CN102138663B - Method for processing fermented type nutritional fruit and vegetable powder rich in vitamin - Google Patents
Method for processing fermented type nutritional fruit and vegetable powder rich in vitamin Download PDFInfo
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Abstract
The invention provides a method for processing fermented type nutritional fruit and vegetable powder rich in vitamin. One or more yeast is utilized; fruit and vegetable juice squeezed from oranges, strawberries, tomatoes, apples, watermelons and grapes serve as basic materials; a special fruit and vegetable juice processing complex enzyme technique and a fruit and vegetable juice mixing process are combined; ventilated aerobic fermentation is performed for 40 to 60 hours; natural fruit and vegetable powder is processed by a spray drying technique; and the yield of the fruit and vegetable powder can reach 8 to 20 percent. Compared with the traditional fruit and vegetable powder, the fermented type nutritional fruit and vegetable powder is a pure natural green product, wherein after the fruit and vegetable powder is processed by procedures of enzyme treatment, fermentation and the like, the nutritional ingredients of vitamin, pectic oligosaccharide, amino acid and the like are greatly increased; dispersibility and water solubility are high; water content is less than or equal to 8 percent; and the fruit and vegetable powder is suitable to be preserved for a long time. A composite nutritional product can be produced by mixing the fruit and vegetable powder serving as the basic material and is widely applied in the fields of beverage, fast food, health-care products and the like. By the method, comprehensive economic benefit of fruit and vegetable industry can be increased on the whole, waste of resources is reduced and environment is protected. The method is a novel fruit and vegetable processing method.
Description
(1) technical field
The present invention relates to a kind of processing method that is rich in the fermented type nutrient fruit and vegetable powder of vitamin.
(2) background technology
At present China's fruit and vegetable food production ranks first in the world, still has sizable gap but compare with advanced country in the world.Present stage China's fruits and vegetables level of resources utilization is lower, adopts rear loss late height, and the fruit proportion of goods damageds reach 30%, and vegetables reach 40%～50%, and developed country's fruits and vegetables proportion of goods damageds are less than 7%.The aspect technological gaps such as particularly storing in fruits and vegetables postpartum, fresh-keeping, deep processing are larger, 10% of the not enough national total amount of overall processing capacity, and average out to 30% in the world, Germany even up to 75%.The fruits and vegetables high yield is not had a good harvest, and good harvest is not rised in value, and has become the bottleneck of restriction China fruits and vegetables industry fast development.
At present edible fruit mainly take except eating raw as main, follows traditional processing method simultaneously always, and such as canned, preserved fruit, fruit juice etc., these products have been difficult to satisfy the new demand that the consumer proposes food.Added value of product is lower, high value-added product then still less, therefore, the high nutrient fruit and vegetable product of added value of being badly in need of development of new comes the demand of satisfying the market.The fruits and vegetables powder is fruits and vegetables deep processing and a fine approach that improves the fruits and vegetables added value.Not only contain abundant vitamin and mineral matter in the fruits and vegetables powder, also contain dietary fiber, amino acid and organic acid etc.Traditional processing technology generally is directly Juice to be made after processing through making beating, high temperature drying.The fermented type fruits and vegetables powder that the present invention makes is compared with traditional fruits and vegetables powder, behind complex enzyme processing, microbial fermentation vitamin, pectin oligosaccharide, amino acid content significantly increases, and is a kind of fully natural green product.Carry out compositely take the fruits and vegetables powder as base stock, can produce composite nutritional products, be widely used in the fields such as beverage, instant food and health products.Increase on the whole the overall economic efficiency of fruits and vegetables industry, reduce the wasting of resources, protection of the environment, be the sustainable development generation deep effect of China's fruits and vegetables industry.
(3) summary of the invention
The object of the invention provides a kind of with overproduction or the inferior fruits and vegetables of low value, such as citrus, strawberry, tomato, apple, watermelon, grape, through the production technology of the eutrophy fruits and vegetables powder that complex enzyme is processed, microbial fermentation forms.By adopting the new and high technologies such as enzyme engineering technology, fermentation and metabolic engineering technology, modern low temperature drying technology, the content of vitamin, pectin oligosaccharide, amino acid etc. in the Effective Raise fruits and vegetables powder, improve quality and the recovery rate of product, reduce production costs, can develop suitable marketing, having the medicinal of certain physiological function and edible natural vitamin fruit and vegetable food, is the desirable feedstock as food, medicine and health products.
