CN107858393A - A kind of method that polypeptide is extracted from walnut dregs - Google Patents

A kind of method that polypeptide is extracted from walnut dregs Download PDF

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CN107858393A
CN107858393A CN201711330108.7A CN201711330108A CN107858393A CN 107858393 A CN107858393 A CN 107858393A CN 201711330108 A CN201711330108 A CN 201711330108A CN 107858393 A CN107858393 A CN 107858393A
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walnut
dregs
polypeptide
protein
walnut dregs
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CN107858393B (en
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缪福俊
宁德鲁
王洋
肖良俊
马婷
李勇杰
耿树香
陈海云
吴涛
贺娜
张艳丽
徐田
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Yunnan Academy of Forestry
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

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Abstract

The present invention relates to a kind of method that polypeptide is extracted from walnut dregs, comprise the following steps：Step（1）, raw material pretreatment；Step（2）, alkali soluble and enzymolysis；Step（3）, high temperature enzyme deactivation；Step（4）, separation of solid and liquid；Step（5）, filtration drying.Present invention process is simple and economical, and protein extraction and polypeptide are prepared and carried out simultaneously, are effectively shortened the time of protein extraction and polypeptide preparation, and the recovery rate of albumen and polypeptide conversion ratio further improve；Production process is green, and product possesses the functions such as preferable antioxidation activity, decompression, may be directly applied to the fields such as functional food, medicine, cosmetics；Material source is extensive, cheap, can turn waste into wealth, and improves the attached value of walnut dregs.

