CN108670901B - Skin softening lotion with macadamia nut protein peptide and green tangerine peel extract as active ingredients and preparation method thereof - Google Patents
Skin softening lotion with macadamia nut protein peptide and green tangerine peel extract as active ingredients and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a smoothing toner taking macadamia nut protein peptide and green tangerine peel extract as active ingredients, which is prepared from the following raw and auxiliary materials in percentage by weight: macadamia nut protein peptide 2.0% -6.0%; 2.0 to 4.0 percent of extract of green tangerine orange peel of macadamia nut; 2.0% -5.0% of glycerin; 3.0 to 6.0 percent of propylene glycol; 2.0 to 3.5 percent of trehalose; 0.5-1.5% of allantoin; hyaluronic acid 2.0-4.0%; 0.2 to 0.5 percent of xanthan gum; 0.1 to 0.2 percent of methyl hydroxybenzoate; 0.1 to 0.2 percent of phenoxyethanol; 0.1 to 0.3 percent of ethylhexyl glycerol; PEG-40 hydrogenated castor oil 0.4-0.6%; 0.01 to 0.03 percent of essence; the balance being water. The skin softening lotion which takes the macadimia nut protein peptide and the green tangerine orange peel extracting solution as active ingredients has the advantages that the macadimia nut protein peptide and the macadimia nut green tangerine orange peel extracting solution have the synergistic stable structure and the capability of eliminating free radicals, can play good effects of moisturizing, resisting wrinkles and inhibiting and preventing skin aging on facial skin, and does not irritate the facial skin.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to moisturizing and anti-wrinkle smoothing toner containing macadimia nut active ingredients and a preparation method thereof.
Background
With the improvement of living standard of people, the requirements of people on cosmetics are increasingly improved, and particularly skin care products are directly related to the health and beauty of skin. The smoothing toner in the skin care product is a toning lotion with the pH value close to 7, is suitable for dry skin and mixed skin, and is used for beautifying and protecting the skin after the face cleanser is applied and before the face cleanser is applied. The existing smoothing toner is composed of a humectant, a moisturizing grease, a surfactant, a skin conditioner, other functional auxiliaries and the like, for example, a patent (CN201110227336.8) discloses a smoothing toner which is specially used for facial skin of a person and has whitening and moisturizing effects, and the smoothing toner is composed of: 1. 3 butanediol, sodium pyrrolidone carboxylate, dipotassium glycyrrhizinate, NMF-50, antiallergic agent (CD-2901), purified water, hyaluronic acid (1%), arbutin, azelaic acid derivatives and other raw materials are heated, kept warm and stirred to prepare the compound. Although it has a certain whitening effect, it has poor antioxidant and anti-wrinkle effects. In addition, most of the Chinese herbal medicine active ingredients are used as the components of the smoothing toner, for example, a patent (CN201510675132.9) discloses an angelica sinensis rhodiola rosea smoothing toner and a preparation method thereof, the smoothing toner usually contains macromolecular protein, is easy to cause allergy, and the taste of the traditional Chinese medicine is not easy to accept.
Currently, Macadamia ternifolia (Macadamia ternifolia f. muell.) is also called Macadamia walnuts, Macadamia nuts and the like, and belongs to the genus Macadamia of the dragon eye. Native to subtropical rainforests of queensland and new south welsh, australia. The nuts of the fruit tree have unique flavor and rich nutrient components, are considered to be one of the best table nuts in the world overseas and have the reputation of "dried fruit queen"; has been developed into one of the four famous nuts in the world.
The macadimia nut has rich nutrition value, the protein content is more than 9%, 8 amino acids which are necessary for human bodies are contained, and the macadimia nut is hot-bated, and particularly, the protein peptide with various biological activities can be prepared from the macadimia nut protein. Protein peptides are a general term for various peptides ranging from dipeptides in which amino acids are composed in various compositions and arrangements to complex linear, cyclic structures, and are multifunctional compounds derived from proteins, and many protein peptides also have new functions that the original proteins or their constituent amino acids do not have. Macadimia nut protein peptide has stronger biological activities of oxidation resistance, blood pressure reduction, bacteria resistance and the like, has good solubility in a wider pH range, has higher hygroscopicity and moisture retention, has the advantages of definite action target, high safety, small side effect, obvious effect and the like by adding a small amount of protein peptide according to the research report of related peptide skin care products in the industry, is more and more popular in various cosmetics, but has poorer stability, is easy to be inactivated by being influenced by various enzymes or external factors, so that the storage cost is higher, and the price of raw materials is relatively expensive.
