CN113116798A

CN113116798A - Face cream containing anti-aging composition and preparation method thereof

Info

Publication number: CN113116798A
Application number: CN202110420193.6A
Authority: CN
Inventors: 龚德明; 容惠; 邹小灵; 徐洁琼; 胡桂林
Original assignee: Guangdong Fanmilin Biotechnology Co Ltd
Current assignee: Guangdong Fanmilin Biotechnology Co Ltd
Priority date: 2021-04-19
Filing date: 2021-04-19
Publication date: 2021-07-16

Abstract

The invention provides a face cream containing an anti-aging composition and a preparation method thereof. The raw materials for preparing the cream containing the anti-aging composition comprise the anti-aging composition, a skin conditioner, an emulsifier, a humectant and water; the anti-aging composition comprises a ginger root extract, a scutellaria root extract and a cacao seed extract; the skin conditioner comprises carnosine, ferulic acid, adenosine and chlorella extract. The anti-aging composition and the skin conditioner disclosed by the invention have synergistic interaction of all components, can effectively inhibit the generation of AGEs, reduce glycosylation reaction, can obviously improve dark yellow skin after long-term use, and can effectively enhance the skin resistance, improve the skin elasticity, reduce wrinkles and keep the skin in a glossy and fine healthy state.

Description

Face cream containing anti-aging composition and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetic production and application, and particularly relates to a cream containing an anti-aging composition and a preparation method thereof.

Background

Cellular senescence is a process by which cells enter a permanent cell cycle block. Cellular senescence is often associated with a number of pathological features, of which inflammation is one. Among them, the concept of inflammatory aging was first proposed in 2000, and inflammatory aging became a new direction in aging research. Normal inflammatory response is a non-specific immune response designed to protect humans from life-causing injury, and excessive inflammatory response is involved in the pathological processes of many aging-related diseases in addition to skin aging. The immune system in normal organism can recognize and remove senescent cells, and the blast cells in the tissue are differentiated to form young cells to replace the senescent cells, but as the organism ages and damages, the cell aging is accelerated, and the removal speed of the senescent cells by the immune system is slowed down, so that the senescent cells in the organism are increased.

Dark yellow skin color is one of the clinical manifestations of skin aging. Internal factors are related to the deficiency of five internal organs, disorder of qi and blood, stagnation of turbid phlegm and the like, and the most closely related to the dysfunction of the kidney, spleen and liver. External factors: aging of free radicals, non-enzymatic glycosylation, carbonyl stress. The glycation reaction is generated by the human body, the metabolism of the human body is slowed down along with the aging, sugar ingested in the human body is easy to accumulate and then is combined with protein for oxidation to finally form AGEs, and the substances can crosslink collagen, reduce the solubility and the permeability of the collagen, cause the reduction of the elasticity of the skin and cause the aging.

CN107468585A discloses a composition for improving dark and yellowish complexion, a preparation method thereof and application thereof in cosmetics. Comprises the following components by weight: 5-13 parts of artemisia argyi extract, 7-13 parts of sargassum elongatum extract, 3-7 parts of sodium hyaluronate, 0.5-5.5 parts of glucosyl hesperidin, 1-3 parts of nano titanium dioxide, 8-12 parts of humectant, 5-10 parts of emulsifier, 0.1-0.9 part of thickener, 0.6-1 part of preservative, 0.8-5 parts of surfactant and a proper amount of pH value regulator, and water is added to 100 parts. The composition for improving the dark and yellow complexion can sweep out the dark and yellow complexion, but mainly aims at simple moisturizing and repairing, and neglects the prevention of the early aging.

CN110693778A discloses a cream, wherein phase a comprises a coffea arabica seed extract; phase B comprises rice bran oil, oleum Cocois, shea butter and olive fruit water; phase C contains triethanolamine; the phase D comprises carnosine; the phase E comprises acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; phase F contains Palmaria Oblonga pulp extract, Gentiana lutea root extract, Sophora flavescens root extract, Glycyrrhiza inflate root extract, Scutellaria baicalensis root extract, yerba mate leaf extract, Dunaliella salina extract, yeast lysate extract, palmitoyl hexapeptide-12, acetyl heptapeptide-4, and Vaccinium myrtillus fruit extract, which are also maintained after aging production, but neglects prevention of incipient aging.

Therefore, the development of a skin care product with high anti-inflammatory, anti-glycation and moisturizing effects is the focus of research in the field.

Disclosure of Invention

In view of the defects of the prior art, the invention aims to provide a face cream containing an anti-aging composition and a preparation method thereof. The cream containing the anti-aging composition has the effects of resisting inflammation, resisting saccharification and moistening and repairing.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a cream containing an anti-aging composition, which is prepared from an anti-aging composition, a skin conditioner, an emulsifier, a humectant and water;

the anti-aging composition comprises a ginger root extract, a scutellaria root extract and a cacao seed extract;

the skin conditioner comprises carnosine, ferulic acid, adenosine and chlorella extract.

In the invention, the anti-aging composition improves the skin tolerance by regulating the redox balance of cells, regulating cell autophagy and promoting cell detoxification, and can be matched with each component in a skin conditioner of cream to realize synergistic interaction, so that the anti-inflammatory, anti-oxidation and energy-energizing and anti-compression effects of the cream are improved.

