KR101443182B1 - Oriental medicine extrct for preventing hair loss and promoting hair growth, manufacturing method thereof and cosmetic composition for growing hair using the same - Google Patents

Abstract

The present invention relates to an oriental medicine complex extract having the effects of preventing hair loss and facilitating hair growth, a method to prepare the same, and a cosmetic composition for hair growth using the same. The oriental medicine complex extract of the present invention is selected from oriental medicines having proved safety and also having the effects of hair loss prevention; and the facilitation of hair growth have been proved by an experiment testing the ability to facilitate differentiation and proliferation in hair papilla cells. By containing a mixed component of Eclipta prostrate, Sesamum indicum L., Morus tiliaefolia Makino, Sophora flavescens. Solander ex Aiton, and Cynanchum wilfordii (Max.) Hemsl, or further containing functional components of dried Zingiber officinale and Panax ginseng in addition to the mixed component, the present invention has excellent effects on hair loss prevention and hair growth promotion. Therefore, a cosmetic composition containing the oriental medicine complex extract of the present invention can be provided as an active ingredient for hair growth, and the cosmetic composition can also be applied to various hair products such as hair tonics, hair shampoos, and hair conditioners.

Description

TECHNICAL FIELD [0001] The present invention relates to a herbal medicine composite extract having an effect of preventing hair loss and promoting hair growth, a process for producing the same, and a cosmetic composition for hair growth using the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0001]

The present invention relates to a herbal composition complex extract having hair loss prevention and hair growth promoting effect, a process for producing the same, and a cosmetic composition for hair growth using the same. More particularly, the present invention relates to a cosmetic composition for hair growth, Or a combination of the above ingredients and a functional ingredient of health and ginseng to activate the proliferation of harmful dermal papilla cells in the scalp to thereby prevent hair loss and promote hair growth, a process for producing the same, and a hair using the same To a cosmetic composition for growth.

Hair loss has been regarded as a problem of the age group after the 50s, but it has recently been reported that the hair loss population is increasing in the young people in their 20s and 30s. According to the related industry, about 6 million to 7 million people suffer from hair loss, The number of female hair loss population is increasing rapidly.

Typical causes of hair loss include stress, excessive use of shampoo or mousse, hair and scalp damage caused by using perms or dyeing and hair dryers, imbalance of dandruff or sebum secretion, serious illness due to high fever, anticancer drugs and various antithyroid drugs, Administration, nutritional imbalance, intake of processed foods, and genetic factors, among which the nutritional deficiency of the scalp is the most common cause.

There is a slight difference in male and female hair loss. In male alopecia, more than 95% is due to the excessive secretion of male hormones based on genetic susceptibility. Specifically, testosterone secreted from the body is converted to a substance called dihydro-testosterone (DHT) by a specific enzyme. Testosterone is contacted with the hair follicle dermal papilla cell It is activated by the action of enzymes and changes to the active male hormone DHT. At this time, DHT has a relatively high concentration in the body, thereby facilitating the reduction of the hair follicles, thereby rapidly progressing hair loss.

On the other hand, DHT is the main cause of female alopecia but fortunately, female hormone is secreted in female, and DHT inhibits the destruction of hair follicles relatively.

Various treatments such as topical application, oral dosage form, health supplement, gene transplantation, hair transplantation and alternative medicine have been performed as methods for treating the hair loss phenomenon. However, there is no treatment method or substance showing satisfactory results yet .

Although hair loss is not a life-threatening disease, the market for hair loss prevention or hair growth has been growing steeply due to recent interest in well-being and interest in appearance.

For example, the prescription drug market for hair loss prevention is estimated at 39 billion won in 2009 and around 500 billion won worldwide. The total market for hair loss prevention is reported to be about 800 billion ~ 1 trillion won, and around 6 trillion cases including the relevant markets around the world are formed.

Most of hair loss prevention and hair growth products are applied by direct application to the scalp or by taking a method to explore the natural ingredients that do not contain chemical ingredients to extract the active ingredients beneficial to scalp health is being developed as a product.

