CN115040448A - Skin care product with enhanced anti-aging effect and preparation method thereof - Google Patents

Abstract

The invention discloses a skin care product for strengthening anti-aging effect and a preparation method thereof. The small molecular peptide composition can promote the regeneration of skin collagen, the autophagy catalytic component can enhance the autophagy phenomenon of cells, and remove or degrade damaged cell structures, aged organelles and the like, and meanwhile, the autophagy metabolite also provides a raw material for the reconstruction of the cells, so that the cell activity is improved, and the aging condition of the skin is improved. The biological clock activating component can regulate the circadian rhythm of skin cells, so that the skin cells can realize self-repair at night, and the anti-aging effect is further enhanced.

Description

Skin care product with enhanced anti-aging effect and preparation method thereof

Technical Field

The invention relates to a skin care product for synchronously improving internal cause and external cause and enhancing anti-aging effect by activating biological clock and a preparation method thereof, in particular to a skin care product formula for synchronously improving internal cause and external cause and enhancing anti-aging effect by activating biological clock. The skin care product can be applied to the skin surface before rest at night except in the daytime, and because the skin care product contains a cell biological clock activating component, cells can still be in an active state when people rest at night, so that the anti-aging effect is further enhanced.

Background

The gradual loss of collagen from human skin with aging is an intrinsic factor causing skin aging, and the effective activation of skin collagen regeneration is an important method for resisting aging. The damage of ultraviolet rays, atmospheric pollution, electromagnetic radiation and the like to the skin is an external factor causing skin aging. These extrinsic factors often cause damage to the cytoplasm, organelles, mitochondria, cellular proteins, etc. of skin cells, resulting in the production of many fragmented materials that prevent the metabolic processes of healthy, undamaged cells.

In the face of fragmented substances generated by damaged cells, healthy cells can solve the harm caused by the damaged cells by means of autophagy, which is characterized in that the harmful cell fragments are usually phagocytosed in vacuoles and decomposed by lysozyme and the like, and the substances obtained by decomposition are amino acids and the like which can provide energy sources for the healthy cells and basic substances required by regeneration.

A biological clock gene refers to a gene that controls a biological rhythm. For example, the biological clock gene in skin cells can make the skin cells enter a rest state at night under normal conditions, and if external factors such as environmental stimulation and substance stimulation applied to the surface are used for stimulation, the skin cells can still keep an active state at night, and the self-repairing effect of the cells is enhanced through the autophagy function.

The anti-aging skin care product formula in the current market usually solves the internal factors of aging only by matching the single aging factor, such as moisturizing, nerve soothing, collagen regeneration stimulation and the like, and the invention realizes the solution of the skin aging problem by simultaneously solving the internal factors and the external factors, and further enhances the anti-aging effect by activating the biological clock factor and changing the circadian rhythm of the skin.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a skin care product for strengthening anti-aging effect and a preparation method thereof, and the skin care product based on the formula scheme has the characteristics of more obvious anti-aging effect and more complete efficacy by synchronously improving internal and external causes of skin aging and assisting in the activation of a biological clock.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows.

A skin care product for enhancing anti-aging effect comprises small molecule peptide composition, cell autophagy catalytic component, biological clock activating component, hydrolyzed algin, squalane, caffeine, bisabolol, lecithin, and hydroxydecyl ubiquinone.

Furthermore, the small molecule peptide composition comprises the components of tripeptide-1, acetyl hexapeptide-8 and palmitoyl pentapeptide-3.

Further, the cell autophagy catalytic component is extracted from yeast fermentation products, the yeast is usually bifidobacteria or lactobacilli, and the extract is generally extracted from the following plant fermentation products: cactus, ginseng, cola seed, Adenophora stricta and chamomile.

Further, the biological clock activating component is derived from echinacea purpurea extract, tripeptide-32, tetrapeptide-26 or mixtures thereof.

