CN104829734A - Method of producing pigment, protein, polysaccharide and dietary fiber from dry lentinula edodes - Google Patents
Method of producing pigment, protein, polysaccharide and dietary fiber from dry lentinula edodes Download PDFInfo
- Publication number
- CN104829734A CN104829734A CN201410535782.9A CN201410535782A CN104829734A CN 104829734 A CN104829734 A CN 104829734A CN 201410535782 A CN201410535782 A CN 201410535782A CN 104829734 A CN104829734 A CN 104829734A
- Authority
- CN
- China
- Prior art keywords
- mushroom
- pigment
- residue
- dried thin
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000049 pigment Substances 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 26
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 25
- 240000000599 Lentinula edodes Species 0.000 title claims abstract description 24
- 235000001715 Lentinula edodes Nutrition 0.000 title claims abstract description 24
- 150000004676 glycans Chemical class 0.000 title claims abstract description 24
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 24
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 24
- 239000000284 extract Substances 0.000 claims abstract description 23
- 235000013305 food Nutrition 0.000 claims abstract description 18
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 100
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 44
- 239000007788 liquid Substances 0.000 claims description 43
- 238000000605 extraction Methods 0.000 claims description 37
- 238000000108 ultra-filtration Methods 0.000 claims description 25
- 229920001491 Lentinan Polymers 0.000 claims description 23
- 229940115286 lentinan Drugs 0.000 claims description 23
- 239000012153 distilled water Substances 0.000 claims description 22
- 239000012528 membrane Substances 0.000 claims description 22
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 238000005119 centrifugation Methods 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 17
- 239000006228 supernatant Substances 0.000 claims description 16
- 239000000835 fiber Substances 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- -1 albumen Substances 0.000 claims description 10
- 239000003205 fragrance Substances 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 230000004071 biological effect Effects 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 235000008504 concentrate Nutrition 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000010410 dusting Methods 0.000 claims description 2
- 235000014666 liquid concentrate Nutrition 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000005374 membrane filtration Methods 0.000 abstract description 3
- 238000000053 physical method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 238000012360 testing method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 7
- 230000036541 health Effects 0.000 description 6
- 230000035764 nutrition Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000011161 development Methods 0.000 description 5
- 239000002585 base Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 238000007873 sieving Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 229920002774 Maltodextrin Polymers 0.000 description 2
- 239000005913 Maltodextrin Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 229940035034 maltodextrin Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000222501 Agaricaceae Species 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 101100394237 Mus musculus Hand1 gene Proteins 0.000 description 1
- 208000030768 Optic nerve injury Diseases 0.000 description 1
- YUGCAAVRZWBXEQ-UHFFFAOYSA-N Precholecalciferol Natural products C=1CCC2(C)C(C(C)CCCC(C)C)CCC2C=1C=CC1=C(C)CCC(O)C1 YUGCAAVRZWBXEQ-UHFFFAOYSA-N 0.000 description 1
- 208000019155 Radiation injury Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000078 anti-malarial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003430 antimalarial agent Substances 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000003393 splenic effect Effects 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a comprehensive utilization technology of dry lentinula edodes and includes the method of preparing lentinula edodes pigment, lentinula edodes protein, lentinula edodes polysaccharide and lentinula edodes dietary fiber. The method is advantaged in that by means of physical methods such as low temperature, high temperature, membrane filtration and the like, low-cost comprehensive utilization of the lentinula edodes is achieved, and the bioactivity the extracts can be maximumly maintained and meanwhile the safety of the extracts is ensured. The pigment, the protein, the polysaccharide and the dietary fiber can be added to foods directly and are not only nutritional and health-caring but also safe.
Description
Technical field
The present invention relates to a kind of deep-processing and colligating-using technique of dried thin mushroom, particularly relate to a kind of method simultaneously extracting mushroom pigment, Lentinus Edodes protein, lentinan and mushroom dietary fiber from dried thin mushroom.
Background technology
Mushroom is the sporophore of Mycophyta door fungi mushroom, belongs to Basidiomycetes Agaricaceae, is that edible mushrooms famous is in the world held concurrently one of medicinal fungus.Due to nutritious, fragrance oozes spleen, and delicious flavour, have the laudatory title of " king in mushroom ", " mushroom queen ", " hats of vegetables ".
Mushroom has high protein, lower fat, multiple amino acids and multivitamin mushroom food.The edible part of dried thin mushroom accounts for 72%, and every 100g eats moisture 13g in part, fatty 1.8g, protein 17g, carbohydrate 54g, robust fibre 7.8g, ash content 4.9g.
