CN103789383A - Rice peptide production method - Google Patents
Rice peptide production method Download PDFInfo
- Publication number
- CN103789383A CN103789383A CN201410086971.2A CN201410086971A CN103789383A CN 103789383 A CN103789383 A CN 103789383A CN 201410086971 A CN201410086971 A CN 201410086971A CN 103789383 A CN103789383 A CN 103789383A
- Authority
- CN
- China
- Prior art keywords
- rice
- enzymolysis
- production method
- rice protein
- rice peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a rice peptide production method. The rice peptide production method comprises the steps of (A) mixing rice protein and a sodium sulfite solution to obtain rice protein slurry; (B) adding alkaline protease into the rice protein slurry to perform enzymolysis; (C) adding neutral protease, compound flavourzyme and papain for enzymolysis to obtain enzymatic hydrolysate; (D) performing enzyme deactivation treatment on the enzymatic hydrolysate; (E) filtering the enzymatic hydrolysate subjected to the enzyme deactivation and then performing freeze drying to obtain a high-purity rice peptide product. The rice peptide production method utilizes the synergistic effect of multiple enzymes, performs rice protein modification by adopting a multiple-process efficient separation method and is short in production period, high in yield and product purity and good in solubility through enzymolysis technology exploration and control.
Description
Technical field
The present invention relates to rice protein processing technique field, particularly the production method of rice peptide.
Background technology
Rice is the first agricultural-food of China and grain variety, and within 2008, China's rice yield accounts for 30% of global rice yield.Rice is the basic food that the mankind depend on for existence, and processing 100Kg paddy, can produce 60～65Kg rice, and all the other be 40Kg byproduct (rice husk, crack rice, rice bran and meter embryo), and these byproducts can obtain hundreds of high value added product after by intensive processing.
For a long time, China's rice is processed only in a kind of elementary process segment that meets grain ration rice demand, and the deep processing of byproduct is few, and resource utilization is lower.Along with the sharply increase of population, the exhausting fast of resource, rice is as the important existence food of the mankind, and the intensive processing of its byproduct is more and more subject to the attention of countries in the world.In edible rice processing, obtain crack rice, the process intensive processing such as rice bran, rice husk can obtain starchy carbohydrate, protein powder class and Rice pollard oil series products, the market of these products is in the growth stage, market value is high, and market outlook are good, is to make full use of rice resource effective means.
Rice protein powder is as one source protein resource relatively widely, and its trophic component is abundant, containing cholesterol, simultaneously compared with other plant source protein human body to rice protein without anaphylaxis.Especially more outstanding for its nutrition of special population such as patients with liver diseases, cardiovascular patient and baby and healthy advantage.But poorly soluble due to rice protein, molecular weight is large, the emulsifying property that it is lower, whipability and retentiveness have seriously limited the application of rice protein in foodstuffs industry.
At present more to the method for rice protein modification, mainly comprise chemistry, physics and enzyme-modified method.Chemic modified method easily produces chemical agent residue and has limited its application in the middle of food-processing.Physical modified method is due to its unstable to processing result control, and higher cost, is also unfavorable for the high-valued processing of low value material.Enzyme-modified method is due to specificity and the high efficiency of enzyme, has stable controlled working result, working (machining) efficiency is high and equipment cost is low feature, is applicable to being very much applied in rice protein modification processing.But existing enzyme modifying technology, some introducings other components carry out grafting coupling, reduced the purity of product protein, and complex process, process-cycle are long; What have has used acid-alkali treatment easily to introduce chemical residual in pretreatment stage; Although still its rice peptide yield is not high for the simple enzyme modifying technology of only applying having.
Summary of the invention
The object of the present invention is to provide that a kind of technique is simple, the process-cycle is short, without the production method of chemical residual and the high rice peptide of rice peptide yield.