Further, the components in percentage by weight are as follows: small molecule peptide composition: tripeptide-1: 0.1-2%, acetyl hexapeptide-8: 0.1-2%, palmitoyl pentapeptide-3: 0.1-2%; autophagy catalytic components, mainly bifidobacteria fermentation lysates: 8% -20%; a biological clock activating component from echinacea purpurea extract: 0.1 to 1 percent; 0.2 to 2 percent of hydrolyzed algin, 0.2 to 5 percent of squalane, 0.2 to 2 percent of caffeine, 0.2 to 2 percent of bisabolol, 0.2 to 2 percent of lecithin, 0.2 to 1 percent of hydroxydecyl ubiquinone, 0.1 to 1 percent of cactus extract, 0.1 to 1 percent of ginseng extract, 0.1 to 1 percent of cola seed extract, 0.1 to 1 percent of Paeonia suffruticosa extract and 0.1 to 1 percent of chamomile extract;

further, the composition also comprises the following components: water, butylene glycol, 1, 3-propylene glycol, methyl gluceth-20, PEG/PPG-17/6 copolymer, glyceryl polyether-26, maltodextrin, bis-PEG-18 methyl ether dimethylsilane, arginine, sodium PCA, carbomer, disodium EDTA, xanthan gum, sodium hyaluronate, sorbitol, lecithin, oleic acid, hydrogenated lecithin, tocopheryl acetate, inulin lauryl carbamate, glycerol, p-hydroxyacetophenone, silicone triol alginate, 1, 2-hexanediol, cyanocobalamine, citric acid, sodium citrate.

A preparation method of a skin care product for strengthening anti-aging effect comprises the following steps:

(1) cleaning an emulsification pot, weighing water, butanediol, 1, 3-propylene glycol, glyceryl polyether-26, carbomer, EDTA disodium, xanthan gum, sodium hyaluronate, p-hydroxyacetophenone and 1, 2-hexanediol in proportion into a main phase pot, heating to 85 ℃, stirring, dispersing uniformly and dissolving completely;

(2) cooling to 45 ℃, adding bifidobacterium fermentation lysate, methyl glucitol polyether-20, PEG/PPG-17/6 copolymer, cyanocobalamine, citric acid, maltodextrin, sodium citrate, bis-PEG-18 methyl ether dimethylsilane, cola nut extract, glycerol, arginine, cactus extract, yeast fermentation product extract, ginseng root extract, sodium PCA, Adinandra berry pulp extract, tripeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-3, glycerol, butanediol, caffeine, bisabolol, lecithin, chamomile extract, purple coneflower extract, tocopheryl acetate, hydrolyzed algin and hydroxydecyl ubiquinone in sequence, stirring and dispersing uniformly;

(3) sampling, measuring pH, and discharging after the semi-finished product sampling detection meets the standard;

(4) and after the semi-finished products are inspected to be qualified, filling and packaging, and after the finished products are inspected to be qualified, warehousing.

Furthermore, the combined components of the tripeptide-1, the acetyl hexapeptide-8 and the palmitoyl pentapeptide-3 are small molecular peptides, wherein the tripeptide-1, also called as a dermal growth promoting factor, is a small molecular peptide consisting of three amino acids, also called as an oligopeptide, can act on the dermis layer, can promote the synthesis of extracellular matrixes such as collagen and glycosaminoglycan, strengthen the dermis layer, make the skin thicker and firmer and relieve wrinkles.

Acetyl hexapeptide-8, a neurotransmitter inhibitory peptide, is an oligopeptide that mimics the N-terminus of the SNAP-25 protein and consists of six amino acids. The anti-aging principle is that the anti-aging agent participates in competition of SNAP-25 protein at the site of a bubble complex, so that the formation of the bubble complex is influenced, and the bubble cannot effectively release neurotransmitter, so that neurotransmission muscle contraction information is blocked locally, muscle contraction is weakened, local muscles are relaxed, and the purpose of smoothing fine wrinkles is achieved.

Palmitoyl pentapeptide-3, which can penetrate the dermis to increase collagen, and retard the aging process of the skin through the reconstruction from inside to outside. The pentapeptide is actually a C-terminal fragment of the collagen I, and the pentapeptide is used for resisting wrinkles, directly stimulating the hyperplasia of the collagen, the elastic fiber and the hyaluronic acid, improving the water content and the water locking degree of the skin and increasing the thickness of the dermis layer to reduce fine lines.

The combined application of the three small molecular peptides can quickly and effectively promote the synthesis of skin collagen from the internal cause problem of skin aging, relieve the aging problem caused by real wrinkles and quickly smooth false wrinkles.

Further, the autophagy catalytic component is extracted from a yeast fermentation product, typically a binary split yeast fermentation product lysate, which contains fermented metabolites, cytoplasmic components, cell wall components and polysaccharide complexes. The active substance obtained by the biological fermentation technology can specifically stimulate and strengthen the autophagy function of skin cells, and strengthen the repair function of the cells to the damage caused by external factors such as ultraviolet rays, atmospheric pollution and electromagnetic radiation. The damaged cells are repaired, and the proportion of various mediums in the skin epidermis and the dermis cells is restored to be balanced.

