CN115531272B

CN115531272B - Antioxidant composition derived from alpine plants and preparation method and application thereof

Info

Publication number: CN115531272B
Application number: CN202211357150.9A
Authority: CN
Inventors: 张兴琪; 李雪珍; 苏潇雨; 刘瑞学; 冷群英
Original assignee: Guangdong Bawei Biotechnology Co ltd
Current assignee: Guangdong Bawei Biotechnology Co ltd
Priority date: 2022-04-18
Filing date: 2022-11-01
Publication date: 2023-09-26
Anticipated expiration: 2042-11-01
Also published as: CN115531272A

Abstract

The application discloses an antioxidant composition derived from mountain plants, which comprises the following components in parts by weight: 1-30 parts of saussurea involucrata extract, 1-30 parts of alpine leontopodium herb extract, 1-20 parts of sakura extract, 1-20 parts of gentian extract and 1-30 parts of selaginella extract; the composition has good antioxidation effect, can play a good role in scavenging free radicals when applied to cosmetics, can promote collagen generation, effectively relieves symptoms such as rough skin, obvious wrinkles, color spots and the like, has the advantages of pure nature, no stimulation, safety and no toxicity, can meet the requirements of safety and skin care, and has good popularization prospect and wide application market.

Description

Antioxidant composition derived from alpine plants and preparation method and application thereof

Technical Field

The application discloses a composition, in particular relates to an antioxidant composition derived from mountain plants, and a preparation method and application thereof, and belongs to the technical field of cosmetics.

Background

Aging is a necessary rule of human life process, is also a long-term proposition that skin care is old and unchanged, and can not be avoided for endogenous aging, but exogenous aging can be improved through conditioning and good life style. In recent years, with the enrichment of food culture and the increase of life pressure, many people begin to develop exogenous accelerated aging with different levels, and the problems of increased wrinkles, color spots and the like are particularly presented.

Conventional cosmetic formulations are not highly stable, and are prone to accumulation on the surface of the human body, although antioxidants such as vitamin C are added. CN108888565B discloses an antioxidant toner added with ginseng liposome and a preparation method thereof, wherein the formula comprises aureobasidium pullulans polysaccharide, betaine, sodium hyaluronate, ellagic acid, chenopodium faberi, total ginsenoside freeze-dried powder, ginseng liposome suspension, VC ethyl ether, ginseng essential oil and the like, and the formula has better moisturizing and antioxidant effects, but the formula components are more complex, and the single formulation is not beneficial to being applied to other types of cosmetics.

CN110934209a discloses a composition with antioxidant function, health food and preparation method thereof, the main preparation raw materials comprise: blueberry powder, green tea extract, saussurea involucrata extract culture, cherry powder, rosemary extract, etc. Although the patent granule has better antioxidation capability, the patent granule is required to be eaten and cannot be well applied to the field of cosmetics.

In northwest regions of China, most regions belong to plateau regions, and long-term severe conditions such as high cold, high radiation, hypoxia drought and the like bring about the characteristics of more plants and less plants in local snow, but still have the characteristics of strong growth of partial high-mountain antioxidant plants, and the long-term resistance to severe environments also enables the local plants to be rich in active ingredients such as more phenols, amino acids, flavone and the like. However, studies on cosmetic antioxidation of mountain plants have not been reported.

Therefore, it is necessary to develop a composition derived from mountain plants having good antioxidant efficacy.

Disclosure of Invention

In view of the above-mentioned shortcomings, the present application aims to provide an antioxidant composition derived from alpine plants, which has good antioxidant and antioxidant effects, can exert good free radical scavenging effects when applied to cosmetics, promote collagen production, and effectively relieve symptoms such as rough skin, obvious wrinkles, color spots, etc.

For this purpose, the first technical solution provided by the present application is as follows:

an antioxidant composition derived from mountain plants comprises the following components in parts by weight: 1-30 parts of saussurea involucrata extract, 1-30 parts of alpine leontopodium herb extract, 1-20 parts of sakura extract, 1-20 parts of gentian extract and 1-30 parts of selaginella extract.

