CN103981239A - Method of improving saccharification product purity of high-concentration starch - Google Patents
Method of improving saccharification product purity of high-concentration starch Download PDFInfo
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- CN103981239A CN103981239A CN201410201148.1A CN201410201148A CN103981239A CN 103981239 A CN103981239 A CN 103981239A CN 201410201148 A CN201410201148 A CN 201410201148A CN 103981239 A CN103981239 A CN 103981239A
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Abstract
The invention discloses a method of improving saccharification product purity of high-concentration starch and belongs to the technical field of starch saccharification. The starch is adopted as the main raw material. A starch liquefaction product is adopted as a substrate. A certain amount of pullulanase is added to replace a part of glucamylase, and participates the saccharification. High-concentration high-purity glucose syrup is finally obtained. The method increases the concentration of the substrate from 30% in industry to about 50%, and can reduce the evaporation energy consumption of glucose solutions in the later period of starch saccharification and can overcome the disadvantage that saccharification of high-concentration starch has many byproducts.
Description
Technical field
The present invention relates to a kind of method that improves high density mashing product purity, belong to starch refine dsugar technical field.
Background technology
β-amylose is taking starch as raw material, adopts acid system, acid-enzyme hydrolysis method or full-enzyme method to react the sugar products general name obtaining.Along with the raising of people's living standard, the demand of β-amylose is increasing.In β-amylose traditional processing technology, starch milk starting point concentration is controlled at 25%～35% conventionally, relatively low concentration can be brought the problems such as high water consumption, high energy consumption, in addition, in some amino acid, antibiotic fermentation, need to add more than 40% liquid glucose as carbon source, and the liquid glucose that traditional technology obtains need to concentrate and could use, be unfavorable for the control of cost, therefore, put forth effort on improvement β-amylose production technique very necessary.
Compared with traditional technology, improve the concentration of substrate, can effectively reduce water consumption, energy consumption and discharge of wastewater, can improve production intensity simultaneously, reduce production costs.But, in saccharifying, along with the raising of concentration of substrate, in product, glucose concn also can correspondingly improve, can there is complex reaction in the glucose of high density, generate the by products such as isomaltose, panose under the effect of glucoamylase, causes saccharification product purity to decline.Therefore, be necessary to improve the purity of high density mashing product.
Summary of the invention
The present invention is mainly to provide a kind of method that improves high density mashing product purity, is with Pullulanase Substitute For Partial glucoamylase, to improve significantly the purity of glucose in glucose syrup.
Described method preferably, taking the starch liquefacation product of DE value 5-25 as substrate, is prepared glucose under glucoamylase and Pullulanase katalysis.
Described starch liquefacation product preferably taking the starch of mass concentration 40%-60% through high temperature resistant α-amylaseliquefied to DE value as 5-25.
Described double-enzyme catalysis is prepared glucose and is preferably used hydrochloric acid adjust pH to 4.0-5.0, add glucoamylase and Pullulanase to carry out saccharification react simultaneously, the amount of glucoamylase is 20-100U/g starch (in butt), the amount of Pullulanase is 1-10ASPU/g starch (in butt), saccharification react temperature 40-60 DEG C, reaction times 12-72h.
The preferred 40U/g starch of consumption (in butt) of described glucoamylase.
The preferred 4ASPU/g starch of consumption (in butt) of described Pullulanase.
The present invention carrys out Substitute For Partial glucoamylase by adding Pullulanase, reduce the degree of high density mashing complex reaction, improve saccharification product purity, reduce the content of maltose, isomaltose, trisaccharide maltose, panose in product, can reduce the enzyme concentration of glucoamylase simultaneously.Processing condition provided by the invention, fully hydrolyzed starch can not bring again serious complex reaction, thereby improves glucose purity.
Embodiment
Detect final saccharification product by high performance liquid chromatography (HPLC).
Embodiment 1
Technological process:
50% (w/w, in starch butt) corn starch milk after high temperature resistant α-amylaseliquefied, obtain the starch liquefacation product of DE value 15 left and right, with hydrochloric acid adjust pH to 4.5, adding under the prerequisite of 4ASPU/g Pullulanase, add 30U/g glucoamylase, saccharification react temperature 60 C, reaction times 48h.Measure the impact of prozyme addition on mashing product composition, the results are shown in Table 1.
Embodiment 2
Technological process:
50% (w/w, in starch butt) corn starch milk after high temperature resistant α-amylaseliquefied, obtain the starch liquefacation product of DE value 15 left and right, with hydrochloric acid adjust pH to 4.5, adding under the prerequisite of 4ASPU/g Pullulanase, add 40U/g glucoamylase, saccharification react temperature 60 C, reaction times 48h.Measure the impact of prozyme addition on mashing product composition, the results are shown in Table 1.
Embodiment 3
Technological process:
50% (w/w, in starch butt) corn starch milk after high temperature resistant α-amylaseliquefied, obtain the starch liquefacation product of DE value 15 left and right, with hydrochloric acid adjust pH to 4.5, adding under the prerequisite of 4ASPU/g Pullulanase, add 50U/g glucoamylase, saccharification react temperature 60 C, reaction times 48h.Measure the impact of prozyme addition on mashing product composition, the results are shown in Table 1.