The lysate of the yeast fermentation product can also prevent the release of an immunosuppressive factor IL-10 (interleukin 10) and prevent IL-12 (interleukin 12) from being inhibited, has catalytic and promoting effects on a gene repair mechanism, can further prevent the formation of apoptotic cells and maintain the integrity and the activity of the cells.

Further, the yeast fermentation product extract is generally extracted from the following plant fermentation products: cactus, ginseng, cola seed, Adenophora stricta and chamomile. The cactus extract is generally an extract of the stem of the prickly pear, contains various components such as polysaccharide substances, triterpene compounds, various amino acids required by human bodies, triterpene saponins, sterol and the like, has the function of promoting the proliferation of epidermal growth factors, has the function of activating glutathione reductase, can remove free radicals and effectively delay skin aging.

The ginseng extract is typically a ginseng root extract. The Ginseng radix extract contains various components such as ginsenoside, ginseng polysaccharide, bioactive peptide, etc., and has effects in increasing the content of superoxide dismutase and catalase in blood, and resisting oxidation. Partial monomer saponin in ginsenoside can reduce free radical content in vivo, delay nerve cell aging, stabilize cell membrane structure, increase protein synthesis, delay skin senilism, and prevent and reduce skin wrinkle.

The cola seed extract is typically sudan cola seed extract. The extract contains various components such as caffeine and glucoside, the antioxidant function of the caffeine can calm inflamed and inflamed skin, help to improve the overall health of the skin, resist aging and oxidation, and relieve edema by improving blood circulation, and the Sudan cola nut extract also contains theobromine, quercus tannatis, tannin, anthocyanin and the like, and can sterilize and resist inflammation, improve cell activity, astringe skin, whiten and remove spots. The contained glucoside and essential oil also have good moisturizing effect, can obviously enhance the moisturizing capability of the skin and enable the skin to be moist and glossy.

The feverfew extract is a feverfew extract. The extract of the feverfew contains various substances such as cyclic ethers, flavonoids, acids, triterpenes and the like. It has effects of inhibiting skin induced allergy, enhancing capillary resistance, inhibiting permeability, and relieving inflammation. The antioxidant can help skin to remove free radicals and relieve skin aging.

Further, the Echinacea purpurea extract, the tripeptide-32, the tetrapeptide-26 or the mixture thereof is usually extracted from petals of Echinacea purpurea, and the Echinacea purpurea is also named Echinacea purpurea and Echinacea purpurea, and the Echinacea purpurea extract contains multiple substances such as chicoric acid, polyphenol, polysaccharide, echinacoside and the like, the cichoric acid contained in the product is a biological clock gene activating component, the echinacoside contained in the product is a plant immune activator, has anti-inflammatory, antibacterial and antiviral effects and can enhance cellular immunity, humoral immunity and nonspecific immunity functions, the echinacoside is also a natural antioxidant, can clear away in-vivo free radicals and protect the organism from peroxidation damage, and the polyphenol, polysaccharide and other components contained in the product can inhibit hyaluronidase, reduce infiltration of inflammatory cells and inhibit cyclooxygenase, and have a certain inhibition effect on swelling and exudation caused by acute inflammation. Has certain inhibiting effect on melanocyte, and has effects of whitening skin and removing speckle.

Furthermore, the tripeptide-32 and the tetrapeptide-26 in the echinacea purpurea extract, the tripeptide-32 and the tetrapeptide-26 or the mixture thereof are used as biological clock gene activating components for regulating the biological clock rhythm of the skin, thereby effectively prolonging the self-repairing time of the skin and strengthening the anti-aging effect.

Further, the squalane in the combined formula contains "squalene" which is an ingredient existing in the skin itself, and is an important ingredient of human sebum and hydrophilic cell membranes which are natural protective barriers for the skin, and function as moisture-retaining agents and prevent the skin from being invaded by bacteria and fungi. The complete hydrophilic cell membrane can ensure the health of the skin and keep the skin soft and moist. The squalane is highly compatible with sebum secreted by skin, can remold the normal lipid structure of healthy skin of human body, moisturize and moisten the skin, effectively relieve dry skin and maintain the elasticity of the skin. The squalene can also rapidly repair skin cells, inhibit skin lipid peroxidation, promote proliferation of skin basal cells, delay skin aging, improve and eliminate chloasma, promote cell metabolism, and repair damaged cells.