As a result, Korean Patent Laid-Open Publication No. 2012-38585, for example, discloses that natural materials include herbal medicine materials. According to Korean Patent Publication No. 2012-38585, hair loss can be prevented without decreasing the function of hair products, Which is capable of providing antimicrobial and antioxidative effects, and which is capable of providing antimicrobial and antioxidative effects, and which is prepared by mixing and extracting Hwangryun, Hwangbyeong, Golden, Hwangjeong, Hwanggi, Baekji, .

According to Korean Patent Publication No. 2012-276620, hair loss prevention and hair growth including purlins, iris, papaya, hanchikcho, seomyeotei, hwasoo, gyeonghwa, white paper, And a herbal composition for promoting hair loss prevention and hair growth disclosed in Korean Patent Laid-Open Publication No. 2010-6197 discloses a composition for promoting hair loss and promoting hair growth, which comprises an extract of Morus albacci, a licorice extract, a wain extract, a gruy extract, a ginseng extract, ≪ / RTI >

In addition, there have been reported a number of compositions for improving the condition of scalp and hair mixed with oriental herb medicine. However, research on biological mechanism for hair loss has not yet been sufficiently carried out. Many unhealthy hair loss prevention and hair growth products are exposed to consumers.

Therefore, it is required to develop hair growth inhibitors and hair growth agents that have proven effectiveness based on scientific understanding of hair growth and cycle control. The discovery of new hair growth potential materials will enable enormous added value in domestic and overseas markets.

Accordingly, the present inventors have made efforts to solve the problems of the prior art. As a result, the present inventors have found that herbal medicines that have been clinically proven safe for use in humans for thousands of years have been selected from the naturally occurring effective substances, The present invention has been accomplished on the basis of this finding by providing a herbal medicine composite extract which is safe to the skin and has hair loss prevention and hair growth promoting effect by selecting a composition proved to be effective in preventing hair loss and promoting hair growth and a hair-like well composition containing the same as an active ingredient .

It is an object of the present invention to provide a herbal medicine composite extract selected from herbal medicine having hair loss prevention and hair growth promoting effect.

Another object of the present invention is to provide a method for preparing a herbal medicine composite extract which can be optimized for preventing hair loss and promoting hair growth.

Another object of the present invention is to provide a cosmetic composition for hair growth comprising an extract of a herbal medicine complex having an effect of preventing hair loss and promoting hair growth as an active ingredient.

The present invention provides a herbal medicine composite extract having a hair loss prevention effect and a hair growth promoting effect, which contains a mixed ingredient of Hansikucho, Blackjumma, Sanshinja, Gosam, and White Sash.

Preferably, the herbal composition complex extract of the present invention further comprises a functional ingredient of health and ginseng in the mixed ingredient, wherein the herbal medicine complex extract is selected from the group consisting of water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol , Propylene glycol, dichloromethane, and hexane.

In the herbal medicinal compound extract of the present invention, the mixed ingredients including Hanshikcho, black jam, fresh sashimi, ginseng and white sourdough are mixed at the same weight ratio among the ingredients, and the additional functional ingredient is mixed with the ingredients of health and ginseng Are mixed in the same weight ratio, but are preferably contained in a ratio of 0.5 to 1 weight based on the mixed components.

The present invention relates to a method for producing a water-in-oil emulsion, comprising the steps of: 1) mixing the ingredients in the same weight ratio between the ingredients and mixing them with water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane And extracting the crude extract with at least one solvent selected from the group consisting of < RTI ID = 0.0 >

2) The crude extract is filtered and purified by any one method selected from filter cloth, filter paper and vacuum filtration apparatus,

3) A method for producing a herbal medicine composite extract having the effect of preventing hair loss and promoting hair growth, including freeze-drying.

In the production method of the present invention, the mixed components of step 1) are mixed in the same weight ratio among the components, and may further contain health and functional ingredients of ginseng. In this case, functional ingredients The ingredients can be mixed.

In the production method of the present invention, the extraction of step 1) is carried out by shaking at 50 to 120 캜 for 2 to 24 hours, and the lyophilization of step 3) is carried out from -20 캜 to stepwise 30 캜 for 24 to 72 hours Lt; / RTI > to obtain a dry powder.

