KR20020095616A - Anti-irritant cosmetic composition containing medical plant extracts - Google Patents

Abstract

PURPOSE: A hypoallergenic cosmetics composition containing a medical plant extract is provided to minimize any harmful effects and poisonous components against human body by using natural medical plant materials capable of preventing an inflammation. CONSTITUTION: The hypoallergenic cosmetics composition consists of 0.001 to 10 weight percent of at least one kind of medical plant extract or mixture thereof selected from a group consisting of corydalis turtschaninovii besser, pycnostelma paniculata schumann, cocculus trilobus, menispermum dahuricum, sinomenium acutum rehder et wilson, melilotus officinalis desr., melilotus suvcolens ledebour, aralia continentalis kitagawa, aralia elata seemann, monotropa unilora L, scopolia parvilora nakai, galium verum L., patrinia scabiosaefolia link, valeriana fauriei briquet, and stachys baicalensis fisch..ex bench, on the basis of 100 weight percent of the composition.

Description

Hypoallergenic cosmetic composition containing herbal extracts {ANTI-IRRITANT COSMETIC COMPOSITION CONTAINING MEDICAL PLANT EXTRACTS}

The present invention relates to a hypoallergenic cosmetic composition, and more particularly to a cosmetic composition, including one or two or more natural extracts of natural herbal materials to prevent skin itching and inflammation.

The surface of the human skin is covered with a stratum corneum, which is naturally mixed with sebum secreted from sebaceous glands and water from sweat glands, so that it remains smooth, shiny and moist. When the water content of the stratum corneum is about 20%, the skin becomes the most ideal and beautiful state.When the water content of the stratum corneum falls below 10%, the keratin is released from the skin and foreign matters are easily penetrated, so that various inflammations and allergies are made. Is induced. In particular, as age increases, metabolism of the skin is not smooth, which causes various inflammations and allergies.

Everyone has a lot of time and money to make beautiful and elastic skin, and make plastic surgery. However, the easiest skin maintenance method is to use a skin composition such as cosmetics. However, at present, more than 5,500 chemical substances such as fragrances, preservatives, and bases are used in the composition for skin, which may cause various side effects by each component (Maibach, HI, Contact Dermatitis, 6; 369-404, 1980). . In particular, as the demand for functional compositions has increased in recent years, various skin cosmetics having various functions have been developed even though there are some side effects on the skin.

Histamine, on the other hand, is the most well-known substance that mediates itching and pain. To date, it has been found that there is no specific receptor for detecting itching, and it is thought to be due to unspecified autonomic nerve endings present in epidermal cells or dermo-epidermal junctions, such as local pain. Itching is transmitted through the slow flow of uncidified nociceptor 'C' fibroblasts through the dorsal horn of the spinal cord, such as pain, and is a harmful receptor that delivers pain and a harmful receptor that delivers itching. The same is not known yet.

Once itching and pain appear to be transmitted along the same neuron. In addition, itching in inflammatory skin diseases is known to play a role in enhancing histamine activity, although histamine protease, interleukin-2, substrate P, and opioid peptides do not act as mediators by themselves (Br J). Haematol 1996 Cancer Biother 1995).

These are substances that mediate itching and pain, mainly from mast cells, and by the cross-linking of FCεRI receptors with high affinity. Histamine may also be released from skin mast cells by inflammatory mediators such as substrate P, stem cell factor (c-kit ligand), vasoactive intestinal peptide and the like apart from FCεRI receptor.

On the other hand, materials used to alleviate irritation and inflammation to date are nonsteroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, and indomethacin. Prednisolone, dexamethasone, etc. are the steroidal systems, but most of them have problems in terms of safety on skin and stability when formulating skin compositions (Kim Chang-jong, Pathology, Hallym) Corp., pp. 61-69, 1988), is researching and developing substances with less side effects and stimulating effects on the human body (Yakhak Hoeji 41 (5) 565-570, Arch.Pharm Res 21 1998 406-410).