The technical solution used in the present invention is:
A kind of processing method that is rich in the fermented type nutrient fruit and vegetable powder of vitamin, described method comprises:
(1) pre-treatment: get fruit and vegetable materials, clean, remove the peel, pull an oar, obtain the fruits and vegetables slurries; Described fruits and vegetables are one of following: citrus, apple, grape, strawberry, tomato, watermelon; Concrete grammar can be as follows: the water that adds 10～20 times of quality after the fruits and vegetables peeling is processed front cleaning, removes the dirt of fruit and vegetable surfaces, then with beater garden stuff processing is become thick fruits and vegetables slurries.
(2) the phase I enzyme is processed: add cellulase and pectase complex enzyme powder in the fruits and vegetables slurries, adjust pH to 3.5～5.0 (citric acid or NaOH), at 40～45 ℃ of lower stir process 1～4h; Described cellulase consumption is 30～50U/g pulp liquid, and the pectase consumption is 15～40U/g pulp liquid; Usually complex enzyme opaque amount consumption is 0.06%～0.16% of fruits and vegetables slurries quality, its cellulase (30000～40000U/g) and pectase (30000～50000U/g) mass ratios are 2～3: 1; Need in the processing procedure constantly to stir, complete to accelerate enzymatic reaction;
(3) allotment: the fruits and vegetables slurries filter cleaner after step (2) is processed, get Juice, adding quality in the Juice is the nitrogenous source of Juice quality 1.5%～2.5%, and interpolation sucrose makes the soluble solid content of Juice reach 11Brix～19Brix, transfer pH to 4.5～5.0,111～121 ℃ sterilization 15～20min with citric acid or NaOH; Described nitrogenous source is yeast extract;
(4) fermentation: in deployed Juice, access saccharomycete, at 28～32 ℃, 120～350r/min bottom fermentation, 40～60h; Described saccharomycete is one of following or wherein two or more mixing: but salt tolerant flavouring yeast, Lang Bi Candida, KShi yeast, Shanghai are made yeast, grape wine high activity dried yeast and the super highly active dry yeast of 2.089 fruit wine; Zymotic fluid is concentrated into 15%～40% of original volume, gets concentrate; Inoculum concentration routinely inoculum concentration gets final product, and those of ordinary skills can determine that according to general knowledge with when enlarging the seed liquor inoculation after cultivating, seed liquor is 5～10% of Juice volume usually;
The preservation of barms and expansion cultural method:
Slant preservation culture medium (100mL): peptone 1g, yeast extract 1g, NaCl 0.4g, glucose 1g, agar 2g, pH 6.5.30 ℃ of constant temperature culture 2～3 days, the preservation temperature is 4 ℃.
First order seed culture medium (100mL): glucose 10g, peptone 1g, yeast extract 1g, MgSO
47H
2O 0.02g, KH
2PO
40.02g pH 5.0.30 ℃ of cultivation temperature, shaking speed 200r/min, incubation time 18h.
Secondary seed tank culture medium (100mL): glucose 10g, peptone 1g, yeast extract 1g, MgSO
4H
2O 0.02g, KH
2PO
40.02g pH 5.0.30 ℃ of cultivation temperature, mixing speed 200r/min, incubation time 24h.
(5) post processing: the concentrate spray-drying obtains described fermented type nutrient fruit and vegetable powder.
When the raw material for preparing described fermented type nutrient fruit and vegetable powder is citrus, described method also includes second stage enzyme treatment step between step (4) and step (5): concentrate pH is transferred to 4.1, add quality and be concentrate 0.3%～0.4% naringinase (10000～20000U/g), under 40 ℃ of constant temperature, process 1.5～2.5h.
Preferably; described naringinase is obtained through fermentation by aspergillus niger CCTCC NO:M 206047 (aspergillus niger WZ001 (Aspergillus niger WZ001)), and this bacterial strain is being protected as new bacterial strain in first to file CN200610051963.X.
The debitterize enzyme fermentation both can adopt solid-state shallow tray fermentation also can adopt the deep liquid aerobic fermentation.
