CN110859787B - Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof - Google Patents

Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof Download PDF

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CN110859787B
CN110859787B CN201911266683.4A CN201911266683A CN110859787B CN 110859787 B CN110859787 B CN 110859787B CN 201911266683 A CN201911266683 A CN 201911266683A CN 110859787 B CN110859787 B CN 110859787B
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chinese medicine
traditional chinese
medicine composition
glycation
preparation
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CN110859787A (en
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郑同妹
陈庆生
胡根华
吴知情
胡兴国
陈亮
龚盛昭
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Guangzhou Huanya Cosmetic Science and Technology Co Ltd
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Guangzhou Huanya Cosmetic Science and Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Abstract

The invention provides an anti-glycation traditional Chinese medicine composition and a preparation method and application thereof. The raw materials of the anti-saccharification traditional Chinese medicine composition comprise kudzu root, cinnamon, radix bupleuri, honeysuckle stem and salvia miltiorrhiza. The anti-glycation traditional Chinese medicine composition disclosed by the invention takes the kudzu root as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the salvia miltiorrhiza as a conductant drug, and the traditional Chinese medicine components are reasonable in compatibility and synergistic, so that the anti-glycation traditional Chinese medicine composition not only can remove free radicals and play a role in antioxidation, but also can inhibit the reaction process of sugar and protein, thereby achieving the purposes of improving the problems of skin aging, dark yellow and the like caused by skin glycation, and further achieving the skin care effects of fading fine wrinkles, dispelling yellow and brightening.

Description

Anti-saccharification traditional Chinese medicine composition and preparation method and application thereof
Technical Field
The invention belongs to the field of cosmetics, relates to a traditional Chinese medicine composition, a preparation method and application thereof, and particularly relates to an anti-saccharification traditional Chinese medicine composition, a preparation method and application thereof.
Background
Anti-glycation is one of the hottest topics since several years. "glycation" refers to the chemical reaction of excess sugar in the body with collagen to produce AGEs (advanced glycation end products). The reaction causes collagen to deteriorate, thereby causing skin to lose elasticity, dark yellow and loose. AGEs, the final products, are the leading cause of aging and aging-related health problems. Skin "glycation" is also one of the causes of skin aging.
Aging is a necessary and irresistible natural phenomenon in the life process of human beings. For intrinsic aging we cannot avoid, but for extrinsic aging we can accelerate skin aging by reducing, avoiding extrinsic causes. The fire-resistant sugar-resistant products in the market are expensive, the sugar-resistant effect is not ideal, and the active ingredients are difficult to play. Therefore, the development of the plant additive for cosmetics, which has the advantages of saccharification resistance, safety and no stimulation, becomes an industrial trend. Green, natural plant sources are the main direction of research. Based on the theory of traditional Chinese medicine, the purity of active ingredients is improved by applying modern extraction and separation technology according to the principle of monarch, minister, assistant and messenger formula, and the method becomes a new trend for developing plant efficacy raw materials.
CN103961276A discloses an anti-glycation cosmetic composition and an anti-glycation cosmetic containing the same. The anti-glycation cosmetic composition comprises 0.001-15 parts of an anti-glycation agent and 0.1-15 parts of an antioxidant; the anti-glycation agent comprises an anti-glycation agent A and an anti-glycation agent B; the antioxidant comprises a free radical network scavenger and an antioxidant A. The saccharifying agent A comprises puerarin, and the antioxidant A comprises bupleuri radix extract. However, they have a drawback that the anti-glycation effect is not satisfactory and the anti-glycation active ingredient hardly exerts its effect.
CN109568161A discloses a method for preparing a composition with anti-glycation and anti-skin aging functions, the composition with anti-glycation and anti-skin aging functions comprises an A phase and a B phase; wherein the phase A comprises: sclerotium rolfsii gum, 1.3 butanediol, glycerol and water; the phase B comprises: apple fruit cell culture extract, glycerol glucose, water, nicotinamide, glycerol, caprylyl glycol, xanthan gum, and Saviae Miltiorrhizae radix extract. Although the composition has a certain anti-saccharification effect, the skin care effects of fading fine lines and removing yellow and brightening are not obvious after long-term use.
Therefore, the development of a Chinese medicinal composition capable of inhibiting skin saccharification and improving skin aging and dark yellow is the focus of current research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an anti-saccharification traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition has reasonable compatibility and synergistic interaction of the traditional Chinese medicine components, can remove free radicals, has the effects of resisting oxidation and aging, and can inhibit the reaction process of sugar and protein, so that the problems of skin aging, dark yellow and the like caused by skin saccharification are solved, and the skin care effects of reducing fine lines, removing yellow and brightening are achieved.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides an anti-glycation traditional Chinese medicine composition, which is prepared from raw materials including kudzuvine root, cassia bark, Chinese thorowax root, honeysuckle stem and salvia miltiorrhiza.
The nature and the taste and the efficacy of the traditional Chinese medicines are as follows:
kudzu root: cool in nature, sweet and pungent in flavor. Has effects in promoting salivation, dredging meridian passage, promoting metabolism, and scavenging superoxide ion and hydroxyl free radical.
Cinnamon: pungent and sweet in flavor. Has effects of inducing fire to restore original qi, and warming and dredging channels and collaterals. Has antibacterial, antioxidant, tranquilizing, and antipyretic effects.
