CN108743647A - A kind of preparation method of Fructus Monordicae extract - Google Patents
A kind of preparation method of Fructus Monordicae extract Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods of Fructus Monordicae extract, belong to Siraitia grosvenorii processing technique field.The present invention includes the following steps:(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, crushes, obtains mangosteen powder;(2) lemon, pyrus nivalis, white sugar, saccharomycete, water are mixed, sealing and fermenting prepares extracting solution;(3) mangosteen powder is added in extracting solution, produces Siraitia grosvenorii crude extract;(4) ethyl alcohol is added in Siraitia grosvenorii crude extract, Siraitia grosvenorii crude extract is obtained using ultrasonic wave assisted extraction;(5) Siraitia grosvenorii crude extract is taken to be adsorbed;It is eluted using 30% ethyl alcohol;(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.The preparation method of the present invention is simple, and the Momordia grosvenori aglycone content of Fructus Monordicae extract obtained is high, and does not have taste of traditional Chinese medicine and rear bitter taste, in good taste.
Description
Technical field
The present invention relates to Siraitia grosvenorii processing technique fields, and in particular to a kind of preparation method of Fructus Monordicae extract.
Background technology
Siraitia grosvenorii, is the fruit of cucurbitaceous plant Siraitia grosvenorii, and autumn fruit harvests when becoming bottle green by peak green.Often it is born in
In hillside hayashishita and river bank wetland, bushes, it is distributed in the ground such as Jiangxi, Hunan, Guangdong, Guangxi, Guizhou.Traditional Chinese medicine and pharmacy thinks,
The effect of Siraitia grosvenorii is sweet, cool in nature, and there are clearing heat and moistening lung, relieving sore-throat to open sound, laxation defaecation, is used for lung-heat type cough, pharyngalgia aphonia, intestinal dryness
Constipation.The edible crowd of Siraitia grosvenorii is very extensive, and whether pregnant woman, child, teenager, wage-earners, the middle-aged and the old have edible
Distribution.
Siraitia grosvenorii is the medicine-food two-purpose rare traditional Chinese medicine that the Ministry of Public Health announces in the first batch, and contained Momordica-Glycosides is than sucrose sweet tea
300 times, do not generate heat, be beverage, confectionery industry rare raw material, be the best substitute of sucrose.Often drink " Luohan " fruit tea, can
Prevent that a variety of diseases, modern medicine prove that Siraitia grosvenorii has significant curative effect to diseases such as bronchitis, hypertension, moreover it is possible to play prevention
The effect of coronary heart disease, vascular sclerosis, obesity.Siraitia grosvenorii nutritive value is high, has clearing away summerheat, preventing phlegm from forming and stopping coughing, cool blood to relax bone, clear
It lung ease constipation and promotes the production of body fluid to quench thirst and other effects;It can treating acute and chronic tracheitis, sphagitis, bronchial asthma, pertussis, stomach energy, constipation, urgency
Diseases, the diabetics such as property tonsillitis also preferably take.
Currently, the preparation method of Fructus Monordicae extract mainly have it is following two, first, water carries plus resin partition method, second is that alcohol
Carry membrane separation process, Fructus Monordicae extract obtained by the above method is there are strong rear bitter taste, the problems such as taste of traditional Chinese medicine is denseer, shadow
The mouthfeel of Fructus Monordicae extract is rung.
Invention content
The purpose of the present invention is to solve the above problems of the existing technology, provide a kind of system of Fructus Monordicae extract
Preparation Method, preparation method of the invention is simple, and the Momordia grosvenori aglycone content of Fructus Monordicae extract obtained is high, and does not have taste of traditional Chinese medicine
And rear bitter taste, it is in good taste.
