CN104115989A - Marine fish peptide powder, preparation method and applications thereof - Google Patents
Marine fish peptide powder, preparation method and applications thereof Download PDFInfo
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- CN104115989A CN104115989A CN201410338332.0A CN201410338332A CN104115989A CN 104115989 A CN104115989 A CN 104115989A CN 201410338332 A CN201410338332 A CN 201410338332A CN 104115989 A CN104115989 A CN 104115989A
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Abstract
The invention provides a preparation method of fish meal protein peptide. The preparation method comprises the following steps: (1) taking degreased marine fish meal as the raw material, dissolving the fish meal in water according to a weight/volume ratio of 1:5-12.5; (2) boiling for 20 to 30 minutes at a temperature of 115 to 130 DEG C under a pressure of 0.75 to 1.75 kg/cm2, cooling so as to obtain a boiled liquid; (3) adjusting the pH value of the boiled liquid to 7.0-9.5, adding protease, controlling the mass concentration ratio of the protease to the substrate to be 0.5 to 2.5%, carrying out enzymolysis at a temperature of 45 to 70 DEG C so as to obtain enzymatic hydrolysate; (4) heating the enzymatic hydrolysate to 90 to 95 DEG C to inactivate the enzymes, adding active carbon, maintaining the temperature, continuously stirring at the same time so as to carry out decoloring and deodorization, then transporting the processed enzymatic hydrolysate into a plate-and-frame filter press to carry out filter pressing, condensing the clarified filtrate, and finally spray-drying so as to obtain the fish meal protein peptide. The prepared fish meal protein peptide is rich in nutrients, is easy to digest and absorb, and can strengthen the immunity. The nutrition value and biological utilization rate of low-market-value marine fishes are prominently improved. The protein peptide is suitable for preparation of various kinds of functional healthcare foods or nutrition foods.
Description
Technical field
The invention belongs to marine fish deep process technology field, be specifically related to a kind of marine fish Gly-His-Lys and preparation method thereof and application.
Background technology
Fish meal is by one or more marine fishes, particularly using the small fish of non-edible part as raw materials for production, and the high protein powder obtaining after a series of processing such as boiling, squeezing, dehydration and pulverizing.Fish meal becomes protein sources the most frequently used in aquatic feeds because it is best in quality.Fish meal is abundant with its essential amino acids content, amino acid Compositional balance, protein content are high, good palatability, digestibility are high and it contains compared with high unrighted acid and the feature such as ANFs is few and becomes the most frequently used most important animal protein source in aquatic feeds.Protein is one of nutriment the most key in growth of animal process.The protein that animal obtains from food is decomposed into amino acid under the effect of the white enzyme of plurality of enzymes, is absorbed in vivo, then compound body protein or decomposition produce power in alimentary canal.But, modern study is found: the protein of animal picked-up is after the enzymolysis of alimentary canal protease, before not resembling, think and only absorb with amino acid whose form, also directly absorb with the form of low peptide, and the infiltration rate of dipeptides and tripeptides is faster than the amino acid of same composition.
China's fish meal annual production has reached 1,000,000 tons of left and right, becomes maximum in the world fish meal producing country and country of consumption.But, though be fish meal big producing country, the difficult production power that claims, regional small enterprise of China is more, production scale is generally less, industrial concentration is low, causes production technology and product quality level poor.How to be upgraded and to be realized specialization, scale, intensive production by integration, improve production technology level, by utilizing expeditiously limited resource, creating larger value is China's fish meal processing industry problem demanding prompt solution.
Summary of the invention
The object of the present invention is to provide one to possess nutritional labeling abundant, the preparation method of the marine fish Gly-His-Lys of the physiological functions such as easy to digest, easy absorption, enhancing immunity.
