KR101936150B1 - Composition comprising natural plant extracts for acne treatment - Google Patents

Abstract

The present invention relates to a composition for acne treatment and, more specifically, relates to a composition for acne treatment of a pimple, comprising a Centella asiatica extract, a Portulaca oleracea extract, and a Prunus jamasakura bark extract. According to the present invention, the composition is derived from a natural plant to be safe, has no side effects, and can be helpfully used as a new composition for acne treatment having high treatment activity.

Description

[0001] The present invention relates to a composition for treating acne skin diseases,

The present invention relates to a composition for treating acne, and more particularly, to a composition for treating skin diseases caused by acne including a screening oil extract, a mash extract, and an extract.

Acne is an inflammatory skin disease that causes hairy pores (open and closed papules), pimples, deep boils (cysts or nodules) that clog the face, neck, chest, back, and shoulder. Acne usually occurs in the early teenage years, but symptoms can be severe around the age of 20, and can occur in adults in their 30s and 40s. Generally, even without treatment, acne usually disappears in a few years, but if left untreated, permanent scarring can be left. Acne is not a life-threatening disease, but it puts a psychological burden on the patient, and serious acne can leave a serious, permanent scar, which can be a cosmetic problem. Therefore, it is important to have an interest in skin care and treatment of acne in normal times.

Although the exact cause of these acne is still unclear, several causes are more complex than one. Acne is activated by male hormones in both women and men. Increased male hormones during puberty cause the sebaceous glands of the skin to grow larger (skin oil glands). The sebaceous glands are present in many areas of the face, back, and chest where acne is common. The sebaceous glands are connected to a tube containing hair called a hair follicle, which produces an oil substance called sebum. The sebum is released to the skin surface through the opening of the hair follicle. The sebaceous follicles stimulate the inner wall of the hair follicle, causing the inner wall cells to fall out more quickly, and the missing cells are clogged to form holes in the hair follicles, forming the basic lesion of acne (comedone, hardened sebaceous glands). Propionibacterium acnes, a germ that resides in the hair follicle, secretes lipolytic enzymes. This enzyme breaks down triglycerides in sebum to form free fatty acids, which then stimulate the hair follicles again to break down the hair follicles. Finally, sebaceous germs and dislodged cells are released into the skin, resulting in erythema, edema, pus, or pimples. Immunological responses to P. acnes contribute to the inflammatory response of acne. It is well known that acne is a family history. However, its exact genetic pattern is still uncertain.

Propionibacterium acnes is a bacterial bacterium that grows and feeds on sebum, causing it to become a major source of trouble. It is normally present in normal skin and does not cause any problems. However, when the hair follicles become clogged and sebum accumulates in the hair follicles, Eat quickly and grow up. After eating the fat (triglyceride), the fungus excretes a butter-like substance, fatty acids. Such a chemical change breaks the pH balance of the oil on the surface of the skin, alkalizing the weak acidity of the healthy skin, resulting in deterioration of the skin's own function and a vicious cycle of the skin.

In order to prevent and alleviate the acne induced by Propionibacterium acnes, a medicine mainly used or medicine for eating is used. It is a medicine made by transforming topical antibiotics (clindamycin, erythromycin) and vitamin A, which have a direct bactericidal effect against acne bacteria and prevent the production of free fatty acid. (Tretinoin, adrenalin), a strong antimicrobial agent, and acne ointment (benzoyl peroxide), which has a few anti-inflammatory and cotton-soluble effects, . There are two major types of drugs to eat: antibiotics that act to sterilize acne bacillus and reduce inflammation, and retinoids, which are known as roucutan, which are made by modifying vitamin A. Retinoids are known to regulate sebum secretion by reducing sebaceous secretion. However, this medication is not only used to control sebum, but also to treat abnormal keratinization (abnormal keratinization with incomplete and immature keratinization), inflammation reaction, bacterial growth It helps to block all pathways of acne development.

These oral medications use retinoids, corticosteroids, etc. for the purpose of inhibiting the function of the sebaceous gland and inhibiting the destruction of the follicular wall. However, these preparations have side effects such as skin rash and dry skin, It is necessary to pay attention to its use. Therefore, there are many studies on substances that can show such medical treatment effects at the stage of cosmetics before the treatment of acne within the medicinal range, but there are not many such substances. In particular, cosmetics can be used over a long period of time in comparison with therapeutic drugs, and skin damage to normal skin may be small, so it is necessary to develop a cosmetic raw material that is safe for skin and has an excellent acne improvement effect. Therefore, a variety of natural extracts derived from natural plants have been tried, but the mechanism thereof is unclear and the clinical effect is not widely used because of lack of data and proof. Therefore, there is a need for a new composition that is safe and yet fully characterized for its mechanism and clinical effects.

The present inventors have developed a composition capable of fundamentally treating acne skin diseases due to various reasons, which are safe and derived from natural products, and have sought to elucidate not only the mechanism but also the excellent clinical effect for various patient groups.

Accordingly, the present inventors aim to provide a composition for treating a new acne skin disease containing a natural extract as an active ingredient.

In order to accomplish the above object, the present invention provides a composition for treating acne skin diseases, which comprises a screening oil extract, a mash extract, and an extract.

In addition, the present invention provides a cosmetic composition for acne skin, comprising as an active ingredient, a folded-leaf extract, a mashish extract, and an angelfish extract.