In order to achieve the above objectives, the technical solution adopted by the present invention is as follows:
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, adjusts stir-frying temperature
Degree is 60-70 DEG C, and the percentage of water loss to stir-fry to Siraitia grosvenorii reaches 70-80%, takes out Siraitia grosvenorii, crushes, obtain mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 3-5 days, fermented
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 2-3 days, filter to take filtrate, obtain extracting solution;
(3) mangosteen powder is added in extracting solution, is uniformly mixed, stood 10-15h, obtain Siraitia grosvenorii crude extract;
(4) ethyl alcohol that volume fraction is 70-80% is added in Siraitia grosvenorii crude extract, uses ultrasonic wave auxiliary after mixing
It is 40-48 DEG C, extraction time 15-20h, working frequency 25-40KHz to help extraction, Extracting temperature, and filtering takes filtrate to obtain
Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, selects macroporous absorbent resin D-101 to be adsorbed, sample solution concentration 6.2-
7.0mg/mL, adsorption flow rate 0.5-1.0BV/h stop loading when reaching leak point;Use volume fraction for 30% ethanol water
Solution is that eluant, eluent is eluted, and stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Above-mentioned technical proposal, preferably, in step (1), the addition of the clam meal is the 30- of Siraitia grosvenorii weight
40%.
Above-mentioned technical proposal, preferably, in step (2), the raw material of the extracting solution is counted as follows in parts by weight:Lemon
10-20 parts of lemon, 30-50 parts of pyrus nivalis, 5-10 parts of white sugar, 0.1-0.3 parts of saccharomycete, 0.05-0.1 parts of acetic acid bacteria, water 400-500
Part.
Above-mentioned technical proposal, preferably, in step (3), the mass ratio of the mangosteen powder and extracting solution is 1:3-4.
Above-mentioned technical proposal, preferably, in step (4), the addition of the ethyl alcohol is Siraitia grosvenorii crude extract weight
10-20%.
Above-mentioned technical proposal, preferably, in step (4), the Extracting temperature of the ultrasonic wave assisted extraction is 45 DEG C, is carried
It is 18h, working frequency 28KHz to take the time.
Above-mentioned technical proposal, preferably, in step (5), the sample solution concentration is 6.6mg/mL, and adsorption flow rate is
0.7BV/h。
Advantageous effect:
1, fresh Siraitia grosvenorii and clam meal are first carried out stir-frying drying by the present invention at a certain temperature, can improve Siraitia grosvenorii
The drug effect of extract, and more utilize the use of cooperation later stage extracting solution.It is same in the Fructus Monordicae extract that the present invention is prepared
Momordia grosvenori aglycone IV, the momordica grosvenori glycoside V of Shi Hanyou high-contents effectively improve human detoxification energy to the adjusting improvement result of intestinal tube
Power enhances human immunity.In addition, momordica grosvenori glycoside V includes abundant vitamin C, there is anti-aging, anticancer, beneficial liver spleen and support
The effect of skin beauty.
2, the present invention is fermented to obtain extracting solution by lemon, pyrus nivalis, white sugar, saccharomycete, water and acetic acid bacteria, then with
70-80% ethyl alcohol coordinates, and auxiliary ultrasonic extracts, and can effectively extract the active principle of Siraitia grosvenorii, improves Siraitia grosvenorii
The health-care effect of extract;It is adsorbed with macroporous absorbent resin D-101, the elution of 30% ethanol water being capable of purification
Object removes undesired impurities.By the mutual cooperation of each step, Fructus Monordicae extract produced by the present invention is in good taste, without Chinese medicine
Taste and rear bitter taste.
Specific implementation mode
With reference to specific embodiment, the invention will be further described.
Embodiment 1
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, the addition of clam meal
Amount is the 35% of Siraitia grosvenorii weight;It is 65 DEG C to adjust stir-frying temperature, and stir-frying to the percentage of water loss of Siraitia grosvenorii takes out arhat up to 75%
Fruit crushes, obtains mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 4 days, in fermentate
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 2.5 days, filter to take filtrate, obtain extracting solution;
The raw material of the extracting solution is counted as follows in parts by weight:14 parts of lemon, 40 parts of pyrus nivalis, 7 parts of white sugar, saccharomycete 0.2
Part, 0.08 part of acetic acid bacteria, 450 parts of water.
