CN105433393A - Method for preparing dietary fiber from purple sweet potato residues used as raw materials - Google Patents
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Abstract
The invention discloses a method for preparing dietary fiber from purple sweet potato residues used as raw materials. The method comprises steps as follows: 1) the purple sweet potato residues after extraction of anthocyanin and starch are used as the raw materials, alpha-amylase is added for enzymolysis after the pH value is adjusted, then glucoamylase is added for saccharification, enzyme deactivation and filtration are performed, and a purple sweet potato saccharification liquid and purple sweet potato residues are obtained; 2) the purple sweet potato residues are desugared by ethanol and subjected to solid-liquid separation, and filter residues and a filtrate are obtained; 3) 0.8%-1.2% of a pepsase aqueous solution is added to the filter residues for hydrolysis, solid-liquid separation is performed, and filter residues and a filtrate are obtained; 4) the filter residues in Step 3) are taken, washed with water and dried, and insoluble dietary fiber is obtained; 5) the filtrate in Step 3) is taken, the pH (potential of hydrogen) of the filtrate is adjusted to range from 4.0 to 4.5, and centrifugation is performed; 6) a supernatant obtained in Step 5) is taken, concentrated and subjected to alcohol precipitation, and soluble dietary fiber is obtained. The method is simple, the insoluble dietary fiber and the soluble dietary fiber can be obtained simultaneously, and the content of the dietary fiber in obtained products is high.
Description
Technical field
The present invention relates to food processing technology field, being specifically related to a kind of take purple sweet potato as the method that dietary fiber prepared by raw material.
Background technology
Purple potato (Ipomoeabatatas (L.) Poir) has another name called black potato, and potato meat is that purple is to darkviolet.Purple potato not only has the nutritional labeling of common Ipomoea batatas, owing to being rich in anthocyanidin, dietary fiber, vitamin C, 8 kinds of vitamins such as B, A and 10 multi mineral prime element such as phosphorus, iron, also there is special health care, at anti-tired out, anticancer, anti-oxidant, enhancing immunity of organisms, remove interior free yl, prevent from playing important prevention effect in the generation of the diseases such as intestines and stomach.
The composition contained due to purple potato and health value thereof, be subject to common concern both domestic and external in recent years, and many enterprises add its processing ranks one after another, extracts the products such as anthocyanidin, purple sweet potato starch, the full powder of purple potato, purple potato drink, purple sweet potato liquor start to emerge as utilized purple potato.Wherein, the concern of people is received in purple potato containing abundant dietary fiber.
At present, from purple potato, extract anthocyanidin and starch is main product, in the production of production purple sweet potato anthocyanin, starch, also containing nutritional labelings such as a certain amount of anthocyanidin, starch, dietary fibers in its potato slag remaining after producing.But, falling as offal treatment the residue through extracting anthocyanidin and starch is normally direct in production, wasting raw material on the one hand, can contaminated environment be caused on the other hand.History radiance etc. discloses and also further for filter residue drying is obtained the dietary fiber (preparation of the product such as purple sweet potato anthocyanin optimization for extracting condition and starch while extraction purple sweet potato anthocyanin and starch, Food Science, 2014, Vol.35, No.22, p39-45), but simultaneously containing cellulose in filter residue powder, starch, protein and reduced sugar Multiple components, wherein content of cellulose (117.11 ± 2.69) mg/g, content of starch (761.03 ± 30.25) mg/g, protein content (50.48 ± 3.26) mg/g, content of reducing sugar (19.42 ± 0.56) mg/g, because in gained filter residue powder, dietary fiber content is not high, and content of starch is higher, thus purity is lower.
