CN109651480A - A method of separation momordica glycoside V - Google Patents
A method of separation momordica glycoside V Download PDFInfo
- Publication number
- CN109651480A CN109651480A CN201811397385.4A CN201811397385A CN109651480A CN 109651480 A CN109651480 A CN 109651480A CN 201811397385 A CN201811397385 A CN 201811397385A CN 109651480 A CN109651480 A CN 109651480A
- Authority
- CN
- China
- Prior art keywords
- momordica glycoside
- enzyme
- separation
- resin
- glycoside
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to active ingredients of plants extraction purification fields, are especially to provide a kind of method that momordica glycoside V is separated from Siraitia grosvenorii.A method of separation momordica glycoside V, the method is that plant enzyme enzymatic hydrolysis, biological enzyme enzymatic hydrolysis are successively carried out after Siraitia grosvenorii to be added to water mashing, enzymolysis liquid is collected, yeast fermentation is carried out after enzyme deactivation, momordica glycoside V is made in compound anion-cation exchange resin purifying, concentrate drying.The product product that this production method is come is snow-white, and no bitter taste, dissolvent residual is few, non agricultural chemical residuum, product with stable quality, and gained momordica glycoside V is greater than 60%, and total glycosides is greater than 90%.
Description
Technical field
The present invention relates to active ingredients of plants extraction purification fields, are especially to provide one kind and separate Siraitia grosvenorii from Siraitia grosvenorii
The method of sweet tea glycosides V.
Background technique
Siraitia grosvenorii is described as " angle fruit " by people, is the distinctive precious cucurbitaceous plant in China, be known as the good fruit of good medicine it
Claim.With diseases such as clearing heat and moistening lung, cough-relieving, relieving sore-throats.The main component of Fructus Monordicae extract is mogroside.In mogroside
Effective component be mainly terpenoid, including glycosides III, glycosides IV, glycosides V etc., wherein mogroside V is the master of its sweet taste
Ingredient is wanted, sugariness is 256~344 times of sucrose, since it is combined with glycosyl and soluble easily in water, ethyl alcohol isopolarity solvent, indissoluble
It is white amorphous powder in organic solvents such as acetone, ethyl acetate.It can be widely applied to nutrient and healthcare products, food, drug etc.
In industry.
Currently, the extracting method about Fructus Monordicae extract mainly has water to mention, alcohol extracting method, it is subsequent to use macroporous absorbent resin
And ion exchange resin is purified, but extraction process medium temperature spends the high impurity that will increase leaching, the color of extracting solution adds
It is deep, and it is unfavorable for subsequent decolorization and purification treatment, and obtained extract color is deeply and with strong taste of traditional Chinese medicine and by force
Strong rear bitter taste, the shortcomings that active constituent content is low, energy consumption is big, high production cost.
Summary of the invention
In place of solving above-mentioned the deficiencies in the prior art, provides and a kind of separate momordica glycoside V from Siraitia grosvenorii
Method, this method is easy to operate, process economics are environmentally friendly, is suitble to promote, gained momordica glycoside V is with high purity, appearance condition is good,
Good taste is without bitter taste.
The purpose of the present invention is achieved through the following technical solutions:
A method of separation momordica glycoside V, the method are successively to carry out plant after Siraitia grosvenorii to be added to water mashing
Enzyme digests, biological enzyme digests, and collects enzymolysis liquid, yeast fermentation is carried out after enzyme deactivation, compound anion-cation exchange resin purifies, is dense
The dry obtained momordica glycoside V of contracting；
The plant enzyme be cellulase, hemicellulase, 1,4 beta-glucanase, pectase or cellulolytic enzyme in extremely
Few one kind；
The biological enzyme be pectin lyase, papain, acid protease, amylase or paragalactan enzyme in extremely
Few one kind.
Preferably, in terms of kg/mL, the Siraitia grosvenorii and plant enzyme mass volume ratio are 1: 0.5~3.
Preferably, the plant enzyme enzymatic hydrolysis is that 1~5 is digested under the conditions of PH is 4.0~4.8, temperature is 40~45 DEG C
Hour.
Preferably, in terms of kg/mL, the Siraitia grosvenorii and biological enzyme mass volume ratio are 1: 0.5~15.
