CN101392281A - Method for preparing corn starch total sugar powder - Google Patents
Method for preparing corn starch total sugar powder Download PDFInfo
- Publication number
- CN101392281A CN101392281A CNA2008101948947A CN200810194894A CN101392281A CN 101392281 A CN101392281 A CN 101392281A CN A2008101948947 A CNA2008101948947 A CN A2008101948947A CN 200810194894 A CN200810194894 A CN 200810194894A CN 101392281 A CN101392281 A CN 101392281A
- Authority
- CN
- China
- Prior art keywords
- saccharification
- starch
- liquid glucose
- pullulanase
- glucoamylase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for preparing corn starch whole powered sugar, belonging to the technical field of bioengineering. The method adopts Alpha-amylase and diastatic enzyme (glucoamylase) to hydrolyze corn starch at different times; during the saccharification process, pullulanase is adopted for synergistic reaction so that the DE value of the final saccharification of the product can be enhanced to 98.80 percent. The method provides a new technical basis for using the corn starch to prepare high-quality whole powdered sugar. By the simultaneous use of a plurality of diastatic enzymes, the conversion rate of the product is enhanced and the production cycle is shortened, thus opening up wide application prospect for realizing the production of the high-quality whole powdered sugar in China.
Description
Technical field
A kind of preparation method of W-Gum total sugar powder belongs to technical field of bioengineering.
Background technology
Total sugar powder belongs to a kind of of starch sugar procut, is starch through after liquefaction, the saccharification, the Dian Fentang that obtains without the crystallization purging.But the transformation efficiency that a subject matter in process of production is exactly glucose in saccharifying is not high, and saccharification time is longer.After adopting the technology of enzyme process, the mashing transformation efficiency reaches more than 98%, and the DE value reaches more than 98%.Glucoamylase is a kind of excision enzyme, the decomposition of α-1,4 glycosidic link is very easy to, but for α-1,6 glycosidic link but decompose very slow, and all have α-1,6 glycosidic link in the W-Gum in 70% the amylopectin.Pullulanase is a side chain shallow lake phenolase, and α-1,6 glycosidic link that it props up the fork structure to hydrolysis has very strong effect.By utilizing the specificity of Pullulanase to α-1,6 glycosidic link, obtain a best manufacturing technology, improve the transformation efficiency of product and shorten the production cycle.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of W-Gum total sugar powder improves the transformation efficiency of product and shortens the production cycle.
Technical scheme of the present invention: a kind of preparation method of W-Gum total sugar powder, adopt α-Dian Fenmei, glucoamylase gradation that W-Gum is hydrolyzed and saccharification, in the process of saccharification, adopt Pullulanase to act synergistically, method is as follows:
(1) configuration corn starch milk: W-Gum is added the starch slurry that water is configured to weight percent 30%-40%;
(2) liquefaction: starch slurry is with HCl or NaCO
3Regulate powder slurry pH value to 6.2-6.5, add α-Dian Fenmei 30U/g starch, adopt the second spraying liquefaction process to liquefy, the steam ejection liquefaction temperature is 110 ℃ for the first time, 45min; The steam ejection liquefaction temperature is 130 ℃ for the second time, 15min; Become feminine gender to represent the terminal point that liquefies with the iodine color reaction; Temperature is elevated to 140 ℃ of deactivating enzyme 10min then; Cool to 60 ℃ then, get the starch liquefacation breast;
(3) saccharification: the starch liquefacation breast is regulated pH to 4.0-4.5 with HCl, temperature is controlled at 60 ± 1 ℃, add glucoamylase and Pullulanase and carry out saccharification react simultaneously, glucoamylase enzyme dosage 250u/g starch, Pullulanase consumption 0.13U/g starch, saccharification time control 60 hours when the DE value is almost constant, finishes saccharification;
(4) filter: the wilkinite that adds liquid glucose quality 1% in the liquid glucose after the saccharification filters with plate-and-frame filter press, removes the colloid that produces in the saccharifying, and then the diatomite filtration of adding liquid glucose quality 0.3% once, and the enzyme body in the liquid glucose is removed;
(5) decolouring: the liquid glucose after the filtration is warmed up to 80 ℃, adds the activated carbon of liquid glucose quality 0.2%-0.4%, and insulation 30min regulates pH to 6.0 with the sodium hydroxide of mass concentration 4%, and decolouring refilters once after finishing;
(6) spray crystallization: the filtrate after the decolouring is carried out spray crystallization with spray-dryer and is promptly got total sugar powder.