The plant extracts in the application have good efficacy, and are specifically as follows:

the saussurea involucrata extract is an ideal natural antioxidant, contains saussurea involucrata extract lactone, amino acid and flavonoid, can effectively remove free radicals, and has the effects of tissue repair, anti-inflammatory, sun protection and the like.

The alpine leontopodium herb extract contains rich phenolic acids and minerals, has the effects of clearing heat, cooling blood, removing blood stasis, relieving pain, and resisting wrinkle, relieving, whitening and nourishing skin.

The sakura extract is rich in sakura extract flavone, sakura glycoside, sakura extract, vitamins and the like, has good effects of shrinking pores and balancing grease, and has excellent antioxidation capability.

The selaginella extract is activated when meeting water and has better antibacterial and moisturizing effects.

The gentian extract has the effects of relieving, calming and moistening skin, and has the effects of whitening and moisturizing on the premise of harmonizing the extracts of other plants.

In the plant extract composition of the present application. The herba Saussureae Involueratae extract and herba Saussureae Involueratae extract as the first effective components have effects of scavenging free radicals, relieving inflammation, and protecting against long-term injury caused by physiological or non-physiological factors by tissue repair and abundant minerals. The sakura extract is a second effective component, can assist the first effective component to exert the efficacy, contains active substances such as sakura extract glycoside, sakura extract essence, abundant vitamins and the like, and has good efficacy of shrinking pores and balancing grease. Herba Selaginellae extract as third effective component, has effects of promoting blood circulation, keeping moisture, promoting skin absorption, and can be combined with the first and second effective components to enhance therapeutic effect. The gentian extract is the fourth effective component, can relieve, calm and moisten skin, and can also play a role in harmonizing other plant extracts.

The second technical scheme provided by the application is as follows:

a method for preparing an antioxidant composition derived from alpine plants, comprising the steps of:

s1, pretreatment of raw materials: weighing herba Saussureae Involueratae, oriental cherry, herba Selaginellae, and radix Gentianae, pulverizing into powder with pulverizer, and mixing.

S2, extracting and separating: placing the powder obtained in the step S1 into an extraction tank, adding an extraction solvent for the first time, performing ultrasonic extraction for 1 time at 40-90 ℃ and 400W power, performing centrifugation for 5min at 4000r/min after 1-3h of extraction, and separating a first supernatant; adding extraction solvent for the second time, adding mixed enzyme, performing enzymolysis at 40-80deg.C for 1-3 hr, heating to 85deg.C, maintaining for 15 min, separating the second supernatant, and mixing the first supernatant and the second supernatant to obtain extractive solution.

S3, concentrating and refining: concentrating the extract obtained in the step S2 under reduced pressure to 1/3 to 1/4 of the original mass, adding the concentrated solution into macroporous adsorption resin for purification, eluting with ethanol solution with the volume ratio concentration of 10% -95%, collecting ethanol eluent, performing rotary evaporation concentration, dissolving butanediol with the total mass (the total mass of the concentrated solution and butanediol) accounting for 30%, and performing membrane filtration to obtain the final composition.

Preferably, the addition amount of the mixed enzyme in the S2 is 4000u/g, and the mixed enzyme is cellulase, pectase and protease according to the following formula 1:1:1, and mixing the materials in proportion.

Preferably, the extraction solvent in S2 is any one of water, ethanol, and an aqueous ethanol solution.

Preferably, the macroporous adsorption resin in the S3 is AB-8 type or D101 type resin.

Preferably, the membrane used for the membrane filtration in S3 is a microfiltration membrane.

The third technical scheme provided by the application is as follows: use of the antioxidant composition in cosmetics.

Preferably, the cosmetic is an external preparation for skin prepared by taking the antioxidant composition as an active component and adding conventional auxiliary materials or auxiliary ingredients of pharmacy or cosmetics.

Specifically, the cosmetic is one or more of toning lotion, essence, cream, facial mask and gel.