According to an aspect of the present invention, provide the production method of rice peptide, its step is as follows:
A, the sodium sulfite solution that is 1～5% with concentration by rice protein powder mix in the ratio of solid-liquid ratio 1:5～1:15kg/L, and at 45～75 ℃, insulation 30～120min obtains rice protein slurry;
B, by the pH regulator to 7.5 of rice protein slurry～9.0, then to add Sumizyme MP be to continue to stir enzymolysis 0.5～2.5h for 7.5～9.0 times at pH, enzymolysis 0.5～2.5h is stirred in rear continuation;
Rice protein slurry pH to 6.5～7.5 of C, the processing of adjusting process Sumizyme MP, add neutral protease, compound protease and papoid, be to continue to stir enzymolysis 1～3h for 6.5～7.5 times at pH, enzymolysis 1～3h is stirred in rear continuation, obtains enzymolysis solution;
D, by the enzymolysis solution enzyme processing of going out;
E, the enzymolysis solution after sterilizing is filtered, then by nanofiltration membrane, filtrate is carried out to nanofiltration concentrated, after enzymolysis solution after nanofiltration is concentrated carry out high-temperature sterilization treatment, then obtain highly purified rice peptide product by lyophilize.
The present invention adopts plurality of enzymes synergy, and the method for Alternative high efficiency separation is carried out modification to rice protein, by the exploration to enzymolysis process and control, obtains with short production cyclely, and yield is high, and product purity is high, the rice peptide production method that solvability is good.
In some embodiments, the addition of step B neutral and alkali proteolytic enzyme is 0.1～5.0% of rice protein powder quality used.
In some embodiments, in step C, the addition of neutral protease, compound protease and papoid is respectively 0.1～5.0%, 0.1～5.0% and 0.5～3% of rice protein powder quality used.
In some embodiments, in step e nanofiltration membrane its to hold back relative molecular weight be 200～1000.
In some embodiments, the temperature of the enzyme processing of going out in step D is 85～90 ℃, and the time of the enzyme processing of going out is 20min.
The invention has the beneficial effects as follows:
1) the rice peptide product purity obtaining exceedes 95%, and average relative molecular mass is controlled at 500～1500 dalton.
2) utilize its yield of rice peptide that the rice peptide production method in the present invention is produced to exceed 50%, in prior art level advantageously position.
3) rice peptide production technique of the present invention is with short production cycle and produce hardly industrial pollution, environmental protection.
4) product solvability of the present invention is good, has kept the nonreactive characteristic of rice protein, and trophic component is comprehensive.Can add in infant formula, functional protein reconstituted food and some other protective foods as a kind of food ingredients of high-quality, greatly improve the added value of rice protein.
Embodiment
Starting material source:
Rice protein powder (crude protein content wt% >=80%, moisture wt%≤10%, ash content wt%≤5%, total number of bacterial colony cfu/g≤104): commercially available.
Sumizyme MP: letter Bioisystech Co., Ltd of Novi.
Neutral protease: Dong Henghua road, Nanning bio tech ltd.
Compound protease: Dong Henghua road, Nanning bio tech ltd.
Papoid: Pangbo Bioengineering Co Ltd, Nanning or Dong Henghua road, Nanning bio tech ltd.
Embodiment 1
Rice peptide production method, concrete steps are as follows:
A, take 100 order rice protein powder 5000g and drop in enzymatic vessels, add 2% sodium sulfite solution 25L, feed liquid is fully stirred evenly, the temperature of controlled enzymatic hydrolysis tank, at 45 ℃, is treated the follow-up continuation of insurance temperature of enzymatic vessel temperature-stable 30min.Obtain rice protein slurry.
B, the pH of rice protein slurry is controlled to 9.0 with 1mol/L sodium hydroxide solution.Add Sumizyme MP 5g to carry out enzymolysis, the pH reacting with the control of 1mol/L sodium hydroxide solution in enzymolysis process, 9.0, continues to stir enzymolysis 2.5h, then stops adding alkali, continues to stir enzymolysis 0.5h.