As a result of administration of the composition including the screening composition, screening extract and extract of Aspergillus acnes extract according to the present invention, the production of cytokine which is a causative substance was inhibited and the inhibitory effect and antimicrobial effect of the important enzyme of acne formation were proved. It was confirmed that there was a therapeutic effect. The composition of the present invention is useful as a composition for the treatment of a new skin disease derived from a natural substance, which is safe, has no side effects, and has a high therapeutic activity.

1 shows photographs taken before and after administration of the composition of Production Example 3 to subject 1 ((A): before administration, (B): after administration)
2 shows photographs taken before and after administration of the composition of Production Example 3 to subject 2 ((A): before administration, (B): after administration)
3 shows photographs taken before and after administration of the composition of Production Example 3 to subject 3 ((A): before administration, (B): after administration)

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for the treatment of acne skin diseases comprising a screening oil extract, a mash extract, and an extract. In one embodiment, each of the extracts is prepared so that the weight ratio of 1: 0.2 ~ 5: 0.2 ~ 5: 0.2 ~ 5 when the screen wind extract is 1. In one embodiment, the screen wind extract, mash extract, and anthocyanin extract are mixed at the same weight ratio. In one embodiment, the extract is prepared by adding water having a weight of 1 to 50 times the dry weight of each of the screen, mashii, and rhubarb, storing the extract at room temperature for 1 to 5 days, disintegrating the mixture, filtering the concentrate with the filter paper, 0.6 micro-filter. In one embodiment, the extract is extracted with at least one solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms and mixed solvents thereof. In one embodiment, the extract is extracted with 20 to 70% (v / v) ethanol of an alcohol having 1 to 4 carbon atoms. In one embodiment the composition is inhibition of inflammatory cytokine, 5-alpha-reductase (5-a-reductase) inhibition of the activity of the enzyme and the sludge was repeated tumefaciens arc Ness (propionibacterium acnes ) to treat acne because of one or more of the inhibitory effects.

The present invention also relates to a cosmetic composition for acne skin containing a screening oil extract, a mash extract, and an extract of Aspergillus as an active ingredient. In one embodiment, the extract is contained in the cosmetic composition in an amount of 0.01 to 30% by weight. In one embodiment, the composition is any one selected from the group consisting of formulations of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray and a powder.

Centella asiatica (L.) Urbain, Plantae, Anthophyta, Dicotyledoneae, Umbellales, and Umbelliferae Centella. Take a rooted outpost in summer and autumn, remove the debris and dry in the sun. It contains a variety of a-amyrin-type triterpenoids, including asiaticoside, thankuniside, isothankuniside, madecassoside, brahmoside, brahminoside, brahmic acid and madasiatic acid. It also contains flavonoid glycosides composed of meso-inositol, ecntellose (a kind of small sugars), lead, carotenoids, chlorophyll and kaempferol, quercetin and glucose, and rhamnose.