(3) mangosteen powder is added in extracting solution, the mass ratio of mangosteen powder and extracting solution is 1:3.5, it is uniformly mixed, it is quiet
14h is set, Siraitia grosvenorii crude extract is obtained;
(4) ethyl alcohol that volume fraction is 75% is added in Siraitia grosvenorii crude extract, the addition of 75% ethyl alcohol is sieve
The 10-20% of Chinese fruit crude extract weight, it is 45 DEG C to use ultrasonic wave assisted extraction, Extracting temperature after mixing, extraction time
For 18h, working frequency 28KHz, filtering takes filtrate to obtain Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, selects macroporous absorbent resin D-101 to be adsorbed, the sample solution of Siraitia grosvenorii crude extract
A concentration of 6.6mg/mL, adsorption flow rate 0.7BV/h stop loading when reaching leak point;Use volume fraction for 30% ethyl alcohol
Aqueous solution is that eluant, eluent is eluted, and stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Embodiment 2
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, the addition of clam meal
Amount is the 32% of Siraitia grosvenorii weight;It is 62 DEG C to adjust stir-frying temperature, and stir-frying to the percentage of water loss of Siraitia grosvenorii takes out arhat up to 73%
Fruit crushes, obtains mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 3 days, in fermentate
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 3 days, filter to take filtrate, obtain extracting solution;
The raw material of the extracting solution is counted as follows in parts by weight:12 parts of lemon, 35 parts of pyrus nivalis, 7 parts of white sugar, saccharomycete 0.1
Part, 0.06 part of acetic acid bacteria, 420 parts of water.
(3) mangosteen powder is added in extracting solution, the mass ratio of mangosteen powder and extracting solution is 1:3, it is uniformly mixed, stands
11h obtains Siraitia grosvenorii crude extract;
(4) ethyl alcohol that volume fraction is 75% is added in Siraitia grosvenorii crude extract, the addition of the ethyl alcohol is Siraitia grosvenorii
The 12% of crude extract weight, it is 43 DEG C, extraction time 16h to use ultrasonic wave assisted extraction, Extracting temperature after mixing, work
Working frequency is 25KHz, and filtering takes filtrate to obtain Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, macroporous absorbent resin D-101 is selected to be adsorbed, sample solution concentration 6.4mg/mL,
Adsorption flow rate is 0.8BV/h, stops loading when reaching leak point;Use volume fraction for 30% ethanol water be eluant, eluent into
Row elution, stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Embodiment 3
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, the addition of clam meal
Amount is the 38% of Siraitia grosvenorii weight;It is 68 DEG C to adjust stir-frying temperature, and stir-frying to the percentage of water loss of Siraitia grosvenorii takes out arhat up to 76%
Fruit crushes, obtains mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 5 days, in fermentate
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 2 days, filter to take filtrate, obtain extracting solution;
The raw material of the extracting solution is counted as follows in parts by weight:18 parts of lemon, 45 parts of pyrus nivalis, 8 parts of white sugar, saccharomycete 0.3
Part, 0.09 part of acetic acid bacteria, 480 parts of water.
(3) mangosteen powder is added in extracting solution, the mass ratio of mangosteen powder and extracting solution is 1:3.8, it is uniformly mixed, it is quiet
14h is set, Siraitia grosvenorii crude extract is obtained;
(4) ethyl alcohol that volume fraction is 78% is added in Siraitia grosvenorii crude extract, the addition of the ethyl alcohol is Siraitia grosvenorii
The 17% of crude extract weight, it is 46 DEG C, extraction time 18h to use ultrasonic wave assisted extraction, Extracting temperature after mixing, work
Working frequency is 40KHz, and filtering takes filtrate to obtain Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, macroporous absorbent resin D-101 is selected to be adsorbed, sample solution concentration 6.8mg/mL,
Adsorption flow rate is 0.6BV/h, stops loading when reaching leak point;Use volume fraction for 30% ethanol water be eluant, eluent into
Row elution, stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Embodiment 4
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, the addition of clam meal
Amount is the 30% of Siraitia grosvenorii weight;It is 60 DEG C to adjust stir-frying temperature, and stir-frying to the percentage of water loss of Siraitia grosvenorii takes out arhat up to 70%
Fruit crushes, obtains mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 3 days, in fermentate
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 2 days, filter to take filtrate, obtain extracting solution;
The raw material of the extracting solution is counted as follows in parts by weight:10 parts of lemon, 30 parts of pyrus nivalis, 5 parts of white sugar, saccharomycete 0.1
Part, 0.05 part of acetic acid bacteria, 400 parts of water.