Solid-state shallow tray fermentation: wheat bran 100g; K
2HPO
40.3g; Bean dregs (weight in wet base, dry weight is about 12%) 60g; Beancake powder 8g; Orange meal 3.5g, water 200mL, pH nature.Condition of culture: inoculum concentration is 10%～15%, and 32 ℃ of cultivation temperature are turned over song behind the cultivation 30h, incubation time 3-4 days altogether.Oven dry, dry place's preservation is for subsequent use after the sealing, naringinase enzyme activity 10000～20000U/g.。
Liquid ventilating fermentation (liquid amount by the 100L fermentation tank is 70L): bean dregs 7kg (weight in wet base, dry weight is about 12%); Beancake powder 1.75kg; Corn flour 0.7kg; Orange meal 0.7kg; K
2HPO
40.21kg; CaCl
270g; MgSO
47H
2O 70g; Defoamer (edible soybean oil) 50mL, pH 6.5.Condition of culture: cultivation temperature 30-32 ℃, incubation time 90～100h.Speed of agitator: front 20h is 150r/min, and DO (dissolved oxygen) is being got final product more than 30%; Control in real time through row progressively amplifying stirring frequency according to the dissolved oxygen reading behind the 20h.DO value minimum can not be lower than 10%, and is dry behind the filtering fermentation liquor, obtains the debitterize enzyme, naringinase enzyme activity 10000～20000U/g.
Described spray-drying condition is as follows: 55～60 ℃ of input concentration 30%～40%, temperature, 180～200 ℃ of inlet temperatures, 90～100 ℃ of outlet temperatures, air intake air pressure 0.25～0.3MPa, centrifugal turntable rotating speed 15000～20000r/min.
Beneficial effect of the present invention is mainly reflected in: according to the inventive method, make fruits and vegetables powder and traditional fruits and vegetables powder relatively, it is a kind of fully natural green product, the nutritional labelings such as its vitamin, pectin oligosaccharide, amino acid significantly increase after the processing of the operations such as enzyme processing, fermentation, wherein, content beta-carotene is 0.001%～0.05%, B family vitamin (VB
1, VB
2, nicotinic acid, VB
6Deng) in nicotinic acid, content is 0.2%～1.5%, pectin oligosaccharide content is 1%～10%; Dispersed and water-soluble all better in addition, moisture≤8% is more suitable for long-term preservation; Carry out compositely take this fruits and vegetables powder as base stock, can produce composite nutritional products, be widely used in the fields such as beverage, instant food, health products; Can increase on the whole the overall economic efficiency of fruits and vegetables industry, reduce the wasting of resources, protection of the environment, be the sustainable development generation deep effect of China's fruits and vegetables industry.
(4) specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
The preservation of barms is cultivated with expansion:
The slant preservation culture medium: peptone 1g, yeast extract 1g, NaCl 0.4g, glucose 1g, agar 2g, water 100mL, pH 6.5, high-temperature sterilization, cooling bevel.The access bacterial classification, 30 ℃ of constant temperature culture 2～3 days, the preservation temperature is 4 ℃.
First order seed culture medium: glucose 10g, peptone 1g, yeast extract 1g, MgSO
47H
2O0.02g, KH
2PO
40.02g, water 100mL, pH 5.0.Inoculation one ring slant strains, 30 ℃ of cultivation temperature, shaking speed 200r/min, incubation time 18h gets primary seed solution.
Secondary seed tank culture medium: glucose 10g, peptone 1g, yeast extract 1g, MgSO
4H
2O 0.02g, KH
2PO
40.02g, water 100mL, pH 5.0.By 5% volume ratio access primary seed solution, 30 ℃ of cultivation temperature, mixing speed 200r/min, incubation time 24h.
Salt tolerant flavouring yeast (Jining Yu Yuan bio tech ltd), but bright must Candida (DSMZ of Microbe Inst., Chinese Academy of Sciences, preservation center numbering: As2.1182), KShi yeast (the Shanghai enlightening is sent out and brewageed biological products Co., Ltd), the yeast (the Shanghai enlightening is sent out and brewageed biological products Co., Ltd) of 2.089 fruit wine is made in Shanghai, grape wine high activity dried yeast (Angel Yeast Co.,Ltd) and super highly active dry yeast (Angel Yeast Co.,Ltd) enlarge respectively cultivation according to the method described above, and it is for subsequent use to obtain respectively seed liquor.
The fermentation of debitterize enzyme:
After 5 days, inoculation one ring is cultivated 36h to black-koji mould WZ001 (CCTCC NO:M 206047) bacterial classification to liquid seed culture medium (wheat bran 4g, beancake powder 3g, bean dregs 8g, water 100mL), obtain seed liquor through the activation of Cha Shi culture medium.
