KR20170137465A - Composition for improving skin condition comprising herb extracts mixture - Google Patents
Composition for improving skin condition comprising herb extracts mixture Download PDFInfo
- Publication number
- KR20170137465A KR20170137465A KR1020160069638A KR20160069638A KR20170137465A KR 20170137465 A KR20170137465 A KR 20170137465A KR 1020160069638 A KR1020160069638 A KR 1020160069638A KR 20160069638 A KR20160069638 A KR 20160069638A KR 20170137465 A KR20170137465 A KR 20170137465A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- skin
- roots
- present
- ginseng
- Prior art date
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-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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Abstract
Description
The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.
The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.
Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in its three-dimensional structure by the degrading enzyme elastase, which is activated after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.
When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.
When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.
In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.
A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H 2 O 2 ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.
Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.
Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.
In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.
Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.
A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.
In order to solve the above-mentioned problems, the present invention provides a method for producing a ginseng extract, a ginseng extract, a ginger extract, a ginseng extract, a ginseng extract, a ginseng extract, a ginseng extract, a ginseng extract, And a composition for skin whitening, wrinkling, elasticity, trouble, or antioxidation (for example, a cosmetic composition) containing the extract as an active ingredient.
[extract]
Gastrodia Rhizome is a gastrodia elata Blume (Orchidaceae Orchidaceae) is steamed and dried. It is slightly curved and flat-pressed, cylindrical to fusiform, 3-15 cm long, 1.5-5 cm wide and 0.5-2 cm thick. There is a peculiar smell and the taste is sweet. Chimma is divided into wild chimma and cultivated chimma. Wild chunks are usually divided into cannon spring (spring 麻) in spring and cannon (冬 麻) in winter. Chimma acts on the liver and is used for seizures, tetanus, tidal velocities, dizziness, headache, nervous breakdown, and headache. The pharmacological effects have been reported to be sedative, anticonvulsant, analgesic, anti-inflammatory, increased heart and cerebral blood flow, hypotension, increased antioxidant activity, and immune activation.
Pueraria Root (葛根, Pueraria Root) 칡 Pueraria lobata Ohwi (leguminosae legumes) as it is the root or junk is removed. It is very tasty, and its energy is flat and it is neither hot nor cold. The outer shape is finely cut in hexahedron or plate-like shape, and the outer side is gray or yellow or white. The transverse cut is made of a fibrous neck and a starch-like soft tissue that are staggered to form a vertical pattern. It is easy to crack vertically and the cracked surface has a lot of fiber. It treats cold, high fever, headache, muscular pain, stiffness of the neck.
Viticis Fructus is a vine plant of Vitex rotundifolia Linne elephant. Or manhyeong (蔓荊) Vitex It is a ripe fruit of trifolia Linne (Verbenaceae). Fruit appearance is round or oval shape, outer side is black or black brown, and ⅓ to ⅔ from lower side is wrapped with thin calyx with grayish white color. When you look at the cross section of this medicine with a magnifying glass, the fruit is divided into four pieces and one white small seed is contained in it. There is a peculiar direction and the taste is sweet and slightly spicy. The pharmacological effects of sedation, pain relief, fever, and visceral circulation were reported.
Baekjaek (白 杏药) is a peony Paeonia It is the root of lactiflora Pallas or other related plants (Paeoniaceae and Paeoniaceae). It grows in deep mountains. It is 40 ~ 50cm in height. The roots are thick and fleshy, and the bottom is covered with scaly leaves. Leaves are 3 ~ 4 slices and split 3 ~ 2 times. The small leaf is long oval or the egg is turned upside down. The edge is flat and has no hair. The flowers bloom in June and are 4 ~ 5cm in diameter and run one at the end of the main stem. Calyx is ovate, 3, petals are inverted, 5 ~ 7. There are several operations and three or four ovaries. Fruits are red and undersized red seeds and mature black seeds. It has a hairy back on the back of the leaf (var.pilosa), a hair on the back of the leaf, a long style flower that grows backward and has a red flower (P.obovata) (povar.glabra). Roots are used for analgesia, gynecology, and women's diseases. It is distributed in Korea, Japan, China, Sakhalin islands.
