CN105483195A - Method for preparing marine protein peptide by multi-step enzymolysis - Google Patents
Method for preparing marine protein peptide by multi-step enzymolysis Download PDFInfo
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- CN105483195A CN105483195A CN201511010948.6A CN201511010948A CN105483195A CN 105483195 A CN105483195 A CN 105483195A CN 201511010948 A CN201511010948 A CN 201511010948A CN 105483195 A CN105483195 A CN 105483195A
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Abstract
The invention relates to a method for preparing a marine protein peptide by multi-step enzymolysis, which comprises the following steps: by using marine low-value small rough fish as the raw material, grinding the raw material with a grinder, adding water, and homogenizing to obtain a homogenate solution; carrying out multi-step enzymolysis on the homogenate solution by animal protein hydrolase and trypsinase to obtain a secondary enzymolysis mixture; inactivating the enzyme, and centrifuging or filtering to obtain a supernate or filtrate; carrying out ultrafiltration/nanofiltration and concetration treatment on the supernate or filtrate to obtain a pasty marine protein peptide; and drying the pasty marine protein peptide to obtain the powdery marine protein peptide. By using the method, the final hydrolysis degree is up to 45% or so, the molecular weight of every enzymolysis product is 200-800Da, and thus, the enzymolysis products can be easily absorbed and utilized by organisms. The method is simple, convenient, green and environment-friendly, has the advantages of short production cycle and low cost, and is suitable for large-scale industrial production. The marine protein peptide can be used in health food or food, and can also be used in feed for livestock, poultry and marine lives to provide a novel protein source and additive.
Description
Technical field
The present invention relates to fishery products intensive processing field, particularly a kind of stepwise discretization prepares the method for sea protein peptide.
Background technology
Sea protein peptide is for main raw material with the flesh of fish of marine fishes, produce through enzymatic hydrolysis, can be dissolved by trichoroacetic acid(TCA), relative molecular mass at one group of low-molecular-weight oligo peptide of below 1000Da, there is easy absorption, absorb the features such as fast, low viscosity, good water solubility.
Modern nutrient research shows, the small peptide that molecular weight is less than 1000Da is very easily absorbed by the body utilization, and has stronger functionally active.It is reported, the Feed Energy that with the addition of sea protein peptide significantly improves the survival rate of animal, promotes feed conversion, realizes cultivation and significantly increases production and improves productivity.And the immunity function of little peptide can replace animal immunity potentiator to use, strengthen animal body autoimmunity.
At present, the preparation of protein peptide adopts single enzyme enzymolysis to obtain mostly.And single enzyme enzymolysis can only be hydrolyzed a kind of or class protein containing particular amino acid residue, sphere of action is little, and the degree of hydrolysis substrate is restricted, and enzymolysis time is longer, and peptide yield is lower.Therefore, the protein peptide of high degree of hydrolysis of how shortening the production cycle, improve small peptide yield within a short period of time, obtain is the large problem needing in preparation technology to solve.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, there is provided the two enzyme stepwise discretization of a kind of utilization easy and simple to handle, with short production cycle, that cost is low, product purity is high to prepare the method for sea protein peptide, improve the utilization ratio of sea low value little fish.
Technical problem to be solved by this invention is realized by following technical scheme.The present invention is a kind of method that stepwise discretization prepares sea protein peptide, is characterized in, its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:4-7 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 60-240U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 260-600U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; Be 45-55 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 6.5-8.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 1-4:1, under agitation carrying out enzyme digestion reaction 3-5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.01-0.04MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrates: described permeate is added infusion tank and carries out infusion concentration, obtain paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains lines marine protein peptide.
Animal proteolytic enzyme described in the present invention adopts any one animal proteolytic enzyme disclosed in commercially available or prior art, animal proteolytic enzyme is a kind of complex enzyme preparation special for animal proteinum hydrolysis, it is primarily of compositions such as protein incision enzyme, excision enzyme and food flavor enzymes, to cut off the peptide chain of protein interior and excision enzyme from centre by restriction endonuclease to cut off from the end of polypeptide chain and discharge amino acid, and food flavor enzyme plays optimization function to the bitter taste of hydrolysis and local flavor.
Stepwise discretization of the present invention is prepared in the method for sea protein peptide, and preferred technical scheme or technical characteristic are further:
1, in step (1): during homogenate, the mass ratio of raw material and water is 1:5.
2, in step (2): with pump by after described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 120-140U/g in described raw fish homogenate, obtain primary enzymolysis mixture.
3, in step (2): add trypsinase by enzyme concentration 340-360U/g in described primary enzymolysis mixture and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture.
4, in step (2): hydrolysis temperature is 50 DEG C, enzymolysis pH is 7.5, described animal proteolytic enzyme and tryptic enzymolysis time are than being 3:1, and enzymolysis total time is 3.5h.
5, in step (4): the working pressure of described ultrafiltration/nanofiltration membrane is 0.02MPa.
6, in step (5): described permeate is added infusion tank and carry out infusion concentration to moisture content at 45-55%.