Scientific research a large amount of both at home and abroad at present has demonstrated the multiple biological activity of lentinan, and such as lentinan can excite the foundation of mouse Th1 type immunne response; Can recover and protect the splenic function of rat bearing S180, enhancing body immunologic function; Antitumor, oxidation resistant effect is had to rat; Influenza virus can be suppressed to copy in vivo; Can anti-listeria infect, anti-malarial; Provide protection is had to radiation injury; Can alleviating fatigue; Also provide protection is had to optic nerve injury.Correlative study also shows: lentinan has activating cells immunity, regulates multiple humoral indications, induces alpha-interferon to generate, regulate body immune response, induction white corpuscle, to tumor-infiltrated, causes tumor locus vasodilation, hemorrhage, downright bad, stops the combination of virus and host cell, improve SOD active, suppress MDA to generate, lipid peroxidation inhibition, reduce cholesterol, regulate carbohydrate metabolism, improve sugar tolerance, expand gi tract generation satiety and alleviate appetite, reduce the effects such as blood sugar.
In the prior art, some are all had to report at home and abroad to the Extraction and isolation of Lentinan and albumen, but the preparation of lentinan all needs to adopt ethanol to be settled from dilute solution by polysaccharide in document, the preparation of Lentinus Edodes protein then needs to adopt alkali to put forward the method for alcohol precipitation, all need in these techniques to use ethanol, on the one hand because ethanol consumption is large, cost is high; On the other hand because ethanol is flammable, there is potential safety hazard, the industrialization constraining lentinan is to a certain extent produced.
If the production technique of a kind of safety, low cost can be studied, while extraction lentinan, by other nutritive ingredients in mushroom also extraction and application in the lump, that will promote the lifting of mushroom process deeply industry state of the art greatly, realizes the conversion increment of mushroom.
Summary of the invention
The object of the present invention is to provide a kind of comprehensive utilization process of dried thin mushroom, can extract in the process simultaneously and obtain mushroom pigment, Lentinus Edodes protein, lentinan and mushroom dietary fiber, realize making full use of resource to greatest extent, low and the safety of process energy consumption, meet the resource-conserving Development Strategy required by current China, also meet simultaneously and extend agricultural-food industrial chain, drive the development thought that agricultural-food industrialization level and agricultural-food comprehensive benefit improve comprehensively.
Technical scheme of the present invention comprises the following steps:
(1) dried thin mushroom is pulverized: pulverized and sieved by dried thin mushroom, require that the grinding particle size of material is between 20 ~ 60 orders;
(2) cold water lixiviate: by the material after pulverizing by solid-liquid mass ratio 1:(20 ~ 25) add water and prepare burden, after stirring, at 0 ~ 5 DEG C, soak 35 ~ 45min;
(3) scalping: above-mentioned cold mixture of carrying is crossed 80 ~ 120 mesh sieves, screen overflow is used for step (6), enters next step (4) by the cold extract of sieve;
(4) dusting cover: the cold extract that step (3) obtains is crossed 300 ~ 400 mesh sieves, and screen overflow discards, enters next step (5) by the filtrate of sieve;
(5) ultrafiltration: by filtrate 0.4 ~ 0.6MPa, 20 ~ 40 DEG C, be the ultra-filtration membrane of 5000 ~ 10000 by molecular weight cut-off, be not 1:8 ~ 1:10 through the dope volume of film and the ratio of stoste volume, obtain mushroom pigment through after the clear liquid concentrate drying of ultra-filtration membrane, after not adopting vacuum and low temperature liquid continuous drier to carry out drying through the dope of ultra-filtration membrane, obtain Lentinus Edodes protein;
(6) hot water extraction: by the screen overflow in step (3) by solid-liquid mass ratio 1:(4 ~ 5) adding distil water prepares burden, after stirring, add the citric acid of dried thin mushroom weight 1.8% ~ 2.2%, 55 ~ 65min is extracted at 85 ~ 95 DEG C, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for second extraction, the distilled water of residue 4 ~ 5 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out second time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for three times and extracts, the distilled water of residue 3 ~ 4 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out third time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue enters next step drying process, merge the supernatant liquor of above-mentioned three times and cross 300 ~ 400 mesh sieves, lentinan is obtained by the filtrate concentrate drying of sieve,
(7) residue is dry: by above-mentioned centrifugal after residue dry under 70 ~ 90 DEG C of conditions, pulverized 60 ~ 80 mesh sieves, can mushroom dietary fiber be obtained.
Just because of well-designed to above technique of contriver, each step is closely connected, all linked with one another, just achieves the comprehensive development and utilization of dried thin mushroom safety, low cost.Advantages found of the present invention formerly adopts low temperature immersing extraction to go out the material such as soluble proteins and small molecules carbohydrate, pigment in dried thin mushroom, make foreign matter content in the lentinan of subsequent extracted few, of light color, not needing to add maltodextrin directly can carry out spraying dry; In addition, this method can extract the lentinan of in dried thin mushroom more than 90%, and this effective constituent is fully utilized.Advantage of the present invention is also embodied in the separation of Lentinus Edodes protein and dry employing ultra-filtration and separation is concentrated and low temperature continous vacuum belt drying technology, Lentinus Edodes protein is separated with small-molecule substance, both achieved under normal temperature be separated, concentrated, dry Lentinus Edodes protein, the decomposition avoiding activeconstituents destroys, and synchronously can realize again decolouring and the preliminary purification of lentinan.