Windshield (Saposhnikovia Root) is Saposhnikovia It is the root of divaricata Schischkin (mountain type and Umbelliferae). The roots are elongated, conical, tapering downward, 15 to 20 cm in length, and 7 to 15 mm in diameter. The outer surface is light brown, has many vertical wrinkles, and has fine roots. The body is light and the vagina is soft and easy to cut. The cut face has light brown skin, several openings, and the neck is light yellow. There is a peculiar smell and the taste is slightly sweet. It grows in dry sandy grass. It is about 1m in height. Branches are divided a lot. The seeds bloom in three years after budding. Many leaves come from roots. The leaves on the stem are alternate, petioles become shorter as they go upwards, and the lower part becomes sheaths and is divided into three horns. The split pieces are in the shape of a line, with sharp ends and a little stiffness. The flower blooms in July to August in white and runs on a stem and a branch with a double-bladed inflorescence. Small guns are small and 5-6. Ovary is subordinate. Fruit is split and flat, wide elliptical, and there are protrusions when young. Northeast of China, Huay Bay, and Inner Mongolia.
Ostericum Root is an Ostericum koreanum roots of Maximowicz or Chinese ganghwu Notopterygium incisum Ting or 寬 葉 羌 活Notopterygium forbesii Roots and roots of Boissier (mountain and Umbelliferae). It is prescribed to cold, headache, neuralgia, rheumatism, arthritis, paralysis. It is distributed in Korea (Gyeongbuk, Gangwon, Gyeonggi, Pyongbuk, Hamnam, Hambuk), and northeast of China. The roots of Ganghwam are conical or long conical, usually branched, 15 ~ 30 cm long, 2 ~ 5 cm in diameter. The outer surface is yellowish brown to brown, and there are beard roots or marks on it. There is a peculiar smell, the taste is sweet at first and cool afterwards and a little bit later. The roots and rootstocks of Chinese ganghwam are slightly curved, sometimes branched, 4 ~ 13 cm long and 6 ~ 25 mm in diameter. There is a fragrance and the taste is a little spicy. The roots or rootstocks of the houseplant Ganghwam are close to the cylindrical shape, and the stalks of the stem and leaf sheath remain at the upper end. Its roots are conical, with vertical wrinkles and peel eyes. There is a slight smell, and the taste is relatively cool.
Aralia cordata (獨活, Aralia Continentalis Root) is Aralia cordata Aralia It is the root of the continentalis Kitagawa (Araliaceae and Araliaceae). It grows in the mountains. The height is 1.5m and there is a little hair on the whole except flowers. Leaves are alternate phyllotaxis, length 50 ~ 100cm, butterfly 3 ~ 20cm, odd number of double haploid leaves, and light brown hairs when they are young. The small leaf is ovate or oval with sawteeth on the edge. The surface of the leaf is green, the back is white, and there is a small cotyledon on both sides of the petiole. Flower is bloomed from July to August with a large, light green bloom, conical flower growing, and blooming with a mountain-shaped inflorescence at the tip of a branch separated by a gun shape. Fruits are berries and ripen black in September-October. Roots are often used for muscle aches, paraplegic, headache, paralysis of paralysis. It is distributed in the mountains of East Asia.
Licorice is the Latin name Glycyrrhizae Radix et Rhizoma, Licorice, Glycyrrhiza uralensis Fischer, Glycyrrhia za glabra Linne or licorice and licorice Glycyrrhiza inflata Batalin (leguminosae) roots and / or rootstock as it is (ie, with the bark attached) or removed from the juniper. It has a peculiar smell and taste is sweet. Jinhae, geomung, muscle relaxation, diuretic action.
Ginseng (ginseng) is the root of Panax ginseng CA Meyer (Araliaceae and Araliaceae), or as it removes thin roots and cork layers. Pharmacological actions have been reported in cerebral cortex excitation and inhibition, equilibrium, anti-fatigue, anti-aging, immune enhancement, cardiac contraction, gonadal stimulation, hyperglycemia inhibition, protein synthesis promotion, homeostasis, anticancer and detoxification. Appearance is elongated columnar or fusiform type with 2 ~ 5 side roots and outer surface is light tan, with vertical wrinkles and thin root marks. Muscle head has an outline with a stem residue attached. In Korea, white ginseng (raw ginseng), red ginseng (red ginseng: steamed ginseng) and ginseng (蔘 蔘: thin roots) were recorded separately. In the private sector, however, they are distinguished from wild ginseng camphor and wild ginseng.
Shiho (Bupleurum Root) is the root of Bupleurum falcatum Linne or its variants (haploid and Umbelliferae). The roots are elongated cones or cylinders, single or split, 10-20 cm long, 5-15 mm in diameter. They are found in Korea, Japan, China, Mongolia, Siberia, Caucasus, Europe and live in grasses. There are no hairs in the whole aeration, and the branches are divided on the thin long stem. Roots are thick and short. The leaves are staggered, line-shaped or bar-shaped, the edges are flat, and the veins are parallel. The leaves on the roots are like petiole with the lower part narrowed and 10 ~ 30cm long. The leaves on the stem are 4 ~ 10cm long, 5 ~ 15mm long and the tip is pointed. Flowers bloom from August to September in yellow, with a double-inflorescence at the end of stem and branch. There are 2 ~ 7 peduncle, 5 ~ 10 flowers each. The gun flakes are narrow, narrow, split into 5 segments, 15 mm long, and the small guns are long elliptical or wider, 2.5-4 mm long. There are 5 petals and stamen and the ovary is lower (lower). Fruit is elliptical with division, about 3mm in length, ripened in September-October. Reproduction is either giving up or giving up seeds.
