CN110548075A - Preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid - Google Patents
Preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid Download PDFInfo
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- 241000218671 Ephedra Species 0.000 title claims abstract description 49
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 title claims abstract description 34
- 229910052602 gypsum Inorganic materials 0.000 title claims abstract description 34
- 239000010440 gypsum Substances 0.000 title claims abstract description 34
- 239000007788 liquid Substances 0.000 title claims abstract description 30
- 244000018633 Prunus armeniaca Species 0.000 title claims abstract description 27
- 235000009827 Prunus armeniaca Nutrition 0.000 title claims abstract description 27
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 title claims abstract description 22
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 title claims abstract description 22
- 229940010454 licorice Drugs 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 240000004670 Glycyrrhiza echinata Species 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 27
- 230000004151 fermentation Effects 0.000 claims abstract description 27
- 238000000605 extraction Methods 0.000 claims abstract description 23
- 241000202807 Glycyrrhiza Species 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 13
- 244000303040 Glycyrrhiza glabra Species 0.000 claims abstract description 12
- 235000011477 liquorice Nutrition 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 238000002791 soaking Methods 0.000 claims abstract description 9
- 239000008213 purified water Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 4
- 241001310537 Acetobacter orientalis Species 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 3
- KWGRBVOPPLSCSI-WPRPVWTQSA-N (-)-ephedrine Chemical compound CN[C@@H](C)[C@H](O)C1=CC=CC=C1 KWGRBVOPPLSCSI-WPRPVWTQSA-N 0.000 abstract description 16
- KWGRBVOPPLSCSI-UHFFFAOYSA-N d-ephedrine Natural products CNC(C)C(O)C1=CC=CC=C1 KWGRBVOPPLSCSI-UHFFFAOYSA-N 0.000 abstract description 9
- 229960002179 ephedrine Drugs 0.000 abstract description 8
- 244000144725 Amygdalus communis Species 0.000 abstract description 6
- 238000011049 filling Methods 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 230000000857 drug effect Effects 0.000 abstract description 2
- 239000000284 extract Substances 0.000 abstract 3
- 235000003893 Prunus dulcis var amara Nutrition 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 239000004743 Polypropylene Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 235000011437 Amygdalus communis Nutrition 0.000 description 5
- 235000020224 almond Nutrition 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 241001465251 Ephedra sinica Species 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- FMCGSUUBYTWNDP-ONGXEEELSA-N (1R,2S)-2-(dimethylamino)-1-phenyl-1-propanol Chemical compound CN(C)[C@@H](C)[C@H](O)C1=CC=CC=C1 FMCGSUUBYTWNDP-ONGXEEELSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 235000018142 Hedysarum alpinum var americanum Nutrition 0.000 description 1
- 240000006461 Hedysarum alpinum var. americanum Species 0.000 description 1
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 description 1
- FMCGSUUBYTWNDP-UHFFFAOYSA-N N-Methylephedrine Natural products CN(C)C(C)C(O)C1=CC=CC=C1 FMCGSUUBYTWNDP-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 239000001685 glycyrrhizic acid Substances 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 239000008570 maxingshigan Substances 0.000 description 1
- 229960002221 methylephedrine Drugs 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- KWGRBVOPPLSCSI-WCBMZHEXSA-N pseudoephedrine Chemical compound CN[C@@H](C)[C@@H](O)C1=CC=CC=C1 KWGRBVOPPLSCSI-WCBMZHEXSA-N 0.000 description 1
- 229960003908 pseudoephedrine Drugs 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/17—Gnetophyta, e.g. Ephedraceae (Mormon-tea family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Pulmonology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
the invention relates to a preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid, which comprises the following steps: (1) taking 500 parts by weight of ephedra 300-; (2) pulverizing the ephedra herb in the step (1), adding purified water for soaking, and inoculating a fermentation strain for fermentation after soaking completely; (3) carrying out strain fermentation on the ephedra in the step (2), then carrying out normal pressure decoction extraction to obtain an ephedra extract, and concentrating the ephedra extract for later use after filtering; (4) respectively carrying out normal pressure decoction extraction on the bitter apricot seeds, the gypsum and the liquorice in the step (1) to respectively obtain extracting solutions of the medicinal materials, and respectively concentrating the extracting solutions for later use after filtering; (5) mixing the concentrated solutions obtained in the steps (3) and (4), continuously concentrating, filling and sterilizing to obtain the ephedra, apricot kernel, gypsum and licorice oral liquid; the invention adopts a strain fermentation method to extract the ephedra, enhances the ephedrine content of the extracting solution, and separately extracts the bitter almond, the liquorice and the gypsum, thereby having the advantages of enhancing the drug effect and reducing the cost.
