditions of 100% efficiency (35cm3/hour), 25% pump efficiency (7.5ml/min), Nozzle Cleaner 4 and Rotameter: 30㎜(357liter/hour) To obtain 10 g of the title extract powder.

10 g of the extract powder of the bitter mugwort extract was added to 500 ml of acetate buffer (pH 4.0) and 25 ml of β-glucosidase enzyme and reacted at 55° C. at 100 rpm for 4 hours. Next, after heating to 90° C. for 5 minutes to complete the enzyme reaction, the reaction product was concentrated in vacuo. The concentrate was dried at 60° C. for 30 hours. After extraction by the above method, it was allowed to stand at room temperature for 3 days, and the precipitate was filtered through a 300 mesh filter paper, and the precipitate was filtered twice through an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. Then, after concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), inlet temperature 180℃ using a spray dryer (B-290, BUCHI), and an aspirator Drying under conditions of 100% efficiency (35cm3/hour), pump efficiency 25% (7.5ml/min), Nozzle Cleaner 4 and Rotameter: 30㎜ (357liter/hour) To obtain a bitter mugwort enzyme extract.

Preparation Example 7: Preparation of Enzyme Extract of Pan-Fried Vegetables

The roots of Beomchia were extracted in the same manner as in Preparation Example 6 to prepare an enzyme extract from Beomchia.

Preparation Example 8: Preparation of sedum enzyme extract

Sedum outpost was extracted in the same manner as in Preparation Example 6 to prepare sedum enzyme extract.

Preparation Example 9: Preparation of enzyme extract of seaweed odor

Seaweed outpost was extracted in the same manner as in Preparation Example 6 to prepare a seaweed extract enzyme extract.

Preparation Example 10: Preparation of Enzyme Extract of Dogwood

Dogtail fruit was extracted in the same manner as in Preparation Example 6 to prepare a dogtail enzyme extract.

Examples 1-5: Preparation of complex extract

The extracts of Preparation Examples 6 to 10 were mixed in the weight ratio shown in Table 1 to prepare complex extracts of the same weight.

(Weight ratio) Mixing ratio Example 1 1:1:1:1:1 Example 2 2:2:2:1:1 Example 3 1:1:1:2:2 Example 4 1:2:2:1:2 Example 5 2:2:1:2:1

Test Example 1: Confirmation of cytotoxicity

In order to check the cytotoxicity, fibroblasts were inoculated in IMDM medium to which 10% FBS was added at a cell concentration of 5×10 ⁵ and cultured in a 37° C. 5% CO ₂ incubator for 24 hours. After cultivation, the medium was removed, and the extract prepared in Preparation Examples and Examples was diluted in dimethyl sulfoxide to a sample concentration of 50, 100, 500, and 1000 µg/ml, and the prepared diluted solution was treated and cultured for 24 hours. 100 ml of MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazoliumboromide, Sigma, USA) solution was added to each well (3 mg/ml) and then incubated for 4 hours. Thereafter, the supernatant was removed, 150 µl of dimethyl sulfoxide was added, and the resulting formazan was dissolved by shaking for 30 minutes, and the absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the following equation, and the results are shown in Table 2 below.

Cell viability (%) = absorbance of the sample added group/absorbance of the control group × 100

division Cell viability (%) 0㎍/㎖ 50㎍/㎖ 100㎍/㎖ 500㎍/㎖ 1000㎍/㎖ Manufacturing Example 1 100 100 100 100 96.7 Manufacturing Example 2 100 100 100 100 96.9 Manufacturing Example 3 100 100 100 100 97.5 Manufacturing Example 4 100 100 100 100 96.5 Manufacturing Example 5 100 100 100 100 97.6 Manufacturing Example 6 100 100 100 100 96.4 Manufacturing Example 7 100 100 100 100 98.1 Manufacturing Example 8 100 100 100 100 97.9 Manufacturing Example 9 100 100 100 100 96.4 Manufacturing Example 10 100 100 100 100 96.6 Example 1 100 100 100 100 97.9 Example 2 100 100 100 100 98.9 Example 3 100 100 100 100 98.5 Example 4 100 100 100 100 98.8 Example 5 100 100 100 100 99.1

As confirmed in Table 2 above, it was confirmed that both the Preparation Example and the Example samples did not affect cytotoxicity, and there was no problem with safety.