Specific implementation process of the present invention comprises the following steps:
(1) dried thin mushroom is pulverized and sieved, require that the grinding particle size of material is between 20 ~ 60 orders;
(2) material after pulverizing is by solid-liquid mass ratio 1:(20 ~ 25) add water and prepare burden, after stirring, at 0 ~ 5 DEG C, soak 35 ~ 45min;
(3) above-mentioned cold mixture of carrying is crossed 80 ~ 120 mesh sieves, the water content standard of screen overflow is: when firmly holding with hand, anhydrously oozes out;
(4) the cold extract having crossed 80 ~ 120 mesh sieves was continued 300 ~ 400 mesh sieves, screen overflow discards;
(5) by the extracting solution of having crossed 300 ~ 400 mesh sieves 0.4 ~ 0.6MPa, 20 ~ 40 DEG C, be the ultra-filtration membrane of 5000 ~ 10000 by molecular weight cut-off, be not 1:8 ~ 1:10 through the dope volume of film and the ratio of stoste volume, clear liquid through ultra-filtration membrane need carry out concentrating under reduced pressure at 75 ~ 85 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:10 ~ 1:12, then 80 ~ 90 DEG C of oven dry, the mushroom pigment obtained is very easily water-soluble, and with strong mushroom fragrance, can be used as natural essence and pigment; Dope not through ultra-filtration membrane adopts vacuum and low temperature liquid continuous drier to carry out drying, and temperature controls between 25 ~ 35 DEG C, and vacuum tightness is ﹣ 0.095 ~ ﹣ 0.099MPa, thus guarantees the biological activity of Lentinus Edodes protein;
(6) by the screen overflow of 80 ~ 120 mesh sieves by solid-liquid mass ratio 1:(4 ~ 5) adding distil water prepares burden, solid-to-liquid ratio is now the weight in wet base of screen overflow and the ratio of distilled water, as with the weighing scale of dried thin mushroom, to prepare burden in ratio below: the cold weight carrying rear wet feed is about 3 ~ 4 times of siccative weight, add the distilled water of 4 ~ 5 times on this basis again, after stirring, add the citric acid of dried thin mushroom weight 1.8% ~ 2.2%, 55 ~ 65min is extracted at 85 ~ 95 DEG C, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for second extraction, the distilled water of residue 4 ~ 5 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out second time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for three times and extracts, the distilled water of residue 3 ~ 4 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out third time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue enters next step drying process, merge the supernatant liquor of above-mentioned three times and cross 300 ~ 400 mesh sieves, concentrating under reduced pressure is carried out at 80 ~ 90 DEG C by the filtrate of sieve, the volume of concentrated solution and the ratio of stoste volume are 1:8 ~ 1:10, then it is 180 ~ 190 DEG C in inlet temperature, air outlet temperature is carry out spraying dry while hot under the condition of 90 ~ 100 DEG C, obtain lentinan powder,
(7) by above-mentioned centrifugal after residue dry under 70 ~ 90 DEG C of conditions, pulverized 60 ~ 80 mesh sieves, can mushroom dietary fiber be obtained.
When aforesaid method carries out, in order to ensure the permeability of ultra-filtration membrane, as the case may be ultra-filtration membrane is cleaned at use 2 ~ 3 all after dates.During cleaning, first taken out of by the sample liquid remained in film with pure water, then wash by the NaOH solution of pH=11 ~ 12, pressure-controlling is at 0.2 ~ 0.4MPa, and temperature controls at 20 ~ 40 DEG C, and when effluent liquid is close to time colourless, alkali cleaning terminates, then uses pure water rinsing.
In sum, advantage of the present invention is can extract from dried thin mushroom simultaneously and obtains the product that mushroom pigment, Lentinus Edodes protein, lentinan and mushroom dietary fiber four kinds has nutrition and health care function concurrently.First, the mushroom pigment that the present invention obtains not only remains the reducing sugar of 1.5% and the oligose of 2% in dried thin mushroom, also has strong mushroom fragrance, can as the good source of natural pigment, not only nutritive health-care but also safety; Secondly, the Lentinus Edodes protein that the present invention obtains adopts low temperature extraction process by water, membrane filtration and low temperature continous vacuum belt drying technology to produce, with existing use alkalinity extraction or adopt different solvents to carry out compared with grading extraction, extraction time obviously shortens, extraction efficiency significantly improves, biological activity obviously strengthens, and the Lentinus Edodes protein powder adopting the present invention to obtain has good water-soluble, can widespread use in food; Again, the lentinan that the present invention obtains adopts step by step arithmetic in short-term, leaching process does not use ethanol, only with the addition of the citric acid of food grade, spray-drying process does not need to add maltodextrin yet, compared with existing method, and the polysaccharide lighter color of acquisition, extraction time is short, and extraction is complete and security is high; Finally, the mushroom dietary fiber that the present invention obtains remains fibers a large amount of in mushroom, ash content, mineral substance and provitamin D, has good nourishing function, can extensively make an addition in food.This invention not only makes various nutrition and health care composition in mushroom obtain fully effective utilization, and the whole explained hereafter cycle is short, and extraction yield is high, cost-saving and energy consumption, easy and simple to handle, achieves the comprehensive development and utilization of mushroom low cost.