Compared with the prior art, the application has the following beneficial effects:

1. the antioxidant composition derived from the alpine plant disclosed by the application refers to the habit and the efficacy of various alpine plants, and the plant extracts are reasonably selected and proportioned, so that the antioxidant composition has good antioxidant efficacy, can promote collagen synthesis, effectively relieves symptoms such as rough skin, obvious wrinkles and color spots, has a certain whitening and moisturizing effect, and meets the requirement of effective skin care in a real sense.

2. The antioxidant composition derived from the mountain plant disclosed by the application has the advantages of pure nature, no stimulation, safety and no toxicity, can meet the requirements of safety and skin care, and has good popularization prospect and wide application market.

Drawings

FIG. 1 is a graph showing the results of the average measurement of the score of wrinkles in experiment 5;

FIG. 2 is a graph showing the measurement result of the skin firmness F4 in experiment 5.

Detailed Description

The following claims are presented in further detail in connection with the detailed description, but are not to be construed as limiting the application, as any person who makes a limited number of modifications within the scope of the claims is within the scope of the claims.

Example 1

An antioxidant composition derived from alpine plants, comprising the following components: 20g of saussurea involucrata extract, 20g of alpine leontopodium herb extract, 10g of sakura extract, 10g of selaginella extract and 10g of gentian extract.

Example 2

An antioxidant composition derived from alpine plants, comprising the following components: 17.5g of saussurea involucrata extract, 17.5g of alpine leontopodium herb extract, 17.5g of sakura extract, 8.75g of selaginella extract and 8.75g of gentian extract.

Example 3

An antioxidant composition derived from alpine plants, comprising the following components: 14g of saussurea involucrata extract, 14g of alpine leontopodium herb extract, 14g of sakura extract, 14g of selaginella extract and 14g of gentian extract.

Example 4

An antioxidant composition derived from alpine plants, comprising the following components: 30g of saussurea involucrata extract, 10g of alpine leontopodium herb extract, 10g of sakura extract, 10g of selaginella extract and 10g of gentian extract.

Example 5

An antioxidant composition derived from alpine plants, comprising the following components: 5g of saussurea involucrata extract, 30g of alpine leontopodium herb extract, 10g of sakura extract, 15g of selaginella extract and 10g of gentian extract.

Example 6

An antioxidant composition derived from alpine plants, comprising the following components: 5g of saussurea involucrata extract, 15g of alpine leontopodium herb extract, 30g of sakura extract, 5g of selaginella extract and 15g of gentian extract.

Example 7

An antioxidant composition derived from alpine plants, comprising the following components: 15g of saussurea involucrata extract, 10g of alpine leontopodium herb extract, 10g of sakura extract, 30g of selaginella extract and 5g of gentian extract.

Example 8

An antioxidant composition derived from alpine plants, comprising the following components: 5g of saussurea involucrata extract, 10g of alpine leontopodium herb extract, 5g of sakura extract, 20g of selaginella extract and 30g of gentian extract.

Examples preparation examples:

S2, extracting and separating: placing the powder obtained in the step S1 into an extraction tank, adding an extraction solvent for the first time, wherein the addition amount is 15 times of the total mass of the raw materials, performing ultrasonic extraction for 1 time at 65 ℃ with 400w of power for 1.2 hours, and separating a supernatant fluid of the first time; adding an extraction solvent for the second time, wherein the addition amount is 15 times of the total mass of the raw materials, and adding cellulase: pectase: protease is 1:1:1, the adding amount of the mixed enzyme is 4000u/g (based on the total mass of the raw materials), cooling to 55 ℃ for enzymolysis for 2 hours, then heating to 85 ℃, preserving heat for 15 minutes, separating the supernatant of the second time, and combining the supernatant of the first time and the supernatant of the second time to obtain an extracting solution.

S3, concentrating and refining: concentrating the extract obtained in the step S2 under reduced pressure to 1/3 to 1/4 of the original mass, adding the concentrated solution into macroporous adsorption resin for purification, eluting with 70% ethanol solution by volume, collecting ethanol eluent, performing rotary evaporation and concentration, adding butanediol with the total mass (the total mass of the concentrated solution and butanediol) accounting for 30%, dissolving, and performing membrane filtration to obtain the final composition.