Beneficial effect of the present invention: the present invention adopts α-Dian Fenmei and saccharifying enzyme (glucoamylase) gradation that W-Gum is hydrolyzed, in the process of saccharification, adopt Pullulanase to act synergistically, make the final saccharification DE of product value bring up to 98.80%, provide new technological basis for W-Gum prepares high-quality total sugar powder production.Use simultaneously by multiple saccharifying enzyme, improved the transformation efficiency of product and shortened the production cycle, for application prospects has been opened up in the production that realizes China's high quality total sugar powder.
Embodiment
Embodiment 1: a kind of preparation of W-Gum total sugar powder:
W-Gum is configured to the starch slurry of 30%-40%.Regulate powder slurry pH value to 6.2-6.5 with HCl or NaCO3.Add α-Dian Fenmei 30U/g starch, adopt the second spraying liquefaction process to liquefy, the steam ejection liquefaction temperature is 110 ℃ for the first time, 45min, and the steam ejection liquefaction temperature is 130 ℃ for the second time, 15min becomes feminine gender to represent the terminal point that liquefies with the iodine color reaction.Temperature is elevated to 140 ℃ of deactivating enzyme 10min then.Cool to 60 ℃ then.Regulate pH to 4.0-4.5 with HCl, temperature is controlled at 60 ± 1 ℃, glucoamylase enzyme dosage 250u/g starch, Pullulanase consumption 0.13U/g starch.Saccharification time 60 hours when the DE value is almost constant, finishes saccharification.The wilkinite that liquid glucose after the saccharification adds liquid glucose quality 1% filters with plate-and-frame filter press, removes the colloid that produces in the saccharifying, and then the diatomite filtration of adding liquid glucose quality 0.3% once, and the enzyme body in the liquid glucose is removed.Liquid glucose after the filtration is warmed up to 80 ℃, adds the activated carbon of liquid glucose quality 0.2%-0.4% then, insulation 30min, and the sodium hydroxide with 4% is regulated pH to 6.0, and decolouring refilters once after finishing.Carry out spray crystallization with spray-dryer and promptly get total sugar powder.
Comparative examples: Pullulanase is to the synergy of saccharification
To the liquefaction breast of same liquefaction degree, carried out the mashing test contrast by contrasting only to add saccharifying enzyme (glucoamylase) and add Pullulanase and glucoamylase simultaneously.Measure a saccharification DE value every 12h behind the saccharification 12h, from changing conditions as can be seen, after in the saccharification system, adding Pullulanase, at identical saccharification time, inversion rate of glucose is higher than the system that only adds glucoamylase, when saccharification to 60h, have that saccharification DE value has reached 97.3% in the synergistic system.If do not add Pullulanase, the final saccharification DE value of saccharification 72h also has only 93.90%.