Cyperus Rhizome is a herb Cyperus Rotundus Linne (Chrysanthemum and Cyperaceae), the roots of the thin roots are removed. The brown and fur-like fibers in the nodules are not removed, and they are called curls. The removed ones are called incense rice, and they are called fragments. Rootstock mainly spindle type, length 1.5 ~ 3.5cm, diameter 0.5 ~ 1cm. Outer surface is brown to dark brown, has vertical wrinkle pattern, and stem is left on top. In addition, there are five to ten uneven ring nodes protruding, and there are traces of brown hairy roots and beard roots on the nodes of unfinished ones. The removal of the hairy roots in advance is relatively smooth, and the rings are not clear. Quality is hard. Roasted or boiled surface is yellowish brown to reddish brown and keratinized, and the dried surface is white and the powdery characteristic is clear. The endothelial layer is distinctive in its ring pattern, the color of the central region is relatively thick, and the vascular vasculature is scattered. There is a distinctive scent and taste is a little bit.
Ginger (ginger) is a fresh roots of Zingiber officinale Roscoe (ginger and Zingiberaceae). Southeast Asia is the origin and is grown as vegetables. The rootstock grows sideways and is fleshy, lumpy, yellowish, with a pungent flavor and a fragrant smell. At each node of the rootstock, a fake stem made of a sheath is straight up to a height of 30 to 50 cm, and the leaves are arranged in two rows at the top. Leaves are slanted, bar-shaped, with narrow ends on both ends and long bottom leaves. Pharmacological action to promote gastric secretion, digestive power, cardiac excitement, blood circulation promotion, and bacillary action have been reported.
Chengdu (川芎) is a Cnidium officinale Makino, China Taipei (中國 川芎) Ligusticum chuanxiong Hort. (Hill and Umbelliferae), a perennial herbaceous plant belonging to the mountain type. It is a Chinese plant and it is commonly cultivated in Korea. It has been reported that it has mildness, tastiness, soothing effect and hematopoiesis. In addition to raw materials, it is often taken in water.
Contrast (Jujube is the jujube tree Zizyphus There is JuJuba Miller. inermis Rehder or Bojang Jujube Trees Zizyphus There is JuJuba Miller. It is a ripe fruit of hoonensis TB Lee (Gramineae Rhamnaceae). Jujube Zizyphus There is JuJuba Miller. The inermis Rehder is Zizyphus Includes jujuba Miller. Fruits are elliptical or spherical, 2 to 3 cm long, and 1 to 2 cm in diameter. The outer surface is reddish brown to dark red with a fine wrinkle and shine. Both ends are slightly concave, with one end bearing a style and the other end bearing a fur seal. Surgical blood is thin and tough like a leather. The cecum is thick, dark gray brown, and is spongy, soft and sticky. Internal medicine The blood is hard fusiform, one end is pointed, the inside is divided into two spaces, the flat oval seed is contained and the quality is hard. There is a peculiar smell and the taste is sweet. The jujube jujube is almost elliptical compared to the fruit of the jujube, and the seed has almost no 仁.
The present inventors have found that all of the above extracts have a biological activity beneficial to the skin, and that the extracts of two or more of the above extracts, preferably Chimmae extract, Puerariae Radix extract, Panthera extract, , Ginseng Extract, Seoho Extract, Herbal Extract, Ginger Extract, Astragalus Extract, and Control Extract have a synergistic biological activity.
In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.
Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.
In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.
The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.
The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.
In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.
[Composition]
The composition according to the present invention is effective for extracting ginseng root extract, ginseng extract, ginseng extract, betel extract, windproof extract, ganghyeol extract, ginger extract, licorice extract, ginseng extract, ginseng extract, herbal extract, ginger extract, As an ingredient.
In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.
Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of Ganoderma lucidum extract, Puerariae Radix Extract, Gentian Extract, Vicinity Extract, Windproof Extract, Ganghyeum Extract, Fragrant Extract, Licorice Extract, Ginseng Extract, Seiho Extract, Herbal Extract, Ginger Extract, : 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.