For achieving the above object, the present invention adopts following technological means:
A preparation method for marine fish Gly-His-Lys, comprises the steps:
(1) taking degreasing ocean fish meal as raw material, the amount that is 1:5～12.5 by feed liquid w/v adds water-soluble solution;
(2) at 115～130 DEG C of temperature, pressure 0.75～1.75kg/cm
2lower boiling 20～30 minutes; Cooling, obtain cooking liquor;
(3) adjust cooking liquor pH value to pH7.0～9.5; Add protease, the mass concentration ratio of controlling albumen enzyme-to-substrate is 0.5～2.5%, and enzymolysis at 45～70 DEG C, obtains enzymolysis liquid;
(4) enzymolysis liquid is warming up to 90～95 DEG C, deactivation, adds 1～4g/L active carbon, and insulation is also constantly stirred, and decoloration deodorization, then enters plate and frame filter press, and pressing filtering liquid is concentrated, and spraying is dry, to obtain final product.The marine fish Gly-His-Lys that above-mentioned preparation method makes is 65.50～82.69% containing crude protein content, and oligopeptide, containing 55～75.52%, can be used as food additives, is applied to each nutritious food, beverage and flavoring.
Further, the present invention also provides above-mentioned preparation method's optimum condition, as:
In preferred steps (1), feed liquid w/v is 1:10, fully contacts with fish meal protein matter to realize protease.
In preferred steps (2), pressure is 1～1.5kg/cm
2, 121 DEG C of temperature, the time is 24～28 minutes, is fully degraded into peptide class to obtain fish meal protein.
Wherein, described protease is the mixture of alkali protease or alkali protease and flavor protease, and wherein, described alkali protease is 0.5～2.5% with concentration of substrate ratio, and flavor protease and concentration of substrate are 1～2.5%.Alkali protease and flavor protease are known protease, are commercially available prod, and wherein alkali protease is preferably purchased from Wuxi Jie Nengke bio-engineering corporation, and flavor protease is preferably purchased from Novozymes Company of Denmark.Described alkali protease more preferably adopts Novozymes Company of alkali protease Alcalase 2.4L(Denmark).In the time adopting the two mixture, the amount ratio of alkali protease and flavor protease is preferably but not limited to 1:1.
In preferred steps (3), adjust cooking liquor pH value to 9.0.Under this pH value, can be conducive to the hydrolysis of alkali protease to fish meal protein.
The temperature of preferred described enzymolysis is 60 DEG C, to facilitate enzymolysis process better.
Preferred described enzymolysis time is 2-4 hour, to reach higher degree of hydrolysis.
Wherein, in described step (4), enzymolysis liquid is warming up to 90～95 DEG C, deactivation 10min, add 2g/L active carbon, at 50 DEG C, insulation decolouring 40～60min constantly stirring, then utilize plate and frame filter press press filtration, and pressure filtrate is concentrated into solid content 10～15%, spraying is dry, drying condition is inlet temperature 110-230 DEG C, and outlet temperature 70-90 DEG C, to obtain final product.This step can further be removed fishy smell, bitter taste and the pigment in fish meal hydrolyzate, the fish Gly-His-Lys that makes to obtain possess without fishy smell bitter taste, color and luster be creamy white, the characteristic such as soluble in water, delicate flavour is strong.
Degreasing of the present invention ocean fish meal is that deep-sea Optimization of Low Value Fish is through the low-temperature defatted fish protein powder of making, by those skilled in the art are grasped.Preferably control protein content 70-77% in the fish meal of degreasing ocean, moisture≤7.0%, Residual oil≤1.0%.Wherein, concrete preparation method can adopt prior art disclosed multiple, adds vapour modifier treatment → make fish particulate material → dipping extraction → fat protein separation → finished product fish meal as Optimization of Low Value Fish → roughing becomes fish meal → heating.Described low value fish are as Anchovies fish etc.
The second object of the present invention is the marine fish Gly-His-Lys of protecting above-mentioned preparation method to obtain, and it is abundant that this marine fish Gly-His-Lys possesses nutritional labeling, and the physiological functions such as easy to digest, easy absorption, enhancing immunity, can be widely used in food additives.
The 3rd object of the present invention is to protect above-mentioned marine fish Gly-His-Lys as the application in food additives, because this product Small Peptides, amino acid content are higher, therefore, is prepared into after food additives, can be conducive to human body and digest and assimilate.Concrete addition is grasped by those skilled in the art, and the present invention is not particularly limited this.