Cocculus trilobus (Thunb.) DC is a vine-growing tree that grows in sunny places such as Hwanghae-do and Gangwon-do in Korea.Triloban, isotrilobine, homotrilobin, trilobamin, monomenariline, di It is known to contain biscock clarine alkaloids such as trolline and cocklovin and quaternary salts such as amino trilobin and quaternary salt groproline (Yao Xue Xue Bao 1991). It has been shown to have anticancer activity (Chem Pharm Bull 1987). In addition, it has been used for the treatment of neuropathy, rheumatoid arthritis, gonorrhea and cystitis by rooting during the treatment of motion.

The bird sand vine (Menispermum dahuricum DC.) Is a vine-growing bush that is also called kaleidoscopic vines. It resides in sunny foothills all over Korea and is known to contain alkaloids, taurisin, and terandrin in its roots and stems (Yakugawa Zasshi 1970). In addition, cynomenin and tauricin are known to be effective for hypertension and anti-inflammatory effects, and are currently being studied for the treatment of rheumatism by extracting and classifying total alkaloids (Acta Med Okayama 1976). .

Oriental medicine (Sinomenium acutum Rehder et Wilson), also called chungdeung or Banggi, lives mainly in the mountainous areas of southern Korea and washes its roots cleanly in autumn. Roots and stems are used and have been found to contain alkaloids such as cynomenin, isosinomenin, dicinomenin, L-synactin, aquatamine, beta sitosterol and stigmasterol (Yakugawa Zasshi 1968. 1967), recently Studies involving tumor necrosis factor and anaphylaxis inhibitors from animal cells have been studied (J Ethnopharmacol 2000).

The medicinal herb (Melilotus officinalis Desr.) Is a biennial herb with yellow flowers at the ends of the branches, which grows in low mountainous areas, and has been used to soften outposts and soften tumors of swells and calluses in motion. Root components include coumarin, coumaric acid, dicoumar, etc., and research on anti-inflammatory and anti-edema using them has been conducted (Arzneimittelforschung 1971). In addition, the efficacy of anticancer drugs has recently been revealed and research for this is being conducted (Clin Ter 1999).

Melilotus suvcolens Ledebour is a short biennial grass that grows around the country and is used in the fall. It is known to contain dicoumar, coumaric acid, melanocyte glycosides, saponins, trypsin, beta saponins and trypsin derivatives (Adv Exp Med Biol 1996). Dicoumarin is used to increase blood clotting and peripheral blood circulation (Med Monatsschr 1971) and to increase blood count in leukopenia. In addition, the healing effect of the skin edema inhibition test in rats has been studied (Nippon Ya. Kurigaku Zasshi 1983). In agreement, it may be used in the treatment of eye diseases and hypertension, and may be used as a substitute for Dansam root.

Aralia elata Seemann, also called elm tree, is a 3-4 m high leafy shrub with thorns and produces black round fruit in July-August. It is also divided into a round leaf arbor (var. Rotundata) and a leaf (var. Canescens) without spiny hairs. In the root bark, saponin glycosides, aratoside-E, H, I, J, and K-taralin (Chem Pharm Bull 1995) and many others have been identified (Chem Pharm Bull 1996). In general, alkaloids enhance the redox process of tissues and the secretory function of glucocorticoids of adrenal glands, and increase the resistance of organisms and the phagocytosis of white blood cells. In addition, it is widely known to be effective in treating periodontal tissue inflammation and to increase blood sugar. Recently, olenoic acid and oligoglucoids have been used to measure plasma glucose levels (Chem Pharm Bull 1996). In motion therapy, soreness has been used to treat inflammation, and also for fever and menstrual irregularities.

Solnamul (Galium verum L.) is a perennial plant that blooms yellow flowers in summer and grows wild in Korea. Alkaloids such as fulllavonoids, coumarins, and quinones are known in the roots of the outpost (C R Acad Sci Hebd Seances Acad Sci D 1972, Farm Zh 1976).

In the private sector, root decoctions have been used to treat convergence, antipyretic fever, and inflammation, as well as to treat burns, swelling of legs and cuts.