The composition according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010% 0.0021%, 0.002%, 0.002%, 0.002%, 0.0024%, 0.0025%, 0.002%, 0.002% , 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% %, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059% 0.0071%, 0.007%, 0.007%, 0.007%, 0.007%, 0.005%, 0.006%, 0.007% , 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092% %, 0.0095%, 0.0096%, 0.0097%, 0.0 0.025%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.005%, 0.08%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300% , 0.075%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950% %, 0.1000, 0.1250, 0.1500, 0.1750, 0.2000, 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250, 0.6500, 0.6750, 0.7000, 0.7250, 0.7500, 0.7750, 0.8000, 0.8250, 0.8500, 0.8750, , 0.9200%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2% , 2.4%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7% 4.5%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5% , 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1% 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 7%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9% , 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14% %, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% The composition may comprise one or more extracts of the present invention in the range of 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99%
The composition according to the present invention may also contain at least about 0.0001% to 90%, 0.001% to 90%, 0.01% to 90%, 0.1% to 90%, 0.1% to 90% %, 0.1 to 80%, 0.1 to 70%, 0.1 to 60%, 0.1 to 50%, 0.1 to 40%, 0.1 to 30%, 0.1 to 20% or 0.1 to 10% . ≪ / RTI >
The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.
The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.
The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.
The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.
The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.
In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .
The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.
May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.
The composition according to the present invention may be provided as a topical skin composition.
In addition, a method of topically applying the composition according to the present invention to the skin can be provided.
In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.
[Skin improvement effect]
The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.
More specifically, the composition according to the present invention has a skin whitening effect.
The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.
As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.
In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.
The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.
The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.
The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .
As a result of treating the complex extract according to the present invention, the present inventors confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And the effect of inhibiting the activity of skin elastase was shown to exert an effect corresponding to the known quercetin. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkles and elasticity improving effect.
Further, the composition according to the present invention has an effect of improving the trouble.
The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.
The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.
As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.
In addition, the composition according to the present invention has an antioxidative effect.
The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.
As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.
In addition, the composition according to the present invention has an antiherating effect.
The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.
As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.
In order to solve the above-mentioned problems, the present invention provides a method for producing a ginseng extract, a ginseng extract, a ginger extract, a ginseng extract, a ginseng extract, a ginseng extract, a ginseng extract, a ginseng extract, And a composition for skin whitening, wrinkling, elasticity, trouble, or antioxidation (for example, a cosmetic composition) containing the extract as an active ingredient.
[extract]
Gastrodia Rhizome is a gastrodia elata Blume (Orchidaceae Orchidaceae) is steamed and dried. It is slightly curved and flat-pressed, cylindrical to fusiform, 3-15 cm long, 1.5-5 cm wide and 0.5-2 cm thick. There is a peculiar smell and the taste is sweet. Chimma is divided into wild chimma and cultivated chimma. Wild chunks are usually divided into cannon spring (spring 麻) in spring and cannon (冬 麻) in winter. Chimma acts on the liver and is used for seizures, tetanus, tidal velocities, dizziness, headache, nervous breakdown, and headache. The pharmacological effects have been reported to be sedative, anticonvulsant, analgesic, anti-inflammatory, increased heart and cerebral blood flow, hypotension, increased antioxidant activity, and immune activation.
Pueraria Root (葛根, Pueraria Root) 칡 Pueraria lobata Ohwi (leguminosae legumes) as it is the root or junk is removed. It is very tasty, and its energy is flat and it is neither hot nor cold. The outer shape is finely cut in hexahedron or plate-like shape, and the outer side is gray or yellow or white. The transverse cut is made of a fibrous neck and a starch-like soft tissue that are staggered to form a vertical pattern. It is easy to crack vertically and the cracked surface has a lot of fiber. It treats cold, high fever, headache, muscular pain, stiffness of the neck.
Viticis Fructus is a vine plant of Vitex rotundifolia Linne elephant. Or manhyeong (蔓荊) Vitex It is a ripe fruit of trifolia Linne (Verbenaceae). Fruit appearance is round or oval shape, outer side is black or black brown, and ⅓ to ⅔ from lower side is wrapped with thin calyx with grayish white color. When you look at the cross section of this medicine with a magnifying glass, the fruit is divided into four pieces and one white small seed is contained in it. There is a peculiar direction and the taste is sweet and slightly spicy. The pharmacological effects of sedation, pain relief, fever, and visceral circulation were reported.
