CN1927857A - Extraction and purification process of buckwheat sprout total flavone - Google Patents
Extraction and purification process of buckwheat sprout total flavone Download PDFInfo
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- CN1927857A CN1927857A CN 200610113363 CN200610113363A CN1927857A CN 1927857 A CN1927857 A CN 1927857A CN 200610113363 CN200610113363 CN 200610113363 CN 200610113363 A CN200610113363 A CN 200610113363A CN 1927857 A CN1927857 A CN 1927857A
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- buckwheat
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- total flavone
- buckwheat sprout
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Abstract
The present invention relates to flavone extracting technology, and is especially buckwheat seedling flavone extracting and refining process. The buckwheat seedling flavone extracting and refining process includes the steps of pre-treatment of buckwheat seedling, soaking in alcohol solution, concentrating the extracted liquid to form paste, diluting with deionized water, resin adsorbing, alcohol eluting, concentrating, drying, etc. The process of the present invention is superior to available processes, and has the advantages of high flavone extracting rate and stable and controllable quality, and the product can meet the quality requirement for use as medicine and food additive, etc.
Description
Technical field the present invention relates to a kind of extracting method of flavonoid compound, promptly a kind of extraction and purification process of buckwheat sprout total flavone.
The background technology total flavones is the general name of flavonoid compound, wherein multiple material such as rutin all has pharmaceutical use, particularly the effect of vessel softening is more outstanding, has the good curing effect for diseases such as hyperlipidemia, cerebrovascular sclerosis, capillary blood vessel are fragile hemorrhage.At present, the raw material and the method that are used for producing total flavones have many kinds, and wherein, the sub-reality of buckwheat and the root of buckwheat plant, stem, leaf all are the raw materials that is rich in total flavones.In recent years, the research that relevant Buckwheat seedling extracts total flavones also has a small amount of report, but complete processing is only limited to two kinds of breaking and taking method and drying milling methods.Breaking and taking method is with after the fresh Buckwheat seedling crushing and beating, separates by centrifugal realization screenings, again the screenings concentrate drying promptly.Drying milling method is pulverizing after the Buckwheat seedling oven dry or carrying out the ultra micro efflorescence.The product Flavonoid substances content that these methods are produced is lower, and contains impurity such as amounts of protein, carbohydrate, and difficult quality control is difficult to realize large-scale commercial production, has limited the application of product.
Summary of the invention the purpose of this invention is to provide a kind of suitable large-scale commercial production, and the product flavones content is higher, extraction and purification process of buckwheat sprout total flavone stable and controllable for quality.
Above-mentioned purpose is realized by following technical scheme: will make extracting solution through alcohol solution dipping through pretreated Buckwheat seedling, and extracting solution be concentrated receive cream, and with the deionized water dilution, use resin absorption, and use ethanol elution, the reconcentration drying makes.
Said buckwheat comprises sweet buckwheat and bitter buckwheat, and its Buckwheat seedling is chosen 10 days over-ground part of SPRING WHEAT BEFORE AND AFTER FLOWERING as raw material.
Said Buckwheat seedling preprocessing process is that water after the segment of buckwheat cauline leaf is cleaned, and dries, dry for standby again.
Said Buckwheat seedling preprocessing process is that water after the segment of buckwheat cauline leaf is cleaned, and is broken more standby.
Said alcohol solution dipping process is that the aqueous ethanolic solution of standby raw material with 50-95% soaked 2-10 days, extracts 2-3 time, makes extracting solution.
Said alcohol solution dipping process be with standby raw material with the aqueous ethanolic solution reflux of 50-95% 1-2 hour, extract 2-3 time, make extracting solution.
Said receipts cream process is that extracting solution is received cream through the parting liquid that centrifugation obtains at 50-70 ℃ of following vacuum concentration.
Said receipts cream process is that extracting solution is received cream through filtering the filtered liquid that obtains at 50-70 ℃ of following vacuum concentration.
