CN112641702A

CN112641702A - Wrinkle-removing and anti-aging composition and preparation method and application thereof

Info

Publication number: CN112641702A
Application number: CN202011576709.8A
Authority: CN
Inventors: 孟铁钢
Original assignee: Guangzhou Q Max Cosmetics Co ltd
Current assignee: Guangzhou Q Max Cosmetics Co ltd
Priority date: 2020-12-28
Filing date: 2020-12-28
Publication date: 2021-04-13

Abstract

The invention relates to the field of cosmetics, in particular to a wrinkle-removing and anti-aging composition, and further discloses an application of the wrinkle-removing and anti-aging composition in preparation of a wrinkle-removing and anti-aging product. The wrinkle-removing and anti-aging composition comprises plant source anti-aging extracts, palmitoyl pentapeptide-4, protein peptide, rosemary extracts and glycogen which are used as effective components, is mild and non-irritant in raw material components, can be used as wrinkle-removing and anti-aging active components, is applied to various cosmetics, and has ideal wrinkle-removing, moisturizing and anti-aging effects.

Description

Wrinkle-removing and anti-aging composition and preparation method and application thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a wrinkle-removing and anti-aging composition, and further discloses an application of the wrinkle-removing and anti-aging composition in preparation of a wrinkle-removing and anti-aging product.

Background

With the rapid development of economy and the improvement of living standard of people, anti-aging cosmetics are more and more valued, and meanwhile, the consumption concept of consumers tends to be rational, and the requirements on the care efficacy and safety of the cosmetics are gradually improved. Many cosmetic enterprises and research institutions invest a lot of manpower and material resources to research and develop novel products, and cosmetics added with new anti-aging ingredients are increasing.

Human skin is an organ that readily appears to be aged, usually as indicated by the development of wrinkles. Skin aging is largely classified into two types, one is intrinsic aging and the other is extrinsic aging. Intrinsic aging is mainly accompanied by the aging characteristics of the skin, especially with increasing age: increased wrinkles, loose, yellow, shiny, glossy and smooth skin, coarser texture, increased mottle, etc.; exogenous aging mainly means that the damage of skin cells is accelerated and the natural aging speed is increased due to night-out, working pressure, life anxiety, ultraviolet injury and the like. In addition, there is an expression line generated by the personal expression habit and cannot be avoided by self-consciousness, and the skin is repeatedly stretched and relaxed by muscles, so that collagen is rearranged and wrinkles are finally formed with the lapse of time.

Based on the aim of pursuing natural green, safe and healthy, natural plant cosmetics are generally popular with consumers due to the characteristics of nature, mildness, no stimulation, high safety and the like. The product is prepared by extracting the effective components of the plants and combining the formula of the cosmetic matrix, and becomes the mainstream of the international natural plant cosmetic product research and development.

Disclosure of Invention

The invention provides a wrinkle-removing and anti-aging composition which has better moisturizing, anti-oxidation, wrinkle-removing and anti-aging effects;

the second technical problem to be solved by the invention is to provide a wrinkle-removing and anti-aging cosmetic which can meet the daily requirements of wrinkle removal and anti-aging.

In order to achieve the purpose, the invention provides a wrinkle-removing and anti-aging composition which is characterized by comprising the following components in percentage by mass:

specifically, the wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 0.0001-20 wt% of botanical anti-aging extract, 0.0001-20 wt% of palmitoyl pentapeptide-40.0001-20 wt%, 0.0001-20 wt% of protein peptide, 0.0001-15 wt% of rosemary extract, 0.0001-15 wt% of glycogen and the balance of water.

Specifically, the wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 0.0001-20 wt% of botanical anti-aging extract, 0.0001-20 wt% of palmitoyl pentapeptide-40.0001-20 wt%, 0.0001-10 wt% of protein peptide, 0.0001-15 wt% of rosemary extract, 0.0001-15 wt% of glycogen and the balance of water.

In another preferred embodiment, the wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 1-10 wt% of botanical anti-aging extract, 41-10 wt% of palmitoyl pentapeptide, 1-10 wt% of protein peptide, 1-5 wt% of rosemary extract, 1-5 wt% of glycogen and the balance of water.