Matari (Patrinia scabiosaefolia Link) is called soybean because it smells of soy in dry roots. It is a perennial grass that inhabits all over the country and lives on dry dry land. Outpost is used for medicinal purposes, and there are ursolic acid, olenoic acid, and tryptophenoids (J Nat Prod 1997, Chem Pharm Bull 1993), and some of its clinical applications, especially for rooting action from roots, are known (J Tradit Chin Med 1986).

Valerian fauriei Briquet, also known as seungchocho, drunk, is a summery, rooted with perennial grasses that inhabit a few wetlands. The root contains 1.5-8% of essential oils, and it is known that the difference in content varies depending on where it grows. It is also known to contain alkaloid hitanine, valerianin and iridio glycosides (Phytochemistry 1992). Studies using these ingredients are being actively conducted (Chem Pharm Bull 1995 Tokyo). Root stems in the treatment of synergism weaken the central nervous system, reflexes and weaken smooth muscle spasms, dermatitis treatment, jingyeong, sedation is known to have.

Seokchangpo (Stachys baicalensis Fisch.ex Benth) is native to central and southern Korea, and lives mainly in waterside. It is known that all outposts are effective in synergistic treatment, but among them, rhizome is used for sedation and sedation, and is used for treating inflammation and gastritis. There are 0.5-0.9% essential oils in the rhizome, 63-81% of which is known to be beta-asaron (Yakugaku Zasshi 1970). In addition, these essential oils are known to have antifungal activity (Zhongguo Yao Li Xue Bao 1983), and recently, antibacterial activity (Arch Pharm 1998).

Corydalis turtschaninovii Besser (C. remota Fisch.) Is also called pale yellow, early bloom, or differently depending on the shape of the leaf. It is a short perennial herb and inhabits wet places around the country. It is used as a medicinal herb when it blooms and leaves in spring. Histamine free inhibition tests involving alkaloid dihydro cordalin, diglausin and hydrocortisin in the roots have been conducted and are known to have anti-inflammatory activity (Bio Pharm Bull 1994). In addition, it has recently been found to be effective in treating allergens 1-4 (Bio Pharm Bull 1995. Bio Pharm Bull 1997). Pain in the treatment of motion can also be used to treat, calm, calm and nerve.

Aralia continentalis Kitagawa, also called Medurd, is a perennial herb that produces black berries in autumn. Roots have been used in the treatment of copper, and it has been used for a long time to suppress inflammation and palliative action by ingesting its outposts in the private sector. It has been used for febrile diseases, rheumatism and neuropathy. Known ingredients include beta sitosterol glycosides and stearic acid (Zhonggue Zhon Yao Za Zhi 1989), and studies on inflammation-inhibiting activity have been studied in Korea (Planta Med 1995).

There are no known efficacy studies on Pycnostelma paniculata (Bunge) Schumann, Monotropauniflora L, Scopolia parviflora Nakai, but they contain alkaloids such as pheonol and coumar. A number of studies are known (Redox Rep 2000, Fitoterapia 2000, Microbiology 1998, Eur J Pharmacol 1992, J Immunoassay 1992, etc.). These herbs are also known to be used for the treatment of myrrh and inflammation in the treatment of sinus.

An object of the present invention, by overcoming the problems of irritation relief and inflammation relief substances currently used in cosmetics, by using a natural herbal material that is excellent in safety and can prevent skin itching and inflammation, toxicity and side effects on the human body This very little hypoallergenic cosmetic composition is obtained.

In order to achieve the above object, according to the present invention, Hyun-ho-Rye, Sanhaebak, Dang vine, Bird sand vine, Oriental medicine, Medicinal herb, Tumbler, Pole elm, Arsenal, Stinkweed, Poison root, Sprout, Provided is a hypoallergenic cosmetic composition comprising 0.001 to 10% by weight of one or more medicinal herb extracts selected from the group consisting of matari, basket sprouts, and spearmint, based on the total weight of the composition.

The present invention is described in more detail as follows.