Furthermore, the hydroxydecyl ubiquinone in the combined formula belongs to a highly lipophilic compound, is a derivative of coenzyme Q10, is smaller than coenzyme Q10 molecules and better absorbed, can effectively phagocytose free radicals causing skin organism aging, can not release the free radicals in an antioxidation process, can further prevent damage of external factors to the skin, delay the loss of skin collagen, provide energy required by skin metabolism, prevent the degradation of elastin fibers, and help the relaxed aging skin to quickly recover to a compact and traceless state, so that the aim of delaying aging is fulfilled.

Further, caffeine in the combination formula is a xanthine alkaloid compound. The skin moisturizing cream has the effects of resisting oxidation and inflammation and regulating skin moisture circulation in the formula.

The invention completely meets all indexes in the technical Specification for cosmetic safety (2022 edition) issued by the state, and the skin care product does not contain any toxic substance.

Due to the adoption of the technical scheme, the technical progress of the invention is as follows.

The invention adds the composition for promoting collagen synthesis, including tripeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-3, etc. into the formula to activate and strengthen the collagen synthesis and regeneration function of the skin, thereby relieving the skin aging from the endogenous angle. The yeast fermentation product extract, including the lysate of the yeast fermentation product, the yeast fermentation product extract and the like, is added into the formula to activate the autophagy function of skin cells, strengthen the self-repair mechanism of the skin cells to the damage of external environment, such as ultraviolet rays, atmospheric pollution, electromagnetic radiation and the like, and further relieve the skin aging from the external cause. The biological clock activating components including echinacea purpurea extract, tripeptide-32, tetrapeptide-26 and the like are added into the formula to regulate the biological clock rhythm of skin cells, so that the anti-aging effect of the skin care product on the skin is further enhanced, and the anti-aging effect of the product is more remarkable. The invention relates to a combined formula which is provided for the first time in the field of anti-aging skin care products and solves the problems from two aspects of internal cause and external cause, and enhances the anti-aging effect by assisting the activation of a biological clock, and the formula is water-soluble and is not limited by the dosage form of the product, can be used for products such as aqua, emulsion, cream, essence and the like, and fills the blank of products sold in the market.

Detailed Description

The skin care product for strengthening the anti-aging effect is prepared by synchronously improving the internal cause and the external cause of skin aging and assisting in improving the anti-aging effect in a biological clock activation mode according to the raw materials in percentage by weight in the table 1.

TABLE 1

The preparation method of the skin care product comprises the following steps:

(1) cleaning the emulsifying pot, weighing phase A into the main phase pot, heating to 85 ℃, stirring and dispersing uniformly, adding phase B, stirring and dispersing uniformly, and dissolving completely.

(2) Cooling to 45 ℃, adding the phase C in turn, stirring and dispersing uniformly.

(3) Sampling, measuring pH, and discharging after the semi-finished product sampling detection meets the standard.

(4) And after the semi-finished products are inspected to be qualified, filling and packaging, and after the finished products are inspected to be qualified, warehousing.

The present invention will be described in further detail with reference to specific embodiments.

Example 1

In this example, the components of the skin care product are shown in table 2.

TABLE 2

Example 2

In this example, the components of the skin care product are shown in table 3.

TABLE 3

Example 3

In this example, the skin care product components are shown in table 4.

TABLE 4

The skin care products prepared in the three examples were tested and tested for efficacy and compared to some well-known foreign brand of skin care products commercially available.

1) Skin smoothness change rate test

Finding 10 female volunteers aged 18-50 years, respectively using the anti-aging and anti-aging skin care products with the formula on the face, using the skin care products once a day for 30 days continuously, and not using any other products during the test period. The smoothness data is obtained comprehensively by using a VISIA facial image analyzer under the conditions that the ambient temperature is 25 +/-1 ℃ and the humidity is 50 +/-5%, and the higher the data value is, the worse the skin smoothness is. The test results are shown in Table 5.

TABLE 5

Sample (I)	Before use	After use
			Example 1	3.2332	1.3021
Example 3	3.0353	1.8595
			Some famous foreign brand product on the market	3.084	1.9972

The experimental results show that the skin smoothness of the invention is better than the skin smoothness of a certain famous foreign brand skin care product sold in the market, and the anti-aging effect is more obvious.