Although the outermost green tangerine peel of macadimia nuts accounts for 45-60% of the fresh weight of the whole fruit, the outermost green tangerine peel of macadimia nuts is only used as a retting fertilizer and a feed in a very small amount, most of the green tangerine peel is not developed and utilized and is usually discarded as waste, and the outermost green tangerine peel can be applied to the fields of cosmetics, health-care foods and the like due to the fact that the outermost green tangerine peel contains arbutin, helicidum, catechol, gallic acid, polyphenol, polysaccharide and other components. There are related reports that effective components are extracted from macadimia nut green peel and applied, and a patent (CN201611140423.9) discloses a macadimia nut green peel extracting solution, a cosmetic taking the macadimia nut green peel extracting solution as an active component and a preparation method of the cosmetic, but the content of a single component capable of playing a skin care role in the macadimia nut green peel extracting solution is extremely low, and the macadimia nut green peel extracting solution is not widely popularized and used.
In conclusion, the inventor researches a smoothing toner taking macadamia nut protein peptide and green tangerine peel extract as active ingredients and a preparation method thereof for many years, and solves the problems that a single protein peptide solution is poor in stability, particularly thermal/optical variability is easy to occur, no synergistic effect exists between the protein peptide and chemical components such as non-protein substances, and the solubility, the moisture retention and the anti-aging effect are poor.
Disclosure of Invention
In view of the above, the invention provides a preparation method of a smoothing toner using macadamia nut protein peptide and green tangerine peel extract as active ingredients, which solves the problems in the prior art.
On one hand, the invention provides a smoothing toner taking macadamia nut protein peptide and green tangerine peel extract as active ingredients, which is prepared from the following raw and auxiliary materials in percentage by weight:
the composite moisturizing factor is composed of glycerol, propylene glycol, trehalose, allantoin, hyaluronic acid and xanthan gum, and can enhance the moisturizing capability of the smoothing toner and adjust the acid-base balance of a solution system. Methyl hydroxybenzoate and phenoxyethanol play a role in antisepsis and bacteriostasis, and ethylhexyl glycerin and PEG-40 hydrogenated castor oil play a role in surfactant, which is helpful for better dissolving protein peptide and polyphenol compounds in water; the sage essential oil has the function of increasing fragrance.
The smoothing toner prepared from the following raw and auxiliary materials in percentage by weight is preferably selected through experiments, and the moisturizing and anti-aging capacity of the smoothing toner is more remarkable:
in particular, the macadamia nut protein peptide is prepared by the following steps:
step S101: weighing 5.000g of macadimia nut protein powder, dissolving in 100mL of ultrapure water, adjusting the pH of the solution to 10.0 by using 1.0mol/L sodium hydroxide, adding protease accounting for 5.0% of the weight of the macadimia nut protein powder, and uniformly mixing; the protease comprises alkaline protease and compound protease with the mass ratio of 3: 1;
step S102: adopting dynamic high-pressure microjet technology to assist enzymolysis of the solution in the step S101, wherein the treatment pressure is 50MPa, the treatment time is 60min, after the enzymolysis is finished, carrying out enzyme deactivation treatment for 15min in water bath at 80 ℃, cooling, and then adjusting the pH of the enzymolysis solution to 7.0 by using 1.0mol/L hydrochloric acid;
step S103: and (4) centrifuging the step S102 by using a disk centrifuge, performing ultrafiltration treatment on the obtained clear solution by using a 3kDa ultrafiltration membrane, controlling the ultrafiltration flow rate to be 2.0ml/min, pressurizing by using nitrogen, and concentrating the ultrafiltration solution with the molecular weight of less than 3kDa to be 0.2 times of the original solution by using reduced pressure distillation to obtain the concentrated solution, namely the macadamia nut protein peptide. The protein peptide prepared by the method at least contains 16 amino acids, particularly has the highest content of glutamic acid and arginine, and various amino acids cooperate with each other to comprehensively maintain the skin. The average particle size of the solution is 1.1um, the particle size is small, the distribution is narrow, and the absorption by skin is facilitated; small molecular weight and strong oxidation resistance.
