CN103981239B - A kind of method improving high concentration starch saccharification products purity - Google Patents
A kind of method improving high concentration starch saccharification products purity Download PDFInfo
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- CN103981239B CN103981239B CN201410201148.1A CN201410201148A CN103981239B CN 103981239 B CN103981239 B CN 103981239B CN 201410201148 A CN201410201148 A CN 201410201148A CN 103981239 B CN103981239 B CN 103981239B
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Abstract
The invention discloses a kind of method improving high concentration starch saccharification products purity, belong to starch sugar refining field.The present invention is with starch as primary raw material, and starch liquefacation product is substrate, adds a certain amount of Pullulanase Substitute For Partial glucoamylase and participates in saccharification reaction, finally gives a kind of highly purified glucose syrup of high concentration.Concentration of substrate is brought up to about 50% by industrial about 30% by the present invention, it is possible to reduce starch sugaring later stage liquid glucose evaporation energy consumption, and overcomes the high concentration starch sugaring more defect of accessory substance.
Description
Technical field
The present invention relates to a kind of method improving high concentration starch saccharification products purity, belong to starch sugar refining field.
Background technology
Starch sugar is with starch as raw material, uses acid system, acid-enzyme hydrolysis method or holoenzyme method to react the sugar products general name obtained.Along with
The raising of people's living standard, the demand of starch sugar is increasing.In starch sugar traditional processing technology, starch milk is the denseest
Degree is normally controlled in 25%～35%, and relatively low concentration can bring the problems such as high water consumption, high energy consumption, it addition, at some ammonia
Base acid, antibiotic fermentation in, need the liquid glucose adding more than 40% as carbon source, and the liquid glucose obtained by traditional handicraft needs
Concentrate and could use, be unfavorable for the control of cost, therefore, put forth effort on improvement starch sugar production technology the most necessary.
Compared with traditional handicraft, improve the concentration of substrate, can effectively drop low water consumption, energy consumption and discharge of wastewater, simultaneously energy
Carry high production intensity, reduce production cost.But, in saccharifying, along with the raising of concentration of substrate, in product, glucose is dense
Degree also can improve accordingly, and the glucose of high concentration can occur recombination reaction under the effect of glucoamylase, generates different Fructus Hordei Germinatus
The accessory substances such as sugar, panose, cause saccharification product purity to decline.Therefore, it is necessary to improve the purity of high concentration starch saccharification products.
Summary of the invention
The present invention mainly provides a kind of method improving high concentration starch saccharification products purity, is to substitute with Pullulanase
Part glucose amylase, to improve the purity of glucose in glucose syrup significantly.
Described method preferably with the starch liquefacation product of DE value 5-25 as substrate, is urged at glucoamylase and Pullulanase
Glucose is prepared under change effect.
Described starch liquefacation product preferably liquefies to DE through Thermostable α-Amylase with the starch of mass concentration 40%-60%
Value is 5-25.
Described double-enzyme catalysis is prepared glucose and is preferably adjusted pH value to 4.0-5.0 with hydrochloric acid, adds glucoamylase and general
Shandong orchid enzyme carries out saccharification reaction simultaneously, and the amount of glucoamylase is 20-100U/g starch (in terms of butt), the amount of Pullulanase
For 1-10ASPU/g starch (in terms of butt), saccharification reaction temperature 40-60 DEG C, reaction time 12-72h.
The consumption preferred 40U/g starch (in terms of butt) of described glucoamylase.
The consumption preferred 4ASPU/g starch (in terms of butt) of described Pullulanase.
The present invention carrys out Substitute For Partial glucoamylase by adding Pullulanase, reduces high concentration starch saccharification compound anti-
The degree answered, improves saccharification product purity, reduces maltose, isomaltose, maltotriose, the content of panose in product, simultaneously
The enzyme concentration of glucoamylase can be reduced.The process conditions that the present invention provides, can be fully hydrolyzed starch and will not bring again
Serious recombination reaction, thus improve glucose purity.
Detailed description of the invention
Final saccharification product is detected by high performance liquid chromatography (HPLC).
Embodiment 1
Technical process:
The corn starch milk of 50% (w/w, in terms of starch on dry basis), after Thermostable α-Amylase liquefies, obtains DE value 15 left
Right starch liquefacation product, adjusts pH value to 4.5 with hydrochloric acid, on the premise of adding 4ASPU/g Pullulanase, adds 30U/g Portugal
Grape saccharogenic amylase, saccharification reaction temperature 60 DEG C, reaction time 48h.Measure what starch saccharification products was formed by complex enzyme addition
Impact, the results are shown in Table 1.
Embodiment 2
Technical process:
The corn starch milk of 50% (w/w, in terms of starch on dry basis), after Thermostable α-Amylase liquefies, obtains DE value 15 left
Right starch liquefacation product, adjusts pH value to 4.5 with hydrochloric acid, on the premise of adding 4ASPU/g Pullulanase, adds 40U/g Portugal
Grape saccharogenic amylase, saccharification reaction temperature 60 DEG C, reaction time 48h.Measure what starch saccharification products was formed by complex enzyme addition
Impact, the results are shown in Table 1.