Baekjaek (白 杏药) is a peony Paeonia It is the root of lactiflora Pallas or other related plants (Paeoniaceae and Paeoniaceae). It grows in deep mountains. It is 40 ~ 50cm in height. The roots are thick and fleshy, and the bottom is covered with scaly leaves. Leaves are 3 ~ 4 slices and split 3 ~ 2 times. The small leaf is long oval or the egg is turned upside down. The edge is flat and has no hair. The flowers bloom in June and are 4 ~ 5cm in diameter and run one at the end of the main stem. Calyx is ovate, 3, petals are inverted, 5 ~ 7. There are several operations and three or four ovaries. Fruits are red and undersized red seeds and mature black seeds. It has a hairy back on the back of the leaf (var.pilosa), a hair on the back of the leaf, a long style flower that grows backward and has a red flower (P.obovata) (povar.glabra). Roots are used for analgesia, gynecology, and women's diseases. It is distributed in Korea, Japan, China, Sakhalin islands.
Windshield (Saposhnikovia Root) is Saposhnikovia It is the root of divaricata Schischkin (mountain type and Umbelliferae). The roots are elongated, conical, tapering downward, 15 to 20 cm in length, and 7 to 15 mm in diameter. The outer surface is light brown, has many vertical wrinkles, and has fine roots. The body is light and the vagina is soft and easy to cut. The cut face has light brown skin, several openings, and the neck is light yellow. There is a peculiar smell and the taste is slightly sweet. It grows in dry sandy grass. It is about 1m in height. Branches are divided a lot. The seeds bloom in three years after budding. Many leaves come from roots. The leaves on the stem are alternate, petioles become shorter as they go upwards, and the lower part becomes sheaths and is divided into three horns. The split pieces are in the shape of a line, with sharp ends and a little stiffness. The flower blooms in July to August in white and runs on a stem and a branch with a double-bladed inflorescence. Small guns are small and 5-6. Ovary is subordinate. Fruit is split and flat, wide elliptical, and there are protrusions when young. Northeast of China, Huay Bay, and Inner Mongolia.
Ostericum Root is an Ostericum koreanum roots of Maximowicz or Chinese ganghwu Notopterygium incisum Ting or 寬 葉 羌 活Notopterygium forbesii Roots and roots of Boissier (mountain and Umbelliferae). It is prescribed to cold, headache, neuralgia, rheumatism, arthritis, paralysis. It is distributed in Korea (Gyeongbuk, Gangwon, Gyeonggi, Pyongbuk, Hamnam, Hambuk), and northeast of China. The roots of Ganghwam are conical or long conical, usually branched, 15 ~ 30 cm long, 2 ~ 5 cm in diameter. The outer surface is yellowish brown to brown, and there are beard roots or marks on it. There is a peculiar smell, the taste is sweet at first and cool afterwards and a little bit later. The roots and rootstocks of Chinese ganghwam are slightly curved, sometimes branched, 4 ~ 13 cm long and 6 ~ 25 mm in diameter. There is a fragrance and the taste is a little spicy. The roots or rootstocks of the houseplant Ganghwam are close to the cylindrical shape, and the stalks of the stem and leaf sheath remain at the upper end. Its roots are conical, with vertical wrinkles and peel eyes. There is a slight smell, and the taste is relatively cool.
Aralia cordata (獨活, Aralia Continentalis Root) is Aralia cordata Aralia It is the root of the continentalis Kitagawa (Araliaceae and Araliaceae). It grows in the mountains. The height is 1.5m and there is a little hair on the whole except flowers. Leaves are alternate phyllotaxis, length 50 ~ 100cm, butterfly 3 ~ 20cm, odd number of double haploid leaves, and light brown hairs when they are young. The small leaf is ovate or oval with sawteeth on the edge. The surface of the leaf is green, the back is white, and there is a small cotyledon on both sides of the petiole. Flower is bloomed from July to August with a large, light green bloom, conical flower growing, and blooming with a mountain-shaped inflorescence at the tip of a branch separated by a gun shape. Fruits are berries and ripen black in September-October. Roots are often used for muscle aches, paraplegic, headache, paralysis of paralysis. It is distributed in the mountains of East Asia.
Licorice is the Latin name Glycyrrhizae Radix et Rhizoma, Licorice, Glycyrrhiza uralensis Fischer, Glycyrrhia za glabra Linne or licorice and licorice Glycyrrhiza inflata Batalin (leguminosae) roots and / or rootstock as it is (ie, with the bark attached) or removed from the juniper. It has a peculiar smell and taste is sweet. Jinhae, geomung, muscle relaxation, diuretic action.
