CN103463257A - Fructus aurantii immaturus or fructus aurantii extract, its preparation method and application - Google Patents
Fructus aurantii immaturus or fructus aurantii extract, its preparation method and application Download PDFInfo
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Abstract
The invention discloses a fructus aurantii immaturus or fructus aurantii extract, its preparation method and application. The fructus aurantii immaturus or fructus aurantii extract is prepared by: crushing a whole fructus aurantii immaturus or fructus aurantii fruit, conducting water extraction or alcohol extraction 1-3 times, at the same time collecting volatile oil, performing filtration and combining the filtrate, adding or not adding an organic solvent to conduct extraction, then carrying out concentration to obtain extractum, and combining the extractum with volatile oil, thus obtaining the fructus aurantii immaturus or fructus aurantii extract. The extract can be applied in preparation of pharmaceutical compositions for relieving cough and reducing sputum, and can achieve good cough relieving and sputum reducing effects. The production method is simple, and the effective component extraction rate is high.
Description
Technical field
The present invention relates to technical field of Chinese medicines, particularly relate to a kind of Fructus Aurantii Immaturus or Fructus Aurantii extract and its preparation method and application.
Background technology
Fructus Aurantii Immaturus (Fructus aurantii immaturus) is rutaceae Citrus aurantium Linn. Citrus aurantium L., the young fruit of Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus) (Poncirus trifoliata (L.) Raf.) or fragrant circle (Citrus wilsonii Tanaka), be mainly used in stagnant stopping, heavily wait after feeling of fullness distending pain, dysentery.
The dry immature fruit that Fructus Aurantii (Fructus aurantii) is rutaceae Citrus aurantium Linn. Citrus aurantium L. and variety thereof, have the effect of regulating the flow of QI to ease the stomach, the stagnant relieving distension of row.
Fructus Aurantii Immaturus, Fructus Aurantii are conventional Chinese medicine, and the history that is applied to clinically cure the disease has had two thousand years, are widely used in the traditional Chinese medical science compound preparation of curing the disease.Once had pharmacological evaluation to show, the Fructus Aurantii Immaturus medicament can make the laboratory animal gastrointestinal smooth muscle shrink the rhythm and pace of moving things to be increased, but there is not yet Fructus Aurantii Immaturus, Fructus Aurantii cough-relieving, reduces phlegm and the report of relieving asthma aspect effective ingredient or effective ingredient and preparation thereof.
On research and utilization in the past, major part is all the compound preparation according to ancient times, inadequate to the research of monarch drug Fructus Aurantii Immaturus wherein or Fructus Aurantii itself, and result of use is often little desirable; To play curative effect to disease, need larger dosage, and onset time be longer.The method of its exploitation, be compound preparation, with other Chinese medicine configurations, use, have no folk prescription and use, especially aspect relieving cough and resolving phlegm.
Summary of the invention
Based on this, the purpose of this invention is to provide a kind of Fructus Aurantii Immaturus or Fructus Aurantii extract, this extract has the effect of relieving cough and resolving phlegm.
Concrete technical scheme is as follows:
The preparation method of a kind of Fructus Aurantii Immaturus or Fructus Aurantii extract, comprise the steps: Fructus Aurantii Immaturus or Fructus Aurantii all-fruit powder broken, water extraction or alcohol extraction 1-3 time, collect volatile oil simultaneously, filter merging filtrate, add or do not add organic solvent extraction, then be condensed into extractum and volatile oil and merge, obtain Fructus Aurantii Immaturus or Fructus Aurantii extract.
Water extraction can adopt in room temperature or under the ebuillition of heated condition and carry out, and extraction time is 1-3 time, and the consumption of water is that water at least can not have medical material, and water consumption is not too much limit.
Alcohol extraction (ethanol extraction) is carried out at ambient temperature, and extraction time is 1-3 time, and the consumption of alcohol is not at least having medical material, and the ethanol consumption is not too much limit.Ethanol can adopt dehydrated alcohol or various concentration ethanol.