Publication number is that the patent of invention of CN104687067A discloses a kind of purple potato dietary fiber and preparation method thereof, the method comprises (1) adds water boil 15min ~ 20min by after purple potato stripping and slicing, be cooled to 70 DEG C ~ 75 DEG C, in cooling procedure, purple potato smashed to pieces in water, obtain purple potato solution; (2) by adding the amylase of the first scheduled volume in purple for step (1) gained potato solution, stirring and making starch liquefacation, being cooled to 40 DEG C ~ 45 DEG C; (3) in the purple potato solution after amylorrhexis, add the neutral proteinase of the second scheduled volume, stir and make proteolytic; (4) solution that step (3) obtains is carried out centrifugal solid-liquid separation; (5) step (4) gained solid is dried to water content less than 5%; (6), after being pulverized by dry gained solid, purple potato diet fiber product is obtained.This invention, has the following disadvantages for purple potato dietary fiber directly prepared by raw material with purple potato: one is that cost of material is higher; Two is with purple potato direct hydrolysis starch, because the amount of the high enzyme used of content of starch increases greatly, thus causes cost to increase; Adopt this technique also will remove deproteinize on the other hand, this had both added operation and had too increased cost.
Summary of the invention
It take purple sweet potato as the method that dietary fiber prepared by raw material that the technical problem to be solved in the present invention is to provide a kind of.The method is to prepare purple potato dietary fiber through extracting the purple sweet potato after anthocyanidin and starch for raw material, cost is low, in gained insoluble diedairy fiber product, insoluble diedairy fiber content is more than 73.1%, and wherein content of cellulose is 66.7%, and yield is more than 61.2%; In gained soluble dietary fiber product, soluble dietary fibre content is more than 85.6%, and wherein pectin content is more than 66.9%, and yield is more than 17.7%.
Described in this is the method that dietary fiber prepared by raw material with purple sweet potato, comprises the following steps:
1) with through extracting the purple sweet potato after anthocyanidin and starch for raw material, take citric acid solution as pH adjusting agent, the pH value of regulation system is 6.5 ~ 7.5, then adds AMS, is warming up to 70 DEG C ~ 80 DEG C enzymolysis 50min ~ 90min; Being cooled to 60 DEG C ~ 65 DEG C afterwards, is 4.0 ~ 4.5 by the pH value of citric acid solution regulation system, adds carbohydrase and carry out saccharification under heat-retaining condition, and go out after saccharification completely enzyme, filters, obtain purple potato saccharified liquid and purple potato residue respectively;
2) get step 1) the purple potato residue of gained add after dry, pulverize volumetric concentration be 80% ~ 90% ethanol carry out desugar, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
3) to step 2) add by the mass ratio of 1:15 ~ 25 the pepsin aqueous solution that mass concentration is 0.8% ~ 1.2% in gained filter residue, hydrolysis, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
4) step 3 is got) gained filter residue adds water washing, dry, obtains insoluble diedairy fiber (i.e. water insoluble dietary fiber);
5) step 3 is got) gained filtrate, regulate its pH value to be 4.0 ~ 4.5, leave standstill, centrifugal, collect supernatant;
6) step 5 is got) gained supernatant concentration, concentrate carries out alcohol precipitation, sediment separate out, dry, obtains soluble dietary fibre (i.e. water-soluble dietary fiber).
The step 1 of said method) in, the addition of AMS usually adds 6U ~ 10U AMS by every gram of purple sweet potato and calculates.The addition of carbohydrase usually adds 60U ~ 80U carbohydrase by every gram of purple sweet potato and calculates, and adopts absolute ethyl alcohol inspection saccharification mixture to represent that saccharification is complete to when generating (namely dextrin exists) without white precipitate; Be warming up to 70 DEG C ~ 90 DEG C enzymes that go out after saccharification completely, the time is generally 10min ~ 20min.By adding AMS and carbohydrase to remove starch remaining in purple sweet potato, meanwhile, the purple potato saccharified liquid of gained can also be made can be further used for making purple potato drink.
The step 2 of said method) in, desugar can be carried out under heating condition in heating or not, and under normal circumstances, desugar is carried out under 20 DEG C ~ 35 DEG C conditions, and the time of now desugar is 2h ~ 5h.The number of times of desugar can be defined as 1 time ~ 2 times as required.During desugar, volumetric concentration is 5 times ~ 15 times that the addition of the ethanol of 80% ~ 90% is generally dry rear purple potato residue weight.Usually aperture is adopted to be that the nylon filtering cloth of 50 μm ~ 80 μm filters after desugar.