Further, the present invention provides a composition for hair growth comprising 0.001 to 30% by weight of the herbal composition complex extract having hair loss prevention and hair growth promoting effect, which is useful for hair loss prevention and hair growth promotion.

More preferably, the herbal medicine composite extract is extracted with ethanol at a ratio of 1: 1: 1: 1: 1: 0.5: 0.5 by weight of the mixed components of the herb medicine, black jima, And a composition for hair growth which is useful for preventing hair loss and promoting hair growth.

According to the present invention, it is possible to provide a herbal medicine composite extract which has been proved to be safe and has been proved to be effective in hair loss prevention and hair growth.

Accordingly, the herbal medicine composite extract of the present invention provides a herbal medicine composite extract containing a mixture of hanjiccho, blackjumma, sanguinea, gosam and white sachet, or a mixture of the herbal ingredients with health and ginseng ingredients , Activating hair dermal papilla cells, which play a key role in hair growth, and promoting the proliferation of dermal papilla cell proliferation, thereby confirming hair loss prevention and promoting hair growth, thereby providing a cosmetic composition for hair growth containing the same as an active ingredient.

FIG. 1 is a photograph showing the results of an experiment for promoting mouse hair growth using the herbal medicine composite extract obtained under ethanol produced in Example 2 of the present invention. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a herbal medicine composite extract having a hair loss prevention effect and a hair growth promoting effect, which contains a mixed ingredient of Hansikucho, Blackjumma, Sanshinja, Gosam, and White Sash.

Also, the herbal medicine composite extract of the present invention provides the herbal medicinal compound extract containing health and ginseng functional ingredients in addition to the above-mentioned mixed ingredients.

The herbal composition complex extract of the present invention is obtained by extracting the above components with at least one solvent selected from the group consisting of water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane, The production of the herbal medicine composite extract by using hot water, ethanol, methanol, and buteneglycol, respectively, in accordance with the solvent, but the present invention is not limited thereto. However, depending on the solvent used, it affects the proliferation of the dermal papilla cells, which play a key role in hair growth. Most preferably, the herbal medicine complex extract extracted with ethanol produced in Example 2 activates the papillary cells and promotes the proliferation of the papillary cells.

More preferably, in the herbal medicine composite extract of the present invention, the preferred solvent is extracted under 50 to 90% ethanol, and most preferably extracted under 70% ethanol.

Hereinafter, each composition selected as the herbal medicine for preventing hair loss and promoting hair growth of the present invention will be described.

The medicinal herb of the present invention is limited to the composition in which the effect of preventing hair loss and promoting hair growth is confirmed through an evaluation test for promoting proliferation of hair follicle cells (Hair Follicle Dermal Papilla Cell).

In addition, the herbal medicine used in the present invention is similar to the Korean Food and Drug Administration Notice No. 2012-110 " Criteria and Test Method for Residual Contaminants such as Herb Medicine " (Korea Food and Drug Administration Notice No. 2011-42, Aug. 22, 2011) Use only medicines that have passed the test.

As a component of the herbal medicinal compound extract of the present invention, the scientific name of Hansencho is Eclipta Prostrata L and is an annual herbaceous plant belonging to Asteraceae. It grows in grasses and fields around the ditches of central and southern Korea, relatively humid. The quality is cold and the taste has a sweet and sour taste. Kidneys, and meridians of the liver. It is said that the flavor of Hanwookcho is sweet and it has the effect of hemostasis. It makes beard, makes hair black, treats dysentery and diarrhea. In addition, Dong-bo-gyo said, "Hansikcho has long hair and dark gray hair. It is collected in June, and ginger juice and honey are put into it and cooked. Take a spoonful of hyeolgokgo, hanryikgo, etc.) 膏) 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 을 It is dried in the sun for 5 days and then mixed with ginger juice and good honey each month and dried in the sun for a few days to make it like a thin spoon. It is stored in a porcelain bowl and eats a glass of spoon and about a spoon early in the morning, I also eat it. After 21 days, if you pull out your white hair, you will get black hair from that hole. "