In some embodiment, the 3-20 that in described water extraction process, the consumption of water is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly therein; Described alcohol extraction process adopts ethanol as solvent, and the 3-20 that the consumption of described ethanol is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly.
In some embodiment, the 5-8 that in described water extraction process, the consumption of water is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly therein; Described alcohol extraction process ethanol is as solvent, and the 5-15 that the consumption of described ethanol is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly.
In some embodiment, described ethanol is the alcoholic solution that dehydrated alcohol or volumetric concentration are 60-90% therein.
In some embodiment, described organic solvent is chloroform, ethyl acetate or petroleum ether therein, and described petroleum ether is petroleum ether-30, petroleum ether-60 or petroleum ether-90.
Another object of the present invention is to provide Fructus Aurantii Immaturus or the Fructus Aurantii extract that above-mentioned preparation method prepares.
Concrete technical scheme is as follows:
Fructus Aurantii Immaturus or Fructus Aurantii extract that above-mentioned Fructus Aurantii Immaturus or Fructus Aurantii extract preparation method prepare.
Another object of the present invention is to provide Fructus Aurantii Immaturus that above-mentioned preparation method obtains or the application of Fructus Aurantii extract.
Concrete technical scheme is as follows:
The application in the pharmaceutical composition of preparation treatment relieving cough and resolving phlegm of the Fructus Aurantii Immaturus that above-mentioned preparation method obtains or Fructus Aurantii extract.
Another object of the present invention is to provide a kind of pharmaceutical composition.
Concrete technical scheme is as follows:
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect, its active component comprises Fructus Aurantii Immaturus and/or the Fructus Aurantii extract that above-mentioned preparation method prepares.
In some embodiment, the dosage form of this pharmaceutical composition is tablet, capsule, granule, oral liquid or aerosol therein.
Advantage of the present invention is as follows:
1, the present invention uses Fructus Aurantii Immaturus or Fructus Aurantii as folk prescription, Fructus Aurantii Immaturus and/or Fructus Aurantii extract is applied to treat to the medicine of relieving cough and resolving phlegm, possesses good relieving cough and resolving phlegm effect.
2, the present invention has the pharmacodynamic experiment of the pharmaceutical composition of relieving cough and resolving phlegm effect, at Zhongshan University's medical college zoopery center, carry out, pharmaceutical composition of the present invention is carried out to the relieving cough and resolving phlegm experiment of laboratory animal white mice, result shows: the basic, normal, high dosage gastric infusion of pharmaceutical composition of the present invention is to stimulating mice to cause the tolerance time of cough, with the blank group, compare, significant prolongation is all arranged, and there were significant differences statistically.
3, the pharmaceutical composition that the present invention has a relieving cough and resolving phlegm effect shows the experiment of reducing phlegm of laboratory animal mice, to mouse bronchial, secretion increases the basic, normal, high dosage gastric infusion of pharmaceutical composition of the present invention, with the blank group, compare, remarkable increase is all arranged, and there were significant differences statistically.Compare with positive control medicine TANKEJING, the mouse bronchial juice is all had to remarkable increase, there were significant differences statistically.The pharmacological effect effect of pointing out pharmaceutical composition of the present invention to there is relieving cough and resolving phlegm, and better than positive control curative effect.
4, Fructus Aurantii Immaturus or Fructus Aurantii extract and pharmaceutically acceptable adjuvant are made to the medicine of the dosage forms such as tablet, capsule, granule, oral liquid or aerosol, the medicine of these dosage forms has following characteristics:
(1) convenient drug administration, the patient can complete voluntarily; (2) dose is little, and curative effect is rapid; (3) easy to carry; (4) quality is more stable.
To sum up, pharmaceutical composition of the present invention adopts Fructus Aurantii Immaturus or the full medicinal raw material of really making a living of Fructus Aurantii, possesses good relieving cough and resolving phlegm result, and its production method is simple, and drug effect is obvious.
The specific embodiment
Raw material used in the present invention is as follows:
Fructus Aurantii Immaturus (Fructus aurantii immaturus) is rutaceae Citrus aurantium Linn. Citrus aurantium L., the young fruit of Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus) (Poncirus trifoliata (L.) Raf.) or fragrant circle (Citrus wilsonii Tanaka).
