CN108546724A - High-purity oligoisomaltose and preparation method thereof - Google Patents
High-purity oligoisomaltose and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of high-purity oligoisomaltoses and preparation method thereof,It is related to technical field of health care food,Starch fluid compound is first saccharified under beta amylase and Pullulanase effect by this method,Then glycosylated molecule structure bonding position is made to change using turning glycosides enzyme,It is converted to the oligoisomaltose with branched structure,The glucose in ingredient is then removed by membrane filtration,Glucose content is dropped to 8~10%,Oligoisomaltose content is set to be improved from 50% to 80% or more simultaneously,Then 1% or less can be down to by glucose content by being fermented again by active yeast,Oligomeric different wheat sugared content reaches 92% or more after purification,IG2+P+IG3 contents are up to 48% or more,This method is compared to existing oligoisomaltose preparation method,Advantage is more prominent on no matter in terms of product quality or production cost,Reach advanced international level,It is low with production cost,Production efficiency is high,It is suitble to the advantage of industrialized production.
Description
Technical field
The present invention relates to technical field of health care food, more particularly, to a kind of high-purity oligoisomaltose and its preparation side
Method.
Background technology
Oligoisomaltose (IMO) is also known as isomalto-oligosaccharide, isomaltulose, bifurcation oligose etc..It is starch
One kind of sugar, main component is between glucose molecule with α -1, the isomaltose (IG2) of 6 glycosidic bonds combination, panose (P), different wheat
Oligosaccharide more than bud trisaccharide (IG3) and tetrose (Gn).Currently, domestic and foreign scholars generally believe isomaltose, panose and different wheat
These three sugar of bud trisaccharide are to embody the functional main component of oligoisomaltose, and content height reflects the good of product quality
It is bad, also influence the price and application prospect of product.
Oligoisomaltose has sweet taste pure and sweet soft, and viscosity is relatively low, heat-resisting acidproof, and fermentability, moisture retention be not preferable
Etc. properties.In physiological function, oligoisomaltose has good Bifidobacterium proliferation activity, harmful bacteria can be inhibited to grow, and promotees
It is digested into intestines and stomach, helps enterotoxin row, promote absorption and the anti-caries tooth of the minerals such as calcium and magnesium, therefore be widely used in eating
In product, beverage and health products.
Domestic at present produced with market in oligoisomaltose is mainly 50 types (IMO50), i.e. oligoisomaltose content
50% or more, effective active object is relatively low, with the raising of living standards of the people and the enhancing of health care consciousness, now according to both at home and abroad
Market needs, tissue 90 types of research and development (IMO90), mainly using cornstarch as raw material, turns glycosides basis from raw sugar and enterprising passes through film
The advanced technologies such as filtering, fermentation, refined, concentration, the monosaccharide in IMO50 is detached, oligoisomaltose content is made to purify to
90% or more, wherein isomaltose IG2, panose, IG3 key components are up to 45% or more, different wheat more oligomeric than traditional 50 type IMO50
Bud sugar Bifidobacterium cultivation effect doubles, and product is enable to export to the countries and regions such as Canada.
But it is typically the method using membrane filtration in existing 90 type oligoisomaltose production process by oligomeric different wheat
Glucose in bud sugar crude product is down to 8% hereinafter, then directly extracting to obtain oligoisomaltose.Aforementioned production method
Have the shortcomings that apparent, firstly, since membrane filtration power demand is high, needs multistage pressure feed pump, power consumption is high, using membrane filtration
Glucose is down to 8% hereinafter, needing to consume a large amount of energy, and also to concentrate etc. after repeated multiple times membrane filtration by technology
Processing step will also consume a large amount of energy, and above method technique is cumbersome, low production efficiency, of high cost；Secondly, even with upper
Technique is stated, the content of glucose can only be at most reduced to 5% or so by membrane filter method, and 90 types obtained by the technique are oligomeric different
Maltose quality is simultaneously unstable, and the content of the oligoisomaltose after extraction can only be up to 90% or so, and technique control is careless slightly,
The content of the oligoisomaltose of production is difficult to the standard for ensureing to reach 90%, which is to cause to China Exports enterprise
Life, because once there is product quality phenomenon not up to standard, it is easy to lead to the return of goods of external importer, once such feelings
Condition generation will then bring export enterprise huge economic loss.