The present inventors conducted a lot of tests on a number of natural medicinal plants in order to find a substance with less side effects to the human body and has a stimulating effect. As a result, Hyeonho-ri, Sanhaebak, Dangsu vine, Bird sand vine, Oriental medicine, Yakdong dongsaeng, Sakura, Pole root, Elm tree, Sageweed, Poison root grass, Sprouts, Matari, Basket greens, Sukchangpo This very excellent thing was found out and this invention was completed.

The natural herbal containing composition is specifically,

a) Group consisting of Corydalis, Sanhaebak, Dandelion vines, Bird sand vines, Oriental medicine, Yakdong dongsae, Yeonsi dongs, Pond, Arbor, Stinkweed, Poison root grass, Sprouts, Matari, Cannabis and Sukchangpo 1 medicinal herbs selected from or mixed with two or more herbs to dry until no change in weight of the plant and grinding using a 5-40 mesh filter network,

b) extracting the ground material with a solvent,

c) filtering the extraction stock solution,

d) concentrating the filtered extraction stock solution,

e) freeze drying the concentrated extract stock to dry powder it;

f) formulating the dry powder.

Extracted solvent of the powdered material is purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane One or two or more solvents selected from the group consisting of are used.

As a natural material with stimulating effects such as Hyunho Haeng, Sanhaebak, Dadang vine, Bird sand vine, Oriental medicine, Yakdong dongsa, electric cultivation, P. elm, elm, sage grass, poison roots, solmul, matari, Basket sprouts, extracts of Seokchangpo may be used alone or in combination of two or more. In addition, when two or more are used in combination, more excellent effects can be obtained.

Such herbal extracts can be added to hypoallergenic cosmetics such as flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, pack and the like, and the amount is preferably 0.001 to 10 wt% based on the total weight of the composition. If it exceeds 10% by weight, the composition is poor in the preparation of the composition, and if it is less than 0.001% by weight, its effect is insufficient.

The following examples are provided to illustrate the present invention, but the present invention is not limited thereto.

(Example)

Example 1

100 g of Corydalis was washed, dried and crushed with a 5-40 mesh filter net. 500 ml of purified water was added to this natural herbal raw material, and aged at room temperature for 8 weeks to extract the active ingredient. After 8 weeks, the mixture was filtered through a 300 mesh sieve and concentrated by shaking heating at 100 to 110 ° C. for 5 hours. After cooling, the mixture was subjected to high frequency treatment for 24 hours, filtered at room temperature with 0.45 μm sterile filter, and the filtrate was pre-cooled to about −23.3 ° C. and freeze-dried by Freeze Dry Co Model 2000 to obtain a powder.

Example 2

A freeze-dried powder was obtained in the same manner as in Example 1 except for using acid seaweed as a natural herbal raw material.

Example 3

A freeze-dried powder was obtained in the same manner as in Example 1 except that Dagvine was used as a natural herbal raw material.

Example 4

A freeze-dried powder was obtained in the same manner as in Example 1, except that new sand vines were used as natural herbal raw materials.

Example 5

A freeze-dried powder was obtained in the same manner as in Example 1 except that herbal medicine was used as a natural herbal ingredient.

Example 6

A freeze-dried powder was obtained in the same manner as in Example 1 except that the medicinal herb was used as a natural herbal ingredient.

Example 7

A freeze-dried powder was obtained in the same manner as in Example 1 except for using an electrophoresis as a natural herbal raw material.

Example 8

A freeze-dried powder was obtained in the same manner as in Example 1 except for using chopped mulberry as a natural herbal raw material.

Example 9

A freeze-dried powder was obtained in the same manner as in Example 1 except for using arbor as a natural herb raw material.

Example 10

A freeze-dried powder was obtained in the same manner as in Example 1, except that Saccharus was used as a natural herbal raw material.

Example 11

A freeze-dried powder was obtained in the same manner as in Example 1, except that poison root grass was used as a natural herbal ingredient.

Example 12

A freeze-dried powder was obtained in the same manner as in Example 1 except that sol-namul was used as a natural herbal raw material.

Example 13

A freeze-dried powder was obtained in the same manner as in Example 1 except that matari was used as a natural herbal raw material.