In addition, the present invention also provides the application of above-mentioned marine fish Gly-His-Lys as food additives.Wherein marine fish Gly-His-Lys can be used as food additives and joins in food, to improve nutritive value and the bioavilability of marine low-value fish.Wherein, described food can be person in middle and old age's nourishing reinforced food, comprises bread, cake etc., and the addition of marine fish Gly-His-Lys is grasped by those skilled in the art, and the present invention is not particularly limited this.
The invention has the advantages that: 1) adopt HIGH PRESSURE TREATMENT fish meal raw material, be conducive to improve the solubility of the protein of the leftover bits and pieces such as fish-bone, fish scale in fish meal; 2) adopt proteasome degradation method to produce active peptide, reaction condition gentleness, enzymolysis process is easily controlled, environmentally friendly; 3) improve the trophic component of fish meal, in product to be easy to oligopeptide that body absorbs as main.
The present invention makes full use of the existing abundant seawater low value stock of fish of China, taking the low value fish meal of degreasing as raw material, utilize protease hydrolyzed to prepare Gly-His-Lys, can improve the functional characteristic of fish meal protein, improve the nutritive value of fish meal, for new approach is opened up in the exploitation of China's marine low-value fish protein.
Detailed description of the invention
Embodiment 1
Taking degreasing ocean fish meal as raw material, add water and mix according to 1:10 material-water ratio, at 121 DEG C, 1.05kg/cm
2boiling 25 minutes under pressure, takes out and is cooled to 60 DEG C.Adjust cooking liquor pH value to 9, taking Novozymes Company of alkali protease Alcalase 2.4L(Denmark) as enzymolysis enzyme, controlling enzyme-to-substrate concentration ratio is 1%, at 60 DEG C, carry out enzymolysis, and it is stable to remain in enzymolysis process pH, after enzymolysis finishes, is warming up to 92 DEG C, deactivation 10 minutes, add 2g/L active carbon, insulation is also constantly stirred decoloration deodorization, then enter plate and frame filter press, pressure filtrate is concentrated into solid content 12%, and spraying is dry, and drying condition is 150 DEG C of inlet temperatures, 80 DEG C of outlet temperatures, to obtain final product.
After testing, in the prepared marine fish Gly-His-Lys of the present embodiment, oligopeptide content is 75.50%, and oligomeric Gly-His-Lys relative molecular mass, all lower than 1370Da, is a kind of high-quality marine fish Gly-His-Lys.
Embodiment 2
Taking degreasing ocean fish meal as raw material, add water and mix according to 1:5 material-water ratio, at 121 DEG C, 1.05kg/cm
2boiling 20 minutes under pressure, takes out and is cooled to 60 DEG C.Adjust cooking liquor pH value to 7.0, taking flavor protease (Novozymes Company of Denmark) as enzymolysis enzyme, controlling enzyme-to-substrate concentration ratio is 2.0%, at 55 DEG C, carry out enzymolysis, and it is stable to remain in enzymolysis process pH, after enzymolysis finishes, be warming up to 90 DEG C, deactivation 10 minutes, adds 4g/L active carbon, insulation is also constantly stirred, and decoloration deodorization, then enters plate and frame filter press, pressure filtrate is concentrated into solid content 10%, and spraying is dry, and drying condition is 180 DEG C of inlet temperatures, 90 DEG C of outlet temperatures, to obtain final product.
After testing, in the prepared marine fish Gly-His-Lys of the present embodiment, containing oligopeptide content is 62.00%, and oligomeric Gly-His-Lys relative molecular mass, lower than 1420Da, is a kind of high-quality marine fish Gly-His-Lys.
Embodiment 3
Taking degreasing ocean fish meal as raw material, add water and mix according to 1:12.5 material-water ratio, at 130 DEG C, 1.75kg/cm
2boiling 30 minutes under pressure, takes out and is cooled to 60 DEG C.Adjust cooking liquor pH value to 9, taking alkali protease (Wuxi Jie Nengke bio-engineering corporation) as enzymolysis enzyme, controlling enzyme-to-substrate concentration ratio is 2.5%, at 60 DEG C, carry out enzymolysis, and it is stable to remain in enzymolysis process pH, after enzymolysis finishes, be warming up to 95 DEG C, deactivation 10 minutes, adds 2g/L active carbon, insulation is also constantly stirred, and decoloration deodorization, then enters plate and frame filter press, pressure filtrate is concentrated into solid content 10%, and spraying is dry, and drying condition is 110 DEG C of inlet temperatures, 70 DEG C of outlet temperatures, to obtain final product.
