CN111388346A

CN111388346A - Freeze-dried powder live mask with vegetable protein and polypeptide formula and preparation method thereof

Info

Publication number: CN111388346A
Application number: CN202010466718.5A
Authority: CN
Inventors: 杨跃飞; 刘元柏
Original assignee: Shanghai Jieli Biotechnology Co ltd
Current assignee: Shanghai Jieli Biotechnology Co ltd
Priority date: 2020-05-28
Filing date: 2020-05-28
Publication date: 2020-07-10

Abstract

The invention relates to the field of skin care cosmetics, in particular to a freeze-dried powder live mask with a formula of vegetable protein and polypeptide and a preparation method thereof. More specifically, the formula of the plant protein and polypeptide biochemical active substances is optimized, the plant protein and polypeptide biochemical active substances in the formula are directly freeze-dried on a mask cloth to prepare a freeze-dried powder mask by utilizing the activity protection technology of the biochemical active substances, namely the vacuum freeze-drying technology and a specific freeze-drying process, and are not directly added into essence, the package is separated by adopting films and liquids, and the films and liquids are dissolved immediately after use, so that the biochemical activity of the plant protein and polypeptide components in the formula is effectively protected, the preservative is not added, and the technical performance, the efficacy and the safety of the mask are greatly improved; meanwhile, the living mask provided by the invention has quick effect, takes effect quickly after being used, and is particularly suitable for skin moisturizing, barrier repairing, wrinkle resisting and aging resisting.

Description

Freeze-dried powder live mask with vegetable protein and polypeptide formula and preparation method thereof

Technical Field

The invention relates to the field of skin care cosmetics, in particular to a freeze-dried powder live mask with a formula of vegetable protein and polypeptide and a preparation method thereof.

Background

The Mask is widely used because of its good effects of instantly moisturizing water, nourishing, soothing, anti-wrinkle, anti-aging, after-sun repair, etc., and is the most popular of skin care cosmetics. Among them, the "cloth-on-film" is the most common. The "mask cloth mask" is a mask which is used by impregnating non-woven fabrics of different base materials with essence and then applying the essence to the face. Therefore, the essence plays an important role in the effect of the mask. With the progress of modern cosmetic technology, more and more 'biochemical active substances' with high technology, high performance (efficacy) and high safety are continuously developed and applied to the formula of facial mask essence, such as 'natural proteins', 'beauty polypeptides', 'plant polysaccharides', 'vitamins' and the like. However, the currently available masks have at least three disadvantages in terms of the application of these "biochemical actives": (1) more and stronger chemical preservatives are added to prevent the high nutrition and easy deterioration. (2) The problems of easy oxidation, illumination or compatibility inactivation exist. (3) The active content is low, and the efficacy is not exerted remarkably, which is related to the first two factors. How to effectively promote the application of the biochemical active substances in the membrane cloth and the membrane mask becomes a problem of general attention of the technical field in the industry.

Disclosure of Invention

Aiming at some problems in the prior art of 'membrane cloth mask', the invention provides a high-end anti-aging mask which is based on a modern high-performance (efficacy) 'biochemical active matter' formula and has the effects of lasting moisture preservation, sensitive repair, firming and anti-wrinkle, in particular to a freeze-dried powder live mask which is prepared from vegetable protein and polypeptide, and comprises freeze-dried powder, a mask cloth and an essence solution solvent; the freeze-dried powder comprises the following raw materials, by weight, 5-8 parts of hydrolyzed plant protein, 3-15 parts of an excipient, 0.2-1.3 parts of polypeptide and 100 parts of deionized water; the excipient comprises, by weight, 2.5-6 parts of mannitol, 1-5 parts of pullulan and 0.05-0.2 part of sodium hyaluronate.

As a preferable technical scheme of the invention, the molecular weight of the sodium hyaluronate is 80-130 ten thousand daltons.

According to a preferable technical scheme, the polypeptide comprises, by weight, 0.1-0.3 part of carnosine, 0.03-0.5 part of oligopeptide-1, 0.01-0.3 part of acetyl hexapeptide-8 and 0.05-0.2 part of palmitoyl pentapeptide-4.

According to a preferable technical scheme, the essence solution solvent comprises, by weight, 8-41 parts of a skin conditioner, 2.5-7 parts of a humectant, 0.1-0.3 part of an anti-inflammatory agent, 0.1-1 part of a chelating agent, and 100 parts of deionized water.

According to a preferable technical scheme, the skin conditioner comprises, by weight, 0.03-0.1 part of poly gamma-sodium glutamate, 1-5 parts of glycerol glucoside, 1-5 parts of yeast- β glucan, 1-5 parts of betaine, 1-5 parts of nicotinamide and 4-20 parts of plant extract.

As a preferred embodiment of the present invention, the plant extract includes carob seed extract, honey extract, asiatic centella root extract, and baical skullcap root extract.

As a preferable technical scheme, the plant extract comprises, by weight, 1-5 parts of carob seed fruit extract, 1-5 parts of honey extract, 1-5 parts of asiatic centella root extract and 1-5 parts of baical skullcap root extract.

As a preferred technical scheme of the invention, the chelating agent comprises caprylyl hydroximic acid and alcohols according to the parts by weight.

As a preferable embodiment of the present invention, the hydrolyzed vegetable protein includes at least one of hydrolyzed soy protein, hydrolyzed corn protein, and hydrolyzed wheat protein.