Solid-state fermentation culture medium is wheat bran 100g, bean dregs 10g, beancake powder 20g, the water that adds 100ml, be sub-packed in the triangular flask of 500ml, charging thickness is 2.5～3.0cm, 0.1Mpa steam pressure sterilization 30min, after the cooling every bottle with volume ratio 2% access aspergillus niger seed liquor, in 32 ℃ of lower cultivations 5～7 days, filter to such an extent that debitterize enzyme liquid drying is for subsequent use by 1: 10 flooding after the culture oven dry, detect naringinase enzyme activity 10000～20000U/g in the enzyme powder.
Embodiment 2:
1. raw material is prepared: the Pon mandarin orange that pluck regional November take the Quzhou takes by weighing raw material 150kg as raw material after the selected classification, and directly full pulp making beating gets thick citrus pulp 118.5kg after the peeling, and it is for subsequent use to take out 50kg.
2. the thick citrus pulp of phase I enzyme treatment process: 50kg constantly stirs, and with NaOH pH is transferred to 4.2, and temperature rises to 42 ℃.Select that selectivity is strong, stability and good food-grade acid pectase and the cellulase of hydrolysis result be hybridly prepared into complex enzyme formulation, the mass ratio of cellulase and pectase is 3: 1.Add altogether 60g of complex enzyme formulation in thick citrus pulp, enzyme processing time is 90min.Acid pectase: enzyme 30000u/g alive, available from Wuxi snow plum preparation Science and Technology Ltd.; Cellulase: enzyme 40000u/g alive, available from the grand mcroorganism Engineering Co., Ltd in Shandong.
With the thick citrus pulp behind the complex enzyme zymohydrolysis through plate and frame filter press press filtration (filter cloth 120 orders), remove leather bag in the thick citrus pulp and the broken residues such as seed, get orange juice 40.4kg, crushing juice rate is 80.8%.
Contrast test: other gets not enzyme-added comparing of the thick citrus pulp of 50kg, directly with thick citrus pulp squeezing, gets 34.6kg, and crushing juice rate is 69.2%.
This shows that adding the rear crushing juice rate of complex enzyme processing has increased by 11.7%.The crushing juice rate by but the catalytic reaction Effective Raise fruit of pectase and cellulase is processed improves the fruit juice quality and yield, and is simple for process.Pectase can soften the pectic substance in the pulp organization, makes insoluble pectin substance be decomposed into the pectin oligomer of solubility, to reduce the viscosity of pulp, can obviously improve the fruit juice productive rate.Cellulase synergistic destroys the fibre structure in the fruits and vegetables, is the utilizable reductive monosaccharide of microorganism with cellulose degradation.The transparency of fruit juice and clarity are able to obvious raising, have also increased the content of soluble solid.
4. the allotment of orange juice and fermentation:
(1) allotment of orange juice: press mass fraction 1.8% and in the 40.4kg orange juice, add nitrogenous source yeast extract 0.73kg; The pol that detects orange juice with hand-held saccharometer is 13.5Brix, adds 2.70kg sucrose in the 40.4kg orange juice to make the orange juice pol reach 18Brix; With NaOH orange juice pH is transferred to 5.0.121 ℃ of sterilization 15min are cooled to room temperature.
(2) in the autocontrol mechanical agitating fermentation tank of 65L, the salt tolerant flavouring yeast starter liquid for preparing is moved into the deployed orange juice of 41L by the inoculum concentration of 10% (v/v), making liquid amount is 45L.Control tank pressure 0.03Mpa passes into air pressure 0.12Mpa, air velocity 25NL/min, and 30 ℃ of temperature, mixing speed 300r/min, fermentation time are 50h.
5. take citrus fruit as raw material, contain a large amount of bitter substances in the zymotic fluid, the main source of bitter taste is aurantiin, therefore, removes aurantiin the fruit juice bitter taste is alleviated.The used enzyme of enzyme process debitterize mainly is naringinase, and it is comprised of alpha-L-Rhamnosidase and β-D-Glucose glycosides enzyme.Alpha-L-Rhamnosidase can be hydrolyzed into prunin and rhamnose with the aurantiin of flavanone glycoside compounds in the citrus fruit juice, and the bitter taste of prunin is about 1/3 of aurantiin, so bitter taste alleviates to some extent; Prunin can generate naringenin and the glucose without bitter taste under β-D-Glucose glycosides enzyme continuation effect.It is that naringinase is processed that zymotic fluid is carried out second step debitterize enzyme, and concrete grammar is:
Ratio in 20% is concentrated to 9kg with zymotic fluid, detects the about 2.15g/L of naringin content in the concentrate.The enzyme treatment conditions are:
(1) 40 ℃ of constant temperature of concentrate, pH transfers to 4.1.