The dry immature fruit that Fructus Aurantii (Fructus aurantii) is rutaceae Citrus aurantium Linn. Citrus aurantium L. and variety thereof.
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect of the present embodiment is that active component and pharmaceutically acceptable adjuvant form by the Fructus Aurantii Immaturus water extract.
Preparation method is as follows:
(1) prepare the Fructus Aurantii Immaturus water extract:
The Fructus Aurantii Immaturus all-fruit powder is broken, crosses 20 mesh sieves, adds 100 ℃ of water and does not have medical material (consumption of water is 5 times of immature bitter orange raw material medicine volume), extracts 2 times, each 1 hour, filter, collect volatile oil, merging filtrate simultaneously, be condensed into extractum and volatile oil and merge, obtain the Fructus Aurantii Immaturus water extract, extraction ratio is 30% left and right approximately.
(2) the Fructus Aurantii Immaturus water extract and the conventional adjuvant that step (1) are obtained are made oral liquid.
Embodiment 2
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect of the present embodiment is that active component and pharmaceutically acceptable adjuvant form by the Fructus Aurantii water extract.
Preparation method is as follows:
(1) prepare the Fructus Aurantii water extract:
The Fructus Aurantii all-fruit powder is broken, cross 20 mesh sieves, add 100 ℃ of water and do not have medical material (consumption of water is 8 times of Fructus Aurantii crude drug volume), extract each 1 hour 3 times, filter, collect volatile oil, merging filtrate, then be condensed into extractum and volatile oil and merge simultaneously, obtain the Fructus Aurantii water extract, extraction ratio is 30% left and right approximately.
(2) Fructus Aurantii water extract step (1) obtained and conventional adjuvant are made tablet or capsule or granule or oral liquid.
Embodiment 3
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect of the present embodiment, be that active component and pharmaceutically acceptable adjuvant form by Fructus Aurantii Immaturus and Fructus Aurantii water extract, and wherein the amount ratio of Fructus Aurantii Immaturus water extract and Fructus Aurantii water extract is 1:1.
Preparation method is as follows:
(1) prepare Fructus Aurantii Immaturus or Fructus Aurantii water extract:
Fructus Aurantii Immaturus or Fructus Aurantii all-fruit powder are broken, cross 20 mesh sieves, add 100 ℃ of water and do not have medical material (consumption of water is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume 5 times), extract each 1 hour 3 times, filter, collect volatile oil, merging filtrate, then be condensed into extractum and volatile oil and merge simultaneously, obtain Fructus Aurantii Immaturus or Fructus Aurantii water extract, extraction ratio is 30% left and right approximately.
(2) Fructus Aurantii Immaturus step (1) obtained and Fructus Aurantii water extract are made tablet or capsule or granule or oral liquid with conventional adjuvant after mixing in the 1:1 ratio.
Embodiment 4
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect of the present embodiment is that active component and pharmaceutically acceptable adjuvant form by the Fructus Aurantii Immaturus alcohol extract.
Preparation method is as follows:
(1) prepare the Fructus Aurantii Immaturus alcohol extract:
The Fructus Aurantii Immaturus all-fruit powder is broken, without sieving, the alcoholic solution that to add volume content be 60% did not have medical material (consumption of alcoholic solution is 15 times of immature bitter orange raw material medicine volume), extracted 2 times, each 1 day, filter, collect volatile oil, merging filtrate simultaneously, add organic solvent (chloroform) extraction, then be condensed into extractum and volatile oil and merge, obtain the Fructus Aurantii Immaturus alcohol extract, extraction ratio is 30% left and right approximately.
(2) Fructus Aurantii Immaturus alcohol extract step (1) obtained and conventional adjuvant are made tablet or capsule or granule.
Embodiment 5
A kind of pharmaceutical composition with relieving cough and resolving phlegm effect of the present embodiment is that active component and pharmaceutically acceptable adjuvant form by the alcohol extract of Fructus Aurantii.
