CN111714434B

CN111714434B - Ceramide compound muscle-soothing and skin-activating factor and cream

Info

Publication number: CN111714434B
Application number: CN202010457809.2A
Authority: CN
Inventors: 刘育财; 刘泽洪
Original assignee: Foshan Gubang Biotechnology Co ltd
Current assignee: Foshan Gubang Biotechnology Co ltd
Priority date: 2020-05-26
Filing date: 2020-05-26
Publication date: 2021-04-02
Anticipated expiration: 2040-05-26
Also published as: CN111714434A

Abstract

The invention discloses a ceramide compound muscle-soothing and skin-activating factor and cream; aims to provide a skin activating factor which has no stimulation to skin, improves dark skin color, delays skin aging, replenishes water and moisturizes, effectively repairs damaged skin barriers, and a cream which can replenish water and moisturize, delay skin aging and repair skin barriers; the skin-activating factor for calming the skin comprises 120-27 parts of ceramide, 10-13 parts of plant compound oil, 8-11 parts of lupin seed extract, 8-11 parts of platycladi seed extract, 6-9 parts of cocklebur fruit extract, 3-6 parts of crocus sativus flower extract, 3-6 parts of scutellaria baicalensis root extract, 2-4 parts of actinidia arguta extract and 2-4 parts of snail secretion filtrate; belongs to the field of cosmetics.

Description

Ceramide compound muscle-soothing and skin-activating factor and cream

Technical Field

The invention relates to a skin-activating factor for soothing the muscles, and also relates to cream containing the skin-activating factor for soothing the muscles, belonging to the technical field of cosmetics.

Background

The physical barrier of skin is composed of sebum membrane, horny layer keratin, lipid, sandwich structure, brick wall structure, mucopolysaccharide, etc., and has effects in resisting external harmful substances, irritants, and ultraviolet, keeping moisture, regulating inflammation, and resisting aging.

However, due to the increasing serious environmental pollution, ultraviolet irradiation, closed air-conditioning environment, poor living habits, especially poor skin care habits, such as skin barrier damage caused by long-term use of cosmetics containing hormones, lead and mercury and a large amount of essence and preservatives, dry skin, dull skin, rough skin with large pores, accelerated skin aging, thinned cuticle, red and dull skin, atopic dermatitis, eczema, psoriasis, ichthyosis, solar dermatitis and other skin sensitivity, irritant dermatitis, hormone-dependent dermatitis and other skin greasiness, seborrheic diseases such as acne, rosacea and seborrheic dermatitis, and accelerated skin aging problems.

In addition, as the aging of the skin progresses, the decline of APSC multipotential cells in vivo is caused, dead skin adheres to the skin surface without falling off, and the skin aging is accelerated. In addition, excessive exposure of the skin to sunlight results in excessive oxidation of the skin, leading to skin aging.

However, skin care products on the market at present generally have single effect, and cannot meet the multiple effects of soothing the skin, moisturizing, delaying skin aging and the like, and repair skin barriers.

Disclosure of Invention

In view of the above problems, a first object of the present invention is to provide a skin activating factor which is non-irritating to the skin, improves dull skin color, delays skin aging, moisturizes water and effectively repairs the damaged skin barrier.

The second purpose of the invention is to provide a cream for moisturizing, delaying skin aging and repairing skin barriers.

In order to solve the above technical problems, a first technical solution provided by the present invention is as follows:

a ceramide compound muscle-soothing and skin-activating factor comprises the following components in parts by weight: 120-27 parts of ceramide, 10-13 parts of plant compound oil, 8-11 parts of lupin seed extract, 8-11 parts of platycladi seed extract, 6-9 parts of cocklebur fruit extract, 3-6 parts of crocus sativus flower extract, 3-6 parts of scutellaria baicalensis root extract, 2-4 parts of actinidia arguta extract and 2-4 parts of snail secretion filtrate.

As a further preferred choice of the invention, the ceramide compound skin-activating factor also comprises 2-4 parts of yeast/rice fermentation product filtrate.

As a further preferred choice of the invention, the ceramide compound skin-activating factor for soothing the muscles is prepared by extracting camellia seeds, cranberry seeds, caraway seeds and grape seeds.