In another preferred embodiment, the wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 1-15 wt% of botanical anti-aging extract, 41-15 wt% of palmitoyl pentapeptide, 1-10 wt% of protein peptide, 1-10 wt% of rosemary extract, 1-10 wt% of glycogen and the balance of water.

In another preferred embodiment, the wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 1-15 wt% of botanical anti-aging extract, 41-15 wt% of palmitoyl pentapeptide, 1-6 wt% of protein peptide, 1-10 wt% of rosemary extract, 1-10 wt% of glycogen and the balance of water.

Specifically, the plant-derived anti-aging extract comprises an aconite leaf cowpea seed extract, a calanthe extract and/or an India swertia extract. The plant source anti-aging extract is a plant source retinol-like extract, and the selected cowpea seed extract, the calanthe extract and the swertia indica extract have anti-aging mechanism and anti-aging effect similar to retinol, can promote the synthesis of various growth factors by improving the expression of cell information and stimulating cells to metabolize, and the factors stimulate fibroblasts to synthesize protein so as to regenerate and proliferate the fibroblasts, and also have the anti-wrinkle effect.

Specifically, palmitoyl pentapeptide-4 selected in the composition is a bioactive peptide, can simulate a messenger peptide, which is a hydrolysate of a procollagen alpha 1 chain, and promotes the synthesis of collagen, so that the skin thickness is increased, wrinkles are reduced, and the composition has the effects of improving wrinkles and increasing skin elasticity.

Specifically, the protein peptide comprises arginine/lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and/or acetyl hexapeptide-8, and nerve conduction signals can be weakened by reducing the release of acetylcholine, so that muscle relaxation is realized, and expression stripes are efficiently controlled. Preferably, the arginine/lysine polypeptide preferably has a molecular weight of 2300-.

Specifically, the rosemary extract selected by the composition is extracted by supercritical carbon dioxide, oil is used as a cosolvent, and the extraction is carried out at a lower extraction temperature, wherein the rosemary extract mainly comprises diterpene phenolic compounds, flavonoids and triterpenes, and has the effects of resisting oxidation, improving skin elasticity and delaying aging.

Specifically, the glycogen is a product obtained by taking non-transgenic waxy corn starch as a raw material through an enzymatic reaction, such as an enzymatic hydrolysis reaction of enzyme preparations such as branching enzyme, amylomaltase, short-chain amylase, isoamylase and the like, and the enzymatic hydrolysis glycogen can improve the generation amount of ATP and the generation of cell self hyaluronic acid and ceramide, has the effects of moisturizing, resisting and delaying endogenous aging, resisting UVB oxidative damage, inhibiting ultraviolet irradiation induced apoptosis and relieving exogenous aging caused by ultraviolet and UVB oxidative damage.

The invention also discloses a method for preparing the wrinkle-removing and anti-aging composition, which comprises the step of adding selected amounts of the botanical anti-aging extract, the palmitoyl pentapeptide-4, the protein peptide, the rosemary extract and the glycogen into water and fully mixing the materials uniformly.

The invention also discloses application of the wrinkle-removing and anti-aging composition in preparing cosmetics with wrinkle-removing and anti-aging effects.

The invention also discloses a wrinkle-removing and anti-aging cosmetic which comprises the wrinkle-removing and anti-aging composition and acceptable auxiliary materials.

Specifically, in the wrinkle-removing and anti-aging cosmetic, the wrinkle-removing and anti-aging composition is 1-20 wt%, preferably 1-15 wt%, and more preferably 12 wt%.

Specifically, the wrinkle-removing and anti-aging cosmetics comprise wrinkle-removing and anti-aging essence, wrinkle-removing and anti-aging eye cream, wrinkle-removing and anti-aging mask paste, wrinkle-removing and anti-aging emulsion or wrinkle-removing and anti-aging face cream.

The invention also discloses a method for preparing the wrinkle-removing and anti-aging cosmetic, which comprises the steps of preparing the required wrinkle-removing and anti-aging composition according to the method, adding acceptable auxiliary materials, and preparing the cosmetics of the selected category according to the conventional process.