The second aspect of the invention provides a preparation method of the freeze-dried powder live mask with the vegetable protein and polypeptide formula, which comprises the following steps:

(1) the preparation of the freeze-dried powder mask comprises the steps of keeping the temperature of deionized water for sterilization, adding an excipient at 80 ℃, stirring for dissolving uniformly, adding polypeptide and hydrolyzed plant protein at 30 ℃, stirring for mixing for dissolving uniformly, filtering to obtain freeze-dried liquid, soaking the freeze-dried liquid into a mask cloth with the amount of 10-15 m L/piece for saturation and no liquid dripping, then filling the mask cloth into a freeze-drying tray, and performing vacuum freeze-drying treatment in a freeze dryer to obtain the freeze-dried powder mask;

(2) preparing essence solution solvent: and after the deionized water is subjected to heat preservation and sterilization, respectively adding the skin conditioner, the humectant, the anti-inflammatory agent and the chelating agent, and then mixing and stirring uniformly to obtain the essence solvent.

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, a formula of a plant protein and polypeptide biochemical active substance is optimized, and the biochemical active substance is directly and uniformly freeze-dried on the mask cloth by utilizing the activity protection technology of the biochemical active substance, namely the vacuum freeze-drying technology and a specific freeze-drying process, rather than being directly added into essence, and the package is separated by adopting films and liquids, namely the films and the liquids are dissolved immediately after use, so that the activity of the biochemical active substance component of the stable state freeze-dried powder formed on the film cloth is effectively protected, no preservative is added, and the technical performance, the efficacy and the safety of the mask are greatly improved; meanwhile, the living mask provided by the invention has the advantages of quick effect, obvious effect after use, and is particularly suitable for people with dry, yellow and dull, dark, sensitive, loose and fine lines on skin, and can repair the skin and resist aging.

Drawings

FIG. 1 is a diagram showing the moisturizing effect of a facial mask soaked and dissolved by essence solvents and freeze-dried powder obtained in example 3 of the invention and a control sample after being applied for 1 time;

FIG. 2 is a diagram showing the moisturizing effect of the facial mask and the control sample which are soaked and dissolved by the essence solution solvent and the freeze-dried powder facial mask obtained in example 3 of the invention after being continuously applied for 5 days and stopped for 3 days;

FIG. 3 is a graph showing the repairing effect of the facial mask prepared by soaking and dissolving the essence solution solvent and the freeze-dried powder facial mask and the control sample on skin barrier damage in example 3;

FIG. 4 is a graph showing the effect of the facial mask prepared by soaking and dissolving the essence solution solvent and the freeze-dried powder facial mask and the control sample on improving the skin elasticity, obtained in example 3;

fig. 5 is a graph showing the effect of the facial mask soaked and dissolved by the essence solvent and the freeze-dried powder facial mask obtained in example 3 on fine wrinkles.

Detailed Description

The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.

The invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which comprises freeze-dried powder, mask cloth and an essence solvent.

Freeze-dried powder

In one embodiment, the freeze-dried powder is prepared from 5-8 parts of hydrolyzed plant protein, 3-15 parts of excipient, 0.2-1.3 parts of polypeptide and deionized water by weight, and the balance is 100 parts.

In one embodiment, the excipient comprises 2.5-6 parts of mannitol, 1-5 parts of pullulan and 0.05-0.2 part of sodium hyaluronate by weight.

Preferably, the excipient comprises 3 parts of mannitol, 1.5 parts of pullulan and 0.1 part of sodium hyaluronate according to parts by weight. In one embodiment, the raw materials for preparing the freeze-dried powder comprise 7 parts of hydrolyzed vegetable protein, 4.6 parts of excipient, 0.72 part of polypeptide and deionized water to make up to 100 parts.

In one embodiment, the hydrolyzed vegetable protein comprises at least one of hydrolyzed soy protein, hydrolyzed corn protein, and hydrolyzed wheat protein.

Preferably, the hydrolyzed vegetable protein is hydrolyzed soy protein.

In one embodiment, the molecular weight of the sodium hyaluronate is 80 to 130 ten thousand daltons.

The sodium hyaluronate has a molecular weight of 80-130 ten thousand daltons, is prepared by a fermentation method, is a natural efficient humectant widely used in cosmetics, and can repair dryness and enhance the skin moisturizing barrier function.

The hydrolyzed soybean vegetable protein is rich in various active substances such as amino acids, soybean isoflavone and the like which are necessary for human bodies, and has the effects of moisturizing, oxidation resistance, pollution resistance and the like.

The mannitol, also called mannitol, is prepared from starch sugar or sucrose as a raw material, and is a commonly used humectant for medicines and cosmetics. Is used as the main excipient of the freeze-dried powder in the invention.

The pullulan in the invention is natural polysaccharide obtained by fermenting starch through natural yeast, and has the effects of moisture retention, oxidation resistance and the like. In the invention, the compound is used as an auxiliary excipient of the freeze-dried powder.

In one embodiment, the polypeptide comprises 0.1-0.3 parts of carnosine, 0.03-0.5 parts of oligopeptide-1, 0.01-0.3 parts of acetyl hexapeptide-8 and 0.05-0.2 parts of palmitoyl pentapeptide-4 by weight.

Preferably, the polypeptide comprises 0.2 part of carnosine, 0.3 part of oligopeptide-1, 0.15 part of acetyl hexapeptide-8 and 0.07 part of palmitoyl pentapeptide-4 in parts by weight.

The carnosine in the invention is a natural endogenous dipeptide, is widely present in muscles, brains and hearts of mammals, and has wide pharmacological activity. Carnosine is proved to have strong free radical scavenging, antioxidant and anti-glycosylation, and effective nursing ingredients capable of effectively protecting skin collagen, dispelling yellow, whitening, resisting aging and maintaining the skin in a young state.

The oligopeptide-1 is prepared by an escherichia coli fermentation method or an amino acid biochemical synthesis method. The protein is a water-soluble non-glycosylated micromolecule protein, contains 23 amino acid residues, has the effects of promoting epidermal cell regeneration, accelerating the renewal of aged cells, synergistically resisting aging and the like, and makes the skin smooth and tender.

The acetyl hexapeptide-8 in the invention is a modern bioactive polypeptide, also called neurotransmitter inhibitory peptide, and has the obvious effects of tightening, resisting wrinkles, increasing elasticity and resisting aging.