Bupleurum root: bitter, pungent and slightly cold in flavor. Has effects in dispelling and relieving fever, invigorating yang, relieving fever, relieving inflammation, and improving immunity.
Honeysuckle stem: sweet and cold in flavor. Has effects of clearing away heat and toxic materials, dispelling pathogenic wind, dredging collaterals, inhibiting bacteria, relieving inflammation, and improving immunity.
Red sage root: bitter and slightly cold in taste. Has effects in promoting blood circulation, dispelling blood stasis, relieving pain due to dysmenorrhea, cooling blood, and eliminating carbuncle, and also has antioxidant, antibacterial, and antiinflammatory effects.
The traditional Chinese medicine composition is used according to the principle of monarch, minister, assistant and guide. According to traditional Chinese medicine, the functions of viscera and essence, qi and spirit are considered to have important significance on aging, and the coordination of yin and yang is emphasized. In the formula, the kudzuvine root is taken as a monarch drug, and the kudzuvine root has the effects of invigorating spleen and stomach channels, expelling pathogenic factors from muscles and skin, reducing fever, promoting the production of body fluid, and clearing and activating the channels and collaterals; the ministerial drug is cinnamon, enters stomach, spleen, heart and liver channels, has the effects of supplementing fire and supporting yang, guiding fire to origin and warming and dredging channels and collaterals, can assist monarch drugs to reach targets and can coordinate yin and yang of the organism; the adjuvant drugs are radix bupleuri and caulis Lonicerae, have the effects of clearing heat and relieving fever, dispelling pathogenic wind and resolving stagnation, lifting yang qi, and stopping qi-flowing down , and can be used in combination with monarch and ministerial drugs to enhance therapeutic effect. The guiding drug is salvia miltiorrhiza, which has the effects of promoting blood circulation by removing blood stasis, stimulating the menstrual flow, cooling blood and eliminating carbuncle and plays a role in harmonizing the medicines in the formula.
The invention is based on the theory of traditional Chinese medicine, has reasonable compatibility of traditional Chinese medicines, and is suitable for the problems of skin elasticity loss, dark yellow, relaxation and the like caused by viscera deficiency, essence and qi failure and yin-yang imbalance. The traditional Chinese medicine composition takes the root of kudzu vine as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the radix salviae miltiorrhizae as a conductant drug, and the traditional Chinese medicine components are mutually matched and synergized, so that the reaction process of sugar and protein can be inhibited, and the problems of skin aging, dark yellow and the like caused by skin saccharification can be inhibited or improved, so that the skin is glossy and smooth, fine wrinkles are reduced, and the effects of dispelling the yellow and brightening the skin are achieved.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 1-40% of kudzu root, 1-40% of cinnamon, 1-40% of radix bupleuri, 1-40% of honeysuckle stem and 1-40% of salvia miltiorrhiza.
In the present invention, the content of pueraria lobata is 1-40% by weight, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%.
In the present invention, the weight percentage of cinnamon is 1-40%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%.
In the present invention, the weight percentage of the bupleurum root is 1-40%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%.
In the present invention, the weight percentage of the caulis lonicerae is 1-40%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%.
In the present invention, the weight percentage of the salvia miltiorrhiza is 1-40%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 10-40% of kudzuvine root, 10-30% of cinnamon, 10-30% of bupleurum, 5-20% of honeysuckle stem and 5-20% of salvia miltiorrhiza.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 20-40% of kudzuvine root, 15-30% of cinnamon, 15-30% of bupleurum, 5-20% of honeysuckle stem and 5-20% of salvia miltiorrhiza.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 30% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 10% of salvia miltiorrhiza.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 20% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 20% of salvia miltiorrhiza.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 30% of kudzuvine root, 30% of cassia bark, 30% of Chinese thorowax root, 5% of honeysuckle stem and 5% of salvia miltiorrhiza.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 40% of kudzuvine root, 15% of cinnamon, 15% of Chinese thorowax root, 15% of honeysuckle stem and 15% of salvia miltiorrhiza.
In a second aspect, the present invention provides a method for preparing the anti-glycation traditional Chinese medicine composition according to the first aspect, wherein the preparation method comprises the following steps:
(1) pretreating medicinal materials: crushing raw materials of each component of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder;
(2) extraction: adding a solvent into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution;
(3) concentration: and (3) filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition.
Preferably, the mesh number of the mixed powder in the step (1) is 100-200 meshes, for example, 100 meshes, 110 meshes, 120 meshes, 130 meshes, 140 meshes, 150 meshes, 160 meshes, 170 meshes, 180 meshes, 190 meshes, 200 meshes can be provided.
Preferably, the mass ratio of the mixed powder to the solvent in the step (2) is 1 (1-30), and may be, for example, 1:1, 1:2, 1:4, 1:6, 1:8, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20, 1:22, 1:24, 1:26, 1:28, 1: 30.
Preferably, the solvent of step (2) comprises water and/or ethanol.