Wherein, the ginger flower root extract can regulate autophagy of fibroblast, thereby realizing metabolism requirement of cells, update of certain organelles and maintain cell homeostasis; even under the blue light pressure, the lysosome network of the fibroblast can be protected, the skin is helped to improve the natural protective ability of the skin, so that the blue light/UV injury can be resisted, the cell detoxification can be promoted, the skin regeneration can be promoted, and the like. The radix Scutellariae extract has effects of inhibiting elastase, interfering secretion, and scavenging free radicals, thereby increasing skin metabolism, skin elasticity and wrinkle resistance, and resisting aging; promoting cerebral amide, improving sebum, and moistening skin; has effects in inhibiting dermatitis, enhancing skin resistance, and resisting inflammation; can neutralize released histamine, and has effect in preventing allergy. The cocoa seed extract is rich in molecular peptide, saccharide and polyphenol, reduces active oxygen under the blue light pressure, protects opsin photoreceptor under the blue light pressure, increases the content of collagen I, fibrillarin-1 and syndecan-4, and obviously improves the fiber network structure of elastin, thereby improving the skin elasticity and obviously improving the skin wrinkles.

Among them, carnosine is capable of inhibiting lipid oxidation catalyzed by metal ions, hemoglobin, lipase and active oxygen. Carnosine is a natural anti-glycosylation agent that is easily glycosylated with aldoses and ketoses and protects proteins against glycosylation and cross-linking by MDA and HOCL. The inhibition rate of ferulic acid on CML and fluorescent AGEs generated by the system reaches 90%. The ferulic acid has good ultraviolet absorption near 290-330 nm, the ultraviolet of 305-315 nm is most likely to induce skin erythema, and the ferulic acid and the derivatives thereof can reduce the cytotoxic effect of high-dose UVB on melanocytes and play a certain photoprotection effect on epidermis. Adenosine is a building block of cyclic adenosine monophosphate (cAMP), an important second messenger in signaling, and is involved in a variety of biological processes; can realize various functions of regulating cell proliferation by releasing metabolically active cells into microenvironment. Chlorella vulgaris extract is derived from green algae in the blue Niyowa of Japan, is rich in amino acids and trace elements, especially lutein, and has effects of resisting chronic inflammatory aging, promoting neogenesis, and promoting DEJ barrier repair at epidermis-dermis junction; the elastic collagen is added, so that the aging signs of the skin are obviously reduced, the skin texture is smoothed, and the skin is softer, smoother and more delicate; reducing the number and area of wrinkles.

Preferably, in the anti-aging composition, the mass ratio of the ginger flower root extract, the scutellaria root extract and the cacao seed extract is (3-8): (5-15): (2-7);

wherein "3-8" can be 3, 4, 5, 6, 7, 8, etc.;

wherein, 5-15 can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, etc.;

wherein "2-7" may be 2, 3, 4, 5, 6, 7, etc.

Preferably, in the skin conditioner, the mass ratio of the carnosine, the ferulic acid, the adenosine, and the chlorella extract is (1-5): (1-2);

wherein, three '1-5' can be independently 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.;

wherein, 1-2 can be 1, 1.2, 1.4, 1.6, 1.8, 2, etc.

Preferably, the preparation raw materials of the cream containing the anti-aging composition comprise 0.01-5% of the anti-aging composition, 0.01-10% of a skin conditioner, 1-5% of an emulsifier, 1-20% of a humectant and the balance of water by mass percentage based on 100% of the preparation raw materials of the cream containing the anti-aging composition.

The content of the anti-premature composition is 0.01 to 5%, and may be, for example, 0.01%, 0.02%, 0.05%, 0.1%, 0.15%, 0.2%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%, or the like.

The content of the skin conditioner is 0.01 to 10%, and may be, for example, 0.01%, 0.02%, 0.05%, 0.1%, 0.15%, 0.2%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or the like.

The content of the emulsifier is 1 to 5%, and may be, for example, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, or the like.

The content of the humectant may be 1 to 20%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, etc.

Preferably, the skin conditioner further comprises any one or a combination of at least two of a sophora flavescens root extract, a glycyrrhiza inflate root extract, a sunflower seed oil unsaponifiable matter, a rosemary leaf extract, niacinamide, a yeast/Sichuan millet seed fermentation product filtrate, a schizosaccharomyces cerevisiae fermentation product lysate, an animal umbilical cord extract, an animal placental protein or an animal placental enzyme.

Preferably, the skin conditioner comprises 0.1-0.5% of carnosine, 0.1-0.5% of ferulic acid, 0.1-0.5% of adenosine, 0.01-0.2% of chlorella extract, 0.01-0.1% of sophora flavescens root extract, 0.01-0.1% of glycyrrhiza inflate root extract, 0.01-0.1% of sunflower seed oil unsaponifiable matter, 0.01-0.1% of rosemary extract, 0.1-1% of nicotinamide, 0.01-0.1% of yeast/cuckoo seed fermentation product filtrate, 0.01-0.3% of schizosaccharomyces cerevisiae fermentation product lysate, 0.01-0.1% of animal umbilical cord extract, 0.01-0.1% of animal placental protein and 0.01-0.1% of animal placental enzyme, based on 100% of the mass of the raw materials for preparing the cream containing the anti-premature aging composition.