C, use 1mol/L hydrochloric acid soln regulate the pH to 7.2 through the rice protein slurry of Sumizyme MP processing, add neutral protease 25g, compound protease 25g and papoid 100g to carry out enzymolysis simultaneously, the pH reacting with the control of 1mol/L sodium hydroxide in enzymolysis process is 7.2, continue to stir enzymolysis 1h, then stop adding alkali, continue to stir enzymolysis 1h.Obtain enzymolysis solution.
D, the temperature of enzymatic vessel is risen to 90 ℃, keep the temperature of enzymolysis solution at 90 ℃ of lasting 20min, the enzyme processing of going out.
E, with vacuum filter by the enzymolysis solution filter 23 after sterilizing time, then with holding back relative molecular weight, to be that 200 nanofiltration membrane is carried out nanofiltration to filtrate concentrated, the enzymolysis solution after nanofiltration is concentrated, at 130 ℃, carries out high-temperature instantaneous sterilization processing under the condition of 5min.Then obtain highly purified rice peptide product by lyophilize.
Used production method is carried out to the mensuration of rice peptide yield, obtained product is carried out to solvability, protein molecular weight distribution, microbiological indicator and heavy metal index simultaneously and measure, the results are shown in Table 1.
Embodiment 2
Rice peptide production method, concrete steps are as follows:
A, take 300 order rice protein powder 5000g and drop in enzymatic vessels, add 1% sodium sulfite solution 50L, feed liquid is fully stirred evenly, the temperature of controlled enzymatic hydrolysis tank, at 65 ℃, is treated the follow-up continuation of insurance temperature of enzymatic vessel temperature-stable 75min.Obtain rice protein slurry.
B, after feed temperature is stable, the pH of rice protein slurry is controlled to 8.5 with 1mol/L sodium hydroxide solution.Add Sumizyme MP 125g to carry out enzymolysis, the pH reacting with the control of 1mol/L sodium hydroxide solution in enzymolysis process, 8.5, continues to stir enzymolysis 0.5h, then stops adding alkali, continues to stir enzymolysis 2.5h.
C, use 1mol/L hydrochloric acid soln regulate the rice protein slurry pH to 7.0 through Sumizyme MP processing, add neutral protease 5g, compound protease 5g and papoid 150g to carry out enzymolysis simultaneously, the pH reacting with the control of 1mol/L sodium hydroxide in enzymolysis process is 7.0, continue to stir enzymolysis 2h, then stop adding alkali, continue to stir enzymolysis 2h.Obtain enzymolysis solution.
D, the temperature of enzymatic vessel is risen to 90 ℃, keep the temperature of enzymolysis solution at 90 ℃ of lasting 20min, the enzyme processing of going out.
E, with vacuum filter by the enzymolysis solution filter 23 after sterilizing time, then with holding back relative molecular weight, to be that 1000 nanofiltration membrane is carried out nanofiltration to filtrate concentrated, the enzymolysis solution after nanofiltration is concentrated, at 135 ℃, carries out high-temperature instantaneous sterilization processing under the condition of 3min.Then obtain highly purified rice peptide product by lyophilize.
Used production method is carried out to the mensuration of rice peptide yield, obtained product is carried out to solvability, protein molecular weight distribution, microbiological indicator and heavy metal index simultaneously and measure, the results are shown in Table 1.
Embodiment 3
Rice peptide production method, concrete steps are as follows:
A, take 600 order rice protein powder 5000g and drop in enzymatic vessels, add 1% sodium sulfite solution 75L, feed liquid is fully stirred evenly, the temperature of controlled enzymatic hydrolysis tank, at 75 ℃, is treated the follow-up continuation of insurance temperature of enzymatic vessel temperature-stable 120min.Obtain rice protein slurry.