Description

A kind of method that polypeptide is extracted from walnut dregs
Technical field
The present invention relates to a kind of method for extracting polypeptide, especially a kind of side that polypeptide is extracted from walnut dregs Method, food processing field.
Background technology
China's walnut cultivated area and yield rank first in the world, and Yunnan is first of the whole nation again, ends 2015, Yunnan Province 42,300,000 mu of walnut cultivated area, 850,000 tons of yield, yield, area are the first in the nation.With walnut yield more and more higher, mesh Before, domestic walnut oil expression manufacturer is more and more, produces the substantial amounts of degreasing walnut dregs of rice therewith.Walnut dregs are often by as feed It is relatively low with fertilizer or discarding, waste discharge pollution environment, its attached value.Research shows, by the walnut dregs after cold press de-oiling Protein content is up to 50%, is high-quality protein resource, and DEVELOPMENT PROSPECT is wide.
Scientific research in recent years finds that human consumption's protein is after digesting enzyme effect, and the non-principal shape with amino acid Formula absorbs, but is absorbed in the form of peptide, and some peptides can not only provide growth in humans, development needed nutrient matter, have simultaneously Different physiological roles, if improving mineral matter transport and absorption, antibacterial and virus, improving immunity, be anti-oxidant, drop courage Sterol, antitumaous effect, remove the anti-ageing effect of waiting for a long time of free radical.Therefore, walnut is prepared using albumen in protease hydrolytic walnut dregs Gly-His-Lys, realize the purpose that its attached value is improved using walnut dregs.Meanwhile obtained walnut peptide powder have strengthen immunity, Antioxidation activity, the function such as it is depressured, sets up.
At present, existing walnut（Benevolence/the dregs of rice）Polypeptide technology of preparing mainly has two classes, and one kind is by walnut（Benevolence/the dregs of rice） Raw material is digested after directly carrying out defibrination, and protein extracting ratio is relatively low；Another kind of is by walnut（Benevolence/the dregs of rice）Pass through after being crushed The heavy method of alkali soluble acid first extracts protein, is digested after adjusting pH value further according to enzyme viability, complex process.Due to walnut Also contain 10 ~ 15% greases in the dregs of rice, when this two classes method carries out protein extraction using walnut dregs raw material, emulsion is serious, greatly Ground limits the dissolution of albumen in walnut dregs so that protein extracting ratio and purity of protein are relatively low, corresponding polypeptide conversion ratio It is relatively low, add production cost.It is therefore desirable to prior art is improved.
The content of the invention
To solve, protein extracting ratio and purity of protein be not high in existing walnut camellia meal protein polypeptide preparation process, emulsion is tight Weight, the problems such as more blr polypeptides are low, polypeptide preparation technology complexity, it is more that albumen is extracted from walnut dregs the invention provides one kind The method of peptide, can rapid extraction prepare polypeptide.
The technical solution adopted for the present invention to solve the technical problems is as follows：
A kind of method that polypeptide is extracted from walnut dregs, comprises the following steps：
Step（1）, raw material pretreatment：Walnut dregs raw material is pulverized and sieved, obtains walnut dregs crushed material, then is mixed with water, is obtained To walnut dregs mixed liquor, non-protein complex enzyme is added into mixed liquor, carries out enzyme hydrolysis, the time is 2~5h, and enzyme hydrolysis temperature is 30~40 DEG C, mixing speed is 30~60r/min, whole process stirring, and the suspension of walnut dregs broken wall is made；
Step（2）, alkali soluble and enzymolysis：To step（1）Suspension in add alkali lye, regulation pH is 8.0~10.0, is added simultaneously The alkali protease of 0.2~2% walnut dregs crushed material quality so that alkali soluble and enzymolysis are carried out simultaneously, and the reaction time is 3~10h, Reaction temperature is 40~60 DEG C, and pH keeps 8.0~10.0, and mixing speed is 30~60r/min, whole process stirring, walnut dregs is made Enzymolysis liquid；
Step（3）, high temperature enzyme deactivation：By step（3）Walnut dregs enzymolysis liquid temperature rise to 90~95 DEG C, high temperature enzyme deactivation 5~ 10min, obtain the walnut dregs enzymolysis liquid through enzyme deactivation；
Step（4）, solid- liquid separation：By step（3）Enzymolysis liquid carry out separation of solid and liquid, centrifugation 10 under 5000~8000r/min~ 20min, supernatant is taken, adjust pH that walnut protein peptide liquid is made to neutrality；
Step（5）, filtration drying：To step（4）Walnut protein peptide liquid carry out concentrated spray drying, obtain walnut protein polypeptide Powder；Or step is retained using regenerated cellulose film（4）Walnut protein peptide liquid, obtain molecular weight be 1~5kD it is oligomeric more Peptide liquid, concentrated spray is then carried out again and is dried to obtain walnut oligopeptide or small molecule Gly-His-Lys.
Further, step（1）In, walnut dregs raw material is the dregs of rice of the walnut after hydraulic oil press squeezes, protein content >= 50%, 40~100 mesh sieves are crossed after crushing, then by walnut dregs crushed material and water by weight 1:After 5~10 ratio is well mixed, Obtain walnut dregs mixed liquor.
Further, step（1）In, non-protein complex enzyme is cellulase, pectase and amylase in parts by weight 1~5： 0.5~4：0.5~5 composition, the addition of non-protein complex enzyme are the 0.05~1.00% of walnut dregs crushed material quality, enzymolysis Conditions Temperature is 30~40 DEG C；
Further, step（2）In, alkali soluble is NaOH or Ca (OH)2Solution；