Preferably, the biological enzyme hydrolysis temperature is 45~50 DEG C, and enzymolysis time is 1~3 hour.
Preferably, the enzyme-removal temperature is 80~85 DEG C, and the enzyme deactivation time is 5~10 minutes.
Preferably, in terms of kg/g, the Siraitia grosvenorii and yeast mass ratio are 1: 0.5~5；The yeast fermentation temperature is
25~30 DEG C, fermentation time is 3~5 hours.
It preferably, further include organic membrane filter step, institute before the compound anion-cation exchange resin purification step
Stating organic membrane filter step includes that filtering for the first time and second filter, and the first time filtering uses 5000 molecular weight of retention
Organic film, filter pressure are 0.45~0.5MPa；Organic film of second of the filtering using 400 molecular weight of retention, filtration pressure
Power is 1.8~2.0MPa.
Preferably, in the compound anion-cation exchange resin resin anion (R.A.) and resin cation mass ratio be 1~
3:1~1.5；Compound anion-cation exchange resin purifying eluent used is water.
Preferably, concentrate concentration is 15~18 Baume degrees in the concentration step.
The present invention has the beneficial effect that:
1, the present invention is extracted using using the traditional hot water of enzymatic isolation method extraction substitution, by the knot for destroying Siraitia grosvenorii cell wall
Structure, enables momordica glycoside V sufficient separate out from Siraitia grosvenorii, and the extracting solution impurity extracted is few.
2, the substance decompositions such as the middle polysaccharide of Siraitia grosvenorii, albumen are before small molecule list using organized enzyme by present invention fermentation
The organic matters such as sugar, amino acid, yeast can directly use these small-molecule substances, make to greatly shorten the time required to fermenting step.
Yeast fermentation of the present invention is carried out in aerobic conditions, and the product of yeast aerobic fermentation is carbon dioxide and water, will not be introduced new
Impurity, the present invention yeast fermentation before carry out destroy the enzyme treatment, avoid the effect of enzyme that yeast cell wall is made to be damaged and break
It splits, and then guarantees the recovery rate and purity of momordica glycoside V.
3, combining with fermentation is digested, in addition film decoloration substitutes traditional macroporous resin purification, and purer than its (macroreticular resin)
The momordica glycoside V content of change is high by 10% or more.
4, organic membrane filter liquid passes through compound zwitterion adsorption and desorption by resin, and efflux is limpid clean, and non-variegation passes through
After spray drying, snow-white, uniform, the product of pure taste disposably can be obtained by.
5, this method whole process only uses aqueous solvent, and toxic organic reagent is not used in environmental protection, easy to operate, is well suited for work
Sparetime university's production.
6, the product product that this production method is come is snow-white, and no bitter taste, dissolvent residual is few, non agricultural chemical residuum, and product quality is steady
Fixed, gained momordica glycoside V content is greater than 60%, and total glycosides (contained methods of glycosides in Siraitia grosvenorii) content is greater than 90%.
Specific embodiment
Combined with specific embodiments below, further details of elaboration is made to the present invention, but embodiments of the present invention are not
It is confined to the range of embodiment expression.These embodiments are merely to illustrate the present invention, range and is not intended to limit the present invention.This
Outside, after reading the contents of the present invention, those skilled in the art can various modifications may be made to the present invention, these equivalent variations are same
Sample falls within the appended claims limited range of the present invention.