B, after feed temperature is stable, the pH of rice protein slurry is controlled to 7.5 with 2mol/L sodium hydroxide solution.Add Sumizyme MP 150g to carry out enzymolysis, the pH reacting with the control of 2mol/L sodium hydroxide solution in enzymolysis process, 8.5, continues to stir enzymolysis 1.5h, then stops adding alkali, continues to stir enzymolysis 1.5h.
C, use 2mol/L hydrochloric acid soln regulate the rice protein slurry pH to 6.5 through Sumizyme MP processing, add neutral protease 100g, compound protease 100g and papoid 25g to carry out enzymolysis simultaneously, the pH reacting with the control of 1mol/L sodium hydroxide in enzymolysis process is 7.5, continue to stir enzymolysis 3h, then stop adding alkali, continue to stir enzymolysis 3h.Obtain enzymolysis solution.
D, the temperature of enzymatic vessel is risen to 85 ℃, keep the temperature of enzymolysis solution at 85 ℃ of lasting 20min, the enzyme processing of going out.
E, with vacuum filter by the enzymolysis solution filter 23 after sterilizing time, then with holding back relative molecular weight, to be that 600 nanofiltration membrane is carried out nanofiltration to filtrate concentrated, the enzymolysis solution after nanofiltration is concentrated, at 140 ℃, carries out high-temperature instantaneous sterilization processing under the condition of 5min.Then obtain highly purified rice peptide product by lyophilize.
Embodiment 4
Rice peptide production method, concrete steps are as follows:
A, take 400 order rice protein powder 5000g and drop in enzymatic vessels, add 5% sodium sulfite solution 25L, feed liquid is fully stirred evenly, the temperature of controlled enzymatic hydrolysis tank, at 55 ℃, is treated the follow-up continuation of insurance temperature of enzymatic vessel temperature-stable 60min.Obtain rice protein slurry.
B, after feed temperature is stable, the pH of rice protein slurry is controlled to 8 with 1.5mol/L sodium hydroxide solution.Add Sumizyme MP 250g to carry out enzymolysis, the pH reacting with the control of 1.5mol/L sodium hydroxide solution in enzymolysis process, 8, continues to stir enzymolysis 1h, then stops adding alkali, continues to stir enzymolysis 1h.
C, use 2mol/L hydrochloric acid soln regulate the rice protein slurry pH to 7.5 through Sumizyme MP processing, add neutral protease 250g, compound protease 250g and papoid 50g to carry out enzymolysis simultaneously, the pH reacting with the control of 1mol/L sodium hydroxide in enzymolysis process is 7.5, continue to stir enzymolysis 1h, then stop adding alkali, continue to stir enzymolysis 1h.Obtain enzymolysis solution.
D, the temperature of enzymatic vessel is risen to 88 ℃, keep the temperature of enzymolysis solution at 88 ℃ of lasting 20min, the enzyme processing of going out.
E, with vacuum filter by the enzymolysis solution filter 23 after sterilizing time, then with holding back relative molecular weight, to be that 1000 nanofiltration membrane is carried out nanofiltration to filtrate concentrated, the enzymolysis solution after nanofiltration is concentrated, at 130 ℃, carries out high-temperature instantaneous sterilization processing under the condition of 6min.Then obtain highly purified rice peptide product by lyophilize.
Used production method is carried out to the mensuration of rice peptide yield, obtained product is carried out to solvability, protein molecular weight distribution, microbiological indicator and heavy metal index simultaneously and measure, the results are shown in Table 1.