In order to demonstrate the effect of the present application, comparative experimental examples of the present application are given below:

the preparation method of any one of the compositions of comparative examples 1 to 5 was the same as in examples.

Comparative example 1

An antioxidant composition derived from alpine plants, comprising the following components: 20g of alpine leontopodium herb extract, 20g of sakura extract, 20g of selaginella extract and 10g of gentian extract.

Comparative example 2

An antioxidant composition derived from alpine plants, comprising the following components: 20g of saussurea involucrata extract, 20g of sakura extract, 20g of selaginella extract and 10g of gentian extract.

Comparative example 3

An antioxidant composition derived from alpine plants, comprising the following components: 20g of saussurea involucrata extract, 10g of alpine leontopodium herb extract, 20g of selaginella extract and 20g of gentian extract.

Comparative example 4

An antioxidant composition derived from alpine plants, comprising the following components: 20g of saussurea involucrata extract, 20g of alpine leontopodium herb extract, 10g of sakura extract and 20g of gentian extract.

Comparative example 5

An antioxidant composition derived from alpine plants, comprising the following components: 20g of saussurea involucrata extract, 20g of alpine leontopodium herb extract, 10g of sakura extract and 20g of selaginella extract.

The antioxidant composition can be used for preparing antioxidant cosmetics. The antioxidant cosmetic is an external preparation for skin, which is prepared by taking an antioxidant composition as an active component and adding conventional auxiliary materials or auxiliary components of pharmacy or cosmetics. When the composition is specifically used, the composition prepared by the application is directly added into a cosmetic matrix, and the addition amount can be adjusted according to actual requirements, preferably 0.5-10wt%.

Sample example

Preparation of antioxidant moisturizing cream: the antioxidant compositions prepared in the examples were added to the formulations to prepare creams, and the antioxidant cream formulations containing the antioxidant compositions are given in Table 1, with the amounts of each substance expressed in mass fractions.

TABLE 1 antioxidant facial cream formulation

The preparation method of the antioxidant face cream comprises the following steps: mixing and stirring the components of the phase A, heating to 80 ℃, and fully and uniformly dispersing for later use; mixing, stirring and heating the phase B to 85 ℃, and fully and uniformly dispersing; adding the treated phase A into the phase B, fully homogenizing, dispersing uniformly, and then stirring and cooling; cooling to below 70 ℃, adding the phase C, fully homogenizing, stirring uniformly, cooling to below 60 ℃, adding the phase D, and stirring uniformly; and (3) after the temperature is reduced to below 50 ℃, adding the E phase, uniformly stirring, and discharging after the detection is qualified.

To better illustrate the advantages of the present application, the efficacy test of the composition with antioxidant efficacy provided by the present application is given below:

experiment 1 antioxidant efficacy test-DPPH free radical scavenging experiment

Accurately weighing 41.67mg of DPPH reagent, fixing the volume to a 100mL brown volumetric flask, and taking 5mL of the reagent to a 50mL brown volumetric flask by using absolute ethyl alcohol to obtain DPPH test liquid (0.1 mmol/L). 1mL of the PPH test solution was removed and 0.5mL of the extracts prepared in examples 1 to 8 and comparative examples 1 to 5 were added thereto, respectively, at different concentrations as shown in Table 2. After fully mixing, standing for 30min in the dark, taking absolute ethyl alcohol as a blank control, measuring the absorbance at 517nm, setting vitamin C as a control, calculating the clearance rate of each extracting solution to DPPH according to the following formula, and calculating the semi-inhibition concentration IC50 value: DPPH clearance (%) = [1- (As-Ar)/A0 ]. Times.100%

Wherein: as-absorbance of the extract and DPPH reaction system; ar-absorbance of extract-absolute ethanol; a0—absorbance of the control absolute ethanol.