Description
Technical Field
The invention belongs to the technical field of preparation of veterinary traditional Chinese medicine oral liquid, and particularly relates to a preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid.
background
The ephedra, almond, gypsum and liquorice root oral liquid is a traditional Chinese medicine oral liquid for chicken, which is recorded in the second volume of national standard for local standard of veterinary drugs, has the main components of ephedra, almond, gypsum and liquorice, has the effects of clearing heat, ventilating and smoothing lung and relieving asthma, is mainly used for treating lung heat cough and asthma, and is clinically used for preventing and treating various respiratory diseases of ten chickens; the Chinese ephedra is one of the core medicinal materials of the medicine, wherein the main active effects of the Chinese ephedra are ephedrine, pseudoephedrine and methylephedrine, and the ephedrine monomers are unstable, so the ephedrine exists in the Chinese ephedra mainly in the form of salt and accounts for 80 to 85 percent of the total alkaloid, while the conventional domestic high-pressure extraction method is difficult to extract the effective components such as ephedrine and the like, thereby not only reducing the medicine effect, but also wasting resources and increasing the cost.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a preparation method of a Maxingshigan oral liquid to enhance the drug effect, reduce the resource waste and reduce the cost.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid comprises the following steps:
(1) Taking 500 parts by weight of ephedra 300-;
(2) taking the ephedra herb with the formula amount in the step (1), crushing the ephedra herb, adding purified water for soaking, inoculating a fermentation strain for fermentation after soaking thoroughly, wherein the inoculation amount is 8-12%, the fermentation time is 15h, and the fermentation temperature is 35-40 ℃;
(3) performing normal-pressure decoction extraction on the ephedra herb in the step (2) after strain fermentation, wherein the extraction temperature is 80-90 ℃, the material-liquid ratio is 1:8-1:12, repeating the extraction for 2 times, and the extraction time is 40min each time to obtain an ephedra herb extracting solution, filtering the ephedra herb extracting solution, and concentrating the ephedra herb to 500mL for later use;
(4) Taking the bitter apricot seeds, the gypsum and the liquorice in the formula amount in the step (1) to respectively carry out normal pressure decoction extraction, wherein the water adding amount is 8-10 times, the decoction temperature is 95-100 ℃, the extraction time is 45min, extracting solutions of the medicinal materials are respectively obtained, and after filtration, the extracting solutions are respectively concentrated to 500mL of 300-;
(5) and (4) mixing the concentrated solutions obtained in the steps (3) and (4), continuously concentrating to 1300mL of 1000-.
in the step (2), the fermentation strain is a fermentation strain with a preservation number of CCTCC NO: m2015455 Acetobacter orientalis.
in the step (2), the inoculation amount is 10%.
In the step (2), the fermentation temperature is 38 ℃.
In the step (3), the extraction temperature is 85 ℃.
In the step (3), the ratio of the material to the liquid is 1: 10.