Again gained medicinal extract is diluted with 1-3 deionized water doubly after the said receipts cream process, pass through macroporous resin adsorption then, and use ethanol gradient elution successively, make medicinal extract after elutriant is concentrated once more, and adopt vacuum lyophilization or vacuum-drying below 30-60 ℃ to make extractive of general flavone.
Adopt above-mentioned technology, flavones extraction yield height, stable and controllable for quality, can satisfy the specification of quality of aspects such as medicine, foodstuff additive, and method is easy, with low cost, be suitable for large-scale industrial production, thereby provide new approach for the development and use of buckwheat resource.
Introduce three kinds of embodiment below the embodiment:
First kind of embodiment: bitter buckwheat was emerged 20 days the time, extract Buckwheat seedling 1000kg from about 4cm more than the ground, be cut into the segment about 6cm, get after drying naturally about dry product 18kg, add 180 liter of 70% ethanol then extractor internal heating refluxing extraction 3 times, each 1 hour, merge behind the extracting liquid filtering, carry out vacuum concentration and receive cream, get 20 liters of medicinal extract, with the medicinal extract lyophilize, pulverize promptly to get and combine yellow dry extract 1.5kg again, its general flavone content reaches 30%.
Second kind of embodiment: sweet buckwheat was emerged 25 days the time, extracted Buckwheat seedling 1000kg from about 4cm more than the ground, was cut into the segment about 6cm, place in the stainless steel storage tank, add 80% ethanol 200kg, soak at room temperature 7 days soaks 2 times, filter merging filtrate then, concentrate and obtain 18 liters of medicinal extract, medicinal extract 45 ℃ of vacuum-dryings, is pulverized, obtain dried powder 1.3kg, recording general flavone content is 27%.
The third embodiment: bitter buckwheat was emerged 20 days the time, extract Buckwheat seedling 1000kg from about 4cm more than the ground, be cut into the segment about 6cm, naturally get about dry product 18kg after drying, add 180 liter of 70% ethanol then extractor internal heating refluxing extraction 3 times, each 1 hour, merge behind the extracting liquid filtering, carry out vacuum concentration and receive cream, get 70 liters of medicinal extract, again medicinal extract is used 70% ethanol elution, collect 70% ethanol eluate, vacuum concentration is received cream, and lyophilize gets extractive total flavone content and reaches more than 50%.
Claims (10)
1. an extraction and purification process of buckwheat sprout total flavone is characterized in that: will make extracting solution through alcohol solution dipping through pretreated Buckwheat seedling, and extracting solution be concentrated receive cream, dilute with deionized water, use resin absorption, and use ethanol elution, the reconcentration drying makes.
2. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said buckwheat comprises sweet buckwheat and bitter buckwheat, and its Buckwheat seedling is chosen 10 days over-ground part of SPRING WHEAT BEFORE AND AFTER FLOWERING as raw material.
3. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said Buckwheat seedling preprocessing process is that water after the segment of buckwheat cauline leaf is cleaned, and dries, dry for standby again.
4. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said Buckwheat seedling preprocessing process is that water after the segment of buckwheat cauline leaf is cleaned, and is broken more standby.
5. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said alcohol solution dipping process is that the aqueous ethanolic solution of standby raw material with 50-95% soaked 2-10 days, extracts 2-3 time, makes extracting solution.
6. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said alcohol solution dipping process be with standby raw material with the aqueous ethanolic solution reflux of 50-95% 1-2 hour, extract 2-3 time, make extracting solution.
7. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said receipts cream process is that extracting solution is received cream through the parting liquid that centrifugation obtains at 50-70 ℃ of following vacuum concentration.
8. extraction and purification process of buckwheat sprout total flavone according to claim 1 is characterized in that: said receipts cream process is that extracting solution is received cream through filtering the filtered liquid that obtains at 50-70 ℃ of following vacuum concentration.