Embodiment
Following examples are intended to the present invention instead of limitation of the invention further are described.
Embodiment 1
Dried thin mushroom (perigone mushroom) is crushed to 20 orders, adds water by solid-liquid mass ratio 1:20 and prepare burden, after stirring, at 0 DEG C, soak 40min; Cold mixture of carrying crosses 100 mesh sieves, and the water content standard of stand-by screen overflow is: when firmly holding with hand, anhydrously oozes out; Filtered solution continued 300 mesh sieves, and screen overflow discards; Filtered solution 0.5MPa, 40 DEG C, be the ultra-filtration membrane of 5000 by molecular weight cut-off, be not 1:8 through the dope volume of film and the ratio of stoste volume, clear liquid through ultra-filtration membrane carries out concentrating under reduced pressure at 80 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:10, then 85 DEG C of oven dry, mushroom pigment can be obtained.This pigment is very easily water-soluble, and with strong mushroom fragrance, the aqueous solution is shiny red, and this pigment remains the reducing sugar of 1.43% in mushroom dry powder, the oligose of 2.08% after measured, can be used as natural essence and pigment; The vacuum and low temperature liquid continuous drier not adopting Shanghai Minjie Machinery Co. Ltd. to develop through the dope of ultra-filtration membrane carries out drying, temperature of charge is 30 DEG C, vacuum tightness is ﹣ 0.098MPa, the Lentinus Edodes protein powder obtained has good instantly-soluble, yield is 8.6%, after testing, containing 34.2% protein, the soluble dietary fibre of 3.1%, the insoluble dietary fibre of 1.8%.
Prepared burden by solid-liquid mass ratio 1:4.5 adding distil water by the stand-by screen overflow of 100 mesh sieves, solid-to-liquid ratio is now the weight in wet base of screen overflow and the ratio of distilled water, as with the weighing scale of dried thin mushroom, to prepare burden in ratio below: the cold weight carrying rear wet feed is 3.3 times of siccative weight, adds the distilled water of 4.5 times on this basis again, after stirring, add the citric acid of dried thin mushroom weight 1.9%, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 4000r/min, centrifugation time 5min, collect clear liquid, residue is used for second extraction, the distilled water of residue 4 times of volumes is added in residue, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 4000r/min, centrifugation time 5min, collect clear liquid, residue is used for three times and extracts, the distilled water of residue 3 times of volumes is added in residue, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 4000r/min, centrifugation time 5min, collect clear liquid, merge the supernatant liquor of three times and cross 300 mesh sieves, clear liquid after sieving is carried out concentrating under reduced pressure at 85 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:8, then it is 180 DEG C in inlet temperature, air outlet temperature is carry out spraying dry while hot under the condition of 90 DEG C, obtain flaxen lentinan powder, yield is 13.3%, after testing, polysaccharide content is 43%, insoluble dietary fibre content is 0.28%.Dried under 70 DEG C of conditions by residue after centrifugal for above-mentioned third time, pulverized 60 mesh sieves, can obtain mushroom dietary fiber, after testing, its polysaccharide content is 0.65%, shows that the soluble saccharide of in dried thin mushroom more than 90% is effectively separated.By this technique, the activeconstituents in mushroom obtains enrichment in various degree, forms the multiple product with nutrition and health care function, achieves mushroom comprehensive utilization and maximizes.
Embodiment 2
Dried thin mushroom (937) is crushed to 40 orders, adds water by solid-liquid mass ratio 1:25 and prepare burden, after stirring, at 4 DEG C, soak 35min; Cold mixture of carrying crosses 80 mesh sieves, and the water content standard of stand-by screen overflow is: when firmly holding with hand, anhydrously oozes out; Filtered solution continued 300 mesh sieves, and screen overflow discards; Filtered solution 0.5MPa, 35 DEG C, be the ultra-filtration membrane of 5000 by molecular weight cut-off, be not 1:9 through the dope volume of film and the ratio of stoste volume, clear liquid 85 DEG C through ultra-filtration membrane carries out concentrating under reduced pressure, the volume of concentrated solution and the ratio of stoste volume are 1:11, then 90 DEG C of oven dry, mushroom pigment can be obtained.This pigment is very easily water-soluble, and with strong mushroom fragrance, the aqueous solution is shiny red, and this pigment remains the reducing sugar of 1.49% in mushroom dry powder, the oligose of 2.18% after measured, can be used as natural essence and pigment; The vacuum and low temperature liquid continuous drier not adopting Shanghai Minjie Machinery Co. Ltd. to develop through the dope of ultra-filtration membrane carries out drying, temperature of charge is 30 DEG C, vacuum tightness is ﹣ 0.098MPa, the Lentinus Edodes protein powder obtained has good instantly-soluble, yield is 8.9%, after testing, containing 35.8% protein, the soluble dietary fibre of 3.4%, the insoluble dietary fibre of 1.9%.