In the step (4), the decoction temperature is 100 ℃.
in the step (4), the filter element used for filtering is a PP folded filter element, and the filtering aperture is 0.44 μm.
the invention has the technical effects and advantages that:
the preparation method has the advantages that the herba ephedrae is extracted by adopting a strain fermentation method, so that the precipitation of ephedrine serving as an effective component of the herba ephedrae is increased, the ephedrine content and the treatment effect of an extracting solution are enhanced, meanwhile, the bitter apricot seeds, the liquorice and the gypsum are independently extracted, the content reduction caused by mixed extraction is avoided due to different extraction processes of each medicinal material, and the treatment effect of the herba ephedrae and licorice oral liquid is greatly enhanced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The strain Acetobacter orientalis used in the following examples was developed by the company itself and obtained the national strain preservation certificate, the Acetobacter orientalis was deposited under the accession number T1-1 at 7.16.2015 in the China center for type culture Collection, the Wuhan university was assigned the accession number CCTCC NO: M2015455.
Example 1:
the preparation method of the ephedra, apricot kernel, gypsum and licorice oral liquid is characterized by comprising the following steps:
(1) Taking 300 g of ephedra, 300 g of bitter apricot kernel, 1500 g of gypsum and 300 g of liquorice for standby;
(2) Crushing the ephedra herb in the step (1), adding purified water for soaking, and then inoculating acetobacter orientalis, wherein the inoculation amount is 10%, the fermentation time is 15h, and the fermentation temperature is 38 ℃;
(3) Fermenting herba Ephedrae in step (2) with Acetobacter orientalis, decocting and extracting under normal pressure, adding 10 times of water, extracting at 85 deg.C for 2 times (each time for 40 min) to obtain herba Ephedrae extractive solution, filtering, concentrating, and concentrating to 300 mL;
(4) Adding 8 times of water into the bitter apricot kernels, the gypsum and the liquorice in the step (1) respectively, decocting and extracting at the normal pressure at the extraction temperature of 100 ℃ for 45min to obtain three medicinal materials respectively to obtain extracting solutions, filtering the extracting solutions by a PP (polypropylene) folding filter element, and concentrating the extracting solutions to 300mL, 1500mL and 300mL respectively;
(5) And (4) mixing the extracting solutions obtained in the steps (3) and (4), continuing to concentrate after mixing, concentrating to 1000mL, filling, and sterilizing to obtain the ephedra, almond, gypsum and licorice oral liquid.
Example 2:
The preparation method of the ephedra, apricot kernel, gypsum and licorice oral liquid is characterized by comprising the following steps:
(1) Taking 500 g of ephedra, 300 g of bitter apricot kernel, 1700 g of gypsum and 400 g of liquorice for standby;
(2) crushing the ephedra herb in the step (1), adding purified water for soaking, and then inoculating acetobacter orientalis, wherein the inoculation amount is 10%, the fermentation time is 15h, and the fermentation temperature is 38 ℃;
(3) fermenting herba Ephedrae in step (2) with Acetobacter orientalis, decocting and extracting under normal pressure, adding 10 times of water, extracting at 85 deg.C for 2 times (each time for 40 min) to obtain herba Ephedrae extractive solution, filtering, concentrating, and concentrating to 500 mL;
(4) Adding 8 times of water into the bitter apricot kernels, the gypsum and the liquorice in the step (1) respectively, decocting and extracting at the normal pressure at the extraction temperature of 100 ℃ for 45min to obtain extracting solutions of the three medicinal materials respectively, filtering the extracting solutions by a PP (polypropylene) folding filter element, and concentrating the extracting solutions to 300mL, 1700mL and 400mL respectively;
(5) and (4) mixing the extracting solutions obtained in the steps (3) and (4), continuing to concentrate after mixing, concentrating to 1150mL, filling, and sterilizing to obtain the ephedra, almond, gypsum and licorice oral liquid.