The palmitoyl pentapeptide-4 is a modern bioactive polypeptide, is an important signal peptide for regulating the activity of cells and activating certain genes involved in the processes of extracellular matrix renewal and cell proliferation, and has the effects of promoting the generation of type I and III collagen and fibronectin, thereby increasing the thickness of skin and reducing fine wrinkles.

The oligopeptide-1 is purchased from Angel biotechnology limited company in Shenzhen.

The sodium hyaluronate with the molecular weight of 80-130 ten thousand daltons is purchased from Shandong Huaxinfuruida biomedicine Limited company.

The palmitoyl pentapeptide-4 is purchased from Zhejiang surge tide biotechnology limited.

The hydrolyzed soy protein of the present invention is produced by Vincience, France, and is available from Shanghai Dry fly chemical Co., Ltd.

The mannitol of the invention is purchased from Guangxi Nanning chemical pharmaceutical GmbH.

The pullulan of the present invention was purchased from Korea International trade (Shanghai) Co.

Mask cloth

In one embodiment, the mask cloth is selected from any one of cotton film cloth, silk mask cloth and polyester mask cloth.

The mask cloth is cotton film cloth.

Essence solution solvent

In one embodiment, the essence solution solvent comprises, by weight, 8-41 parts of a skin conditioner, 2.5-7 parts of a humectant, 0.1-0.3 part of an anti-inflammatory agent, 0.1-1 part of a chelating agent, and deionized water to make up to 100 parts.

< skin Conditioning Agents >

In one embodiment, the skin conditioner comprises 0.03-0.1 part of poly gamma-sodium glutamate, 1-5 parts of glycerol glucoside, 1-5 parts of yeast- β glucan, 1-5 parts of betaine, 1-5 parts of nicotinamide and 4-20 parts of plant extract by weight.

In one embodiment, the plant extract includes carob seed fruit extract, honey extract, centella asiatica root extract, scutellaria baicalensis root extract; preferably, the plant extract comprises 1-5 parts by weight of carob seed extract, 1-5 parts by weight of honey extract, 1-5 parts by weight of centella asiatica root extract and 1-5 parts by weight of scutellaria baicalensis root extract; more preferably, the plant extract comprises 2 parts by weight of carob seed extract, 2 parts by weight of honey extract, 2 parts by weight of centella asiatica root extract, 2 parts by weight of scutellaria baicalensis root extract.

In a preferred embodiment, the skin conditioner comprises, by weight parts, 0.04 parts poly gamma-sodium glutamate, 1.5 parts glycerol glucoside, 2 parts yeast- β glucan, 2 parts betaine, 2 parts niacinamide, 8 parts plant extract.

The poly-gamma-sodium glutamate is a ferment prepared by fermenting soybeans, is called natto gum and is abbreviated as gamma-PGA. The natural moisturizing factor NMF can obviously promote the proliferation of the natural moisturizing factor NMF in skin keratinocytes, can permeate and infiltrate the stratum corneum, stimulate activated cells, and enable the skin to have more transparency and lubrication feeling, and is a functional long-acting moisturizing, skin barrier enhancing protective agent and skin feeling lubricant, but not a general coverage type moisturizing agent.

The glycerol glucoside is extracted from salt-tolerant blue-green algae (cyanobacteria) or a plant (immortal grass) growing in an African desert, is a natural cell activator, and has the effects of enhancing the activity and metabolism of skin cells, activating cell regeneration, enhancing the oxidation resistance (SOD) of the skin cells, accelerating wound healing and tissue repair, and increasing the moisture retention, elasticity and smoothness of skin.

The yeast β -glucan in the invention is a polysaccharide extracted from fungi organisms such as mushrooms, tremella, lucid ganoderma, yeast and the like, has the main active component of β - (1, 3) -D-glucan, has the functions of immunity enhancement and detoxification, and has good effects on UV sunburn repair, wound healing, anti-allergy and the like.

The betaine in the invention is extracted from beet root and mainly contains plant amino acid, namely trimethylglycine. Has long-acting moisturizing and anti-allergy repairing effects, provides sufficient water and nutrients for the horny layer, activates cells, and enhances moisturizing and barrier functions.

The nicotinamide of the invention is also called vitamin B3 or vitamin PP. The skin care product has the effects of promoting the synthesis of two most important proteins, namely epidermal barrier envelope protein and filaggrin, and increasing the regeneration quantity of collagen and fibroblasts in a dermis layer, so that the skin is soft, moist and compact, the dryness and roughness are prevented, the skin is whitened, and the skin color is brightened.

The honey extract is prepared from natural honey by a biological fermentation method, and is rich in gluconic acid. Has the effects of removing aged cutin (dead skin), promoting epidermal cell renewal, and stimulating dermal cell activity, thereby rejuvenating and whitening skin.

The carob fruit extract of the invention is extracted from wild carob, and the main component is galactomannan oligosaccharide. It can provide accelerated regulation for the repairing process of damaged skin caused by various factors and enhance the repairing effect.

The asiatic centella root extract is extracted from asiatic centella, and has the main active components of asiaticoside and madecassoside, so that the asiaticoside has excellent repairing effect on skin damage, allergy and scar.

The radix scutellariae extract in the invention is a traditional Chinese herbal medicine and is rich in physiologically active substances such as baicalin, baicalein and the like. The skin care product has the functions of strong oxidation and Ultraviolet (UV) resistance, and can effectively repair the damage of environmental pollution (automobile exhaust, industrial waste gas, tobacco smoke and the like) and psychological pressure (overload work, interpersonal relationship and the like) to the skin and prevent aging phenomena such as relaxation, wrinkling and the like.

< humectant >

In one embodiment, the moisturizer includes, but is not limited to, xylitol, glycerin, sodium hyaluronate, 1, 2-pentanediol, 1, 3-propanediol, hexylene glycol, polyethylene glycol, sorbitol.