(3) mangosteen powder is added in extracting solution, the mass ratio of mangosteen powder and extracting solution is 1:3, it is uniformly mixed, stands
10h obtains Siraitia grosvenorii crude extract;
(4) ethyl alcohol that volume fraction is 70% is added in Siraitia grosvenorii crude extract, the addition of the ethyl alcohol is Siraitia grosvenorii
The 20% of crude extract weight, it is 40 DEG C, extraction time 20h to use ultrasonic wave assisted extraction, Extracting temperature after mixing, work
Working frequency is 25KHz, and filtering takes filtrate to obtain Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, macroporous absorbent resin D-101 is selected to be adsorbed, sample solution concentration 6.2mg/mL,
Adsorption flow rate is 1.0BV/h, stops loading when reaching leak point;Use volume fraction for 30% ethanol water be eluant, eluent into
Row elution, stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Embodiment 5
A kind of preparation method of Fructus Monordicae extract, includes the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, the addition of clam meal
Amount is the 40% of Siraitia grosvenorii weight;It is 70 DEG C to adjust stir-frying temperature, and stir-frying to the percentage of water loss of Siraitia grosvenorii takes out arhat up to 80%
Fruit crushes, obtains mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 5 days, in fermentate
Acetic acid bacteria is added in material, is uniformly mixed and continues fermentation 3 days, filter to take filtrate, obtain extracting solution;
The raw material of the extracting solution is counted as follows in parts by weight:20 parts of lemon, 50 parts of pyrus nivalis, 10 parts of white sugar, saccharomycete
0.3 part, 0.1 part of acetic acid bacteria, 500 parts of water.
(3) mangosteen powder is added in extracting solution, the mass ratio of mangosteen powder and extracting solution is 1:4, it is uniformly mixed, stands
15h obtains Siraitia grosvenorii crude extract;
(4) ethyl alcohol that volume fraction is 80% is added in Siraitia grosvenorii crude extract, the addition of the ethyl alcohol is Siraitia grosvenorii
The 10% of crude extract weight, it is 48 DEG C, extraction time 15h to use ultrasonic wave assisted extraction, Extracting temperature after mixing, work
Working frequency is 25KHz, and filtering takes filtrate to obtain Siraitia grosvenorii crude extract;
(5) Siraitia grosvenorii crude extract is taken, macroporous absorbent resin D-101 is selected to be adsorbed, sample solution concentration 7.0mg/mL,
Adsorption flow rate is 0.5BV/h, stops loading when reaching leak point;Use volume fraction for 30% ethanol water be eluant, eluent into
Row elution, stops when efflux reaches leak point, collects eluent;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
Comparative example 1
A kind of preparation method of Fructus Monordicae extract, it is substantially the same manner as Example 1, the difference is that:Mangosteen powder is
Directly carried out obtained by powder processed using dry Siraitia grosvenorii.
Comparative example 2
A kind of preparation method of Fructus Monordicae extract, it is substantially the same manner as Example 1, the difference is that:Save step (2)
With step (3), directly mangosteen powder is added in the ethyl alcohol that volume fraction is 75% and is extracted.
Comparative example 3
A kind of preparation method of Fructus Monordicae extract, takes dried fructus momordicae, is carried using traditional water plus resin partition method carries out
Extraction.
Comparative example 4
A kind of preparation method of Fructus Monordicae extract, takes dried fructus momordicae, is extracted using traditional alcohol extracting membrane separation process.
Embodiment 1-3, comparative example the 1-4 Fructus Monordicae extract being prepared are detected, testing result is shown in Table 1.
Table 1
Embodiment 1-3 is prepared it can be seen from the data of table 1 Fructus Monordicae extract while sieve with high-content
The Chinese fruit glycosides V and Momordia grosvenori aglycone IV, and it is in good taste.And the momordica grosvenori glycoside V and Momordia grosvenori aglycone IV contents of comparative example 1 and comparative example 2
Declined, mouthfeel evaluation is opposite to be deteriorated, and shows to prepare mangosteen powder after the present invention is stir-fried using new fresh fructus momordicae, right
Extraction effect has certain influence, and extracting solution prepared by the present invention has large effect to the extraction effect of Fructus Monordicae extract.