Prepared burden by solid-liquid mass ratio 1:5 adding distil water by the stand-by screen overflow of 80 mesh sieves, solid-to-liquid ratio is now the weight in wet base of screen overflow and the ratio of distilled water, as with the weighing scale of dried thin mushroom, to prepare burden in ratio below: the cold weight carrying rear wet feed is 3.8 times of siccative weight, adds the distilled water of 5 times on this basis again, after stirring, add the citric acid of dried thin mushroom weight 2%, 60min is extracted at 95 DEG C, after extraction terminates, centrifugal, revolution is 4500r/min, centrifugation time 8min, collect clear liquid, residue is used for second extraction, the distilled water of residue 4.5 times of volumes is added in residue, 60min is extracted at 95 DEG C, after extraction terminates, centrifugal, revolution is 4500r/min, centrifugation time 8min, collect clear liquid, residue is used for three times and extracts, the distilled water of residue 3.5 times of volumes is added in residue, 60min is extracted at 95 DEG C, after extraction terminates, centrifugal, revolution is 4500r/min, centrifugation time 8min, collect clear liquid, merge the supernatant liquor of three times and cross 300 mesh sieves, clear liquid after sieving is carried out concentrating under reduced pressure at 80 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:9, then it is 190 DEG C in inlet temperature, air outlet temperature is carry out spraying dry while hot under the condition of 95 DEG C, obtain lentinan powder, yield is 13.8%, after testing, polysaccharide content is 45%, insoluble dietary fibre content is 0.33%.Dried under 80 DEG C of conditions by residue after centrifugal for above-mentioned third time, pulverized 80 mesh sieves, can obtain mushroom dietary fiber, after testing, its polysaccharide content is 0.61%, shows that the soluble saccharide of in dried thin mushroom more than 90% is effectively separated.By this technique, the activeconstituents in mushroom obtains enrichment in various degree, forms the multiple product with nutrition and health care function, achieves mushroom comprehensive utilization and maximizes.
Embodiment 3
Dried thin mushroom (937) is crushed to 60 orders, adds water by solid-liquid mass ratio 1:25 and prepare burden, after stirring, at 4 DEG C, soak 45min; Cold mixture of carrying crosses 120 mesh sieves, and the water content standard of stand-by screen overflow is: when firmly holding with hand, anhydrously oozes out; Filtered solution continued 300 mesh sieves, and screen overflow discards; Filtered solution 0.6MPa, 35 DEG C, be the ultra-filtration membrane of 10000 by molecular weight cut-off, be not 1:10 through the dope volume of film and the ratio of stoste volume, clear liquid 80 DEG C through ultra-filtration membrane carries out concentrating under reduced pressure, the volume of concentrated solution and the ratio of stoste volume are 1:12, then 80 DEG C of oven dry, mushroom pigment can be obtained.This pigment is very easily water-soluble, and with strong mushroom fragrance, the aqueous solution is shiny red, and this pigment remains the reducing sugar of 1.53% in mushroom dry powder, the oligose of 2.24% after measured, can be used as natural essence and pigment; The vacuum and low temperature liquid continuous drier not adopting Shanghai Minjie Machinery Co. Ltd. to develop through the dope of ultra-filtration membrane carries out drying, temperature of charge is 30 DEG C, vacuum tightness is ﹣ 0.098MPa, the Lentinus Edodes protein powder obtained has good instantly-soluble, yield is 9.1%, after testing, containing 36.7% protein, the soluble dietary fibre of 3.1%, the insoluble dietary fibre of 1.7%.
Prepared burden by solid-liquid mass ratio 1:4 adding distil water by the stand-by screen overflow of 120 mesh sieves, solid-to-liquid ratio is now the weight in wet base of screen overflow and the ratio of distilled water, as with the weighing scale of dried thin mushroom, to prepare burden in ratio below: the cold weight carrying rear wet feed is 4 times of siccative weight, adds the distilled water of 4 times on this basis again, after stirring, add the citric acid of dried thin mushroom weight 2.2%, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 5000r/min, centrifugation time 10min, collect clear liquid, residue is used for second extraction, the distilled water of residue 4 times of volumes is added in residue, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 5000r/min, centrifugation time 10min, collect clear liquid, residue is used for three times and extracts, the distilled water of residue 4 times of volumes is added in residue, 60min is extracted at 90 DEG C, after extraction terminates, centrifugal, revolution is 5000r/min, centrifugation time 10min, collect clear liquid, merge the supernatant liquor of three times and cross 300 mesh sieves, clear liquid after sieving is carried out concentrating under reduced pressure at 90 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:10, then it is 180 DEG C in inlet temperature, air outlet temperature is carry out spraying dry while hot under the condition of 100 DEG C, obtain lentinan powder, yield is 14.1%, after testing, polysaccharide content is 46%, insoluble dietary fibre content is 0.37%.Dried under 90 DEG C of conditions by residue after centrifugal for above-mentioned third time, pulverized 80 mesh sieves, can obtain mushroom dietary fiber, after testing, its polysaccharide content is 0.66%, shows that the soluble saccharide of in dried thin mushroom more than 90% is effectively separated.By this technique, the activeconstituents in mushroom obtains enrichment in various degree, forms the multiple product with nutrition and health care function, achieves mushroom comprehensive utilization and maximizes.