In one embodiment, the humectant comprises glycerin and 1, 2-pentanediol.

The humectant comprises 2-5 parts by weight of glycerol and 0.5-2 parts by weight of 1, 2-pentanediol; preferably, the humectant comprises 3 parts by weight of glycerin and 1.2 parts by weight of 1, 2-pentanediol.

The glycerin is a commonly used humectant for cosmetics of plant origin and has a solvent, so that the solubility and the transparency of the formula are improved.

The 1, 2-pentanediol in the invention is a safe humectant with a bacteriostatic function, and can enhance the preservative function of the formula.

< anti-inflammatory Agents >

In one embodiment, the anti-inflammatory agent may be exemplified by dipotassium glycyrrhizinate, diammonium glycyrrhizinate, allantoin, and the like.

Preferably, the anti-inflammatory agent is dipotassium glycyrrhizinate.

< chelating agent >

In one embodiment, the chelating agent comprises caprylhydroxamic acid and alcohols.

In a preferred embodiment, the chelating agent comprises caprylhydroxamic acid, hexylene glycol, and 1, 3-propanediol.

Preferably, the chelating agent comprises 0.005-0.05 part by weight of caprylyl hydroximic acid, 0.03-0.3 part by weight of hexanediol and 0.065-0.65 part by weight of 1, 3-propanediol; more preferably, the chelating agent comprises 0.04 parts by weight of caprylhydroxamic acid, 0.24 parts by weight of hexylene glycol, and 0.52 parts by weight of 1, 3-propanediol.

The chelating agent has good antibacterial effect, is very mild and non-irritant, and has an antiseptic and stabilizing effect on a system.

In a preferred embodiment, the essence vehicle comprises 15.54 parts by weight of skin conditioner, 4.2 parts by weight of humectant, 0.2 part by weight of anti-inflammatory agent, 0.8 part by weight of chelating agent and deionized water to make up 100 parts by weight. The deionized water in the invention is prepared by the tap water through two-stage Reverse Osmosis (RO) and Electric Desalting (EDI). The product is sterilized at 90 deg.C for 30 min before use.

The honey extract of the invention is purchased from Songbin commercial chemical industry (Shanghai) Co.

The carob fruit extract of the present invention is produced by rubia cordifolia (SI L AB) gmbh, france, and is available from shanghai fangkai mao development ltd.

The centella asiatica root extract of the present invention is available from SABINSA corporation, usa.

The extract of scutellaria root of the present invention is available from the company of ICHIMARU (ICHIMARU PHARCOS).

The freeze-dried powder and the essence solution have different weight part standards; when the freeze-dried powder and the essence solution solvent are prepared respectively, the weight part standards of the components in the freeze-dried powder and the essence solution are the same.

The invention puts gamma-sodium glutamate, glycerol glucoside, yeast β -glucan, betaine, honey extract, nicotinamide and carob fruit extract into essence, aims to increase the biological activity of plant protein and polypeptide freeze-dried powder on the mask cloth, exert positive synergistic effect, supplement each other and mutually promote, can update the stratum corneum, enhance the tolerance of the skin to external damage, stimulate the self-healing activity of the skin barrier, further prevent water loss, resist the damage of external Ultraviolet (UV), pollutants and other adverse factors, and realize the long-acting moisturizing, repairing stimulation and sensitivity, tightening and delicate skin care effects.

(1) the preparation of the freeze-dried powder mask comprises the steps of keeping the temperature of deionized water for sterilization, adding an excipient at 80 ℃, stirring for dissolving uniformly, adding polypeptide and hydrolyzed plant protein at 30 ℃, stirring for mixing for dissolving uniformly, filtering to obtain freeze-dried liquid, soaking the freeze-dried liquid into a mask cloth with the amount of 10-15 m L/piece for saturation and no liquid dripping, filling the mask cloth into a freeze-drying tray, and performing vacuum freeze-drying treatment in a freeze dryer to obtain the freeze-dried powder mask;

In one embodiment, the preparation method of the freeze-dried powder live mask of the plant protein and polypeptide formula comprises the following steps:

(1) preparing a freeze-dried powder mask:

1. adding deionized water into a vacuum homogenizing emulsifying pot at room temperature, heating to 85-90 ℃, preserving heat for 30 minutes for sterilization, then cooling to 80 ℃, sequentially adding mannitol, pullulan and sodium hyaluronate, and stirring for dissolving uniformly;

2. cooling to below 30 deg.C under stirring, sequentially adding carnosine, oligopeptide-1, acetyl hexapeptide-8, and palmitoyl pentapeptide-4, hydrolyzing vegetable protein, stirring for 10 min after adding, mixing completely and dissolving uniformly, filtering and discharging to obtain lyophilized solution;

3. soaking 10-15 ml/piece of folded cotton mask cloth into a freeze-drying liquid with saturated liquid absorption and no liquid dropping quantitatively, and then loading into a freeze-drying tray;

4. putting the freeze-drying plate into a vacuum freeze-drying machine, starting the freeze-drying machine, setting freeze-drying process parameters, freezing the membrane cloth from room temperature to-50 ℃, and maintaining for 3-4 hours to fully freeze the freeze-drying liquid on the mask cloth;

5. gradually heating the frozen and compacted mask cloth from-50 ℃ to-25 ℃, wherein the heating rate is about 0.17-1 ℃/min, the temperature is maintained for 12-16 hours, and the vacuum degree is 0.01 mbar;

6. heating from-25 ℃ to 0 ℃, wherein the heating rate is about 0.5-1 ℃/min, the temperature is maintained for 8-12 hours, and the vacuum degree is 0.01 mbar;

7. finally, heating from 0 ℃ to 25 ℃, wherein the heating rate is about 0.5-1 ℃/min, the temperature is maintained for 6-10 hours, and the vacuum degree is 0.1 mbar; after freeze-drying, obtaining the freeze-dried powder mask;

(2) preparing essence solution solvent:

1. at room temperature, adding deionized water into a vacuum homogenizing emulsifying pot, heating to 85-90 ℃, preserving heat for 30 minutes for sterilization, then cooling to below 60 ℃, sequentially adding poly gamma-sodium glutamate, glycerol, 1, 2-pentanediol and glycerol glucoside, and stirring to dissolve uniformly; sequentially adding betaine and dipotassium glycyrrhizinate, and stirring uniformly;

2. then cooling to 50 ℃, sequentially adding the honey extract, the yeast β -glucan and the nicotinamide, stirring, mixing and dissolving uniformly;

3. cooling to 40 deg.C, sequentially adding carob seed fruit extract, herba Centellae root extract, Scutellariae radix root extract, and chelating agent, stirring, mixing, and dissolving to obtain essence solution.