Claims (1)
1, a kind of preparation method of W-Gum total sugar powder is characterized in that adopting α-Dian Fenmei, glucoamylase gradation that W-Gum is hydrolyzed and saccharification, adopts Pullulanase to act synergistically in the process of saccharification, and method is as follows:
(1) configuration corn starch milk: W-Gum is added the starch slurry that water is configured to weight percent 30%-40%;
(2) liquefaction: starch slurry is with HCl or NaCO
3Regulate powder slurry pH value to 6.2-6.5, add α-Dian Fenmei 30U/g starch, adopt the second spraying liquefaction process to liquefy, the steam ejection liquefaction temperature is 110 ℃ for the first time, 45min; The steam ejection liquefaction temperature is 130 ℃ for the second time, 15min; Become feminine gender to represent the terminal point that liquefies with the iodine color reaction; Temperature is elevated to 140 ℃ of deactivating enzyme 10min then; Cool to 60 ℃ then, get the starch liquefacation breast;
(3) saccharification: the starch liquefacation breast is regulated pH to 4.0-4.5 with HCl, temperature is controlled at 60 ± 1 ℃, add glucoamylase and Pullulanase and carry out saccharification react simultaneously, glucoamylase enzyme dosage 250u/g starch, Pullulanase consumption 0.13U/g starch, saccharification time control 60 hours when the DE value is almost constant, finishes saccharification;
(4) filter: the wilkinite that adds liquid glucose quality 1% in the liquid glucose after the saccharification filters with plate-and-frame filter press, removes the colloid that produces in the saccharifying, and then the diatomite filtration of adding liquid glucose quality 0.3% once, and the enzyme body in the liquid glucose is removed;
(5) decolouring: the liquid glucose after the filtration is warmed up to 80 ℃, adds the activated carbon of liquid glucose quality 0.2%-0.4%, and insulation 30min regulates pH to 6.0 with the sodium hydroxide of mass concentration 4%, and decolouring refilters once after finishing;
(6) spray crystallization: the filtrate after the decolouring is carried out spray crystallization with spray-dryer and is promptly got total sugar powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101948947A CN101392281B (en) | 2008-10-23 | 2008-10-23 | Method for preparing corn starch total sugar powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101948947A CN101392281B (en) | 2008-10-23 | 2008-10-23 | Method for preparing corn starch total sugar powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101392281A true CN101392281A (en) | 2009-03-25 |
| CN101392281B CN101392281B (en) | 2011-06-22 |
Family
ID=40492792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101948947A Expired - Fee Related CN101392281B (en) | 2008-10-23 | 2008-10-23 | Method for preparing corn starch total sugar powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101392281B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812492A (en) * | 2010-04-27 | 2010-08-25 | 云南万芳生物技术有限公司 | Method for degrading cassava starch by pullulanase synergistic dual enzymatic method |
| CN101886145A (en) * | 2010-06-30 | 2010-11-17 | 潍坊盛泰药业有限公司 | High-yield glucose production method |
| CN103125805A (en) * | 2013-03-15 | 2013-06-05 | 甘肃乡草坊土特产品有限公司 | Potato crystal maltose and preparation method thereof |
| CN103266151A (en) * | 2013-05-24 | 2013-08-28 | 保龄宝生物股份有限公司 | Preparation method of moisturizing maltose powder |
| CN103981239A (en) * | 2014-05-13 | 2014-08-13 | 江南大学 | Method of improving saccharification product purity of high-concentration starch |
| CN110079571A (en) * | 2019-05-15 | 2019-08-02 | 纳来创硕湖北生物科技有限公司 | A kind of preparation process of high-quality fructose syrup |
| CN112481065A (en) * | 2020-11-26 | 2021-03-12 | 河南迪康生物科技股份有限公司 | Malt wine production process |
| WO2021082666A1 (en) * | 2019-10-31 | 2021-05-06 | 熊猫乳品集团股份有限公司 | Maltose syrup-containing condensed milk and preparation method therefor |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100548147C (en) * | 2006-12-29 | 2009-10-14 | 王德友 | Prepare the syrup dedicated method of bread with cornstarch |
| CN101117352B (en) * | 2007-08-29 | 2010-08-25 | 江南大学 | Method for producing high temperature stable slow-slaking amidon and uses thereof |