Preparation method is as follows:
(1) prepare the Fructus Aurantii alcohol extract:
The Fructus Aurantii all-fruit powder is broken, without sieving, the alcoholic solution that to add volume content be 90% did not have medical material (consumption of alcoholic solution is 5 times of immature bitter orange raw material medicine volume), extracted 2 times, each 1 day, filter, collect volatile oil, merging filtrate simultaneously, add organic solvent (ethyl acetate or petroleum ether-60) extraction, then be condensed into extractum and volatile oil and merge, obtain the Fructus Aurantii alcohol extract, extraction ratio is 30% left and right approximately.
(2) Fructus Aurantii alcohol extract step (1) obtained and conventional adjuvant are made tablet or capsule or granule.
Embodiment 6
The antitussive pharmacological research of pharmaceutical composition of the present invention
1, laboratory animal: the NIH mice, male and female half and half, clean grade standard, totally 40, by the sequence of body weight size, select the healthy animal of body weight 18～22g, be divided at random four groups, 10 every group.If negative control, positive control, Fructus Aurantii Immaturus water extract, the experimental grouies such as Fructus Aurantii water extract.
2, sample source and preparation:
(1) pharmaceutical composition that embodiment 1 prepares: Fructus Aurantii Immaturus water extract aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(2) pharmaceutical composition that embodiment 2 prepares: Fructus Aurantii water extract aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(3) dromethan: tablet, dromethan content is the 15mg/ sheet.Get 2 and be dissolved in 20 ml physiological salines, make suspension, concentration is 3mg/ml, and dosage is the 30mg/kg body weight.
(4) normal saline (sodium chloride injection), NaCl content 0.9%.
3, experimental technique: (strong aqua ammonia nebulization)
After mouse stomach 1 hour, start to accept the strong aqua ammonia spraying.Spray into the strong aqua ammonia aerosol by certain hour, spraying finishes, and takes out immediately mice, observes and has or not the cough reaction.Observe in one minute the number of times of coughing, if occur typical case's cough action (abdominal muscle shrinks or the contracting breast, magnifies mouth, sometimes can cough sound) person more than 3 times in 1 minute simultaneously, can be regarded as " cough is arranged ".Otherwise can be regarded as " without cough ".
4, experimentation:
Obtain with sequential method (upper purgation) spray time (EDT that causes the half mouse cough
50).Calculate the R value, if the R value is greater than 130%, illustrate that medicine has antitussive action.If the R value is greater than 150%, show to have significant antitussive action.Computing formula is as follows:
EDT
50=log
-1in the c/n(formula, n is number of animals, the summation that c is the rx value, the number of animals that r is every dosage group, the logarithm that x is dosage (being spray time).）
5, experimental result
By statistics, Fructus Aurantii Immaturus and Fructus Aurantii extract and half Cough length and cough suppressing effect see the following form 1.
Embodiments of the invention 1 Fructus Aurantii Immaturus water extract and embodiment 2 Fructus Aurantii water extract, gastric infusion is to stimulating mice to cause that the tolerance time of cough compares, embodiment 1 Fructus Aurantii Immaturus water extract and embodiment 2 Fructus Aurantii water body things have significant prolongation to the mice tolerance time, and there were significant differences statistically.
The cough suppressing effect of each sample of table 1
Embodiment 7
The antitussive pharmacological research of different dosing dosage
1, laboratory animal: the NIH mice, male, body weight 18.0-23.1g, clean grade standard, totally 75.Be divided at random five groups, 15 every group.If negative control, high, medium and low three dosage groups of positive control and Fructus Aurantii Immaturus water extract.
2, sample source and configuration:
(1) embodiment 1 prepares pharmaceutical composition: Fructus Aurantii Immaturus water extract aqueous solution, amounting to crude drug content is 350mg/ml.
(2) the direct administration of the pharmaceutical composition of high dose group: embodiment 1 (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg.kg body weight).
(3) dosage group in: the pharmaceutical composition by embodiment 1 adds normal saline, by administration (be equivalent to crude drug content 117mg/ml, dosage is equivalent to crude drug 1167mg/kg body weight) after the 1:2 dilution.