Angiospermae Dicotyledoneae Centrospermales Portulacaceae Portulaca The outpost of a pair of leggings with a pair of leggings. In China, it is the same as in Korea, and in Japan, it is not a process medicine. Machi is the name given to the fact that the leaf shape resembles that of a horse's tooth, and the smoothness of the medicinal material is similar to that of the horse. It is also called "Seokhyeon", which is a leaf with a big leaf and a leaf with a small leaf. It is also an expression of its appearance. It also has a name of long life because the leaf does not well fade for a long time. Also, it is also called 五行 草, meaning that the leaves are blue, the stem is red, the flower is yellow, the root is white, and the seed is black. It is a name attached by having. It is a soft, unusual odor, mucus-like, flavorless, and sour. It is used for bacterial dysentery, swelling, hemorrhoids, cervical lymphadenitis, eczema, fever, uterine bleeding, urine discomfort and the like because it has fever, detoxification and hemostasis. Antimicrobial activity by pharmacological action, intestinal interactions by intestinal smooth muscle contraction, and diuretic effects were reported. Its appearance is a stalk-shaped stem with a spiral-shaped stem with a sparse leaf and the outer surface is yellowish brown or reddish-brown with vertical wrinkles. The quality is easy to break, and the folded surface is yellowish white or yellowish brown. The leaves on the stem are crushed or broken, dark green or dark brown. There is an oval or conical pouch-shaped fruit at the end of the stem of the stem, and it contains a small seed with grayish-brown shine. Other names are Ma, Hwanggak, Shoji lion grass, Mach Chae, Mach Chung, Sanheung, An Dong-chae, ), Ogangcho (方 方 草), Changmyeongchae (長 明 菜), pig grass, thief grass, horse is also called bean. The root of the pepper is white, but it turns red when rubbed for a while. It is famous for its omega-3 fatty acids, a type of unsaturated fatty acid. Boil a light leaf stem of a pair of carrots and let it spice up to make a fresh vegetarian diet stand out. However, pesticides and herbicides sprayed on fields or roadside contaminated material can not be eaten. This is because the stem and leaf of the carpenter store such residues. The species belonging to Portulacaceae belongs to about 16 genera of 500 species in the world and there is a pale species in our country. Chuseong is also a kind of carp, but it is a kind of gardening that grows for ornamental purposes. The name Portulaca is derived from the Latin word meaning 'the fruit is ripe and the lid is opened'. When the fruit is ripe, the lid of the center part falls off, and the seed which is shiny and brilliantly shines in black comes out. When it falls on a moist soil, it germinates the following spring. Species name oleracea (oleracea) is a Latin word meaning 'savory, eatable vegetables'. In Korea, China, and India, it has been eaten since long ago in Europe, and it still boils stems and leaves before it blooms, and is made into herbs or salads. History has also been used as a medicinal product. Hanja Mahi-hye is a medicinal name, meaning that the leaf shape resembles a horse's tooth and is a grasshopper that eats like a bee. The old relationship between humanity and petticoat is because the habitat is farming land. It is a hemerochoren in the agricultural culture and now lives only in the land where it is cultivated. The leaves and stems of leggings are well-developed on the feet with fleshy texture developed by water storage. I do not like dried habitats, but I like the fields rather than the rice fields. This means that when the place to live becomes dry, it can be overcome. Paddy fields are always too humid to live, and they live well on land that is properly moistened or drained well as a field. If you go to a park where you have been in Korea for about a year, it is because of the fact that a pair of leggings occupy a large area like a main character. Direct sunlight glowing is pulled down to the ground where it floats and it survives even if it is struck. It is due to my resistance to heat, and other weeds die and die, but leggings lower their roots from the stalks touching the ground, and the ends of the stalks rise vigorously toward the sky. It seems to look like a firm will. Purses spread out from the edge of the cultivated field in such a way. Purslane is a broad-spectrum species that spreads throughout the world, along with human cultivation (field) culture. The origin of the pairing is the arid region of northern Africa, where humanity must travel from the east of Africa. In the southern part of the peninsula, human beings lived in the Paleolithic period more than 40,000 years ago, and the era of Bronze Settlement agriculture developed greatly after that. Therefore, it is clear that the pairing had already arrived long before the bronze-settling agriculture, and it is not an academic problem even if it treats it as a native species of the Korean peninsula. Purslane is less frequent in marine bio-climate regions where moisture stress does not occur and is more prevalent in typical continental bio-climate regions like the Korean peninsula. In the Atlantic oceanic biological climate regions, such as the United Kingdom, they are not listed or even distributed in rare species. In Japan, it is classified as prehistoric plant species before the historical period, and it is recognized as a species brought along with bronze agriculture culture. It may be a rational interpretation considering the geographical environmental conditions isolated as an island. Our case is at least prehistoric plant species that are earlier than Japan or endemic species. It is still a very old plant name over 500 years old. Purslane is a compound word of 'iron' and 'bilum'. It is more fishy and tougher than Amaranthus lividus, which is a fishy fish. It may be that the prefix "iron" is attached to the meaning of iron or ox. It is said that it is also a sign in the main building of the Buddha. Purslane is a grass with a color that symbolizes the five lines of Yin and Yang five lines from black, blue, red stem, yellow flower, white roots, to be. Therefore, it is also called "五行 草". Depending on the province, it is also called pig grass, thistle grass, horse mackerel, and also used as pig feed. Chungbuk province is called Chungyang province, the green tea grass roots are changed to red when you rub the 'Rohsin tiles' play (November, 2012, like an oak, explains Chung Young-wan). Though the quality is lower than the saplings, the petroglyphs so deeply came into our life from early on, and it was a resource plant located at the center of the herb culture. In Japan, it is called Inubi (dog), and Purslane is called Suuberiu (滑). Though they are a much better plant as a vegetable than a pair of leggings, they regard the plant as a trivial (dog, dog). It is not a resource plant located in their herb culture. The same goes for leggings. The Japanese name Subury Hugh is a name that he later recognized that it is a herb that can be eaten. Subury Hugh is a slippery slipper. It is a name that can be put on after you boil the pendulum with herb. So it becomes clear that Japanese herb culture is rooted in our spontaneous culture of herbivals, living in continental climate regions. You can observe an interesting phenomenon when you observe a pair of leggings growing in a field during a half day. When the temperature rises enough around 8:00 am and the air gets warmer, the flowers start to bloom. One small flower with a small number starts to wilt once in the afternoon and can not be seen after a day. It is a flowering flower. The living things in the sun live by lifting the ground, only the tip of the stem stands upwards. On top of it is a yellow flower. When it is a little shaded, it stays up from the bottom of the stem, and the leaves, flowers, and fruit trees run less than those that grow in the sun. Purslane means to produce more flowers and fruits in an extremely dry and hot habitat. In the midst of a summer garden, a pair of carpenters were pulled out of the dilapidated farmer's hand and thrown in the fields, where their survival and dispersal strategy spread out. The tongue-and-plexiglass-shaped red stiletto stalk was a useful props for the ghost-playing game, Japanese children say that they have played such a game with mother-of-pearl, but they can not match the plays made with leggings. The outpost (草 草) is a march, and the seed is a marchon. (1) Maehyeonhyeon ① In summer, when 莖葉 (foliage) is thick in autumn, cut off the outposts, remove impurities and mud, heat them slightly in boiling water, and dry them in the sun. ② Composition: The outpost contains a large amount of noradrenaline (2.5 mg per 1 g of fresh outpost) and a large amount of potassium salt. It also contains dopamine, dopa, malic acid, citric acid, glutamine acid, asparaginic acid, alanine, sucrose, glucose and fructose. In the edible part, the amount of protein 2.3 g per 100 g, fat 0.5 g, sugar 3 g, crude fiber (crude fiber) 0.7 g, potassium 85 mg, phosphorus 1.5 mg, carotene 2.23 mg, vitamin B ₁ 0.03 mg, vitamin B ₂ 0.11 mg, , And vitamin C 23mg. The outposts also contain alkaloids, coumarins, flavonoids, ferric glucosides, anthraquinone glycosides.