The macadimia nut green husk extracting solution is prepared by the following steps:
step S104: vacuum freeze drying fresh green peel of macadimia nut for 16-20h until the water content is less than 3.0%, and micronizing to obtain particles smaller than 25 μm;
step S105: taking 100g of the macadimia nut green husk powder, adding a 60% ethanol solution according to the weight ratio of 1:20, treating for 5min at 40MPa by using a dynamic high-pressure micro-jet homogenizer, then leaching for 80min in a water bath at 55 ℃, and then clarifying by using a disk centrifuge, wherein the clarified liquid is the initial liquid of the macadimia nut green husk extraction;
step S106: and (3) concentrating the initial liquid of the macadimia nut green husk extract by reduced pressure distillation to 0.1 time of the volume of the initial liquid to obtain the macadimia nut green husk extract. The content of polyphenol compounds in the macadimia nut green husk extracting solution extracted by the method reaches 87.6 percent.
In addition, the preparation method of the smoothing toner taking the macadimia nut protein peptide and the green tangerine peel extracting solution as active ingredients is also provided, and is characterized by comprising the following steps: the method comprises the following steps:
step S107: taking trehalose, allantoin, hyaluronic acid and xanthan gum according to the mass ratio of the raw materials and the auxiliary materials, adding a certain amount of purified water into a steam heating interlayer tank, heating to 80 ℃, preserving heat, stirring and dissolving uniformly to obtain a first mixed solution;
step S108: according to the mass ratio of the raw materials to the auxiliary materials, putting glycerol, propylene glycol, methyl hydroxybenzoate, phenoxyethanol, ethylhexyl glycerol and PEG-40 hydrogenated castor oil into another steam heating interlayer tank, heating to 80 ℃, and completely dissolving and uniformly mixing to obtain a second mixed solution;
step S109: slowly adding the second mixed solution obtained in the step S108 into the first mixed solution obtained in the step S107 through a sterile pipeline, carrying out heat preservation treatment for 10min, cooling to 35 ℃, sequentially adding macadimia nut protein peptide, macadimia nut green tangerine peel extracting solution and sage essential oil according to the mass ratio of the raw materials and the auxiliary materials, uniformly stirring, and carrying out clarification treatment through a disk centrifuge to obtain the smoothing toner taking the macadimia nut protein peptide and the green tangerine peel extracting solution as active ingredients.
The skin softening lotion which takes the macadimia nut protein peptide and the green tangerine orange peel extracting solution as active ingredients has the advantages that the macadimia nut protein peptide and the macadimia nut green tangerine orange peel extracting solution have the synergistic structure stability and the free radical eliminating capability, can play a good role in moisturizing, resisting wrinkles and inhibiting and preventing skin aging on facial skin, does not irritate the facial skin, and is simple in preparation method process, low in investment cost and suitable for mass production.
Furthermore, the macadimia nut protein peptide and the macadimia nut green husk extracting solution are prepared by an independent research and development process, and the same patent application protection is carried out, the macadimia nut protein peptide and the macadimia nut green husk extracting solution prepared by the process are main active ingredients for moisturizing, anti-wrinkle and allergy-relieving, and particularly the turbid state easily appearing due to poor solubility of the protein peptide and the macadimia nut green husk extracting solution is solved.
Furthermore, the ratio of the components of the smoothing toner improves the stability of a smoothing toner system, promotes skin absorption, synergistically enhances the free radical eliminating capacity, and has the capacity of eliminating DPPH free radicals as high as 92% and ABTS free radicals as high as 88%. The pH value of the smoothing toner prepared by the method is 6.8, the smoothing toner is clear, has no obvious impurities or black spots, is colorless, does not layer, has no oil-water separation phenomenon after centrifugation at 4000r/min, and meets the quality standard of the smoothing toner.