Ginseng (ginseng) is the root of Panax ginseng CA Meyer (Araliaceae and Araliaceae), or as it removes thin roots and cork layers. Pharmacological actions have been reported in cerebral cortex excitation and inhibition, equilibrium, anti-fatigue, anti-aging, immune enhancement, cardiac contraction, gonadal stimulation, hyperglycemia inhibition, protein synthesis promotion, homeostasis, anticancer and detoxification. Appearance is elongated columnar or fusiform type with 2 ~ 5 side roots and outer surface is light tan, with vertical wrinkles and thin root marks. Muscle head has an outline with a stem residue attached. In Korea, white ginseng (raw ginseng), red ginseng (red ginseng: steamed ginseng) and ginseng (蔘 蔘: thin roots) were recorded separately. In the private sector, however, they are distinguished from wild ginseng camphor and wild ginseng.
Shiho (Bupleurum Root) is the root of Bupleurum falcatum Linne or its variants (haploid and Umbelliferae). The roots are elongated cones or cylinders, single or split, 10-20 cm long, 5-15 mm in diameter. They are found in Korea, Japan, China, Mongolia, Siberia, Caucasus, Europe and live in grasses. There are no hairs in the whole aeration, and the branches are divided on the thin long stem. Roots are thick and short. The leaves are staggered, line-shaped or bar-shaped, the edges are flat, and the veins are parallel. The leaves on the roots are like petiole with the lower part narrowed and 10 ~ 30cm long. The leaves on the stem are 4 ~ 10cm long, 5 ~ 15mm long and the tip is pointed. Flowers bloom from August to September in yellow, with a double-inflorescence at the end of stem and branch. There are 2 ~ 7 peduncle, 5 ~ 10 flowers each. The gun flakes are narrow, narrow, split into 5 segments, 15 mm long, and the small guns are long elliptical or wider, 2.5-4 mm long. There are 5 petals and stamen and the ovary is lower (lower). Fruit is elliptical with division, about 3mm in length, ripened in September-October. Reproduction is either giving up or giving up seeds.
Cyperus Rhizome is a herb Cyperus Rotundus Linne (Chrysanthemum and Cyperaceae), the roots of the thin roots are removed. The brown and fur-like fibers in the nodules are not removed, and they are called curls. The removed ones are called incense rice, and they are called fragments. Rootstock mainly spindle type, length 1.5 ~ 3.5cm, diameter 0.5 ~ 1cm. Outer surface is brown to dark brown, has vertical wrinkle pattern, and stem is left on top. In addition, there are five to ten uneven ring nodes protruding, and there are traces of brown hairy roots and beard roots on the nodes of unfinished ones. The removal of the hairy roots in advance is relatively smooth, and the rings are not clear. Quality is hard. Roasted or boiled surface is yellowish brown to reddish brown and keratinized, and the dried surface is white and the powdery characteristic is clear. The endothelial layer is distinctive in its ring pattern, the color of the central region is relatively thick, and the vascular vasculature is scattered. There is a distinctive scent and taste is a little bit.
Ginger (ginger) is a fresh roots of Zingiber officinale Roscoe (ginger and Zingiberaceae). Southeast Asia is the origin and is grown as vegetables. The rootstock grows sideways and is fleshy, lumpy, yellowish, with a pungent flavor and a fragrant smell. At each node of the rootstock, a fake stem made of a sheath is straight up to a height of 30 to 50 cm, and the leaves are arranged in two rows at the top. Leaves are slanted, bar-shaped, with narrow ends on both ends and long bottom leaves. Pharmacological action to promote gastric secretion, digestive power, cardiac excitement, blood circulation promotion, and bacillary action have been reported.
Chengdu (川芎) is a Cnidium officinale Makino, China Taipei (中國 川芎) Ligusticum chuanxiong Hort. (Hill and Umbelliferae), a perennial herbaceous plant belonging to the mountain type. It is a Chinese plant and it is commonly cultivated in Korea. It has been reported that it has mildness, tastiness, soothing effect and hematopoiesis. In addition to raw materials, it is often taken in water.
Contrast (Jujube is the jujube tree Zizyphus There is JuJuba Miller. inermis Rehder or Bojang Jujube Trees Zizyphus There is JuJuba Miller. It is a ripe fruit of hoonensis TB Lee (Gramineae Rhamnaceae). Jujube Zizyphus There is JuJuba Miller. The inermis Rehder is Zizyphus Includes jujuba Miller. Fruits are elliptical or spherical, 2 to 3 cm long, and 1 to 2 cm in diameter. The outer surface is reddish brown to dark red with a fine wrinkle and shine. Both ends are slightly concave, with one end bearing a style and the other end bearing a fur seal. Surgical blood is thin and tough like a leather. The cecum is thick, dark gray brown, and is spongy, soft and sticky. Internal medicine The blood is hard fusiform, one end is pointed, the inside is divided into two spaces, the flat oval seed is contained and the quality is hard. There is a peculiar smell and the taste is sweet. The jujube jujube is almost elliptical compared to the fruit of the jujube, and the seed has almost no 仁.