The wrinkle-removing and anti-aging composition comprises an anti-aging extract of an opsono-source, palmitoyl pentapeptide-4, protein peptide, rosemary extract and glycogen which are used as effective components; the plant-derived anti-aging extract is a natural plant-derived component extracted from plants, has an anti-aging mechanism and anti-aging effect similar to retinol, and has a good anti-wrinkle effect by improving cell information expression, stimulating cells to metabolize and promoting synthesis of growth factors and collagen; palmitoyl pentapeptide-4 has effects of improving wrinkle and increasing skin elasticity; the protein peptide weakens nerve conduction signals by reducing the release of acetylcholine, so that muscle relaxation is realized, and expression stripes are efficiently controlled; the rosemary extract has the effects of delaying aging and resisting oxidation; the sugar principle has the effects of moisturizing, resisting aging and resisting UVB oxidative damage. The raw material components of the wrinkle-removing and anti-aging composition are mild and non-irritant, can be used as wrinkle-removing and anti-aging active components, is applied to various cosmetics, and has ideal wrinkle-removing, moisturizing and anti-aging effects.

Drawings

FIG. 1 shows the results of reducing facial wrinkles in subjects with different compositions in the experimental examples of the present invention;

FIG. 2 shows the comparison results of the fine lines of the eyes of the test subjects in examples 7 and 8; wherein, the left image is eye cream of a left eye smearing example 8, and the right image is eye cream of a right eye smearing example 7;

FIG. 3 shows the results of comparing the fine lines of the eyes of the test subjects in examples 9 and 10; wherein, the left picture is the cream of the left face smearing example 10, and the right picture is the cream of the right face smearing example 9;

FIG. 4 is a comparison of the moisture content of the skin of the test subjects of examples 5 and 6; wherein, the comparison chart of skin moisture content of the A group and the B group is obtained;

fig. 5 is an antioxidant test using rosemary extract, on the left side DPPH absolute ethanol solution with rosemary extract added and on the right side control.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

In the following preparation examples and embodiment schemes of the invention, the selected raw material components are characterized by comprising the following components:

aconite leaf cowpea seed extract: a protein extract is extracted from Aconitum carmichaeli seed, wherein the seed contains carbohydrate (56-62%), protein (22-24%), water (11%), lipid (19%), and polyphenols (1.3%);

calanthe extract: adopting a culture process of plant stem cells, inducing the cells in vitro on a calanthe mitrella plant to perform a dedifferentiation process to generate callus, selecting a callus block with the most vitality, and culturing under different media and conditions so as to optimize the growth rate of solid-phase cells;

extractive of India swertia: pure roburin extracted from indian gentian leaf and containing 1% -2% of pure roburin;

arginine/lysine polypeptide: the molecular weight is 2300-;

dipeptide diaminobutyrylbenzylamide diacetate: molecular weight is 450-550;

acetyl hexapeptide-8: molecular weight is 800-;

rosemary extract: the extraction process of the selected rosemary extract adopts supercritical carbon dioxide extraction, takes oil as a cosolvent and is carried out at a lower extraction temperature, and the rosemary extract mainly comprises diterpene phenolic compounds, flavonoids and triterpenes;

glycogen: the glycogen is a commercially available product, non-transgenic waxy corn starch maltodextrin is selected as a raw material, the raw material is sequentially subjected to isoamylase enzymolysis and branching enzyme and amylase combined enzymolysis, the obtained enzymolysis product is subjected to enzyme deactivation, oligosaccharide is removed by filtration, the obtained enzymolysis product is further subjected to deionization treatment, and the obtained product is subjected to heating sterilization and spray drying, is sieved and is detected by a metal detector.

Preparation example 1

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 3% of an aconite leaf cowpea seed extract, 4% of palmitoyl pentapeptide-4, 3% of arginine/lysine polypeptide, 3% of a rosemary extract, 0.5% of glycogen and the balance of water.