(2) the enzyme addition is 0.3% of concentrated after fermentation liquid quality, needs to add embodiment 1 debitterize enzyme (naringinase enzyme activity 10000U/g) enzyme powder 27g, and the debitterize time is 2h.
6. the concentrate after the debitterize enzyme is processed is carried out spray-drying processing, input concentration is about 40%, 60 ℃ of feeding temperatures, and 180～200 ℃ of inlet temperatures, 90～100 ℃ of outlet temperatures, air intake air pressure 0.25～0.3MPa, the centrifugal turntable rotating speed remains on 18000r/min.
7. obtain fermented type eutrophy citrus grain weight 3.61kg after the drying, recovery rate is: 8.94% (accounting for orange juice proportion after filtering), moisture≤4%.Various vitamin contents such as following table 1 in the fermented type eutrophy citrus powder.After cooling sealing places 4 ℃ of dry place's preservations.
Table 1: several vitamins content (100g) in the fermented type eutrophy citrus powder
Embodiment 3:
1. raw material is prepared: take the regional Pon mandarin orange in the Quzhou of embodiment 2 as raw material, choose 150kg, full pulp making beating gets thick citrus pulp 116.0kg after the peeling, and 50kg is for subsequent use in taking-up.
2. phase I enzyme treatment process: the thick citrus pulp of 50kg according to embodiment 2 step regulatory enzyme reaction conditions, is added complex enzyme formulation, and the ratio of cellulase and pectase is 2: 1.Add complex enzyme formulation 70g in thick citrus pulp, enzyme processing time is 90min.Acid pectase: enzyme 30000u/g alive, available from Wuxi snow plum preparation Science and Technology Ltd.; Cellulase: enzyme 40000u/g alive, available from the grand mcroorganism Engineering Co., Ltd in Shandong.
3. the filter progress according to embodiment 2 gets orange juice 42.1kg, and crushing juice rate is 84.2%.Contrast test: other gets not enzyme-added comparing of the thick citrus pulp of 50kg, directly with thick citrus pulp squeezing, gets 34.6kg, and crushing juice rate is 69.2%.This shows that adding the rear crushing juice rate of complex enzyme processing has increased by 15.0%.
4. the allotment of orange juice and fermentation:
(1) allotment of orange juice: press mass fraction 2.0% and add nitrogenous source yeast extract 0.84kg in the 42.1kg orange juice, the pol that detects orange juice with hand-held saccharometer is 13.8Brix, adds 2.67kg sucrose in the 42.1kg orange juice to make the orange juice pol reach 18Brix; With NaOH orange juice pH is transferred to 5.0.121 ℃ of sterilization 15min are cooled to room temperature.
(2) in the autocontrol mechanical agitating fermentation tank of 65L, but brightly must the Candida seed liquor move into the deployed orange juice of 42L by the inoculum concentration of 10% (v/v) with what prepare, making liquid amount is 46L.Control tank pressure 0.03Mpa passes into air pressure 0.12Mpa, air velocity 25NL/min, and 30 ℃ of temperature, mixing speed 300r/min, fermentation time are 50h.
5. zymotic fluid is carried out second step debitterize enzyme and process, the ratio in 20% is concentrated to 9.2kg with zymotic fluid, detects the about 2.22g/L of naringin content in the concentrate.The enzyme treatment conditions: 40 ℃ of constant temperature, pH are 4.1.Debitterize enzyme (naringinase enzyme activity 10000U/g) enzyme powder addition adds 27g approximately for 0.3% of concentrated after fermentation liquid quality, and the debitterize time is 2h.
6. carry out spray drying treatment according to 2 pairs of concentrates of embodiment, obtain dry after fermentation type eutrophy citrus grain weight 3.80kg, recovery rate is: 9.05% (accounting for orange juice proportion after filtering), moisture≤4%.Various vitamin contents such as following table 2 in the fermented type eutrophy citrus powder.After cooling sealing places 4 ℃ of dry place's preservations.