The present invention is by the use of the physical methods such as low temperature, high temperature, membrane filtration, achieve the comprehensive utilization of mushroom low cost, maintain the biological activity of each extract to greatest extent, in turn ensure that its security, the pigment produced by this method, albumen, polysaccharide, food fibre can directly add in food, not only nutritive health-care but also safety.Secondly the mushroom pigment produced by this method is very easily water-soluble and have strong mushroom fragrance, wherein containing the reducing sugar of 1.5%, the oligose of 2% in dried thin mushroom, can be used as natural essence and pigment; The Lentinus Edodes protein produced by this method has good instantly-soluble, and yield is about 9%, and wherein protein content is 35%, and soluble dietary fibre content is 3.3%, and insoluble dietary fibre content is 1.8%; The lentinan produced by this method is pale yellow powder, and yield is about 14%, and its polysaccharide content is 45%, and the content of insoluble dietary fibre is 0.3%; Residue after extraction can be used as the good source of food fibre, wherein the content of soluble polysaccharide is only 0.6%, by this technique, the polysaccharide of in dried thin mushroom more than 90% can be extracted, and form the multiple product having nutrition and health care function concurrently, this not only can reduce the production cost of mushroom deep processing enterprise, improves value-added content of product, and can promote the development of mushroom plant husbandry and food service industry.
Claims (8)
1. utilize dried thin mushroom to produce a method for pigment, albumen, polysaccharide and food fibre, it is characterized in that, comprise the following steps:
(1) dried thin mushroom is pulverized: pulverized and sieved by dried thin mushroom, require that the grinding particle size of material is between 20 ~ 60 orders;
(2) cold water lixiviate: by the material after pulverizing by solid-liquid mass ratio 1:(20 ~ 25) add water and prepare burden, after stirring, at 0 ~ 5 DEG C, soak 35 ~ 45min;
(3) scalping: above-mentioned cold mixture of carrying is crossed 80 ~ 120 mesh sieves, screen overflow is used for step (6), enters next step (4) by the cold extract of sieve;
(4) dusting cover: the cold extract that step (3) obtains is crossed 300 ~ 400 mesh sieves, and screen overflow discards, enters next step (5) by the filtrate of sieve;
(5) ultrafiltration: by filtrate 0.4 ~ 0.6MPa, 20 ~ 40 DEG C, be the ultra-filtration membrane of 5000 ~ 10000 by molecular weight cut-off, be not 1:8 ~ 1:10 through the dope volume of film and the ratio of stoste volume, obtain mushroom pigment through after the clear liquid concentrate drying of ultra-filtration membrane, after not adopting vacuum and low temperature liquid continuous drier to carry out drying through the dope of ultra-filtration membrane, obtain Lentinus Edodes protein;
(6) hot water extraction: by the screen overflow in step (3) by solid-liquid mass ratio 1:(4 ~ 5) adding distil water prepares burden, after stirring, add the citric acid of dried thin mushroom weight 1.8% ~ 2.2%, 55 ~ 65min is extracted at 85 ~ 95 DEG C, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for second extraction, the distilled water of residue 4 ~ 5 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out second time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue is used for three times and extracts, the distilled water of residue 3 ~ 4 times of volumes is added in residue, at 85 ~ 95 DEG C, carry out third time extract 55 ~ 65min, after extraction terminates, centrifugal, revolution is 4000 ~ 5000r/min, centrifugation time 5 ~ 10min, collect supernatant liquor, residue enters next step drying process, merge the supernatant liquor of above-mentioned three times and cross 300 ~ 400 mesh sieves, lentinan is obtained by the filtrate concentrate drying of sieve,
(7) residue is dry: by above-mentioned centrifugal after residue dry under 70 ~ 90 DEG C of conditions, pulverized 60 ~ 80 mesh sieves, can mushroom dietary fiber be obtained.
2. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: in step (3), the screen overflow water content standard of scalping is: when firmly holding with hand, anhydrously to ooze out.
3. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: need at 75 ~ 85 DEG C of concentrating under reduced pressure through the clear liquid of ultra-filtration membrane in step (5), the volume of concentrated solution and the ratio of stoste volume are 1:10 ~ 1:12, then 80 ~ 90 DEG C of oven dry, the mushroom pigment obtained is very easily water-soluble, after dissolving, solution is shiny red, and with strong mushroom fragrance.
4. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: the dope not through ultra-filtration membrane in step (5) need adopt vacuum and low temperature liquid continuous drier to carry out drying, whole drying process, temperature of charge controls between 25 ~ 35 DEG C, vacuum tightness, at ﹣ 0.095 ~ ﹣ 0.099MPa, can guarantee the biological activity of Lentinus Edodes protein like this.
5. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: the solid-to-liquid ratio in step (6) during hot water extraction is the weight in wet base of screen overflow and the ratio of distilled water; As with the weighing scale of dried thin mushroom, to prepare burden in ratio below: the cold weight carrying rear wet feed is about 3 ~ 4 times of siccative weight, add the distilled water of 4 ~ 5 times on this basis again.
6. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: after 300 ~ 400 mesh sieves crossed by the supernatant liquor that in step (6), three times are extracted, concentrating under reduced pressure need be carried out at 80 ~ 90 DEG C, the volume of concentrated solution and the ratio of stoste volume are 1:8 ~ 1:10, then under the condition of inlet temperature 180 ~ 190 DEG C, air outlet temperature 90 ~ 100 DEG C, carry out spraying dry, obtain lentinan powder.
7. the dried thin mushroom that utilizes as described in claim 1 or 6 produces the method for pigment, albumen, polysaccharide and food fibre, it is characterized in that: need spray while hot when lentinan concentrated solution carries out spraying dry.
8. utilize dried thin mushroom to produce the method for pigment, albumen, polysaccharide and food fibre as claimed in claim 1, it is characterized in that: described dried thin mushroom is the various models of booth plantation, the mushroom of various quality, comprises 937 and/or perigone mushroom etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410535782.9A CN104829734B (en) | 2014-10-10 | 2014-10-10 | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410535782.9A CN104829734B (en) | 2014-10-10 | 2014-10-10 | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104829734A true CN104829734A (en) | 2015-08-12 |
| CN104829734B CN104829734B (en) | 2017-06-13 |
Family
ID=53807959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410535782.9A Active CN104829734B (en) | 2014-10-10 | 2014-10-10 | A kind of method of utilization dried thin mushroom production pigment, albumen, polysaccharide and dietary fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104829734B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105399853A (en) * | 2015-12-16 | 2016-03-16 | 黑龙江众生生物工程有限公司 | Production method for extracting various shiitake mushroom effective substances from shiitake mushroom stipe in one time |
| CN106189346A (en) * | 2016-07-08 | 2016-12-07 | 天津工业大学 | A kind of natural brown dyestuff and its preparation method and application |
| CN106520422A (en) * | 2016-10-08 | 2017-03-22 | 福建农林大学 | Anti-aging mushroom concentrated beverage and preparation method thereof |
| CN107373306A (en) * | 2017-07-31 | 2017-11-24 | 广西健美乐食品有限公司 | A kind of preparation method of Russulasp. powder |
| CN107581616A (en) * | 2017-08-18 | 2018-01-16 | 武汉华士特工业生物技术开发有限公司 | A kind of method that protein from lentinus edodes and mushroom oligosaccharide are synchronously prepared using mushroom |
| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
| CN110054708A (en) * | 2019-04-30 | 2019-07-26 | 容益(海南)农业开发有限公司 | A kind of edible mushroom leaching liquor concentrate drying integrated technique |
| CN110483653A (en) * | 2019-07-16 | 2019-11-22 | 南昌大学 | A kind of preparation method and product and application with immunocompetent lentinan component |
| CN114990184A (en) * | 2022-06-16 | 2022-09-02 | 陕西科技大学 | Green co-production preparation method of edible fungus glucan, polypeptide and crude fiber |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533900A (en) * | 2010-12-24 | 2012-07-04 | 河南百禾生源农资有限公司 | Method for extracting lentinan |
| CN102627789A (en) * | 2012-03-31 | 2012-08-08 | 中华全国供销合作总社济南果品研究院 | Preparation process for edible fungus compound polysaccharide |
| CN102757509A (en) * | 2011-04-29 | 2012-10-31 | 北京联合大学生物化学工程学院 | Extraction and purification method for Lentian |
| CN103709265A (en) * | 2013-12-19 | 2014-04-09 | 徐黄毅 | Extraction process for lentinan |
| CN103724447A (en) * | 2013-12-31 | 2014-04-16 | 中国科学院长春应用化学研究所 | Extraction and classification method of lentinan |
| CN103859140A (en) * | 2013-01-28 | 2014-06-18 | 临邑禹王植物蛋白有限公司 | Technology for extracting isolated soybean protein at low temperature |
-
2014
- 2014-10-10 CN CN201410535782.9A patent/CN104829734B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533900A (en) * | 2010-12-24 | 2012-07-04 | 河南百禾生源农资有限公司 | Method for extracting lentinan |
| CN102757509A (en) * | 2011-04-29 | 2012-10-31 | 北京联合大学生物化学工程学院 | Extraction and purification method for Lentian |
| CN102627789A (en) * | 2012-03-31 | 2012-08-08 | 中华全国供销合作总社济南果品研究院 | Preparation process for edible fungus compound polysaccharide |
| CN103859140A (en) * | 2013-01-28 | 2014-06-18 | 临邑禹王植物蛋白有限公司 | Technology for extracting isolated soybean protein at low temperature |
| CN103709265A (en) * | 2013-12-19 | 2014-04-09 | 徐黄毅 | Extraction process for lentinan |