Test Example 2: Confirmation of inhibitory effect on expression of inflammatory mediators

RAW 264.7 cells were adjusted to 5×10 ⁵ cells/well using DMEM medium added with 10% FBS, and then inoculated into 12 well plates and cultured for 18 hours. After incubation, the starvation was maintained for 12 hours, and then 10 ng/mL of LPS and the sample were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of TNF-α expression was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The TNF-α expression inhibition rate was calculated by the following formula, and the results are shown in Table 3 below. As a control, Dexamethasone was used.

TNF-α expression inhibition rate (%) = (sample TNF-α expression amount / control group TNF-α expression amount) × 100

Sample name Treatment concentration (w/v%) TNF-a expression inhibition rate (%) Manufacturing Example 1 0.1 50.6 Manufacturing Example 2 0.1 51.2 Manufacturing Example 3 0.1 53.2 Manufacturing Example 4 0.1 49.6 Manufacturing Example 5 0.1 48.5 Manufacturing Example 6 0.1 35.6 Manufacturing Example 7 0.1 54.5 Manufacturing Example 8 0.1 52.3 Manufacturing Example 9 0.1 51.5 Manufacturing Example 10 0.1 52.5 Example 1 0.1 65.4 Example 2 0.1 66.1 Example 3 0.1 68.1 Example 4 0.1 69.7 Example 5 0.1 78.8 Dexamethasone 0.1 81.8

As shown in Table 3, it was possible to confirm the TNF-α inhibitory effect in all of the samples tested at a concentration of 0.1 (w/v)%. The complex extracts of Examples 1 to 5 of the present invention exhibited superior TNF-α inhibitory effect compared to the extracts of Preparation Examples 1 to 10, and in particular, the complex extract of Example 5 exhibited the most excellent effect.

Test Example 3: Measurement of triglycerides

Seeding 1×10 ⁶ sebocytes in a 100mm culture dish and adding 10~9M substance P to show 70% confluency, remove the culture medium and test drug. Was processed. After 5 days, the medium was removed, washed three times with physiological saline (PBS), and then collected by scraping with a scraper into PBS, followed by centrifugation at 1200 rpm for 5 minutes to collect the cells and remove the PBS. The composition of the lipid extract was made of 0.9% NaCl + 1% triton X 100.

The lipid extract was added, vortexed to homogenize, and then centrifuged at 13,000 rpm for 15 minutes, and only the supernatant was collected.

Afterwards, triglycerides were measured by the enzyme method according to the kit manufacturer (ASAN co. Seoul, Korea), and the protein was quantified by the Bradford method, and the amount of triglycerides per unit concentration protein was corrected. The results are summarized in Table 4 below.

division Amount of triglycerides Manufacturing Example 1 1.1864 Manufacturing Example 2 1.0685 Manufacturing Example 3 1.2974 Manufacturing Example 4 1.2642 Manufacturing Example 5 1.2345 Manufacturing Example 6 1.3421 Manufacturing Example 7 1.3967 Manufacturing Example 8 1.2745 Manufacturing Example 9 1.0975 Manufacturing Example 10 1.1142 Example 1 0.8513 Example 2 0.8916 Example 3 0.8579 Example 4 0.8346 Example 5 0.8113

From Table 4 above, it was confirmed that the complex extract of the Example of the present invention significantly reduced sebum production compared to the extracts of the Preparation Example. Among them, it was found that the sebum production was most significantly reduced in Example 5, followed by Example 4 and Example 1 in that order.

Test Example 4: Measurement of sebum production inhibition

The sebaceous gland cells were cultured in a medium to which 10% FBS was added to DMEM/F-12 (1:1), and 2 X 10 ³ cells were attached to a 96 well plate and cultured for 2 days. Substance P and Preparation Examples 1 to 10 and Examples 1 to 5 were diluted with 1,3-butylene glycol, administered at a concentration of 0.1% by weight, and further cultured for 3 days. After washing with PBS, intracellular lipids were stained with nile red. Fluorescence was measured using a multifunctional enzyme immunoassay device, and the amount of sebum produced in sebaceous gland cells was measured using an excitation 485nm, emission 565nm filter. Table 5 below is a result showing the fluorescence intensity ratio using the untreated group as 1.

sample Fluorescence intensity ratio (control=1) Substance P (10 ^-9 M) 1.66 Substance P (10 ^-9 M) + Preparation Example 1 1.60 Substance P (10 ^-9 M) + Preparation 2 1.58 Substance P (10 ^-9 M) + Preparation 3 1.59 Substance P (10 ^-9 M) + Preparation 4 1.56 Substance P (10 ^-9 M) + Preparation 5 1.53 Substance P (10 ^-9 M) + Preparation 6 1.54 Substance P (10 ^-9 M) + Preparation 7 1.54 Substance P (10 ^-9 M) + Preparation 8 1.60 Substance P (10 ^-9 M) + Preparation 9 1.52 Substance P (10 ^-9 M) + Preparation 10 1.55 Substance P (10 ^-9 M)+ Example 1 1.25 Substance P (10 ^-9 M)+ Example 2 1.34 Substance P (10 ^-9 M)+ Example 3 1.32 Substance P (10 ^-9 M)+ Example 4 1.30 Substance P (10 ^-9 M)+ Example 5 1.17 Negative control (control) 1.00

