CN110720549A - Preparation method of euphausia superba soluble pulp - Google Patents
Preparation method of euphausia superba soluble pulp Download PDFInfo
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- CN110720549A CN110720549A CN201910960548.3A CN201910960548A CN110720549A CN 110720549 A CN110720549 A CN 110720549A CN 201910960548 A CN201910960548 A CN 201910960548A CN 110720549 A CN110720549 A CN 110720549A
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- enzymolysis
- euphausia superba
- shrimp
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- soluble pulp
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- 241000239370 Euphausia superba Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241000238557 Decapoda Species 0.000 claims abstract description 30
- 239000004365 Protease Substances 0.000 claims abstract description 26
- 241000239366 Euphausiacea Species 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 22
- 108091005804 Peptidases Proteins 0.000 claims abstract description 17
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 17
- 150000001875 compounds Chemical class 0.000 claims abstract description 17
- 235000019419 proteases Nutrition 0.000 claims abstract description 17
- 108010007119 flavourzyme Proteins 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000013372 meat Nutrition 0.000 claims abstract description 11
- 108090000526 Papain Proteins 0.000 claims abstract description 9
- 229940055729 papain Drugs 0.000 claims abstract description 9
- 235000019834 papain Nutrition 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- 102000004142 Trypsin Human genes 0.000 claims description 5
- 108090000631 Trypsin Proteins 0.000 claims description 5
- 239000012588 trypsin Substances 0.000 claims description 5
- 238000000703 high-speed centrifugation Methods 0.000 claims description 2
- 229920000875 Dissolving pulp Polymers 0.000 abstract description 11
- 150000001413 amino acids Chemical class 0.000 abstract description 9
- 239000000047 product Substances 0.000 abstract description 7
- 239000002699 waste material Substances 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 235000019658 bitter taste Nutrition 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 4
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 235000012054 meals Nutrition 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000006227 byproduct Substances 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of aquatic product preparation, and provides a preparation method of euphausia superba dissolving pulp, aiming at solving the problems of low utilization rate of the traditional shrimp dissolving pulp, environmental pollution caused by direct discharge and resource waste, wherein the preparation method comprises the following steps of (1) mincing euphausia superba into meat paste, adding water, and stirring to obtain homogenate; (2) adding compound protease, and performing enzymolysis to obtain a first enzymolysis liquid; (3) adding flavourzyme, and carrying out enzymolysis to obtain a second enzymolysis liquid; (4) and oscillating and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp. The invention adopts a stepwise hydrolysis method, and the papain is beneficial to the enzymolysis of the components of the shrimp shell and the shrimp meat in the antarctic krill; the flavourzyme can remove bitter taste, improve mouthfeel and increase the content of amino acid in the shrimp dissolving pulp.
Description
Technical Field
The invention relates to the technical field of aquatic product preparation, and particularly relates to a preparation method of euphausia superba soluble pulp.
Background
The aquaculture industry develops at a high speed, and the shrimp meal yield cannot be synchronized, so that the demand of shrimp meal resources is short, and therefore, how to improve the utilization efficiency of the marine caught shrimps as feed products and reduce the use amount of the shrimp meal in artificial compound feed is an important research topic.
The antarctic krill shrimp soluble paste is a shrimp byproduct in the production process of shrimp meal, the components in the shrimp soluble paste are complex, a large amount of micro granular substances suspended in water, such as protein, peptide, amino acid and the like dissolved in water, biological amine, trimethylamine oxide, a small amount of oil components, trace elements and the like are also contained, and the biological active components have irreplaceable effects on the nutritional value of feed. However, most enterprises generally discharge the waste water directly, and the nutrient components in the waste water react with microorganisms and algae to cause malodor and red tide, so that the environment is polluted and the resource is wasted. For the above reasons, how to prepare a treated safe shrimp slurry from Antarctic krill has become an important research topic.
Chinese patent literature discloses a krill meal feed and a production process, and the publication number is CN 108850681A, the feed takes Antarctic krill meal as a raw material, has rich and balanced nutrition and good palatability, can provide sufficient nutrition for fish growth, is beneficial to improving the culture yield, and has low cost, convenient preparation and good economic benefit. However, the shrimp by-product Antarctic krill shrimp soluble pulp in the production process of the shrimp meal is not fully utilized, which causes resource waste.
Disclosure of Invention
The invention provides a preparation method of euphausia superba dissolving pulp with high safety and high resource utilization rate, aiming at overcoming the problems of low utilization rate of the traditional shrimp dissolving pulp, environmental pollution caused by direct discharge and resource waste.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of antarctic krill shrimp soluble pulp comprises the following steps:
(1) mincing Antarctic krill into minced meat, adding water with the weight being 2-4 times that of the minced meat, and uniformly stirring to obtain homogenate;
(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid;
(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid;
(4) and oscillating the second enzymolysis liquid under the water bath condition, and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp.