Example 14

A freeze-dried powder was obtained in the same manner as in Example 1 except that basket herbs were used as natural herbal raw materials.

Example 15

A freeze-dried powder was obtained in the same manner as in Example 1, except that spearmint was used as a natural herbal raw material.

Example 16

A freeze-dried powder was obtained in the same manner as in Example 1, except that 33.3 g of amorrhoids, 33.3 g of mulberry, and 33.4 g of kaleweed were used as natural herbal ingredients.

Example 17

A freeze-dried powder was obtained in the same manner as in Example 1, except that 33.3 g of acid seaweed, 33.3 g of Oriental Herbs, and 33.4 g of Sol-namul were used as natural herbal ingredients.

Example 18

A freeze-dried powder was obtained in the same manner as in Example 1, except that 50.0 g of dried vines and 50.0 g of basket herbs were used as natural herbal raw materials.

Example 19

A freeze-dried powder was obtained in the same manner as in Example 1, except that 50.0 g of new sand vines and 50.0 g of spearmint were used as natural herbal raw materials.

Example 20

A freeze-dried powder was obtained in the same manner as in Example 1, except that 50.0 g of medicinal herbs and 50.0 g of arbor were used as natural herbal ingredients.

Example 21

A freeze-dried powder was obtained in the same manner as in Example 1, except that 33.3 g of poison root grass, 33.3 g of matari and 33.4 g of electric loin were used as natural herbal ingredients.

Examples 22-42

Lyophilized powders were obtained in the same manner as in Examples 1 to 22, except that ethanol (70%) was used as the extraction solvent.

Preparation Examples 1 to 21

Each of the purified water extract lyophilized powders obtained in Examples 1 to 21 was contained in an amount of 1.0% by weight based on the total weight of the composition, thereby preparing a cosmetic composition having the composition shown in Table 1 below.

Preparation Example 22-42

Each of the purified water extract lyophilized powders obtained in Examples 1 to 21 was contained in an amount of 0.1% by weight based on the total weight of the composition, thereby preparing a cosmetic composition having the composition shown in Table 1 below.

Preparation Example 43-63

Each of the purified water extract lyophilized powders obtained in Examples 1 to 21 was contained in an amount of 0.01% by weight based on the total weight of the composition, thereby preparing a cosmetic composition having the composition shown in Table 1 below.

Preparation Example 64 ~ 84

The ethanol 70% extract lyophilized powders obtained in Examples 22 to 42 were each contained 1.0 wt% based on the total weight of the composition to prepare a cosmetic composition having the composition shown in Table 1 below.

Preparation Example 85 ~ 106

0.1% by weight of the ethanol 70% extract lyophilized powder obtained in Examples 22 to 42 were respectively contained based on the total weight of the composition to prepare a cosmetic composition having the composition shown in Table 1 below.

Preparation Example 107-127

The ethanol 70% extract lyophilized powders obtained in Examples 22 to 42 were each contained 0.01% by weight based on the total weight of the composition to prepare a cosmetic composition having the composition shown in Table 1 below.

Comparative Production Example 1

To prepare a cream composition of the composition shown in Table 1 does not contain a natural product extract.

Preparation Example 128

Flexible Cosmetics

1.0 wt% of the purified water extract lyophilized powder obtained in Example 1 was contained, thereby preparing a cosmetic composition having the composition shown in Table 2 below.

Preparation 129

pack

1.0 wt% of the purified water extract freeze-dried powder obtained in Example 1 was contained to prepare a cosmetic composition having the composition shown in Table 3 below.

Test Example 1

Histamine anti-inhibitory test

The histamine anti-inhibitory test was conducted using the cosmetic compositions of Preparation Examples 1 to 127 containing natural herbal extracts and the cosmetic compositions of Comparative Example 1 containing no herbal extracts. Using Comparative Example 1, which did not add the extract as a control, the experimental animals were divided into groups.

As the experimental animals, rat sprague-dawley (male, 10 weeks old) 300-350 (g / rat), which is mainly used as a biomedical research model including toxicology and pharmacology, was purchased and used by Samtaco Bio Korea. Compound 48/80 (sigma C2313) was used as a histamine (Sigma) free inducer.