As a further preferable selection of the invention, the ceramide compound skin-activating factor for soothing the muscles comprises 1-2 parts of calendula oil.

As a further preferred choice of the invention, the ceramide compound skin-activating factor for soothing the muscles, the plant compound oil also comprises 1-2 parts of rice bran oil.

As a further preferred aspect of the present invention, the ceramide compound skin-activating factor for soothing the muscles is prepared by the following steps:

1) cleaning and drying the tea seeds, the cowberry seeds, the caraway seeds and the grape seeds according to a mass ratio of 2-3: 1.5-2.5: 1-2, uniformly mixing, and crushing to obtain mixed powder;

2) adding hydrolytic protease, xylanase, cellulase and deionized water into the mixed powder, mixing uniformly, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis for 30-45min at 35-45 ℃ to obtain a leaching solution;

3) inactivating enzymes in the leaching liquor obtained in the step 2), performing centrifugal separation at a high speed, and collecting upper-layer liquid;

4) adding extractant into the collected upper layer liquid, extracting for 2-3 times, and collecting the grease layer.

As a further preferred aspect of the invention, the ceramide compound skin-activating factor comprises 0.5-1.5% of the addition amount of the hydrolytic protease, 0.1-0.25% of the addition amount of the cellulase and 0.3-0.5% of the addition amount of the xylanase.

As a further preferred feature of the present invention, in the above ceramide compound anmei skin-activating factor, the extraction agent is diethyl ether.

The second technical scheme provided by the invention is as follows:

a cream containing ceramide compound skin-activating factor for soothing the muscles comprises, by 100% of the total mass of the cream, 2-5% of ceramide compound skin-activating factor and 95-98% of matrix.

As a further preferred aspect of the present invention, the above cream containing ceramide compound anmei skin-activating factor comprises, based on 100% of the total mass of the base, the following components by mass: 1-3.5% of potassium cetyl phosphate, 0.1-1% of sorbitan stearate and 0.3-1.2% of hydrogenated lecithin; 0.1-2% of cyclotetrasiloxane, 0.5-1% of cyclohexasiloxane, 0.8-2% of phytosterol oleate, 2-4% of cetearyl alcohol isononanoate, 1-3% of tetrahydro-methyl pyrimidine carboxylic acid, 0.02-0.1% of disodium EDTA (ethylene diamine tetraacetic acid), 3-8% of dipropylene glycol, 0.5-1% of Arabic gum, 0.5-0.8% of sodium hyaluronate and 3-6% of squalane; 0.5-1% of poly-gamma-sodium glutamate, 0.25-0.5% of carbomer, 0.2-0.6% of tocopheryl acetate, 0.5-2% of plant preservative and the balance of deionized water.

As a further preferable mode of the invention, the plant preservative is prepared by mixing chamomile essential oil, sage extract, magnolia flower extract and zanthoxylum fruit extract according to the mass ratio of 2-5:2-5:1-3: 1-2.

Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:

according to the technical scheme provided by the invention, the ceramide 1, the lupin seed extract, the platycladi seed extract, the cocklebur fruit extract, the crocus sativus flower extract, the scutellaria baicalensis root extract, the actinidia spinosa extract, the snail secretion filtrate and the like are scientifically formulated and synergized. Because the content of ceramide naturally exists in the skin and reaches as high as 40-50 percent, the technical scheme takes the ceramide as a main component, the ceramide is adhered to keratinocytes, the skin moisture is supplemented, the lupin extract and the lupin extract are rich in various proteins and amino acids and rich in anti-wrinkle vegetal peptides, the activity of metalloproteinase is high, the platycladi seed extract contains a large amount of fatty oil, the xanthium fruit extract mainly contains heterocyclic compounds such as volatile oil, fatty oil, phenolic acid, xanthium thiazine diketone and the like, anthraquinone, flavone and alkaloid, baicalin and baicalein, lactone and flavone in the scutellaria baicalensis extract, volatile oil, carotenoid and glycoside compounds thereof in the crocus sativus flower extract, carotene compounds, amino acids, glycoside compounds and soap of trimethylcyclohexene derivatives, flavone and glycoside compounds thereof; fruit acid, sugar, vitamins, actinidine and carotenoid in the kiwi fruit extract, and collagen, elastin, urea film essence, protein, vitamins and glycolic acid in the snail secretion filtrate, so that the effects of improving skin blood circulation, promoting skin metabolism, eliminating free radicals, inhibiting melanin deposition, deeply supplementing water and moisturizing, effectively promoting skin cell damage repair, repairing skin barrier, resisting aging, deeply nourishing skin and improving skin darkness are achieved.

