CN104082520B - A kind of low value marine fish Gly-His-Lys and its preparation method and application - Google Patents
A kind of low value marine fish Gly-His-Lys and its preparation method and application Download PDFInfo
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- CN104082520B CN104082520B CN201410338001.7A CN201410338001A CN104082520B CN 104082520 B CN104082520 B CN 104082520B CN 201410338001 A CN201410338001 A CN 201410338001A CN 104082520 B CN104082520 B CN 104082520B
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Abstract
The present invention provides the preparation method of a kind of fish meal protein peptide, specifically comprises the steps: that (1) is taking degreasing ocean fish meal as raw material, and the amount being 1:5��12.5 by feed liquid weightmeasurement ratio adds water dissolution; (2) boiling 20��30 minutes under temperature 115��130 DEG C, pressure 0.75��1.75kg/cm2; Cooling, obtains cooking liquor; (3) cooking liquor pH value is adjusted to pH7.0��9.5; Adding proteolytic enzyme, the mass concentration ratio of control albumen enzyme-to-substrate is 0.5��2.5%, and enzymolysis at 45��70 DEG C, obtains enzymolysis solution; (4) enzymolysis solution being warming up to 90��95 DEG C, deactivation, is drying to obtain. The fish-protein Gly-His-Lys prepared according to the present invention, small molecules oligopeptide content and digestibility height, as fodder additives and feed mixed feeding animal, it is possible to improve fish meal protein bioavailability, it is also possible to improve animal immunizing power, strengthens resistance against diseases.
Description
Technical field
The invention belongs to marine fish deep process technology field, it is specifically related to the preparation method of a kind of low value marine fish Gly-His-Lys.
Background technology
Fish meal is by one or more marine fishes, particularly using the raw fish of non-edible part as raw materials for production, and the high protein powder obtained after a series of processing such as boiling, squeezing, dehydration and pulverizing. Fish meal is best in quality and become protein source the most frequently used in aquatic feeds due to it. Fish meal is abundant with its essential amino acids content, amino acid Compositional balance, protein content height, good palatability, digestibility height and its contain higher unsaturated fatty acids and the feature such as antinutritional factor is few and become the most important the most frequently used animal protein source in aquatic feeds. Protein is one of nutritive substance the most key in growth of animal process. The protein that animal obtains from food is decomposed into amino acid under the effect of the white enzyme of multiple enzyme, is absorbed in the digested road of body, then synthesizes body protein or decomposes generate energy. But, modern study finds: the protein of animal picked-up is after the enzymolysis of digestive tube proteolytic enzyme, think before not resembling and only absorb with amino acid whose form, also directly absorb with the form of low peptide, and the absorption rate of dipeptides and tripeptides is faster than the amino acid of same composition.
China's fish meal annual production has reached about 1,000,000 tons, becomes fish meal producing country maximum in the world and consumption state. But, though fish meal big producing country, difficult title, produces power, regional small business of China is more, industrial scale generally less, industrial concentration is low, causes production technique and product quality level poor. How to realize specialization, mass-producing, intensive manufacture by integrating upgrading, it is to increase production technology level, utilizes limited resources by high-level efficiency, and creating bigger value is China's fish meal processing industry problem demanding prompt solution.
Summary of the invention
It is an object of the invention to provide one and possess rich in nutrition content, absorption easy to digest, easy, the preparation method strengthening the low value marine fish Gly-His-Lys of the physiological functions such as immunity.
For achieving the above object, the present invention adopts following technique means:
A preparation method for low value marine fish Gly-His-Lys, comprises the steps:
(1) taking degreasing ocean fish meal as raw material, the amount being 1:5��12.5 by feed liquid weightmeasurement ratio adds water dissolution;
(2) at temperature 115��130 DEG C, pressure 0.75��1.75kg/cm2Lower boiling 20��30 minutes; Cooling, obtains cooking liquor;
(3) cooking liquor pH value is adjusted to pH7.0��9.5; Add proteolytic enzyme, control albumen enzyme-to-substrate mass concentration ratio be 0.5��2.5%, enzymolysis at 45��70 DEG C, enzymolysis time be enzymolysis solution;
(4) enzymolysis solution being warming up to 90��95 DEG C, deactivation, further vacuum concentration, is drying to obtain.
The low value marine fish Gly-His-Lys crude protein content that above-mentioned preparation method obtains is 70.00-74.50%, and oligopeptide content 25%��30%, as fodder additives and feed mixed feeding animal, it is possible to effectively improve fish meal protein bioavailability, it is to increase animal disease resistant ability.
Further, present invention also offers the optimum condition of above-mentioned preparation method, as:
In preferred steps (1), feed liquid weightmeasurement ratio is 1:10, to realize fully contacting of protein and enzyme, it is to increase the degree of hydrolysis of protein.
In preferred steps (2), pressure is 1��1.5kg/cm2, temperature 121 DEG C, the time is 24��28 minutes, it is preferable that 25 minutes, the bone collagen sex change stripping being beneficial in fish meal.