After testing, in the prepared marine fish Gly-His-Lys of the present embodiment, oligopeptide content is 59.00%, and oligomeric Gly-His-Lys relative molecular mass, lower than 2080Da, is a kind of high-quality marine fish Gly-His-Lys.
Embodiment 4
Taking degreasing ocean fish meal as raw material, add water and mix according to 1:7.5 material-water ratio, at 125 DEG C, 1kg/cm
2boiling 24 minutes under pressure, takes out and is cooled to 60 DEG C.Adjust cooking liquor pH value to 8.5, first taking alkali protease Alcalase2.4L as enzymolysis enzyme, controlling enzyme-to-substrate concentration ratio is 0.5%, at 55 DEG C, carry out enzymolysis 2h, then regulate pH value to 7.0, add flavor protease, controlling enzyme-to-substrate concentration ratio is 0.5%, at 55 DEG C, carry out enzymolysis 2h, after enzymolysis finishes, be warming up to 95 DEG C, deactivation 10 minutes, add 3g/L active carbon, insulation is also constantly stirred, decoloration deodorization, then enter plate and frame filter press, pressure filtrate is concentrated into solid content 15%, spraying is dry, drying condition is 230 DEG C of inlet temperatures, 90 DEG C of outlet temperatures, obtain.
After testing, in the prepared marine fish Gly-His-Lys of the present embodiment, oligopeptide content is 69.93%, and oligomeric Gly-His-Lys relative molecular mass, lower than 1780Da, is a kind of high-quality marine fish Gly-His-Lys.
Embodiment 5
Using preparation-obtained embodiment 1 marine fish Gly-His-Lys as food additives, by 1% addition, fish Gly-His-Lys is joined in load weighted flour, salt, mix, abundant and face, static slaking, then through compressing tablet, slitting, the dry finished product vermicelli that contain fish Gly-His-Lys of obtaining.The nutritive value of gained vermicelli improves, and local flavor is more delicious.
Claims (9)
1. a preparation method for marine fish Gly-His-Lys, is characterized in that: comprise the steps:
(1) taking degreasing ocean fish meal as raw material, the amount that is 1:5～12.5 by feed liquid w/v adds water-soluble solution;
(2) at 115～130 DEG C of temperature, pressure 0.75～1.75kg/cm
2lower boiling 20～30 minutes; Cooling, obtain cooking liquor;
(3) adjust cooking liquor pH value to pH7.0～9.5; Add protease, the mass concentration ratio of controlling albumen enzyme-to-substrate is 0.5～2.5%, and enzymolysis at 45～70 DEG C, obtains enzymolysis liquid;
(4) enzymolysis liquid is warming up to 90～95 DEG C, deactivation, adds 1～4g/L active carbon, and insulation is also constantly stirred, and decoloration deodorization, then enters plate and frame filter press, and pressing filtering liquid is concentrated, and spraying is dry, to obtain final product.
2. preparation method according to claim 1, is characterized in that: in step (1), feed liquid w/v is 1:10.
3. preparation method according to claim 1, is characterized in that: in step (2), pressure is 1～1.5kg/cm
2, 121 DEG C of temperature, the time is 24～28 minutes.
4. preparation method according to claim 1, it is characterized in that: described protease is the mixture of alkali protease or alkali protease and flavor protease, wherein alkali protease is 0.5～2.5% with concentration of substrate ratio, and flavor protease and concentration of substrate are 1～2.5%.
5. preparation method according to claim 1, is characterized in that: in step (3), adjust cooking liquor pH value to 9.0.
6. preparation method according to claim 1, is characterized in that: in described step (4), enzymolysis liquid is warming up to 90～95 DEG C, deactivation 10min, add 2g/L active carbon, insulation decolouring 40～60min constantly stirring at 50 DEG C, decoloration deodorization, then enter plate and frame filter press, pressure filtrate is concentrated into solid content 10～15%, and spraying is dry, and drying condition is 110～230 DEG C of inlet temperatures, 70～90 DEG C of outlet temperatures, to obtain final product.