Drawings
FIG. 1 is a schematic flow chart of a method for preparing a smoothing toner containing macadamia nut protein peptide and green tangerine peel extract as active ingredients according to the present invention;
fig. 2 is a graph of laser scattering particle size distribution of macadamia nut protein peptides.
Detailed Description
The principles and features of this invention are described below in conjunction with examples which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
The ultrahigh pressure micro-jet homogenizer adopted by the embodiment of the invention has the following model: m-100EH-30, manufacturer: microfluidics, usa; continuous dish formula centrifugation unit, model: SE03.0V, manufacturer: sptoler, Italy. The commercially available smoothing toner is a smoothing toner of a certain brand, and comprises the components of water, butanediol, glycerol, cyclopenta dimethyl siloxane, isopropyl palmitate, PEG-60 hydrogenated castor oil, honey, mannitol, tocopheryl acetate, sweet mandelic protein, sodium hyaluronate and yeast extract; the macadimia nut protein powder is provided by a macadimia nut production base of south Asia tropical crop research institute of Chinese tropical agricultural academy of sciences, and is extracted by the existing alkali extraction and acid precipitation method.
The first embodiment is as follows: preparation of macadamia nut protein peptide
The preparation method of the macadimia nut protein peptide comprises the following steps:
s101: weighing 5.000g of macadimia nut protein powder, dissolving the macadimia nut protein powder in 100mL of ultrapure water, adjusting the pH of the solution to 10.0 by using 1.0mol/L sodium hydroxide, adding alkaline protease (0.1875g) and compound protease (0.0625g) which are 5.0% of the weight of the macadimia nut protein powder (the mass ratio of the alkaline protease to the compound protease is 3:1), and uniformly mixing;
s102: carrying out enzymolysis on the solution obtained in the step S101 by using a dynamic high-pressure microjet homogenizer under 50MPa for 60min by using a dynamic high-pressure microjet technology for assisting enzymolysis, carrying out enzyme deactivation treatment for 15min in a water bath at 80 ℃ after the enzymolysis is finished, and adjusting the pH of an enzymolysis solution to 7.0 by using 1.0mol/L hydrochloric acid after cooling;
s103: and (2) centrifuging the enzymatic hydrolysate obtained in the step (S102) by using a disk centrifuge, performing ultrafiltration treatment on the obtained clear solution by using a 3kDa ultrafiltration membrane, controlling the ultrafiltration flow rate to be 2.0mL/min, pressurizing by using nitrogen, concentrating the ultrafiltration solution with the molecular weight of less than 3kDa to be 0.2 times of the original solution by using reduced pressure distillation, and determining the obtained concentrated solution to be the macadimia nut protein peptide (20mL), wherein the macadimia nut protein peptide prepared by the embodiment at least contains 16 amino acids, especially the content of glutamic acid and arginine is highest, and various amino acids cooperate with one another to comprehensively maintain the skin. As shown in table 1, the average particle size of the macadamia nut protein peptide solution was 1.1um as measured by DLS, and the solution had a small particle size and narrow distribution, which contributed to skin absorption. As shown in fig. two.
Table 1 example one preparation of amino acid composition of macadamia nut protein peptide
Example two: preparation of macadimia nut green husk extracting solution
The preparation method of the macadimia nut green husk extracting solution comprises the following steps:
s104: vacuum freeze drying fresh macadimia nut green peel for 16h until the water content is 2.5%, and then pulverizing the dried macadimia nut green peel into material particles smaller than 25um by adopting an ultramicro pulverization technology for later use;
s105: taking 100g of the macadimia nut green husk powder prepared in the step S104, adding 2000g of 60% ethanol solution according to the mass ratio of 1:20, uniformly mixing, processing for 5min under 40MPa by using a dynamic high-pressure micro-jet homogenizer, leaching for 80min in water bath at 55 ℃, and finally clarifying by using a disc centrifuge to obtain an initial macadimia nut green husk extraction liquid;
s106: and (3) concentrating the initial liquid of the extract of the green tangerine orange peel of the macadimia nut prepared in the step (S105) to 0.1 time of the volume of the initial liquid through reduced pressure distillation (at the temperature of 35 ℃ and under the pressure of 20Pa), namely 20mL of extract of the green tangerine orange peel of the macadimia nut is obtained, and the content of polyphenol compounds in the extract of the green tangerine orange peel of the macadimia nut is determined to be 87.6%.