Black bean (black bean) is a scientific name Sesamum indicum is a black seed of an annual plant with sesame seeds. It is likely that the origin of sesame seeds originated in Africa and that it originated in India but spread to Persia, Mesopotamia, Asia Minor, and Egypt and spread to Europe. He assumed that he came to China through an Arabian merchant, and that he was brought from China to China. Black jersey is a healthy kidney and has the effect of replenishing blood to blacken the hair and prevent the hair from falling out of old diseases. Black marbles are harvested from August to September and dried. It is cultivated in each farmhouse in Korea. Temperatures are not hot or cold, but taste is sweet. It affects the kidneys, liver, and meridians of the large intestine. It is said that "black bean tastes sweet and swollen (swollen), it is supplemented with weakness and strengthens strength". In addition, Dongwoo-bo feel: "When you put oil on black scalp and apply it on scalp, hair grows in baldness. It is steamed 9 times and it is dried in the sun, then powder is taken out and made into circle. If you boil and close your hair, hair will be lengthened.It's black and has a good taste and sweet taste.It replenishes your energy, makes your muscles healthy, makes your bones hard, makes your five lustrous, replenishes your bone marrow, Substances that are fundamental to the generation of spermatozoa and their activities), extend their life span and prevent them from aging. "

Heartbreaker is a scientific name Morus alba L. Mulberry fruit or dried medicinal material. It is widely called "Oddi", and the lonely person has started to use medicine since Tang Dynasty. The medicine is mild and the taste is sweet. Vitamin A (A B1 B2 D), calcium, phosphorus and iron are contained in glucose and fructose, citric acid, malic acid, tannin, and pectin. The quality is cold and the taste has a sweet and sour taste. It affects kidneys, liver, and heart meridians. It is said that "the taste of the loner is sweet, it treats the noise (diabetes), he heals the stone poison (poison) and darkens the hair". In addition, Dongwoo-bo said, "The nature of the loner is cold and the taste is sweet." It is good to treat the noise and to benefit the longevity, and do not feel hunger when taking it for a long time. ) And hang it on the east side of the house, and when it becomes a hundred days, it becomes like black mud, and when it is dyed with white hair, it becomes black. The bark of mulberry root causes swelling, sputum, thirst The mulberry leaves will remove the edema and benefit the small intestine The mulberry tree treats the cough, indigestion, and masturbation The mulberry blossom treats the symptoms of puffing blood and the fever. "

Gosam (苦 蔘) in Korea, China, and Japan both use the root of the gosam ( Sophora flavescens Aiton ) almost peeled. The name Gosam uses the word "苦" for taste and the word "參" for similarity of efficacy. Because of the appearance of the roots, it has a plant name of a thief's cane. Since ancient times, the strong anti-allergic and antibacterial herb Gosam has been used as a remedy for various dermatoses such as acne, eczema, and acne in a herb using dried root. In 2004, the International Journal of Dermatology Dermatological Science ' Journal of the Korean Society for Thoracic and Cardiovascular Surgery. Since the extract has a function of strongly inhibiting the enzyme called 5-alpha-reductase, which is a direct cause of male hair loss, it has been reported that when male ghrelin is mixed with ghoshum extract, active male hormone which causes hair loss is greatly reduced. In addition, the extract of Gosam showed not only inhibition of hair loss but also the action of promoting hair growth. Since the extract of Gosam influences keratinocyte closely related to hair growth, It was reported that the effect of hair growth when applied to the extract.

There are two kinds of red and white white shochu (white shochu), of which white shochu refers to. Asclepiadaceae Large mockery ( Cynanchum wilfordii Hemsley ) and the name of herbal medicine is Baesuo Oh. The quality of the seaweed is warm and the taste has bitter taste, sweetness, sweet taste and acts on the meridians of the liver and kidney. Donguibogam said, 'Sasuo replenishes tiles and blood, keeps muscles and bones healthy, fills bone marrow, darkens hair, pleases the face, and prolongs its life. His real name is Kagoshima (夜 交 藤), the name was taken by a man named Hashuro. The vines of the mulberry are purplish, flowers are yellowish white, and the leaves are like horses. There are two kinds of leaves: glossy and noisy, roots fisted, reddish male, white female. It is the treasure that the shape of the roots looks like a mountain. "

In addition to the above-mentioned mixed components, health and ginseng may be further contained as a functional ingredient.