Further, step（5）In, peptide liquid is first concentrated in vacuo to the 1/5 ~ 1/3 of stoste volume at 40~60 DEG C, then will concentration Liquid is spray-dried, and leaving air temp is 50 DEG C~80 DEG C, and EAT is 120 DEG C~180 DEG C, and it is more to obtain walnut after drying Gly-His-Lys.
Plant cell wall is mainly made up of polysaccharose substances such as pectin and celluloses, and protein is wrapped in wherein, this Shen Please method can effectively disintegrate cell membrane, promote the dissolution of intracellular protein.Non-protein enzyme（Cellulase, pectase, starch Enzyme etc.）Can be with the cellulose skeleton of degrading plant cell membrane, collapse cell membrane, can also be by starch kind material insoluble in raw material Matter, cellulosic material, the solable matter that pectic substance enzymolysis is short chain, so as to reach what is separated with protein raw materials in raw material Purpose, and protease can then be obviously improved emulsion, increase the recovery rate and purity of protein of albumen.
Compared with prior art, beneficial effects of the present invention and advantage are as follows：
（1）Turn waste into wealth, improve the attached value of walnut dregs.Waste material-walnut dregs after walnut squeezing liquefaction are taken full advantage of as former Expect the high protein resource being rich in the dregs of rice, solve the degradation problem of walnut protein, can be by the nutrient protein in the degreasing walnut dregs of rice Ingredient degradation, it can be walnut peptide powder that human body directly absorbs rapidly, nutritious to be hydrolyzed into it, have enhancing human immunity Power, anti-aging, anti-oxidant, the function such as set up.The method of present invention production walnut peptide powder, the attached valency of walnut dregs can be improved Value, implementing process is easy and economical, is adapted to scale industrial production.
（2）Production process is green, and the recovery rate and purity of albumen further improve.Wherein, amylase, cellulase Joint enzymolysis is carried out with three kinds of non-protein enzymes of pectase, not only can effectively degradation of cell wall so that walnut dregs powder raw material is thin Albumen in born of the same parents is fully dissolved out, and highly shortened the extraction time of walnut protein, can also be by starch insoluble in raw material Class material, cellulosic material, pectic substance enzymolysis for short chain solable matter, so as to reach and protein raw materials in raw material point The purpose opened, further improve the recovery rate and purity of albumen, and do not produce pollution in production process, it is with short production cycle, into Sheet is low, does not produce any poisonous and harmful substance, green safe, has no toxic side effect, the polypeptide powder of preparation may be directly applied to The fields such as functional food, medicine, cosmetics.
（3）Walnut camellia meal protein polypeptide extraction and preparation technique simplifies, and protein extraction and polypeptide are prepared and carried out simultaneously.In alkali soluble During, it is directly added into alkali protease and carries out enzymatic hydrolysis reaction, and alkali protease effectively improves emulsion, increases Add the recovery rate and purity of protein of albumen.Integrated artistic avoids the heavy process of acid, compared with the heavy extraction of acid again of existing first alkali soluble After protein, then adjust pH to carry out enzymatic hydrolysis reaction, greatly simplifie walnut protein extraction enzymolysis process, shorten protein extraction The time prepared with polypeptide, effectively increase the recovery rate of walnut cake protein（≥93%）And purity（≥88%）, and make albumen The conversion ratio that matter is degraded to peptide improves, up to more than 85%.Protein extracting ratio of the present invention, purity of protein, polypeptide conversion ratio are far above Index of correlation of the prior art.
（4）Product possesses the functions such as preferable antioxidation activity, decompression.Walnut small molecule Gly-His-Lys, molecule can be made in the present invention Amount is less than 2000 dalton, OH clearance rate >=70%, oxygen radical removing rate >=68%, DPPH clearance rate >=83%, possess compared with Strong antioxidation activity；ACE inhibiting rates >=73% antihypertensive effect is notable.
Embodiment
It is clearly and completely described below in conjunction with the technical scheme in embodiment, it is clear that described embodiment is only Only it is to a part of example of the present invention, rather than whole examples.Based on the embodiment in the present invention, ordinary skill people The every other embodiment that member is obtained under the premise of creative work is not paid, belongs to the scope of protection of the invention.
Embodiment 1
Method prepared by the walnut protein polypeptide powder extraction of the present embodiment, comprises the following steps：
Step（1）, raw material pretreatment：Walnut dregs raw material is crushed with pulverizer, and crosses 60 mesh sieves, obtains walnut dregs crushing Material, then with water by weight 1：5 ratio mixing, obtains walnut dregs mixed liquor, and non-protein complex enzyme is added into mixed liquor, non- Albumen complex enzyme is cellulase, pectase and amylase by proportioning 5：2：3 compositions, the addition of non-protein complex enzyme is walnut The 0.1% of crushed material quality, reaction temperature are 35 DEG C, reaction time 3h, whole process stirring, mixing speed 30r/min, core are made The suspension of peach dregs of rice broken wall；
Step（2）, alkali soluble and enzymolysis：Above-mentioned suspension is added into retort, Ca (OH) is added into above-mentioned suspension2Solution, The pH to 8.0 of suspension is adjusted, adds 0.2% alkali protease of walnut crushed material quality, enzyme activity is 300,000 U/g, is entered The dissolution of row albumen and protease hydrolytic, reaction time 6h, reaction temperature are 50 DEG C, and pH value keeps constant, whole process stirring, stirring speed Spend for 60r/min, obtained walnut dregs enzymolysis liquid；