The content of carnosine may be, for example, 0.1 to 0.5%, for example, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%.

The content of ferulic acid is 0.1-0.5%, and may be, for example, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, etc.

The content of adenosine is 0.1-0.5%, and may be, for example, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, etc.

Wherein the content of Chlorella extract is 0.01-0.2%, such as 0.01%, 0.02%, 0.05%, 0.08%, 0.1%, 0.12%, 0.15%, 0.17%, 0.2%.

Wherein the content of radix Sophorae Flavescentis extract is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.

Wherein the content of the extract of Glycyrrhiza inflata root is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.

The content of the unsaponifiable sunflower seed oil is 0.01-0.1%, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.

The content of rosemary leaf extract is 0.01-0.1%, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.

The nicotinamide content is 0.1-1%, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc.

Wherein the content of yeast/Sichuan millet seed fermentation product filtrate is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.

The content of the secondary fission yeast fermentation product lysate may be, for example, 0.01 to 0.3%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%.

The content of the animal umbilical cord extract is 0.01-0.1%, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.

The content of animal placenta protein is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.

The content of animal placental enzyme is 0.01-0.1%, and may be, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.

Preferably, the emulsifier comprises any one or combination of at least two of sodium acrylate/sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80, C20-22 alcohol, C20-22 alcohol phosphate, polyacrylamide, C13-14 isoparaffin or laureth-7.

Preferably, the emulsifier includes 0.5-0.7% of sodium acrylate/sodium acryloyldimethyl taurate copolymer, 0.3-0.4% of isohexadecane, 0.01-0.02% of polysorbate-800.5-0.7% of C20-22 alcohol, 0.5-0.7% of C20-22 alcohol phosphate, 0.2-0.3% of polyacrylamide, 0.1-0.2% of C13-14 isoparaffin, and 0.25-0.04% of laureth-70.02, by mass of the preparation raw material of the cream containing the anti-aging composition.

The content of the sodium acrylate/sodium acryloyldimethyl taurate copolymer is 0.5 to 0.7%, and may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, or the like.

The content of isohexadecane is 0.3 to 0.4%, and may be, for example, 0.3%, 0.32%, 0.34%, 0.36%, 0.38%, 0.4%, or the like.

The content of polysorbate-80 is 0.01-0.02%, and may be, for example, 0.01%, 0.012%, 0.014%, 0.016%, 0.018%, 0.02%, etc.

The content of C20-22 alcohol is 0.5-0.7%, and may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, etc.

The content of C20-22 alcohol phosphate is 0.5-0.7%, and may be, for example, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, etc.

The content of polyacrylamide is 0.2 to 0.3%, and may be, for example, 0.2%, 0.22%, 0.24%, 0.26%, 0.28%, 0.3%, or the like.

The content of C13-14 isoparaffin is 0.1-0.2%, for example, 0.1, 0.12%, 0.14%, 0.16%, 0.18%, 0.2%.

The content of laureth-7 is 0.02-0.04%, for example, 0.02%, 0.025%, 0.03%, 0.035%, 0.04%, etc.

Preferably, the humectant comprises any one or a combination of at least two of butylene glycol, glyceryl polyether-26, trehalose, sodium hyaluronate, or 1, 2-hexanediol.

Preferably, the moisturizer comprises 5-10% of butanediol, 262-5% of glyceryl polyether, 2-4% of trehalose, 0.15-0.25% of sodium hyaluronate and 0.4-0.6% of 1, 2-hexanediol by mass of 100% of raw materials for preparing the cream containing the anti-aging composition.

The content of butanediol is 5 to 10%, and may be, for example, 5%, 6%, 7%, 8%, 9%, 10%, or the like.

The content of the glyceryl polyether-26 is 2 to 5%, and may be, for example, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, or the like.

The trehalose content is 2 to 4%, and may be, for example, 2%, 2.5%, 3%, 3.5%, 4%, or the like.

The content of sodium hyaluronate is 0.15-0.25%, for example, 0.15%, 0.17%, 0.2%, 0.23%, 0.25%, etc.

The content of 1, 2-hexanediol is 0.4 to 0.6%, and may be, for example, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, or the like.

Preferably, the raw materials for preparing the face cream also comprise any one or the combination of at least two of an emollient, a thickening agent, a pH regulator, a chelating agent, an antioxidant or a flavoring agent.

Preferably, the raw materials for preparing the facial cream containing the anti-aging composition also comprise 5-10% of an emollient, 0.5-1% of a thickening agent, 0.31-0.75% of a pH regulator, 0.01-0.05% of a chelating agent, 0.1-1% of an antioxidant and 0.01-0.05% of a flavoring agent, wherein the mass of the raw materials is 100%.

The content of the emollient is 5 to 10%, and may be, for example, 5%, 6%, 7%, 8%, 9%, 10%, etc.

The content of the thickener is 0.5 to 1%, and may be, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or the like.

The content of the pH adjuster is 0.31 to 0.75%, and may be, for example, 0.31%, 0.4%, 0.5%, 0.6%, 0.7%, 0.75%, or the like.

The content of the chelating agent is 0.01 to 0.05%, and may be, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05% or the like.

The content of the antioxidant is 0.1 to 1%, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, or the like.