2) Test for promoting collagen synthesis

Human fibroblasts are cultured by the skin care products of the embodiment and a certain well-known foreign brand skin care product nutrient solution sold in the market, then the synthetic amount of collagen is measured, the synthetic amount data of the collagen is comprehensively obtained, and the higher the data value is, the better the collagen synthesis promoting capability is. The test results are shown in Table 6.

TABLE 6

Sample (I)	Example 1	Example 2	Example 3	Some famous foreign brand product on the market
					Collagen synthesis amount%	33	28	25	21

The experimental results show that the collagen synthesis promoting ability of the invention is better than that of a certain skin care product of a known foreign brand on the market.

3) Anti-aging test

The skin care products of the examples and the commercially available skin care product nutrient solutions of certain well-known foreign brands were used to culture caenorhabditis elegans, and then the skin care products of the examples and the commercially available products of certain well-known foreign brands were examined for their effect on the longevity of the model organism caenorhabditis elegans. The test results are shown in Table 7.

TABLE 7

Sample(s)	Average life (sky)	Maximum life (sky)
			Example 1	14.89±0.45d	26.26±1.27d
Example 2	13.77±0.37d	22.26±1.27d
			Example 3	12.89±0.45d	19.26±1.27d
Some famous foreign brand product on the market	11.97±0.55d	17.33±1.07d

The experimental results show that the anti-aging capability of the invention is better than that of a certain famous foreign brand product sold in the market.

4) Reduced expression line testing

Finding 10 female volunteers aged between 40-60 years, respectively using the anti-aging skin care product of the embodiment and a certain commercially-known foreign brand product on the forehead, using the skin care product twice a day for 28 days continuously, and not using any other product during the test period. The VISIA facial image analyzer is adopted to comprehensively obtain wrinkle reduction rate data compared with the first day under the conditions that the ambient temperature is 25 +/-1 ℃ and the humidity is 50 +/-5%, wherein the higher the wrinkle reduction rate is, the better the ability of reducing expression lines is. The test results are shown in Table 8.

TABLE 8

Sample (I)	Wrinkle reduction Rate (%)
		Example 1	57
Example 2	51
		Example 3	45
Some famous foreign brand product on the market	42

The experimental results show that the ability of reducing the expression pattern is better than that of a certain famous foreign brand product sold in the market.

5) Skin elasticity test

Finding 10 female volunteers aged 28-50 years, respectively using the anti-aging skin care products of the embodiment and a certain commercially-known foreign brand product on the face, continuously using for 4 weeks, testing by a German MAP580 skin tester, and comprehensively obtaining the stretching amount Ue of the positioning elastic part, wherein the higher the data value is, the better the skin elasticity is. The test results are shown in Table 9.

TABLE 9

Sample (I)	Before use	After 2 weeks of use	After 4 weeks of use
				Example 1	0.257	0.385	0.566
Example 2	0.255	0.354	0.495
				Example 3	0.261	0.327	0.493
Some famous foreign brand product on the market	0.252	0.289	0.458

The experimental results show that the skin elasticity recovery effect of the invention is better than that of a certain famous foreign brand product sold in the market.

6) Epidermal and dermal layer adhesion test

Finding 10 female volunteers aged 28-50 years, respectively using the anti-aging skin care product of the embodiment and a certain commercially-known foreign brand product on the inner side of the arm, continuously using for 7 days, soaking the skin for 2 hours with 5% SDS lotion before testing, then testing the TEWL transdermal water loss rate by using a Tewameter tester compared with the test, and comprehensively obtaining the TEWL transdermal water loss rate, wherein the lower the data value is, the better the adhesion degree of the epidermis and the dermis is. The test results are shown in Table 10.

Watch 10

Sample (I)	TEWL transdermal water loss%
		Example 1	200
Example 2	300
		Example 3	500
Some famous foreign brand product on the market	600

The results of the experiments show that the adhesion degree of the epidermis and the dermis of the invention is more obvious than that of the epidermis and the dermis of a certain famous foreign brand product sold in the market.

7) Anti-glycation assay

15 female volunteers aged 18-50 years were found, and the anti-aging skin care products of the above examples and some well-known foreign brand products were applied to the face, respectively, twice a day for 30 days without any other products during the test period. The glycation inhibition results obtained from the measurements using the german MAP580 skin tester are shown in table 11.

TABLE 11

Sample (I)	Example 1	Example 2	Example 3	Some famous foreign brand product on the market
					Glycation inhibition (%)	57	51	48	45

The experimental results show that the anti-saccharification capacity of the invention is more obvious than the anti-saccharification effect of a certain famous foreign brand product sold in the market.