As shown in Table 5, when substance P was added, lipid production was significantly increased, and when Preparation Examples 1 to 10 and Examples 1 to 5 were administered together, it was confirmed that intracellular sebum production decreased. Therefore, it can be seen that Preparation Examples 1 to 10 and Examples 1 to 5 reduce sebum production by the substance P. In the complex extracts of Examples 1 to 5, the sebum production reduction effect was more excellent.

Test Example 5: Antimicrobial activity test against Propionibacterium acnes

For the extracts of Preparation Examples and Examples, antimicrobial activity against Propionibacterium acnes was measured using the Agar diffusion method. The Propionibacterium acnes 3314 strain used in this test was sold and used at the Biological Resource Center of the Korea Research Institute of Bioscience and Biotechnology. Brain Heart Infusion agar was sterilized on the plate, and 25 ml per plate were dispensed. Strain smear 100 µl of each strain was smeared according to the number of bacteria to 10 ⁵ . After adjusting the samples of Preparation Examples 1 to 10 and Examples 1 to 5 dissolved in 10% Ethanol to concentrations of 0.1%, 0.5%, and 1.0%, 50 µl each disc (paper disk (10 mm), Advantec Filter Paper, Toyo Roshi Kaisha) Ltd, Japan). Salicylic acid was used as a positive control, and Salicylic acid dissolved in 10% Ethanol was adjusted to 0% and 1.0% concentrations and loaded onto discs (paper disk (10mm), Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) by 50 µl. I did. The loaded disc was picked up with sterilized tweezers and planted on the plate with bacteria. The plate was placed in an oxygen-blocking anaerobic jar and cultured at 37°C, and the size (in mm) of the circular growth-stopping ring formed around the paper disk was measured, and all tests were independently repeated three times to obtain an average value. The results are shown in Table 6 below.

division Diameter(mm) 0% 0.1% 0.5% 1.0% Manufacturing Example 1 0 0.43 0.75 0.98 Manufacturing Example 2 0 0.39 0.73 0.97 Manufacturing Example 3 0 0.41 0.72 0.97 Manufacturing Example 4 0 0.48 0.71 0.91 Manufacturing Example 5 0 0.4 0.76 0.95 Manufacturing Example 6 0 0.37 0.74 0.96 Manufacturing Example 7 0 0.45 0.69 0.94 Manufacturing Example 8 0 0.43 0.79 0.92 Manufacturing Example 9 0 0.42 0.79 0.98 Manufacturing Example 10 0 0.43 0.75 1.02 Example 1 0 1.52 2.15 4.55 Example 2 0 1.65 2.25 4.65 Example 3 0 2.15 2.34 5.12 Example 4 0 1.95 2.45 5.63 Example 5 0 3.25 6.58 7.57 Positive control
(Salicylic acid) 0 - - 7.75

As can be seen in Table 6, it can be seen that the area of the Clear Zone is increased in a concentration-dependent manner in both the samples of Preparation Examples and Examples, so that there is antimicrobial activity against Propionibacterium acnes. The complex extracts of the examples of the present invention exhibited very excellent antibacterial activity compared to the extracts of the preparation examples. Among them, it was confirmed that Example 5 had the most excellent antibacterial activity against Propionibacterium acnes.

Example 6: Preparation of serum containing complex extract

A serum containing the complex extract (Example 5) was prepared according to a conventional method with the composition and contents shown in Table 7 below.

Raw material Content (% by weight) Complex extract (Example 5) 1.0 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Mineral oil 10.0 Sorbitan Stearate 0.5 Glycerin monostearate 1.0 Stearic acid 1.5 Glyceryl Stearate/Pig-400 Stearate 1.0 Propylene glycol 3.0 Carboxypolymer 0.1 Triethanolamine 0.1 Phenoxyethanol Appropriate amount Purified water To 100

Example 7: Preparation of Cream Containing Complex Extract

A cream containing the complex extract (Example 5) was prepared according to a conventional method with the composition and contents shown in Table 8 below.