Preferably, the extraction temperature in step (2) is 40-85 deg.C, such as 40 deg.C, 42 deg.C, 44 deg.C, 46 deg.C, 48 deg.C, 50 deg.C, 52 deg.C, 54 deg.C, 56 deg.C, 58 deg.C, 60 deg.C, 62 deg.C, 64 deg.C, 66 deg.C, 68 deg.C, 70 deg.C, 72 deg.C, 74 deg.C, 76 deg.C, 78 deg.C, 80 deg.C, 81 deg.C, 83 deg.C, 85 deg.C.
Preferably, the number of times of extraction in step (2) is 1-3, and may be, for example, 1, 2, or 3.
Preferably, the time of each extraction in step (2) is 1-3h, and can be 1h, 1.5h, 2h, 2.5h and 3 h.
Preferably, step (3) is followed by step (4) of purifying: and (4) eluting and concentrating the traditional Chinese medicine composition obtained in the step (3) for the second time to obtain a secondary concentrated solution, adding a solvent into the secondary concentrated solution for dissolving, and filtering.
Preferably, the elution step of step (4) is specifically: adsorbing the concentrated solution with macroporous resin, eluting with ethanol, and collecting eluate.
Preferably, the macroporous resin is any one of D101 or AB-8.
Preferably, the ethanol is present in a concentration of 30-95% by volume, for example 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%.
Preferably, the secondary concentration in the step (4) is reduced pressure concentration;
preferably, the solvent of step (4) is butanediol.
Preferably, the concentration of butanediol is 30-50% by volume, for example 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44%, 46%, 48%, 50%.
Preferably, the mass ratio of the solvent to the secondary concentrated solution in the step (4) is (0.5-10):1, and may be, for example, 0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10: 1.
Preferably, the preparation method specifically comprises the following steps:
(1) pretreating medicinal materials: crushing the raw materials of each component of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder of 100-200 meshes;
(2) extraction: adding a solvent into the mixed powder obtained in the step (1), and extracting for 1-3 times at 40-85 ℃ for 1-3h each time to obtain an extracting solution, wherein the mass ratio of the mixed powder to the solvent is 1 (1-30);
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: adsorbing the traditional Chinese medicine composition obtained in the step (3) by using macroporous resin, eluting by using 30-95% ethanol, collecting eluent, and performing secondary concentration to obtain secondary concentrated solution; finally adding a solvent for dissolving and filtering, wherein the mass ratio of the solvent to the secondary concentrated solution is (0.5-10) to 1.
In a third aspect, the present invention provides a use of the anti-glycation traditional Chinese medicine composition according to the first aspect in the preparation of cosmetics.
The traditional Chinese medicine composition is used as an additive in cosmetics, so that the aging problems of skin elasticity loss, dark yellow, relaxation and the like caused by skin saccharification can be effectively inhibited or improved.
In a fourth aspect, the present invention provides a cosmetic comprising the anti-glycation traditional Chinese medicine composition according to the first aspect.
Preferably, the anti-glycation traditional Chinese medicine composition is added in an amount of 0.05-20%, such as 0.05%, 0.1%, 0.2%, 0.5%, 1%, 2%, 5%, 8%, 10%, 12%, 15%, 18% or 20%, preferably 0.05-10% of the total weight of the cosmetic.
Preferably, the cosmetic comprises a mask, a lotion, a skin cream, a smoothing toner, a serum, a moisturizing cream, or a lotion.
Compared with the prior art, the invention has the following beneficial effects:
(1) the anti-saccharification traditional Chinese medicine composition disclosed by the invention takes the kudzu root as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the salvia miltiorrhiza as a conductant drug, and the traditional Chinese medicine components are reasonable in compatibility and synergistic, so that the anti-saccharification traditional Chinese medicine composition not only can remove free radicals and play a role in antioxidation, but also can inhibit the reaction process of sugar and protein, thereby achieving the purposes of improving the problems of skin aging, dark yellow and the like caused by skin saccharification, and further achieving the skin care effects of fading fine wrinkles, dispelling yellow and brightening.
(2) The anti-saccharification traditional Chinese medicine composition disclosed by the invention is pure natural in components, free of stimulation, safe and free of toxic and side effects, the clearance rate of DPPH free radicals can reach more than 62%, the clearance rate of hydroxyl free radicals can reach more than 69.5%, and the inhibition rate IE of non-enzymatic glycosylation can reach more than 33%.
Drawings
FIG. 1 is a bar graph showing the DPPH radical scavenging rate of the Chinese medicinal compositions prepared in examples 1 to 8 and comparative examples 1 to 13.
Fig. 2 is a bar graph showing the hydroxyl radical scavenging rate of the Chinese medicinal compositions prepared in examples 1 to 8 and comparative examples 1 to 13.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
The embodiment provides an anti-glycation traditional Chinese medicine composition, which is prepared from the following raw materials in percentage by weight: 30% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 10% of salvia miltiorrhiza.
The preparation method comprises the following steps:
(1) pretreating medicinal materials: pulverizing radix Puerariae 30g, cortex Cinnamomi 20g, bupleuri radix 20g, caulis Lonicerae 20g, and Saviae Miltiorrhizae radix 10g, sieving, and mixing to obtain 100 mesh mixed powder;
(2) extraction: adding 50% by volume of ethanol solution into the mixed powder obtained in the step (1), and extracting at 80 ℃ for 3 times, each time for 2 hours, so as to obtain an extracting solution, wherein the mass ratio of the mixed powder to the ethanol solution is 1: 15;
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: adsorbing the traditional Chinese medicine composition obtained in the step (3) by using AB-8 type macroporous resin, eluting for 3 times by using 85% ethanol, collecting eluent, and performing reduced pressure concentration until the mass ratio of the mass of the secondary concentrated solution to the total mass of the raw materials of each component of the traditional Chinese medicine composition in the step (1) is 1: 1; and finally adding butanediol to dissolve, wherein the mass ratio of the butanediol to the secondary concentrated solution is 1:1, and filtering.
