CN107853709A - A kind of Queensland nut polypeptide production methods - Google Patents

A kind of Queensland nut polypeptide production methods Download PDF

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Publication number
CN107853709A
CN107853709A CN201711011417.8A CN201711011417A CN107853709A CN 107853709 A CN107853709 A CN 107853709A CN 201711011417 A CN201711011417 A CN 201711011417A CN 107853709 A CN107853709 A CN 107853709A
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CN
China
Prior art keywords
queensland nut
polypeptide
preparation
freeze
queensland
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CN201711011417.8A
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Chinese (zh)
Inventor
陈进基
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Maoming Dianbai Food Co Ltd
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Maoming Dianbai Food Co Ltd
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Publication date
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Priority to CN201711011417.8A priority Critical patent/CN107853709A/en
Publication of CN107853709A publication Critical patent/CN107853709A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Cereal-Derived Products (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention discloses a kind of Queensland nut polypeptide production methods.The Queensland nut polypeptide production methods of the present invention are the enzymolysis extracting polypeptides from Queensland nut, are passed through:A, crush;B, the homogeneous of suspension;C, constant temperature digests;D, boiling water sterilizes;E, centrifuge;F, it is freeze-dried, obtains Queensland nut polypeptide powder.The present invention prepares polypeptide by enzyme process can improve Queensland nut cake protein utilization rate.The polypeptide nature that the inventive method obtains is more stable, and yield is high relative to other drying modes.By the Queensland nut polypeptide molecular weight for preparing of the present invention is low, product stability is good, there is good antifatigue, hypotensive, anti-oxidant and other effects.