(4) low dose group: the pharmaceutical composition by embodiment 1, add normal saline, press the 1:8 dilution, administration (be equivalent to crude drug content 38.9mg/ml, dosage is equivalent to crude drug 389mg/kg body weight) after dilution.
(5) positive thing adopts dromethan: tablet, dromethan content is the 15mg/ sheet.Get 2 and be dissolved in 20 ml physiological salines, make suspension, concentration is 3mg/ml, and dosage is the 30mg/kg body weight.
3, experimental technique: with embodiment 6.
4, experimental result:
By statistics, each dosage group half Cough length and cough suppressing effect see the following form 2.
The cough suppressing effect of table 2 Fructus Aurantii Immaturus the water extracted immersing paste
Embodiment 8
Different cough-relieving experiments of extracting composition
1, laboratory animal: the NIH mice, male, body weight 18.0-23.1g, clean grade standard, totally 105.Be divided at random six groups, 15 every group.If negative control, positive control and Fructus Aurantii alcohol extract (petroleum ether) aqueous solution group; Fructus Aurantii Immaturus alcohol extract (chloroform) aqueous solution group; Fructus Aurantii alcohol extract (ethyl acetate) aqueous solution group; Fructus Aurantii Immaturus water extraction aqueous solution group.
2, sample source and preparation:
(1) embodiment 5 petroleum ether extractiones become grouping: Fructus Aurantii alcohol extract (petroleum ether) aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(2) embodiment 4 chloroform extractions become grouping: Fructus Aurantii Immaturus alcohol extract (chloroform) aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(3) embodiment 5 ethyl acetate extraction component groups: Fructus Aurantii alcohol extract (ethyl acetate) aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(4) embodiment 1 water extracting component group: Fructus Aurantii Immaturus water extraction aqueous solution (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg/kg body weight).
(5) positive thing adopts dromethan: tablet, dromethan content is the 15mg/ sheet.Get 2 and be dissolved in 20 ml physiological salines, make suspension, concentration is 3mg/ml, and dosage is the 30mg/kg body weight.
(6) normal saline (sodium chloride injection), NaCl content 0.9%.
3, experimental technique: with embodiment 7, adopt the strong aqua ammonia nebulization.
4, experimental result:
By statistics, respectively be extracted into grouping half Cough length and cough suppressing effect and see the following form 3.
Fructus Aurantii Immaturus of the present invention or Fructus Aurantii extract extractum and through the separating obtained extractum of various organic solvents, gastric infusion, to stimulating mice to cause the tolerance time and the comparison of blank group of cough, all has significant prolongation, and there were significant differences statistically.Contrast the dromethan sheet with positive drug relatively, tolerance time extends, and curative effect is remarkable than positive drug group dromethan.
Table 3. Fructus Aurantii Immaturus or Fructus Aurantii are respectively extracted the cough suppressing effect of composition
Embodiment 9
The apophlegmatisant pharmacological research of different dosing dosage
1, laboratory animal: the NIH mice, male, body weight 18.0-23.1g, clean grade standard, totally 75.Be divided at random five groups, 15 every group.If negative control, high, medium and low three dosage groups of positive control and Fructus Aurantii Immaturus water extract.All by oral administration of gavage amount of 10ml/kg body weight.
2, sample source and preparation:
(1) pharmaceutical composition that embodiment 1 prepares: Fructus Aurantii Immaturus water extract aqueous solution, amounting to crude drug content is 350mg/ml.
(2) high dose group: embodiment 1 obtains the direct administration of pharmaceutical composition (be equivalent to crude drug content 350mg/ml, dosage is equivalent to crude drug 3500mg.kg body weight).
(3) pharmaceutical composition that in, dosage group: embodiment 1 obtains, add normal saline, by administration (be equivalent to crude drug content 117mg/ml, dosage is equivalent to crude drug 1167mg/kg body weight) after the 1:2 dilution.