Example 2
The embodiment provides an anti-glycation traditional Chinese medicine composition, which is prepared from the following raw materials in percentage by weight: 20% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 20% of salvia miltiorrhiza.
The preparation method comprises the following steps:
(1) pretreating medicinal materials: pulverizing radix Puerariae 20g, cortex Cinnamomi 20g, bupleuri radix 20g, caulis Lonicerae 20g, and Saviae Miltiorrhizae radix 20g, sieving, and mixing to obtain 150 mesh mixed powder;
(2) extraction: adding 70% by volume of ethanol solution into the mixed powder obtained in the step (1), and extracting at 75 ℃ for 3 times, wherein each time is 1h to obtain an extracting solution, and the mass ratio of the mixed powder to the ethanol solution is 1: 8;
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: adsorbing the traditional Chinese medicine composition obtained in the step (3) by using D101 type macroporous resin, eluting for 5 times by using 75% ethanol, collecting eluent, and performing reduced pressure concentration until the mass ratio of the mass of the secondary concentrated solution to the total mass of the raw materials of each component of the traditional Chinese medicine composition in the step (1) is 1: 1; and finally adding butanediol to dissolve, wherein the mass ratio of the butanediol to the secondary concentrated solution is 1:1, and filtering.
Example 3
The embodiment provides an anti-glycation traditional Chinese medicine composition, which is prepared from the following raw materials in percentage by weight: 30% of kudzuvine root, 30% of cassia bark, 30% of Chinese thorowax root, 5% of honeysuckle stem and 5% of salvia miltiorrhiza.
The preparation method comprises the following steps:
(1) pretreating medicinal materials: pulverizing radix Puerariae 30g, cortex Cinnamomi 30g, bupleuri radix 30g, caulis Lonicerae 5g, and Saviae Miltiorrhizae radix 5g, sieving, and mixing to obtain 150 mesh mixed powder;
(2) extraction: adding pure water into the mixed powder obtained in the step (1), and extracting for 3 times at 85 ℃ for 1.5h each time to obtain an extracting solution, wherein the mass ratio of the mixed powder to the pure water is 1: 20;
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: adsorbing the traditional Chinese medicine composition obtained in the step (3) by using AB-8 type macroporous resin, eluting for 4 times by using 65% ethanol, collecting eluent, and then carrying out reduced pressure concentration until the mass ratio of the mass of the secondary concentrated solution to the total mass of the raw materials of each component of the traditional Chinese medicine composition in the step (1) is 1: 1; and finally adding butanediol to dissolve, wherein the mass ratio of the butanediol to the secondary concentrated solution is 1:1, and filtering.
Example 4
The embodiment provides an anti-glycation traditional Chinese medicine composition, which is prepared from the following raw materials in percentage by weight: 40% of kudzuvine root, 15% of cinnamon, 15% of Chinese thorowax root, 15% of honeysuckle stem and 15% of salvia miltiorrhiza.
The preparation method comprises the following steps:
(1) pretreating medicinal materials: pulverizing 40g of radix Puerariae, 15g of cortex Cinnamomi, 15g of bupleuri radix, 15g of caulis Lonicerae, and 15g of Saviae Miltiorrhizae radix, sieving, and mixing to obtain 200 mesh mixed powder;
(2) extraction: adding 40% by volume of ethanol solution into the mixed powder obtained in the step (1), and extracting at 80 ℃ for 3 times, wherein each time lasts for 1.5h to obtain an extracting solution, and the mass ratio of the mixed powder to the ethanol solution is 1: 30;
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: adsorbing the traditional Chinese medicine composition obtained in the step (3) by using AB-8 type macroporous resin, eluting for 3 times by using 55% ethanol, collecting eluent, and performing reduced pressure concentration until the mass ratio of the mass of the secondary concentrated solution to the total mass of the raw materials of each component of the traditional Chinese medicine composition in the step (1) is 1: 1; and finally adding butanediol to dissolve, wherein the mass ratio of the butanediol to the secondary concentrated solution is 1:1, and filtering.
Example 5
The difference from example 1 is that: the traditional Chinese medicine composition comprises the following preparation raw materials in percentage by weight: 15% of kudzuvine root, 30% of cinnamon, 30% of Chinese thorowax root, 15% of honeysuckle stem and 10% of red-rooted salvia root, and the preparation method of the traditional Chinese medicine composition is the same as that of the embodiment 1.
Example 6
The difference from example 1 is that: the traditional Chinese medicine composition comprises the following preparation raw materials in percentage by weight: 30% of kudzuvine root, 10% of cinnamon, 30% of Chinese thorowax root, 15% of honeysuckle stem and 15% of red-rooted salvia root, and the preparation method of the traditional Chinese medicine composition is the same as that of the embodiment 1.