In a preferred embodiment, the preparation method of the freeze-dried powder live mask of the plant protein and polypeptide formula comprises the following steps:

(1) preparing a freeze-dried powder mask:

1. adding deionized water into a vacuum homogenizing emulsifying pot at room temperature, heating to 88 deg.C, maintaining the temperature for 30 min for sterilization, cooling to 80 deg.C, sequentially adding mannitol, pullulan and sodium hyaluronate, stirring and dissolving uniformly;

4. putting the freeze-drying plate into a vacuum freeze-drying machine, starting the freeze-drying machine, setting freeze-drying process parameters, freezing the mask cloth from room temperature to-50 ℃, and maintaining for 3.5 hours to fully freeze the freeze-drying liquid on the mask cloth;

5. gradually heating the frozen and compacted mask cloth from-50 ℃ to-25 ℃, wherein the heating rate is 0.5 ℃/min, the vacuum degree is 0.01mbar and the temperature is maintained for 14 hours;

6. then heating from-25 ℃ to 0 ℃, wherein the heating rate is about 0.5 ℃/minute, the temperature is maintained for 10 hours, and the vacuum degree is 0.01 mbar;

7. finally, heating from 0 ℃ to 25 ℃, wherein the heating rate is about 0.8 ℃/minute, the temperature is maintained for 8 hours, and the vacuum degree is 0.1 mbar; after freeze-drying, obtaining the freeze-dried powder mask;

(2) preparing essence solution solvent:

1. at room temperature, adding deionized water into a vacuum homogenizing emulsifying pot, heating to 88 ℃, preserving heat for 30 minutes for sterilization, then cooling to below 60 ℃, sequentially adding poly gamma-sodium glutamate, glycerol, 1, 2-pentanediol and glycerol glucoside, and stirring for dissolving uniformly; sequentially adding betaine and dipotassium glycyrrhizinate, and stirring uniformly;

The third aspect of the invention provides a use method of a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which comprises the following steps: after the face and the hands are thoroughly cleaned, pouring the essence solvent into the freeze-dried powder mask, completely soaking the freeze-dried powder mask by the essence solvent, taking out the freeze-dried powder mask, spreading, applying the freeze-dried powder mask on the face for 20-25 minutes, taking down, and slightly beating until the essence is completely absorbed.

Examples

Hereinafter, the present invention will be described in more detail by way of examples, but it should be understood that these examples are merely illustrative and not restrictive. The starting materials used in the examples which follow are all commercially available unless otherwise stated.

Example 1

The embodiment 1 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which comprises freeze-dried powder, mask cloth and an essence solvent; the facial mask cloth is a cotton film cloth.

The freeze-dried powder comprises, by weight, 5 parts of hydrolyzed plant protein, 3.55 parts of an excipient, 0.2 part of polypeptide and 100 parts of deionized water.

The excipient comprises 2.5 parts of mannitol, 1 part of pullulan and 0.05 part of sodium hyaluronate in parts by weight.

The polypeptide comprises 0.1 part of carnosine, 0.03 part of oligopeptide-1, 0.02 part of acetyl hexapeptide-8 and 0.05 part of palmitoyl pentapeptide-4 in parts by weight.

The hydrolyzed vegetable protein is hydrolyzed soybean protein; the molecular weight of the sodium hyaluronate is 80-130 kilodaltons.

The essence solvent comprises 8.03 parts by weight of skin conditioner, 2.5 parts by weight of humectant, 0.1 part by weight of anti-inflammatory agent, 0.1 part by weight of chelating agent and 100 parts by weight of deionized water; the anti-inflammatory agent is dipotassium glycyrrhizinate.

The skin conditioner comprises, by weight, 0.03 parts of poly gamma-sodium glutamate, 1 part of glycerol glucoside, 1 part of yeast- β glucan, 1 part of betaine, 1 part of nicotinamide and 4 parts of plant extract.

The plant extract comprises 1 part of carob seed fruit extract, 1 part of honey extract, 1 part of asiatic centella root extract and 1 part of baical skullcap root extract in parts by weight.

The humectant comprises 2 parts by weight of glycerol and 0.5 part by weight of 1, 2-pentanediol.

The chelating agent comprises 0.005 parts by weight of caprylyl hydroximic acid, 0.03 parts by weight of hexanediol and 0.065 parts by weight of 1, 3-propanediol.

The preparation method of the freeze-dried powder live mask with the vegetable protein and polypeptide formula comprises the following steps:

(1) preparing a freeze-dried powder mask:

(2) preparing essence solution solvent:

Pouring the essence solvent into the lyophilized powder facial mask to make the lyophilized powder facial mask completely soaked by the essence solvent, and taking out the lyophilized powder facial mask to obtain the directly-used facial mask.

Example 2

The embodiment 2 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which comprises freeze-dried powder, mask cloth and an essence solvent; the mask cloth is cotton cloth mask cloth.

The freeze-dried powder comprises, by weight, 8 parts of hydrolyzed plant protein, 11.2 parts of an excipient, 1.3 parts of polypeptide and 100 parts of deionized water.

