CN100560653C - A kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment - Google Patents
A kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment Download PDFInfo
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- CN100560653C CN100560653C CNB2005100449488A CN200510044948A CN100560653C CN 100560653 C CN100560653 C CN 100560653C CN B2005100449488 A CNB2005100449488 A CN B2005100449488A CN 200510044948 A CN200510044948 A CN 200510044948A CN 100560653 C CN100560653 C CN 100560653C
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- enzymolysis
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Abstract
A kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment of the present invention comprises the steps: Red kojic rice → immersion alkaline hydrolysis → heating gelatinization → enzymolysis → filtering separation → Acid precipitation → washing → neutralization → drying → pulverizing → packing.The present invention utilizes enzymolysis process, make starch in the Red kojic rice change into the oligose or the glucose of solubility, help improving the solvability of monascorubin, the weave construction of Red kojic rice is decomposed, so that allow deliquescent monascorubin fully discharge, and then be easy to separate, both simplified extraction process with other insoluble components, can improve the extraction yield of monascorubin again effectively, the output of monascorubin can reach 15～25%.
Description
Technical field
The invention belongs to the foodstuff additive industrial circle, relate to a kind of extracting method of monascorubin, particularly a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment.
Background technology
Human use's red colouring agent for food, also used as a Chinese medicine has quite long history.Except being used for the brew red wine, China people utilize tinting material and the sanitas of red rice products as food already, as: preserved red beancurd, biltong, fish curing food, pickles etc.In recent years, adopt natural pigment to replace edible synthesized coloring matter and become trend of the times.
Monascorubin is a main raw material with grain, is the meta-bolites of Monascus anka Nakazawa et sato culturing process, and the basic raw material abundance can satisfy scale operation needs all the year round.China is the major country of production of red colouring agent for food, also used as a Chinese medicine, and is with a long history, technical maturity.Generally speaking, be raw material with the rice, the pigment content in the Red kojic rice can reach 10～20%, and the content of other natural pigments in raw material is then mostly below 3%.Because material content is higher, thereby helps reducing auxiliary material consumption and hold facility rate, reduces production costs.
The pigment that produces in the Monascus anka Nakazawa et sato metabolic process is a class alcohol dissolubility pigment, dissolves in ethanol, acetone and other organic solvent.The separating and extracting method of monascorubin directly influences the quality and benefits of its product and follow-up derived product, produce monascorubin at present and mainly adopt extraction method of enrichment or the direct method of enrichment of fermented liquid, complex process, product is difficult for dry, and purity is lower, and acid resistance is also bad.Adopt enzymolysis process to separate monascorubin from Red kojic rice, so that simplify technology, reduce material consumption, significantly improve product yield, this class research does not appear in the newspapers so far.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment.By the enzymatic treatment to Red kojic rice, enzymolysis starch wherein effectively decomposes the weave construction of Red kojic rice, so that allow deliquescent monascorubin fully discharge, and then separates with insoluble other components.
Technical process of the present invention is as follows: raw material, immersion alkaline hydrolysis, heating gelatinization, enzymolysis, filtering separation, Acid precipitation, washing, neutralization, drying, pulverizing, packing.The purity of used chemical reagent is food grade.
A kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment of the present invention may further comprise the steps:
(1) raw material: getting Red kojic rice is raw material.Described Red kojic rice is after rice ferments through routine, to obtain Red kojic rice.
(2) soak alkaline hydrolysis: Red kojic rice is added in the alkali lye, and temperature is controlled between 20～60 ℃, and the mass percent of Red kojic rice and alkali lye is 1: 1～1: 5, concentration of lye for O.01mol/L～0.1mol/L, soak time 30～200min; Described alkali is oxyhydroxide or carbonate or supercarbonate;
(3) heating gelatinization: temperature is risen to 100 ℃～150 ℃, and constant temperature 20～200min gets the Red kojic rice dextrin;
(4) enzymolysis: the Red kojic rice dextrin that step (3) is obtained carries out enzymolysis processing, and hydrolysis temperature is 40～75 ℃, and pH is 5～8, adds α-Dian Fenmei and carries out enzymolysis, and stir on enzymolysis limit, limit; The mass ratio of amylase and Red kojic rice dextrin is 1: 10000～5: 1000, and enzymolysis time is 0.3～8h, 10～100 rev/mins of stirring velocitys; Get enzymolysis solution behind the enzymolysis; Described acid is Hcl.
(5) filtering separation: enzymolysis solution is filtered, and solid-liquid separation gets filtrate; Described filtration can be adopted plate-and-frame filter press or vacuum filter or whizzer; Filter screen is more than 300 orders.
(6) Acid precipitation: in filtrate, add acid, regulate pH to 1～3, and leave standstill 2～10h, make its precipitation; Described acid is Hcl.