The content of the aromatic may be 0.01 to 0.05%, for example, 0.01%, 0.015%, 0.02%, 0.025%, 0.03%, 0.04%, 0.05%, etc.

Preferably, the emollient comprises any one or a combination of at least two of squalane, isononyl isononanoate, or jojoba oil.

Preferably, the thickener comprises an ammonium acryloyldimethyltaurate/VP copolymer and/or a carbomer.

Preferably, the pH adjusting agent is arginine.

Preferably, the chelating agent is disodium EDTA.

Preferably, the antioxidant is p-hydroxyacetophenone.

Preferably, the fragrance is a perfume.

In a second aspect, the present invention provides a method of preparing a cream comprising an anti-aging composition according to the first aspect, the method comprising the steps of:

(1) mixing the humectant and water to obtain a water-phase mixed solution; mixing the emulsifier with an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) and (3) mixing and stirring the homogenized liquid obtained in the step (2), the anti-aging composition and the skin conditioner to obtain the cream containing the anti-aging composition.

Preferably, the temperature of the aqueous phase mixing in step (1) is 80-85 ℃, for example 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃ and the like, and the time of the aqueous phase mixing is 5-10min, for example 5min, 6min, 7min, 8min, 9min, 10min and the like.

Preferably, the temperature of the oil phase mixing in step (1) is 78-80 deg.C, such as 78 deg.C, 78.5 deg.C, 79 deg.C, 79.5 deg.C, 80 deg.C, etc., and the time of the oil phase mixing is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, in step (1), the aqueous phase is mixed with a thickener.

Preferably, in step (1), the oil phase is mixed with an emollient.

Preferably, the temperature for mixing and homogenizing in step (2) is 80-82 deg.C, such as 80 deg.C, 80.5 deg.C, 81 deg.C, 81.5 deg.C, 82 deg.C, etc., and the time for mixing and homogenizing is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, the temperature of the mixing and stirring in the step (3) is 40-50 ℃, for example, 40 ℃, 42 ℃, 44 ℃, 46 ℃, 48 ℃, 50 ℃ and the like, and the time of the mixing and stirring is 5-10min, for example, 5min, 6min, 7min, 8min, 9min, 10min and the like.

Preferably, in the step (3), any one or a combination of at least two of a pH regulator, a chelating agent, an antioxidant or a fragrance is further added to the mixing and stirring.

Compared with the prior art, the invention has the following beneficial effects:

(1) the components in the anti-aging composition and the skin conditioner are synergistic, so that the fragile and sensitive state of the skin caused by the external environment can be effectively reduced, the resistance of the skin is enhanced, the elasticity of the skin is improved, wrinkles are reduced, the skin is reddened, the moisture of the skin is increased, and the skin is kept in a healthy state of gloss and fineness;

(2) the active ingredients in the facial cream can effectively inhibit the generation of AGEs and reduce glycosylation reaction, and can obviously improve dark yellow skin, restore the previous luster of the skin and reduce the aging signs of the skin after long-term use.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Each of the components in the following preparation examples is commercially available.

Preparation example 1

The preparation example provides a curcuma alismatifolia root extract, which is prepared by the following preparation method:

(1) freezing root of Zingiber officinale Roscoe at-15 deg.C for 18h, pulverizing at-2 deg.C, and sieving to obtain 150 mesh Zingiber officinale Roscoe root powder;

(2) mixing the ginger flower root powder obtained in the step (1) with n-butanol, leaching for 2 hours at 25 ℃, and concentrating the solution obtained by leaching to obtain a concentrated solution; mixing the concentrated solution with ethanol, leaching at 40 deg.C for 1 hr, and filtering to obtain crude extract of rhizoma Zingiberis recens;

wherein the mass ratio of the ginger flower root powder to the n-butanol to the ethanol is 1:10: 8;

(3) and (3) passing the crude extract of the ginger flower roots obtained in the step (2) through macroporous adsorption resin AB-8, washing with deionized water, removing water solution, washing with 60 wt% ethanol water solution, collecting the washed ethanol water solution part, and concentrating to obtain the ginger flower root extract.

Preparation example 2

The preparation example provides a radix scutellariae extract, which is prepared by the following preparation method:

(a) pulverizing radix Scutellariae, and sieving to obtain 200 mesh radix Scutellariae powder;

(b) mixing the radix scutellariae powder obtained in the step (a) with an ethanol water solution with the concentration of 60 wt% according to the mass ratio of 1:10, performing microwave extraction for 8min at the power of 600W, and concentrating to obtain a radix scutellariae crude extract;

(c) dissolving the crude extract of radix scutellariae obtained in the step (b) by using 10 wt% of ethanol water solution, purifying by using a macroporous resin gel column D101, eluting by using 60 wt% of ethanol water solution, and concentrating to obtain the radix scutellariae extract.