Prunus sargentiiRehder is a bark of a mountain cherry tree. It is a broad-leaved large-leafed tree with roots and grows up to 20 meters tall. There is a lot of branches in the middle of the stem, and it spreads obliquely to the side, and the upper part becomes a conical shape like a broom turned upside down. Inhabits in forests, valleys, and beaches of highland 1,600m or less in mountains. It can be seen mainly in the 600m high mountain. The skin of the stem has long horizontal bark like stripes. Between April and May, blossom in pink or white, with young leaves in the leaf running spot, or slightly before the leaves. Two or three flower stalks are gathered, and like the umbrella fern, flowers with a diameter of about 2.5 ~ 4㎝ run on the end. There are five petals. Calyx leaf is divided into 5 branches and is reddish brown. There are many surgeries. There is no scent. Leaves 8 ~ 12㎝ long are hanging on branches. It has an oval shape with a sharp tip and a sharp saw blade-like sawtooth on the edge. When it is young, it has reddish and sticky hairs, and its front side becomes dark green gradually. The back side is white. The petiole is reddish, hairless, and has a pair of reddish-blue nymphs on its upper side. It is yellow in autumn, and dyes in red. Round fruit with seeds sealed with a solid nucleus in May to June is yellowish red to blackish brown and shiny. Young trees are dark reddish brown. It is smooth and there are many peel eyes that are long side. As you stay, the color becomes brown or dark gray, with light gray stains and peeled eyes peeled off to the side. When it becomes a dead wood, its skin divides like shallow scales. The edges are bright yellow. Inside there is a wide core of yellowish brown. In the middle, there is a small yellowish brown color. The branches are reddish purple and gradually reddish and light brown. When you stay, it is purplish and dark gray and has peeled eyes. It is thick and it is a little bit hard. It is a conical shape with a narrow bottom and a pointed end. It is reddish or brownish. Medicinal use: bronchitis, cystitis, enteritis, insomnia edible: fruit stem skin, twigs (杏,, 櫻 皮) Take in spring and dry in the windy shade. Bronze, cystitis, intestinal inflammation 5g dried in water to 700 ml, dalyeoseo drink. Fruit (Yaotong, 櫻 草 花) It is collected in spring ~ summer and dries in the shade where wind is well. 5 g of insomnia dried in water 700 ml, then drink dalyeoseo. The raw cherries are eaten as fruits. It is a deciduous broad-leaved tree that grows in the mountains. The stem divides into several branches from a relatively low position and strikes many branches. Leaves are placed alternately and have an egg length of 5-8cm. The base of the leaf is rounded, the tip is pointed, and small sawtooth is evenly arranged on the edge. The young leaves are reddish and have fine hairs on both sides. The flowers bloom with 2-3 leaves with leaf at the end of short branch and have long peduncle with more than 4cm. The color of the flower, about 3.5cm in diameter, is pale pink. After the flowers are bloomed, they are reddish as they grow in round fruit with a diameter of about 6mm and turn black. It is widely distributed throughout the country and lies in the forests of the mountain forests. Anthopia (櫻 皮). It is also called 火 皮. I use the shell of eggplant as medicine. Cherry blossoms, cherry blossoms, cherry blossoms, and some cherry blossoms. Collect during the growing season and dry in the sun. Peel off the rough parts and cut into small pieces. Theylkinine, Coumarin, Phlorrhizin, Amygdalin, Malic acid and the like are contained. Jinhae, has the efficacy of detoxification. To stop coughing, take 4 to 8 grams of dried medicines at a time with 200 cc of water, or take the medicinal product with a powder.

In addition, the composition of the present invention may further include medicinal materials conventionally added in the art for taste, flavor, etc., in addition to the medicaments described above.

In addition, the composition of the present invention may include extracts prepared by extracting a pharmacological agent for use as an internal or external use. In one embodiment, the composition of the present invention may comprise a mixture of the extracts of each of the medicaments, and in other embodiments may comprise an extract obtained from the mixed medicament.

At this time, the extract of the medicinal material can be produced by a conventional extraction method. That is, a method may be used in which an extraction solvent is added to the above-mentioned medicinal material and extraction is carried out at a high temperature under a low pressure or a high pressure. As the extraction solvent, for example, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent obtained by mixing them can be used. There is no particular limitation on the amount of the solvent to be used, but the amount of the solvent may be about 1 to 50 times the dry weight of the drug substance to be extracted, considering the extraction efficiency, the efficacy or economical efficiency of the composition, and the like.

In one embodiment, the composition of the present invention may be prepared by extracting each medicament (wherein each medicament may be ground or chopped with a grinder prior to extraction) using a suitable solvent and mixing them, Can be extracted together, and the extracts can be prepared in a mixed form from the time of extraction.

Next, the extraction mixture is filtered to remove impurities. The filtration method is not particularly limited, and filtration can be performed using a conventional method in the art. Specifically, an extract in which impurities are removed can be obtained by passing through a microfiltration paper.

Needless to say, it is also possible to use the powder after the powder is prepared by an additional process such as vacuum distillation, freeze-drying, or spray drying.

A specific method for producing the composition of the present invention is as follows.

The dried medicinal composition of the screen, the mashinose and the rhubarb is added with water in an amount of 1 to 50 times the total dry weight, followed by hot extraction. The extracting conditions were as follows: water having a weight of 1 to 50 times the dry weight of each of the screen wind, macheyee and rhubarb, was stored at room temperature for 1 to 5 days and then crushed. The filtrate was centrifuged with a filter paper, .

In one embodiment, the extract is in one embodiment extracted with at least one solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms and mixed solvents thereof. The alcohol may be selected from methanol, ethanol, propanol, isopropanol and butanol, preferably 20 to 70% ethanol aqueous solution or ethanol. More preferably 20 to 70% (v / v) ethanol.