Example 7
The difference from example 1 is that: the traditional Chinese medicine composition comprises the following preparation raw materials in percentage by weight: 30% of kudzuvine root, 30% of cinnamon, 5% of Chinese thorowax root, 20% of honeysuckle stem and 15% of red-rooted salvia root, and the preparation method of the traditional Chinese medicine composition is the same as that of the embodiment 1.
Example 8
The difference from example 1 is that: the traditional Chinese medicine composition comprises the following preparation raw materials in percentage by weight: 20% of kudzuvine root, 20% of cinnamon, 15% of Chinese thorowax root, 15% of honeysuckle stem and 30% of red-rooted salvia root, and the preparation method of the traditional Chinese medicine composition is the same as that of the embodiment 1.
Comparative example 1
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 100g of kudzuvine root, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 2
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 100g of cinnamon, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 3
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from only 100g of radix bupleuri, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 4
The only difference from example 1 is that: the raw materials for preparing the traditional Chinese medicine composition are only 100g of honeysuckle stem, other traditional Chinese medicine components are not contained, and other preparation methods are the same.
Comparative example 5
The only difference from example 1 is that: the raw materials for preparing the traditional Chinese medicine composition are only 100g of salvia miltiorrhiza, other traditional Chinese medicine components are not contained, and other preparation methods are the same.
Comparative example 6
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 50g of kudzuvine root and 50g of cinnamon, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 7
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 40g of kudzuvine root, 30g of cinnamon and 30g of bupleurum, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 8
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 40g of kudzu root, 30g of cinnamon and 30g of honeysuckle stem, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 9
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 40g of kudzuvine root, 30g of cinnamon, 20g of honeysuckle stem and 10g of salvia miltiorrhiza, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 10
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 30g of cinnamon, 30g of radix bupleuri, 30g of honeysuckle stem and 10g of radix salviae miltiorrhizae, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 11
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 35g of kudzuvine root, 25g of Chinese thorowax root, 25g of honeysuckle stem and 15g of salvia miltiorrhiza, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 12
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 35g of kudzuvine root, 25g of cinnamon, 25g of Chinese thorowax root and 15g of salvia miltiorrhiza, does not contain other traditional Chinese medicine components, and has the same preparation method.
Comparative example 13
The only difference from example 1 is that: the traditional Chinese medicine composition is prepared from 32.5g of kudzuvine root, 22.5g of cinnamon, 22.5g of bupleurum and 22.5g of honeysuckle stem as raw materials, does not contain other traditional Chinese medicine components, and has the same preparation method.
Test example 1
Study of DPPH radical scavenging ability
The ability to scavenge DPPH free radicals reflects to some extent the antioxidant capacity of the material. The greater the free radical clearance, the greater the antioxidant capacity. Therefore, the antioxidant effect of the traditional Chinese medicine composition can be judged by researching the DPPH free radical scavenging capability of the traditional Chinese medicine composition.
2mL of DPPH test solution with a mass concentration of 47.7mg/L and a certain amount of the solution prepared in examples 1 to 8 were added to the test tubeThe total volume of the Chinese medicinal composition of (1) and the Chinese medicinal compositions prepared in comparative examples 1 to 13 is supplemented to 5mL by absolute ethyl alcohol, the mixture is shaken up and reacted in a dark place for a period of time, and then the absorbance at the wavelength of 517nm is measured by using a 1cm cuvette and is marked as As(ii) a Adding 2mL of DPPH test solution and 3mL of absolute ethyl alcohol into the test tube, and recording the measured absorbance as Ao(ii) a Adding 2mL of anhydrous ethanol and corresponding volume of the Chinese medicinal composition into the test tube, supplementing the total volume to 5mL with anhydrous ethanol, and recording the measured absorbance as Ar. The DPPH radical clearance (Y) is calculated as: y (%) ═ 1- (a)s－Ar)/A0X 100%, the specific test results are shown in table 1 and fig. 1:
TABLE 1
Figure GDA0003397655230000131
Figure GDA0003397655230000141
The test results show that the anti-glycation traditional Chinese medicine composition prepared in the embodiments 1-8 of the invention has good inhibition effect on DPPH free radicals, and the DPPH free radical clearance rate reaches more than 62%, wherein the embodiments 1-4 are the optimal technical scheme of the invention, and the DPPH free radical clearance rate reaches more than 93%. The invention fully shows that the traditional Chinese medicine composition takes the kudzu root as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the salvia miltiorrhiza as a conductant drug, the traditional Chinese medicine components are mutually matched and synergized, the traditional Chinese medicine composition has a good effect of eliminating DPPH free radicals, and has strong oxidation resistance.
Test example 2
Study of hydroxyl radical (. OH) inhibitory Capacity
Hydroxyl radical (. OH) is one of the most important oxygen radicals in the human body, the most active and toxic, and can cause damage to any molecular reactions in living cells. Therefore, the damage to human body can be reduced by inhibiting the hydroxyl radical (. OH) activity. The higher the inhibition rate, the stronger the antioxidant ability.