Preparation example 3

The present preparation example provides a cocoa seed extract prepared by the following preparation method:

(A) pulverizing cacao seed to particle size of 100 mesh, mixing with n-hexane at a mass ratio of 1:10, soaking at 30 deg.C for 3 hr, filtering, and drying to obtain defatted cacao seed powder;

(B) mixing the defatted cocoa seed powder obtained in the step (A) with water, heating to 45 ℃, mixing with biological enzyme and acid, wherein the mass ratio of the defatted cocoa seed powder to the water to the cellulase is 1:15:0.006, carrying out enzymolysis reaction for 1.5h at 45 ℃ and pH of 5, and then inactivating to obtain a cocoa seed enzymolysis product;

(C) mixing the cocoa seed enzymolysis product obtained in the step (B) with 60 wt% ethanol water solution according to the mass ratio of 1:10, performing ultrasonic extraction for 50min at the power of 300W, filtering, and concentrating to obtain the crude extract of cocoa seeds.

Except that the ginger flower root extract, the scutellaria root extract and the cacao seed extract were prepared by the above-mentioned preparation methods, the remaining components in the following examples and comparative examples were all commercially available.

Example 1

This example provides an anti-aging composition comprising 6 parts of a ginger flower root extract, 10 parts of a scutellaria root extract and 4 parts of a cocoa seed extract.

Example 2

This example provides an anti-aging composition comprising, in parts by weight, 5 parts of a ginger flower root extract, 9 parts of a scutellaria root extract, and 6 parts of a cocoa seed extract.

Example 3

This example provides an anti-aging composition comprising, in parts by weight, 7 parts of a ginger flower root extract, 8 parts of a scutellaria root extract, and 5 parts of a cocoa seed extract.

Example 4

This example provides an anti-aging composition comprising, in parts by weight, 3 parts of a ginger flower root extract, 15 parts of a scutellaria root extract, and 2 parts of a cocoa seed extract.

Example 5

This example provides an anti-aging composition comprising, in parts by weight, 8 parts of a ginger flower root extract, 5 parts of a scutellaria root extract, and 7 parts of a cocoa seed extract.

Comparative example 1

The present comparative example provides a composition comprising, in parts by weight, 8 parts of a zingiber officinale root extract, 12 parts of a scutellaria baicalensis root extract.

Comparative example 2

This comparative example provides a composition comprising, by weight, 11 parts of a ginger flower root extract, 9 parts of a cocoa seed extract.

Comparative example 3

The present comparative example provides a composition comprising, in parts by weight, 8 parts of scutellaria root extract, 12 parts of cocoa seed extract.

Application example 1

The application example provides a facial cream containing an anti-aging composition, and the preparation raw materials of the facial cream containing the anti-aging composition comprise the following components in percentage by mass:

the preparation method of the cream containing the anti-aging composition comprises the following steps:

(1) stirring and mixing the humectant, the thickener and water at 82 ℃ for 8min to obtain a water phase mixed solution; stirring and mixing the emollient and the emulsifier for 8min at 80 ℃ to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) at 80 ℃ and homogenizing for 8min to obtain homogenized liquor;

(3) and (3) mixing and stirring the homogenized liquid obtained in the step (2), the anti-aging composition, the skin conditioner, the pH regulator, the chelating agent, the antioxidant and the aromatic at 45 ℃ for 6min to obtain the cream.

Application example 2

the preparation method of the cream containing the anti-aging composition in the application example is the same as that in application example 1.

Application example 3

Application example 4

Application example 5

The present application example provides a cream, which is different from application example 1 in that the anti-aging composition provided in example 1 is replaced with the anti-aging composition provided in example 4 of equal quality, and the contents of other components and the preparation method are the same as those of application example 1.

Application example 6

The present application example provides a cream, which is different from application example 1 in that the anti-aging composition provided in example 1 is replaced with the anti-aging composition provided in example 5 of equal mass, and the contents of other components and the preparation method are the same as in application example 1.

Application example 7

The application example provides a facial cream, which is different from the application example 1 in that the extract of the root of the sophora flavescens ait is not added, the content of the extract of the root of the glycyrrhiza inflata is increased to 0.1%, and the content of other components and the preparation method are the same as the application example 1.

Application example 8

The application example provides a facial cream, which is different from the application example 1 in that no glycyrrhiza inflate extract is added, the content of the sophora flavescens root extract is increased to 0.1%, and the content of other components and the preparation method are the same as the application example 1.

Application example 9

The application example provides a cream, and is different from the application example 1 in that rosemary leaf extract is not added, the content of unsaponifiable matter in sunflower seed oil is increased to 0.1%, and the content of other components and the preparation method are the same as the application example 1.

Application example 10

The application example provides a cream, and is different from the application example 1 in that sunflower seed oil unsaponifiable matter is not added, the content of rosemary leaf extract is increased to 0.1%, and the content of other components and the preparation method are the same as the application example 1.

Application example 11

The application example provides a cream, and is different from the application example 1 in that the lysate of the yeast schizosaccharomyces cerevisiae fermentation product is not added, the content of the filtrate of the yeast/fructus kaki siccus fermentation product is increased to 0.4%, and the content of other components and the preparation method are the same as the application example 1.

Application example 12

The application example provides a cream, and is different from the application example 1 in that an animal umbilical cord extract, animal placental protein and animal placental enzyme are not added, the content of a lysate of a fermentation product of the saccharomyces bifidus is increased to 0.5%, and the content of other components and a preparation method are the same as those of the application example 1.

Comparative application example 1

This comparative application example provides a cream, which is different from application example 1 in that the anti-premature composition provided in example 1 was replaced with the composition provided in comparative example 1 of equal quality, and the contents of other components and the preparation method were the same as in application example 1.