Step（3）, high temperature enzyme deactivation：Temperature in retort is risen to 90 DEG C, high temperature enzyme deactivation 8min；
Step（4）, solid- liquid separation：By step（3）Enzyme deactivation enzymolysis liquid separation of solid and liquid, 5000r/min centrifugation 10min, remove Insoluble solid, supernatant is taken, obtain walnut protein peptide liquid；
Step（5）, be concentrated and dried：Above-mentioned walnut protein peptide liquid is concentrated in vacuo to the 1/5 of stoste volume at 50 DEG C, then will be dense Contracting liquid is spray-dried, and leaving air temp is 70 DEG C, and EAT is 150 DEG C, and walnut protein polypeptide powder is obtained after drying.
Embodiment 2
The walnut polypeptide powder extraction preparation method of the present embodiment, comprises the following steps：
Step（2）, raw material pretreatment：Walnut dregs raw material is crushed with pulverizer, and crosses 60 mesh sieves, obtains walnut dregs crushing Material, then with water by weight 1：6 ratio mixing, obtains walnut dregs mixed liquor, and non-protein complex enzyme is added into mixed liquor, non- Albumen complex enzyme is cellulase, pectase and amylase by proportioning 4.5：2：3.5 compositions, the addition of non-protein complex enzyme are The 0.2% of walnut crushed material quality, reaction temperature are 37 DEG C, reaction time 4h, whole process stirring, mixing speed 30r/min, system Obtain the suspension of walnut dregs broken wall；
Step（2）, alkali soluble and enzymolysis：Above-mentioned suspension is added into retort, NaOH solution is added into above-mentioned suspension, is adjusted The pH of suspension to 8.5,0.3% alkali protease of walnut crushed material quality is added, enzyme activity is 300,000 U/g, carries out egg White dissolution and protease hydrolytic, reaction time 7h, reaction temperature are 52 DEG C, and pH value keeps constant, whole process stirring, and mixing speed is 60r/min, walnut dregs enzymolysis liquid is made；
Step（3）, high temperature enzyme deactivation：Retort temperature is risen to 92 DEG C, high temperature enzyme deactivation 7min；
Step（4）, solid- liquid separation：By step（3）Enzyme deactivation enzymolysis liquid carry out separation of solid and liquid, 6000r/min centrifugation 5min, Insoluble solid is removed, takes supernatant, walnut protein peptide liquid is made；
Step（5）, filtration drying：It is less than the polypeptide liquid of 5000 dalton using regenerated cellulose retaining molecular weight, then 55 The 1/4 of stoste volume is concentrated in vacuo at DEG C, then concentrate is spray-dried, leaving air temp is 75 DEG C, and EAT is 155 DEG C, walnut polypeptide powder is obtained after drying.
Embodiment 3
The walnut polypeptide powder extraction preparation method of the present embodiment, comprises the following steps：
Step（1）, raw material pretreatment：Walnut dregs raw material is crushed with pulverizer, and crosses 60 mesh sieves, obtains obtaining walnut dregs crushing Material, then with water by weight 1：Walnut dregs mixed liquor is made after 7 ratio mixing, non-protein complex enzyme is added into mixed liquor, Non-protein complex enzyme is cellulase, pectase and amylase by proportioning 5：1.5：3.5 compositions, the addition of non-protein complex enzyme For the 0.3% of walnut crushed material quality, enzyme hydrolysis temperature is 40 DEG C, enzyme hydrolysis time 5h, whole process stirring, mixing speed 30r/ Min, the suspension of walnut dregs broken wall is made；
Step（2）, alkali soluble and enzymolysis：Above-mentioned suspension is added into retort, NaOH solution is added into above-mentioned suspension, is adjusted The pH of suspension to 9.0, the alkali protease of 0.25% walnut crushed material quality is added, enzyme activity is 300,000 U/g, carries out egg White dissolution and protease hydrolytic, reaction time 8h, reaction temperature are 54 DEG C, and pH value keeps constant, whole process stirring, and mixing speed is 60r/min, walnut dregs enzymolysis liquid is made；
Step（3）, high temperature enzyme deactivation：Retort temperature is risen to 95 DEG C, high temperature enzyme deactivation 5min；
Step（4）, solid- liquid separation：By step（3）Enzyme deactivation enzymolysis liquid carry out separation of solid and liquid, 5000r/min centrifugation 12min, Insoluble solid is removed, takes supernatant, walnut protein peptide liquid is made；
Step（5）, filtration drying：
Step is retained using regenerated cellulose film（4）Walnut protein peptide liquid, obtain molecular weight be less than 2000 dalton polypeptide Liquid, the 1/3 of stoste volume then being concentrated in vacuo at 60 DEG C, then concentrate being spray-dried, leaving air temp is 70 DEG C, is entered Air temperature is 170 DEG C, and Walnut low molecular weight polypeptide powder is obtained after drying.
The Walnut low molecular weight polypeptide powder that the method for embodiment 3 obtains is subjected to antioxidation activity and ACE inhibiting rates are detected Analysis, as a result as shown in table 1：
The antioxidation activity of table 1 and ACE inhibiting rate results
Found out by table 1, OH clearance rate >=70% of micromolecule polypeptide powder, oxygen radical removing rate >=68%, DPPH clearance rates >=83%, possess stronger antioxidation activity, ACE inhibiting rates >=73% antihypertensive effect is notable.
Protein extracting ratio in the inventive method, purity, polypeptide conversion ratio are tested and analyzed, and to prior art side Method has also carried out Experimental Comparison detection and analysis, and treatment group is all digested using alkali protease, as a result as shown in table 2.
The protein extracting ratio of the prior art of table 2 and the application, purity, the contrast of polypeptide conversion ratio
As can be seen from Table 2, protein extracting ratio of the present invention, purity of protein, polypeptide conversion ratio are surveyed far above art methods The index of correlation obtained.The conversion that the recovery rate of walnut cake protein of the present invention is up to 93%, high purity 88%, protein degradation is peptide Rate is up to 85%, significant effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.