At normal temperature, the components with the selected formula amount are added into water, stirred and dissolved uniformly, and the wrinkle-removing and anti-aging composition with the wrinkle-removing and anti-aging skin efficacy is obtained.

Preparation example 2

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 3% of an aconite leaf cowpea seed extract, 4% of palmitoyl pentapeptide-4, 3% of arginine/lysine polypeptide, 3% of a rosemary extract, 1% of glycogen and the balance of water.

Preparation example 3

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 6% of an aconite leaf cowpea seed extract, 4% of palmitoyl pentapeptide-4, 3% of arginine/lysine polypeptide, 3% of a rosemary extract, 0.5% of glycogen and the balance of water.

Preparation example 4

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 3% of an aconite leaf cowpea seed extract, 4% of palmitoyl pentapeptide-4, 4% of arginine/lysine polypeptide, 3% of a rosemary extract, 0.5% of glycogen and the balance of water.

Preparation example 5

The wrinkle-removing and anti-aging composition comprises the following components in mass content d: 0.0001% of an extract of cowpea seed of Aconitum carmichaeli Debx, 20% of palmitoyl pentapeptide-4, 0.0001% of arginine/lysine polypeptide, 20% of an extract of Rosmarinus officinalis, 0.0001% of glycogen, and the balance of water.

Preparation example 6

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 20% of calanthe extract, 0.0001% of palmitoyl pentapeptide-4, 20% of dipeptide diaminobutyrylbenzylamide diacetate, 0.0001% of rosemary extract, 20% of glycogen and the balance of water.

Preparation example 7

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 10% of India swertia extract, 10% of palmitoyl pentapeptide-4, 10% of arginine/lysine polypeptide, 15% of rosemary extract, 15% of glycogen and the balance of water.

Preparation example 8

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 10% of calanthe extract, 10% of palmitoyl pentapeptide-4, 10% of acetyl hexapeptide-8, 5% of rosemary extract, 5% of glycogen and the balance of water.

Preparation example 9

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 15% of India swertia extract, 15% of palmitoyl pentapeptide-4, 10% of arginine/lysine polypeptide, 10% of rosemary extract, 10% of glycogen and the balance of water.

Preparation example 10

The wrinkle-removing and anti-aging composition comprises the following components in percentage by mass: 15% of calanthe extract, 15% of palmitoyl pentapeptide-4, 6% of dipeptide diaminobutyrylbenzylamide diacetate, 10% of rosemary extract, 10% of glycogen and the balance of water.

Example 1 preparation and application of wrinkle-removing and anti-aging essence

The wrinkle-removing and anti-aging essence comprises the following raw materials:

phase A: water (To 100%), xanthan gum 0.5%, butanediol 4%, disodium EDTA 0.01%;

phase B: 0.5 percent of p-hydroxyacetophenone;

and C phase: preparation example 1 of the present invention wrinkle-removing and anti-aging composition 10%;

phase D: 0.5 percent of 1, 2-hexanediol.

The preparation process of the wrinkle-removing and anti-aging essence comprises the following steps:

(1) adding phase A into an emulsifying pot, homogenizing for 3-5 min, heating to 85 deg.C, stirring to dissolve completely, adding phase B, stirring, and cooling;

(2) cooling to below 45 ℃, adding C, D phases, stirring uniformly, defoaming, reporting and checking to be qualified, and filtering and discharging by using 150-mesh filter cloth to obtain the wrinkle-removing and anti-aging essence.

Example 2 preparation and application of wrinkle-removing and anti-aging essence

phase A: water (To 100%), xanthan gum 0.5%, butanediol 4%, disodium EDTA 0.01%;

phase B: 0.5 percent of p-hydroxyacetophenone;

and C phase: preparation example 2 of the present invention wrinkle-removing and anti-aging composition 10%;

phase D: 0.5 percent of 1, 2-hexanediol.

(1) adding phase A into emulsifying pot, homogenizing for 3-5 min, heating to 85 deg.C, stirring to dissolve completely, adding phase B, stirring, and cooling.

(2) Cooling to below 45 deg.C, adding C, D phase, stirring, defoaming, inspecting, and filtering with 150 mesh filter cloth to obtain the final product.