The excipient comprises 6 parts of mannitol, 5 parts of pullulan and 0.2 part of sodium hyaluronate according to parts by weight.

The polypeptide comprises 0.3 part of carnosine, 0.5 part of oligopeptide-1, 0.3 part of acetyl hexapeptide-8 and 0.2 part of palmitoyl pentapeptide-4 in parts by weight.

The essence solvent comprises 40.1 parts of skin conditioner, 7 parts of humectant, 0.3 part of anti-inflammatory agent, 1 part of chelating agent and deionized water by weight part to 100 parts; the anti-inflammatory agent is dipotassium glycyrrhizinate.

The skin conditioner comprises, by weight, 0.1 part of poly gamma-sodium glutamate, 5 parts of glycerol glucoside, 5 parts of yeast- β glucan, 5 parts of betaine, 5 parts of nicotinamide and 20 parts of plant extract.

The plant extract comprises, by weight, 5 parts of carob seed fruit extract, 5 parts of honey extract, 5 parts of asiatic centella root extract, 5 parts of baical skullcap root extract.

The humectant comprises 5 parts of glycerol and 2 parts of 1, 2-pentanediol according to parts by weight.

The chelating agent comprises 0.05 parts by weight of caprylhydroxamic acid, 0.3 parts by weight of hexanediol and 0.65 parts by weight of 1, 3-propanediol.

The specific implementation mode of the preparation method of the freeze-dried powder live mask with the vegetable protein and polypeptide formula is the same as that in example 1.

Example 3

The embodiment 3 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which comprises freeze-dried powder, mask cloth and an essence solvent; the mask cloth is cotton cloth mask cloth.

The freeze-dried powder comprises, by weight, 7 parts of hydrolyzed plant protein, 4.6 parts of excipient, 0.72 part of polypeptide and 100 parts of deionized water.

The excipient comprises 3 parts of mannitol, 1.5 parts of pullulan and 0.1 part of sodium hyaluronate according to parts by weight.

The polypeptide comprises 0.2 part of carnosine, 0.3 part of oligopeptide-1, 0.15 part of acetyl hexapeptide-8 and 0.07 part of palmitoyl pentapeptide-4 in parts by weight.

The essence solvent comprises 15.54 parts of skin conditioner, 4.2 parts of humectant, 0.2 part of anti-inflammatory agent, 0.8 part of chelating agent and deionized water which are supplemented to 100 parts; the anti-inflammatory agent is dipotassium glycyrrhizinate.

The humectant comprises 3 parts of glycerol and 1.2 parts of 1, 2-pentanediol according to parts by weight.

The skin conditioner comprises, by weight, 0.04 parts of poly gamma-sodium glutamate, 1.5 parts of glycerol glucoside, 2 parts of yeast- β glucan, 2 parts of betaine, 2 parts of nicotinamide and 8 parts of plant extract.

The plant extract comprises 2 parts of carob seed fruit extract, 2 parts of honey extract, 2 parts of asiatic centella root extract and 2 parts of baical skullcap root extract by weight.

The chelating agent comprises 0.04 parts by weight of caprylhydroxamic acid, 0.24 parts by weight of hexanediol and 0.52 parts by weight of 1, 3-propanediol.

Example 4

The embodiment 4 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, and the specific implementation mode is the same as that in the embodiment 3, except that the pullulan is replaced by trehalose.

The specific implementation mode of the preparation method of the freeze-dried powder live facial mask with the vegetable protein and polypeptide formula is the same as that in example 1, except that the pullulan is replaced by trehalose.

Example 5

The embodiment 5 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which is similar to the embodiment 3 in the specific implementation mode, and is different from the embodiment 3 in that the molecular weight of the sodium hyaluronate is 60 ten thousand daltons and is purchased from Shandong Huanfurtanda biomedicine Limited company.

Example 6

The embodiment 6 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which is similar to the embodiment 3 in the specific implementation mode, and is different from the embodiment 3 in that the molecular weight of the sodium hyaluronate is 150 kilodaltons and is purchased from Shandong Hua XifuRuida biological medicine limited company.

Example 7

The embodiment 7 of the invention provides a freeze-dried powder live mask with a formula of plant proteins and polypeptides, which is the same as that of embodiment 3 in specific implementation mode, and is different from that of embodiment 3 in that the skin conditioner comprises 1.5 parts of glycerol glucoside, 2 parts of yeast- β glucan, 2 parts of betaine, 2 parts of nicotinamide and 8 parts of plant extracts in parts by weight, and the plant extracts comprise 2 parts of honey extract, 2 parts of asiatic centella root extract and 2 parts of scutellaria root extract.

Example 8

The embodiment 8 of the invention provides a freeze-dried powder live mask with a formula of plant proteins and polypeptides, which is the same as that of embodiment 3 in specific implementation mode, and is different from that of embodiment 3 in that the skin conditioner comprises 1.5 parts of glycerol glucoside, 2 parts of yeast- β glucan, 2 parts of betaine, 2 parts of nicotinamide and 8 parts of plant extracts in parts by weight, and the plant extracts comprise 2 parts of carob bean fruit extract, 2 parts of honey extract, 2 parts of asiatic centella root extract and 2 parts of scutellaria root extract.

Example 9

The embodiment 9 of the invention provides a freeze-dried powder live mask with a formula of plant proteins and polypeptides, which is the same as that of embodiment 3 in specific implementation mode, and is different from that of embodiment 3 in that the skin conditioner comprises, by weight, 0.04 part of poly gamma-sodium glutamate, 1.5 parts of glycerol glucoside, 2 parts of yeast- β glucan, 2 parts of betaine, 2 parts of nicotinamide and 8 parts of plant extracts, wherein the plant extracts comprise 2 parts of honey extract, 2 parts of asiatic centella root extract and 2 parts of scutellaria root extract.