At this time, the health is dried root of ginger (Zingiber officinale Roscoe). The Hindu Danang area of the East India is presumed to be the country of origin and it is said that it was cultivated 2,500 years ago in China. Now Sichuan province is known as the origin of ginger. In Korea, ginger cultivation was recorded for the first time in Goryeo (9th year of King Hyeongjong, 1018), and it was recorded that ginger was written as a royal slave. It has long been used as a food spice and has been used in herbal medicines. The spicy taste of ginger contains ginger, shogaru, kilgibe, kilgirren and chitral. Ginger is warm in nature, but the shell has a cold nature, so use it to remove the skin when you warm it up, and use it with cold skin when you use it. Recently, hair loss caused by stress and tension in the scalp due to blood flow is said to be the main cause. If you do not have enough water or lack of fertility when you grow rice in a rice field, rice will not grow well. If you can not feed your scalp with blood flow well, you will lose nutrition of your hair and get rid of it easily. Therefore, in recent years, blood flow to the scalp to improve the supply of blood is much better treatment. The ginger eliminates airflow, coldness, and humidity and improves the circulation of the blood, thus providing a good effect on hair loss.

Ginseng is a perennial herbaceous plant belonging to Araliaceae and its scientific name is Panax schinseng NEES. Root is medicinal, and its form is human, so it is called ginseng. In order to distinguish ginseng from other herbs called ginseng, Korean ginseng is called "Korean ginseng" and "ginseng". Foreign ginseng is written as "參". In Korean literature, the oldest recorded ginseng in the «Samguksagi» or «Emergency Clinic for Emperor 鄕 药» is the oldest. The efficacy and efficacy of ginseng has long been studied. From ancient times, ginseng has been referred to as flour, jangsu, 益气 (益气) It is also known that it has various effects and effects such as skin cell regeneration and wrinkle improvement as cosmetic raw materials, and it supplies nutrition to scalp and hair, gives shine to hair, prevents hair loss, It is known to have an effect.

In the above-mentioned herbal medicine composite extract, the mixed ingredients including Hansikucho, black jam, ssamsei, gosam and white sachet are mixed in the same weight ratio between the respective ingredients, and the additional functional ingredients are mixed in the same weight By weight, preferably 0.5 to 1 part by weight based on the weight of the mixed components.

In addition, the present invention relates to a method for producing a polyvinyl alcohol solution, which comprises the steps of: 1) mixing the ingredients in the same weight ratio between the ingredients, and mixing the ingredients in water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane And hexane to obtain a crude extract,

2) The crude extract is filtered and purified by any one method selected from filter cloth, filter paper and vacuum filtration apparatus,

3) A method for producing a herbal medicine composite extract having the effect of preventing hair loss and promoting hair growth, including freeze-drying.

Among the manufacturing methods of the present invention, hair growth inhibition and hair growth promoting ability of promoting the proliferation promotion in the hair follicle dermal papilla cell (Hwangsikcho, black jima, As a proven effective ingredient, it is the same as that described in the herbal medicine composite extract, so it is omitted.

And the extract is preferably extracted with shaking at 50 to 120 DEG C for 2 to 24 hours. The extraction is preferably performed at a temperature of 50 to 120 DEG C for 2 to 24 hours.

Thereafter, among the production methods of the present invention, the freeze-drying in step 3) is carried out from -20 ° C to 30 ° C stepwise for 24 to 72 hours to obtain a dry powder.

Further, the present invention provides a composition for hair growth comprising 0.001 to 30% by weight of a herbal medicine composite extract having hair loss prevention and hair growth promoting effect, which is useful for hair loss prevention and hair growth promotion.

In this case, the content of the herbal medicine composite extract is more preferably 0.05 to 2.00% by weight. When the content is less than 0.001% by weight, it is difficult to obtain antioxidant and hair growth promoting effect. It is difficult to expect an increase in efficacy.

1: 1: 1: 0.5: 0.5 weight ratio of the mixed ingredients of the herbal medicine extract of the present invention, more preferably the herbal medicine, black jima, (Hair Follicle Dermal Papilla Cell) to increase the promotion of proliferation of the papilla cell, it is possible to provide a composition for hair growth which is useful for preventing hair loss and promoting hair growth have.