The compositions of the present invention inhibit the inflammatory cytokines, 5-alpha-reductase (5-a-reductase) inhibition of the activity of the enzyme and the sludge was repeated tumefaciens arc Ness (propionibacterium acnes ) to treat acne because of one or more of the inhibitory effects.

Cytokines are factors that regulate intercellular interactions related to immune, inflammatory, or hematopoietic responses, including interleukin-1, interleukin-6, interleukin-8, and tumor necrosis factor (TNF-a). The production and secretion of excessive or uncontrolled cytokines disturbs the immunoregulatory function to mediate or worsen various diseases. Examples of such diseases include allergic diseases, inflammatory diseases, autoimmune diseases, and the like.

Accordingly, the composition for inhibiting cytokine production of the present invention may be useful for the improvement of various skin diseases, particularly for the improvement of acne.

In the composition for inhibiting cytokine production according to the present invention, cytokine is preferably selected from the group consisting of interleukin-8 or tumor necrosis factor (TNF- It can be understood as a).

When the composition for inhibiting cytokine production of the present invention is identified as a cosmetic composition or a pharmaceutical composition in a specific aspect thereof, the effective amount, method of administration, and formulation of the composition are effective as described above.

In another aspect, the present invention provides an external-use composition for treating acne, which comprises extracts of Centella asiatica, Corynebacterium cepa, and Rhizobia.

In addition, the composition according to the present invention may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like oral preparation, external preparation, suppository or sterilized injection solution according to a conventional method Can be used. Specifically, in the case of formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant,

Or may be formulated using excipients.

In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, syrups and the like, and various excipients such as wetting agents, sweeteners, fragrances, preservatives, etc. in addition to commonly used diluents such as water and liquid paraffin . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

The composition of the present invention may contain pharmaceutically acceptable carriers in addition to the centipedium extract, mackerel, and squid extract as active ingredients. Such pharmaceutically acceptable carriers are those conventionally used in pharmaceutical preparations, and include lactose, dextrose, But are not limited to, sucrose, sorbitol, mannitol, starch, acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, Hydroxybenzoate, talc, magnesium stearate, mineral oil, and the like. The pharmaceutical composition of the present invention may further comprise, as an additive, a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative and the like.

The carrier may be included in the pharmaceutical composition of the present invention in an amount of about 1% by weight to about 99.99% by weight, preferably about 90% by weight to about 99.99% by weight, based on the total weight of the pharmaceutical composition, To about 20% by weight.

On the other hand, the pharmaceutical composition of the present invention is directly administered to the skin by a local administration method.

The pharmaceutical composition of the present invention can be prepared in unit dose form by formulating it with a pharmaceutically acceptable carrier and / or excipient or can be manufactured by inserting it into a multi-dose container. The formulations may be in the form of a solution, suspension or emulsion, or may contain an agent such as an agent, an excipient, a powder, a granule, a tablet, a warning agent, a lotion,

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is in any aspect limited to the scope of the present invention. Should not be construed as doing.

In another aspect, the composition of the present invention can be used in cosmetic compositions for improving skin diseases. The present invention relates to a cosmetic composition for acne skin containing as an active ingredient a screening oil extract, a marigold extract, and an angelfish extract. In one embodiment, the extract is contained in the cosmetic composition in an amount of 0.01 to 30% by weight. In one embodiment, the composition is any one selected from the group consisting of formulations of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray and a powder.

As used herein, the term " acceptable carrier for cosmetic preparations "refers to compounds or compositions that are already known and used in cosmetic preparations, or which are compounds or compositions to be developed in the future and which do not have toxicity, instability or irritability It says.

The carrier may be included in the cosmetic composition of the present invention in an amount of about 1% by weight to about 99.99% by weight, preferably 0.01 to 30% by weight, based on the total weight of the cosmetic composition. However, since the ratio depends on the formulation described above in which the cosmetic composition of the present invention is prepared and on its specific application site (face, neck, etc.) or its desirable application amount, And should not be construed as limiting the scope of the invention.

The composition of the present invention can be used in cosmetics, nutrition lotion, nutritional essence, massage cream, beauty bath additive, body lotion, body milk, bath oil, baby oil, baby powder, shower gel, shower cream, sunscreen lotion, Skin lotions, skin creams, sunscreen cosmetics, cleansing milks, hair removal products, facial and body lotions, face and body creams, skin whitening creams, hand lotions, hair lotions, cosmetic creams, jasmine oils, And can be manufactured in the form of water, soap, shampoo, hand cleanser, medicinal soap {non-medical use}, cream soap, facial wash, hair rinse, cosmetic soap, tooth whitening gel, tooth paste and the like. To this end, the compositions of the present invention may further comprise suitable carriers, excipients or diluents conventionally used in the preparation of cosmetic compositions.

The carrier, excipient or diluent which may further be added to the cosmetic composition of the present invention include purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, humectant, thickener, antioxidant, Buffers, lower alcohols, and the like, but are not limited thereto. Further, if necessary, it may contain a whitening agent, a moisturizing agent, a vitamin, an ultraviolet screening agent, a perfume, a dye, an antibiotic, an antibacterial agent, and an antifungal agent.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosifying agents, emulsifiers, stabilizers, sunscreens, coloring agents and flavoring agents.

As the oil, hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil and avocado oil may be used. Examples of the wax include wax, wax, carnauba, candelilla, montan, ceresin, liquid paraffin, Can be used.

As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used As fatty acid esters, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used. As the surfactant, a cationic surfactant, an anionic surfactant and a nonionic surfactant known in the art can be used, and a surfactant derived from a natural material is preferably used.