Therefore, research and develop a kind of preparation method of high-purity oligoisomaltose, this method have production cost it is low,
Production efficiency is high, production technology is controllable, the advantages of being suitble to industrialized production, while the content of oligoisomaltose obtained can be with
Guarantee reaches 90% or more preparation method, becomes very necessary and urgent.
In view of this, special propose the present invention.
Invention content
The first object of the present invention is to provide a kind of high-purity oligoisomaltose, the high-purity oligoisomaltose
Middle isomalto-oligosaccharide content >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.It can
Fully to meet the needs of 90 type oligoisomaltose of China Exports enterprise pair.
The second object of the present invention is to provide a kind of preparation method of the high-purity oligoisomaltose, this method phase
Prepare oligoisomaltose compared with simple embrane method or fermentation method, no matter in terms of product quality or production cost upper advantage is more
For protrusion, reach advanced international level, have production cost low, production efficiency is high, is suitble to the advantage of industrialized production.
A kind of high-purity oligoisomaltose provided by the invention, different malt is oligomeric in the high-purity oligoisomaltose
Sugared content >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.
A kind of preparation method according to above-mentioned high-purity oligoisomaltose provided by the invention, the preparation method include
Following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligomeric different wheat of oligoisomaltose content >=50%, IG2+P+IG3 content >=37%, glucose content >=28%
Bud sugar crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 8~10%, the oligoisomaltose crude product B of oligoisomaltose content >=80% are obtained；
(c) glucose fermentation：Active yeast is added in oligoisomaltose crude product B, is contained glucose by fermenting
Amount can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Further, the step of starch is carried out liquefaction processing by the step (a) is, by a concentration of 16.5~18.5 ° of Be '
Starch milk solution to adjust pH be 5.3~5.8, and α-amylase is added, after carrying out injection liquefaction, obtains liquefier；
Preferably, the injection liquefaction is injection liquefaction twice；
It is furthermore preferred that for the first time spray liquefied temperature be 100~110 DEG C, spray for the second time liquefied temperature 130~
140℃。
Further, the α-amylase is α-alpha-amylase；
Preferably, the additive amount of α-amylase is the 15~16% of the starch milk solution weight of 16~17 ° of Be '.
Further, the step of step (a) saccharification processing is that beta amylase and Propiram are added into liquefying starch
Enzyme is saccharified；
Preferably, by starch on dry basis weight, the dosage of 0.6-0.8 kg/ton of the dosage of beta amylase, Pullulanase is 0.1-
0.3 kg/ton；
It is furthermore preferred that pressing starch on dry basis weight, the dosage of 0.7 kg/ton of the dosage of beta amylase, Pullulanase is 0.2 thousand
G ton.
Further, the step (a) turn glycosides processing the step of be, when the dextrose equivalent of saccharified liquid be 35~40% when,
Addition turns glycosides enzyme and carries out after turning glycosides, obtains oligoisomaltose crude product A；
Preferably, the glycosides enzyme that turns is alpha-glucosaccharase transferase, and by starch on dry basis weight, the dosage for turning glycosides enzyme is 0.6-
0.8 kg/ton, preferably 0.7 kg/ton.
Further, step (a) liquefying starch be saccharified and turned glycosides processing treatment temperature be 55~65 DEG C.
Further, step (c) active yeast is high-temperature resistant and highly-active dry yeast bacterium, and the high temperature resistant is
The temperature of 30-38 DEG C of tolerance.
Further, ammonia nitrogen compound, the ammonia nitrogen chemical combination is added in the step (c) while active yeast is added
The dosage of object is 0.1-0.2 kg/ton；
Preferably, the ammonia nitrogen compound is ammonium chloride.