FIG. 1 is a graph showing the hair growth promoting experiment using the herbal medicine complex extract extracted from ethanol of the present invention. As shown in FIG.

In the examples of the present invention, a hair tonic, a hair shampoo and a hair conditioner are described as preferred formulations for the composition for hair growth. However, the present invention is not limited to the formulation of known hair products such as hair essence and hair cream.

Hereinafter, the present invention will be described in more detail with reference to Examples.

The present invention is intended to more specifically illustrate the present invention, and the scope of the present invention is not limited to these embodiments.

< Example  1> In hydrothermal  Of herbal medicinal compound extract

1200 g of distilled water was added to 120 g of a mixture of 20 g of dried Hansikucho, 20 g of black bean curd, 20 g of fresh ginseng, 20 g of red ginseng, 20 g of white ginseng, 10 g of healthy ginseng and 10 g of ginseng, boiled for 2 hours in a hot water extractor equipped with a cooling condenser, After cooling, the medicament was filtered with a 380-mesh filter cloth and filtered through a 11 탆 paper filter. A portion of the filtrate was filtered through a 0.45 μm membrane filter under reduced pressure, and then lyophilized to obtain a final herbal medicine composite extract.

< Example  2> Production of herbal medicine complex extract by ethanol

60 g of 70% ethanol was added to 60 g of a mixture of 10 g of dried hanjiccho, 10 g of black bean curd, 10 g of red ginseng, 10 g of red ginseng, 10 g of white algae, 5 g of healthy ginseng and 5 g of ginseng.

After extraction, the medicinal material was filtered with a 380 mesh filter cloth and filtered through a 11 mu m paper filter. The entire filtrate was filtered through a 0.45 μm membrane filter under reduced pressure, and then concentrated under reduced pressure. The final herbal medicine composite extract was lyophilized.

< Example  3> Production of herbal medicine complex extract by methanol

100 g of 99% methanol was added to 60 g of a mixture of 10 g of dried Hansikucho, 10 g of black currant, 10 g of ginseng, 10 g of ginseng, 10 g of bagasse, 5 g of health, and 5 g of ginseng.

After extraction, the medicinal material was filtered with a 380 mesh filter cloth and filtered through a 11 mu m paper filter. The entire filtrate was filtered through a 0.45 μm membrane filter under reduced pressure, and then concentrated under reduced pressure. The final herbal medicine composite extract was lyophilized.

< Example  4> To butylene glycol  Of herbal medicine complex extract

1900 g of 30% butylene glycol (1:20 ratio wt / wt ) was added to 95 g of a mixture of 15 g of dried Hansikcho, 15 g of black bean curd, 15 g of fresh curd, 15 g of ginseng, 15 g of white algae, 10 g of health and 10 g of ginseng. 3 days. After extraction, the medicinal material was filtered with a 380 mesh filter cloth and filtered through a 11 mu m paper filter. The whole filtrate was filtered through a 0.45 μm membrane filter under a reduced pressure filtration apparatus to obtain a final herbal medicine composite extract.

< Example  5> Production of herbal medicine complex extract by ethanol

500 g of 70% ethanol was added to 50 g of a mixture of 10 g of dried Hansikucho, 10 g of black bean curd, 10 g of fresh curd, 10 g of ginseng, and 10 g of white algae, and the mixture was subjected to third round extraction at room temperature for 3 days.

After extraction, the medicinal material was filtered with a 380 mesh filter cloth and filtered through a 11 mu m paper filter. The entire filtrate was filtered through a 0.45 μm membrane filter under reduced pressure, and then concentrated under reduced pressure. The final herbal medicine composite extract was lyophilized.

< Experimental Example  1> Dermal papilla cells  Splitting propagation experiment

Step 1: Dermal papilla cells  culture

HFDPC (Hair Follicle Dermal Papilla Cell) was cultured in Hair Follicle Dermal Papilla Cell Growth Media (supplement mix, 1X Antibiotic-Antimycotic). The HFDPC was seeded on a T75 plate at 2 × 10 ⁵ cells and cultured for 4-5 days. Supplementary free media was then fed to a 48-well plate at 6000 cells / well, Respectively.