EXAMPLE 3 preparation and application of wrinkle-removing anti-aging eye cream

The raw materials of the wrinkle-removing and anti-aging eye cream comprise:

phase A: water (To 100%), ammonium acryloyldimethyltaurate/VP copolymer 0.6%, butanediol 2%, glycerol 6%;

phase B: glyceryl stearate/PEG-100 stearate 1.5%, cetearyl glucoside/cetearyl alcohol 1%, hydrogenated polydecene 2%, isononyl isononanoate 3%, polydimethylsiloxane 1%;

and C phase: 0.5 percent of p-hydroxyacetophenone;

phase D: preparation example 1 of the present invention wrinkle-removing and anti-aging composition 8%;

phase E: 0.5 percent of 1, 2-hexanediol.

The preparation process of the wrinkle-removing and anti-aging eye cream comprises the following steps:

(1) adding phase A into emulsifying pot, homogenizing for 3-5 min, heating to 85 deg.C, and stirring to dissolve completely.

(2) Adding the phase B into an oil phase pot, stirring and heating to 85 ℃, and stirring until the phase B is completely dissolved;

(3) when the A, B two phases reach the designated temperature, pumping the B phase into the A phase, stirring for 5 minutes, homogenizing for 3 minutes, adding the C phase, stirring and cooling;

(4) cooling to below 45 ℃, adding D, E phases, stirring uniformly, defoaming, reporting and checking to be qualified, and filtering and discharging by using 150-mesh filter cloth to obtain the wrinkle-removing and anti-aging eye cream.

EXAMPLE 4 preparation and application of wrinkle-removing anti-aging eye cream

The raw materials of the wrinkle-removing and anti-aging eye cream comprise:

and C phase: 0.5 percent of p-hydroxyacetophenone;

phase D: preparation example 3 of the present invention wrinkle-removing and anti-aging composition 8%;

phase E: 0.5 percent of 1, 2-hexanediol.

EXAMPLE 5 preparation and application of wrinkle-removing anti-aging cream

The raw materials of the wrinkle-removing and anti-aging cream comprise:

phase A: water (To 100%), carbomer 0.3%, xanthan gum 0.18%, glycerin 3%, disodium EDTA 0.01%;

phase B: glyceryl stearate/PEG-100 stearate 2%, cetearyl alcohol 2.5%, dimethicone 2.5%, neopentyl glycol diheptanoate 5%, caprylic/capric triglyceride 5%;

and C phase: 0.3 percent of arginine;

phase D: preparation example 1 of the present invention wrinkle-removing and anti-aging composition 10%;

phase E: 0.5 percent of 1, 2-hexanediol and 0.4 percent of phenoxyethanol/ethylhexyl glycerin.

The preparation process of the wrinkle-removing and anti-aging facial cream comprises the following steps:

(3) when the A, B two phases reach the designated temperature, pumping the B phase into the A phase, stirring for 5 minutes, and homogenizing for 3 minutes;

(4) adding the phase C into an emulsifying pot, stirring for 5 minutes, preserving heat for 15 minutes, and defoaming;

(5) cooling to below 45 ℃, adding D, E phases, stirring uniformly, reporting to check, filtering and discharging by using 150-mesh filter cloth to obtain the wrinkle-removing and anti-aging face cream.

EXAMPLE 6 preparation and application of wrinkle-removing anti-aging cream

The raw materials of the wrinkle-removing and anti-aging cream comprise:

and C phase: 0.3 percent of arginine;

phase D: preparation example 4 of the invention wrinkle-removing and anti-aging composition 10%;

Examples of the experiments

In order to better illustrate the advantages of the invention, the safety and efficacy of the composition obtained by the invention are tested and analyzed by adopting a specific cosmetic efficacy evaluation method aiming at a series of anti-wrinkle and anti-aging products of plant-derived anti-aging extracts (including an aconite leaf cowpea seed extract, a calanthe extract and an India swertia extract which have anti-aging mechanism and anti-aging efficacy similar to retinol).