Embodiment 1
Mature, fresh Siraitia grosvenorii 1kg is taken, is crushed, 3kg pure water is added to be beaten with beater.PH is adjusted with 3% citric acid to arrive
4.0, cellulase 0.5ml is first added, is digested 1 hour at 40 DEG C.By 100 mesh filter clothes be further continued for be added pectin lyase 3ml,
It is small to digest 1 at a temperature of 45 DEG C for papain 3ml, acid protease 3ml, amylase 3ml, paragalactan enzyme 3ml totally five kinds of enzymes
When, enzymolysis liquid is obtained, enzymolysis liquid is heated to 80 DEG C, keeps progress enzyme deactivation in 5 minutes, lets cool to 25 DEG C, and high-activity yeast powder is added
0.5g (was purchased from Anqi Yeast (Chongzuo) Co., Ltd.), at ferment under aerobic conditions 3 hours.Fermentation liquid is crossed into organic film, first mistake
The organic film of 5000 molecular weight obtains de- after the organic film of 400 molecular weight into film pressure 1.8MPa into film pressure 0.45MPa
Color crosses film liquid；The compound zwitterion resin column that film liquid is directly 200g into resin loading is crossed, wherein model D301R (weak base
Resin anion (R.A.)) and D152 (low-acid cationic resin) mass ratio be 1:1 (anions and canons resin is purchased from Tianjin Nankai tree
Rouge factory), with the elution separation of 800ml pure water solution after sample introduction is complete, collected since efflux has momordica glycoside V > 1%.It collects
Efflux is concentrated under reduced pressure to 15 Baume degrees, is spray-dried, obtains snow-white momordica glycoside V.
Embodiment 2
Mature, fresh Siraitia grosvenorii 1kg is taken, is crushed, 4kg pure water is added to be beaten with beater.PH is adjusted with 4% citric acid to arrive
4.5, hemicellulase 0.5ml, 1,4 beta-glucanase 0.5ml, pectase 0.5ml totally three kinds of enzymes are first added, it is small in 42 DEG C of enzymatic hydrolysis 3
When.It is further continued for that pectin lyase 0.5ml is added by 200 mesh filter clothes, is digested 2 hours at a temperature of 48 DEG C, obtain enzymolysis liquid, enzyme
Solution liquid is heated to 83 DEG C, keeps progress enzyme deactivation in 8 minutes, lets cool to 27 DEG C, and high-activity yeast powder 1g is added and (is purchased from Angel Yeast
(Chongzuo) Co., Ltd), at ferment under aerobic conditions 4 hours.Fermentation liquid is crossed into organic film, first crosses the organic of 5000 molecular weight
Film, into film pressure 0.48MPa, after the organic film of 400 molecular weight, into film pressure 1.9MPa, must decolourize film liquid；It is straight to cross film liquid
The compound zwitterion resin column that resin loading is 200g is tapped into, wherein model D301R (weak base anion resins) and D152
(low-acid cationic resin) mass ratio is 2:1.2 (anions and canons resin is purchased from Tianjin Nankai resin processing plant), is used after sample introduction is complete
The elution separation of 800ml pure water solution, is collected since efflux has momordica glycoside V > 1%.Efflux is collected, reduced pressure is arrived
16 Baume degrees, spray drying, obtain snow-white momordica glycoside V.
Embodiment 3
Mature, fresh Siraitia grosvenorii 1kg is taken, is crushed, 5kg pure water is added to be beaten with beater.PH is adjusted with 5% citric acid to arrive
4.8, cellulase 0.6ml, hemicellulase 0.6ml, 1,4 beta-glucanase 0.6ml, pectase 0.6ml, cellulose water is first added
Enzyme 0.6ml totally five kinds of enzymes are solved, addition total amount is 3ml, is digested 5 hours at 45 DEG C.It is further continued for that papain is added by 200 mesh filter clothes
1ml, acid protease 1ml, amylase 1ml totally three kinds of enzymes digest 3 hours at 50 °C, obtain enzymolysis liquid, enzymolysis liquid adds
Heat keeps progress enzyme deactivation in 10 minutes, lets cool to 30 DEG C to 85 DEG C, and high-activity yeast powder 5g is added and (is purchased from Angel Yeast (Chongzuo)
Co., Ltd), at ferment under aerobic conditions 5 hours.Fermentation liquid is crossed into organic film, the organic film of 5000 molecular weight is first crossed, into film
Pressure 0.5MPa, after the organic film of 400 molecular weight, into film pressure 2.0MPa, must decolourize film liquid；Film liquid is crossed directly into resin
Loading is the compound zwitterion resin column of 200g, wherein model D301R (weak base anion resins) and D152 (weak acid sun from
Subtree rouge) mass ratio be 3:1.5 (anions and canons resin is purchased from Tianjin Nankai resin processing plant), sample introduction it is complete after use 800ml pure water
Solution elution separation, is collected since efflux has momordica glycoside V > 1%.Efflux is collected, is concentrated under reduced pressure to 18 Baume degrees,
Spray drying, obtains snow-white momordica glycoside V.