TABLE 2 Table of compositions of reaction liquids

Absorbance of light	Extract addition (mL)	DPPH testing solution (mL)	Absolute ethyl alcohol (mL)
				A0	0	1	0.5
Ar	0.5	0	1
				As	0.5	1	0

The DPPH free radical scavenging rate reflects the antioxidant capacity to a certain extent, and the higher the scavenging rate is, the better the antioxidant degree is, and the better the antioxidant effect is, so that the antioxidant effect can be judged by researching the DPPH free radical scavenging capacity of the composition. Vitamin C is a high-efficiency antioxidant which is recognized by the cosmetic industry, and the experiment uses vitamin C as a control to examine the antioxidant capacity of the vitamin C.

As can be seen from Table 3, the half inhibitory concentration (dry weight basis) of DPPH of vitamin C as a control was 38.39. Mu.g/mL, whereas the half inhibitory concentration of experimental example 2 was 58.46. Mu.g/mL, which is slightly higher than the IC50 value of vitamin C, within the same scale, indicating that the composition had good DPPH scavenging ability. Meanwhile, as can be seen from the following table 3, the IC50 values of examples 1 to 8 are smaller than those of comparative examples 1 to 5, which shows that the antioxidant capacity of the composition comprising five plant extracts is superior to that of the composition lacking any one plant extract, the formulation compatibility is reasonable, and the synergistic effect can be achieved, and the good antioxidant effect can be jointly exerted.

TABLE 3 Table of the results of DPPH radical scavenging experiments on antioxidant compositions

Sample name	DPPH half clearance IC50 (μg/mL)
		Vitamin C	38.39
Example 1	67.25
		Example 2	58.46
Example 3	69.54
		Example 4	78.84
Example 5	74.26
		Example 6	69.72
Example 7	88.95
		Example 8	84.63
Comparative example 1	107.32
		Comparative example 2	135.65
Comparative example 3	142.63
		Comparative example 4	98.56
Comparative example 5	97.29

Experiment 2 antioxidant efficacy test-hydroxyl radical scavenging experiment

Experiments were performed with the plant compositions prepared in experimental examples 1 to 8 and comparative examples 1 to 5. The test is as follows:

the Fenton reaction is adopted to generate hydroxyl free radicals. 1.0. 1.0mLH is added into the reaction system ₂ O ₂ Solution (9.0 mmol/L), 1mL salicylic acid-ethanol solution (9.0 mmol/L), 1mL antioxidant composition with the same concentration, and 1mLH ₂ O ₂ (8.8 mmol/L), in a water bath at 37 ℃ for 30min, absolute ethyl alcohol is used as a blank control, the absorbance is measured at 510nm, and vitamin C is used as a control, and the clearance rate of each extracting solution to hydroxyl is calculated according to the following formula:

hydroxyl group clearance (%) = [1- (Ax-Ax 0)/A0 ] ×100%

Wherein: ax-absorbance of the extract reaction system; ax 0-does not add H ₂ O ₂ Absorbance of the sample (substitution of absolute ethanol for H) ₂ O ₂ ) The method comprises the steps of carrying out a first treatment on the surface of the A0—absorbance of the control absolute ethanol.

TABLE 4 results of experiments on the scavenging ability of compositions to hydroxyl radicals

Sample name	Clearance of hydroxy (%)
		Vitamin C	85.3
Example 1	83.5
		Example 2	82.6
Example 3	79.6
		Example 4	78.3
Example 5	77.3
		Example 6	79.5
Example 7	66.5
		Example 8	65.3
Comparative example 1	60.2
		Comparative example 2	58.6
Comparative example 3	53.9
		Comparative example 4	68.5
Comparative example 5	70.3

Vitamin C was used as a positive control to examine its hydroxyl radical scavenging ability. As shown in Table 4 above, the examples had a clearance rate similar to that of vitamin C, especially example 1 up to 83.5% clearance rate, when added to compositions of comparable mass concentration, and as shown by vitamin C and its proximity, indicating that the formulated compositions had good scavenging ability of hydroxyl radicals. And the clearance rate of the examples 1-8 is higher than that of the comparative examples 1-5, which shows that the antioxidant capacity of the composition containing five plant extracts is better than that of the composition lacking any plant extract, the five plant extracts have synergistic effect, the formula compatibility is reasonable, and the composition further shows that the composition has good antioxidant capacity.