The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and there may be some slight structural changes in the implementation, and if there are various changes or modifications to the present invention without departing from the spirit and scope of the present invention, and within the claims and equivalent technical scope of the present invention, the present invention also includes those changes and modifications.

Claims (7)

1. A skin care product for strengthening anti-aging effect is characterized in that: comprises small molecule peptide composition, cell autophagy catalytic component, biological clock activating component, hydrolyzed algin, squalane, caffeine, bisabolol, lecithin, and hydroxydecyl ubiquinone.

2. The anti-aging effect-potentiated skin care product of claim 1, which is characterized in that: the small molecular peptide composition comprises the components of tripeptide-1, acetyl hexapeptide-8 and palmitoyl pentapeptide-3.

3. The anti-aging effect-potentiated skin care product of claim 1, which is characterized in that: the cell autophagy catalytic component is extracted from yeast fermentation products, the yeast is usually bifidobacteria or lactobacilli, and the extract is generally extracted from the following plant fermentation products: cactus, ginseng, cola nut, Adiantum montanum, and Sphallerocarpus gracilis.

4. The anti-aging effect-potentiated skin care product of claim 1, which is characterized in that: the biological clock activating component is selected from Echinacea purpurea extract, tripeptide-32, tetrapeptide-26 or their mixture.

5. The anti-aging effect-potentiated skin care product of claim 1, which is characterized in that: comprises the following components in percentage by weight:

small molecule peptide composition, tripeptide-1: 0.1-2%, acetyl hexapeptide-8: 0.1-2%, palmitoyl pentapeptide-3: 0.1-2%;

autophagy catalytic components, mainly bifidobacteria fermentation lysates: 8% -20%;

a biological clock activating component from echinacea purpurea extract: 0.1 to 1 percent;

0.2 to 2 percent of hydrolyzed algin, 0.2 to 5 percent of squalane, 0.2 to 2 percent of caffeine, 0.2 to 2 percent of bisabolol, 0.2 to 2 percent of lecithin, 0.2 to 1 percent of hydroxydecyl ubiquinone,

0.1 to 1 percent of cactus extract, 0.1 to 1 percent of ginseng extract, 0.1 to 1 percent of cola nut seed extract, 0.1 to 1 percent of Adansonia dubia extract and 0.1 to 1 percent of chamomile extract.

6. The anti-aging effect-enhancing skin care product of claim 1, further comprising the following components: water, butylene glycol, 1, 3-propylene glycol, methyl gluceth-20, PEG/PPG-17/6 copolymer, glyceryl polyether-26, maltodextrin, bis-PEG-18 methyl ether dimethylsilane, arginine, sodium PCA, carbomer, disodium EDTA, xanthan gum, sodium hyaluronate, sorbitol, lecithin, oleic acid, hydrogenated lecithin, tocopheryl acetate, inulin lauryl carbamate, glycerol, p-hydroxyacetophenone, silicone triol alginate, 1, 2-hexanediol, cyanocobalamine, citric acid, sodium citrate.

7. A method for preparing the anti-aging effect-potentiated skin care product according to any one of claims 1 to 6, comprising the steps of:

(1) cleaning an emulsification pot, weighing water, butanediol, 1, 3-propylene glycol, glyceryl polyether-26, carbomer, EDTA disodium, xanthan gum, sodium hyaluronate, p-hydroxyacetophenone and 1, 2-hexanediol in proportion into a main phase pot, heating to 85 ℃, stirring, dispersing uniformly and dissolving completely;

(2) cooling to 45 ℃, adding bifidobacterium fermentation lysate, methyl glucitol polyether-20, PEG/PPG-17/6 copolymer, cyanocobalamine, citric acid, maltodextrin, sodium citrate, bis-PEG-18 methyl ether dimethylsilane, cola nut extract, glycerol, arginine, cactus extract, yeast fermentation product extract, ginseng root extract, sodium PCA, Adinandra berry pulp extract, tripeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-3, glycerol, butanediol, caffeine, bisabolol, lecithin, chamomile extract, purple coneflower extract, tocopheryl acetate, hydrolyzed algin and hydroxydecyl ubiquinone in sequence, stirring and dispersing uniformly;

(3) sampling, measuring pH, and discharging after the semi-finished product sampling detection meets the standard;

(4) and after the semi-finished products are inspected to be qualified, filling and packaging, and after the finished products are inspected to be qualified, warehousing.