7, in step (5): described permeate is added infusion tank and carry out infusion concentration to moisture content 50%.
8, in step (6): high-pressure spray-drying or lyophilize process are carried out to moisture content below 12% to described paste sea protein peptide.
9, in step (6): high-pressure spray-drying or lyophilize process are carried out to moisture content 10% to described paste sea protein peptide.
Compared with prior art, the inventive method adopts animal proteolytic enzyme and trypsinase stepwise discretization to prepare sea protein peptide, from different restriction enzyme sites, multidigit point enzymolysis is carried out to proteolytic enzyme, mutual coordination, overcome the shortcoming that single enzyme enzymolysis site is not enough, sphere of action is little, shorten the production cycle, improve degree of hydrolysis, protein peptide yield; Enzymolysis solution, through ultrafiltration/nanofiltration process, makes that the product purity of acquisition is high, quality is controlled.The inventive method is easy, with short production cycle, with low cost, environmental protection, is applicable to large-scale industrial production, can be used in protective foods or food, also can be livestock and poultry and aquatic feeds provides a kind of new protein sources and additive.
Accompanying drawing explanation
Fig. 1 is a kind of process flow sheet of the inventive method.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:4 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 60U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 260U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 45 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 6.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 1:1, under agitation carrying out enzyme digestion reaction 3h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.01MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate.
(5) infusion concentrates: described permeate is added infusion tank and carries out infusion concentration, obtain paste sea protein peptide.
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains lines marine protein peptide.
Embodiment 2, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:7 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 240U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 600U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 55 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 8.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 4:1, under agitation carrying out enzyme digestion reaction 5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.04MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate.
(5) infusion concentrates: described permeate is added infusion tank and carries out infusion concentration, obtain paste sea protein peptide.
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains lines marine protein peptide.
Embodiment 3, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:5 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 150U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 400U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 50 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 7.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 3:1, under agitation carrying out enzyme digestion reaction 3.5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.02MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate.
(5) infusion concentrates: described permeate is added infusion tank and carries out infusion concentration, obtain paste sea protein peptide.
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains lines marine protein peptide.
Embodiment 4, the stepwise discretization of embodiment 1-3 described in any one is prepared in the step (2) of the method for sea protein peptide: with pump by after described raw fish homogenate suction enzymatic vessel, in described raw fish homogenate, add animal proteolytic enzyme by enzyme concentration 120-140U/g, obtain primary enzymolysis mixture.All the other are identical.
Embodiment 5, the stepwise discretization of embodiment 1-3 described in any one is prepared in the step (2) of the method for sea protein peptide: in described primary enzymolysis mixture, add trypsinase by enzyme concentration 340-360U/g and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture.All the other are identical.
Embodiment 6, the stepwise discretization of embodiment 1-5 described in any one is prepared in the step (5) of the method for sea protein peptide: described permeate is added infusion tank and carry out infusion concentration to moisture content at 45-55%.
Embodiment 7, the stepwise discretization of embodiment 1-5 described in any one is prepared in the step (5) of the method for sea protein peptide: described permeate is added infusion tank and carry out infusion concentration to moisture content 50%.
Embodiment 8, the stepwise discretization of embodiment 1-7 described in any one is prepared in the step (6) of the method for sea protein peptide: carry out high-pressure spray-drying or lyophilize process to moisture content below 12% to described paste sea protein peptide.
Embodiment 9, the stepwise discretization of embodiment 1-7 described in any one is prepared in the step (6) of the method for sea protein peptide: carry out high-pressure spray-drying or lyophilize process to moisture content 10% to described paste sea protein peptide.
Embodiment 10, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:4 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 70U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 580-600U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 45 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 6.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 1:1, under agitation carrying out enzyme digestion reaction 3h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.01MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrate: described permeate is added infusion tank and carries out infusion concentration, obtain moisture content 45% paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains moisture content in 12% lines marine protein peptide.
Adopt the present embodiment method, final degree of hydrolysis reaches 44.8%.
Embodiment 11, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:7 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 230U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 370U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 55 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 8.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 4:1, under agitation carrying out enzyme digestion reaction 5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.04MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrate: described permeate is added infusion tank and carries out infusion concentration, obtain moisture content 55% paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains moisture content in 12% lines marine protein peptide;
Adopt the present embodiment method, final degree of hydrolysis reaches 45.3%.
Embodiment 12, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:6 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 180-200U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 260-280U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 55 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 8.0 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 2:1, under agitation carrying out enzyme digestion reaction 4h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.03MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrate: described permeate is added infusion tank and carries out infusion concentration, obtain moisture content 50% paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains moisture content in 8% lines marine protein peptide;
Adopt aforesaid method, final degree of hydrolysis reaches 44.5%.
Embodiment 13, with reference to Fig. 1, a kind of stepwise discretization prepares the method for sea protein peptide, and its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:5 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 130U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 350U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; It is 50 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 7.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 3:1, under agitation carrying out enzyme digestion reaction 3.5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.02MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrate: described permeate is added infusion tank and carries out infusion concentration, obtain moisture content 50% paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains moisture content in 10% lines marine protein peptide.