The step 3 of said method) in, hydrolysis is carried out usually under 35 DEG C ~ 45 DEG C conditions, and the time of hydrolysis is generally 20min ~ 60min; Further selective hydrolysis is carried out under 40 DEG C of conditions.
The step 5 of said method) in, adopt citric acid solution to regulate the pH value of filtrate, specifically can regulate with the citric acid solution that pH value is 1 ~ 4.
The step 6 of said method) in, gained supernatant is normally concentrated into 1/3 ~ 1/5 of original volume, then carries out alcohol precipitation with the ethanol that volumetric concentration is 80% ~ 95%.
In the method for the invention, being used for the concentration of citric acid solution of adjust ph can determine as required, is generally the citric acid solution that pH value is 1 ~ 4.
Compared with prior art, the present invention is to prepare purple potato dietary fiber through extracting the purple sweet potato after anthocyanidin and starch for raw material, not only cost is low, and in gained insoluble diedairy fiber product insoluble diedairy fiber content more than 73.1%, wherein content of cellulose is more than 66.7%, and yield is more than 61.2%; In gained soluble dietary fiber product, soluble dietary fibre content is more than 85.6%, and pectin content is more than 66.9%, and yield is more than 17.7%.The method of the invention, while effectively utilizing discarded object, realizing resource regeneration, also avoid residue and directly abandons pollution on the environment.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and to understand content of the present invention better, but the present invention is not limited to following examples.
Embodiment 1
1) (namely to get purple potato to clean up through extracting the purple sweet potato after anthocyanidin and starch, the citric acid solution of 0.03w/w% is added by the weight ratio of 1:1, the rear separating obtained residue of broken, making beating) be raw material, get 10kg, with the pH value of the citric acid solution regulation system of pH=4 for 6.5, then add AMS, be warming up to 80 DEG C of enzymolysis 90min; Being cooled to 60 DEG C afterwards, is 4.5 by the pH value of the citric acid solution regulation system of pH=1, adds carbohydrase and carry out saccharification under heat-retaining condition, is warming up to 80 DEG C of enzyme 20min that go out after saccharification completely, filters, obtains purple potato saccharified liquid and purple potato residue respectively; Wherein, the addition of AMS adds 8U AMS by every gram of purple sweet potato and calculates, and the addition of carbohydrase adds 80U carbohydrase by every gram of purple sweet potato and calculates;
2) step 1 is got) the purple potato residue of gained dry, pulverize, and cross 40 mesh sieves, add the ethanol that volumetric concentration is 90% afterwards, desugar 2h under normal temperature condition by the slag liquor ratio of 1g:10g, gained mixture filters, and obtains filter residue and filtrate; Filter residue carries out desugar operation again by above-mentioned condition;
3) to step 2) add the pepsin aqueous solution that the mass concentration being equivalent to filter residue weight 20 times is 1.0%, 40 DEG C of Water Under solution 30min in gained filter residue, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
4) step 3 is got) gained filter residue adds the water agitator treating being equivalent to filter residue weight 10 times, filter, being dried to water content under 50 DEG C of conditions is 10.3%, obtains 6kg insoluble diedairy fiber product, wherein, insoluble diedairy fiber content is 73.7%, yield 60.7%, other compositions: protein content is 1.9%, fat content is 1.0%, content of starch is 10.9%, and anthocyanin content is 0.1%, and pectin content is 2.1%;
5) step 3 is got) gained filtrate, regulate its pH value to be 4.0 with the citric acid solution of pH=1, leave standstill, centrifugal (4000r/min, 10min), collect supernatant;
6) step 5 is got) gained supernatant concentration is to 1/5 of original volume, add in gained concentrate and be equivalent to concentrate volume 2 times, volumetric concentration be 80% ethanol precipitate, through standing, centrifugal (4000r/min, 10min), collecting precipitation thing, vacuum freezedrying is to water content < 10%, obtain 1.8kg soluble dietary fiber product, soluble dietary fibre content is 87.1%%, and wherein pectin content is 66.5%, and yield is 18.2%.