Description

A kind of Queensland nut polypeptide production methods
Technical field
The present invention relates to food processing field, and in particular to a kind of Queensland nut polypeptide production methods.
Background technology
Queensland nut rich in nutrition content, kernel is in addition to containing higher fat, also containing at a relatively high protein and carbon Hydrate, also rich in calcium, phosphorus, iron and B family vitamin thiamine, riboflavin and nicotinic acid.Macadimia nut oil has plurality kinds of health care Function, the grouts after oil expression remain the full nutriment of Queensland nut, have higher nutritive value, but be primarily now used as Feed, cause the significant wastage of resource, the accessory substance Queensland nut dregs of rice after oil expression contain the protein of high level, glutamic acid, Arginine, aspartic acid, leucine and lysine, it is to improve having for Queensland nut cake protein utilization rate to prepare polypeptide by enzyme process One of effect approach.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preparation method of Queensland nut polypeptide, after solving oil expression Queensland nut dregs of rice utilization rate is low, and protein resource wastes problem.For above-mentioned technical problem of determining, the technical solution adopted in the present invention It is:A kind of preparation method of Queensland nut polypeptide, comprises the following steps:
A, crush:Hydraulic pressure squeezes the Queensland nut dregs of rice and crosses 60 mesh sieves after the crushing of plant pulverizer, obtains Queensland nut dregs of rice powder;
B, the homogeneous of suspension:Queensland nut dregs of rice powder after crushing adds water to mix well, and is uniformly mixed liquid through homogenizer homogeneous;
C, constant temperature digests:Mixed liquor is preheated into 10min in boiling water, 50~60 DEG C is cooled to and adds protease, adjusts pH value extremely 7.0~8.5, constant temperature digests 6~8 hours, and enzymolysis liquid is made;
D, boiling water sterilizes:Enzymolysis liquid is incubated 5~8 minutes in boiling water bath;
E, centrifuge:Enzymolysis liquid Jing Guo enzyme deactivation is centrifuged into 10 min in centrifuge, takes supernatant;
F, it is freeze-dried:By supernatant cryogenic freezing 24 hours, it is freeze-dried to obtain Queensland nut in freeze drier Polypeptide powder.
In the step b, the mixing quality ratio of Queensland nut dregs of rice powder and water is 1:4~6.
In the step b, homogenization pressure is 25~30MPa.
Protease used is neutral proteinase in the step c, enzyme concentration 3200U/g.
It is -50 DEG C that condition is freeze-dried in the step f, 25Pa.
Advantage of the invention is that:Queensland nut rich in nutrition content, the nut dregs of rice after extracting oil equally remain wherein Most nutritional ingredient, it is to improve Queensland nut cake protein utilization rate to prepare polypeptide by enzyme process.It is hard that the present invention prepares Australia Fruit polypeptide is carried out by the way of freeze-drying, and obtained polypeptide nature is more stable, and yield is high relative to other drying modes. By the Queensland nut polypeptide molecular weight for preparing of the present invention is low, product stability is good, there is good antifatigue, hypotensive, antioxygen Change and other effects.
Embodiment
With reference to embodiment, the present invention will be further described:
A kind of preparation of Queensland nut polypeptide of embodiment 1, comprises the following steps:
A, crush:Hydraulic pressure squeezes the Queensland nut dregs of rice and crosses 60 mesh sieves after the crushing of plant pulverizer, obtains Queensland nut dregs of rice powder;
B, the homogeneous of suspension:Queensland nut dregs of rice powder after crushing adds water to mix well, and is uniformly mixed liquid through homogenizer homogeneous, Homogenization pressure is 25MPa;The mixing quality ratio of Queensland nut dregs of rice powder and water is 1:5;
C, constant temperature digests:Mixed liquor is preheated into 10min in boiling water, 50 DEG C is cooled to and adds neutral proteinase, enzyme concentration is 3200U/g, adjusting pH value, constant temperature digests 8 hours, and enzymolysis liquid is made to 7.0;
D, boiling water sterilizes:Enzymolysis liquid is incubated 5 minutes in boiling water bath;
E, centrifuge:By the enzymolysis liquid Jing Guo enzyme deactivation in centrifuge(4000 r/min)10 min are centrifuged, take supernatant;
F, it is freeze-dried:By supernatant cryogenic freezing 24 hours, it is freeze-dried to obtain Queensland nut in freeze drier Polypeptide powder, freeze-drying condition are -50 DEG C, 25Pa.
A kind of preparation of Queensland nut polypeptide of embodiment 2, comprises the following steps:
A, crush:Hydraulic pressure squeezes the Queensland nut dregs of rice and crosses 60 mesh sieves after the crushing of plant pulverizer, obtains Queensland nut dregs of rice powder;
B, the homogeneous of suspension:Queensland nut dregs of rice powder after crushing adds water to mix well, and is uniformly mixed liquid through homogenizer homogeneous, Homogenization pressure is 30MPa;The mixing quality ratio of Queensland nut dregs of rice powder and water is 1: 6;
C, constant temperature digests:Mixed liquor is preheated into 10min in boiling water, 50 DEG C is cooled to and adds neutral proteinase, enzyme concentration is 3200U/g, adjusting pH value, constant temperature digests 6 hours, and enzymolysis liquid is made to 8.5;
D, boiling water sterilizes:Enzymolysis liquid is incubated 8 minutes in boiling water bath;
E, centrifuge:By the enzymolysis liquid Jing Guo enzyme deactivation in centrifuge(5000 r/min)10 min are centrifuged, take supernatant;
F, it is freeze-dried:By supernatant cryogenic freezing 24 hours, it is freeze-dried to obtain Queensland nut in freeze drier Polypeptide powder, freeze-drying condition are -50 DEG C, 25Pa.

Claims (5)