Experiment 3, cell level-anti-wrinkle efficacy test

The composition prepared in example 1 was chosen for testing as follows:

cytotoxicity detection HFF-1 cell suspension was added to a 96-well plate and cultured at 37℃under 5% CO2 for 24 hours. A series of concentration gradient samples were prepared and incubated in 96-well plates at 37℃and 5% CO2 for 24h. The culture medium was discarded, washed 2 times with PBS, and incubated with 50 ul/well of MTT solution at 37℃under 5% CO2 for 2-6h. The MTT-containing medium was discarded, DMSO was added at 150 ul/well, and after shaking for 5-10min, OD was measured at 570 nm. The concentration without significant cytotoxicity was selected for the protein assay experiments.

Type i collagen content determination: HFF-1 cell suspension was added to a 96-well plate and cultured at 37℃under 5% CO2 for 24 hours. Discarding the old culture medium, molding by UVB induction, adding 100ul of DMEM culture medium containing samples into each hole after irradiation, simultaneously setting a blank culture medium control group and a culture medium containing TGF-beta as positive control groups, and culturing for 24 hours + -2 hours under the condition of 37 ℃ and 5% CO 2. After the cultivation, the content of human type I collagen is determined according to the ELISA kit instructions. And (3) taking the concentration of the standard substance as an abscissa and the OD450 value as an ordinate, preparing a standard curve, and taking the standard curve according to the OD450 value of the sample to obtain the collagen content of the experimental group. The average of 3 wells per group was finally selected as the final collagen result. Statistical and significance analysis was performed using GraphPadPrism or other statistical software, P values were calculated, P < 0.05 indicating significant differences, otherwise no significant differences.

Up-regulation (%) = (T/C-1) ×100%

Wherein: t-average collagen value of experimental group

C-average value of blank/solvent group collagen

Table 5, results of experiments on collagen content of the compositions

The results of the composition on collagen content are shown in the table 5, nc is a blank control, and PC is a positive control. As shown in the table, when the sample of the example 1 in the safe concentration range is added, the average value of the collagen in the cells is 0.34ng/ml, compared with the NC group, the collagen content is up-regulated by 88.34 percent, and compared with the positive group, the collagen content is slightly lower, so that the composition has good effect of promoting the collagen synthesis of the cells and can play a good anti-wrinkle effect.

Experiment 4, human body Patch test

The present project evaluates the irritation of the antioxidant composition by the human skin patch test. A cream base containing 5.0% and 10.0% of the plant antioxidant composition of examples 1-3 was prepared for closed patch, and the possibility of adverse reaction of the sample on human body was examined. In the test, sample 1 was a cream to which 5.0% of the plant antioxidant composition was added, and sample 2 was a cream to which 10.0% of the plant antioxidant composition was added. The skin adverse reactions were ranked as shown in table 6 below:

TABLE 6 skin adverse reactions grading Table

Subject patch tests were performed on the creams prepared in examples 1-3 above, with a patch time of 24 hours for 30 persons (aged between 20 and 50 years), and skin reactions of subjects were observed when subjects removed the patch tester for 30min, 24 hours and 48 hours, and the results are shown in table 7 below.

TABLE 7 skin reaction conditions of subjects

As can be seen from the table, the cream added with the plant antioxidant composition has no adverse irritation reaction to human body and good safety.

Experiment 5 in vitro evaluation experiment of human body

1. Experimental method

The antioxidant moisturizing facial cream prepared in application example 1 was used for 20 volunteers selected at random. Test population: 20 healthy volunteers with wrinkles such as dry lines, fine lines, fish tails, expression lines and the like are selected, and the external preparation is not applied, and has no allergy to cosmetics, no acute inflammation on the face, no hormone medicines or immunosuppressants, no participation in other clinical tests and no application.

The test method comprises the following steps: after each group of volunteers cleaned the skin with warm water, the product was uniformly applied to the face 1 time each day in the morning and evening, and after 2 and 4 weeks of continuous use, the skin was tested using a visual facial image analyzer, skin firmness test probe, and the like, respectively. Each time a facial image was taken and analyzed for wrinkle parameters, skin firmness, and 10 cycles per site were tested and averaged. The results are shown in FIGS. 1-2 (note: D0, D14, D28 represent the results of the test on days 0, 14, 28, respectively).