9. extraction and purification process of buckwheat sprout total flavone according to claim 1, it is characterized in that: again gained medicinal extract is diluted with 1-3 deionized water doubly after the said receipts cream process, pass through macroporous resin adsorption then, and use ethanol gradient elution successively, make medicinal extract after elutriant concentrated once more, and adopt vacuum lyophilization or vacuum-drying below 30-60 ℃ to make extractive of general flavone.
10. extraction and purification process of buckwheat sprout total flavone according to claim 1, it is characterized in that: again gained medicinal extract is diluted with 1-3 deionized water doubly after the said receipts cream process, pass through macroporous resin adsorption then, and use ethanol gradient elution successively, make medicinal extract after elutriant concentrated once more, and adopt vacuum lyophilization or vacuum-drying below 30-60 ℃ to make extractive of general flavone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610113363 CN1927857A (en) | 2006-09-25 | 2006-09-25 | Extraction and purification process of buckwheat sprout total flavone |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610113363 CN1927857A (en) | 2006-09-25 | 2006-09-25 | Extraction and purification process of buckwheat sprout total flavone |
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| CN1927857A true CN1927857A (en) | 2007-03-14 |
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| CN 200610113363 Pending CN1927857A (en) | 2006-09-25 | 2006-09-25 | Extraction and purification process of buckwheat sprout total flavone |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210388A (en) * | 2011-07-27 | 2011-10-12 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN102641343A (en) * | 2012-05-05 | 2012-08-22 | 耿福能 | Water-soluble tartary buckwheat general flavone extract and preparation method and application thereof |
| CN102948758A (en) * | 2012-11-26 | 2013-03-06 | 陕西科技大学 | Method for extracting buckwheat flavone from buckwheat bran |
| CN103284163A (en) * | 2012-03-02 | 2013-09-11 | 侯兆乾 | Subcritical extraction method of flavone in tartary buckwheat steams and leaves |
| CN104323383A (en) * | 2014-10-06 | 2015-02-04 | 李发旺 | Tartary buckwheat plant beverage |
| CN105056570A (en) * | 2014-08-10 | 2015-11-18 | 胡刘满 | System for rapidly extracting flavone from rhododendron simsii planch leaves |
| CN109369733A (en) * | 2018-10-30 | 2019-02-22 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of flavone compounds simultaneously from leaf of Radix Et Rhizoma Fagopyri Tatarici |
-
2006
- 2006-09-25 CN CN 200610113363 patent/CN1927857A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210388A (en) * | 2011-07-27 | 2011-10-12 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN102210388B (en) * | 2011-07-27 | 2012-07-04 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN103284163A (en) * | 2012-03-02 | 2013-09-11 | 侯兆乾 | Subcritical extraction method of flavone in tartary buckwheat steams and leaves |
| CN102641343A (en) * | 2012-05-05 | 2012-08-22 | 耿福能 | Water-soluble tartary buckwheat general flavone extract and preparation method and application thereof |
| CN102948758A (en) * | 2012-11-26 | 2013-03-06 | 陕西科技大学 | Method for extracting buckwheat flavone from buckwheat bran |
| CN102948758B (en) * | 2012-11-26 | 2014-07-30 | 陕西科技大学 | Method for extracting buckwheat flavone from buckwheat bran |
| CN105056570A (en) * | 2014-08-10 | 2015-11-18 | 胡刘满 | System for rapidly extracting flavone from rhododendron simsii planch leaves |
| CN104323383A (en) * | 2014-10-06 | 2015-02-04 | 李发旺 | Tartary buckwheat plant beverage |
| CN109369733A (en) * | 2018-10-30 | 2019-02-22 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of flavone compounds simultaneously from leaf of Radix Et Rhizoma Fagopyri Tatarici |
| CN109369733B (en) * | 2018-10-30 | 2020-07-07 | 湖南华诚生物资源股份有限公司 | Method for simultaneously extracting multiple flavonoid compounds from tartary buckwheat leaves |
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