Embodiment 2
1) (namely to get purple potato to clean up through extracting the purple sweet potato after anthocyanidin and starch, stripping and slicing, the citric acid solution of 0.03w/w% is added by the weight ratio of 1:1, the rear separating obtained residue of broken, making beating) be raw material, get 10kg, with the pH value of the citric acid solution regulation system of pH=4 for 7.0, then add AMS, be warming up to 70 DEG C of enzymolysis 70min; Being cooled to 65 DEG C afterwards, is 4.0 by the pH value of the citric acid solution regulation system of pH=1, adds carbohydrase and carry out saccharification under heat-retaining condition, is warming up to 90 DEG C of enzyme 15min that go out after saccharification completely, filters, obtains purple potato saccharified liquid and purple potato residue respectively; Wherein, the addition of AMS adds 10U AMS by every gram of purple sweet potato and calculates, and the addition of carbohydrase adds 60U carbohydrase by every gram of purple sweet potato and calculates;
2) step 1 is got) the purple potato residue of gained dry, pulverize, and cross 60 mesh sieves, add the ethanol that volumetric concentration is 80% afterwards, desugar 5h under normal temperature condition by the slag liquor ratio of 1g:15g, gained mixture filters, and obtains filter residue and filtrate;
3) to step 2) add the pepsin aqueous solution that the mass concentration being equivalent to filter residue weight 25 times is 1.2%, 35 DEG C of Water Under solution 60min in gained filter residue, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
4) step 3 is got) gained filter residue adds the water agitator treating being equivalent to filter residue weight 8 times, filter, water content < 10% is dried under 60 DEG C of conditions, obtain 6kg insoluble diedairy fiber product, wherein, insoluble diedairy fiber content is 74.1%, and yield is 61.6%; Other compositions: protein content is 1.7%, fat content is 1.1%, and content of starch is 11.2%, and anthocyanin content is 0.1%, and pectin content is 2.2%;
5) step 3 is got) gained filtrate, regulate its pH value to be 4.2 with the citric acid solution of pH=1, leave standstill, centrifugal (3000r/min, 10min), collect supernatant;
6) step 5 is got) gained supernatant concentration is to 1/3 of original volume, add in gained concentrate and be equivalent to concentrate volume 1 times, volumetric concentration be 90% ethanol precipitate, through standing, centrifugal (4000r/min, 10min), collecting precipitation thing, vacuum freezedrying is to water content 10.1%, obtain 1.8kg soluble dietary fiber product, soluble dietary fibre content is 86.2%, and pectin content is 67.1%, and yield is 17.8%.