2. According to the technical scheme provided by the invention, the brown rice fermentation filtrate is compounded, so that the nutritional ingredients required by the skin are further supplemented, the skin moisture loss is prevented, the defense capacity of the skin is increased, the damaged skin is calmed, the anti-inflammatory and anti-oxidation effects are effectively achieved, the skin is conditioned, the skin regeneration is promoted, the natural sebum membrane of the skin is repaired, the releasing capacity of the skin surface barrier is strengthened, the skin barrier is repaired, and the skin aging is delayed.

3. According to the technical scheme provided by the invention, the plant essential oil is extracted by enzyme-assisted ultrasonic waves, the extraction efficiency is high, effective nutrient components can be reserved to the greatest extent, the generation of cholesterol and ceramide is promoted, glutathione reductase is activated, collagen and cell layer fibronectin are improved, the connection part of epidermis and dermis is tightened, free radicals are eliminated and the like, so that the effects of activating skin, resisting aging and resisting oxidation are achieved.

4. According to the technical scheme provided by the invention, each component of the matrix is strictly screened and optimized, and double stimulation and damage to damaged skin are reduced.

5. The technical scheme provided by the invention adopts the plant preservative to inhibit gram-negative bacteria such as enterobacteria and the like, and gram-positive bacteria and fungi such as staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans, bacillus subtilis, streptococcus and the like, so that the damage to skin cells of a human body is minimized, no stimulation or anaphylactic reaction exists, and the plant preservative has biodegradability.

Detailed Description

The technical solution of the present invention is further described in detail with reference to the following embodiments, but the present invention is not limited thereto, and any limited number of modifications made by anyone within the scope of the present invention are still within the scope of the present invention.

It should be noted that the raw materials used in the technical scheme provided by the present invention, except for the specific description, are prepared by conventional means or purchased through commercial channels.

Example 1

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 127 parts of ceramide, 13 parts of plant compound oil, 8 parts of lupin seed extract, 11 parts of platycladi seed extract, 6 parts of cocklebur fruit extract, 6 parts of crocus flower extract, 3-6 parts of scutellaria root extract, 2 parts of actinidia arguta extract and 4 parts of snail secretion filtrate.

The vegetable composite oil is prepared by the following steps in sequence:

1) cleaning tea seeds, cowberry fruit seeds, caraway seeds and grape seeds, drying, uniformly mixing according to the mass ratio of 3:2:2.5:1, and crushing to obtain mixed powder;

2) adding 1.5% (w/w) of hydrolytic protease, 0.1% (w/w) of cellulase, 0.5% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis at 35 ℃ for 45min to obtain a leaching solution;

4) adding ether into the collected upper layer liquid, extracting for 2-3 times, extracting to collect oil layer, and evaporating to remove ether.

Example 2

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 120 parts of ceramide, 13 parts of plant compound oil, 8 parts of lupin seed extract, 11 parts of platycladi seed extract, 6 parts of cocklebur fruit extract, 6 parts of crocus flower extract, 3-6 parts of scutellaria root extract, 2 parts of actinidia arguta extract and 4 parts of snail secretion filtrate.

The vegetable composite oil is prepared by the following steps in sequence:

1) cleaning tea seeds, cowberry fruit seeds, caraway seeds and grape seeds, drying, uniformly mixing according to the mass ratio of 3:3:2:2, and crushing to obtain mixed powder;

2) adding 0.5% (w/w) of hydrolytic protease, 0.25% (w/w) of cellulase, 0.5% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis at 40 ℃ for 40min to obtain a leaching solution;

Example 3

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 120 parts of ceramide, 11 parts of plant compound oil, 10 parts of lupin seed extract, 10 parts of platycladi seed extract, 8 parts of cocklebur fruit extract, 5 parts of crocus flower extract, 4 parts of scutellaria baicalensis root extract, 3 parts of actinidia arguta extract, 3 parts of snail secretion filtrate and 4 parts of yeast/rice fermentation product filtrate.