In addition, it may contain a hygroscopic agent, a thickening agent, an antioxidant and the like widely known in the field of cosmetics, and the kind and amount thereof are well known in the art.

The composition of the present invention can be identified as a soap composition in another specific embodiment.

When the composition of the present invention is recognized as a soap composition, the soap composition of the present invention can be prepared by incorporating a centipede extract and a coriander extract into a soap base, and further comprises a skin moisturizer, an emulsifier, a water softener, .

Examples of the soap base material include vegetable oils such as palm oil, palm oil, soybean oil, pharmacopoeial olive oil and palm kernel oil or animal fats such as tallow, lard, sunshine and fish oil. Examples of the skin moisturizers include glycerin, erythritol, polyethylene glycol, Propylene glycol, hexylene glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used. Examples of the emulsifier include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts and the like As the water softening agent, tetrasodium ethylenediate etc. may be used.

The soap composition of the present invention may further include an antibacterial agent, a dispersant, a foam inhibitor, a solvent, an inhibitor in water, a corrosion inhibitor, a perfume, a dye, a sequestering agent, an antioxidant, an antiseptic and the like as an additive.

In the soap composition of the present invention, the soap base and additives may be included in amounts commonly used in the art, and the soap base generally comprises 99.999% by weight to 50% by weight based on the total weight of the soap composition And the additive may be added in an amount of 1 wt% to 20 wt%.

Best Mode for Carrying Out the Invention Hereinafter, the functions and effects of the present invention will be described in more detail through specific embodiments of the present invention. It is to be understood, however, that these embodiments are merely illustrative of the invention and are not intended to limit the scope of the invention.

Preparation of water extraction composition

100g of each well-dried red clover, mashishi and ansaki were cut to 2-3cm each, and 1kg of water was added to each, and then the mixture was stored at room temperature for 3 days. Thereafter, the mixture was uniformly crushed using a mixer, then filtered through a filter paper and concentrated. The crushed concentrate was centrifuged again at 3000 rpm, and the precipitated solid was discarded, only the confluent solution was collected, and then filtered with a 0.45 micrometer filter to prepare a final extract. The extracted extracts were then mixed.

Preparation of Alcohol Extraction Composition

100 g of each well-dried housepot, Mashishi, and anpani were pulverized, and then the mixture was placed in 1 L of 30% ethanol and allowed to settle at room temperature for 5 days. The mixture was filtered at 300 mesh and stored at room temperature for 5 days. Lt; / RTI > Each of the filtrates was concentrated using a vacuum concentrator. This concentrate was dissolved again in a 30% solution of 1,3-butylene glycol to give a concentration of 2%, and then mixed.

Preparation of Alcohol Extraction Composition

100 g of each well-dried bedspread, michi-hyun, and mouthpieces were pulverized and then put in 1 L of 60% ethanol for 5 days at room temperature. The mixture was filtered at 300 mesh and stored at room temperature for 5 days. Lt; / RTI > Each of the filtrates was concentrated using a vacuum concentrator. This concentrate was dissolved again in a 30% solution of 1,3-butylene glycol to give a concentration of 2%, and then mixed.

[Manufacturing Example]

Cosmetic products were prepared from the extract mixture prepared in the above Example in the following composition (% by weight). Ingredients 1 to 9 in the following Table 1 were mixed and dissolved at 70 占 폚 to form a water part, and Ingredients 10 to 16 were dissolved at 70 占 폚 to form an oil part. The oil part was added to the water part and agitated with a homomixer (Tokushu Kika, Japan) for primary emulsification, and component 17 was gradually added. After the air bubbles were removed, the mixture was cooled to room temperature to prepare a cosmetic composition of a nutritional lotion formulation.

ingredient Production Example 1 Production Example 2 Production Example 3 Comparative Example 1 One Purified water (Until it reaches 100) (Until it reaches 100) (Until it reaches 100) (Until it reaches 100) 2 glycerin 8.0 8.0 8.0 8.0 3 Butylene glycol 4.0 4.0 4.0 4.0 4 Hyaluronic acid extract 5.0 5.0 5.0 5.0 5 Beta Glucan 7.0 7.0 7.0 7.0 6 Carbomer 0.1 0.1 0.1 0.1 7 Germination soy extract 3 3 3 - 8 Magnolia bark extract 3 3 3 - 9 The extract of the present invention 3 (Example 1) 3 (Example 2) 3 (Example 3) - 10 Caprylic Capricc
Triglyceride 8.0 8.0 8.0 8.0 11 Squalane 5.0 5.0 5.0 5.0 12 Cetearyl glucoside 1.5 1.5 1.5 1.5 13 Sorbitan stearate 0.4 0.4 0.4 0.4 14 Cetearyl alcohol 1.0 1.0 1.0 1.0 15 antiseptic Suitable amount Suitable amount Suitable amount Suitable amount 16 incense Suitable amount Suitable amount Suitable amount Suitable amount 17 Triethanolamine 0.1 0.1 0.1 0.1

Meanwhile, for use as a cosmetic composition of the comparative example, a cosmetic composition not containing the extract of the present invention was prepared as shown in Table 1 above.