Wherein, described proteolytic enzyme is Sumizyme MP, flavor protease or both mixtures, and proteolytic enzyme is 0.5��2.5% with concentration of substrate ratio. Described Sumizyme MP is such as Novozymes Company of Sumizyme MP Alcalase2.4L(Denmark), Sumizyme MP (Wuxi Jie Nengke bio-engineering corporation) and flavor protease (Novozymes Company of Denmark), when both employings mixture, the amount ratio 1:1 of Sumizyme MP and flavor protease. More preferably described proteolytic enzyme is Sumizyme MP, and Sumizyme MP is 1% with concentration of substrate ratio.
In preferred steps (3), pH value is 9.0. Under this pH value, it is possible to be conducive to Sumizyme MP to the hydrolysis of fish meal protein.
The temperature of preferred described enzymolysis is 60 DEG C, to facilitate enzymolysis process better.
Preparation method of the present invention, it is preferable that in step (4), enzymolysis solution is at 92 DEG C, the enzyme that goes out is lived 10 minutes, and then vacuum concentration under 0.1MPa, is drying to obtain.
Degreasing ocean of the present invention fish meal be deep-sea low value fish through the low-temperature defatted fish protein powder made, by those skilled in the art are grasped. Preferably protein content 55-75% in the fish meal of control degreasing ocean, moisture��10.0%, Residual oil��1.0%. Wherein, concrete preparation method can adopt disclosed in prior art multiple, as low value fish �� roughing becomes fish meal �� heating to add vapour modifier treatment �� make fish particulate material �� dipping extraction �� fat protein separation �� finished product fish meal. Described low value fish is such as fish etc.
2nd object of the present invention is the low value marine fish Gly-His-Lys protecting above-mentioned preparation method to obtain; this kind low value marine fish Gly-His-Lys possesses rich in nutrition content; the physiological functions such as absorption easy to digest, easy, enhancing immunity, can extensively for the additive of varieties of food items, feed etc.
Namely 3rd object of the present invention is that the above-mentioned low value marine fish Gly-His-Lys of protection is as the application in fodder additives; amino acid and low molecular peptide it is degraded into, therefore, after being prepared into fodder additives due to fish meal protein; the animal can be conducive to, it is to increase the digestibility of fish meal to absorb.
In addition, present invention also offers a kind of feed containing above-mentioned low value marine fish Gly-His-Lys, to improve nutritive value and the bioavailability of marine low-value fish. Wherein, described feed can continue to use various formula disclosed in prior art, and this is not particularly limited by the present invention.
It is an advantage of the current invention that: 1) adopt autoclaving fish meal raw material, be conducive to improving the solubleness of the protein of the tankage such as fish-bone, fish scale in fish meal; 2) adopting proteasome degradation method to produce bioactive peptide, reaction conditions is gentle, and enzymolysis process easily controls, environmentally friendly; 3) the nutrition composition of fish meal is improved,
The present invention makes full use of China's low value fish stock of existing abundant seawater, taking the low value fish meal of degreasing as raw material, protease hydrolyzed is utilized to prepare Gly-His-Lys, the functional performance of fish meal protein can be improved, improve the nutritive value of fish meal, for new approach is opened up in the exploitation of China's marine low-value fish protein.
Embodiment
Embodiment 1
Taking degreasing ocean fish meal as raw material, add water according to 1:10 material-water ratio and mix, at 121 DEG C, 1.05kg/cm2Boiling 25 minutes under pressure, take out and are cooled to 60 DEG C. Adjustment cooking liquor pH value to 9, taking Novozymes Company of Sumizyme MP Alcalase2.4L(Denmark) as enzymolysis enzyme, control enzyme-to-substrate concentration ratio is 1%, carries out enzymolysis at 60 DEG C, and remain on pH in enzymolysis process and stablize, after enzymolysis terminates, it is warming up to 92 DEG C, deactivation 10 minutes, filtrate is carried out vacuum concentration, thickening temperature 60 DEG C, concentrated pressure 0.1MPa, final drying and get final product.
After testing, in the low value marine fish Gly-His-Lys obtained by the present embodiment, oligopeptide content is 30.80%, is a kind of low value marine fish Gly-His-Lys of high quality.
Embodiment 2
Taking degreasing ocean fish meal as raw material, add water according to 1:5 material-water ratio and mix, at 115 DEG C, 0.75kg/cm2Boiling 20 minutes under pressure, take out and are cooled to 60 DEG C. Adjustment cooking liquor pH value to 7.0, taking flavor protease (Novozymes Company of Denmark) as enzymolysis enzyme, control enzyme-to-substrate concentration ratio is 0.5%, carries out enzymolysis at 45 DEG C, and remain on pH in enzymolysis process and stablize, after enzymolysis terminates, it is warming up to 90 DEG C, deactivation 10 minutes, filtrate is carried out vacuum concentration, thickening temperature 65 DEG C, concentrated pressure 0.12MPa, final drying and get final product.