(4) pharmaceutical composition that low dose group: embodiment 1 obtains, add normal saline, presses the 1:8 dilution, administration (be equivalent to crude drug content 38.9mg/ml, dosage is equivalent to crude drug 389mg/kg body weight) after dilution.
(5) positive thing adopts TANKEJING: powder, and get 0.2 gram and be dissolved in 10 ml physiological salines, obtain positive control TANKEJING solution, concentration is 20mg/ml.Dosage is the 200mg/kg body weight.
(6) normal saline (sodium chloride injection), NaCl content 0.9%.
3, experimental technique: (phenol red method)
(1) water 12h is can't help in the mice fasting.
(2) gastric infusion.Press the animal order, stop after every mouse stomach 3 minutes, then fill with other one, interval is 3 minutes, and 10 every group the gavage time is 30 minutes altogether.
(3) half an hour after each Mus gavage, through the phenol red normal saline solution 0.2ml of lumbar injection 5%.In order, i.e. phenol red latter 3 minutes of each mouse peritoneal injection, then inject other, 10 mices totally 30 minutes.
(4) half an hour after each Mus lumbar injection, de-cervical vertebra is put to death mice in order, puts to death interval 3 minutes.After sacrifice of animal, face upward position and be fixed on the operation plate, cut off neck center skin, separate trachea, with pincet, prop trachea.
(5) draw normal saline flushing trachea outer wall with large syringe, wash away phenol red in blood and trachea outer wall, filter paper blots washing liquid.
(6) cut trachea prior to the trachea bifurcation, then cut trachea (the ring-type thyroid cartilage is included) in other end thyroid cartilage upper end.
(7) each trachea section is put into to 5% the NaHCO that fills in advance 1.5ml
3in the solution test tube.
(8) completed above-mentioned tracheorrhaphy from shearing work in 3 minutes, then process second mice with same method, method as above.
(9) each test tube is put on vortex mixer and shaken 2 minutes, make phenol red the discharging in the trachea section.
(10) add 1MNaOH solution 0.1ml before the detection, centrifugal (3000rpm, 5min), survey the OD values by solution in each test tube in 721 type spectrophotometer 546nm places.
(11) according to the phenol red excretion amount of regression equation calculation escape pipe.Computing formula: y=0.119x-0.0059.X is the OD value, and y is the phenol red excretion amount of trachea.
4, result judgement:
Each group result is carried out to variance analysis, when overall when variant, if positive drug and matched group relatively, are proofreaied and correct phenol red content and raise, and significant difference (P<0.05) is arranged, determine that experiment reliably.By each dosage group and matched group relatively, proofread and correct phenol red content and obviously raise again, and when significant difference (P<0.05) is arranged, think that this dosage is effective.
5, experimental result:
By statistics, each dosage group of water extract extractum phenol red output, see the following form 4.
The cough suppressing effect of each dosage group of table 4 Fructus Aurantii Immaturus water extract
The phenol red excretion amount of trachea computational methods (ug/ml): OD value * 0.119-0.0059
Pharmaceutical composition of the present invention shows the experiment of reducing phlegm of laboratory animal mice, the basic, normal, high dosage gastric infusion of pharmaceutical composition of the present invention increases with the blank group and compares the mouse bronchial juice, remarkable increase is all arranged, and there were significant differences statistically.With the positive drug TANKEJING, compare, embodiment is high, middle, and the dosage group has remarkable increase to the mouse bronchial juice, and there were significant differences statistically.Show, embodiment 1 effect of reducing phlegm is better than the positive control TANKEJING.Through test, other embodiment of the present invention show to possess similar effect to embodiment 1.
In sum, illustrate that Fructus Aurantii Immaturus of the present invention or Fructus Aurantii extract have good relieving cough and resolving phlegm effect.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (9)
1. the preparation method of a Fructus Aurantii Immaturus or Fructus Aurantii extract, it is characterized in that, comprise the following steps: Fructus Aurantii Immaturus or Fructus Aurantii all-fruit powder is broken, water extraction or alcohol extraction 1-3 time, collect volatile oil simultaneously, filter merging filtrate, add or do not add organic solvent extraction, then be condensed into extractum and volatile oil and merge, obtain Fructus Aurantii Immaturus or Fructus Aurantii extract.