Example 10

The embodiment 10 of the invention provides a freeze-dried powder live mask with a vegetable protein and polypeptide formula, which is similar to the embodiment 3 in the specific implementation manner, and is characterized in that the freeze-dried powder comprises 15.54 parts by weight of a skin conditioner, and the part by weight of the skin conditioner in an essence solvent is 0.

(1) preparing a freeze-dried powder mask:

2. cooling to below 30 deg.C under stirring, sequentially adding carnosine, oligopeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-4, hydrolyzed vegetable protein, and skin conditioner, stirring for 10 min, mixing completely and dissolving, filtering, and discharging to obtain lyophilized solution;

(2) preparing essence solution solvent:

1. at room temperature, adding deionized water into a vacuum homogenizing emulsifying pot, heating to 88 ℃, preserving heat for 30 minutes for sterilization, then cooling to below 60 ℃, sequentially adding poly gamma-sodium glutamate, glycerol, 1, 2-pentanediol and glycerol glucoside, and stirring for dissolving uniformly; sequentially adding betaine and dipotassium glycyrrhizinate, and stirring;

2. cooling to 50 deg.C, sequentially adding Mel extract, yeast β -dextran, and nicotinamide, stirring, mixing, and dissolving;

Performance evaluation:

the following performance evaluations were made using example 3 as a template.

1. Sensory and physicochemical indexes: the test results are shown in Table 1.

TABLE 1 sensory, physicochemical indices

Remarking: receiving a test sample: and (3) completely soaking and dissolving the freeze-dried powder mask by 25ml of essence solvent, and extruding out a dissolving solution, namely the mask essence after the essence solvent and the freeze-dried powder mask are mixed and dissolved.

2. The hygiene indexes are as follows: the test results are shown in Table 2.

TABLE 2 hygiene index

Remarking: 1) receiving a test sample: and (3) completely soaking and dissolving the freeze-dried powder mask by 25ml of essence solvent, and extruding out a dissolving solution, namely the mask essence after the essence solvent and the freeze-dried powder mask are mixed and dissolved.

2) The detection method comprises the following steps: inspection method for microbial limit in appendix XI J of second part of Chinese pharmacopoeia 2010 edition

3. Toxic substance index: the test results are shown in Table 3.

TABLE 3 toxic substance index

2) The detection method comprises the following steps: cosmetic safety specifications (2015 edition).

4. Multiple skin irritation tests: the test results are shown in Table 4.

Table 4 multiple skin irritation tests

Remarking: 1) receiving a test sample: the facial mask is prepared by completely soaking essence solution solvent and lyophilized powder facial mask. 2) The test method comprises the following steps: the test animal is rabbit. The test specimen was applied to one side of the spine of an experimental animal with the hair removed range of 3cmx 3cm and the application area of 2.5cmx 2.5cm, 1 time per day as a control, and continuously applied for 14 days, before the test according to the cosmetic safety specification (2015 edition). Starting on day 2, the remaining test substance was removed by shearing before each use, the results were observed after 1 hour, scored according to table 1 in the specification, the mean integral was calculated according to the formula, and the stimulation intensity was graded according to table 2 in the specification.

5. Eye irritation test: the test results are shown in Table 5.

Table 5 eye irritation test

Remarking: 1) receiving a test sample: and (3) completely soaking and dissolving the freeze-dried powder mask by 25ml of essence solvent, and extruding out a dissolving solution, namely the mask essence after the essence solvent and the freeze-dried powder mask are mixed and dissolved. 2) The test method comprises the following steps: the test sample is dropped into the conjunctival sac of one eye of the rabbit, the eye is closed for one second, and the other eye is not treated to be used as self control. Observation and rating were required after dropping the test substance according to the cosmetic safety specifications (2015 edition).

6. And (4) testing the moisturizing effect:

1) application example 3 the moisturizing effect 8 hours after 1 time of the facial mask (test sample, the same below) in which the essence solution solvent and the freeze-dried powder facial mask were soaked and dissolved is shown in fig. 1.

The test method comprises the following steps: the test specimens were applied once in the morning to 16 dry skin volunteers, and the skin conductivity was measured with a skin moisture tester (SKICON-200EX-USB) at random 8 hours after marking the skin area.

The results in fig. 1 show that the test sample can be durably moisturized for 8 hours by only one application, and the difference is significant compared with the control (P < ═ 0.001).

2) The moisturizing effect of the test sample obtained in example 3 was continuously applied for 5 days and was stopped for 3 days after one day as shown in fig. 2.

The test method comprises the following steps: 14 volunteers were applied once in the morning for 5 consecutive days. Skin conductivity was measured with a skin moisture tester (SKICON-200EX-USB) 8 hours daily after the marked skin area. The following 3 days of the regression period were no treatment.

The results in fig. 2 show that skin moisturization was significantly increased by 5 days of continuous application. After 3 days of rest, the moisturizing effect was still significantly higher than the control (water film). Indicating that the subject can increase skin hydration and tend to healthier skin conditions.

7. Skin barrier damage repair effect test: see fig. 3.

Test method tape-tearing model method five volunteers, namely, induced erythema on the inner side of the arm by tape-tearing (skin stimulation causes change of percutaneous water loss TEW L), and then treated by applying test substances (facial mask obtained by soaking and dissolving essence solution solvent and freeze-dried powder facial mask obtained in example 3) and placebo (water) control samples on selected areas respectively, and detecting the change value of TEW L (△ TEW L value).

Fig. 3 shows that the TEW L of the skin after being torn off the tape first rises significantly, and gradually decreases with time to the TEW L, but the drop amplitude is the largest after the facial mask dissolved by the essence solvent and the freeze-dried powder facial mask in example 3 is applied, and the facial mask has obvious statistical difference (TEW L transdermal moisture tester) compared with blank control and placebo, which indicates that the facial mask dissolved by the essence solvent and the freeze-dried powder facial mask in example 3 accelerates the repair of the damaged skin barrier by providing an exogenous lipid barrier, and can provide protection for sensitive skin.