(7) washing: the precipitation that step (6) is obtained adds water washing to pH5, filters then, gets throw out;
(8) neutralization: the throw out that step (7) is obtained transfers to pH7～8 with alkali, and it is dissolved fully, becomes the monascorubin aqueous solution;
(9) drying: the monascorubin aqueous solution that step (8) is obtained carries out drying treatment, gets dry water-soluble monascus pigment; Described drying refers to that the water ratio of pigment is lower than 5%, and drying treatment can adopt spray-drier or tunnel drier or boiling drier or pneumatic drier.
(10) pulverize: the dry water-soluble monascus pigment powder that step (9) is obtained is crushed to the granularity that meets product requirement.
A kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment of the present invention, can also adopt following parameter:
1, the immersion alkaline hydrolysis described in the step (2), the temperature that Red kojic rice adds in the alkali lye is controlled between 40～50 ℃, and the mass percent of Red kojic rice and alkali lye is 1: 2～1: 4, soak time 30～120min.
2, the heating gelatinization described in the step (3), the temperature of heating gelatinization is controlled at 100 ℃～120 ℃, and constant temperature time is 20～60min.
3, the enzymolysis described in the step (4), hydrolysis temperature is 50～65 ℃, and pH is 6～7, and the mass ratio of amylase and Red kojic rice dextrin is 5: 10000～2: 1000, and enzymolysis time is 0.3～3h.
4, the Acid precipitation described in the step (6) adds acid in filtrate, and time of repose is 2～10h.
The present invention is a raw material with the Red kojic rice, and the pigment content in the Red kojic rice can reach 10～20%, and the content of other natural pigments in raw material is then mostly below 3%.Because material content is higher, thereby helps reducing auxiliary material consumption and hold facility rate, reduces production costs.And China is the major country of production of red colouring agent for food, also used as a Chinese medicine, and is with a long history, technical maturity, Red kojic rice wide material sources, raw material abundance.The present invention is by the enzymatic treatment to Red kojic rice, enzymolysis starch wherein effectively, make its oligose that changes into solubility or glucose, help improving the solvability of monascorubin, the weave construction of Red kojic rice is decomposed, so that allow deliquescent monascorubin fully discharge, and then be easy to separate with other insoluble components, both simplified extraction process, can improve the extraction yield of monascorubin again effectively, and made the output of monascorubin reach 15～25%.
The present invention is applicable to that various scales are raw material with the Red kojic rice, the production of Enzymatic Extraction water-soluble monascus pigment.
Embodiment
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment: with raw material is that 1 ton of Red kojic rice is an example, and the method for utilizing enzymolysis process to extract water-soluble monascus pigment may further comprise the steps:
(1) raw material: 1 ton of Red kojic rice; Can buy by market.
(2) soak alkaline hydrolysis: in 5 tons of 1 ton of adding of Red kojic rice 0.05mol/L NaOH alkali lye, temperature is controlled at 60 ℃, soak time 120min;
(3) heating gelatinization: temperature is risen to 100 ℃, and constant temperature 30min makes the Red kojic rice gelatinization.
(4) enzymolysis: add 6mol/L HCl and regulate pH to 7, temperature is controlled at is 60 ℃, adds α-Dian Fenmei 20kg then, enzymolysis 8h opens in the enzymolysis process and stirs, and stirring velocity is controlled at about 50 rev/mins;
(5) filtering separation: the enzyme processing is used the plate-and-frame filter press press filtration after finishing, and removes filter residue;
(6) Acid precipitation: in filtrate, add 6mol/L HCl, regulate pH to 2, make its precipitation, and leave standstill 6h;
(7) washing: the precipitation that step (6) is obtained adds water washing to pH5, and removes washings with the whizzer separation;
(8) neutralization: the precipitation that step (7) is obtained adds Na
2CO
3Be neutralized to pH8, be diluted to 500L, and fully stir,, get the monascorubin aqueous solution to dissolving fully;
(9) drying: the monascorubin aqueous solution spray-drier drying with step (8) obtains gets water-soluble monascus pigment powder 20～25kg;
(10) pulverize: the water-soluble monascus pigment powder that step (9) obtains is pulverized with pulverizing mill, made it meet the product granularity requirement.