The present inventors have found that all of the above extracts have a biological activity beneficial to the skin, and that the extracts of two or more of the above extracts, preferably Chimmae extract, Puerariae Radix extract, Panthera extract, , Ginseng Extract, Seoho Extract, Herbal Extract, Ginger Extract, Astragalus Extract, and Control Extract have a synergistic biological activity.
In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.
Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.
In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.
The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.
The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.
In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.
[Composition]
The composition according to the present invention is effective for extracting ginseng extract, Puerariae Radix extract, Panax ginseng extract, Vicinity extract, Windproof extract, Gangbyeol extract, Fragrant extract, Licorice extract, Ginseng extract, Seiho extract, Herbal extract, Ginger extract, As an ingredient.
In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.
Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of Ganoderma lucidum extract, Puerariae Radix Extract, Gentian Extract, Vicinity Extract, Windproof Extract, Ganghyeum Extract, Fragrant Extract, Licorice Extract, Ginseng Extract, Seiho Extract, Herbal Extract, Ginger Extract, : 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.
The composition according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010% 0.0021%, 0.002%, 0.002%, 0.002%, 0.0024%, 0.0025%, 0.002%, 0.002% , 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% %, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059% 0.0071%, 0.007%, 0.007%, 0.007%, 0.007%, 0.005%, 0.006%, 0.007% , 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092% %, 0.0095%, 0.0096%, 0.0097%, 0.0 0.025%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.005%, 0.08%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300% , 0.075%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950% %, 0.1000, 0.1250, 0.1500, 0.1750, 0.2000, 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250, 0.6500, 0.6750, 0.7000, 0.7250, 0.7500, 0.7750, 0.8000, 0.8250, 0.8500, 0.8750, , 0.9200%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2% , 2.4%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7% 4.5%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5% , 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1% 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 7%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9% , 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14% %, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% The composition may comprise one or more extracts of the present invention in the range of 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99%
The composition according to the present invention may also contain at least about 0.0001% to 90%, 0.001% to 90%, 0.01% to 90%, 0.1% to 90%, 0.1% to 90% %, 0.1 to 80%, 0.1 to 70%, 0.1 to 60%, 0.1 to 50%, 0.1 to 40%, 0.1 to 30%, 0.1 to 20% or 0.1 to 10% . ≪ / RTI >
The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.
The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.
The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.
The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.
The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.
In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .
The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.
May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.
The composition according to the present invention may be provided as a topical skin composition.
In addition, a method of topically applying the composition according to the present invention to the skin can be provided.
In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.
[Skin improvement effect]
The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.
More specifically, the composition according to the present invention has a skin whitening effect.
The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.
As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.
In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.
The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.
The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.
The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .
As a result of treating the complex extract according to the present invention, the present inventors confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And the effect of inhibiting the activity of skin elastase was shown to exert an effect corresponding to the known quercetin. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkles and elasticity improving effect.
Further, the composition according to the present invention has an effect of improving the trouble.
The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.
The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.
As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.
In addition, the composition according to the present invention has an antioxidative effect.
The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.
As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.
In addition, the composition according to the present invention has an antiherating effect.
The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.
As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.
Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.
Example 1 Preparation of Chimpanzee Extract
The gum was dried well and cut into small pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The chilled extract was filtered through a filter having a pore size of 0.2 mu m to prepare a chimaeric extract.
Example 2 Preparation of Puerariae Radix Extract
The dried persimmon was well dried and cut into small pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare Puerariae Radix extract.
≪ Example 3 > Production of mold extract
After the molds were well dried and finely cut, 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mold extract.
≪ Example 4 >
The vellus was dried well and cut three times. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a vile extract.
Example 5: Preparation of windshield extract
After drying thoroughly with a windshield, the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a windshield extract.
≪ Example 6 >
The mixture was thoroughly dried, and 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a gangbyeo extract.
Example 7 Preparation of Fragrant Extract
After thoroughly drying, 3 g of dry weight was put into a flask, and the mixture was extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a combed extract.
<Example 8> Preparation of ginseng extract
The ginseng was thoroughly dried, and then, 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a ginseng extract.
Example 9 Preparation of licorice extract
The licorice was dried well, and the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a licorice extract.
<Example 10> Preparation of Seiho Extract
After drying well and finely ground, 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a Seiho extract.
Example 11: Preparation of herbal extracts
The perfume was well dried and then placed in a flask. The flask was charged with 100 g of dry weight and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a herbal extract.
Example 12: Preparation of ginger extract
The ginger was well dried and cut into three pieces. 100 g of dry weight was put into a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare ginger extract.