[Formulation Example 1]

Nutritional lotion (milk skin lotion) manufacturing

The nutritional lotion (Milk Skin Lotion) of Table 2 was prepared using the extract composition prepared in the above example.

ingredient Content (% by weight)  Purified water (Until it reaches 100) glycerin 8.0 Butylene glycol 4.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Carbomer 0.1 The extract of Example 1 3 Caprylic capric triglyceride 8.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

[Formulation Example 2]

Manufacture of nutrition cream

The nutritional creams of Table 3 were prepared using the extract compositions prepared in the above Examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 The extract of Example 2 3 Caprylic capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1

[Formulation Example 3]

 Manufacture of massage cream

The massage compositions of Table 4 were prepared using the extract compositions prepared in the above Examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 8.0 Butylene glycol 4.0 Hyaluronic acid extract 45.0 Beta Glucan 7.0 Carbomer 0.1 The extract of Example 1 3 Caprylic capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount paraffin 1.5

[Formulation Example 4]

 Manufacture of packs

The packs of Table 5 were prepared using the extract compositions prepared in the above examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 The extract of Example 2 4 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount ethanol 6.0

[Formulation Example 5]

 Manufacture of ointment

The ointments of Table 6 were prepared using the extract compositions prepared in the above examples.

ingredient Content (% by weight) Purified water (Until it reaches 100) glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 The extract of Example 3 3 Caprylic capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Wax 4.0

 [Formulation Example 6]

Manufacture of oil products

The oil of Table 7 was prepared using the extract composition prepared in the above example.

ingredient Content (% by weight) Squalane 5.0 Macadamia nut oil 5.0 Jojoba oil 1.0 Walden Colostrum 0.5 Wild rose seed extract 20.0 The extract of Example 3 4 Carlyric capric triglyceride 30.0 Liquid paraffin Balance incense Suitable amount Other Oil (Until it reaches 100)

[Experimental Example 1]

Inflammatory Cytokine  Production inhibitory effect

Using the monocytic THP-1 cell lines derived from humans, the extract compositions of the examples were treated with the concentrations of the following Tables 8 and 9 to obtain Propionibacterium (IL-8) and tumor necrosis factor-alpha (TNF-a), which are inflammatory cytokines induced by the acnes (P. acnes ) strain. Human monocytic THP-1 cells were cultured in a medium supplemented with 10% fetal bovine serum at 37 ° C and 5% CO2 in a Roswell Park Memorial Institute 1640 medium (RPMI 1640). After culturing the cells at a concentration of 10 ⁶ cells / well (24-well plate) and confirming cell adhesion, the P. acnes strain was added to the cell culture medium to induce the production of inflammatory cytokines. The test substance was added at the same time and cultured for 2 days. Here, the test substance is an extract composition of the example which is diluted to the test concentration with the cell culture liquid and mixed in the same ratio. Inflammatory cytokines present in the culture medium were measured using an ELISA (Enzyme-Linked Immunosorbent Assay) kit capable of analyzing each cytokine. The inhibition rate of the production of inflammatory cytokines was calculated according to the following equation, and the results are shown in Tables 8 and 9.

Inhibitory rate of inflammatory cytokine production (%) = 100- (amount of production in the presence of each test substance / amount of production of control group of P. acnes × 100)

Test substance (treatment concentration) % Inhibition Extract of Example 1 1% 17.2 Extract of Example 2 1% 15.3 The extract of Example 3 1% 28.9 P. acne (control) 0

Test substance (treatment concentration) % Inhibition Extract of Example 1 1% 16.5 Extract of Example 2 1% 8.2 The extract of Example 3 1% 33.6 P. acne (control) 0

From Table 8 and Table 9, it can be seen that the extract composition of Example shows an effect of inhibiting inflammatory cytokine production.

[Experimental Example 2]

5 a- reductase  Enzyme activity inhibitory effect

5 a-reductase is the most important enzyme in male hormone biosynthesis and is the most important factor for the production of pubertal acne through excessive sebum production. 5 By measuring the inhibitory effect of a test substance on a-reductase, indirect effects on sebum production inhibition and acne suppression can be confirmed. The extract composition of Example was treated with culture medium of HEK293 (Human Embryonic Kidney) cell line which is a human 5 a-reductase-secreting cell, and it was determined whether the secretion of 5 a-reductase was inhibited by the treatment of the extract composition of the Example. 5 a-reductase-secreting HEK293 cell line was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum at 37 ° C and 5% CO2. Cells were cultured in a 24-well plate at a concentration of 106 cells / well. After confirming cell attachment, the test substance (the extract of Example 3) was added to the cell culture solution for 24 hours, Lt; / RTI > The culture broth was recovered and treated with 5 a-androstane-3, 5-arostane-3, and 17-dione 5 a-reductase by the action of the substrate 4-androstene-3,17-dione 5 a-reductase according to the method of Baerbel P. et al. (Mol Cell Biochem 270: 201-208, 17-dione. The inhibitory rate of 5 a-reductase enzyme activity was calculated according to the following formula, and the results are shown in Table 10.

Enzyme activity inhibition rate (%) = 100- (amount produced in the presence of each test substance / substrate change amount 100 x 100 in the control group)

Test substance (treatment concentration) Enzyme activity inhibition rate (%) Extract of Example 1 3% 13.1 The extract of Example 2 3% 10.5 The extract of Example 3 3% 41.0 Control group 0

As can be seen in Table 10, the extract compositions of the Examples inhibit 5 a-reductase activity, an enzyme important for sebum production.