Taking 1.0mL of an absolute ethanol solution of 1.865mmol/L o-diazaphenanthrene in a test tube with a plug, respectively adding 2mL of a pH 7.4 phosphate buffer solution with the concentration of 0.2M and 1mL of samples with different concentrations, fully and uniformly mixing, adding 1.0mL of FeSO with the concentration of 1.865mmol/L4·7H2O solution, mixed again and then added with 1.0mL of 0.03% (V/V) H2O2Measuring absorbance value A of each group of mixed solution in constant temperature water bath at 37 ℃ for 60min at 536nmSUsing distilled water as blank to measure absorbance value AbReplacing H with distilled water2O2The absorbance A of the resulting specimen was measured as a lesion groupnThe hydroxyl radical scavenging rate of the antioxidant is calculated according to the following formula: hydroxyl radical scavenging rate (%) - (A)S-An)/(Ab-An)]X 100%. The specific test results are shown in table 2 and fig. 2:
TABLE 2
Figure GDA0003397655230000151
Figure GDA0003397655230000161
The test results show that the anti-glycation traditional Chinese medicine composition prepared in the embodiments 1-8 of the invention has good inhibition effect on hydroxyl radical, and the clearance rate of the hydroxyl radical reaches more than 69.5%, wherein the embodiments 1-4 are the optimal technical scheme of the invention, and the clearance rate of the hydroxyl radical reaches more than 99%. The invention fully shows that the traditional Chinese medicine composition takes the kudzu root as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the salvia miltiorrhiza as a conductant drug, the traditional Chinese medicine components are mutually matched and synergized, the traditional Chinese medicine composition has a good scavenging effect on hydroxyl free radicals, and has strong oxidation resistance.
Test example 3
Study of inhibition of non-enzymatic glycosylation
Enzymatic glycosylation is a complex series of non-enzymatic reactions, proteins and glucose inNon-enzymatic reactions occur in vivo, eventually leading to the formation of "aggressive" AGEs of skin glycation. Through research on non-enzymatic glycosylation reaction, the anti-glycosylation ability of the traditional Chinese medicine composition can be reflected. Under aseptic conditions, 20.00g/L bovine serum albumin solution sterilized with a 0.2 μm sterilizing membrane and 5.00mL each of 0.50mol/L glucose solution were added to a sterile cell culture flask, and 1% NaN was added310.00mL of 0.20mol/L phosphate buffer solution with pH 7.4, and establishing a complete glycosylation system control group a; meanwhile, a control group b without the traditional Chinese medicine composition and the glucose solution is set; adding a control group c containing no protein in the Chinese medicinal composition; and (4) a control group d added with the traditional Chinese medicine composition without adding glucose solution. Setting the dry pre-groups of the traditional Chinese medicine compositions in the examples 1-8 and the comparative examples 1-13 in the solution, and culturing for 28 days at constant temperature of 37 ℃. The tests were carried out on days 0, 2, 4, 8, 12, 16, 20, 24, 26, 28, respectively.
0.5mL of culture solution is taken out, diluted to 10mL, the fluorescence value F of the glycosylation end product at the excitation wavelength of 370nm and the emission wavelength of 440nm is measured, and the inhibition rate IE of non-enzymatic glycosylation is calculated. Formula for inhibition rate of non-enzymatic glycosylation IE: IE (%) ═ 1- (drug F-control)cF-controldF) V (control)aF-controlbF)]X 100%. Wherein the drug F is the fluorescence value of the sample to be tested and the contrastcF is the fluorescence value of the control group c, controldF is the fluorescence value of the control group d, controlaF is the fluorescence value of the control group a, controlbF is the fluorescence value of the control group b. After the glycosylated product is incubated, detecting the fluorescence intensity at 370nm/440nm, and calculating the inhibition rate, wherein the specific test results are shown in table 3:
TABLE 3
Figure GDA0003397655230000171
Figure GDA0003397655230000181
The test results show that the traditional Chinese medicine composition prepared in the embodiments 1 to 8 of the invention has strong ability to resist saccharification, can well inhibit non-enzymatic glycosylation, and has an inhibition rate IE of the non-enzymatic glycosylation of more than 33%, wherein the embodiments 1 to 4 are the optimal technical scheme of the invention, and the inhibition rate IE of the non-enzymatic glycosylation of more than 50%. The invention fully shows that the traditional Chinese medicine composition takes the kudzu root as a monarch drug, the cinnamon as a ministerial drug, the radix bupleuri and the honeysuckle stem as an adjuvant drug and the salvia miltiorrhiza as a conductant drug, the traditional Chinese medicine components are mutually matched and synergized, the traditional Chinese medicine composition has a good scavenging effect on hydroxyl free radicals, and has strong oxidation resistance.
Test example 4
Haemolysis test of erythrocytes
The erythrocyte hemolysis test is one of the alternatives of the rabbit eye irritation test (Draize test), and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the dissolution amount and denaturation degree of hemoglobin. The RBC test is used internationally for evaluating eye irritation studies on chemicals such as cosmetics and raw materials.