First, the back of the rat was carefully epilated with a hair remover, and the first 4 histamine injection sites were selected. 0.5 g of the extract-containing cosmetic composition of each group in two positions at four positions, and the other two positions were applied as a control. After 1 hour, the second application was carried out at the same position as the first, and after 1 hour, 0.05 ml of 0.5% histamine was intradermally injected with the base material. After intradermal injection, 1.5% Evans Blue (sigma E2129) was injected intravenously (1.5 ml) to confirm blue infection. After 45 minutes, the rat was cervical distal bone and the skin was carefully dissected. The long and short diameters of the blue spots were measured, respectively, and the area was determined.

Using the obtained blue salt area, the histamine anti-inhibition rate was calculated according to the following formula. The results are shown in Tables 4a to 4f below.

Test Example 2

Egg urinary membrane test

Villi ureter analysis is a test using lower vertebrates, which is not an exact in vivo test, but is widely used as an alternative to conventional animal testing (Council Directive 93/35 / EEC, amending for the 6th time Directive 76 /). 68 / EEC). A typical test method is the method using the chorioallantoic membrane (CAM) of eggs, which is widely used for evaluation of skin irritation and eye irritation (Gettings S.D. et al, 1994: Gilleron L. et al, 1996).

The fertilized egg (White leghorn) was incubated in a fertilized egg incubator (KE-500, EUN JO. Co.ltd), which has an automatic transfer function at 2 hours intervals and maintains 37.5 ± 1 ℃, 40-60% humidity. On the 10th day, unfertilized eggs or undeveloped eggs were screened using a gum, and only those with good development status were selected and used. On day 14, the egg shell membrane was removed and CAM exposed, and then 300 mg of test substance was applied. After application of the test substance, the parameters of bleeding, dissolution, and coagulation reaction time were measured in seconds, and the final evaluation value was obtained by adding time-dependent scores (Bagley et al , 1988; Luepke 1985; Luepke & Kemper, 1986). ). Anionic surfactant (Henkel KGaA D-40191) was used as a control material and the observation time was within 300 seconds in total. Six eggs per each material were used as one group, and the result value (Q) was calculated as the ratio of SCOREsp-test / SCORE _STD -test. Application method was applied to the end point method (End point method) in accordance with the general cosmetic composition.

The results are shown in Tables 5a to 5f below.

<Score judgment>

Less than 6 = very hypoallergenic

6≤Q≤12 = hypoallergenic

12 <Q <16 = stimulus

16 or more = severe stimulation

Test Example 3

Human closed patch test

The human patch test is widely used as a method to search for primary irritants (Matsumura et al , 1995), but dermatologically, it refers to a diagnostic patch test for the diagnosis of allergic contact dermatitis. do.

In the back of 30 healthy adults male and female, 40 μl was added using a kit (Finn chamber), which was fixed to the skin with a scanpore tape. After 24 hours it was removed from the skin and after 4 hours the results were determined. The degree of erythema and edema are represented by numbers in Tables 6a to 6d. The criteria for acceptance were in accordance with the criteria of the International Contact Dermatitis Research Group (ICDRG) (Wooding et al , 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al , 1993).

<Decision Criteria>

0 = no redness

1 = erythema

2 = redness and papules

3 = redness, papules and vesicles

4 = severe edema and vesicles

Stimulus (%) = (responsiveness of positive response / response of self population) × 100

As described above, according to the present invention, it is possible to obtain a hypoallergenic cosmetic composition containing natural herbal extracts useful for the prevention and irritation of the itch due to less side effects such as skin rash and skin allergy.

Claims (1)

Choose from a group consisting of corydalis, wild sea cucumber, dang vine, bird sand vine, oriental medicine, medicinal herb, cultivated wormwood, wormwood, arbor, persimmon grass, poison root grass, solmul, matari, basket herb and quarry A hypoallergenic cosmetic composition comprising 0.001 to 10% by weight of one herbal extract or two or more mixed herbal extracts based on the total weight of the composition.