As shown in fig. 1-2, after 20 volunteers use the cream base added with 5% of the antioxidant composition, the average value of the wrinkle score is reduced by 18.87% and 24.48% in days D14 and D28 respectively, which indicates that the composition can effectively improve the conditions of obvious and deepened wrinkles, and achieve the anti-wrinkle effect; the average value of the skin tightness F4 is reduced by 6.84 percent and 23.62 percent in D14 and D28 respectively, which shows that the skin tightness F4 can effectively improve the skin relaxation and promote the expression of related elastin in the skin and has good tightening effect. ( And (3) injection: the higher the wrinkle score, the more visible the skin wrinkles; the smaller the skin tightness F4, the tighter the skin is )

In conclusion, the application carries out scientific compatibility on saussurea involucrata, alpine leonia, sakura, gentian and the like, and prepares the antioxidant composition through extraction and purification technologies such as ultrasonic, biological enzymolysis, column chromatography and the like, and the composition has good effects of scavenging free radicals, promoting collagen generation, tightening, resisting wrinkles and the like, and can effectively relieve rough skin, obvious wrinkles, color spots and the like, and deeply improve the skin state.

The above examples are preferred embodiments of the present application, but the embodiments of the present application are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present application should be made in the equivalent manner, and the embodiments are included in the protection scope of the present application.

Claims

1. An antioxidant composition derived from mountain plants is characterized by comprising the following components in parts by weight: 1-30 parts of saussurea involucrata extract, 1-30 parts of alpine leontopodium herb extract, 1-20 parts of sakura extract, 1-20 parts of gentian extract and 1-30 parts of selaginella extract,

the preparation method of the antioxidant composition comprises the following steps:

s1, pretreatment of raw materials: weighing herba Saussureae Involueratae, oriental cherry, herba Selaginellae, and radix Gentianae, pulverizing into powder with pulverizer, and mixing;

s2, extracting and separating: placing the powder obtained in the step S1 into an extraction tank, adding an extraction solvent for the first time, performing ultrasonic extraction for 1 time at 40-90 ℃ and 400w of power, centrifuging at 4000r/min for 5 minutes after 1-3 hours of extraction, and separating a first supernatant; adding extraction solvent for the second time, adding mixed enzyme, performing enzymolysis at 40-80deg.C for 1-3 hr, heating to 85deg.C, maintaining for 15 min, separating the second supernatant, and mixing the first supernatant and the second supernatant to obtain extractive solution;

s3, concentrating and refining: concentrating the extract obtained in the step S2 under reduced pressure to 1/3 to 1/4 of the original mass, adding the concentrate into macroporous adsorption resin for purification, eluting with ethanol solution with the volume ratio concentration of 10% -95%, collecting ethanol eluent, performing rotary evaporation concentration, adding the concentrate into butanediol with the total mass ratio of butanediol of 30%, dissolving, and performing membrane filtration to obtain the final composition.

2. The antioxidant composition derived from mountain plants as set forth in claim 1, wherein the amount of the mixed enzyme in S2 is 4000u/g, and the mixed enzyme is cellulase, pectase and protease according to 1:1:1, and mixing the materials in proportion.

3. The antioxidant composition of claim 1, wherein the S2 extraction solvent is any one of water, ethanol or aqueous ethanol solution.

4. An antioxidant composition derived from mountain plants as set forth in claim 1, wherein said macroporous adsorption resin in S3 is AB-8 type or D101 type resin.

5. Use of the antioxidant composition according to any one of claims 1 to 4, in cosmetics.

6. The use according to claim 5, wherein the cosmetic is an external preparation for skin prepared by adding conventional auxiliary materials or auxiliary ingredients for pharmaceutical or cosmetic to the antioxidant composition as an active ingredient.

7. The use according to claim 5, wherein the cosmetic is one or more of a lotion, a serum, a cream, a mask, a jelly.