2. the preparation method of Fructus Aurantii Immaturus according to claim 1 or Fructus Aurantii extract, is characterized in that, the 3-20 that in described water extraction process, the consumption of water is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly; Described alcohol extraction process ethanol is as solvent, and the 3-20 that the consumption of described ethanol is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly.
3. the preparation method of Fructus Aurantii Immaturus according to claim 2 or Fructus Aurantii extract, is characterized in that, the 5-8 that in described water extraction process, the consumption of water is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly; Described alcohol extraction process ethanol is as solvent, and the 5-15 that the consumption of described ethanol is Fructus Aurantii Immaturus or Fructus Aurantii crude drug volume doubly.
4. according to the preparation method of the described Fructus Aurantii Immaturus of claim 2 or 3 or Fructus Aurantii extract, it is characterized in that, described ethanol is the alcoholic solution that dehydrated alcohol or volumetric concentration are 60-90%.
5. according to the described Fructus Aurantii Immaturus of claim 1-3 any one or Fructus Aurantii extract preparation method method, it is characterized in that, described organic solvent is chloroform, ethyl acetate or petroleum ether, and described petroleum ether is petroleum ether-30, petroleum ether-60 or petroleum ether-90.
6. the Fructus Aurantii Immaturus or the Fructus Aurantii extract that by the described preparation method of claim 1-5 any one, are obtained.
7. the application in the pharmaceutical composition of preparation treatment relieving cough and resolving phlegm of the Fructus Aurantii Immaturus that the described preparation method of claim 1-5 any one obtains or Fructus Aurantii extract.
8. the pharmaceutical composition with relieving cough and resolving phlegm effect, is characterized in that, its active component comprises Fructus Aurantii Immaturus and/or the Fructus Aurantii extract that the described preparation method of claim 1-5 any one obtains.
9. the pharmaceutical composition with relieving cough and resolving phlegm effect according to claim 8, is characterized in that, the dosage form of this pharmaceutical composition is tablet, capsule, granule, oral liquid or aerosol.
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| CN109363476A (en) * | 2017-01-18 | 2019-02-22 | 福建兴全香业有限公司 | Environmentally friendly Buddhist perfume (or spice) and preparation method thereof |
| CN112546194A (en) * | 2021-02-05 | 2021-03-26 | 青岛王晨龙中医研究所 | Oral liquid for relieving chest stuffiness and regulating qi |
| CN114432377A (en) * | 2022-02-18 | 2022-05-06 | 江西农业大学 | Application of traditional Chinese medicine immature bitter orange ethanol extract in preparation of disinfection product |
| CN114470052A (en) * | 2022-02-18 | 2022-05-13 | 江西农业大学 | Application of traditional Chinese medicine immature bitter orange ethanol extract in preparation of antibacterial drugs |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105641148A (en) * | 2016-02-26 | 2016-06-08 | 辽宁中医药大学 | Application of bitter orange volatile oil extract in preparation of antidepressant drug |
| CN105641148B (en) * | 2016-02-26 | 2019-09-17 | 辽宁中医药大学 | Application of the Fructus Aurantii volatile oil extract as preparation antidepressant |
| CN109363476A (en) * | 2017-01-18 | 2019-02-22 | 福建兴全香业有限公司 | Environmentally friendly Buddhist perfume (or spice) and preparation method thereof |
| CN112546194A (en) * | 2021-02-05 | 2021-03-26 | 青岛王晨龙中医研究所 | Oral liquid for relieving chest stuffiness and regulating qi |
| CN114432377A (en) * | 2022-02-18 | 2022-05-06 | 江西农业大学 | Application of traditional Chinese medicine immature bitter orange ethanol extract in preparation of disinfection product |
| CN114470052A (en) * | 2022-02-18 | 2022-05-13 | 江西农业大学 | Application of traditional Chinese medicine immature bitter orange ethanol extract in preparation of antibacterial drugs |
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Application publication date: 20131225 |