Comparative example 1
Technological process:
Carry out according to the method for embodiment 3, different is only to add glucoamylase, and does not add Pullulanase.The product purity that each embodiment obtains is in table 1.Can find out from embodiment 1-3 and comparative example 1, adopt the liquid glucose glucose purity that obtains of the inventive method compared with high, impurity is few, liquid glucose quality is better.In addition, the inventive method concentration of substrate is high, therefore can obtain the highly purified glucose syrup of high density.
Table 1
Note: this kind of material do not measured in-expression
Along with the raising of concentration of substrate, in saccharification product, glucose concn also can correspondingly improve, and the glucose of high density, under the effect of glucoamylase, complex reaction can occur, and generates the by products such as isomaltose, panose, causes saccharification product purity to decline.Pullulanase (Pullulanase, EC.3.2.1.41) is a kind of debranching factor, its energy hydrolyzing alpha-1, and 6-glycosidic link, but can not be hydrolyzed isomaltose, panose and only contain α-1, the polysaccharide of 6-glycosidic link.Carry out Substitute For Partial glucoamylase by adding Pullulanase, can reduce the degree of high density mashing complex reaction, improve saccharification product purity.
Although the present invention with preferred embodiment openly as above; but it is not in order to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, therefore protection scope of the present invention should be with being as the criterion that claims were defined.
Claims (6)
1. a method that improves high density mashing product purity, is characterized in that, is taking starch liquefacation product as substrate, adopts Pullulanase Substitute For Partial glucoamylase to participate in saccharification react, reduces the complex reaction of high concentration glucose.
2. method according to claim 1, is characterized in that, be starch liquefacation product taking DE value 5-25 as substrate, under glucoamylase and Pullulanase katalysis, prepare glucose.
3. method according to claim 2, it is characterized in that, mass concentration is that the starch milk of 40%-60% is after high temperature resistant α-amylaseliquefied, taking liquefied product as substrate, with hydrochloric acid adjust pH to 4.0-5.0, add glucoamylase and Pullulanase to carry out saccharification react, the amount of glucoamylase is in butt simultaneously: 20-100U/g starch, the amount of Pullulanase is in butt: 1-10ASPU/g starch.
4. method according to claim 3, is characterized in that, the consumption of described glucoamylase is preferably in butt: 40U/g starch.
5. method according to claim 3, is characterized in that, the consumption of described Pullulanase is preferably in butt: 4ASPU/g starch.
6. according to the arbitrary described method of claim 1-5, it is characterized in that saccharification react temperature 40-60 DEG C, reaction times 12-72h.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016087445A1 (en) * | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
| CN105963266A (en) * | 2016-05-09 | 2016-09-28 | 天津力能斯伯尔科技有限公司 | Glucose tablet and preparation method thereof |
| CN106119316A (en) * | 2016-06-23 | 2016-11-16 | 江南大学 | A kind of high concentration starch saccharification that improves produces the method for maltose yield in maltose syrup |
| CN106645125A (en) * | 2016-12-29 | 2017-05-10 | 南昌傲农生物科技有限公司 | Method for determining corn starch gelatinization degree |
| CN109694891A (en) * | 2019-02-01 | 2019-04-30 | 吉林中粮生化有限公司 | A method of utilizing high-concentration starch milk continuous production starch saccharification products |
| CN110004128A (en) * | 2019-03-18 | 2019-07-12 | 中粮集团有限公司 | Compounded saccharifying enzyme preparation and amylolytic method |
| CN112501226A (en) * | 2020-11-27 | 2021-03-16 | 广州双桥（重庆）有限公司 | Preparation method of syrup for medical lovastatin |
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| CN101392281A (en) * | 2008-10-23 | 2009-03-25 | 江南大学 | Method for preparing corn starch total sugar powder |
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| CN101392281A (en) * | 2008-10-23 | 2009-03-25 | 江南大学 | Method for preparing corn starch total sugar powder |
| CN102337316A (en) * | 2011-09-30 | 2012-02-01 | 广州双桥股份有限公司 | Process for producing starch sugar |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016087445A1 (en) * | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
| CN105963266A (en) * | 2016-05-09 | 2016-09-28 | 天津力能斯伯尔科技有限公司 | Glucose tablet and preparation method thereof |
| CN106119316A (en) * | 2016-06-23 | 2016-11-16 | 江南大学 | A kind of high concentration starch saccharification that improves produces the method for maltose yield in maltose syrup |
| CN106645125A (en) * | 2016-12-29 | 2017-05-10 | 南昌傲农生物科技有限公司 | Method for determining corn starch gelatinization degree |
| CN109694891A (en) * | 2019-02-01 | 2019-04-30 | 吉林中粮生化有限公司 | A method of utilizing high-concentration starch milk continuous production starch saccharification products |
| US10808270B2 (en) | 2019-02-01 | 2020-10-20 | Cofco Limited | Method for continuously producing starch saccharification products by using high-concentration starch milk |
| CN110004128A (en) * | 2019-03-18 | 2019-07-12 | 中粮集团有限公司 | Compounded saccharifying enzyme preparation and amylolytic method |
| CN112501226A (en) * | 2020-11-27 | 2021-03-16 | 广州双桥（重庆）有限公司 | Preparation method of syrup for medical lovastatin |
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| Publication number | Publication date |
|---|---|
| CN103981239B (en) | 2016-09-07 |
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