Further, the fermentation temperature of step (c) active yeast is 30-35 DEG C, fermentation time 18-36h；
Preferably, the fermentation temperature of step (c) active yeast is 31-34 DEG C, fermentation time 22-30h；
It is furthermore preferred that the fermentation temperature of step (c) active yeast is 33 DEG C, fermentation time is for 24 hours.
Compared with prior art, beneficial effects of the present invention are：
High-purity oligoisomaltose provided by the invention, isomalto-oligosaccharide contains in above-mentioned high-purity oligoisomaltose
Measure >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.China can fully be met to go out
The demand of 90 type oligoisomaltose of mouth trading enterprise pair.
The preparation method of high-purity oligoisomaltose provided by the invention, this method using amylase and Pullulanase,
Turn a variety of enzyme synergistic effects such as glycosides enzyme, starch is after liquefaction is handled, and be saccharified progress synchronous with glycosides is turned in saccharifying tank, starch fluid
Compound first produces maltose, maltotriose and polysaccharide, these wheats then generated under beta amylase and Pullulanase effect
Bud sugar makes molecular structure bonding position change with glycosides enzyme effect is turned, and is converted to the oligoisomaltose with branched structure, with
The glucose in ingredient is removed by membrane filtration afterwards, glucose content is dropped to 8~10%, while making oligoisomaltose content
Improved from 50% to 80% or more, then again by active yeast, by fermentation by glucose content can be down to 1% hereinafter,
IMO is up to 92% or more, IG2+P+IG3 contents up to 48% or more after purification for oligomeric different wheat sugar, and this method is compared to simple film
Method or fermentation method prepare oligoisomaltose, no matter in terms of product quality or production cost upper advantage is more prominent, reach
To advanced international level, have production cost low, production efficiency is high, is suitble to the advantage of industrialized production.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is a part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
According to an aspect of the present invention, a kind of high-purity oligoisomaltose, in the high-purity oligoisomaltose
Isomalto-oligosaccharide content >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.
High-purity oligoisomaltose provided by the invention, isomalto-oligosaccharide contains in above-mentioned high-purity oligoisomaltose
Measure >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.China can fully be met to go out
The demand of 90 type oligoisomaltose of mouth trading enterprise pair.
According to an aspect of the present invention, a kind of preparation method according to above-mentioned high-purity oligoisomaltose, the system
Preparation Method includes the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligomeric different wheat of oligoisomaltose content >=50%, IG2+P+IG3 content >=37%, glucose content >=28%
Bud sugar crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 8~10%, the oligoisomaltose crude product B of oligoisomaltose content >=80% are obtained；
(c) glucose fermentation：Active yeast is added in oligoisomaltose crude product B, is contained glucose by fermenting
Amount can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Preferably, the molecular weight of the filter membrane used in step (b) membrane filtration is 200.
The preparation method of high-purity oligoisomaltose provided by the invention, this method using amylase and Pullulanase,
Turn a variety of enzyme synergistic effects such as glycosides enzyme, starch is after liquefaction is handled, and be saccharified progress synchronous with glycosides is turned in saccharifying tank, starch fluid
Compound first produces maltose, maltotriose and polysaccharide, these wheats then generated under beta amylase and Pullulanase effect
Bud sugar makes molecular structure bonding position change with glycosides enzyme effect is turned, and is converted to the oligoisomaltose with branched structure, with
The glucose in ingredient is removed by membrane filtration afterwards, glucose content is dropped to 8~10%, while making oligoisomaltose content
Improved from 50% to 80% or more, then again by active yeast, by fermentation by glucose content can be down to 1% hereinafter,
IMO is up to 92% or more, IG2+P+IG3 contents up to 48% or more after purification for oligomeric different wheat sugar, and this method is compared to simple film
Method or fermentation method prepare oligoisomaltose, no matter in terms of product quality or production cost upper advantage is more prominent, reach
To advanced international level, have production cost low, production efficiency is high, is suitble to the advantage of industrialized production.