Example three: a preparation method of smoothing toner with macadamia nut protein peptide and green tangerine peel extract as active ingredients comprises the following steps:
s107: taking 3.0g of trehalose, 1.0g of allantoin, 3.0g of hyaluronic acid and 0.3g of xanthan gum according to the mass ratio of raw materials and auxiliary materials in a first steam heating interlayer tank, adding 80.735g of purified water, heating to 80 ℃, preserving heat, stirring and dissolving uniformly to obtain a first mixed solution;
s108: taking 2.0g of glycerol, 3.5g of propylene glycol, 0.15g of methylparaben, 0.1g of phenoxyethanol, 0.2g of ethylhexyl glycerol and 0.5g of PEG-40 hydrogenated castor oil according to the mass ratio of the raw materials and the auxiliary materials, heating the materials in a second steam heating interlayer tank to 80 ℃, and completely dissolving and uniformly mixing the materials to obtain a second mixed solution;
s109: and (3) slowly adding the mixed solution prepared in the step (S108) into the first mixed solution in the interlayer tank of the step (S107) through a sterile pipeline, uniformly stirring, carrying out heat preservation treatment for 10min, cooling to 35 ℃, sequentially adding the macadimia nut protein peptide solution prepared in the step (4.0 g S101) and the macadimia nut green husk extracting solution prepared in the step (2.0 g S102) and 0.015g of sage essential oil, uniformly stirring, and then clarifying through a disk centrifuge to finally obtain the macadimia nut smoothing toner.
Example four: a preparation method of smoothing toner with macadamia nut protein peptide and green tangerine peel extract as active ingredients comprises the following steps:
s107: taking 2.0g of trehalose, 0.5g of allantoin, 2.0g of hyaluronic acid and 0.2g of xanthan gum according to the mass ratio of raw materials and auxiliary materials in a first steam heating interlayer tank, adding 83.59g of purified water, heating to 80 ℃, preserving heat, stirring and dissolving uniformly to obtain a first mixed solution;
s108: taking 2.0g of glycerol, 3.0g of propylene glycol, 0.1g of methyl hydroxybenzoate, 0.1g of phenoxyethanol, 0.1g of ethylhexyl glycerol and 0.4g of PEG-40 hydrogenated castor oil according to the mass ratio of the raw materials and the auxiliary materials, heating the mixture in a second steam heating interlayer tank to 80 ℃, and completely dissolving and uniformly mixing the mixture to obtain a second mixed solution;
s109: and (3) slowly adding the mixed solution prepared in the step (S108) into the first mixed solution in the interlayer tank of the step (S107) through a sterile pipeline, uniformly stirring, carrying out heat preservation treatment for 10min, cooling to 35 ℃, sequentially adding the macadimia nut protein peptide solution prepared in the step (2.0 g S101) and the macadimia nut green husk extracting solution prepared in the step (4.0 g S102) and 0.01g of sage essential oil, uniformly stirring, and then clarifying through a disk centrifuge to finally obtain the macadimia nut smoothing toner.
Example five: a preparation method of smoothing toner with macadamia nut protein peptide and green tangerine peel extract as active ingredients comprises the following steps:
s107: taking 3.5g of trehalose, 1.5g of allantoin, 4.0g of hyaluronic acid and 0.5g of xanthan gum according to the mass ratio of raw materials and auxiliary materials, adding 72.19g of purified water into a first steam heating interlayer tank, heating to 80 ℃, preserving heat, stirring and dissolving uniformly to obtain a first mixed solution;
s108: taking 5.0g of glycerol, 6.0g of propylene glycol, 0.2g of methylparaben, 0.2g of phenoxyethanol, 0.3g of ethylhexyl glycerol and 0.6g of PEG-40 hydrogenated castor oil according to the mass ratio of the raw materials and the auxiliary materials, heating the mixture to 80 ℃, and completely dissolving and uniformly mixing the mixture to obtain a second mixed solution;
s109: and (3) slowly adding the mixed solution prepared in the step (S108) into the first mixed solution in the interlayer tank of the step (S107) through a sterile pipeline, uniformly stirring, carrying out heat preservation treatment for 10min, cooling to 35 ℃, sequentially adding the macadimia nut protein peptide solution prepared in the step (3.0 g S101) and the macadimia nut green husk extract solution prepared in the step (3.0 g S102) and 0.01g of sage essential oil, uniformly stirring, and then clarifying through a disk centrifuge to finally obtain the macadimia nut smoothing toner.