Claims (5)

  1. A kind of 1. method that polypeptide is extracted from walnut dregs, it is characterised in that：
    Comprise the following steps：
    Step（1）, raw material pretreatment：Walnut dregs raw material is pulverized and sieved, obtains walnut dregs crushed material, then is mixed with water, is obtained To walnut dregs mixed liquor, non-protein complex enzyme is added into mixed liquor, carries out enzyme hydrolysis, the time is 2~5h, and enzyme hydrolysis temperature is 30~40 DEG C, mixing speed is 30~60r/min, whole process stirring, and the suspension of walnut dregs broken wall is made；
    Step（2）, alkali soluble and enzymolysis：To step（1）Suspension in add alkali lye, regulation pH is 8.0~10.0, is added simultaneously The alkali protease of 0.2~2% walnut dregs crushed material quality so that alkali soluble and enzymolysis are carried out simultaneously, and the reaction time is 3~10h, Reaction temperature is 40~60 DEG C, and pH keeps 8.0~10.0, and mixing speed is 30~60r/min, whole process stirring, walnut dregs is made Enzymolysis liquid；
    Step（3）, high temperature enzyme deactivation：By step（3）Walnut dregs enzymolysis liquid temperature rise to 90~95 DEG C, high temperature enzyme deactivation 5~ 10min, obtain the walnut dregs enzymolysis liquid through enzyme deactivation；
    Step（4）, solid- liquid separation：By step（3）Enzymolysis liquid carry out separation of solid and liquid, centrifugation 10 under 5000~8000r/min~ 20min, supernatant is taken, adjust pH that walnut protein peptide liquid is made to neutrality；
    Step（5）, filtration drying：To step（4）Walnut protein peptide liquid carry out concentrated spray drying, obtain walnut protein polypeptide Powder；Or step is retained using regenerated cellulose film（4）Walnut protein peptide liquid, obtain molecular weight be 1~5kD it is oligomeric more Peptide liquid, concentrated spray is then carried out again and is dried to obtain walnut oligopeptide or small molecule Gly-His-Lys.
  2. 2. the method according to claim 1 that polypeptide is extracted from walnut dregs, it is characterised in that：Step（1）In, core Peach dregs of rice raw material is the dregs of rice of the walnut after hydraulic oil press squeezes, protein content >=50%, crosses 40~100 mesh sieves after crushing, then by core Peach dregs of rice crushed material is with water by weight 1:After 5~10 ratio is well mixed, walnut dregs mixed liquor is obtained.
  3. 3. the method according to claim 1 that polypeptide is extracted from walnut dregs, it is characterised in that：Step（1）In, it is non- Albumen complex enzyme is cellulase, pectase and amylase in parts by weight 1~5：0.5~4：0.5~5 composition, non-protein are multiple The addition of synthase is the 0.05~1.00% of walnut dregs crushed material quality, and enzymatic hydrolysis condition temperature is 30~40 DEG C.
  4. 4. the method according to claim 1 that polypeptide is extracted from walnut dregs, it is characterised in that step（2）In, alkali Molten is NaOH or Ca (OH)2Solution.
  5. 5. the method according to claim 1 that polypeptide is extracted from walnut dregs, it is characterised in that step（5）In, peptide Liquid is first concentrated in vacuo to the 1/5 ~ 1/3 of stoste volume at 40~60 DEG C, then concentrate is spray-dried, and leaving air temp is 50 DEG C~80 DEG C, EAT is 120 DEG C~180 DEG C, and walnut polypeptide powder is obtained after drying.
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Cited By (10)