1. Wrinkle-removing and anti-aging effects

The wrinkle-removing and anti-aging composition is tested by setting six sets of schemes and is used for screening out a composition system with the best effect in the invention,

the component settings are shown in table 1 below:

table 1 recipe content (%) -of each experimental group

	Composition 1	Composition 2	Composition 3	Composition 4	Composition 5	Composition 6
							Anti-aging extract of plant source	3	/	3	3	3	3
Palmitoyl pentapeptide-4	4	4	/	4	4	4
							Protein peptides	3	3	3	/	3	3
Rosemary extract	3	3	3	3	/	3
							Glycogen	1	1	1	1	1	/
Water (W)	Balance of	Balance of	Balance of	Balance of	Balance of	Balance of

The experimental method comprises the following steps:

30 testers were randomly selected and divided into 6 groups of 5. Before the test, the face is cleaned, the face is rested for 15 minutes at a fixed position, then the face area is tested by a VISIA machine of a skin tester, and the condition is recorded;

secondly, each group of testers respectively uses the essence containing the corresponding composition to massage until the essence is completely absorbed, the testers use the essence in the morning and at night every day, after using the essence for one month, a skin tester VISIA machine is used for testing the face area, and the condition is recorded.

The result is shown in fig. 1, after one month of trial, the wrinkle removing effect is better than that of other components when the essence containing the composition 1 is used, and therefore, the components of the wrinkle removing and anti-aging composition have better synergistic effect.

2. Evaluation of anti-wrinkle effect of aconite leaf cowpea seed extract

The experimental method comprises the following steps:

firstly, randomly extracting 20 testers, cleaning the face before testing, resting for 15 minutes at a fixed position, testing the facial area by using a VISIA machine of a skin tester, wherein lines in the figure show the number of wrinkles, and recording the situation;

② the wrinkle-removing and anti-aging eye cream of the embodiment 4 is smeared on the left eye, the wrinkle-removing and anti-aging eye cream of the embodiment 3 is smeared on the right eye, the eye cream is massaged until the eye cream is completely absorbed, the eye cream is used in the morning and evening every day, after one month of use, a facial area is tested by a skin tester VISIA machine, and the condition is recorded.

Results as shown in fig. 2, the fine lines of the left eye eyes were significantly reduced in comparison to the right eye eyes in the case of trial examples 3 and 4 after one month with only the aconite cowpea seed extract as a variable. The invention proves that the selected plant source anti-aging extract similar to the extract has ideal anti-wrinkle effect.

3. Evaluation of anti-wrinkle Effect of arginine/lysine polypeptide

The experimental method comprises the following steps:

firstly, randomly extracting 20 testers, cleaning the face before testing, resting for 15 minutes at a fixed position, testing the periocular region by using a skin tester VISIA machine, and recording the conditions;

secondly, the wrinkle-removing and anti-aging cream of the embodiment 6 is smeared on the left face, the wrinkle-removing and anti-aging cream of the embodiment 5 is smeared on the right face, the cream is massaged until the cream is completely absorbed, the cream is used in the morning and evening every day, after one month of use, a facial area is tested by a skin tester VISIA machine, green lines in the figure show the number of wrinkles, and the condition is recorded.

The results are shown in figure 3, and the one month post-trial examples 5 and 6 showed a significant reduction in the number of eye fine lines on the left face compared to the right face with only one variable of the arginine/lysine polypeptide. Proves that the selected arginine/lysine polypeptide has the effect of resisting wrinkles.

4. Evaluation of glycogen moisturizing Effect

The experimental method comprises the following steps:

first, 20 testers were randomly selected and divided into A, B groups of 10 testers each. Before testing, the face is cleaned, the skin is rested for 15 minutes at a fixed position, the skin is tested by using a skin moisture content tester, and data is recorded;

② the group A is applied with the wrinkle-removing and anti-aging essence liquid of the example 1, the group B is applied with the wrinkle-removing and anti-aging essence liquid of the example 2, the massage is carried out until the essence liquid is completely absorbed, the skin is tested by using a skin moisture content tester, and the data is recorded.