Table 1 adopts the inventive method to produce rice peptide yield and product index measurement result
Reference:
1. Wang Zhi China, Wang Yimei, Cai Guangxia etc. the rapid assay methods [J] of rice peptide content. Food science, 2011,12 (32): 169～173
2. Chen Ji is prosperous, Sun Qingjie, summer Wenshui etc. the enzyme preparation technique of rice peptide and the research of characteristic [J] thereof. and Transactions of the Chinese Society of Agricultural Engineering, 2006 (22): 178～181
3. easily emerald green flat, Yao Huiyuan. the impact [J] of acid system deacylated tRNA amine on rice protein ultrastructure and relative molecular mass. Chinese grain and oil journal, 2007,2 (22): 1～4
High from the rice peptide product purity obtaining of table one, peptide yield is high, molecular weight is little, quality product is high.A kind of dietary supplements that are easy to digest and assimilate.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to the protection domain of invention.
Claims (5)
1. the production method of rice peptide, its step is as follows:
A, the sodium sulfite solution that is 1～5% with concentration by rice protein powder mix in the ratio of solid-liquid ratio 1:5～1:15kg/L, and at 45～75 ℃, insulation 30-120min obtains rice protein slurry;
B, by the pH regulator to 7.5 of rice protein slurry～9.0, then to add Sumizyme MP be to continue to stir enzymolysis 0.5～2.5h for 7.5～9.0 times at pH, enzymolysis 0.5～2.5h is stirred in rear continuation;
Rice protein slurry pH to 6.5～7.5 of C, the processing of adjusting process Sumizyme MP, add neutral protease, compound protease and papoid, be to continue to stir enzymolysis 1～3h for 6.5～7.5 times at pH, enzymolysis 1～3h is stirred in rear continuation, obtains enzymolysis solution;
D, by the enzymolysis solution enzyme processing of going out;
E, the enzymolysis solution after sterilizing is filtered, then by nanofiltration membrane, filtrate is carried out to nanofiltration concentrated, after enzymolysis solution after nanofiltration is concentrated carry out high-temperature sterilization treatment, then obtain rice peptide product by lyophilize.
2. the production method of rice peptide according to claim 1, is characterized in that: the addition of described step B neutral and alkali proteolytic enzyme is 0.1～5.0% of rice protein powder quality used.
3. the production method of rice peptide according to claim 1, is characterized in that: in described step C, the addition of neutral protease, compound protease and papoid is respectively 0.1～5.0%, 0.1～5.0% and 0.5～3% of rice protein powder quality used.
4. the production method of rice peptide according to claim 1, is characterized in that: in described step e nanofiltration membrane its to hold back relative molecular weight be 200～1000.
5. the production method of rice peptide according to claim 1, is characterized in that: the temperature of the enzyme processing of going out in described step D is 85～90 ℃, and the time of the enzyme processing of going out is 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410086971.2A CN103789383A (en) | 2014-03-11 | 2014-03-11 | Rice peptide production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410086971.2A CN103789383A (en) | 2014-03-11 | 2014-03-11 | Rice peptide production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103789383A true CN103789383A (en) | 2014-05-14 |
Family
ID=50665438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410086971.2A Pending CN103789383A (en) | 2014-03-11 | 2014-03-11 | Rice peptide production method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103789383A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146315A (en) * | 2015-07-02 | 2015-12-16 | 嘉兴恒杰生物制药有限公司 | Preparation process of low protein rice and rice peptide |
| CN107400690A (en) * | 2016-05-19 | 2017-11-28 | 大于生物科技有限公司 | Rice protein preparation method |
| CN108244328A (en) * | 2018-02-02 | 2018-07-06 | 安徽金太阳食品有限公司 | A kind of processing method of method of rice protein hydrolysate powder |
| TWI639383B (en) * | 2016-05-19 | 2018-11-01 | 倪秉豪 | Method for preparing rice protein |
| CN110029139A (en) * | 2019-01-31 | 2019-07-19 | 山东大树达孚特膳食品有限公司 | A kind of production technology of rice biologically active peptide |