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Publication number Priority date Publication date Assignee Title
CN108517342A (en) * 2018-04-24 2018-09-11 陆新军 A kind of method that more algae proteolysis prepare polypeptide
CN108546281A (en) * 2018-04-26 2018-09-18 九江牧威利元科技中心（普通合伙） A kind of ginseng oligopeptide and its preparation method and application
CN109527161A (en) * 2018-11-21 2019-03-29 云南省林业科学院 A kind of walnut protein instant coffee and preparation method thereof
CN109609576A (en) * 2018-12-29 2019-04-12 浙江工业大学 A kind of extracting method of hickory nut polypeptide
CN110089665A (en) * 2019-05-14 2019-08-06 西安中粮工程研究设计院有限公司 A kind of decolourize for walnut protein peptide bitter composition and method
CN110438190A (en) * 2019-09-12 2019-11-12 北京瓜尔润科技股份有限公司 A kind of method and Guar active peptide using a variety of enzyme complex enzyme hydrolysis preparation Guar active peptide
CN110710592A (en) * 2019-11-16 2020-01-21 四川农业大学 Method for improving antioxidant activity of walnut cake protein
CN112795611A (en) * 2021-01-25 2021-05-14 昆明生物制造研究院有限公司 Method for preparing walnut protein polypeptide from insoluble protein
CN114668152A (en) * 2022-03-14 2022-06-28 北京林业大学 ACE inhibitory peptide liposome with high stability and directional release in small intestine and preparation method thereof
CN117778509B (en) * 2024-01-04 2024-06-07 吉林肽谷生物工程有限责任公司 Walnut peptide extraction method

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CN104172413A (en) * 2014-08-07 2014-12-03 陕西天玉实业有限公司 Preparation method of walnut polypeptide beverage

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WO2009042622A2 (en) * 2007-09-25 2009-04-02 Novozymes A/S Process for the production of a fermentation product from a wood-containing material, wherein the wood-containing material is treated with esterases
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CN104172413A (en) * 2014-08-07 2014-12-03 陕西天玉实业有限公司 Preparation method of walnut polypeptide beverage

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108517342A (en) * 2018-04-24 2018-09-11 陆新军 A kind of method that more algae proteolysis prepare polypeptide
CN108546281A (en) * 2018-04-26 2018-09-18 九江牧威利元科技中心（普通合伙） A kind of ginseng oligopeptide and its preparation method and application
CN109527161A (en) * 2018-11-21 2019-03-29 云南省林业科学院 A kind of walnut protein instant coffee and preparation method thereof
CN109609576A (en) * 2018-12-29 2019-04-12 浙江工业大学 A kind of extracting method of hickory nut polypeptide
CN110089665A (en) * 2019-05-14 2019-08-06 西安中粮工程研究设计院有限公司 A kind of decolourize for walnut protein peptide bitter composition and method
CN110438190A (en) * 2019-09-12 2019-11-12 北京瓜尔润科技股份有限公司 A kind of method and Guar active peptide using a variety of enzyme complex enzyme hydrolysis preparation Guar active peptide
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CN110710592A (en) * 2019-11-16 2020-01-21 四川农业大学 Method for improving antioxidant activity of walnut cake protein
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