Data of A, B two groups of testers before and after the two groups of testers use the essence for 1 hour, 2 hours and 3 hours are averaged respectively, and then the moisturizing effect is evaluated through the data.

Results as shown in fig. 4, the skin hydration of the face of the test persons in group B was significantly higher than the skin hydration of the face of the test persons in group a with only glycogen for examples 1 and 2 after trial use. The glycogen selected by the scheme of the invention is proved to have ideal moisturizing effect.

5. Evaluation of antioxidant Effect of Rosemary extract

The experimental principle is as follows: DPPH assay is a simple method for screening free radical scavengers and evaluating antioxidant activity. The ethanol solution of DPPH is purple and the maximum absorption wavelength is 517 nm. When a free radical scavenger is added into the DPPH solution, the lone pair of electrons is paired, the absorption disappears or is weakened, and the solution becomes light in color and is yellow or light yellow, so that the effect of the free radical scavenger on resisting oxidation is proved.

The experimental method comprises the following steps:

preparing an experimental reagent: weighing a proper amount of DPPH, fixing the volume with absolute ethyl alcohol to obtain DPPH with the concentration of 0.1mmol/L, and storing the DPPH and the absolute ethyl alcohol solution at the temperature of 4 ℃ in a dark place;

② two test tubes are taken, and are added with the same amount of DPPH and absolute ethyl alcohol solution to be set as an experimental group, and the other test tube is set as a control group. In the test tubes of the experimental group, rosemary extract was added, shaken well and shaken well. The control group did not have any sample added.

As can be seen from FIG. 5, the color of the experimental group faded from dark purple to light yellow clear solution, which proves that the rosemary extract has antioxidant effect.

The above embodiments of the present invention are described in detail, and the principle and the implementation of the present invention are explained by applying specific embodiments, and the above description of the embodiments is only used to help understanding the method of the present invention and the core idea thereof; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.

Claims

1. The wrinkle-removing and anti-aging composition is characterized by comprising the following components in percentage by mass:

2. the wrinkle-removing and anti-aging composition as claimed in claim 1, wherein the plant-derived anti-aging extract includes cowpea seed extract of aconite, calanthe extract and/or swertia indica extract.

3. The wrinkle-removing and anti-aging composition according to claim 1 or 2, wherein the protein peptide polypeptide comprises arginine/lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate, and/or acetyl hexapeptide-8.

4. The wrinkle-removing and antiaging composition as claimed in any one of claims 1 to 3, characterized in that the glycogen is a product obtained by enzymatic reaction of non-transgenic waxy corn starch.

5. A method for preparing the wrinkle-removing and anti-aging composition according to any one of claims 1 to 4, comprising the step of adding a selected amount of the plant-derived anti-aging extract, palmitoyl pentapeptide-4, the protein peptide, rosemary extract and glycogen to water and mixing them thoroughly.

6. Use of the wrinkle-removing and anti-aging composition according to any one of claims 1 to 4 for the preparation of a cosmetic having wrinkle-removing and anti-aging effects.

7. A wrinkle-removing and anti-aging cosmetic comprising the wrinkle-removing and anti-aging composition according to any one of claims 1 to 4, and an acceptable excipient.

8. The wrinkle-removing and anti-aging cosmetic as claimed in claim 7, wherein the wrinkle-removing and anti-aging composition is contained in the cosmetic in an amount of 1-20 wt%.

9. The wrinkle-removing and anti-aging cosmetic according to claim 7 or 8, wherein the wrinkle-removing and anti-aging cosmetic comprises a wrinkle-removing and anti-aging essence, a wrinkle-removing and anti-aging eye cream, a wrinkle-removing and anti-aging mask patch, a wrinkle-removing and anti-aging lotion, or a wrinkle-removing and anti-aging cream.

10. A method for preparing the wrinkle-removing and anti-aging cosmetic as defined in any one of claims 7 to 9, which comprises the steps of preparing the desired wrinkle-removing and anti-aging composition according to the method of claim 5, and preparing the selected cosmetic category according to a conventional process by adding acceptable auxiliary materials.