The Chinese medicinal compositions prepared in examples 1 to 8 and the Chinese medicinal compositions prepared in comparative examples 1 to 13 were subjected to an erythrocyte hemolysis test in accordance with the RBC test method and grade standard of European Alternational methods authentication center (ECVAM). ECVAM. ECVAM DB-ALM INVITOX Protoco, Red blood cells test system INVITOX No.37 Ispra. Italy ECVAM,1994 ]. The specific test method is shown in table 4:
TABLE 4
L/D Grading
﹥100 Has no irritation
10﹤L/D≤100 Micro-stimulation property
1﹤L/D≤10 Mild irritation
0.1﹤L/D≤1 Moderate irritation
L/D≤0.1 Severe irritation
The results of the hemolysis test on erythrocytes for the Chinese medicinal compositions prepared in examples 1 to 8 and the Chinese medicinal compositions prepared in comparative examples 1 to 13 are shown in Table 5, in which HD is50The concentration of the sample at which 50% of erythrocytes are hemolyzed, DI is the protein denaturation index, and L/D is HD50Ratio to DI.
TABLE 5
Figure GDA0003397655230000201
Figure GDA0003397655230000211
From the results of the erythrocyte hemolysis experiment in table 5, it can be seen that all of the traditional Chinese medicine compositions prepared in examples 1-8 of the present invention have an L/D of more than 100, and the irritation is classified as non-irritating, which indicates that all of the traditional Chinese medicine compositions of the present invention have the characteristics of mildness and no irritation, and that the sample concentration HD of the traditional Chinese medicine compositions prepared in examples 1-8 is at a concentration of 50% of erythrocytes when hemolysis50HD far greater than the extracts of the Chinese herbs prepared in comparative examples 1 to 1250Meanwhile, the protein denaturation index DI is also obviously smaller than the traditional Chinese medicine extracts prepared in comparative examples 1-12, which shows that the combination of the five traditional Chinese medicines is scientific and reasonable in compatibility, and the toxic and side effects and irritation of the traditional Chinese medicines can be obviously reduced after synergistic interaction, so that the aims of reducing the degree of cell hemolysis and protein denaturation are fulfilled.
Application example 1
The application example provides an essence formula containing the traditional Chinese medicine composition prepared in example 1, and the essence formula is prepared from the components with mass fractions shown in table 6 (namely, the numbers in the table represent the mass fractions, for example, 5 represents 5%):
TABLE 6
Figure GDA0003397655230000212
The preparation method of the essence comprises the following steps:
(1) weighing a small amount of deionized water, and uniformly spraying carbomer on the water surface until the carbomer is completely soaked for later use;
(2) slowly adding the completely dissolved acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer obtained in the step (1) and other substances into the residual water, and stirring until the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and other substances are completely dissolved;
(3) and (3) completely dissolving the mixture obtained in the step (2), and then performing vacuum degassing treatment.
Application example 2
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 2, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 3
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 3, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 4
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 4, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 5
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 5, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 6
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 6, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 7
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 7, and the content and the preparation method of other components are the same as those in the application example 1.
Application example 8
The application example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the example 8, and the content and the preparation method of other components are the same as those in the application example 1.
Application comparative example 1
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 1, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 2
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 2, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 3
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 3, and the content and the preparation method of other components are the same as those in the application example 1.
Application comparative example 4
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 4, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 5
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 5, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 6
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 6, and the content and the preparation method of other components are the same as those in the application example 1.
Application comparative example 7
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 7, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 8
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 8, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application 9
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 9, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 10
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 10, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 11
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 11, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 12
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 12, and the content and the preparation method of other components are the same as those in the application example 1.
Comparative application example 13
The application comparative example provides an essence formula, and the difference from the application example 1 is only that: the traditional Chinese medicine composition prepared in the example 1 in the component B is replaced by the traditional Chinese medicine composition prepared in the comparative example 13, and the content and the preparation method of other components are the same as those in the application example 1.
Test example 5
Human body trial evaluation experiment
The essence prepared in the application examples 1-8 and the essence prepared in the comparative examples 1-13 are respectively subjected to human trial evaluation, 220 volunteers with dark skin color are selected and randomly divided into 22 groups, and the essence prepared in the application examples 1-8 and the essence prepared in the comparative examples 1-13 are respectively used. The application method comprises smearing the composition once every morning and evening for 28 days, and filling out a trial evaluation table according to sensory evaluation. The composition is used for 7 days, 14 days and 28 days, and counted once. The volunteers evaluated the skin feel and anti-sugar effects of the samples during use, wherein the highest score was 5, indicating significant effect, and the lowest score was 1 indicating no effect. The specific test results are shown in table 7:
TABLE 7
Figure GDA0003397655230000251
Figure GDA0003397655230000261
From the evaluation results, the results of human trial evaluation show that the traditional Chinese medicine composition essence prepared in the application examples 1 to 8 has a good anti-sugar effect. The essence added with the traditional Chinese medicine composition has the anti-sugar effects of brightening the skin color, removing yellow and the like, and can restore the healthy state of elasticity and luster of rough and dull skin.
The applicant states that the present invention is described by the above examples to illustrate the anti-glycation traditional Chinese medicine composition of the present invention, and the preparation method and application thereof, but the present invention is not limited to the above examples, that is, the present invention is not limited to the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims (27)

1. The anti-saccharification traditional Chinese medicine composition is characterized by comprising the following preparation raw materials in percentage by weight: 1-40% of kudzu root, 1-40% of cinnamon, 1-40% of radix bupleuri, 1-40% of honeysuckle stem and 1-40% of salvia miltiorrhiza;
the anti-glycation traditional Chinese medicine composition is prepared by a preparation method comprising the following steps:
(1) pretreating medicinal materials: crushing raw materials of each component of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder;
(2) extraction: adding a solvent into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution, wherein the solvent comprises water and/or ethanol;
(3) concentration: and (3) filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition.
