JP2000319122A - Cell proliferation promoting cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic having cell proliferation promoting action and effective for preventing and improving undesirable changes accompanying to the aging of the skin such as wrinkles, spots, dull skin and dry skin. SOLUTION: The objective cosmetic utilizes a cell proliferation promoting action developed by the combined use of soybean extract liquid, glabridin and fruit extract liquid. Especially effective fruit extract liquids are those produced by using citrus fruits, jujube, haw, grape, apple or strawberry as a raw material.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having an action of promoting the proliferation of epidermal cells and preventing aging of the skin.

More specifically, the present invention relates to a cell proliferation promoting cosmetic which is effective in preventing and improving wrinkles, dark spots, dry skin and spots caused by aging of the skin, and which is excellent in safety.

[0003]

2. Description of the Related Art Human skin gradually ages with aging, and it is known that various external stimuli other than aging cause skin aging. Especially ultraviolet rays in sunlight, collagen, a component of the extracellular matrix,
It causes cross-linking and cutting of elastin and the like, thereby reducing the elasticity and moisturizing property of the skin, causing wrinkles, dry skin, roughness and the like. The skin that has undergone such a change becomes more susceptible to external stimuli, falls into a vicious cycle of reduced cellular activity and reduced regenerative power of the extracellular matrix, and aging proceeds rapidly. The disappearance of skin gloss, increase of spots, dark spots, etc., which progresses in a short period of time, is often caused by the strong external stimulus as described above.

[0004] Therefore, in order to prevent skin aging due to external irritation, it has been practiced to add an ultraviolet absorber or a humectant to cosmetics. In addition, it has been attempted to mix a component that inhibits collagenase and elastase in cosmetics to maintain the extracellular matrix in a normal state, and to mix components that promote cell growth such as vitamin C and vitamin E. Have been.

[0005] However, it is difficult to completely block harmful ultraviolet rays with an ultraviolet absorber in an amount that can be blended normally, and if it is blended in a large amount, irritation to the skin becomes a problem. It is also difficult to sufficiently activate skin cells with a humectant, a collagenase inhibitor or the like, and cell growth promoting components such as vitamin C have problems in safety, stability and the like, and have not always been satisfactory.

[0006]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a cosmetic composition having a cell growth promoting effect which is effective for preventing and improving undesired changes due to skin aging, such as wrinkles, spots, dark spots, and dry skin. To provide a fee.

[0007] Another object of the present invention is to provide a cosmetic composition which contains a component derived from a natural product having high safety and has an effect of preventing and improving skin aging.

[0008]

Means for Solving the Problems The cosmetic of the present invention which has successfully achieved the above object contains a soybean extract, a glabridine and a fruit extract, and has a synergistic action of these components to promote cell growth. It is characterized by having.

[0009] In addition to the above-mentioned three components, those containing one or more plant extracts selected from the group consisting of pentagon extract, carrot extract and safflower extract,
It was also confirmed that the cosmetic had a more excellent cell growth promoting action.

[0010] Here, the "fruit extract" includes not only an extract obtained by subjecting a fruit to an extraction treatment with a solvent, but also a juice obtained by squeezing a multi-juice fruit.

[0011] In addition, "Ogon extract" is Ogon (S
cutellaria basicis Ge
orgi. ) And “Carrot extract” refers to carrot (Panax ginseng CA Meyer)
Roots, "safflower extract" is safflower (Cartha
mus tinctorius L. ) Are obtained by extracting flowers with aqueous alcohols such as aqueous ethanol and aqueous 1,3-butylene glycol.

The "cell growth promoting effect" is an effect confirmed by the test method described below.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION The soybean extract used in the present invention is:
Soybeans (Glycine max Merrill) with hydrous alcohol (for example, hydrous ethanol, hydrous 1,3-butylene glycol, hydrous propylene glycol, hydrous glycerin, etc.) having an alcohol concentration of about 30 to 70% by weight.
Is a saponin-containing extract obtained by extraction treatment. In the case of an extract using a solvent which can be mixed in the cosmetic, the extract can be used as it is, or a concentrated or diluted extract can be added to the cosmetic as needed. The extract may be used as a dry solid material and then redissolved in the manufacture of cosmetics.