8. Skin improvement effect test:

1) skin elasticity test, see fig. 4.

The test method comprises the following steps: selecting 12 female healthy volunteers, and applying the test substance (facial mask obtained by soaking and dissolving essence solution solvent and lyophilized powder facial mask obtained in example 3) and the control sample (water film) on the left and right half faces respectively once a day; double-blind, random testing; detection indexes are as follows: skin elasticity (skin elasticity tester custom metal MPA 580).

Fig. 4 shows that the facial mask soaked and dissolved by the essence solvent and the freeze-dried powder facial mask obtained in example 3 increases the skin elasticity by 10% on average, and shows that the facial mask has a good anti-aging effect.

2) Facial fine line test, see fig. 5.

The test method comprises the following steps: selecting 12 female healthy volunteers, and using the test substance (facial mask obtained by soaking and dissolving essence solution solvent and freeze-dried powder facial mask obtained in example 3) and the control sample (water) on the left and right half faces respectively once a day; double-blind, random testing; detection indexes are as follows: facial striae (VISA full face shot, striae area calculation in the corner of the eye and cheek regions).

The results in fig. 5 show that the area of fine wrinkles is reduced by 9% on average after using the mask obtained in example 3, showing a better anti-wrinkle effect.

9. And (3) uniformity testing: recording whether the freeze-dried powder in the freeze-dried powder mask obtained in the embodiment 1-10 is uniformly dispersed and does not have agglomeration, if the freeze-dried powder is uniformly dispersed and does not have agglomeration, recording that the freeze-dried powder in the mobile freeze-dried powder mask does not fall off and recording as qualified, and otherwise recording as unqualified. The results are shown in Table 6.

TABLE 6

Examples	Uniformity test
		Example 1	Qualified
Example 2	Qualified
		Example 3	Qualified
Example 4	Fail to be qualified
		Example 5	Fail to be qualified
Example 6	Fail to be qualified
		Example 7	Qualified
Example 8	Qualified
		Example 9	Qualified
Example 10	Qualified

From the performance evaluation results, the sensory, physicochemical and hygienic indexes of the freeze-dried powder live mask provided by the invention all meet the relevant product standards, and the freeze-dried powder live mask has good moisturizing, barrier repairing, anti-wrinkle and anti-aging effects, is safe and has no stimulation.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. A freeze-dried powder live facial mask with a vegetable protein and polypeptide formula is characterized by comprising freeze-dried powder, facial mask cloth and an essence solvent; the freeze-dried powder comprises the following raw materials, by weight, 5-8 parts of hydrolyzed plant protein, 3-15 parts of an excipient, 0.2-1.3 parts of polypeptide and 100 parts of deionized water; the excipient comprises, by weight, 2.5-6 parts of mannitol, 1-5 parts of pullulan and 0.05-0.2 part of sodium hyaluronate.

2. The lyophilized powder live mask of a plant protein and polypeptide formula according to claim 1, wherein the molecular weight of the sodium hyaluronate is 80-130 ten thousand daltons.

3. The lyophilized powder live mask of a plant protein and polypeptide formula according to claim 1, wherein the polypeptide comprises, by weight, 0.1-0.3 parts of carnosine, 0.03-0.5 parts of oligopeptide-1, 0.01-0.3 parts of acetyl hexapeptide-8, and 0.05-0.2 parts of palmitoyl pentapeptide-4.

4. The lyophilized powder live mask according to claim 1, wherein the essence solvent comprises, by weight, 8-41 parts of a skin conditioner, 2.5-7 parts of a humectant, 0.1-0.3 part of an anti-inflammatory agent, 0.1-1 part of a chelating agent, and 100 parts of deionized water.

5. The lyophilized powder live mask of claim 4, wherein the skin conditioner comprises, by weight, 0.03-0.1 parts of poly-gamma-sodium glutamate, 1-5 parts of glycerol glucoside, 1-5 parts of yeast- β glucan, 1-5 parts of betaine, 1-5 parts of nicotinamide, and 4-20 parts of plant extract.

6. Lyophilized powder live mask of a plant protein and polypeptide formulation according to claim 5, wherein the plant extract comprises carob seed fruit extract, honey extract, centella asiatica root extract, scutellaria baicalensis root extract.

7. A freeze-dried powder live mask of a plant protein and polypeptide formula according to claim 6, wherein the plant extract comprises 1-5 parts by weight of carob bean fruit extract, 1-5 parts by weight of honey extract, 1-5 parts by weight of centella asiatica root extract, and 1-5 parts by weight of scutellaria baicalensis root extract.

8. The lyophilized powder and live mask of a plant protein and polypeptide formulation according to any one of claims 4 to 7, wherein the chelating agent comprises caprylhydroxamic acid and alcohol by weight.

9. The lyophilized powder and live mask of a plant protein and polypeptide formulation according to any one of claims 1 to 7, wherein the hydrolyzed plant protein comprises at least one of hydrolyzed soy protein, hydrolyzed corn protein, and hydrolyzed wheat protein.

10. A method for preparing a freeze-dried powder live mask with a plant protein and polypeptide formula according to any one of claims 4 to 9, which is characterized by comprising the following steps:

(1) the preparation of the freeze-dried powder mask comprises the steps of keeping the temperature of deionized water for sterilization, adding an excipient at 80 ℃, stirring for dissolving uniformly, adding polypeptide and hydrolyzed plant protein at 30 ℃, stirring for mixing for dissolving uniformly, filtering to obtain freeze-dried liquid, soaking the freeze-dried liquid into a mask cloth with the amount of 10-15 m L/piece for saturation and no liquid dripping, filling the mask cloth into a freeze-drying tray, and freeze-drying in a vacuum freeze-dryer to obtain the freeze-dried powder mask;