Comparative application example 2

This comparative application example provides a cream, which is different from application example 1 in that the anti-premature composition provided in example 1 was replaced with the composition provided in comparative example 2 of equal quality, and the contents of other components and the preparation method were the same as in application example 1.

Comparative application example 3

This comparative application example provides a cream, which is different from application example 1 in that the anti-premature composition provided in example 1 was replaced with the composition provided in comparative example 3 of equal quality, and the contents of other components and the preparation method were the same as in application example 1.

Comparative application example 4

The comparison application example provides a cream, and is different from the application example 1 in that carnosine is not added, the content of ferulic acid is increased to 0.3%, the content of adenosine is increased to 0.4%, the content of chlorella extract is increased to 0.3%, and the content of other components and the preparation method are the same as the application example 1.

Comparative application example 5

The comparison application example provides a cream, and is different from the application example 1 in that no ferulic acid is added, the carnosine content is increased to 0.5%, the adenosine content is increased to 0.35%, the chlorella extract content is increased to 0.15%, and the content of other components and the preparation method are the same as the application example 1.

Comparative application example 6

The comparison application example provides a cream, and is different from the application example 1 in that adenosine is not added, the carnosine content is increased to 0.5%, the ferulic acid content is increased to 0.3%, the chlorella extract content is increased to 0.2%, and the content of other components and the preparation method are the same as the application example 1.

Comparative application example 7

The comparison application example provides a face cream, which is different from the application example 1 in that adenosine is not added, the carnosine content is increased to 0.44%, the ferulic acid content is increased to 0.24%, the adenosine content is increased to 0.32%, and the content of other components and the preparation method are the same as the application example 1.

Test example 1

Safety performance testing

Safety performance tests were performed on the creams provided in application examples 1-12 and the creams provided in comparative application examples 1-7, as follows:

(1) haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Select samples were diluted with PBS solution to different concentrations, with 5 concentration gradients set for each sample. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing its absorbance at 560nm with spectrophotometer (A)₅₆₀) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated₅₀)。

(2) Protein denaturation experiments:

diluting the sample to 10g/L with PBS solution, collecting 10mL dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing absorbance A at 540nm and 575nm with spectrophotometer₅₄₀And A₅₇₅Calculating a protein denaturation index (D) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀The following table 1 shows the experimental irritation grading criteria for erythrocyte hemolysis:

TABLE 1

L/D	Grading
		>100	Has no irritation
10<L/D≤100	Micro-stimulation property
		1<L/D≤10	Mild irritation
0.1<L/D≤1	Moderate irritation

The results of the above-described hemolysis test and protein denaturation test are shown in Table 2 below:

TABLE 2

As can be seen from the safety performance test, the face cream prepared by the application examples 1-12 is mild and non-irritant according to the test results; the sample concentration HD of the cream prepared by the invention when 50% red blood cells are hemolyzed₅₀HD more than 14000mg/L than that of the face cream prepared in the comparative application examples 1 to 7₅₀(ii) a Meanwhile, the protein denaturation index DI is below 2.55 percent and is also obviously smaller than the face cream prepared by comparative application examples 1-7, which shows that the addition of the anti-aging composition and the specific skin conditioner can obviously reduce the toxic and side effects and irritation of the face cream, and is safer and more reliable.

Test example 2

Anti-inflammatory assay

Anti-inflammatory tests were performed on the creams provided in application examples 1-12 and on the creams provided in comparative application examples 1-7 as follows:

(1) cell collection: mixing blood sample with blood cell separating medium at equal ratio, centrifuging at 20 deg.C for 40min, collecting human peripheral blood mononuclear cell layer, washing cells with buffer solutionSuspending the cells in a medium RPMI-1640; CD14+ monocytes in human peripheral blood mononuclear cells were then purified with CD14+ microspheres (MiniMACS system); the differentiation of cells into dendritic cells is stimulated by the cytokine IL-4 and the growth hormone GM-CSF at 37 ℃ with 5% CO₂Culturing for 6 days, collecting differentiated dendritic cells and inoculating the dendritic cells into a pore plate;

(2) each composition was added to a well plate at a concentration of 2. mu.g/mL, and wells to which an equal volume of medium was added were set as blank controls, 37 ℃ and 5% CO₂Co-culturing for 48 hours; the cell culture supernatant was collected. Detecting the content of interleukin IL-10 and IL-12 in the supernatant by using an enzyme-linked immunosorbent assay (ELISA), wherein the content of the anti-inflammatory cytokine IL-10 in a blank control group is defined as 0, and the content of the proinflammatory cytokine IL-12 in the blank control group is defined as 100%;

the specific test results are shown in table 3:

TABLE 3

As can be seen from the data in Table 3, the cream interleukin provided for the application examples 1-12 of the present invention in the culture medium has IL-10 content of more than 150% and interleukin IL-12 level of less than 15%, which fully indicates that the anti-aging composition of the present invention can significantly increase the level of anti-inflammatory cytokine interleukin IL-10 and inhibit the expression level of proinflammatory cytokine interleukin IL-12; the anti-aging composition can be matched with each component in a skin conditioner of cream to realize synergistic interaction by regulating the redox balance of cells and regulating autophagy of cells, so that the anti-inflammatory capability is further improved.