Comparative example 1
Present case preparation method is to cancel enzymolysis liquid on the basis of 1 preparation method of embodiment to be heated to 80 DEG C, is kept for 5 minutes
The step of carrying out enzyme deactivation, i.e., will be collected into enzymolysis liquid and directly carry out yeast fermentation, other techniques and parameter remain unchanged.
Comparative example 2
Present case preparation method is to cancel enzymolysis liquid on the basis of 2 preparation method of embodiment to be heated to 83 DEG C, is kept for 8 minutes
The step of carrying out enzyme deactivation, i.e., will be collected into enzymolysis liquid and directly carry out yeast fermentation, other techniques and parameter remain unchanged.
Comparative example 3
Present case preparation method is to cancel enzymolysis liquid on the basis of 3 preparation method of embodiment to be heated to 85 DEG C, is kept for 10 points
Clock carries out the step of enzyme deactivation, i.e., will be collected into enzymolysis liquid and directly carry out yeast fermentation, other techniques and parameter remain unchanged.
Comparative example 4
It with loading is 200g model D301R resin column that present case preparation method, which is on the basis of 1 preparation method of embodiment,
(weak base anion resins) are chromatographed instead of compound zwitterion resin column, other techniques and parameter remain unchanged.
Comparative example 5
Present case preparation method be on the basis of 1 preparation method of embodiment with loading be 200g model D152 (weak acid sun
Ion exchange resin) it is chromatographed instead of compound zwitterion resin column, other techniques and parameter remain unchanged.
Momordica glycoside V content obtained by Examples 1 to 3 is high, appearance is snow-white, good taste, no bitter taste, dissolvent residual
It is few, non agricultural chemical residuum, product with stable quality, after comparative example 1~5 changes corresponding technological parameter, Examples 1 to 3 and
1~5 detection method of comparative example is the same, and embodiment and comparative example products obtained therefrom comparative situation are shown in Table 1.
1 product content of table and character
Claims (10)
1. a kind of method for separating momordica glycoside V, which is characterized in that the method is to add Siraitia grosvenorii water to be beaten later successively
Plant enzyme enzymatic hydrolysis, biological enzyme enzymatic hydrolysis are carried out, enzymolysis liquid is collected, carries out yeast fermentation, compound cation and anion exchange tree after enzyme deactivation
Rouge purifying is concentrated and dried obtained momordica glycoside V；
The plant enzyme is at least one in cellulase, hemicellulase, 1,4 beta-glucanase, pectase or cellulolytic enzyme
Kind；
The biological enzyme is at least one in pectin lyase, papain, acid protease, amylase or paragalactan enzyme
Kind.
2. the method for separation momordica glycoside V according to claim 1, which is characterized in that in terms of kg/mL, the arhat
Fruit and plant enzyme mass volume ratio are 1: 0.5~3.
3. the method for separation momordica glycoside V according to claim 2, which is characterized in that plant enzyme enzymatic hydrolysis be
It is digested 1~5 hour under the conditions of PH is 4.0~4.8, temperature is 40~45 DEG C.
4. the method for separation momordica glycoside V according to claim 1, which is characterized in that in terms of kg/mL, the arhat
Fruit and biological enzyme mass volume ratio are 1: 0.5~15.
5. the method for separation momordica glycoside V according to claim 4, which is characterized in that the biological enzyme hydrolysis temperature
It is 45~50 DEG C, enzymolysis time is 1~3 hour.
6. it is according to claim 1 separation momordica glycoside V method, which is characterized in that the enzyme-removal temperature be 80~
85 DEG C, the enzyme deactivation time is 5~10 minutes.
7. the method for separation momordica glycoside V according to claim 1, which is characterized in that in terms of kg/g, the Siraitia grosvenorii
It is 1: 0.5~5 with yeast mass ratio；The yeast fermentation temperature is 25~30 DEG C, and fermentation time is 3~5 hours.