-
2008
- 2008-10-23 CN CN2008101948947A patent/CN101392281B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812492A (en) * | 2010-04-27 | 2010-08-25 | 云南万芳生物技术有限公司 | Method for degrading cassava starch by pullulanase synergistic dual enzymatic method |
| CN101812492B (en) * | 2010-04-27 | 2012-11-28 | 云南万芳生物技术有限公司 | Method for degrading cassava starch by pullulanase synergistic dual enzymatic method |
| CN101886145A (en) * | 2010-06-30 | 2010-11-17 | 潍坊盛泰药业有限公司 | High-yield glucose production method |
| CN103125805A (en) * | 2013-03-15 | 2013-06-05 | 甘肃乡草坊土特产品有限公司 | Potato crystal maltose and preparation method thereof |
| CN103266151A (en) * | 2013-05-24 | 2013-08-28 | 保龄宝生物股份有限公司 | Preparation method of moisturizing maltose powder |
| CN103266151B (en) * | 2013-05-24 | 2015-04-15 | 保龄宝生物股份有限公司 | Preparation method of moisturizing maltose powder |
| CN103981239A (en) * | 2014-05-13 | 2014-08-13 | 江南大学 | Method of improving saccharification product purity of high-concentration starch |
| CN103981239B (en) * | 2014-05-13 | 2016-09-07 | 江南大学 | A kind of method improving high concentration starch saccharification products purity |
| CN110079571A (en) * | 2019-05-15 | 2019-08-02 | 纳来创硕湖北生物科技有限公司 | A kind of preparation process of high-quality fructose syrup |
| WO2021082666A1 (en) * | 2019-10-31 | 2021-05-06 | 熊猫乳品集团股份有限公司 | Maltose syrup-containing condensed milk and preparation method therefor |
| CN112481065A (en) * | 2020-11-26 | 2021-03-12 | 河南迪康生物科技股份有限公司 | Malt wine production process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101392281B (en) | 2011-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101392281B (en) | Method for preparing corn starch total sugar powder | |
| CN103952452B (en) | A kind of preparation method of environment-friendly type high-purity oligoisomaltose | |
| CN101665843A (en) | Method for preparing barley maltsyrup by using wheat flour | |
| CN105368878A (en) | Method for producing alcohol by using manihot esculenta crantz as raw material | |
| CN102309011A (en) | Preparation method of dietary fiber of corn | |
| CN101278709A (en) | Method for extracting maltose powder, protein hydrolysate powder and cellulose using bran as raw material | |
| CN108546724A (en) | High-purity oligoisomaltose and preparation method thereof | |
| CN102603915A (en) | Method for extracting oat beta-glucan | |
| WO2009086781A1 (en) | Method for producing ethanol from root and tuber crops | |
| CN101693911B (en) | Liquor cleaning process for refining sugar from corns | |
| CN112048531B (en) | Maltooligosaccharide glucoside and preparation method and application thereof | |
| CN102465161A (en) | Method for producing high-purity maltose with double-enzyme method | |
| CN109527104A (en) | A kind of method that raw pulp method prepares soya-bean milk | |
| CN103911273B (en) | A kind of liquid fermentation embryo nelumbinis-germination rice vinegar and preparation technology thereof | |
| CN104341537A (en) | Method for arsenic removal and extraction of nutrient substances in sargassum fusiforme | |
| CN100532396C (en) | Process for preparing high purity pectin by using apple pomace | |
| CN102630887B (en) | Method for extracting dietary fibers from edible vegetables | |
| CN106674360A (en) | Purifying method of resistant dextrin | |
| CN104263628A (en) | Method for fermenting and preparing dendrobium huoshanness vinegar by taking processing excess material of rice and sugarcane as raw material | |
| CN101307278A (en) | Production process of draft rice wine | |
| CN102864180A (en) | Method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains | |
| CN104172387B (en) | A kind of method of producing jerusalem artichoke fructose beverage | |
| CN106755212A (en) | A kind of preparation method of maltitol | |
| KR20110056776A (en) | Vegetables or fruits enzymes and a producing mathod thereof | |
| CN102286553B (en) | Method for preparing lactic acid by furfural slag and Chinese honey locust slag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110622 Termination date: 20141023 |
|
| EXPY | Termination of patent right or utility model |