Embodiment 3
1) (namely to get purple potato to clean up through extracting the purple sweet potato after anthocyanidin and starch, stripping and slicing, the citric acid solution of 0.03w/w% is added by the weight ratio of 1:1, the rear separating obtained residue of broken, making beating) be raw material, get 10kg, with the pH value of the citric acid solution regulation system of pH=4.0 for 7.5, then add AMS, be warming up to 75 DEG C of enzymolysis 50min; Being cooled to 62 DEG C afterwards, is 4.2 by the pH value of the citric acid solution regulation system of pH=1, adds carbohydrase and carry out saccharification under heat-retaining condition, is warming up to 70 DEG C of enzyme 30min that go out after saccharification completely, filters, obtains purple potato saccharified liquid and purple potato residue respectively; Wherein, the addition of AMS adds 6U AMS by every gram of purple sweet potato and calculates, and the addition of carbohydrase adds 100U carbohydrase by every gram of purple sweet potato and calculates;
2) step 1 is got) the purple potato residue of gained dry, pulverize, and cross 40 mesh sieves, add the ethanol that volumetric concentration is 85% afterwards, desugar 3h under normal temperature condition by the slag liquor ratio of 1g:8g, gained mixture filters, and obtains filter residue and filtrate; Filter residue carries out desugar operation again by above-mentioned condition;
3) to step 2) add the pepsin aqueous solution that the mass concentration being equivalent to filter residue weight 15 times is 0.8%, 45 DEG C of Water Under solution 20min in gained filter residue, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
4) step 3 is got) gained filter residue adds the water agitator treating being equivalent to filter residue weight 10 times, filter, be dried to water content 9.9% under 55 DEG C of conditions, obtain 6kg insoluble diedairy fiber product, wherein, insoluble diedairy fiber content is 73.9%, yield 60.8%, other compositions: protein content is 1.8%, fat content is 1.3%, content of starch is 11.1%, and anthocyanin content is 0.1%, and pectin content is 2.0%;
5) step 3 is got) gained filtrate, regulate its pH value to be 4.5 with the citric acid solution of pH=1, leave standstill, centrifugal (4000r/min, 10min), collect supernatant;
6) step 5 is got) gained supernatant concentration is to 1/4 of original volume, add in gained concentrate and be equivalent to concentrate volume 2 times, volumetric concentration be 90% ethanol precipitate, through standing, centrifugal (4000r/min, 10min), collecting precipitation thing, vacuum freezedrying is to water content < 10%, obtain 1.8kg soluble dietary fiber product, soluble dietary fibre content is 85.9%, and wherein pectin content is 66.8%, and yield is 18.3%.
Claims (6)
1. be the method that dietary fiber prepared by raw material with purple sweet potato, comprise the following steps:
1) with through extracting the purple sweet potato after anthocyanidin and starch for raw material, take citric acid solution as pH adjusting agent, the pH value of regulation system is 6.5 ~ 7.5, then adds AMS, is warming up to 70 DEG C ~ 80 DEG C enzymolysis 50min ~ 90min; Being cooled to 60 DEG C ~ 65 DEG C afterwards, is 4.0 ~ 4.5 by the pH value of citric acid solution regulation system, adds carbohydrase and carry out saccharification under heat-retaining condition, and go out after saccharification completely enzyme, filters, obtain purple potato saccharified liquid and purple potato residue respectively;
2) get step 1) the purple potato residue of gained add after dry, pulverize volumetric concentration be 80% ~ 90% ethanol carry out desugar, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
3) to step 2) add by the mass ratio of 1:15 ~ 25 the pepsin aqueous solution that mass concentration is 0.8% ~ 1.2% in gained filter residue, hydrolysis, gained mixture carries out Separation of Solid and Liquid, obtains filter residue and filtrate;
4) step 3 is got) gained filter residue adds water washing, dry, obtains insoluble diedairy fiber;
5) step 3 is got) gained filtrate, regulate its pH value to be 4.0 ~ 4.5, leave standstill, centrifugal, collect supernatant;
6) step 5 is got) gained supernatant concentration, concentrate carries out alcohol precipitation, sediment separate out, dry, obtains soluble dietary fiber.
2. method according to claim 1, is characterized in that: step 1) in, the addition of AMS adds 6U ~ 10U AMS by every gram of purple sweet potato and calculates.
3. method according to claim 1, is characterized in that: step 1) in, the addition of carbohydrase adds 60U ~ 80U carbohydrase by every gram of purple sweet potato and calculates.
4. method according to claim 1, is characterized in that: step 2) in, desugar is in heating or do not carry out under heating condition.
5. method according to claim 1, is characterized in that: step 2) in, desugar is carried out under 20 DEG C ~ 35 DEG C conditions, and the time of desugar is 2h ~ 5h.