The hydrolysis of the shrimp dissolving pulp comprises an acid hydrolysis method, an alkali hydrolysis method, an enzymolysis method and the like, wherein the enzymolysis method destroys a peptide chain structure of protein in the shrimp dissolving pulp through protease to dissociate the protein into amino acid, the reaction condition is mild, the energy consumption can be effectively reduced, the cost is lower, and the waste liquid discharge is less, so the method is an ideal choice for treating the shrimp dissolving pulp. The invention can hydrolyze macromolecules such as protein, polypeptide and the like in the euphausia superba into amino acid which is easy to be absorbed by organisms by carrying out enzymolysis treatment on shrimp dissolving pulp which is a shrimp byproduct of the euphausia superba, so that the protein, the polypeptide and the like become raw materials suitable for storage and industrial development, the utilization rate of natural protein is improved, the resource waste is reduced, the discharge of the shrimp dissolving pulp is reduced, and the invention plays a great role in protecting the environment. The obtained hydrolysate contains free amino acids, and can be used as raw material for industrial production of liquid protein fertilizer. And the obtained hydrolysate can be subjected to deodorization treatment, so that the uncomfortable influence of the hydrolysate in the using process can be reduced.
Preferably, the compound protease is composed of the following components in parts by weight: 10-20 parts of papain and 30-50 parts of trypsin. The papain is beneficial to enzymolysis of components of the shrimp shell and the shrimp meat in the antarctic krill, the addition amount of the papain must be strictly controlled, and if the addition amount is too small, one-time enzymolysis is incomplete, and the subsequent enzymolysis effect is influenced; the addition amount is excessive, and unnecessary production cost is increased.
Preferably, in the step (2), the addition amount of the compound protease is 0.3-0.8% of the weight of the Antarctic krill.
Preferably, in the step (3), the addition amount of the flavourzyme is 2-3% of the weight of the antarctic krill. The flavourzyme can degrade the polypeptide into amino acid, the yield of the amino acid in the enzymolysis product is improved, meanwhile, the bitter peptide substances in the enzymolysis product in the first step are removed, the bitter taste of the enzymolysis product is removed, meanwhile, the addition amount of the flavourzyme must be strictly controlled, and the addition amount is too small, so that the secondary enzymolysis is incomplete, the yield of the amino acid is reduced, and the enzymolysis product has bitter taste; excessive addition results in waste of enzyme and unnecessary production costs.
Preferably, in the step (2), the pH value is controlled to be 8-9 and the temperature is controlled to be 45-50 ℃ in the enzymolysis process.
Preferably, in the step (3), the pH value is controlled to be 6-7 and the temperature is controlled to be 25-35 ℃ in the enzymolysis process.
Preferably, in the step (4), the water bath temperature is controlled to be 37-40 ℃, and the rotation speed of the high-speed centrifugation is controlled to be 18000-20000 r/min.
Therefore, the invention has the following beneficial effects: by adopting a step-by-step hydrolysis method, the papain is beneficial to the enzymolysis of the components of the shrimp shell and the shrimp meat in the antarctic krill; the flavourzyme can remove bitter taste, improve mouthfeel and increase the content of amino acid in the shrimp dissolving pulp.
Detailed Description
The technical solution of the present invention is further specifically described below by way of specific examples.
In the present invention, all the equipment and materials are commercially available or commonly used in the art, and the methods in the following examples are conventional in the art unless otherwise specified.
Example 1
(1) Mincing Antarctic krill into meat paste, adding 2 times of water by weight, and uniformly stirring to obtain homogenate;
(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid; the compound protease consists of 10g of papain and 50g of trypsin; the addition amount of the compound protease is 0.3 percent of the weight of the Antarctic krill; controlling the pH value to be 8 and the temperature to be 50 ℃ in the enzymolysis process;
(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid; the addition amount of the flavourzyme is 3% of the weight of the antarctic krill; controlling the pH value to be 7 and the temperature to be 35 ℃ in the enzymolysis process;
(4) and shaking the second enzymolysis liquid in a water bath at 37 ℃, and centrifuging at a high speed of 18000r/min to obtain supernatant, namely the euphausia superba soluble pulp.
Example 2
(1) Mincing Antarctic krill into meat paste, adding 4 times of water by weight, and uniformly stirring to obtain homogenate;
(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid; the compound protease consists of 20g of papain and 30g of trypsin; the addition amount of the compound protease is 0.8 percent of the weight of the euphausia superba; controlling the pH to be 9 and the temperature to be 45 ℃ in the enzymolysis process;
(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid; the addition amount of the flavourzyme is 2% of the weight of the antarctic krill; controlling the pH value to be 6 and the temperature to be 25 ℃ in the enzymolysis process;
(4) and oscillating the second enzymolysis liquid under the condition of water bath at 40 ℃, and centrifuging at a high speed of 20000r/min to obtain supernatant, namely the euphausia superba soluble pulp.