Table 2: several vitamins content (100g) in the fermented type eutrophy citrus powder
Embodiment 4:
1. raw material is prepared: take the regional Pon mandarin orange in the Quzhou of embodiment 2 as raw material, choose 150kg, full pulp making beating gets thick citrus pulp 108.8kg after the peeling, and 50kg is for subsequent use in taking-up.
2. phase I enzyme treatment process: the thick citrus pulp of 50kg according to embodiment 2 step regulatory enzyme reaction conditions, is added complex enzyme formulation, and the ratio of cellulase and pectase is 2: 1.Add complex enzyme formulation 70g in thick citrus pulp, enzyme processing time is 90min.Acid pectase: enzyme 30000u/g alive, available from Wuxi snow plum preparation Science and Technology Ltd.; Cellulase: enzyme 40000u/g alive, available from the grand mcroorganism Engineering Co., Ltd in Shandong.
3. the filter progress according to embodiment 2 gets orange juice 41.4kg, and crushing juice rate is 82.8%.Contrast test: other gets not enzyme-added comparing of the thick citrus pulp of 50kg, directly with thick citrus pulp squeezing, gets 34.6kg, and crushing juice rate is 69.2%.This shows that adding the rear crushing juice rate of complex enzyme processing has increased by 13.6%.
4. the allotment of orange juice and fermentation:
(1) allotment of orange juice: press mass fraction 1.7% and in the 41.4kg orange juice, add nitrogenous source yeast extract 0.70kg; The pol that detects orange juice with hand-held saccharometer is 13.5Brix, adds 2.82kg sucrose in the 41.4kg orange juice to make the orange juice pol reach 19Brix; With NaOH orange juice pH is transferred to 5.0.121 ℃ of sterilization 15min are cooled to room temperature.
(2) in the autocontrol mechanical agitating fermentation tank of 65L, the KShi yeast starter liquid for preparing is moved into the deployed orange juice of 41L by the inoculum concentration of 7% (v/v), making liquid amount is 45L.Control tank pressure 0.03Mpa passes into air pressure 0.12Mpa, air velocity 25NL/min, and 30 ℃ of temperature, mixing speed 300r/min, fermentation time are 52h.
5. zymotic fluid is carried out second step debitterize enzyme and process, the ratio in 20% is concentrated to 9.0kg with zymotic fluid, detects the about 2.14g/L of naringin content in the concentrate.The enzyme treatment conditions: 40 ℃ of constant temperature, pH are 4.1.Debitterize enzyme (naringinase enzyme activity 10000U/g) enzyme powder addition adds 27g approximately for 0.3% of concentrated after fermentation liquid quality, and the debitterize time is 2h.
6. carry out spray drying treatment according to 2 pairs of concentrates of embodiment, obtain dry after fermentation type eutrophy citrus grain weight 3.72kg, recovery rate is: 8.98% (accounting for orange juice proportion after filtering), moisture≤4%.Various vitamin contents such as following table 3 in the fermented type eutrophy citrus powder.After cooling sealing places 4 ℃ of dry place's preservations.
Table 3: several vitamins content (100g) in the fermented type eutrophy citrus powder
Embodiment 5:
1. raw material is prepared: taking by weighing clean, sorting after ripening degree is the fresh tomato 110kg 9.0 or more, full fruit making beating, and the thick strained tomatoes of taking-up 50kg is for subsequent use.
2. the thick strained tomatoes of phase I enzyme treatment process: 50kg constantly stirs, and with citric acid pH is transferred to 4.2, and temperature rises to 42 ℃.Select that selectivity is strong, stability and good food-grade acid pectase and the cellulase of hydrolysis result be hybridly prepared into complex enzyme formulation, the ratio of cellulase and pectase is 3: 1.Add complex enzyme formulation 40g in thick strained tomatoes, enzyme processing time is 60min.Acid pectase: enzyme activity 30000u/g, available from Wuxi snow plum preparation Science and Technology Ltd.; Cellulase: enzyme activity 40000u/g, available from the grand mcroorganism Engineering Co., Ltd in Shandong.
With the thick strained tomatoes behind the complex enzyme zymohydrolysis through plate and frame filter press press filtration (filter cloth 120 orders), remove leather bag in the thick strained tomatoes and the broken residues such as seed, get tomato juice 43.7kg, crushing juice rate is 87.4%.