[Experimental Example 3]

acne( Propionibacteriumacnes , ATCC6919 ) On the antimicrobial effect

Measurement of the antibacterial effect on acne ( Propionibacterium acnes , ATCC 6919) was carried out according to the Kirby-Bauer standard method. Add 106 ml of acne cultured under anaerobic conditions in a brain heart infusion broth to a flat plate medium (containing 0.8% phytoagar) and mix with petridish. Then, a filter paper disc having a diameter of 10 millimeters (mm) impregnated with the extract composition of the Example was placed on the surface of the medium and cultured under anaerobic conditions at 37 DEG C for 24 hours. The antimicrobial effect was confirmed by measuring the diameter of the clear zone where the growth of acne bacterium was inhibited, and the results are shown in Table 11.

Substance (treatment concentration) Clear Zone (mm) Extract of Example 1 2% 9.3 The extract of Example 2 2% 13.6 The extract of Example 3 2% 22.5 Control 0

As can be seen from Table 11, it can be confirmed that the extract composition of the examples excelled in antibacterial effect against acne bacteria.

Minimum inhibitory concentration ( MIC , minimal inhibitory concentraion ) Measure

Acne bacteria ( Propionibacterium acnes , ATCC 6919) were cultured in anaerobic conditions for 24 hours at 37 ° C in 10 ml of brain heart infusion broth. The extract composition of Example 1 was added to the culture of the acne bacterium at different concentrations, followed by further culturing for 24 hours. The minimum inhibitory concentration (MIC) was determined as the minimum concentration of the test substance in which the growth of the test organism did not occur. The results are shown in Table 12.

Test bacteria MIC (%) P. acnes 0.0125

[Experimental Example 4]

Safety test for human skin

Prior to testing the cosmetic compositions of Preparation Examples 1, 2 and 3 and the cosmetic compositions of Comparative Example 1 for improving acne, the cumulative skin stimulation test of the human skin was carried out to verify skin safety of the compositions. Twenty healthy adults were evaluated in the upper forearm after 1 hour, 12 hours, and 24 hours. The application method is Finn chamber or a similar type of chamber. After applying 15 조성물 of composition per chamber, apply the patch and measure the degree of reaction on the skin every time. And the results are shown in Table 13 below. At this time, the skin condition change was visually observed, and the reaction was classified and evaluated according to the following skin condition evaluation criteria

<Change of skin condition standard>

0: No change

1: Very little change

2: slightly changed

3: A little bit harder

4: Severely changed

5: Extremely changed

Test substance Subject response Subject
(Sex skin type) Subject 1 (male, female) Subject 2 (female, neutral) Subject 3 (female, dry) Time lapse 1 hours 12 hours 24 hours 1 hours 12 hours 24 hours 1 hours 12 hours 24 hours Production Example 1  0  0  0  0  0  0  0  0  0 Production Example 2  0 0 0  0  0  0  One  0  0 Production Example 3  0  0  0  0  0  0  One  0 0 Comparative Example  0  0  0  0  0  0  0  0  0

From the results shown in Table 13, it was found that most of all the compositions exhibited a specific reaction. In the case of Production Example 3, there was some reaction, but no stimulation over time. Accordingly, it was confirmed that the composition of the present invention is safe and less irritating to the skin.

[Experimental Example 5]

Acne improvement test for human skin

80 applicants with acne symptoms on the face and back were divided into four groups each consisting of 20 persons, and each group was provided with the composition of Preparation Example 1, Preparation Example 2 and Preparation Example 3, and the final group was provided with the composition of Comparative Example 1 It was applied to the acne area twice a day in the morning and in the evening for one week. In order to determine the skin improvement effect, the degree of symptom relief of participants was written in the questionnaire, and the result was calculated according to the following formula and shown in Table 14.

Improvement degree = Improved number of subjects / Total number of subjects × 100

Improvement results for acne skin Test substance Itching improvement Skin redness improvement Improved skin inflammation Production Example 3 90.0 (%) 85.0 (%) 95.0 (%) Production Example 2 30.0 (%) 50.0 (%) 50.0 (%) Production Example 1 40.0 (%) 30.0 (%) 30.0 (%) Comparative Example 20.0 (%) 10.0 (%) 10.0 (%)

Table 14 shows that the cosmetic compositions containing each of the compositions of Examples 1 to 3 have an improving effect on the acne skin. However, in particular, the composition of the extract mixture extracted with 60% alcohol has an improvement effect on the acne skin It shows excellence.

In particular, even when the degree of acne skin improvement of the subject to which the composition of Production Example 3 is administered is compared before and after for each of Subjects 1 (male and female), subject 2 (female, neutral), and subject 3 As shown in Fig. 1, Fig. 2 and Fig. 3, excellent improvement effects were confirmed.

While the embodiments of the present invention have been described in detail, the scope of the present invention is not limited thereto. Particularly, the drawings show representative treatment examples. As a matter of course, It is easy to know that a composition comprising an acaricide and a globe as an essential ingredient in the invention has excellent effects in the improvement and treatment of various skin diseases.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (9)

A method for treating acne skin diseases, which comprises extracting a mixture of a screen, an extract, and an extract, wherein the extract is extracted with 60% ethanol and the extract is mixed with the same weight. delete delete delete delete The composition of claim 1, wherein the composition is effective for inhibiting inflammatory cytokines, inhibiting 5-a-reductase activity, and inhibiting propionibacterium acnes RTI ID = 0.0 &gt; acne. &Lt; / RTI &gt; A cosmetic composition for acne skin, comprising as an active ingredient, an extract mixture comprising a folded leaf extract, a marigold extract, and an extract of Angelica keiskei koidz. The composition according to claim 7, wherein the extract mixture is contained in the cosmetic composition in an amount of 0.01 to 30% by weight. The composition according to claim 7, wherein the composition is selected from the group consisting of formulations of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, / RTI &gt;

Images