Example 3
(1) Mincing Antarctic krill into meat paste, adding 3 times of water by weight, and uniformly stirring to obtain homogenate;
(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid; the compound protease consists of 15g of papain and 35g of trypsin; the addition amount of the compound protease is 0.7 percent of the weight of the Antarctic krill; controlling the pH value to be 8 and the temperature to be 48 ℃ in the enzymolysis process;
(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid; the addition amount of the flavourzyme is 2.5 percent of the weight of the antarctic krill; controlling the pH value to be 6.5 and the temperature to be 30 ℃ in the enzymolysis process;
(4) shaking the second enzymolysis liquid under the condition of 38 ℃ water bath, and centrifuging at a high speed of 19000r/min to obtain supernatant, namely the antarctic krill shrimp soluble pulp.
The antarctic krill shrimp slurries prepared in examples 1-3 were tested and the results are shown in table 1:
TABLE 1 Performance results
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.
Claims (7)
1. A preparation method of antarctic krill shrimp soluble pulp is characterized by comprising the following steps:
(1) mincing Antarctic krill into minced meat, adding water with the weight being 2-4 times that of the minced meat, and uniformly stirring to obtain homogenate;
(2) adding compound protease into the homogenate for enzymolysis to obtain a first enzymolysis liquid;
(3) adding flavourzyme into the first enzymolysis liquid, and carrying out enzymolysis to obtain a second enzymolysis liquid;
(4) and oscillating the second enzymolysis liquid under the water bath condition, and centrifuging at a high speed to obtain supernatant, namely the euphausia superba soluble pulp.
2. The preparation method of the euphausia superba soluble pulp according to claim 1, wherein the compound protease is composed of the following components in parts by weight: 10-20 parts of papain and 30-50 parts of trypsin.
3. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (2), the addition amount of the compound protease is 0.3-0.8% of the weight of the euphausia superba.
4. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (3), the addition amount of the flavourzyme is 2-3% of the weight of the euphausia superba.
5. The method for preparing the euphausia superba soluble pulp according to claim 1, wherein in the step (2), the pH value during the enzymolysis is controlled to be 8-9, and the temperature is controlled to be 45-50 ℃.
6. The method for preparing the euphausia superba soluble pulp according to the claim 1, wherein in the step (3), the pH value is controlled to 6-7 and the temperature is controlled to 25-35 ℃ during the enzymolysis process.
7. The preparation method of the Antarctic krill shrimp soluble pulp according to claim 1, wherein in the step (4), the water bath temperature is controlled to be 37-40 ℃, and the rotation speed of the high-speed centrifugation is controlled to be 18000-20000 r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910960548.3A CN110720549A (en) | 2019-10-10 | 2019-10-10 | Preparation method of euphausia superba soluble pulp |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910960548.3A CN110720549A (en) | 2019-10-10 | 2019-10-10 | Preparation method of euphausia superba soluble pulp |
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| CN110720549A true CN110720549A (en) | 2020-01-24 |
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| CN201910960548.3A Pending CN110720549A (en) | 2019-10-10 | 2019-10-10 | Preparation method of euphausia superba soluble pulp |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112602851A (en) * | 2020-12-24 | 2021-04-06 | 日照职业技术学院 | Multistage utilization method of euphausia superba powder |
| CN114946999A (en) * | 2022-05-09 | 2022-08-30 | 广东海洋大学 | Method for preparing growth-promoting feed additive with high antibacterial property by using shrimp heads and/or krill |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392900A (en) * | 2013-07-25 | 2013-11-20 | 天津核生科技生物工程有限公司 | Method for preparing shrimp protein powder through shrimp hydrolysis |
| CN103834711A (en) * | 2014-03-04 | 2014-06-04 | 中国水产科学研究院东海水产研究所 | Preparation and application of protein hydrolysate of chilled Antarctic krill |
| CN108130353A (en) * | 2017-11-22 | 2018-06-08 | 浙江海洋大学 | A kind of method that oligopeptides powder is prepared using krill shell |
| CN110140898A (en) * | 2019-05-09 | 2019-08-20 | 浙江大学舟山海洋研究中心 | A kind of method that enzymatic hydrolysis prepares krill cream |
-
2019
- 2019-10-10 CN CN201910960548.3A patent/CN110720549A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392900A (en) * | 2013-07-25 | 2013-11-20 | 天津核生科技生物工程有限公司 | Method for preparing shrimp protein powder through shrimp hydrolysis |
| CN103834711A (en) * | 2014-03-04 | 2014-06-04 | 中国水产科学研究院东海水产研究所 | Preparation and application of protein hydrolysate of chilled Antarctic krill |
| CN108130353A (en) * | 2017-11-22 | 2018-06-08 | 浙江海洋大学 | A kind of method that oligopeptides powder is prepared using krill shell |
| CN110140898A (en) * | 2019-05-09 | 2019-08-20 | 浙江大学舟山海洋研究中心 | A kind of method that enzymatic hydrolysis prepares krill cream |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112602851A (en) * | 2020-12-24 | 2021-04-06 | 日照职业技术学院 | Multistage utilization method of euphausia superba powder |
| CN114946999A (en) * | 2022-05-09 | 2022-08-30 | 广东海洋大学 | Method for preparing growth-promoting feed additive with high antibacterial property by using shrimp heads and/or krill |
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Application publication date: 20200124 |