8. the method for separation momordica glycoside V according to claim 1, which is characterized in that the compound zwitterion is handed over
Changing purifying resin step further includes before organic membrane filter step, and the organic membrane filter step includes filtering and second for the first time
Secondary filtering, using the organic film of 5000 molecular weight of retention, filter pressure is 0.45~0.5MPa for the first time filtering；Described
For secondary filter using the organic film of 400 molecular weight of retention, filter pressure is 1.8~2.0MPa.
9. the method for separation momordica glycoside V according to claim 1, which is characterized in that the compound zwitterion is handed over
Changing resin anion (R.A.) and resin cation mass ratio in resin is 1~3:1~1.5；The compound anion-cation exchange resin is pure
Changing eluent used is water.
10. the method for separation momordica glycoside V according to claim 1, which is characterized in that be concentrated in the concentration step
Liquid concentration is 15~18 Baume degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811397385.4A CN109651480A (en) | 2018-11-22 | 2018-11-22 | A method of separation momordica glycoside V |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811397385.4A CN109651480A (en) | 2018-11-22 | 2018-11-22 | A method of separation momordica glycoside V |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109651480A true CN109651480A (en) | 2019-04-19 |
Family
ID=66111490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811397385.4A Pending CN109651480A (en) | 2018-11-22 | 2018-11-22 | A method of separation momordica glycoside V |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109651480A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110357936A (en) * | 2019-07-16 | 2019-10-22 | 湖南华诚生物资源股份有限公司 | The method of semi-synthetic momordica glycoside V |
| CN110468015A (en) * | 2019-08-30 | 2019-11-19 | 桂林莱茵生物科技股份有限公司 | A kind of flavor imitation health liquor and preparation method thereof |
| CN110467982A (en) * | 2019-08-30 | 2019-11-19 | 桂林莱茵生物科技股份有限公司 | A kind of flavor prepares beer and preparation method thereof |
| CN110679592A (en) * | 2019-09-30 | 2020-01-14 | 中国有色桂林矿产地质研究院有限公司 | Slow-release degradable pesticide granules based on high-performance adsorption material and preparation method thereof |
| CN111543530A (en) * | 2020-06-22 | 2020-08-18 | 湖南绿果甜品有限公司 | Preparation method of low-calorie fructus momordicae sweet tea light drink |
| CN112825952A (en) * | 2020-12-31 | 2021-05-25 | 桂林实力科技有限公司 | Low-glycemic-index fructus momordicae fibrous candy and preparation method thereof |
| WO2022264600A1 (en) * | 2021-06-14 | 2022-12-22 | Ｆｏｎｔｅｃ Ｒ＆Ｄ株式会社 | Monk fruit extract and method for producing same |
| CN115715891A (en) * | 2022-11-11 | 2023-02-28 | 湖南绿蔓生物科技股份有限公司 | Continuous method for separating multiple effective components from fructus Siraitiae Grosvenorii |
| CN117016780A (en) * | 2023-07-05 | 2023-11-10 | 珠海市华馨生物科技有限公司 | Preparation method of momordica grosvenori powder |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1907091A (en) * | 2006-08-11 | 2007-02-07 | 周英 | Process for removing bitter taste of momordica grosvenori extract |
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| WO2018157335A1 (en) * | 2017-03-01 | 2018-09-07 | 桂林莱茵生物科技股份有限公司 | Method for increasing content of mogroside v in siraitia grosvenorii suspended cells |
| CN108743647A (en) * | 2018-06-27 | 2018-11-06 | 广西驰胜农业科技有限公司 | A kind of preparation method of Fructus Monordicae extract |
-
2018
- 2018-11-22 CN CN201811397385.4A patent/CN109651480A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1907091A (en) * | 2006-08-11 | 2007-02-07 | 周英 | Process for removing bitter taste of momordica grosvenori extract |
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| WO2018157335A1 (en) * | 2017-03-01 | 2018-09-07 | 桂林莱茵生物科技股份有限公司 | Method for increasing content of mogroside v in siraitia grosvenorii suspended cells |