| CN103724447A (en) * | 2013-12-31 | 2014-04-16 | 中国科学院长春应用化学研究所 | Extraction and classification method of lentinan |
Non-Patent Citations (1)
| Title |
|---|
| 李波等: "香菇蛋白的提取制备方法研究", 《食品工业科技》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105399853A (en) * | 2015-12-16 | 2016-03-16 | 黑龙江众生生物工程有限公司 | Production method for extracting various shiitake mushroom effective substances from shiitake mushroom stipe in one time |
| CN106189346A (en) * | 2016-07-08 | 2016-12-07 | 天津工业大学 | A kind of natural brown dyestuff and its preparation method and application |
| CN109206938A (en) * | 2016-07-08 | 2019-01-15 | 天津工业大学 | A kind of natural brown dyestuff |
| CN109206938B (en) * | 2016-07-08 | 2019-10-15 | 天津工业大学 | A kind of natural brown dyestuff |
| CN106520422A (en) * | 2016-10-08 | 2017-03-22 | 福建农林大学 | Anti-aging mushroom concentrated beverage and preparation method thereof |
| CN107373306A (en) * | 2017-07-31 | 2017-11-24 | 广西健美乐食品有限公司 | A kind of preparation method of Russulasp. powder |
| CN107581616A (en) * | 2017-08-18 | 2018-01-16 | 武汉华士特工业生物技术开发有限公司 | A kind of method that protein from lentinus edodes and mushroom oligosaccharide are synchronously prepared using mushroom |
| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
| CN110054708A (en) * | 2019-04-30 | 2019-07-26 | 容益(海南)农业开发有限公司 | A kind of edible mushroom leaching liquor concentrate drying integrated technique |
| CN110483653A (en) * | 2019-07-16 | 2019-11-22 | 南昌大学 | A kind of preparation method and product and application with immunocompetent lentinan component |
| CN110483653B (en) * | 2019-07-16 | 2022-03-18 | 南昌大学 | Preparation method of lentinan component with immunocompetence, product and application |
| CN114990184A (en) * | 2022-06-16 | 2022-09-02 | 陕西科技大学 | Green co-production preparation method of edible fungus glucan, polypeptide and crude fiber |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104829734B (en) | 2017-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104829734A (en) | Method of producing pigment, protein, polysaccharide and dietary fiber from dry lentinula edodes | |
| CN103843970B (en) | Production method for preparing ossein oligopeptide meal, bone oil and bone meal | |
| CN108085356A (en) | Using cold pressing walnut dregs as the method for primary industry metaplasia production of high purity walnut peptide | |
| CN107858393A (en) | A kind of method that polypeptide is extracted from walnut dregs | |
| CN103394071B (en) | Method for producing giant salamander polypeptide powder liver protection capsules | |
| CN102994598B (en) | Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof | |
| CN101731615A (en) | Preparation method of clam nutrition health care condiment | |
| CN102696989A (en) | Manufacture method of natural coconut powder | |
| CN102643368A (en) | Method for synchronously extracting taurine, chitin and polypeptide from shrimp heads and shrimp leftovers | |
| CN105061382B (en) | Method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin | |
| CN106962590A (en) | A kind of method for preparing edible fungus protein powder | |
| CN102406048A (en) | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution | |
| CN104016854A (en) | Calcium citrate prepared from abalone shell as raw material and preparation method of calcium citrate | |
| CN104304480A (en) | Fast dissolving ginkgo nut milk tea and preparation method thereof | |
| CN103725031A (en) | Process for extracting purplish red pigment from dried red jujube skin | |
| CN104892780A (en) | Extraction process of jujube residue crude polysaccharide | |
| CN109619264A (en) | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide | |
| CN103864956B (en) | A kind of method extracting pectin from eggplant fruit | |
| CN105367680A (en) | Microwave-assisted extraction method for auricularia auriculajudae polysaccharides | |
| CN108244329B (en) | Preparation process of quinoa germ protein powder | |
| CN103980384A (en) | Preparation method of water-soluble pectin of shaddock albedo | |
| CN104926957B (en) | Method of extracting polysaccharides from Pleurotus eryngii | |
| CN107893095A (en) | A kind of method that marine source albumen prepares high F value oligopeptide | |
| CN107417804A (en) | Edible fungi polysaccharide extracting method | |
| CN108125209B (en) | Black rice anthocyanin salt with antioxidant effect and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240510 Address after: 112611 Balizhuang village, xintaizi Town, Tieling County, Tieling City, Liaoning Province Patentee after: LIAONING COMPLETE BIO-TECHNOLOGY Co.,Ltd. Country or region after: China Address before: Inside the First Grain Depot, No. 31 Beiyi Middle Road, Tiexi District, Shenyang City, Liaoning Province, 110025 Patentee before: SHENYANG GRAIN AND OIL FOOD SCIENCE INSTITUTE Country or region before: China |
|
| TR01 | Transfer of patent right |