In the preferred embodiment of the present invention, the step of starch is carried out liquefaction processing by the step (a) is to incite somebody to action
It is 5.3~5.8 that the starch milk solution of a concentration of 16.5~18.5 ° of Be ', which adjusts pH, and α-amylase is added, after carrying out injection liquefaction,
Obtain liquefier；
Preferably, the injection liquefaction is injection liquefaction twice；
It is furthermore preferred that for the first time spray liquefied temperature be 100~110 DEG C, spray for the second time liquefied temperature 130~
140℃。
In above-mentioned preferred embodiment, the α-amylase is α-alpha-amylase；
Preferably, the additive amount of α-amylase is the 15~16% of the starch milk solution weight of 16~17 ° of Be '.
In the preferred embodiment of the present invention, the step of step (a) saccharification processing is, into liquefying starch
Beta amylase is added and Pullulanase is saccharified；
Preferably, by starch on dry basis weight, the dosage of 0.6-0.8 kg/ton of the dosage of beta amylase, Pullulanase is 0.1-
0.3 kg/ton；
It is furthermore preferred that pressing starch on dry basis weight, the dosage of 0.7 kg/ton of the dosage of beta amylase, Pullulanase is 0.2 thousand
G ton.
In the preferred embodiment of the present invention, the step (a) turn glycosides processing the step of be, when the Portugal of saccharified liquid
When grape sugar value is 35~40%, addition turns glycosides enzyme and carries out after turning glycosides, obtains oligoisomaltose crude product A；
Preferably, the glycosides enzyme that turns is alpha-glucosaccharase transferase, and by starch on dry basis weight, the dosage for turning glycosides enzyme is 0.6-
0.8 kg/ton, preferably 0.7 kg/ton.
In above-mentioned preferred embodiment, step (a) liquefying starch be saccharified and turned glycosides processing treatment temperature
It is 55~65 DEG C.
In the preferred embodiment of the present invention, step (c) active yeast is high temperature resistant wine brewing high activity
Cerevisin, the high temperature resistant are the temperature for being resistant to 30-38 DEG C.
As a preferred embodiment, above-mentioned high-temperature resistant and highly-active dry yeast bacterium is resistant to 30-38 DEG C of temperature, only
It ferments for glucose and part maltose, and to isomaltose azymic, glucose and maltose can be effectively eliminated quickly
To improve oligoisomaltose content.
In above-mentioned preferred embodiment, the dosage of step (c) active yeast is 0.6-0.8 kg/ton, into one
Preferably 0.7 kg/ton of step.
In the preferred embodiment of the present invention, ammonia is added while active yeast is added in the step (c)
The dosage of nitrogen compound, the ammonia nitrogen compound is 0.1-0.2 kg/ton；
As a preferred embodiment, ammonia nitrogen chemical combination is added in above-mentioned steps (c) while active yeast is added
Object can obviously reinforce the fermentation activity of active yeast, shorten fermentation time, and the same of yeast is being added through test of many times in we
When, by 0.1 kilogram of ammonium addition compound ammonium chloride per ton, it can at least improve 30% or more fermenting speed.
In above-mentioned preferred embodiment, the fermentation temperature of step (c) active yeast is 30-35 DEG C, when fermentation
Between be 18-36h；
Preferably, the fermentation temperature of step (c) active yeast is 31-34 DEG C, fermentation time 22-30h；
It is furthermore preferred that the fermentation temperature of step (c) active yeast is 33 DEG C, fermentation time is for 24 hours.
Technical scheme of the present invention is described further below in conjunction with embodiment and comparative example.