The vegetable composite oil is prepared by the following steps in sequence:

1) cleaning tea seeds, cowberry fruit seeds, caraway seeds and grape seeds, drying, uniformly mixing according to the mass ratio of 3:3:1.5:1.5, and crushing to obtain mixed powder;

2) adding 1% (w/w) of hydrolytic protease, 0.2% (w/w) of cellulase, 0.4% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis for 30min at 45 ℃ to obtain a leaching solution;

Example 4

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 123 parts of ceramide, 11 parts of plant compound oil, 9 parts of lupin seed extract, 10 parts of platycladi seed extract, 7 parts of cocklebur fruit extract, 5 parts of crocus flower extract, 4 parts of scutellaria root extract, 3 parts of actinidia arguta extract and 2.5 parts of snail secretion filtrate.

The plant composite oil comprises 2 parts of calendula oil, 1 part of rice bran oil and 8 parts of composite oil prepared by the following steps in sequence:

1) cleaning tea seeds, cowberry fruit seeds, caraway seeds and grape seeds, drying, uniformly mixing according to the mass ratio of 2:2:2:2, and crushing to obtain mixed powder;

2) adding 1.2% (w/w) of hydrolytic protease, 0.2% (w/w) of cellulase, 0.4% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis at 42 ℃ for 35min to obtain a leaching solution;

4) adding ether into the collected upper layer liquid, extracting for 2 times, extracting the collected oil layer, and evaporating to remove ether.

Example 5

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 12 parts of ceramide, 12 parts of plant compound oil, 10 parts of lupin seed extract, 10 parts of platycladi seed extract, 7 parts of cocklebur fruit extract, 5 parts of crocus flower extract, 5 parts of scutellaria baicalensis root extract, 3 parts of actinidia arguta extract, 3 parts of snail secretion filtrate and 2 parts of yeast/rice fermentation product filtrate.

The plant composite oil comprises 2 parts of calendula oil, 2 parts of rice bran oil and 8 parts of composite oil prepared by the following steps in sequence:

1) cleaning tea seeds, cowberry fruit seeds, caraway seeds and grape seeds, drying, uniformly mixing according to the mass ratio of 3:3:1.5:1, and crushing to obtain mixed powder;

2) adding 1% (w/w) of hydrolytic protease, 0.25% (w/w) of cellulase, 0.5% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis at 38 ℃ for 38min to obtain a leaching solution;

Example 6

The invention provides a ceramide compound muscle-soothing and skin-activating factor which comprises the following components in parts by weight: 12 parts of ceramide, 12 parts of plant compound oil, 10 parts of lupin seed extract, 10 parts of platycladi seed extract, 7 parts of cocklebur fruit extract, 5 parts of crocus flower extract, 5 parts of scutellaria baicalensis root extract, 3 parts of actinidia arguta extract, 3 parts of snail secretion filtrate and 3 parts of yeast/rice fermentation product filtrate.

The plant composite oil comprises 1 part of calendula oil, 2 parts of rice bran oil and 9 parts of composite oil prepared by the following steps in sequence:

1) cleaning and drying the tea seeds, the cowberry seeds, the caraway seeds and the grape seeds according to a mass ratio of 2: 3: 2.5:1, uniformly mixing, and crushing to obtain mixed powder;

2) adding 1.2% (w/w) of hydrolytic protease, 0.2% (w/w) of cellulase, 0.5% (w/w) of xylanase and deionized water (the solid-to-liquid ratio is controlled to be 1g:6mL) into the mixed powder, uniformly mixing, adjusting to be alkaline, placing in an ultrasonic instrument, and carrying out enzymolysis at 45 ℃ for 32min to obtain a leaching solution;

Example 7

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 1% of example 5 ceramide compound anmei activating factor and 99% of matrix, wherein the total mass of the cream is 100%.

The matrix comprises the following components in percentage by mass based on 100% of the total mass of the matrix: 3% of squalane, 3.5% of potassium cetyl phosphate, 2% of cetearyl isononanoate, 3% of tetrahydro-methyl-pyrimidine carboxylic acid, 0.1% of sorbitan stearate and 1.2% of hydrogenated lecithin; 0.1% of cyclotetrasiloxane, 1% of cyclohexasiloxane, 0.8% of phytosterol oleate, 0.1% of EDTA disodium, 3% of dipropylene glycol, 1% of Arabic gum and 0.5% of sodium hyaluronate; 1% of poly gamma-sodium glutamate, 0.25% of carbomer, 0.6% of tocopheryl acetate, 0.5% of plant preservative and the balance of deionized water.