| CN110115338A (en) * | 2019-05-28 | 2019-08-13 | 广东亨盛维嘉食品工业有限公司 | A kind of preparation method of subsensitivety nourishing rice flour for babies |
| CN115521962A (en) * | 2022-09-26 | 2022-12-27 | 福瑞施生物医药科技(深圳)有限公司 | Rice polypeptide composition and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974590A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Method for extracting rice peptides |
-
2014
- 2014-03-11 CN CN201410086971.2A patent/CN103789383A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974590A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Method for extracting rice peptides |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146315A (en) * | 2015-07-02 | 2015-12-16 | 嘉兴恒杰生物制药有限公司 | Preparation process of low protein rice and rice peptide |
| CN107400690A (en) * | 2016-05-19 | 2017-11-28 | 大于生物科技有限公司 | Rice protein preparation method |
| TWI639383B (en) * | 2016-05-19 | 2018-11-01 | 倪秉豪 | Method for preparing rice protein |
| CN107400690B (en) * | 2016-05-19 | 2021-02-05 | 倪秉豪 | Rice protein preparation method |
| CN108244328A (en) * | 2018-02-02 | 2018-07-06 | 安徽金太阳食品有限公司 | A kind of processing method of method of rice protein hydrolysate powder |
| CN110029139A (en) * | 2019-01-31 | 2019-07-19 | 山东大树达孚特膳食品有限公司 | A kind of production technology of rice biologically active peptide |
| CN110115338A (en) * | 2019-05-28 | 2019-08-13 | 广东亨盛维嘉食品工业有限公司 | A kind of preparation method of subsensitivety nourishing rice flour for babies |
| CN115521962A (en) * | 2022-09-26 | 2022-12-27 | 福瑞施生物医药科技(深圳)有限公司 | Rice polypeptide composition and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103789383A (en) | Rice peptide production method | |
| CN103689741B (en) | Method of preparing rice bran beverage employing enzymic method in cooperation with lactic acid fermentation | |
| CN102669423B (en) | Moringa extract as well as preparation method of moringa extract and moringa feed additive | |
| CN104263794A (en) | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method | |
| CN105167059B (en) | A kind of preparation method of multi-enzymatic hydrolysis pumpkin millet fermented beverage | |
| CN102994598B (en) | Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof | |
| CN101886106A (en) | Method for extracting collagen peptide from fish scales | |
| CN104996715B (en) | The technique that a kind of composite fermentation method prepares wheat germ polypeptide | |
| CN102138663A (en) | Method for processing fermented type nutritional fruit and vegetable powder rich in vitamin | |
| CN101297675B (en) | Industrial process for producing wheat peptide from glutelin powder by enzyme method | |
| CN110423691A (en) | A kind of method that amino acid zymotic fluid bacteria residue prepares mycoprotein medicinal extract | |
| CN106417891A (en) | Method for preparing gluten powder hydrolysate by utilizing enzymatic method | |
| CN108546724A (en) | High-purity oligoisomaltose and preparation method thereof | |
| CN103146782A (en) | Method for preparing glucose syrup by using rice bran as raw material | |
| CN108588053B (en) | Enzyme special for animal protein hydrolysis and preparation method thereof | |
| CN105483195A (en) | Method for preparing marine protein peptide by multi-step enzymolysis | |
| CN107400690A (en) | Rice protein preparation method | |
| CN101617847A (en) | Production method of potato peel healthcare vinegar drink and product thereof | |
| CN103478784A (en) | Method for preparing walnut kernel polypeptide through bacillus natto fermentation | |
| CN110897059A (en) | Method for preparing plant hydrolyzed protein beverage by using wheat germs | |
| CN102277403B (en) | Production technology of yellow wine lees proteins by enzymatic extraction | |
| CN107287258A (en) | A kind of preparation method of the resistant dextrin containing oligoisomaltose | |
| CN102550935A (en) | Method for extracting rice bran nutrients | |
| CN111789235A (en) | Sea cucumber compound enzymolysis method based on fucoidan and protease | |
| CN112794926A (en) | Extraction method and application of tamarind seed polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140514 |