Step 2: Extract treatment

After the cells were cultured and fixed in all wells, the growth medium was removed, and the herbal medicine complex extract was diluted to the respective concentrations in supplement-free media. Then, (Supplement-), (2) 50 μg / ml, (3) 100 μg / ml, (4) 200 μg / ml and (5) control group (supplement +).

After 24 hours and 48 hours intervals, the sample medium of each well was changed and the degree of cell division proliferation was measured 48 hours and 72 hours after the first herbal medicine composite extract treatment.

Step 3: Dermal papilla cells  Measure proliferation proliferation

EZ-Cytox is a cell viability reaction kit using high sensitive water soluble terazolium salt (WST). It is widely used as a method to more accurately reflect the cell state by reacting with all the dehydrogenase in the cell. The medium for each well was removed and replaced with supplement-free media (200 μl / well volume for 48 wells). The wells were treated with 20 占 퐇 / well of Assay reagent provided by EZ-Cytox and cultured in a 5% CO ₂ incubator at 37 占 폚 for 2 hours. The absorbance was measured at 450 nm and the data were collected and the number of cells was calculated based on the OD ratio.

The results of the experiments for the proliferation of dermal papilla cells using the herbal medicine composite extract obtained from the hot water in Example 1 are shown in Table 1 below.

As shown in the above Table 1, after 3 days of the treatment with the herbal medicine, the herbal medicine complex extract by hot water, compared with the negative control (supplement -), contained 50 μg / ml 86.16%, 100 μg / ml 82.79%, 200 μg / And 87.41%, respectively, and the results showed that the demineralization of dermal papilla cells was decreased.

Table 2 shows the results of experiments on the proliferation of dermal papilla cells using the herbal medicine composite extract obtained under ethanol in Example 2 above.

From the results of the above Table 2, it was found that the extract of herbal medicine complex obtained under ethanol had 24.44%, 43.19% and 200 .mu.g / ml in the case of 50 .mu.g / ㎖, and 32.69%, respectively. That is, the herbal medicine complex extract obtained under the ethanol produced in Example 2 activates the dividing dermal papilla cell proliferation, and thus it is confirmed that hair loss prevention and hair growth promoting effect are excellent.

Table 3 shows the results of experiments for proliferation of dermal papilla cells using the herbal medicine composite extract obtained under methanol in Example 3 above.

From the results shown in the above Table 3, it was found that the herbal medicine composite extract obtained under methanol had a concentration of 50 μg / ml 84.28%, 100 μg / ml 87.87%, 200 μg / ml 87.57% And the results are shown in Fig.

Table 4 shows the result of the experiment for the proliferation of dermal papilla cells using the herbal medicine composite extract obtained under butylene glycol in Example 4 above.

From the results in Table 4, it was confirmed that the herbal medicine complex extract obtained under butylene glycol showed an insignificant increase of 50 μg / ml 2.60% and 100 μg / ml 1.56% after 3 days of the extract treatment compared to the negative control , And the result was that the demineralization of the dermal papilla cells was reduced to 200 μg / ml 90.83%.

Table 5 shows the results of the experiment for the development of the dermal papilla cell proliferation using the herbal medicinal compound extract containing the mixed ingredients of Hanshikoshi, Blackjima, Sanshinja, Gosam, and White Seaweed extracted by ethanol in Example 5 above.

From the results shown in the above Table 5, it can be seen that the herbal medicinal compound extract containing the mixed ingredients of the extracts of Hanshikoshi, Blackjima, Sanshinja, Gosam and White Sash were extracted from ethanol by 3 days after the treatment with the extract , 10.85% for 50 μg / ml, 20.06% for 100 μg / ml and 13.21% for 200 μg / ml, respectively.

< Experimental Example  2> Promotion of mouse hair growth

C57 / BL6 mice were used to measure hair growth and improvement effects. 15 herbal medicinal compound extracts obtained under ethanol produced in Example 2 and ethanol as a negative control group, which were the most effective among the results of the proliferation test of dermal papilla cells, were injected into 15 mice.