Example six: the difference between the sixth example and the third example is that the macadimia nut green husk extract prepared in the step 2.0g S102 and the macadimia nut protein peptide liquid prepared in the step 6.0g S101 are added in the step S109.
Example seven: the difference between the seventh example and the third example is that deionized water of equivalent weight is added in place of the macadamia nut protein peptide solution in step S109.
Example eight: the difference between the eighth embodiment and the third embodiment is that deionized water of the same weight is added in the step S109 instead of the extract of green husk of macadimia nut.
Example nine: the difference between the ninth embodiment and the third embodiment is that deionized water with the same weight is added in the step S109 instead of the macadimia nut protein peptide liquid and the macadimia nut green husk extract liquid.
Testing and evaluating the skin softening aquatic product obtained by the experiment:
1. stability test
The skin lotions of the third to ninth examples were put into a transparent tube and observed under the same natural light, and after 0 day, 1 day and 3 days, the pH thereof was measured and the changes of the clarity, color and delamination were observed, respectively, with the results shown in tables 2 and 3.
TABLE 2 stability under illumination at different times
As shown in the results in table 2, the skin softening water system prepared in this embodiment, which uses macadamia nut protein peptide and green tangerine orange peel extract as active ingredients and uses moisturizing factors, preservatives, bacteriostats and essences as auxiliary materials, is stable under light conditions and can be used for exposed skin. However, the single macadimia nut protein peptide component or the single macadimia nut green husk extract goes bad after 3 days of illumination, and the reason may be that amino groups exposed by amino acids of a protein peptide part or hydroxyl groups exposed by polyphenol are easy to generate oxidation reaction, so that the system balance is damaged.
TABLE 3 stability test and evaluation of the skin softening water of example III under different temperature conditions
The stability of the smoothing toner prepared in the third to sixth examples was evaluated by testing using the conventional test standard for the smoothing toner, as shown in table 3, the smoothing toner of the third example has stable performance at a temperature of-15 ℃ to 40 ℃, has low environmental requirements, and can be popularized and used in various regions around the world.
2. Moisturizing and skin elasticity test
Selecting 24 volunteers for participation, wherein the volunteers are 20-30 years old and randomly distributed, grouping three volunteers, and all the volunteers have no abnormal parts and are not smeared with any external preparation, firstly cleaning the hands with detergent and wiping the hands dry, sitting still for 30min to keep the temperature and humidity of the skin balanced with the environment, selecting 4 experimental areas of 3 x 3 on the inner side of each arm, wherein the dosage of each area is 3g, respectively smearing 1mL on the experimental areas in a room with constant humidity (50%) and temperature (25 ℃), and clockwise rotating for 30s at the speed of 1 r/s. Testing the moisture content by using a moisture tester produced by American CK company, taking the average value of a test area, measuring the average value of the moisture content once every 0h (before applying smoothing toner) and 0.5h, 1h, 2h, 3h and 4h after applying smoothing toner, and comparing the change of the average value of the moisture content so as to evaluate the moisture content, wherein the moisture content test result is shown in a table 4; applying the coating to the same experiment area of the same experiment volunteer for three times in the morning, at noon and evening, wherein each area is 3g in dosage, continuously applying for 0 day, 3 days, 7 days, 14 days, 30 days and 60 days, and performing elasticity value test by using an elasticity tester, wherein the elasticity test result is shown in table 5.