The plant preservative is prepared by mixing chamomile essential oil, a sage extract, a magnolia flower extract and a zanthoxylum fruit extract according to a mass ratio of 5:5:1: 2.

Example 8

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 5% of example 5 ceramide compound anmei activating factor and 95% of matrix, wherein the total mass of the cream is 100%.

The matrix comprises the following components in percentage by mass based on 100% of the total mass of the matrix: squalane 6%, potassium cetyl phosphate 1%, cetearyl isononanoate 4%, tetrahydro-methyl-pyrimidinecarboxylic acid 1%, sorbitan stearate 1%, hydrogenated lecithin 0.3%; 2% of cyclotetrasiloxane, 0.5% of cyclohexasiloxane, 2% of phytosterol oleate, 0.02% of disodium EDTA, 8% of dipropylene glycol, 0.5% of Arabic gum and 0.8% of sodium hyaluronate; 0.5% of poly-gamma-sodium glutamate, 0.5% of carbomer, 0.2% of tocopheryl acetate, 2% of plant preservative and the balance of deionized water.

The plant preservative is prepared by mixing chamomile essential oil, a sage extract, a magnolia flower extract and a zanthoxylum fruit extract according to the mass ratio of 2:2:2: 2.

Example 9

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 3% of ceramide compound anmei activating factor in example 1 and 97% of matrix, wherein the total mass of the cream is 100%.

The matrix comprises the following components in percentage by mass based on 100% of the total mass of the matrix: 4% of squalane, 3% of potassium cetyl phosphate, 3% of cetearyl isononanoate, 2% of tetrahydro-methyl-pyrimidinecarboxylic acid, 0.5% of sorbitan stearate and 0.8% of hydrogenated lecithin; 0.8% of cyclotetrasiloxane, 0.6% of cyclohexasiloxane, 1.5% of phytosterol oleate, 0.05% of disodium EDTA (ethylene diamine tetraacetic acid), 5% of dipropylene glycol, 0.8% of Arabic gum and 0.6% of sodium hyaluronate; 0.8% of poly-gamma-sodium glutamate, 0.35% of carbomer, 0.4% of tocopheryl acetate, 1% of plant preservative and the balance of deionized water.

The plant preservative is prepared by mixing chamomile essential oil, a sage extract, a magnolia flower extract and a zanthoxylum fruit extract according to a mass ratio of 2:5:1: 2.

Example 10

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 3% of example 3 ceramide compound anmei activating factor and 97% of matrix, wherein the total mass of the cream is 100%; the matrix was identical in composition and content to the matrix in example 9.

Example 11

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 3% of example 4 ceramide compound anmei activating factor and 97% of matrix by the total mass of the cream being 100%, wherein the matrix is consistent with the components and the content of the matrix in example 9.

Example 12

The invention provides a cream containing ceramide compound anmei activating factor, which is prepared from 3% of example 5 ceramide compound anmei activating factor and 97% of matrix by the total mass of the cream being 100%, wherein the matrix is consistent with the components and content of the matrix in example 9.

The ceramide polypeptide compound Anmyo skin-activating cream described in examples 7 to 12 is prepared according to the conventional process in the field; and evaluating the safety of the prepared ceramide polypeptide compound skin-activating cream for soothing the muscles.

(1) Microbiological test item experiments: according to the determination method of the total bacterial colony number, faecal coliform group, staphylococcus aureus, pseudomonas aeruginosa, mould and saccharomycetes in the cosmetic hygiene standard, the microorganism detection of the ceramide polypeptide compound skin-activating cream provided by the invention is qualified.

(2) And (3) testing sanitary chemical inspection projects: according to the determination method of total mercury, total arsenic and total lead in the cosmetic hygiene standard and the stipulation thereof, the metal element detection of the ceramide polypeptide compound skin-activating cream for soothing the muscles provided by the invention is all qualified.