  1. A kind of 1. preparation method of Queensland nut polypeptide, it is characterised in that:Comprise the following steps:
    A, crush:Hydraulic pressure squeezes the Queensland nut dregs of rice and crosses 60 mesh sieves after the crushing of plant pulverizer, obtains Queensland nut dregs of rice powder;
    B, the homogeneous of suspension:Queensland nut dregs of rice powder after crushing adds water to mix well, and is uniformly mixed liquid through homogenizer homogeneous;
    C, constant temperature digests:Mixed liquor is preheated into 10min in boiling water, 50~60 DEG C is cooled to and adds protease, adjusts pH value extremely 7.0~8.5, constant temperature digests 6~8 hours, and enzymolysis liquid is made;
    D, boiling water sterilizes:Enzymolysis liquid is incubated 5~8 minutes in boiling water bath;
    E, centrifuge:Enzymolysis liquid Jing Guo enzyme deactivation is centrifuged into 10 min in centrifuge, takes supernatant;
    F, it is freeze-dried:By supernatant cryogenic freezing 24 hours, it is freeze-dried to obtain Queensland nut in freeze drier Polypeptide powder.
  2. A kind of 2. preparation method of Queensland nut polypeptide according to claim 1, it is characterised in that:In step b, Australia The mixing quality ratio of nut dregs of rice powder and water is 1:4~6.
  3. A kind of 3. preparation method of Queensland nut polypeptide according to claim 1, it is characterised in that:Homogeneous pressure in step b Power is 25~30MPa.
  4. A kind of 4. preparation method of Queensland nut polypeptide according to claim 1, it is characterised in that:Egg used in step c White enzyme is neutral proteinase, enzyme concentration 3200U/g.
  5. A kind of 5. preparation method of Queensland nut polypeptide according to claim 1, it is characterised in that:Freezed in step f dry Dry condition is -50 DEG C, 25Pa.
CN201711011417.8A 2017-10-26 2017-10-26 A kind of Queensland nut polypeptide production methods Pending CN107853709A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711011417.8A CN107853709A (en) 2017-10-26 2017-10-26 A kind of Queensland nut polypeptide production methods

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711011417.8A CN107853709A (en) 2017-10-26 2017-10-26 A kind of Queensland nut polypeptide production methods

Publications (1)

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CN107853709A true CN107853709A (en) 2018-03-30

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108670901A (en) * 2018-06-13 2018-10-19 中国热带农业科学院环境与植物保护研究所 It is a kind of using Queensland nut protein peptides and green peel extracting solution as smoothing toner of active constituent and preparation method thereof
CN108796015A (en) * 2018-06-13 2018-11-13 中国热带农业科学院南亚热带作物研究所 A method of synchronizing extraction protein peptides and vegetable fat from Queensland nut
CN111657468A (en) * 2020-05-27 2020-09-15 安徽乐方食品有限公司 Processing method for enriching antioxidant polypeptide from nut kernels by enzymatic hydrolysis of enzyme preparation
CN111662798A (en) * 2020-06-24 2020-09-15 云南省热带作物科学研究所 Macadamia nut wine with high antioxidant function and preparation method thereof
CN112674199A (en) * 2020-12-09 2021-04-20 广州市金龟寿药品有限公司 Polypeptide food preservative and preparation method thereof
CN113575904A (en) * 2021-07-21 2021-11-02 广西南亚热带农业科学研究所 Mustard-flavored Australia nut paste and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779536A (en) * 2016-01-13 2016-07-20 张新宇 Preparation process of almond blood pressure reducing functional oligopeptides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779536A (en) * 2016-01-13 2016-07-20 张新宇 Preparation process of almond blood pressure reducing functional oligopeptides

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108670901A (en) * 2018-06-13 2018-10-19 中国热带农业科学院环境与植物保护研究所 It is a kind of using Queensland nut protein peptides and green peel extracting solution as smoothing toner of active constituent and preparation method thereof
CN108796015A (en) * 2018-06-13 2018-11-13 中国热带农业科学院南亚热带作物研究所 A method of synchronizing extraction protein peptides and vegetable fat from Queensland nut
CN108670901B (en) * 2018-06-13 2021-03-23 中国热带农业科学院环境与植物保护研究所 Skin softening lotion with macadamia nut protein peptide and green tangerine peel extract as active ingredients and preparation method thereof
CN111657468A (en) * 2020-05-27 2020-09-15 安徽乐方食品有限公司 Processing method for enriching antioxidant polypeptide from nut kernels by enzymatic hydrolysis of enzyme preparation
CN111662798A (en) * 2020-06-24 2020-09-15 云南省热带作物科学研究所 Macadamia nut wine with high antioxidant function and preparation method thereof
CN112674199A (en) * 2020-12-09 2021-04-20 广州市金龟寿药品有限公司 Polypeptide food preservative and preparation method thereof
CN113575904A (en) * 2021-07-21 2021-11-02 广西南亚热带农业科学研究所 Mustard-flavored Australia nut paste and preparation method thereof

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Application publication date: 20180330