Gravlidine is a licorice (Glycyrrh)
iza grabra L. ) Is a type of flavonoid contained in the root. For this compound,
It is known to have a whitening action, an antioxidant action and an antibacterial action. Since the extract (so-called oil-soluble licorice extract) obtained by extracting licorice root with ethanol, acetone, ethyl acetate or the like is contained in a large amount, this extract may be used as it is.

As the fruit extract used in combination with the above two components, those obtained from citrus fruits and many other fruits can be used. Specific examples of suitable fruits as raw material fruits include orange, lemon, In addition to citrus fruits such as lime, grapefruit, Unshu mandarin orange and yuzu, there are jujube, hawthorn, grape, apple, strawberry and the like.

It is appropriate to use an extract with a hydrophilic solvent for fruits which are difficult to extract by squeezing, such as jujube and hawthorn fruits. Juice obtained from juice may be used. As the solvent used for the extraction, the same hydrous alcohol as the solvent used for producing the soybean extract is suitable.

The cell growth-promoting action of the cosmetic of the present invention first appears when a soybean extract, glabridine and certain fruit extracts are used in combination. In this case, it has been empirically found that the fruit extract effective for exhibiting the cell growth promoting action is often based on fruits having a high content of organic acids and sugars, such as the fruits exemplified above. However, the requirements for the fruit extract to be effective have not been fully elucidated. Therefore,
When the fruit extract for carrying out the present invention is to be selected from those other than the above-mentioned ones (or when the above-mentioned ones and the other ones are to be used in combination), the soybean extract and the glabridine are used. It is desirable to select after confirming the effectiveness based on the results of an experiment for confirming the cell growth promoting action when used in combination.

The combined use of a plurality of fruit extracts is preferred in many cases because it is effective for enhancing the activity.

The preferred combination ratio (weight ratio) of the above-mentioned active ingredients in the cosmetic of the present invention is as follows.
When it is set to 0, it is as follows. Soybean extract: 100 to 5,000 as solids Fruit extract: 10 to 1,000 as solids For cosmetics, these three components in a total amount of 0.001
It is desirable to add so as to be about 2.0% by weight.

In the case where the extract of the pentagon, the extract of the carrot or the extract of the safflower are also blended, the addition rate of these extracts is about 0.
It is desirable that the content be 0001 to 0.5% by weight.

The cosmetic for promoting cell proliferation of the present invention takes the form of any cosmetic applied to the skin, such as lotions, emulsions, creams, packs, gel cosmetics, powder cosmetics, soaps, and liquid detergents. be able to.

When producing the cosmetic of the present invention, the components usually used in the production of the cosmetic, such as oils, surfactants, thickeners, humectants, preservatives, fragrances, coloring agents, chelating agents , An amino acid, a saccharide, etc., and an active ingredient peculiar to the present invention. If necessary, any physiologically active substance, for example, glycyrrhizic acid and its salts, glycyrrhetinic acid and its derivatives (such as stearyl glycyrrhetinate), allantoin which is an anti-inflammatory agent , Azulene, baicalin, baicalein, marronnier extract, oak extract, spinach extract, etc .; whitening agents such as ascorbic acid, magnesium ascorbate phosphate, etc., ellagic acid, arbutin, kojic acid, placenta extract, mulberry bark extract , Jinjin extract,
Waremokou extract, Age extract, etc .; vitamins, antioxidant tocopherol and its derivatives, natural vitamin E; animal components such as collagen, elastin, hyaluronic acid, chondroitin sulfate, etc. are blended and manufactured by a conventional method. be able to.

[0023]

The present invention will be described below with reference to examples. The components specific to the present invention used in each example are as follows.

Soybean extract: hydrated 1,3-butylene glycol extract of crushed soybean (solids concentration: 0.35% by weight) Gravlidine: licorice ethanol extract containing 40% by weight of glabridine Jujube extract: jujube fruit Water-containing 1,3-butylene glycol extract (solid content: 5.5%) Hawthorn extract: water-containing 1,3-butylene glycol extract of hawthorn fruit (solid content: 1.0%)

Grapefruit extract: 50% 1,3-butylene glycol aqueous solution 1
An extract obtained by adding 0 kg, allowing to stand for 7 days, and then filtering. 2.3% solids concentration.