| CN108743647A (en) * | 2018-06-27 | 2018-11-06 | 广西驰胜农业科技有限公司 | A kind of preparation method of Fructus Monordicae extract |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110357936A (en) * | 2019-07-16 | 2019-10-22 | 湖南华诚生物资源股份有限公司 | The method of semi-synthetic momordica glycoside V |
| CN110468015A (en) * | 2019-08-30 | 2019-11-19 | 桂林莱茵生物科技股份有限公司 | A kind of flavor imitation health liquor and preparation method thereof |
| CN110467982A (en) * | 2019-08-30 | 2019-11-19 | 桂林莱茵生物科技股份有限公司 | A kind of flavor prepares beer and preparation method thereof |
| CN110679592A (en) * | 2019-09-30 | 2020-01-14 | 中国有色桂林矿产地质研究院有限公司 | Slow-release degradable pesticide granules based on high-performance adsorption material and preparation method thereof |
| CN111543530A (en) * | 2020-06-22 | 2020-08-18 | 湖南绿果甜品有限公司 | Preparation method of low-calorie fructus momordicae sweet tea light drink |
| CN111543530B (en) * | 2020-06-22 | 2023-06-02 | 湖南华诚生物资源股份有限公司 | Preparation method of low-calorie momordica grosvenori sweet tea light drink |
| CN112825952A (en) * | 2020-12-31 | 2021-05-25 | 桂林实力科技有限公司 | Low-glycemic-index fructus momordicae fibrous candy and preparation method thereof |
| WO2022264600A1 (en) * | 2021-06-14 | 2022-12-22 | Ｆｏｎｔｅｃ Ｒ＆Ｄ株式会社 | Monk fruit extract and method for producing same |
| CN115715891A (en) * | 2022-11-11 | 2023-02-28 | 湖南绿蔓生物科技股份有限公司 | Continuous method for separating multiple effective components from fructus Siraitiae Grosvenorii |
| CN117016780A (en) * | 2023-07-05 | 2023-11-10 | 珠海市华馨生物科技有限公司 | Preparation method of momordica grosvenori powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109651480A (en) | A method of separation momordica glycoside V | |
| CN109593034B (en) | Method for preparing shikimic acid from ginkgo leaf extraction waste liquid | |
| CN101664138B (en) | Fructus momordicae instant powder and preparation method thereof | |
| CN101214281B (en) | Method for producing cistanche salsa extract | |
| CN105294790A (en) | Method for extracting high-purity steviol glycosides from stevia rebaudiana | |
| CN107858393A (en) | A kind of method that polypeptide is extracted from walnut dregs | |
| CN107226871B (en) | preparation method of highland barley β -glucan | |
| CN104152491B (en) | Fermented Radix Astragali preparation method, and method for extracting total saponins of fermented Radix Astragali | |
| CN103266154A (en) | Biological transformation method for preparing high-activity theasaponin | |
| CN101747196A (en) | Method for preparing chlorogenic acid by using Jerusalem artichoke | |
| CN111588043B (en) | Preparation method of dietary fiber based on Siraitia grosvenorii waste | |
| CN108997443A (en) | A kind of method that microbial fermentation extracts saponin(e in plant | |
| CN111296708A (en) | Method for producing fructus momordicae extract by adopting immobilized enzyme technology | |
| CN104357332B (en) | Aspergillus niger JH-2 and application to biotransformation and synthesis of asiatic acid | |
| CN105053952B (en) | A kind of processing technology of the dried orange peel extracts of no bitter taste | |
| CN102070479A (en) | Method for breaking cell wall of chilli by using cellulase | |
| CN114177218B (en) | Mulberry leaf extract rich in 1-deoxynojirimycin and preparation method thereof | |
| CN101194934B (en) | Method for abstracting active compound rich in echinacoside and feltwort glycoside | |
| CN1927857A (en) | Extraction and purification process of buckwheat sprout total flavone | |
| CN113402626A (en) | Nymphaea hybrid polysaccharide extract and preparation method and application thereof | |
| CN103275151B (en) | A kind of process for purification of Matachrom | |
| CN104774827A (en) | Method for preparing alginate lyase from abalone internal organs | |
| CN109485559A (en) | A method of extracting shikimic acid from illiciumverum | |
| CN115029385B (en) | Method for extracting tea saponin from tea seed cake by microbial fermentation | |
| CN104357527B (en) | A kind of method that microbe fermentation method extracts Tea Saponin from leached tea oil slag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |