CN101278709A - Method for extracting maltose powder, protein hydrolysate powder and cellulose using bran as raw material - Google Patents
Method for extracting maltose powder, protein hydrolysate powder and cellulose using bran as raw material Download PDFInfo
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- CN101278709A CN101278709A CNA2008100160486A CN200810016048A CN101278709A CN 101278709 A CN101278709 A CN 101278709A CN A2008100160486 A CNA2008100160486 A CN A2008100160486A CN 200810016048 A CN200810016048 A CN 200810016048A CN 101278709 A CN101278709 A CN 101278709A
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Abstract
A method which is used for extracting maltose flour, zitan flour and cellulose by taking wheat bran as raw material belongs to the technical field of the comprehensive development of farm and sideline products and functional food additive. The technical points of the method are that ordinary wheat bran is stewed and pasted by adding water; high-temperature-resistant amylase and hemicellulase are added for enzymolysis; enzymolysis liquid is filtered to be processed for the enzymolysis, so as to obtain maltose liquid; compound proteinase is added into filter residues for the enzymolysis, the enzymolysis liquid is filtered again, filtrate is zitan liquid, and the filter residues is cleaned by water to obtain the crude product of cellulose with high purity. The maltose liquid and the zitan liquid are processed by separation, purification, concentration and spray-drying to obtain the maltose flour and the zitan flour. The maltose flour, the zitan flour and the cellulose are widely applied to the food service industry. The method of the invention extracts and uses three main component in the wheat bran synchronously for the first time, which really realize the effective increment and utilization of the wheat bran, and what is more important is that the technique of the invention does not discharge any pollutant during the actual production process.
Description
Technical field:
The present invention relates to a kind of is that raw material extracts maltitol powder, protein hydrolysate powder and cellulosic method with wheat bran, belongs to agricultural byproducts comprehensive exploitation and functional food additives technical field.
Background technology:
Wheat bran is the main accessory substance of wheat processing, contain starch, 10-15% albumen, 8-9% crude fibre more than 40%, the output of the annual wheat bran of China is about about 2,000 ten thousand tons, it is a bigger byproduct resource, though it is that feedstock production is extracted different products such as pentosan, oxalate oxidase, multifunctional diet fibre, feruloylated oligosaccharides, antifreeze protein with wheat bran that part enterprise is arranged, obtain certain economic and social benefit, but still failed effectively to solve the comprehensive utilization of blowdown problem and resource.If that can realize extracts simultaneously to the starch in the wheat bran, albumen, dietary fiber, not only resource is fully utilized, and pollution problem has obtained basic solution, this will have crucial meaning concerning the enterprises engaged in processing agricultural products of China, also really reached the requirement of " energy-saving and emission-reduction " of national proposition.
Summary of the invention:
The purpose of this invention is to provide a kind of is that raw material extracts maltitol powder, protein hydrolysate powder and cellulosic method with wheat bran, to wheat bran main component starch, albumen and the comprehensive extraction and application of cellulose, the real realization to this huge resources effective increment utilization of wheat bran.
The objective of the invention is to take following steps to realize:
A. the water that wheat bran is added 4-9 times of quality, be warming up to 90 ℃-100 ℃, be incubated 20-30 minute and carry out gelatinization, AMS and hemicellulase enzymolysis are added in gelatinization back well, wherein the AMS addition is the 0.0015%-0.008% of wheat bran raw material quality, the hemicellulase addition is the 0.0015%-0.009% of wheat bran raw material quality, and enzymolysis liquid filters, and filtrate is the Fructus Hordei Germinatus liquid glucose; Gained filtrate concentrates again through separation and purification, is drying to obtain maltitol powder;
B. the filter residue that last step enzymolysis liquid filtration obtains adds and is equivalent to wheat bran raw material quality 5-8 water doubly, add the compound protease that is equivalent to wheat bran raw material quality 0.008%, carried out enzymolysis process in 4-5 hour 40 ℃-75 ℃ insulations, enzymolysis liquid filters, and filtrate is hydrolyzed protein liquid; Filtrate is through purifying, concentrate, the dry protein hydrolysate powder that gets;
C. go up the step filter residue through decolouring, washing, centrifugal dehydration, the dry cellulose that gets.
Wherein: a adds AMS earlier in the step, and regulatory enzyme enzymatic hydrolysis system pH value keeps 50 ℃-75 ℃ of hydrolysis temperatures, temperature retention time 60-80 minute to 5.5-7; Cool to 40 ℃-55 ℃ then, with hemicellulase, regulatory enzyme enzymatic hydrolysis system pH value is incubated 3-4 hour to 4.5-6.5.
In addition: during the compound protein enzymolysis, add the papain that is equivalent to wheat bran raw material quality 0.0015%-0.007% earlier in the b step, be warmed up to 40 ℃-75 ℃, regulatory enzyme enzymatic hydrolysis system pH value is incubated 2-3 hour to 4.5-7.5; Be incubated 40 ℃-75 ℃ then, sovolin in wheat bran raw material quality 0.0015%-0.007% De ?enzyme, be incubated 2-3 hour.
Maltitol powder: maltose is human the earliest as the sugar of sweetener, is widely used in the food industry such as fermentation, beverage, can, candy, cake and prevents that as temperature regulato, crystallization agent, swelling agent etc. from having effect preferably; Dietary fiber: be a kind of can not be by the carbohydrate of human consumption, the digestive system of maintenance health is arranged, strengthen immune system, reduce cholesterol and effects such as hypertension, reduction insulin and triglyceride disease, dietary fiber also is the indispensable material of human body, modern nutrition is referred to as " the seventh-largest nutrient ", and brainstrust is consistent to be thought: fiber food will be 21 century to dominate one of food; Protein hydrolysate powder: can be used as the proteinaceous nutrient hardening agent, can remedy the deficiency of protein in the meals, can replace egg, be used for cake, bread, and have purposes such as the food of preventing is aging clearly as blowing agent.This three big product all is widely used in the food service industry, and more and more important, and demand is increasing.
The invention has the beneficial effects as follows: (1) is owing to adopted AMS and hemicellulase complex enzyme that wheat bran is made up enzymolysis, basically the starch in the wheat bran is become maltose fully, also hemicellulose has been resolved into maltose, increase maltose concentration, changed the color and luster of Fructus Hordei Germinatus liquid glucose, extracted the high-quality dietary fiber for the back operation simultaneously and laid extraordinary basis; (2) owing to adopted the complex enzyme processing, the cellulose mouthfeel of production, color and luster all are better than chemical method greatly, do not have peculiar smell; (3) comprehensive utilization ratio of wheat bran can reach 100%, and the raw material availability height is good in economic efficiency, without sewage discharge.
The specific embodiment:
Embodiment 1
Take by weighing wheat bran 600g, add water 4kg, be warmed up to 90 ℃, gelatinization 20 minutes cools to 75 ℃, adds AMS 3g, and adjust pH to 6.0 stirs, and is incubated 1 hour; Cool to 45 ℃ again, add cellulose complex enzyme 3g, adjust pH to 5.0 stirs insulation 3-4 hour, filters, and collects filtrate 3500ml, and concentration 8.5% is separated and purified, and concentrate drying gets maltitol powder 294g.Filter residue adds 3.5kg water, is warming up to 60 ℃, adds protease 2.4g, and adjust pH to 6.5 stirs insulation 2 hours, be cooled to 45 ℃ of ， Jia ?enzyme 2.5g, stir insulation 2.5 hours.Filtration, filtrate is protein liquid, collects filtrate 2500ml, and concentration is 3%, separates and purifies, and concentrates, and drying gets protein hydrolysate powder 75g, and protein content 94% after testing.Filter residue adds 800g water, add 50g hydrogen peroxide and 10ml hypochlorous acid combination decolouring, washing, centrifugal dehydration again after, dry must cellulose 102g.
Embodiment 2
Take by weighing wheat bran 600g, add water 5000g, be warmed up to 95 ℃, gelatinization 30 minutes cools to 60 ℃, adds AMS 4g, and adjust pH to 6.3 stirs, and is incubated 1 hour; Cool to 48 ℃ again, add cellulose complex enzyme 2.5g, adjust pH to 5.2 stirs insulation 3.5 hours, filters, and collects filtrate 4500ml, and concentration 6.3% is separated and purified, and concentrate drying gets maltitol powder 283.5g.Filter residue adds 4200g water, is warming up to 64 ℃, adds protease 2.2g, and adjust pH to 6.0 stirs insulation 2 hours, be cooled to 48 ℃ of ， Jia ?enzyme 2.9g, stir insulation 2 hours.Filtration, filtrate is protein liquid, collects filtrate 3000ml, and concentration is 2.4%, separates and purifies, and concentrates, and drying gets protein hydrolysate powder 72g, and protein content 90% after testing.Filter residue adds 900g water, adds 45g hydrogen peroxide and 15ml hypochlorous acid combination decolouring again, washing, and after the centrifugal dehydration, the dry cellulose 100g that gets.
Embodiment 3
Take by weighing wheat bran 600g, add water 5400g, be warmed up to 100 ℃, gelatinization 30 minutes cools to 65 ℃, adds AMS 5g, and adjust pH to 6.8 stirs, and is incubated 1.2 hours; Cool to 50 ℃ again, add cellulase 4g, adjust pH to 6.5 stirs insulation 4 hours, filters, and collects filtrate 5000ml, and concentration 5.9% is separated and purified, and concentrate drying gets maltitol powder 295g.Filter residue adds 4200g water, is warming up to 55 ℃, adds protease 2.9g, and adjust pH to 6.8 stirs insulation 2 hours, be cooled to 40 ℃ of ， Jia ?enzyme 3.2g, stir insulation 2.5 hours.Filtration, filtrate is protein liquid, collects filtrate 3700ml, and concentration is 2%, separates and purifies, and concentrates, and drying gets protein hydrolysate powder 74g, and protein content 92% after testing.Filter residue adds 100ml water, adds the decolouring of 60g hydrogen peroxide again, washing, and after the centrifugal dehydration, the dry cellulose 105g that gets.
Claims (3)
1. one kind is that raw material extracts maltitol powder, protein hydrolysate powder and cellulosic method with wheat bran, it is characterized in that taking following steps:
A. the water that wheat bran is added 49 times of quality, be warming up to 90 ℃-100 ℃, be incubated 20-30 minute and carry out gelatinization, AMS and hemicellulase enzymolysis are added in gelatinization back well, wherein the AMS addition is the 0.0015%-0.008% of wheat bran raw material quality, the hemicellulase addition is the 0.0015%-0.009% of wheat bran raw material quality, and enzymolysis liquid filters, and filtrate is the Fructus Hordei Germinatus liquid glucose; Gained filtrate concentrates again through separation and purification, is drying to obtain maltitol powder;
B. the filter residue that last step enzymolysis liquid filtration obtains adds and is equivalent to wheat bran raw material quality 5-8 water doubly, add the compound protease that is equivalent to wheat bran raw material quality 0.008%, carried out enzymolysis process in 4-5 hour 40 ℃-75 ℃ insulations, enzymolysis liquid filters, and filtrate is hydrolyzed protein liquid; Filtrate is through purifying, concentrate, the dry protein hydrolysate powder that gets;
C. go up the step filter residue through decolouring, washing, centrifugal dehydration, the dry cellulose that gets.
2. according to claim 1 is that raw material extracts maltitol powder, protein hydrolysate powder and cellulosic method with wheat bran, it is characterized in that: a adds AMS earlier in the step, and regulatory enzyme enzymatic hydrolysis system pH value is to 5.5-7, keep 50 ℃-75 ℃ of hydrolysis temperatures, temperature retention time 60-80 minute; Cool to 40 ℃-55 ℃ then, with hemicellulase, regulatory enzyme enzymatic hydrolysis system pH value is incubated 3-4 hour to 4.5-6.5.
3. according to claim 1 is that raw material extracts maltitol powder, protein hydrolysate powder and cellulosic method with wheat bran, it is characterized in that: in the b step during compound protein enzymolysis, add the papain that is equivalent to wheat bran raw material quality 0.0015%-0.007% earlier, be warmed up to 40 ℃-75 ℃, regulatory enzyme enzymatic hydrolysis system pH value is incubated 2-3 hour to 4.5-7.5; Be incubated 40 ℃-75 ℃ then, sovolin in wheat bran raw material quality 0.0015%-0.007% De ?enzyme, be incubated 2-3 hour.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101607998B (en) * | 2009-05-14 | 2011-08-24 | 河南工业大学 | Method for transforming pentosan and dietary fiber by wheat bran enzyme engineering method |
| CN102174601A (en) * | 2011-01-05 | 2011-09-07 | 天津工业大学 | Method for producing raw material for fermentation, edible oil and protein by using highland barley bran |
| CN102308948A (en) * | 2011-07-14 | 2012-01-11 | 天津农学院 | Sauce food base stock produced by utilizing bran zymolyte and production method thereof |
| CN102839203A (en) * | 2012-09-19 | 2012-12-26 | 中粮生物化学（安徽）股份有限公司 | Enzymolysis method of starch raw material and method for preparing citric acid by fermenting |
| CN105010953A (en) * | 2015-06-02 | 2015-11-04 | 郑州轻工业学院 | Wheat bran dietary fiber and wheat bran small peptide cogeneration process |
| CN106174602A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | A kind of method preparing dietary fiber dry powder for raw material with Testa Tritici |
| CN106616185A (en) * | 2017-01-04 | 2017-05-10 | 云南农业大学 | Processing process of health type coix seed solid beverage |
| CN110063449A (en) * | 2019-04-29 | 2019-07-30 | 暨南大学 | A kind of processing method improving purple sweet potato face protein content |
| CN114592018A (en) * | 2020-12-03 | 2022-06-07 | 江苏省奥谷生物科技有限公司 | Production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues |
-
2008
- 2008-05-14 CN CNA2008100160486A patent/CN101278709A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101607998B (en) * | 2009-05-14 | 2011-08-24 | 河南工业大学 | Method for transforming pentosan and dietary fiber by wheat bran enzyme engineering method |
| CN102174601A (en) * | 2011-01-05 | 2011-09-07 | 天津工业大学 | Method for producing raw material for fermentation, edible oil and protein by using highland barley bran |
| CN102308948A (en) * | 2011-07-14 | 2012-01-11 | 天津农学院 | Sauce food base stock produced by utilizing bran zymolyte and production method thereof |
| CN102308948B (en) * | 2011-07-14 | 2012-10-03 | 天津农学院 | Sauce food base stock produced by utilizing bran zymolyte and production method thereof |
| CN102839203A (en) * | 2012-09-19 | 2012-12-26 | 中粮生物化学（安徽）股份有限公司 | Enzymolysis method of starch raw material and method for preparing citric acid by fermenting |
| CN102839203B (en) * | 2012-09-19 | 2014-01-01 | 中粮生物化学（安徽）股份有限公司 | Enzymolysis method of starch raw material and method for preparing citric acid by fermenting |
| CN105010953A (en) * | 2015-06-02 | 2015-11-04 | 郑州轻工业学院 | Wheat bran dietary fiber and wheat bran small peptide cogeneration process |
| CN106174602A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | A kind of method preparing dietary fiber dry powder for raw material with Testa Tritici |
| CN106616185A (en) * | 2017-01-04 | 2017-05-10 | 云南农业大学 | Processing process of health type coix seed solid beverage |
| CN110063449A (en) * | 2019-04-29 | 2019-07-30 | 暨南大学 | A kind of processing method improving purple sweet potato face protein content |
| CN114592018A (en) * | 2020-12-03 | 2022-06-07 | 江苏省奥谷生物科技有限公司 | Production method for preparing high maltose syrup and dietary fiber by using corn starch processing waste residues |
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