Test example 3

Anti-glycation and anti-photoaging assays

(1) Anti-glycation test according to fluorescence of AGEs: the inhibition of AGEs formation by each group of compositions was evaluated using a glucose- β -Lg mimetic system. According to the fluorescence of AGEs, an F-4600 fluorescence spectrometer is adopted to measure the fluorescence intensity of fluorescent AGEs after a blank control group and a composition test group are added for reaction for 7d under the conditions of an excitation wavelength of 325nm and an emission wavelength of 440nm, and the inhibition rate of fluorescent AGEs is calculated (wherein each group of test samples is diluted to 5.0 wt% by adopting a phosphate buffer solution), and the calculation method is as follows:

inhibition (%) [ (F0-F1)/F0] × 100%;

wherein, F0-fluorescence intensity of blank control group; f1 — fluorescence intensity of test group; (ii) a

(2) The oxidative stress test of cuticle keratinocyte under blue light comprises the following specific test methods: oxidizing organotypic skin model with blue light radiation, pre-treating with a test sample anti-photoaging composition and then irradiating with blue light at a blue light intensity of 15J/cm². Since moderate to high doses of ROS (reactive oxygen species) induce apoptosis and even cause necrosis of cells through oxidative stress of the cells, measuring the accumulation of ROS in epidermal keratinocytes can reflect the photoaging resistance of the test samples.

The specific test results are shown in table 4:

TABLE 4

As shown in the test data in Table 4, the inhibition rate of the cream prepared in application examples 1-12 of the invention on fluorescent AGEs is more than 92%, and the accumulation amount of ROS epidermal keratinocytes is less than 10%, so that the cream can obviously improve photoaging and help cell saccharification reversion compared with the cream prepared in application comparative examples 1-7. The anti-aging composition can be matched with each component in the skin conditioner of the cream, so that the synergistic effect is achieved, the cell saccharification reversion is facilitated, the glycosylation reaction is reduced, the dark yellow skin can be obviously improved after long-term use, the previous luster of the skin is recovered, the skin aging signs are reduced, and the active oxygen is reduced under the blue light pressure, so that the blue light effect is achieved.

Test example 4

Experiment for promoting elastin production

An experiment for promoting elastin production was performed on the creams provided in application examples 1-12 and the creams provided in comparative application examples 1-7, as follows:

(1) inoculating human fibroblasts on a 96-well plate according to the density of 20000 cells per well, adding a culture medium, culturing for 24 hours in an incubator at 37 ℃, replacing the culture medium after the cells adhere to the wall, adding each test sample (controlling the final concentration of the sample to be 50mg/mL), taking a PBS solution of 20mmol/L as a blank control, and then culturing for 2 days in the incubator at 37 ℃.

(2) After the culture, the cells were eluted with a cell lysis solution and disrupted by sonication. Detecting the content of the elastin by adopting a Biocolor elastin detection kit: treating a sample with an elastin precipitator to precipitate elastin, performing centrifugal separation, removing supernatant, adding a dye and a reaction solution into the precipitate to react to form an elastin-dye complex, performing centrifugal separation and washing with a PBS solution for three times to remove unbound dye, adding a dye releasing agent to release the dye in the elastin-dye complex, measuring the absorbance of the sample at 513nm by using a spectrophotometer, and calculating the expression quantity of elastin according to the absorbance-dye concentration standard curve (the expression quantity of a blank group is 1);

the specific test results are shown in table 5 below:

TABLE 5

As can be seen from the test data in table 5, the cream provided in application examples 1 to 12 of the present invention has an elastin expression level of 4.5 or more, which fully indicates that the anti-aging composition can cooperate with each component of the skin conditioner of the cream to synergistically enhance the elastin expression, stimulate cell growth, increase skin elasticity, and thus lighten and shorten wrinkles, and improve skin conditions.

Test example 5

Anti-wrinkle effect experiment

Carrying out an anti-wrinkle effect experiment on the face creams provided in the application examples 1-12 and the face creams provided in the comparative application examples 1-7; the specific test method is as follows:

(1) subject: 190 female volunteers with healthy skin and ages of 35-55 are selected, the proportion of the male and female volunteers is half respectively, and the male and female volunteers are randomly divided into 19 groups;

(2) an experimental instrument: skin wrinkle tester VL650 from CK, germany;

(3) the test method comprises the following steps: adopting a skin wrinkle tester VL650 of Germany CK company, sleeving the forehead with silica gel to measure the wrinkle depth, respectively testing the forehead wrinkles of a tested person, and taking the average value of three times of test data; the specific application method of the face cream is that 8 spots in the morning are used for cleaning faces, and 0.4g of each test sample face cream is used on the face after the faces are cleaned; cleaning face at 8 o' clock at night, and respectively using 0.4g of each test sample cream on the face after cleaning face, wherein the duration of the experiment period is 60 days; finally calculating the percentage of wrinkle depth reduction after 30 and 60 days of treatment;

the specific test results are shown in table 6 below:

TABLE 6

As can be seen from the test data in Table 6, the percentage of wrinkle depth reduction achieved after 60 days in the creams provided by application examples 1-12 of the present invention is tested to be 19% or more, which fully demonstrates that the anti-aging composition is effective in retarding the aging process of the skin by counteracting skin wrinkles, reducing the generation of static lines, and improving the dynamic lines of the skin by promoting the expression of skin elastin and the proliferative growth of cells, by cooperating with the ingredients in the skin conditioner of the cream, synergistically.