Embodiment 1
A kind of preparation method of high-purity oligoisomaltose, the preparation method comprises the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, it is thick to obtain oligoisomaltose content 50%, IG2+P+IG3 contents 37%, the oligoisomaltose of glucose content 28%
Product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 8%, the oligoisomaltose crude product B of oligoisomaltose content 80% are obtained；
(c) glucose fermentation：Active yeast and ammonium chloride are added in oligoisomaltose crude product B, it will by fermenting
Glucose content can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Embodiment 2
A kind of preparation method of high-purity oligoisomaltose, the preparation method comprises the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligoisomaltose of oligoisomaltose content 52%, IG2+P+IG3 contents 40%, glucose content >=28%
Crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 10%, the oligoisomaltose crude product B of oligoisomaltose content 82% are obtained；
(c) glucose fermentation：Active yeast and ammonium chloride are added in oligoisomaltose crude product B, it will by fermenting
Glucose content can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Embodiment 3
A kind of preparation method of high-purity oligoisomaltose, the preparation method comprises the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligoisomaltose of oligoisomaltose content 55%, IG2+P+IG3 contents 37%, glucose content >=28%
Crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 8%, the oligoisomaltose crude product B of oligoisomaltose content 85% are obtained；
(c) glucose fermentation：Active yeast and ammonium chloride are added in oligoisomaltose crude product B, it will by fermenting
Glucose content can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Embodiment 4
A kind of preparation method of high-purity oligoisomaltose, the preparation method comprises the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligoisomaltose of oligoisomaltose content 51%, IG2+P+IG3 contents 40%, glucose content >=28%
Crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 9%, the oligoisomaltose crude product B of oligoisomaltose content 83% are obtained；
(c) glucose fermentation：Active yeast and ammonium chloride are added in oligoisomaltose crude product B, it will by fermenting
Glucose content can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
Embodiment 5
A kind of preparation method of high-purity oligoisomaltose, the preparation method comprises the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified and is turned at glycosides successively
Reason, obtains the oligoisomaltose of oligoisomaltose content 52%, IG2+P+IG3 contents 42%, glucose content >=28%
Crude product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligomeric different malt
Sugar is enriched with, and glucose content 9%, the oligoisomaltose crude product B of oligoisomaltose content 80% are obtained；
(c) glucose fermentation：Active yeast and ammonium chloride are added in oligoisomaltose crude product B, it will by fermenting
Glucose content can be down to 1% hereinafter, obtaining high-purity oligoisomaltose after extracted.
In conclusion the preparation method of high-purity oligoisomaltose of the present invention, this method uses amylase and Propiram
Enzyme, a variety of enzymes synergistic effects such as turn glycosides enzyme, starch after liquefaction is handled, be saccharified in saccharifying tank it is synchronouss with glycosides is turned carry out, shallow lake
This first for producing maltose, maltotriose and polysaccharide under beta amylase and Pullulanase effect, then being generated of powder liquefied substance
A little maltose make molecular structure bonding position change with glycosides enzyme effect is turned, and are converted to the oligomeric different malt with branched structure
Sugar then removes the glucose in ingredient by membrane filtration, glucose content is dropped to 8~10%, while making oligomeric different malt
Sugared content is improved from 50% to 80% or more, and then again by active yeast, glucose content can be down to 1% by fermenting
Hereinafter, IMO is up to 92% or more, IG2+P+IG3 contents up to 48% or more after purification for oligomeric different wheat sugar, this method is compared to list
Pure embrane method or fermentation method prepare oligoisomaltose, and it is more prominent no matter advantage to be gone up in terms of product quality or production cost
Go out, reach advanced international level, have production cost low, production efficiency is high, is suitble to the advantage of industrialized production.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of high-purity oligoisomaltose, which is characterized in that isomalto-oligosaccharide in the high-purity oligoisomaltose
Content >=92%, IG2+P+IG3 content >=48%, glucose≤1%, solid content >=75%.