After testing, in the low value marine fish Gly-His-Lys obtained by the present embodiment, oligopeptide content is 28.00%, is a kind of low value marine fish Gly-His-Lys of high quality.
Embodiment 3
Taking degreasing ocean fish meal as raw material, add water according to 1:12.5 material-water ratio and mix, at 130 DEG C, 1.75kg/cm2Boiling 30 minutes under pressure, take out and are cooled to 60 DEG C. Adjustment cooking liquor pH value to 9.5, taking Sumizyme MP (Wuxi Jie Nengke bio-engineering corporation) as enzymolysis enzyme, control enzyme-to-substrate concentration ratio is 2.5%, carries out enzymolysis at 70 DEG C, and remain on pH in enzymolysis process and stablize, after enzymolysis terminates, it is warming up to 95 DEG C, deactivation 10 minutes, filtrate is carried out vacuum concentration, thickening temperature 65 DEG C, concentrated pressure 0.12MPa, final drying and get final product.
After testing, in the low value marine fish Gly-His-Lys obtained by the present embodiment, oligopeptide content is 25.27%, is a kind of low value marine fish Gly-His-Lys of high quality.
Embodiment 4
Taking degreasing ocean fish meal as raw material, add water according to 1:7.5 material-water ratio and mix, at 125 DEG C, 1kg/cm2Boiling 24 minutes under pressure, take out and are cooled to 60 DEG C. Adjustment cooking liquor pH value to 8.5, first taking Sumizyme MP Alcalase2.4L as enzymolysis enzyme, control enzyme-to-substrate concentration ratio is 0.5%, at 55 DEG C, carry out enzymolysis 2h, then adjust ph to 7.0, add flavor protease, control enzyme-to-substrate concentration ratio is 0.5%, carries out enzymolysis 2h, after enzymolysis terminates at 55 DEG C, it is warming up to 95 DEG C, deactivation 10 minutes, carries out vacuum concentration by filtrate, thickening temperature 65 DEG C, concentrated pressure 0.12MPa, final drying and get final product.
After testing, in the low value marine fish Gly-His-Lys obtained by the present embodiment, oligopeptide content is 29.81%, is a kind of low value marine fish Gly-His-Lys of high quality.
Embodiment 5
Using preparation-obtained for embodiment 1 low value marine fish Gly-His-Lys as fodder additives, add by the addition of 8-10% in the daily feed of piglet, obtain the feed containing low value marine fish Gly-His-Lys.
Embodiment 6
Prepared for embodiment 5 pig starter feed containing low value marine fish Gly-His-Lys is fed piglet, promote the growth of piglet, reduce the usage quantity 1-10% of fish meal in feed, thus play the effect reducing feed cost, and improve the digestibility of piglet.
Claims (3)
1. the preparation method of one kind low value marine fish Gly-His-Lys, it is characterised in that: comprise the steps:
Taking degreasing ocean fish meal as raw material, add water according to 1:10 material-water ratio and mix, at 121 DEG C, 1.05kg/cm2Boiling 25 minutes under pressure, take out and are cooled to 60 DEG C;
Adjustment cooking liquor pH value to 9, taking Sumizyme MP Alcalase2.4L as enzymolysis enzyme, control enzyme-to-substrate concentration ratio is 1%, carries out enzymolysis at 60 DEG C, and remain on pH in enzymolysis process and stablize, after enzymolysis terminates, it is warming up to 92 DEG C, deactivation 10 minutes, filtrate is carried out vacuum concentration, thickening temperature 60 DEG C, concentrated pressure 0.1MPa, final drying and get final product;
Described degreasing ocean fish meal is deep-sea low value fish through the low-temperature defatted fish protein powder made, protein content 55-75%, moisture��10.0%, Residual oil��1.0%.
2. the low value marine fish Gly-His-Lys that preparation method described in claim 1 obtains.
3. contain the feed of low value marine fish Gly-His-Lys described in claim 2.
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| CN112352871A (en) * | 2020-09-13 | 2021-02-12 | 秦皇岛益尔生物科技有限公司 | Preparation technology of marine fish paste peptide and application of marine fish paste peptide in aquatic feed |
| CN112352870A (en) * | 2020-09-13 | 2021-02-12 | 秦皇岛益尔生物科技有限公司 | Preparation method of marine fish peptide suitable for piglet daily feed |
| CN112544786B (en) * | 2020-12-08 | 2023-10-20 | 舟山企惠科技发展有限公司 | Recovery method of low-value fish processing byproducts |
| CN112931701B (en) * | 2021-03-09 | 2022-01-07 | 清远希普生物科技有限公司 | Nutritional composition containing enzymolysis fish peptide powder and preparation method and application thereof |
| CN112956612B (en) * | 2021-04-12 | 2022-09-13 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Feed for improving disease resistance of stichopus japonicus and preparation method and application thereof |
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