The applicant states that the invention is illustrated by the above examples of a cream containing an anti-aging composition and a method of making the same, but the invention is not limited to the above examples, i.e. it is not meant that the invention must be practiced by relying on the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A facial cream containing an anti-aging composition is characterized in that the facial cream containing the anti-aging composition is prepared from the raw materials of the anti-aging composition, a skin conditioner, an emulsifier, a humectant and water;

2. The cream containing an anti-aging composition as claimed in claim 1, wherein the anti-aging composition is characterized in that the mass ratio of the ginger flower root extract, the scutellaria root extract and the cacao seed extract is (3-8): (5-15): (2-7);

preferably, in the skin conditioner, the mass ratio of the carnosine, the ferulic acid, the adenosine, and the chlorella extract is (1-5): (1-2).

3. The cream containing the anti-aging composition according to claim 1 or 2, characterized in that the raw materials for preparing the cream containing the anti-aging composition comprise, by mass percent, 0.01-5% of the anti-aging composition, 0.01-10% of a skin conditioner, 1-5% of an emulsifier, 1-20% of a humectant and the balance of water, based on 100% of the raw materials for preparing the cream containing the anti-aging composition.

4. The cream containing an anti-aging composition according to any one of claims 1 to 3, wherein the skin conditioner further comprises any one or a combination of at least two of Sophora flavescens root extract, Glycyrrhiza inflate root extract, sunflower seed oil unsaponifiable matter, rosemary leaf extract, niacinamide, yeast/Sichuan millet seed fermentation product filtrate, yeast bifida fermentation product lysate, animal umbilical cord extract, animal placental protein, or animal placental enzyme;

preferably, the skin conditioner comprises 0.1-0.5% of carnosine, 0.1-0.5% of ferulic acid, 0.1-0.5% of adenosine, 0.01-0.2% of chlorella extract, 0.01-0.1% of sophora flavescens root extract, 0.01-0.1% of glycyrrhiza inflata root extract, 0.01-0.1% of sunflower seed oil unsaponifiable matter, 0.01-0.1% of rosemary leaf extract, 0.1-1% of nicotinamide, 0.01-0.1% of yeast/ligusticum wallichii fermentation product filtrate, 0.01-0.3% of schizosaccharomyces cerevisiae fermentation product lysate, 0.01-0.1% of animal umbilical cord extract, 0.01-0.1% of animal placental protein and 0.01-0.1% of animal placental enzyme, based on 100% of the mass of the raw materials for preparing the cream containing the anti-premature aging composition.

5. The cream containing an anti-aging composition according to any one of claims 1 to 4, wherein the emulsifier comprises any one of sodium acrylate/sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80, C20-22 alcohol, C20-22 alcohol phosphate, polyacrylamide, C13-14 isoparaffin, or laureth-7, or a combination of at least two thereof;

6. A cream containing an anti-aging composition according to any of claims 1 to 5, characterized in that the moisturizer comprises any one or a combination of at least two of butylene glycol, glyceryl polyether-26, trehalose, sodium hyaluronate or 1, 2-hexanediol;

7. The cream containing an anti-aging composition according to any one of claims 1 to 6, wherein the cream is prepared by further comprising any one or a combination of at least two of an emollient, a thickener, a pH adjuster, a chelating agent, an antioxidant, or a fragrance;

preferably, the raw materials for preparing the facial cream containing the anti-aging composition also comprise 5-10% of an emollient, 0.5-1% of a thickening agent, 0.31-0.75% of a pH regulator, 0.01-0.05% of a chelating agent, 0.1-1% of an antioxidant and 0.01-0.05% of a flavoring agent, wherein the mass of the raw materials is 100%;

preferably, the emollient comprises any one or combination of at least two of squalane, isononyl isononanoate, or jojoba oil;

preferably, the thickener comprises an ammonium acryloyldimethyltaurate/VP copolymer and/or a carbomer;

preferably, the pH adjusting agent is arginine;

preferably, the chelating agent is disodium EDTA;

preferably, the antioxidant is p-hydroxyacetophenone;

preferably, the fragrance is a perfume.

8. A method of preparing a cream containing an anti-aging composition according to any of claims 1 to 7, wherein the cream is prepared by a method comprising the steps of:

9. The method for preparing a cream containing an anti-aging composition according to claim 8, wherein in the step (1), the temperature of the aqueous phase mixing is 80 to 85 ℃ and the time of the aqueous phase mixing is 5 to 10 min;

preferably, in the step (1), the temperature of the oil phase mixing is 78-80 ℃, and the time of the oil phase mixing is 5-10 min;

preferably, in step (1), the aqueous phase is mixed with a thickening agent;

preferably, in the step (1), an emollient is also added to the oil phase mixture;

preferably, in the step (2), the temperature for mixing and homogenizing is 80-82 ℃, and the time for mixing and homogenizing is 5-10 min.

10. The method for preparing a cream containing an anti-aging composition according to claim 8 or 9, wherein in the step (3), the temperature of the mixing and stirring is 40 to 50 ℃, and the time of the mixing and stirring is 5 to 10 min;