Table 4 moisturizing performance test results of the lotions of examples three to nine
TABLE 5 skin elasticity test results after smoothing toner using examples three to nine
As shown in the results of table 4 and table 5, in the third example, the effect of mixing the macadimia nut protein peptide solution and the macadimia nut green husk extract solution with the skin softening water consisting of the moisturizing factor, the preservative and the like is most obvious on the skin moisturizing effect and the improvement of skin elasticity, especially when the ratio of the macadimia nut protein peptide solution to the macadimia nut green husk extract solution is 2:1, the absorption is fast and the moisturizing time is long, and considering the cost problem of the protein peptide, the content is increased but the effect is rather reduced, and the protein peptide is not adopted in the actual production.
3. Antioxidant effect
The experiment for measuring the elimination of the free radicals by the smoothing toner in the experiment has two types:
DPPH free radical scavenging ability test: the concentration of the mixture is 2.0 multiplied by 10 by absolute ethyl alcohol-4Storing the solution of DPPH in mol/L in dark for later use. Mixing 2mL of DPPH solution and 2.0mL of extraction solvent in a stoppered test tube, and measuring absorbance A at 517nm with the extraction solvent as reference solution for 30min0(ii) a The smoothing toner, the commercial smoothing toner and the Trol of the third to ninth examples are respectively taken2.0mL of ox solution and 2mL of DPPH solution in a stoppered tube, absorbance A was measured under the above conditions1The assay was performed 3 times in parallel. The DPPH free radical clearance rate calculation formula is as follows:
ABTS free radical elimination capability test: preparing 7mmol/L ABTS and 2.45m mol/L K with distilled water respectively2S2O8The solution is mixed with the two solutions in a ratio of 1:1 and is reacted for 14 hours in a dark place to obtain ABTS+Solution, dilution of ABTS with extraction solvent+The light absorption value of the solution at 732nm is 0.70 +/-0.02 for later use; take 50 μ L and 4mL ABTS of the smoothing toner prepared in examples three to nine, the commercial smoothing toner, Trolox solution+Placing the solution in a test tube with a plug, performing water bath at 37 deg.C for 10min, and measuring absorbance A under the above conditions1(ii) a Measuring absorbance A at 732nm with extraction solvent as reference solution0The ABTS free radical clearance rate is calculated by the formula of 3 times of parallel measurement:
the elimination results are shown in Table 6.
TABLE 6 free radical scavenging ability test
As shown in Table 6, the skin softening water of example III has strong capability of eliminating DPPH free radicals and ABTS free radicals, which is not the sum of the capabilities of example seven and example eight, while the skin softening water of example six does not improve the free radical elimination capability of the skin softening water obviously by simply increasing the content of nut protein peptide. The protein peptide and the green tangerine orange peel extract in proper proportion in the smoothing toner have certain synergistic effect.
4. Allergy-relieving therapeutic effect
8 allergic patients were selected and divided into two groups, and the face had desquamation, erythema and itching symptoms, and the smoothing toner prepared in the third example was applied to the allergic patients for 1 day, 7 days and 30 days in succession, and the product was sprayed on the face twice a day after cleansing the face, and the effect was observed, and the test results are shown in table 7.
TABLE 7 allergy relief Effect test
As shown in Table 7, the smoothing toner produced by the third embodiment of the invention has the effects of relieving and even preventing skin allergy, especially desquamation, erythema and pruritus.