Claims (5)
1, a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment is characterized in that comprising the steps:
(1) raw material: getting Red kojic rice is raw material;
(2) soak alkaline hydrolysis: Red kojic rice is added in the alkali lye, and temperature is controlled between 20～60 ℃, and the mass percent of Red kojic rice and alkali lye is 1: 1～1: 5, and concentration of lye is 0.01mol/L～0.1mol/L, soak time 30～200min;
(3) heating gelatinization: temperature is risen to 100 ℃～150 ℃, and constant temperature 20～200min gets the Red kojic rice dextrin;
(4) enzymolysis: the Red kojic rice dextrin that step (3) is obtained carries out enzymolysis processing, and hydrolysis temperature is 40～75 ℃, and pH is 5～8, adds α-Dian Fenmei and carries out enzymolysis, and stir on enzymolysis limit, limit; The mass ratio of amylase and Red kojic rice dextrin is 1: 10000～5: 1000, and enzymolysis time is 0.3～8h, 10～100 rev/mins of stirring velocitys; Get enzymolysis solution behind the enzymolysis;
(5) filtering separation: enzymolysis solution is filtered, and solid-liquid separation gets filtrate;
(6) Acid precipitation: in filtrate, add acid, regulate pH to 1～3, and leave standstill 2～10h, make its precipitation;
(7) washing: the precipitation that step (6) is obtained adds water washing to pH5, filters then, gets throw out;
(8) neutralization: the throw out NaCO that step (7) is obtained
3Transfer to pH7～8, it is dissolved fully, get the monascorubin aqueous solution;
(9) drying: the monascorubin aqueous solution that step (8) is obtained carries out drying treatment, gets dry water-soluble monascus pigment;
(10) pulverize: the dry water-soluble monascus pigment powder that step (9) is obtained is crushed to the granularity that meets product requirement.
2, a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment according to claim 1, it is characterized in that described enzymolysis, hydrolysis temperature is 50～65 ℃, and pH is 6～7, the mass ratio of amylase and Red kojic rice dextrin is 5: 10000～2: 1000, and enzymolysis time is 0.3～3h.
3, a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment according to claim 1, it is characterized in that described immersion alkaline hydrolysis, the temperature that Red kojic rice adds in the alkali lye is controlled between 40～50 ℃, the mass percent of Red kojic rice and alkali lye is 1: 2～1: 4, soak time 30～120min.
4, a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment according to claim 1 is characterized in that described heating gelatinization, and the temperature of heating gelatinization is controlled at 100 ℃～120 ℃, and constant temperature time is 20～60min.
5, a kind of method of utilizing enzymolysis process to extract water-soluble monascus pigment according to claim 1 is characterized in that may further comprise the steps:
(1) raw material: 1 ton of Red kojic rice;
(2) soak alkaline hydrolysis: in 5 tons of 1 ton of adding of Red kojic rice 0.05mol/L NaOH alkali lye, temperature is controlled at 60 ℃, soak time 120min;
(3) heating gelatinization: temperature is risen to 100 ℃, and constant temperature 30min makes the Red kojic rice gelatinization;
(4) enzymolysis: add 6mol/L HCl and regulate pH to 7, temperature is controlled at is 60 ℃, adds α-Dian Fenmei 20kg then, enzymolysis 8h opens in the enzymolysis process and stirs, and stirring velocity is controlled at 50 rev/mins;
(5) filtering separation: the enzyme processing is used the plate-and-frame filter press press filtration after finishing, and removes filter residue;
(6) Acid precipitation: in filtrate, add 6mol/L HCl, regulate pH to 2, and leave standstill 6h, make its precipitation;
(7) washing: the precipitation that step (6) is obtained adds water washing to pH5, and removes washings with the whizzer separation;
(8) neutralization: the precipitation that step (7) is obtained adds Na
2CO
3Be neutralized to pH8, be diluted to 500L, and fully stir,, get the monascorubin aqueous solution to dissolving fully;
(9) drying: the monascorubin aqueous solution spray-drier drying with step (8) obtains gets water-soluble monascus pigment powder 20～25kg;
(10) pulverize: the water-soluble monascus pigment powder that step (9) obtains is pulverized with pulverizing mill, made it meet the product granularity requirement.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101773244B (en) * | 2009-01-13 | 2012-07-04 | 陈小林 | Method for preparing water-soluble functional red yeast rice and product thereof |
| CN101831469B (en) * | 2010-05-31 | 2012-05-30 | 福建农林大学 | Method for preparing gardenia red pigment by using immobilized enzyme |
| CN103013165B (en) * | 2012-12-18 | 2014-05-28 | 山东中惠食品有限公司 | Method for preparing monascus red pigment by utilizing immobilized enzymes |
| CN103013166B (en) * | 2012-12-18 | 2014-07-30 | 山东中惠食品有限公司 | Method for preparing high-quality low-citrinin water-soluble monascus red pigment |
| CN103113755A (en) * | 2013-03-01 | 2013-05-22 | 江门科隆生物技术股份有限公司 | Extracting method for pigment in residues of monascus mycelium |
| CN104448919B (en) * | 2014-12-26 | 2016-06-01 | 天津商业大学 | The purification process of the pigment of hydroxyl |
| CN109234318B (en) * | 2018-09-25 | 2021-07-13 | 中南林业科技大学 | Method for improving monascus extracellular pigment |
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Non-Patent Citations (2)
| Title |
|---|
| 红曲色素提取的工艺条件及稳定性研究. 李浩然，杜竹玮，伍军.北京农学院学报，第19卷第1期. 2004 |
| 红曲色素提取的工艺条件及稳定性研究. 李浩然，杜竹玮，伍军.北京农学院学报，第19卷第1期. 2004 * |
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