2. The anti-glycation traditional Chinese medicine composition according to claim 1, wherein the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 10-40% of kudzuvine root, 10-30% of cinnamon, 10-30% of bupleurum, 5-20% of honeysuckle stem and 5-20% of salvia miltiorrhiza.
3. The anti-glycation traditional Chinese medicine composition according to claim 2, wherein the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 20-40% of kudzuvine root, 15-30% of cinnamon, 15-30% of bupleurum, 5-20% of honeysuckle stem and 5-20% of salvia miltiorrhiza.
4. The anti-glycation traditional Chinese medicine composition according to claim 3, wherein the traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 30% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 10% of salvia miltiorrhiza.
5. The anti-glycation traditional Chinese medicine composition according to claim 3, wherein the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 20% of kudzuvine root, 20% of cinnamon, 20% of Chinese thorowax root, 20% of honeysuckle stem and 20% of salvia miltiorrhiza.
6. The anti-glycation traditional Chinese medicine composition according to claim 3, wherein the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 30% of kudzuvine root, 30% of cassia bark, 30% of Chinese thorowax root, 5% of honeysuckle stem and 5% of salvia miltiorrhiza.
7. The anti-glycation traditional Chinese medicine composition according to claim 3, wherein the preparation raw materials of the traditional Chinese medicine composition comprise, by weight: 40% of kudzuvine root, 15% of cinnamon, 15% of Chinese thorowax root, 15% of honeysuckle stem and 15% of salvia miltiorrhiza.
8. The method for preparing the anti-glycation traditional Chinese medicine composition according to any one of claims 1 to 7, wherein the preparation method comprises the following steps:
(1) pretreating medicinal materials: crushing raw materials of each component of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder;
(2) extraction: adding a solvent into the mixed powder obtained in the step (1) for extraction to obtain an extracting solution;
(3) concentration: and (3) filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition.
9. The method as claimed in claim 8, wherein the mixed powder of step (1) has a mesh size of 100-200 meshes.
10. The preparation method according to claim 8, wherein the mass ratio of the mixed powder to the solvent in the step (2) is 1 (1-30).
11. The method of claim 8, wherein the temperature of the extraction in step (2) is 40-85 ℃.
12. The method according to claim 8, wherein the number of times of the extraction in the step (2) is 1 to 3.
13. The method of claim 8, wherein the time for each extraction in step (2) is 1-3 h.
14. The method according to claim 8, further comprising a step (4) of purifying after the step (3): and (4) eluting and concentrating the traditional Chinese medicine composition obtained in the step (3) for the second time to obtain a secondary concentrated solution, adding a solvent into the secondary concentrated solution for dissolving, and filtering.
15. The preparation method according to claim 14, wherein the elution step of step (4) is specifically: adsorbing the concentrated solution with macroporous resin, eluting with ethanol, and collecting eluate.
16. The method for preparing the porous resin of claim 15, wherein the macroporous resin is D101 or AB-8.
17. The method according to claim 15, wherein the ethanol is present in a concentration of 30 to 95% by volume.
18. The method according to claim 14, wherein the secondary concentration in the step (4) is a concentration under reduced pressure.
19. The method according to claim 14, wherein the solvent in the step (4) is butanediol.
20. The method of claim 19, wherein the concentration of butanediol is 30-50% by volume.
21. The method according to claim 14, wherein the mass ratio of the solvent to the secondary concentrated solution in the step (4) is (0.5-10): 1.
22. The method according to claim 8, comprising the steps of:
(1) pretreating medicinal materials: crushing the raw materials of each component of the traditional Chinese medicine composition, sieving and uniformly mixing to obtain mixed powder of 100-200 meshes;
(2) extraction: adding a solvent into the mixed powder obtained in the step (1), and extracting for 1-3 times at 40-85 ℃ for 1-3h each time to obtain an extracting solution, wherein the mass ratio of the mixed powder to the solvent is 1 (1-30);
(3) concentration: filtering the extracting solution obtained in the step (2), and concentrating the filtrate to obtain the traditional Chinese medicine composition;
(4) and (3) purification: washing the traditional Chinese medicine composition obtained in the step (3) with macroporous resin, eluting with 30-95% ethanol, collecting eluent, and performing secondary concentration to obtain secondary concentrated solution; finally adding a solvent for dissolving and filtering, wherein the mass ratio of the solvent to the secondary concentrated solution is (0.5-10) to 1.
23. Use of the anti-glycation traditional Chinese medicine composition according to any one of claims 1-7 in the preparation of cosmetics.
24. A cosmetic comprising the anti-glycation traditional Chinese medicine composition according to any one of claims 1 to 7.
25. The cosmetic of claim 24, wherein the anti-glycation traditional Chinese medicine composition is added in an amount of 0.05-20% based on the total weight of the cosmetic.
26. The cosmetic of claim 25, wherein the anti-glycation traditional Chinese medicine composition is added in an amount of 0.05-10% based on the total weight of the cosmetic.
27. The cosmetic of claim 24, wherein the cosmetic comprises a mask, lotion, cream, essence, or lotion.
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