The C57 / BL6 mouse used for the measurement was a 6-week-old 6-week-old resting hairs whose pink color appeared on the back skin. Carefully, the hair of the rats was shaved off, As a negative control, 0.2 ml of ethanol was applied to each sample. The sample was treated twice a day for two weeks with the mouse's hair growing on its back completely covered. At this time, hair growth was observed at 4 days, 7 days, 10 days and 14 days after the sample treatment.

The results are shown in FIG. 1 , and when the herbal medicine complex extract prepared under the ethanol prepared in Example 2 was administered at 14 days after application of the sample, the hair growth effect was excellent Respectively.

< Example  6> Manufacture of Cosmetic Formulation

A variety of cosmetic formulations containing the herbal medicine complex extract obtained under ethanol prepared in Example 2, which has the best proliferation effect on dermal papilla cells, were prepared.

Prescription Example 1: Manufacture of hair tonic

The hair tonic of Formulation Example 1 composed of the raw materials shown in Table 6 was prepared.

Prescription Example 2: Manufacture of hair shampoo

The hair shampoo of Formulation Example 2 composed of the raw materials shown in Table 7 below was prepared.

Prescription Example 3: Manufacture of hair conditioner

The hair conditioner of Formulation Example 3 composed of the raw materials shown in Table 8 below was prepared.

The various cosmetic formulations prepared in Tables 6 to 8 contain the herbal medicinal compound extract obtained under ethanol produced in Example 2 having the best effect of enhancing the apical cell division proliferation among the herbal medicine composite extracts of the present invention, In the same manner as in Example 1. As a result, it was confirmed that the hair follicle proliferation prevention effect and hair growth promoting effect can be confirmed by activating the proliferation of the epithelium in the scalp.

As described above, the present invention provides a herbal medicine composite extract which has been proved to be effective in preventing hair loss and promoting hair growth by selecting from herbaceous herbal medicine which has been proven to be stable and evaluating ability of promoting proliferation promotion in dermal papilla cells.

The herbal medicinal compound extract of the present invention is a herbal medicine herbal compound extract containing mixed ingredients of the herbaceous herb, black jam, spermicide, ginseng and white seaweed, or the mixed ingredient further contains health and functional ingredients of ginseng, The present invention provides a cosmetic composition for hair growth containing the active ingredient as an active ingredient.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (11)

An extract of an herbal medicine compound having an effect of preventing hair loss and promoting hair growth, which contains ethanol extract of 50 to 200 占 퐂 / ml extracted at the same weight ratio between mixed ingredients of Hansikucho, blackjack, sickle, The herbal medicine composite extract according to claim 1, wherein the mixed ingredient further contains health and ginseng components in a ratio of 0.5 weight of any one of the mixed ingredients. delete delete delete The mixture was mixed at the same weight ratio between the components of the mixture of Hansuksikcho, blackjack, sickle, gosam and white sachet, and extracted into an ethanol extraction solvent to obtain a crude extract,
The crude extract is filtered and purified by any one method selected from filter cloth, filter paper and vacuum filtration apparatus,
A method for producing a herbal medicine composite extract having the effect of preventing hair loss and promoting hair growth according to claim 1, which comprises lyophilization. 7. The method according to claim 6, wherein the mixed ingredient is further mixed with a health and ginseng ingredient in a ratio of 0.5 weight of any one of the mixed ingredients. Way. [Claim 7] The method according to claim 6, wherein the extraction is shaken at 50-120 [deg.] C for 2 hours to 24 hours. The method according to claim 6, wherein the freeze-drying is performed from -20 ° C to 30 ° C for 24 to 72 hours to obtain a dry powder. Way. The present invention relates to a hair treatment composition comprising 0.001 to 30% by weight of an extract of a herbal medicine complex which is an ethanol extract of 1: 1: 1: 1: 1 by weight of the mixed ingredients of the ingredients 1, 1, 1, A cosmetic composition for hair growth useful for promotion. The herbal medicinal compound extract of ethanol extract of 1: 1: 1: 1: 1: 0.5: 0.5 ratio between the ingredients of the second item of the present invention in a mixture of the ingredients of Hansikcho, Blackjack, % Of the hair growth inhibitory effect of the present invention.

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