≪ Example 13 >
The celestial ghouls were well dried, and then placed in a flask with a dry weight of 100 g and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a cucumber extract.
Example 14 Preparation of Control Extract
After the control was well dried, the flask was charged with 100 g of dry weight and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a control extract.
Example 15 Preparation of Mixed Extracts of Ginseng, Galangeng, Manjang, Vinegar, Windshield, Ganghwam, Fragrance, Licorice, Ginseng, Seiho, Ginger, Astragalus, and Control
In the same manner as in the above-mentioned examples, the same amount of the extracts were injected in the same amounts as the extracts of Chunma, Changgeun, Manjang, Baekjaek, Windbreak, Ganghwam, Ginseng, Seoho, Hyangbuja, Ginger, Astragalus, and control mixture were prepared.
≪ Experimental Example 1 >
In order to confirm anti-glycation efficacy, L-arginine and glucose were used to measure glycosylation-inhibiting activity.
First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine was added to each sample to be diluted to 50 ppm and used as a positive control. These samples were mixed well and finally glucose diluted with 1M phosphate buffer solution was added to the final concentration of glucose to 0.1 M and reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.
Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula.
[Equation 1]
('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group').
As shown in the results of Table 1 above, it can be seen that the mixed extract is superior to the aminoguanidine, which is known as an anticarious substance, in the anticarcinogenic effect.
<Experimental Example 2> Effect of inhibiting melanin formation
In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.
Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.
The amount of melanin was measured by an absorbance of 1 × 10 6 cells per unit cell, and the total amount of melanin relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 2 below. As shown in FIG.
As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect and was found to be useful for whitening purposes.
<Experimental Example 3> Anti-inflammatory effect
In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).
Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 5 wells into each well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.
&Quot; (2) "
NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100
As can be seen from the results of Table 3, it was found that the mixed extracts exhibited excellent activity as a natural substance when compared with the representative anti-inflammatory drug L-NMMA, although the relative activity was somewhat low.
<Experimental Example 4> Promoting collagen synthesis
The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.
For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.
As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was comparable to that of vitamin C, which is generally known to induce collagen synthesis.
<Experimental Example 5> Elastase activity inhibitory effect
The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.
Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastase was diluted with buffer solution to make a final concentration of 0.5 mM by making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.
&Quot; (3) "
The inhibition rate of elastase was calculated using Equation (3) and shown in Table 5 below.
As can be seen from the results in Table 5, when the mixed extract was treated, it was found that it exhibited a better inhibitory effect on elastase activity, though the effect was somewhat weaker than that of the positive control. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.
<Experimental Example 6> Antioxidative effect
The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.
First, 100 μl of mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).
IC 50 represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.
As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.
Claims (4)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101988295B1 (en) * | 2018-03-07 | 2019-06-12 | 한국 한의학 연구원 | Composition for skin moisturizing and anti-wrinkle comprising herbal medicine mixed extract as effective component |
KR20190143578A (en) * | 2018-06-21 | 2019-12-31 | (주)에스디생명공학 | Composition Comprising Enzyme Treated Extract of Red Ginseng and Gastrodia for Preventing or Improving Skin Wrinkle as Active Ingredient |
CN110859787A (en) * | 2019-12-11 | 2020-03-06 | 广州环亚化妆品科技有限公司 | Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof |
KR20220078062A (en) | 2020-12-03 | 2022-06-10 | 대한민국(환경부 국립생물자원관장) | Cosmetic composition containing Cyperus spp. extract for antiinflammation or skin whitening |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101988295B1 (en) * | 2018-03-07 | 2019-06-12 | 한국 한의학 연구원 | Composition for skin moisturizing and anti-wrinkle comprising herbal medicine mixed extract as effective component |
WO2019172626A1 (en) * | 2018-03-07 | 2019-09-12 | 한국 한의학 연구원 | Composition for skin moisturization and wrinkle reduction, containing extract of medicinal herb mixture as active ingredient |
KR20190143578A (en) * | 2018-06-21 | 2019-12-31 | (주)에스디생명공학 | Composition Comprising Enzyme Treated Extract of Red Ginseng and Gastrodia for Preventing or Improving Skin Wrinkle as Active Ingredient |
CN110859787A (en) * | 2019-12-11 | 2020-03-06 | 广州环亚化妆品科技有限公司 | Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof |
CN110859787B (en) * | 2019-12-11 | 2022-02-11 | 广州环亚化妆品科技有限公司 | Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof |
KR20220078062A (en) | 2020-12-03 | 2022-06-10 | 대한민국(환경부 국립생물자원관장) | Cosmetic composition containing Cyperus spp. extract for antiinflammation or skin whitening |
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