Raw material Content (% by weight) Complex extract (Example 5) 1.0 Shea Butter 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Mineral oil 10.0 Dimethicone 3.0 Sorbitan Stearate 0.5 Glycerin monostearate 1.0 Cetearyl alcohol 2.0 Glyceryl Stearate/Pig-400 Stearate 1.0 Propylene glycol 3.0 Carboxypolymer 0.25 Triethanolamine 0.25 Phenoxyethanol Appropriate amount Purified water To 100

Comparative Example 1: Preparation of Cream Containing General Dogwood Extract

A cream containing the extract (Preparation Example 1) was prepared according to a conventional method with the composition and contents shown in Table 9 below.

Raw material Content (% by weight) Extract (Preparation Example 1) 1.0 Shea Butter 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Mineral oil 10.0 Dimethicone 3.0 Sorbitan Stearate 0.5 Glycerin monostearate 1.0 Cetearyl alcohol 2.0 Glyceryl Stearate/Pig-400 Stearate 1.0 Propylene glycol 3.0 Carboxypolymer 0.25 Triethanolamine 0.25 Phenoxyethanol Appropriate amount Purified water To 100

Test Example 6: Confirmation of skin irritation relief effect

After inducing skin irritation through the tape stripping method on the inner part of the lower arm for 10 men and women in their 20s to 30s who do not have skin allergy symptoms, bitter mugwort, panworm, sedum, seaweed according to the present invention and The degree to which the irritation caused by applying the cosmetic composition containing the dogtail extract and the cosmetic composition not contained was evaluated relative to each other. First, the two inner parts of the lower arm were taped 30 times with regular scotch tape to induce skin erythema. After inducing erythema, a cosmetic composition containing the complex extract according to the present invention (Example 7), a cosmetic composition containing the extract of Preparation Example 1 (Comparative Example 1), and a cosmetic composition containing no extract (Control Example), respectively Applied. The degree of skin irritation was evaluated 3 hours after application.

<Evaluation of skin irritation degree (erythema)>

0 (no reaction) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear border but weak erythema) 4 (clear erythema) 5 (severe erythema)

division Skin irritation Left Ooh Example 7 0 0 Comparative Example 1 2 3 Contrast 3 2

As can be seen in Table 10, the cosmetic composition containing the complex extract of bitter wormwood, beom fan, sedum, seaweed odor and dogtail as an active ingredient of Examples of the present invention irritates skin compared to Comparative Examples and Comparative Example 1 that do not contain the complex extract. The relaxation effect was excellent.

Test Example 7: Atopic symptom improvement test in human body

In order to confirm the effect of improving the atopic symptoms by the bitter mugwort, beetroot, sedum, seaweed and dog stingray complex extracts of the examples of the present invention, the cream of Example 7 was applied to the panels to evaluate the degree of improvement of atopic dermatitis.

Thirty panelists who thought they had symptoms of atopic dermatitis with similar symptoms were selected and divided into 10 people. At a temperature of 24 to 26° C. and a humidity of 60%, saline and the creams of Comparative Examples 1 and 7 were applied to the face twice a day for 6 weeks. After the test was over, subjects were asked to fill out a questionnaire to conduct a subjective evaluation of efficacy.

At the time of the questionnaire, symptoms of atopic dermatitis such as "pruritus", "dryness", "keratin development", "dandruff", "erythema", "swelling", "skin cracks", "sickness and eczema", and "Lichenflower" "After evaluating the degree of improvement based on symptoms such as "back," the average was averaged to evaluate the atopic symptom improvement effect for each individual. The results are shown in Table 11.

Number of respondents (person) Markedly improved Improving No change worse Saline solution 0 One 8 One Comparative Example 1 0 3 7 0 Example 7 3 4 3 0

From Table 11, it was confirmed that the cosmetic composition containing the present invention bitter mugwort, beom fan, sedum sprout, seaweed odor and dog stilt complex extract was excellent in improving the symptoms of atopic dermatitis.

Claims (7)

Bitter mugwort prepared by extracting with at least one solvent selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol, and then hydrolyzing with β-glucosidase, Bitter mugwort, sedum sedum, sedum sedum, seaweed odor, and sedum snail extracts are effective in a mixture of 1~2: 1~2: 1~2: 1~2: 1~2 in weight ratios Cosmetic composition containing as an ingredient. delete delete The cosmetic composition of claim 1, wherein the composite extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition of claim 1, wherein the cosmetic composition is for relieving skin irritation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving acne. The cosmetic composition of claim 1, wherein the cosmetic composition is for improving atopic skin.