Apple extract: Extracted in the same manner as the above grapefruit extract. 1.8% solids concentration.

Orange juice: 10 kg of a 50% 1,3-butylene glycol aqueous solution is added to a juice obtained by squeezing 1 kg of a crushed raw material fruit with a squeezing-type squeezing machine for preservation.
And obtained by filtration. 1.8% solids concentration.

Lemon juice: obtained in the same manner as the above orange juice. Lime juice having a solid content of 2.1%: obtained in the same manner as the orange juice. 1.7% solids content Yuzu juice: obtained in the same manner as the above orange juice. Grape juice with a solid content of 1.5%: A juice obtained in the same manner as the orange juice. 2.3% solids content Strawberry juice: obtained in the same manner as the above orange juice. Solid concentration 1.4%

Examples 1 to 4 and Comparative Examples 1 to 3 A lotion was produced from the following A to C by a conventional method. (A) Dipropylene glycol 4% by weight 1,3-butylene glycol 2 Polyoxyethylene (60) hydrogenated castor oil 1 Ethyl alcohol 4 Methyl parahydroxybenzoate 0.1 Citric acid 0.2 Sodium citrate 0.1 Perfume 02 (B) See Table 1 (C) Water balance

[0030]

Table 1 B component Example 1: Soybean extract (0.105%), glabridine (0.01%), jujube extract (0.055%), lime juice (0.017%) Ingredient of Example 1 + safflower extract (0.03
%) Example 3: Soybean extract (0.0625%), glabridine (0.005%), orange juice (0.0018)
%), Lemon juice (0.0021%), grapefruit juice (0.0023%), apple juice (0.0018%)
%) Example 4: Ingredient of Example 3 + carrot extract (0.00%)
Comparative Example 1: Excluding glabridine from the components of Example 1 Comparative Example 2: Excluding the soybean extract from the components of Example 1 Comparative Example 3: Components of Example 1 Jujube extract and lime juice are more excluded (Note: Although the licorice extract was actually used as glabridine, the indicated addition rate is the addition rate as glabridine. The same applies to each of the following examples.)

Test Example 1 The lotions of Examples 1 to 4 and Comparative Examples 1 to 3 were tested for cell growth promoting activity by the following MTT method.

The MTT method (J. Immunol. Meth)
od 93,157,1986): Pre-cultured human normal neonatal dermal fibroblasts (NB1RGB) were dispensed into a 96-well microplate and 10% FB in which the sample was dissolved
RITC80-7 medium supplemented with S culture medium (Kyokuto Pharmaceutical Co., Ltd.
pH 7.2) and culture at 37 ° C. under 5% CO ₂ for 3 days. Then, the MTT reagent (3- (4,5-dim)
thy1-2-thiazole1) -2,5-dip
heny1-2H tetrazoliumbromi
Add 20 μl of de, 5 mg / ml) and incubate for 4.5 hours (reactive oxygen from mitochondria in the proliferating cells reacts with the MTT reagent, and the color of the yellow reagent has an absorption peak at 570 nm) It turns blue-yellow.) Thereafter, the absorbance (A) at 570 nm is measured using a microplate reader. Separately, the same culture is performed without adding a sample, and the absorbance (B) is measured. Furthermore, the absorbance (C) when the same treatment is performed without adding the sample and without the cells is measured, and the absorbance (D) when the same treatment is performed without adding the sample and the cell is also measured. Each absorbance measurement corrects the effect of increasing turbidity due to the grown cells by subtracting the absorbance at 650 nm measured simultaneously. From the corrected absorbances, the cell growth promotion rate is determined by the following equation.

Cell growth promotion rate (%) = [｛(AC) −
(BD)｝ / (BD)] × 100

The results are shown in Table 2. The cell growth promoting effect was confirmed for the lotions of all Examples, and particularly excellent cell growth promoting effects were confirmed for the lotions of Examples 2 and 4. . However, the lotions of Comparative Examples 1 to 3 were all ineffective.