By the comparison of comparative example 3, comparative example 4 and embodiment 1 it is found that the preparation method of the present invention compares traditional extracting method, no matter
It is the content or mouthfeel aspect of Momordia grosvenori aglycone, has greatly improved, Fructus Monordicae extract produced by the present invention is obviously than passing
The Fructus Monordicae extract better quality that system method obtains, and the method for the present invention is comparatively fairly simple, easy to operate, energy
Enough reduce manufacturing cost.
Although the present invention is disclosed as above with preferred embodiment, so it is not intended to limiting the invention, any this field skill
Art personnel, without departing from the spirit and scope of the present invention, when a little modification and perfect, therefore the protection model of the present invention can be made
It encloses to work as and is subject to what claims were defined.
Claims (7)
1. a kind of preparation method of Fructus Monordicae extract, which is characterized in that include the following steps:
(1) fresh Siraitia grosvenorii is picked, is put into automatic material frying machine and stir-fries, while clam meal is added, adjusting stir-frying temperature is
60-70 DEG C, the percentage of water loss to stir-fry to Siraitia grosvenorii reaches 70-80%, takes out Siraitia grosvenorii, crushes, obtain mangosteen powder;
(2) lemon, pyrus nivalis are sliced, then are uniformly mixed with white sugar, saccharomycete, water, after being sealed by fermentation 3-5 days, in fermentation materials
Middle addition acetic acid bacteria is uniformly mixed and continues fermentation 2-3 days, filters to take filtrate, obtain extracting solution;
(3) mangosteen powder is added in extracting solution, is uniformly mixed, stood 10-15h, obtain Siraitia grosvenorii crude extract;
(4) ethyl alcohol that volume fraction is 70-80% is added in Siraitia grosvenorii crude extract, ultrasonic wave auxiliary is used to carry after mixing
It takes, Extracting temperature is 40-48 DEG C, extraction time 15-20h, working frequency 25-40KHz, and filtering takes filtrate to obtain arhat
Fruit crude extract;
(5) Siraitia grosvenorii crude extract is taken, macroporous absorbent resin D-101 is selected to be adsorbed, sample solution concentration 6.2-7.0mg/mL,
Adsorption flow rate is 0.5-1.0BV/h, stops loading when reaching leak point;It for 30% ethanol water is elution to use volume fraction
Agent is eluted, and is stopped when efflux reaches leak point, and eluent is collected;
(6) eluent is subjected to vacuum distillation and is condensed into medicinal extract, then be spray-dried, you can obtain Fructus Monordicae extract.
2. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (1), the clam meal
Addition is the 30-40% of Siraitia grosvenorii weight.
3. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (2), the extracting solution
Raw material, in parts by weight count it is as follows:10-20 parts of lemon, 30-50 parts of pyrus nivalis, 5-10 parts of white sugar, 0.1-0.3 parts of saccharomycete, vinegar
Sour bacterium 0.05-0.1 parts, 400-500 parts of water.
4. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (3), the Siraitia grosvenorii
The mass ratio of powder and extracting solution is 1:3-4.
5. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (4), the ethyl alcohol
Addition is the 10-20% of Siraitia grosvenorii crude extract weight.
6. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (4), the ultrasonic wave
The Extracting temperature of assisted extraction is 45 DEG C, extraction time 18h, working frequency 28KHz.
7. the preparation method of Fructus Monordicae extract as described in claim 1, it is characterised in that:In step (5), the sample solution
A concentration of 6.6mg/mL, adsorption flow rate 0.7BV/h.
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CN111956567A (en) * | 2020-09-30 | 2020-11-20 | 邓婕 | Hair physiological regulator and preparation and application methods thereof |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109651480A (en) * | 2018-11-22 | 2019-04-19 | 桂林莱茵生物科技股份有限公司 | A method of separation momordica glycoside V |
CN111956567A (en) * | 2020-09-30 | 2020-11-20 | 邓婕 | Hair physiological regulator and preparation and application methods thereof |
CN113729104A (en) * | 2021-08-23 | 2021-12-03 | 仲恺农业工程学院 | Preparation method of momordica grosvenori sugaring liquid and low-sugar momordica grosvenori green plum preserved fruit |
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