(3) Skin irritation/corrosivity test: according to the skin irritation/corrosivity classification standard in the cosmetic hygiene standard, the ceramide polypeptide compound skin-activating cream provided by the invention has no irritation.

To better demonstrate the efficacy of the present invention, the efficacy experiments of the present application, as well as the efficacy experiments of the comparative examples, are given below:

comparative example 1

The other components were in the same amounts as in example 12, except that the same amount of cocklebur fruit extract was used instead of the ceramide in example 12.

Comparative example 2

The other components were in the same amounts as in example 12, except that the same amount of the platycladi seed extract was used instead of the scutellaria root extract in example 12.

Comparative example 3

The other components were in the same amounts as in example 12, except that the same amount of crocus sativus flower extract was used instead of the actinidia arguta extract in example 12.

Comparative example 4

The other components and contents are the same as those in example 12, except that the vegetable composite oil is prepared by separately purchasing catechu seed oil, cowberry seed oil, caraway seed oil and grape seed oil in a mass ratio of 3:3:1.5:1.

Comparative example 5

The other ingredients were the same as in example 12 except that phenoxyethanol/ethylhexylglycerin (0.5%) was used in place of the plant preservative in example 1 and the remainder was supplemented with deionized water.

1. Moisture retention test:

the specific test method is as follows:

reference standard: refer to QB/T4256-.

The testing personnel: 30 healthy volunteers were selected, and the ages were 30-40 years old, respectively.

And (3) testing conditions are as follows: the capacitance method skin moisture tester Corneometer CM825 is used for testing the temperature of 20-22 ℃ and the humidity of 40-60%.

The test principle is as follows: the capacitance method is used for measuring the moisture content of the human skin stratum corneum, and is based on the obvious difference of dielectric constants of water and other substances, the capacitance values of the measured skin are different according to the moisture content of the skin stratum corneum, and the parameters can represent the moisture content (MMV) of the skin.

Test samples: the experimental groups were the products provided in examples 9-12 and the control groups were the products provided in comparative examples 1-5.

The test method comprises the following steps: each area is fixedly measured at three points according to a certain sequence, and an average value of the three points is obtained. Then, the ceramide polypeptide compound skin-activating ointment for soothing the muscles is smeared on a tested part (2.0 +/-0.1) mg/cm²The amount of (c) was applied in a single application), the skin moisture content of the test site was measured at 0h, 1h, 4h and 8h using a probe, and the average value of each group was calculated for the corresponding time point, and the results are shown in table 1.

The skin moisture content increase rate calculation formula is as follows:

rate of increase in skin moisture content％＝(MMVt-MMV₀)/MMV₀×100％

MMV₀MMV of skin before application

MMV_t-MMV of skin t time period after application

The results are shown in Table 2.

TABLE 2

2. Skin elasticity test

The experimental method comprises the following steps: the testing personnel: 45 healthy volunteers aged 30-40 years respectively were selected and randomly grouped into 5 groups. The samples were applied twice daily in the morning and evening. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site.

Skin elasticity test: the skin condition at week 0 and week 4 was measured using the skin elastometer MPA580, the test site was the cheekbone, the probe repeated 3 times in the same test area during the measurement, and the average was taken, and the test results are shown in Table 3:

TABLE 3

3. DPPH free radical scavenging test

0.2mmol/L DPPH solution is prepared by 50% ethanol and stored away from light. In the experimental group, 0.2mg/mL of solution to be tested is prepared from the products provided in examples 9-12 and the comparison group 1-5, the absorbance A of the solution to be tested is measured at 517nm, the absorbance A is measured in parallel for 3 times, the average value is taken, and the experimental result is shown in Table 3.

In the formula: a. the_iIs the absorbance of the reaction after addition of the test substance;

A_jthe absorbance of the test solution is the self absorbance of the test solution;

A₀the blank value is the blank value without test solution.

TABLE 3

4: skin roughness test

1) Test samples: the experimental groups were the products provided in examples 9-12 and the control groups were the products provided in comparative examples 1-5.

2) The test method comprises the following steps: 90 wrinkled female volunteers with the average age of 30-40 years old were taken, randomly divided into 9 groups of 10, and the above test samples were used respectively. The subject takes a proper amount of cream to apply on the face, and the cream is applied once every morning and evening for 28 days continuously. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site.

Data were collected on day 0 and day 28 using a skin wrinkle tester vision meter SV600, and the test site was the zygomatic region. The test parameter is skin roughness R (the difference between the highest peak and lowest valley of the skin lines), the greater the value of R, the rougher the skin and the more severe the wrinkles. The test results are shown in table 4.

Table 4 skin roughness test results

As is apparent from tables 1 to 4, the technical scheme provided by the invention can deeply penetrate into the bottom layer of the skin to supplement moisture, keep moisture for a long time, effectively remove free radicals, recover the elasticity of the skin, promote the skin to recover a good barrier function, effectively lock moisture, carefully nourish the skin, and have good skin-care and anti-aging effects.

Human body trial

1. The test population: 100 people, age 30-40 years.

2. Test samples: by using the product provided in example 12, trial and civil investigation statistics are performed, and experience statistics on various senses such as moisture retention, skin-activating feel, repairing feel, irritation and the like are performed, wherein 96% of the results are satisfied with the moisture retention and the moisture retention, 96% of the results are satisfied with the soothing feel, 97% of the results are satisfied with the repairing feel, 98% of the results are considered that the product provided by the present application has good skin-activating ability, 100% of the results do not have any irritation, and testers are satisfied with the overall evaluation of the product.

Claims

1. A cream containing ceramide compound skin-activating factor for soothing the muscles is characterized in that the cream is prepared from 2-5% of ceramide compound skin-activating factor and 95-98% of matrix by the total mass of the cream being 100%;

the matrix comprises the following components in percentage by mass based on 100% of the total mass of the matrix: 3-6% of squalane, 1-3.5% of potassium cetyl phosphate, 2-4% of cetearyl isononanoate, 1-3% of tetrahydro-methyl pyrimidine carboxylic acid, 0.1-1% of sorbitan stearate and 0.3-1.2% of hydrogenated lecithin; 0.1-2% of cyclotetrasiloxane, 0.5-1% of cyclohexasiloxane, 0.8-2% of phytosterol oleate, 0.02-0.1% of disodium EDTA (ethylene diamine tetraacetic acid), 3-8% of dipropylene glycol, 0.5-1% of Arabic gum and 0.5-0.8% of sodium hyaluronate; 0.5-1% of poly gamma-sodium glutamate, 0.25-0.5% of carbomer, 0.2-0.6% of tocopheryl acetate, 0.5-2% of plant preservative and the balance of deionized water; the plant preservative is prepared by mixing chamomile essential oil, a sage extract, a magnolia flower extract and a zanthoxylum fruit extract according to the mass ratio of 2-5:2-5:1-3: 1-2;

the ceramide compound muscle-soothing and skin-activating factor comprises the following components in parts by weight: 120-27 parts of ceramide, 10-13 parts of plant compound oil, 8-11 parts of lupin seed extract, 8-11 parts of platycladi seed extract, 6-9 parts of cocklebur fruit extract, 3-6 parts of crocus sativus flower extract, 3-6 parts of scutellaria baicalensis root extract, 2-4 parts of actinidia arguta extract and 2-4 parts of snail secretion filtrate;

the plant composite oil is mainly prepared by extracting camellia seed, cranberry seed, caraway seed and grape seed sequentially through the following steps:

2. The cream containing the ceramide compound anmei activating factor is characterized in that the ceramide compound anmei activating factor also comprises 2-4 parts of yeast/rice fermentation product filtrate.

3. The cream containing the ceramide compound anmei skin-activating factor as claimed in claim 1, wherein the plant compound oil in the ceramide compound anmei skin-activating factor further comprises 1-2 parts of calendula oil.

4. The cream containing the ceramide compound anmei skin-activating factor as claimed in claim 3, wherein the plant compound oil in the ceramide compound anmei skin-activating factor further comprises 1-2 parts of rice bran oil.

5. The cream containing ceramide compound anmei activating factor is characterized in that the addition amount of the hydrolytic protease is 0.5-1.5%, the addition amount of the cellulase is 0.1-0.25%, and the addition amount of the xylanase is 0.3-0.5%.

6. The cream containing ceramide compound anmeihuo factor as claimed in claim 1, wherein the extractant is diethyl ether.