[0035]

[Table 2]

Example 5 A cream was produced by treating the following ingredients in a conventional manner. Cell growth promoting substance Soybean extract 0.35% by weight Gravlidine 0.03 Carrot extract 0.065 Oregon extract 0.012 Safflower extract 0.0075 Orange juice 0.09 Jujube extract 0.055 Grapefruit extract 0.0 023 Cetostearyl alcohol 3.5 Squalane 30.0 Beeswax 3.0 Reduced lanolin 5.0 Stearyl paraoxybenzoate 0.1 Polyoxyethylene (20) sorbitan monostearate 2.0 Glycerin monostearate 2.0 1,1 3-butylene glycol 5.0 Perfume 0.05 Purified water Remainder

COMPARATIVE EXAMPLE 4 A cream was prepared by removing the eight cell growth promoting substances from the raw materials of Example 5.

Test Example 2 18 women (age 3) complaining of skin wrinkles, wrinkles, and xerostomia
The following use tests were performed on the creams of Example 5 and Comparative Example 4 using, as panelists, 3 to 45 years old;

Test method: The panelists were divided into two groups of nine persons, one of which received the cream of Example 5 and the other of which received the cream of Comparative Example 4 twice daily for 50 days, every morning and evening.
It was applied to the face. Fifty days later, the number and depth of gloss, blemishes and squats, and wrinkles on the coated surface were visually observed, and the scores were answered according to the following criteria.

[Skin Gloss, Matte and Kasatsu] Score Observation Result 1 There is no skin gloss, and the skin is strong. Exfoliation of the stratum corneum is often observed. 2 There is no skin gloss, there is dark spots on the whole, and exfoliation of the stratum corneum is seen in some places. 3 The skin is slightly shiny and no dark spots are seen. The stratum corneum is not peeled off with the naked eye, but it comes off when lightly rubbed with cotton wool. 4 The skin is shiny and firm. Little peeling of the stratum corneum even when rubbed with cotton wool.

[Wrinkle] Score Observation Result 1 Many wrinkles are found at the corners of the eyes. 2 There are wrinkles at the corners of the eyes, and the number is slightly large. 3 There are wrinkles at the corners of the eyes, but the number is small. 4 No wrinkles at the corners of the eyes are observed with a normal expression.

The test results are shown in Tables 3 and 4.

[0043]

[Table 3]

[0044]

[Table 4]

Example 6 An emulsion having the following composition was produced by a conventional method. Soybean extract 0.0625% by weight Gravlidine 0.02 Lemon juice 0.0042 Jujube extract 0.0055 Yuzu juice 0.006 Hawthorn extract 0.02 Carrot extract 0.065 Cetyl alcohol 0.5 Squalane 3.0 Beeswax 1.0 Vaseline 2.0 Polyoxyethylene (20) sorbitan monooleate 1.0 Glycerin monostearate 1.0 Dipropylene glycol 4.0 Ethanol 2.0 Methyl parahydroxybenzoate 0.12 Sodium hyaluronate 0. 1 Remaining purified water

Example 7 A pack having the following composition was produced by a conventional method. Soybean extract 0.07% by weight Gravlidine 0.03 Ougon extract 0.05 Safflower extract 0.08 Grapefruit juice 0.008 Grape juice 0.003 Strawberry juice 0.008 Polyvinyl alcohol 15.0 Polyethylene glycol 3.0 Propylene Glycol 7.0 Ethanol 10.0 Methyl paraben 0.05 Perfume 0.05 Purified water Remainder

Example 8 An ointment having the following composition was produced by a conventional method. Soybean extract 0.05% by weight Gravlidine 0.02 Carrot extract 0.03 Lime juice 0.0055 Lemon juice 0.008 Orange juice 0.005 Jujube extract 0.003 Cetyl alcohol 18.0 Mokurou 20.0 Polyoxy Ethylene (20) monooleate 0.25 Glycerin monostearate 0.25 Vaseline 40.0 Purified water balance

Claims (3)

[Claims] 1. A cosmetic comprising a soybean extract, glabridine and a fruit extract, which has a cell growth promoting action. 2. It contains a soybean extract, glabridine and a fruit extract, and further contains one or more plant extracts selected from the group consisting of a gougon extract, a carrot extract and a safflower extract, A cosmetic having a cell growth promoting action. 3. The cosmetic according to claim 1, wherein the fruit extract is an extract of one or more fruits selected from the group consisting of citrus, jujube, hawthorn, grape, apple, and strawberry. .