Adopt the present embodiment method, final degree of hydrolysis reaches 45.6%.
Claims (10)
1. stepwise discretization prepares a method for sea protein peptide, it is characterized in that, its step is as follows:
(1) Feedstock treating: sea low value little fish pulverizer that is fresh or that thaw is rubbed, after rubbing, raw material enters refiner and to add water homogenate than 1:4-7 by material quality, obtains raw fish homogenate;
(2) enzymolysis: with pump by described raw fish homogenate suction enzymatic vessel, add animal proteolytic enzyme by enzyme concentration 60-240U/g in described raw fish homogenate, after enzymolysis for some time, obtain primary enzymolysis mixture; In described primary enzymolysis mixture, add trypsinase by enzyme concentration 260-600U/g again and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture; Be 45-55 DEG C by temperature control device regulating and controlling temperature in enzymolysis process, pH is regulated to be 6.5-8.5 with sodium hydroxide solution or hydrochloric acid soln, described animal proteolytic enzyme and tryptic enzymolysis time, than being 1-4:1, under agitation carrying out enzyme digestion reaction 3-5h, obtain reacted liquid form mixt;
(3) go out enzyme: be warming up to more than 90 DEG C after enzymolysis terminates, and keep 10min, go out enzyme, and cooling, carries out centrifugal treating to the mixture after the enzyme that goes out, obtain supernatant liquor, or filtered by the mixture after the described enzyme that goes out, and obtains filtrate;
(4) ultrafiltration/nanofiltration: described supernatant liquor or filtrate are passed through Ultra filtration membrane device/nanofiltration membrane separation device, and working pressure controls at 0.01-0.04MPa, collects the oligopeptides of molecular weight between 200-800Da, obtains permeate;
(5) infusion concentrates: described permeate is added infusion tank and carries out infusion concentration, obtain paste sea protein peptide;
(6) dry: described paste sea protein peptide, through high-pressure spray-drying or lyophilize process, obtains lines marine protein peptide.
2. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (1): during homogenate, the mass ratio of raw material and water is 1:5.
3. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (2): with pump by after described raw fish homogenate suction enzymatic vessel, in described raw fish homogenate, add animal proteolytic enzyme by enzyme concentration 120-140U/g, obtain primary enzymolysis mixture.
4. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (2): add trypsinase by enzyme concentration 340-360U/g in described primary enzymolysis mixture and carry out enzyme digestion reaction, obtain secondary enzymolysis mixture.
5. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (2): hydrolysis temperature is 50 DEG C, enzymolysis pH is 7.5, described animal proteolytic enzyme and tryptic enzymolysis time are than being 3:1, and enzymolysis total time is 3.5h.
6. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (4): the working pressure of described ultrafiltration/nanofiltration membrane is 0.02MPa.
7. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (5): described permeate is added infusion tank and carry out infusion concentration to moisture content at 45-55%.
8. stepwise discretization according to claim 7 prepares the method for sea protein peptide, it is characterized in that, in step (5): described permeate is added infusion tank and carry out infusion concentration to moisture content 50%.
9. stepwise discretization according to claim 1 prepares the method for sea protein peptide, it is characterized in that, in step (6): carry out high-pressure spray-drying or lyophilize process to moisture content below 12% to described paste sea protein peptide.
10. stepwise discretization according to claim 9 prepares the method for sea protein peptide, it is characterized in that, in step (6): carry out high-pressure spray-drying or lyophilize process to moisture content 10% to described paste sea protein peptide.
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| CN107893098A (en) * | 2018-01-12 | 2018-04-10 | 广州欧普康特医食品有限公司 | A kind of ocean fish oligopeptide and its preparation method and application |
| CN111066894A (en) * | 2019-12-20 | 2020-04-28 | 福州海锐黎思生物科技有限责任公司 | Solid beverage of marine swimming bladder collagen and preparation method thereof |
| CN112352870A (en) * | 2020-09-13 | 2021-02-12 | 秦皇岛益尔生物科技有限公司 | Preparation method of marine fish peptide suitable for piglet daily feed |
| CN114807277A (en) * | 2022-04-12 | 2022-07-29 | 滨海宇美科技有限公司 | Method for preparing sea cucumber peptide by double enzymes step by step |
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| CN111066894A (en) * | 2019-12-20 | 2020-04-28 | 福州海锐黎思生物科技有限责任公司 | Solid beverage of marine swimming bladder collagen and preparation method thereof |
| CN112352870A (en) * | 2020-09-13 | 2021-02-12 | 秦皇岛益尔生物科技有限公司 | Preparation method of marine fish peptide suitable for piglet daily feed |
| CN114807277A (en) * | 2022-04-12 | 2022-07-29 | 滨海宇美科技有限公司 | Method for preparing sea cucumber peptide by double enzymes step by step |
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