Example 3:
the preparation method of the ephedra, apricot kernel, gypsum and licorice oral liquid is characterized by comprising the following steps:
(1) Taking 400 g of ephedra, 400 g of bitter apricot kernel, 1800 g of gypsum and 500 g of liquorice for standby;
(2) Crushing the ephedra herb in the step (1), adding purified water for soaking, and then inoculating acetobacter orientalis, wherein the inoculation amount is 10%, the fermentation time is 15h, and the fermentation temperature is 38 ℃;
(3) fermenting herba Ephedrae in step (2) with Acetobacter orientalis, decocting and extracting under normal pressure, adding 10 times of water, extracting at 85 deg.C for 2 times (each time for 40 min) to obtain herba Ephedrae extractive solution, filtering, concentrating, and concentrating to 400 mL;
(4) adding 8 times of water into the bitter apricot kernels, the gypsum and the liquorice in the step (1) respectively, decocting and extracting at the normal pressure, wherein the extraction temperature is 100 ℃, the extraction time is 45min, obtaining three medicinal materials respectively to obtain extracting solutions, filtering by a PP (polypropylene) folding filter element, and concentrating to 300mL, 1400mL and 500mL respectively;
(5) And (4) mixing the extracting solutions obtained in the steps (3) and (4), continuously concentrating after mixing, concentrating to 1250mL, filling, and sterilizing to obtain the ephedra, almond, gypsum and licorice oral liquid.
product identification: the product was identified according to the method of identification disclosed in the Ministry of agriculture 1760.
and (3) identification result:
the test results of pharmacopeia standards, conventional preparation processes and the solutions prepared in examples 1 to 3 of the present invention are compared as shown in table 1:
table 1: comparison of pharmacopeia standards, general preparation Process and test results of the solutions prepared in examples 1 to 3 of the present invention
As can be seen from the data in table 1, the respective results of the ephedra, gypsum and licorice oral liquid prepared in embodiments 1 to 3 of the present invention all meet the specifications, and compared with the conventional preparation process, the contents of ephedrine and glycyrrhizic acid of the present invention are both significantly increased.
Claims (8)
1. A preparation method of veterinary ephedra, apricot kernel, gypsum and licorice oral liquid is characterized by comprising the following steps: the method comprises the following steps:
Taking 500 parts by weight of ephedra 300-;
taking the ephedra herb with the formula amount in the step (1), crushing the ephedra herb, adding purified water for soaking, inoculating a fermentation strain for fermentation after soaking thoroughly, wherein the inoculation amount is 8-12%, the fermentation time is 15h, and the fermentation temperature is 35-40 ℃;
Performing normal-pressure decoction extraction on the ephedra herb in the step (2) after strain fermentation, wherein the extraction temperature is 80-90 ℃, the material-liquid ratio is 1:8-1:12, repeating the extraction for 2 times, and the extraction time is 40min each time to obtain an ephedra herb extracting solution, filtering the ephedra herb extracting solution, and concentrating the ephedra herb to 500mL for later use;
Taking the bitter apricot seeds, the gypsum and the liquorice in the formula amount in the step (1) to respectively carry out normal pressure decoction extraction, wherein the water adding amount is 8-10 times, the decoction temperature is 95-100 ℃, the extraction time is 45min, extracting solutions of the medicinal materials are respectively obtained, and after filtration, the extracting solutions are respectively concentrated to 500mL of 300-;
And (4) mixing the concentrated solutions obtained in the steps (3) and (4), continuously concentrating to 1300mL of 1000-.
2. the preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (2), the fermentation strain is a fermentation strain with a preservation number of CCTCC NO: m2015455 Acetobacter orientalis.
3. The preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (2), the inoculation amount is 10%.
4. the preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (2), the fermentation temperature is 38 ℃.
5. The preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (3), the extraction temperature is 85 ℃.
6. the preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (3), the ratio of the material to the liquid is 1: 10.
7. the preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (4), the decoction temperature is 100 ℃.
8. the preparation method of the veterinary ephedra, apricot kernel, gypsum and licorice oral liquid as claimed in claim 1, is characterized by comprising the following steps: in the step (4), the filter element used for filtering is a PP folded filter element, and the filtering aperture is 0.44 μm.
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Cited By (2)
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|---|---|---|---|---|
| CN112608855A (en) * | 2020-10-30 | 2021-04-06 | 中国热带农业科学院海口实验站 | Compound microbial fertilizer and preparation method and application thereof |
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| CN116421653A (en) * | 2023-05-24 | 2023-07-14 | 河北维尔利生物科技有限公司 | Preparation process of ephedra, apricot kernel, gypsum and licorice oral liquid |
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