2. a kind of preparation method of the oligoisomaltose of high-purity according to claim 1, which is characterized in that the preparation side
Method includes the following steps：
(a) it pre-processes：Starch is subjected to liquefaction processing first, then liquefying starch is saccharified successively and turns glycosides processing, is obtained
It is thick to oligoisomaltose content >=50%, IG2+P+IG3 content >=37%, the oligoisomaltose of glucose content >=28%
Product A；
(b) membrane filtration：Oligoisomaltose crude product A is subjected to membrane filtration, removes part glucose, and to oligoisomaltose into
Row enrichment, it is 8~10% to obtain glucose content, the oligoisomaltose crude product B of oligoisomaltose content >=80%；
(c) glucose fermentation：Active yeast is added in oligoisomaltose crude product B, by fermenting glucose content energy
1% is down to hereinafter, obtaining high-purity oligoisomaltose after extracted.
3. the preparation method of high-purity oligoisomaltose according to claim 2, which is characterized in that the step (a)
The step of starch is carried out liquefaction processing is, is 5.3~5.8 by the starch milk solution adjusting pH of a concentration of 16~17 ° of Be ', and
α-amylase is added and obtains liquefier after carrying out injection liquefaction；
Preferably, the injection liquefaction is injection liquefaction twice；
It is furthermore preferred that it is 100~110 DEG C to spray liquefied temperature for the first time, 130~140 DEG C of liquefied temperature is sprayed for the second time.
4. the preparation method of high-purity oligoisomaltose according to claim 3, which is characterized in that the α-amylase is
α-alpha-amylase；
Preferably, the additive amount of α-amylase is the 15~16% of the starch milk solution weight of 16~17 ° of Be '.
5. the preparation method of high-purity oligoisomaltose according to claim 2, which is characterized in that the step (a)
The step of saccharification processing is that beta amylase is added into liquefying starch and Pullulanase is saccharified；
Preferably, by starch on dry basis weight, the dosage of 0.6-0.8 kg/ton of the dosage of beta amylase, Pullulanase is 0.1-0.3
Kg/ton；
It is furthermore preferred that press starch on dry basis weight, 0.7 kg/ton of the dosage of beta amylase, the dosage of Pullulanase is 0.2 kilogram/
Ton.
6. the preparation method of high-purity oligoisomaltose according to claim 2, which is characterized in that the step (a)
The step of turning glycosides processing is that, when the dextrose equivalent of saccharified liquid is 35~40%, addition turns glycosides enzyme and carries out after turning glycosides, obtains oligomeric
Isomaltose crude product A；
Preferably, the glycosides enzyme that turns is alpha-glucosaccharase transferase, and by starch on dry basis weight, the dosage for turning glycosides enzyme is 0.6-0.8 thousand
G ton, preferably 0.7 kg/ton.
7. the preparation method of high-purity oligoisomaltose according to claim 2, which is characterized in that the step (a)
Liquefying starch be saccharified and turned glycosides processing treatment temperature be 55~65 DEG C.
8. the preparation method of high-purity oligoisomaltose according to claim 2, which is characterized in that the step (c)
Active yeast is high-temperature resistant and highly-active dry yeast bacterium, and the high temperature resistant is the temperature for being resistant to 30-38 DEG C.
9. the preparation method of the high-purity oligoisomaltose according to claim 2 or 8, which is characterized in that the step
(c) dosage of active yeast is 0.6-0.8 kg/ton；
Preferably, ammonia nitrogen compound, the use of the ammonia nitrogen compound is added in the step (c) while active yeast is added
Amount is 0.1-0.2 kg/ton；
It is furthermore preferred that the ammonia nitrogen compound is ammonium chloride.
10. the preparation method of the high-purity oligoisomaltose according to claim 2 or 8, which is characterized in that the step
(c) fermentation temperature of active yeast is 30-35 DEG C, fermentation time 18-36h；
Preferably, the fermentation temperature of step (c) active yeast is 31-34 DEG C, fermentation time 22-30h；
It is furthermore preferred that the fermentation temperature of step (c) active yeast is 33 DEG C, fermentation time is for 24 hours.
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