Embodiment 3
Technical process:
The corn starch milk of 50% (w/w, in terms of starch on dry basis), after Thermostable α-Amylase liquefies, obtains DE value 15 left
Right starch liquefacation product, adjusts pH value to 4.5 with hydrochloric acid, on the premise of adding 4ASPU/g Pullulanase, adds 50U/g Portugal
Grape saccharogenic amylase, saccharification reaction temperature 60 DEG C, reaction time 48h.Measure what starch saccharification products was formed by complex enzyme addition
Impact, the results are shown in Table 1.
Comparative example 1
Technical process:
Carry out according to the method for embodiment 3, the most only add glucoamylase, and without Pullulanase.Respectively
The product purity that embodiment obtains is shown in Table 1.From embodiment 1-3 and comparative example 1 it can be seen that use the sugar that the inventive method obtains
Liquid glucose purity compared with high, impurity is few, liquid glucose quality is preferable.It addition, the inventive method concentration of substrate is high, height therefore can be obtained
The highly purified glucose syrup of concentration.
Table 1
Note: represent and do not measure this kind of material
Along with the raising of concentration of substrate, in saccharification product, concentration of glucose also can improve accordingly, and the glucose of high concentration exists
Recombination reaction can occur under the effect of glucoamylase, generate the accessory substance such as isomaltose, panose, cause saccharification product purity
Decline.Pullulanase (Pullulanase, EC.3.2.1.41) is a kind of debranching enzyme, its energy hydrolyzing alpha-1,6-glycosidic bond, but not
Isomaltose, panose and the polysaccharide containing only α-1,6-glycosidic bond can be hydrolyzed.Substitute For Partial grape is taken by adding Pullulanase
Saccharogenic amylase, it is possible to reduce the degree of high concentration starch saccharification recombination reaction, improves saccharification product purity.
Although the present invention is open the most as above with preferred embodiment, but it is not limited to the present invention, any is familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclosing should be with being as the criterion that claims are defined.
Claims (4)
1. the method improving high concentration starch saccharification products purity, it is characterised in that be with the starch liquefacation of DE value 5-25
Product is substrate, uses Pullulanase Substitute For Partial glucoamylase to participate in saccharification reaction, reduces answering of high concentration glucose
Close reaction；Glucose is prepared under glucoamylase and Pullulanase catalytic action；Mass concentration is the starch of 40%-60%
Breast is after Thermostable α-Amylase liquefies, and with liquefaction products as substrate, adjusts pH value to 4.0-5.0 with hydrochloric acid, is simultaneously introduced grape
Saccharogenic amylase and Pullulanase carry out saccharification reaction, and the amount of glucoamylase is in terms of butt: 20-100U/g starch, Pu Lu
The amount of blue enzyme is in terms of butt: 1-10ASPU/g starch.
Method the most according to claim 1, it is characterised in that the consumption of described glucoamylase is preferably in terms of butt:
40U/g starch.
Method the most according to claim 1, it is characterised in that the consumption of described Pullulanase is preferably in terms of butt:
4ASPU/g starch.
4. according to the arbitrary described method of claim 1-3, it is characterised in that saccharification reaction temperature 40-60 DEG C, reaction time
12-72h。
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016087445A1 (en) * | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
| CN105963266A (en) * | 2016-05-09 | 2016-09-28 | 天津力能斯伯尔科技有限公司 | Glucose tablet and preparation method thereof |
| CN106119316B (en) * | 2016-06-23 | 2019-05-17 | 江南大学 | A method of it improving high concentration starch sugar metaplasia and produces maltose yield in malt syrup |
| CN106645125B (en) * | 2016-12-29 | 2019-09-03 | 南昌傲农生物科技有限公司 | A kind of measuring method of Gelatinization Degree of Maize Starch |
| CN109694891B (en) | 2019-02-01 | 2019-12-17 | 吉林中粮生化有限公司 | Method for continuously producing starch saccharification product by using high-concentration starch milk |
| CN110004128A (en) * | 2019-03-18 | 2019-07-12 | 中粮集团有限公司 | Compounded saccharifying enzyme preparation and amylolytic method |
| CN112501226A (en) * | 2020-11-27 | 2021-03-16 | 广州双桥（重庆）有限公司 | Preparation method of syrup for medical lovastatin |
Citations (2)
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| CN101392281A (en) * | 2008-10-23 | 2009-03-25 | 江南大学 | Method for preparing corn starch total sugar powder |
| CN102337316A (en) * | 2011-09-30 | 2012-02-01 | 广州双桥股份有限公司 | Process for producing starch sugar |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392281A (en) * | 2008-10-23 | 2009-03-25 | 江南大学 | Method for preparing corn starch total sugar powder |
| CN102337316A (en) * | 2011-09-30 | 2012-02-01 | 广州双桥股份有限公司 | Process for producing starch sugar |
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| 普鲁兰酶在生产玉米淀粉全糖粉中的应用与研究;张银志;《安徽农业科技》;20091231;第37卷(第1期);全文 * |
| 曹新阳等.脱支酶在糖化过程中的应用.《酿酒》.2003,第30卷(第4期), * |
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Address after: 214122 Jiangsu city of Wuxi Province District Liangxi No. 898 South Road 7 layer beam Creek area of food science and Technology Park Patentee after: Jiangnan University Address before: 1800 No. 214122 Jiangsu city of Wuxi Province Li Lake Avenue Patentee before: Jiangnan University |