In conclusion, the macadimia nut protein peptide smoothing toner disclosed by the invention takes the macadimia nut protein peptide and the macadimia nut green husk extracting solution as active ingredients, can play a good role in moisturizing, resisting wrinkles and inhibiting and preventing skin aging on facial skin, does not irritate the facial skin, and is simple in preparation method process, low in investment cost and suitable for mass production.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (3)
1. A smoothing toner with macadamia nut protein peptide and green tangerine peel extract as active ingredients is characterized in that: the material is prepared from the following raw materials in percentage by weight:
wherein the macadamia nut protein peptide is prepared by the following steps:
step S101: weighing 5.000g of macadimia nut protein powder, dissolving in 100mL of ultrapure water, adjusting the pH of the solution to 10.0 by using 1.0mol/L sodium hydroxide, adding protease accounting for 5.0% of the weight of the macadimia nut protein powder, and uniformly mixing; the protease comprises alkaline protease and compound protease with the mass ratio of 3: 1;
step S102: adopting dynamic high-pressure microjet technology to assist enzymolysis of the solution in the step S101, wherein the treatment pressure is 50MPa, the treatment time is 60min, after the enzymolysis is finished, carrying out enzyme deactivation treatment for 15min in water bath at 80 ℃, cooling, and then adjusting the pH of the enzymolysis solution to 7.0 by using 1.0mol/L hydrochloric acid;
step S103: centrifuging the step S102 by using a disk centrifuge, performing ultrafiltration treatment on the obtained clear solution by using a 3kDa ultrafiltration membrane, controlling the ultrafiltration flow rate to be 2.0ml/min, pressurizing by using nitrogen, and concentrating the ultrafiltration solution with the molecular weight of less than 3kDa to be 0.2 times of the original solution by using reduced pressure distillation to obtain concentrated solution, namely the macadamia nut protein peptide;
the macadimia nut green husk extracting solution is prepared by the following steps:
step S104: vacuum freeze drying fresh macadimia nut green peel for 16h until the moisture content is less than 3.0%, and then pulverizing the dried macadimia nut green peel into material particles smaller than 25 microns by adopting an ultramicro pulverizing technology for later use;
step S105: taking 100g of the macadimia nut green husk powder, adding a 60% ethanol solution according to the weight ratio of 1:20, treating for 5min at 40MPa by using a dynamic high-pressure micro-jet homogenizer, then leaching for 80min in a water bath at 55 ℃, and then clarifying by using a disk centrifuge, wherein the clarified liquid is the initial liquid of the macadimia nut green husk extraction;
step S106: and concentrating the initial liquid of the macadimia nut green husk extractive solution obtained in the step S105 to 0.1 time of the volume of the initial liquid through reduced pressure distillation to obtain the macadimia nut green husk extractive solution.
2. The smoothing toner as claimed in claim 1, wherein the smoothing toner comprises macadamia nut protein peptide and green tangerine orange peel extract as active ingredients, and is characterized in that: the material is prepared from the following raw materials in percentage by weight:
3. the method of claim 1 or 2, wherein the method comprises the steps of: the method comprises the following steps:
step S107: taking trehalose, allantoin, hyaluronic acid and xanthan gum according to the mass ratio of the raw materials and the auxiliary materials, adding a certain amount of purified water into a steam heating interlayer tank, heating to 80 ℃, preserving heat, stirring and dissolving uniformly to obtain a first mixed solution;
step S108: according to the mass ratio of the raw materials to the auxiliary materials, putting glycerol, propylene glycol, methyl hydroxybenzoate, phenoxyethanol, ethylhexyl glycerol and PEG-40 hydrogenated castor oil into another steam heating interlayer tank, heating to 80 ℃, and completely dissolving and uniformly mixing to obtain a second mixed solution;
step S109: and (3) slowly adding the second mixed solution obtained in the step (S108) into the first mixed solution obtained in the step (S107) through a sterile pipeline, carrying out heat preservation treatment for 10min, cooling to 35 ℃, sequentially adding macadamia nut protein peptide, macadamia nut green husk extracting solution and sage essential oil according to the mass ratio of the raw materials and the auxiliary materials, uniformly stirring, and carrying out clarification treatment through a disc centrifuge to obtain the smoothing toner taking the macadamia nut protein peptide and the green husk extracting solution as active ingredients.
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| CN110693030A (en) * | 2019-10-31 | 2020-01-17 | 江城中澳农业科技发展有限公司 | Preparation method and application of macadimia nut green husk extract |
| CN112080543B (en) * | 2020-09-26 | 2024-06-28 | 协和生物技术(广东)有限公司 | Plant-derived polypeptide with anti-aging effect, face cream containing plant-derived polypeptide and preparation method of face cream |
| CN112674199B (en) * | 2020-12-09 | 2021-07-06 | 广州市金龟寿药品有限公司 | Polypeptide food preservative and preparation method thereof |
| CN113230174B (en) * | 2021-05-28 | 2022-07-08 | 西南林业大学 | Macadamia nut whitening extract and preparation method thereof |
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