6. method according to claim 1, is characterized in that: step 3) in, be hydrolyzed and carry out under 35 DEG C ~ 45 DEG C conditions, the time of hydrolysis is 20min ~ 60min.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106262954A (en) * | 2016-08-24 | 2017-01-04 | 徐州工程学院 | A kind of purple sweet potato dietary fiber sheet and preparation method thereof |
| CN106579423A (en) * | 2016-11-24 | 2017-04-26 | 青岛大学 | Comprehensive extraction method for various effective components in purple sweet potatoes |
| CN107712917A (en) * | 2017-09-18 | 2018-02-23 | 炉霍雪域俄色有限责任公司 | A kind of method for extracting Malus toringoides Hughes dietary fiber |
| CN108713763A (en) * | 2018-03-26 | 2018-10-30 | 内江师范学院 | A kind of high-quality lemon diet fibers complex enzyme preparation method |
| CN110025015A (en) * | 2019-04-15 | 2019-07-19 | 江苏师范大学 | The extracting method of sweet potato dregs diet fibre and its application in adjusting intestinal flora |
| CN110150679A (en) * | 2019-06-06 | 2019-08-23 | 恒源生物科技有限公司 | A kind of method of multienzyme method of fractional steps preparation pea slag insoluble dietary fiber |
| CN111493317A (en) * | 2020-04-17 | 2020-08-07 | 广州大学 | Method for extracting dietary fiber-bonded phenol adduct in fruit pulp |
| CN112159270A (en) * | 2020-09-24 | 2021-01-01 | 广西师范大学 | Citrus planting fertilizer and preparation method thereof |
| CN114098083A (en) * | 2021-12-02 | 2022-03-01 | 武汉轻工大学 | Purple sweet potato ingredient and preparation method and application thereof |
| CN114146183A (en) * | 2021-12-02 | 2022-03-08 | 武汉轻工大学 | Purple sweet potato blood sugar-reducing polypeptide microencapsulated product and preparation method and application thereof |
| CN115606813A (en) * | 2022-10-13 | 2023-01-17 | 中国林业科学研究院林产化学工业研究所 | Myrtle dietary fiber with anti-saccharification activity and preparation method thereof |
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| CN106262954A (en) * | 2016-08-24 | 2017-01-04 | 徐州工程学院 | A kind of purple sweet potato dietary fiber sheet and preparation method thereof |
| CN106579423A (en) * | 2016-11-24 | 2017-04-26 | 青岛大学 | Comprehensive extraction method for various effective components in purple sweet potatoes |
| CN107712917A (en) * | 2017-09-18 | 2018-02-23 | 炉霍雪域俄色有限责任公司 | A kind of method for extracting Malus toringoides Hughes dietary fiber |
| CN108713763A (en) * | 2018-03-26 | 2018-10-30 | 内江师范学院 | A kind of high-quality lemon diet fibers complex enzyme preparation method |
| CN110025015A (en) * | 2019-04-15 | 2019-07-19 | 江苏师范大学 | The extracting method of sweet potato dregs diet fibre and its application in adjusting intestinal flora |
| CN110150679A (en) * | 2019-06-06 | 2019-08-23 | 恒源生物科技有限公司 | A kind of method of multienzyme method of fractional steps preparation pea slag insoluble dietary fiber |
| CN111493317A (en) * | 2020-04-17 | 2020-08-07 | 广州大学 | Method for extracting dietary fiber-bonded phenol adduct in fruit pulp |
| CN112159270A (en) * | 2020-09-24 | 2021-01-01 | 广西师范大学 | Citrus planting fertilizer and preparation method thereof |
| CN114098083A (en) * | 2021-12-02 | 2022-03-01 | 武汉轻工大学 | Purple sweet potato ingredient and preparation method and application thereof |
| CN114146183A (en) * | 2021-12-02 | 2022-03-08 | 武汉轻工大学 | Purple sweet potato blood sugar-reducing polypeptide microencapsulated product and preparation method and application thereof |
| CN115606813A (en) * | 2022-10-13 | 2023-01-17 | 中国林业科学研究院林产化学工业研究所 | Myrtle dietary fiber with anti-saccharification activity and preparation method thereof |
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