7. preparation method according to claim 1, is characterized in that: described degreasing ocean fish meal is that deep-sea Optimization of Low Value Fish is through the low-temperature defatted fish protein powder of making, protein content 55-75%, moisture≤10.0%, Residual oil≤1.0%.
8. the marine fish Gly-His-Lys that described in claim 1～7 any one, preparation method obtains.
Described in claim 8 marine fish Gly-His-Lys as the application of food additives.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063153A (en) * | 2015-09-21 | 2015-11-18 | 常熟理工学院 | Preparation method of food-grade low-salt-content sea fish oligopeptide powder |
| CN105154506A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Food-grade low-salinity ocean fish oligopeptide powder and application thereof |
| CN106234752A (en) * | 2016-07-25 | 2016-12-21 | 巢湖学院 | A kind of preparation method of instant Hemisalanx egg albumen powder |
| CN106260550A (en) * | 2016-08-28 | 2017-01-04 | 青岛海大生物集团有限公司 | A kind of fish peptide powder and preparation method thereof |
| CN106967770A (en) * | 2017-03-08 | 2017-07-21 | 浙江体育科学研究所 | A kind of method that polypeptide liquid is extracted from compound low value albacore |
| CN107279972A (en) * | 2017-06-23 | 2017-10-24 | 乐清益昌食品技术有限公司 | Compound based on Spanish mackerel active peptides |
| CN107893098A (en) * | 2018-01-12 | 2018-04-10 | 广州欧普康特医食品有限公司 | A kind of ocean fish oligopeptide and its preparation method and application |
| CN109529011A (en) * | 2019-01-08 | 2019-03-29 | 广东兴亿海洋生物工程股份有限公司 | Marine fishes oligopeptide has effects that the application in the drug, health care product or food that inhibit liver cancer cells in preparation |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN113174420A (en) * | 2021-04-23 | 2021-07-27 | 中海海洋科技股份有限公司 | Preparation method of high-yield marine fish oligopeptide product |
| CN113349356A (en) * | 2021-06-17 | 2021-09-07 | 烟台大学 | Iceland red-pole ginseng intestine egg nutritional jelly and preparation method thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154506B (en) * | 2015-09-21 | 2018-12-14 | 常熟理工学院 | A kind of food-grade low salt ocean fish oligopeptide powder and its application |
| CN105154506A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Food-grade low-salinity ocean fish oligopeptide powder and application thereof |
| CN105063153A (en) * | 2015-09-21 | 2015-11-18 | 常熟理工学院 | Preparation method of food-grade low-salt-content sea fish oligopeptide powder |
| CN105063153B (en) * | 2015-09-21 | 2018-09-11 | 常熟理工学院 | A kind of preparation method of food-grade low salt ocean fish oligopeptide powder |
| CN106234752A (en) * | 2016-07-25 | 2016-12-21 | 巢湖学院 | A kind of preparation method of instant Hemisalanx egg albumen powder |
| CN106260550A (en) * | 2016-08-28 | 2017-01-04 | 青岛海大生物集团有限公司 | A kind of fish peptide powder and preparation method thereof |
| CN106967770A (en) * | 2017-03-08 | 2017-07-21 | 浙江体育科学研究所 | A kind of method that polypeptide liquid is extracted from compound low value albacore |
| CN107279972A (en) * | 2017-06-23 | 2017-10-24 | 乐清益昌食品技术有限公司 | Compound based on Spanish mackerel active peptides |
| CN107893098A (en) * | 2018-01-12 | 2018-04-10 | 广州欧普康特医食品有限公司 | A kind of ocean fish oligopeptide and its preparation method and application |
| CN109529011A (en) * | 2019-01-08 | 2019-03-29 | 广东兴亿海洋生物工程股份有限公司 | Marine fishes oligopeptide has effects that the application in the drug, health care product or food that inhibit liver cancer cells in preparation |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN113174420A (en) * | 2021-04-23 | 2021-07-27 | 中海海洋科技股份有限公司 | Preparation method of high-yield marine fish oligopeptide product |
| CN113349356A (en) * | 2021-06-17 | 2021-09-07 | 烟台大学 | Iceland red-pole ginseng intestine egg nutritional jelly and preparation method thereof |
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Application publication date: 20141029 |