Contrast test: other gets not enzyme-added comparing of the thick strained tomatoes of 50kg, directly with thick strained tomatoes squeezing, gets 40.9kg, and crushing juice rate is 81.8%.This shows that adding the rear crushing juice rate of complex enzyme processing has increased by 5.6%.
4. the allotment of tomato juice and fermentation:
(1) allotment of tomato juice: press mass fraction 1.8% and add nitrogenous source yeast extract 0.78kg in the 43.7kg orange juice, the pol that detects tomato juice with hand-held saccharometer is 4.5Brix, adds 3.8kg sucrose in the tomato juice to make pol reach 12.5Brix; With NaOH orange juice pH is transferred to 5.0.121 ℃ of sterilization 15min are cooled to room temperature.
(2) in the autocontrol mechanical agitating fermentation tank of 65L, the super brewer yeast seed liquor for preparing is moved into the deployed tomato juice of 44L by the inoculum concentration of 10% (v/v), making liquid amount is 48L.Control tank pressure 0.03Mpa passes into air pressure 0.13Mpa, air velocity 27NL/min, and 30 ℃ of temperature, mixing speed 330r/min, fermentation time are 42h.
5. in 30% ratio zymotic fluid is concentrated to 14.4kg, concentrate is carried out spray-drying processing, input concentration about 40%, 60 ℃ of feeding temperatures, 180～200 ℃ of inlet temperatures, 90～100 ℃ of outlet temperatures, air intake air pressure 0.25～0.3MPa, the centrifugal turntable rotating speed remains on 20000r/min.
6. obtain fermented type eutrophy tomato grain weight 5.89kg after the drying, recovery rate is: 13.47% (accounting for tomato juice proportion after filtering), moisture≤5%.Various vitamin contents such as following table 4 in the fermented type eutrophy tomato meal.After cooling sealing places 4 ℃ of dry place's preservations.
Table 4: several vitamins content (100g) in the fermented type eutrophy tomato meal
Claims (3)
1. processing method that is rich in the fermented type nutrient fruit and vegetable powder of vitamin, described method comprises:
(1) pre-treatment: get fruit and vegetable materials, clean, remove the peel, pull an oar, obtain the fruits and vegetables slurries; Described fruits and vegetables are one of following: citrus, tomato;
(2) the phase I enzyme is processed: add cellulase and pectase complex enzyme powder in the fruits and vegetables slurries, adjust pH to 3.5～5.0, at 40～45 ℃ of lower stir process 1～4h; Described cellulase consumption is 30～50U/g pulp liquid, and the pectase consumption is 15～40U/g pulp liquid;
(3) allotment: the fruits and vegetables slurries filter cleaner after step (2) is processed, get Juice, adding quality in the Juice is the nitrogenous source of Juice quality 1.5%～2.5%, and interpolation sucrose makes the soluble solid content of Juice reach 11Brix～19Brix, transfer pH to 4.5～5.0,111～121 ℃ sterilization 15～20min with citric acid or NaOH; Described nitrogenous source is yeast extract;
(4) fermentation: in deployed Juice, access saccharomycete, at 28～32 ℃, 120～350r/min, ventilation 0.5～1.0vvm bottom fermentation 40～60h; Described saccharomycete is one of following or wherein two or more mixing: salt tolerant flavouring yeast, KShi yeast, the Shanghai enlightening that Jining Yu Yuan bio tech ltd produces sent out the super highly active dry yeast that the yeast brewageing Shanghai that biological products Co., Ltd produces and make 2.089 fruit wine, grape wine high activity dried yeast that Angel Yeast Co.,Ltd produces and Angel Yeast Co.,Ltd produce; Zymotic fluid is concentrated into 15%～40% of original volume, gets concentrate;
(5) post processing: the concentrate spray-drying obtains described fermented type nutrient fruit and vegetable powder; When the raw material for preparing described fermented type nutrient fruit and vegetable powder is citrus, described method also includes second stage enzyme treatment step between step (4) and step (5): concentrate pH is transferred to 4.1, add the naringinase of 10～100U/g concentrate, under 40 ℃ of constant temperature, process 1.5～6.5h.
2. the method for claim 1 is characterized in that described naringinase is obtained through fermentation by aspergillus niger CCTCC NO:M 206047.
3. the method for claim 1, it is characterized in that described spray-drying condition is as follows: 55～60 ℃ of input concentration 30%～40%, temperature, 180～200 ℃ of inlet temperatures, 90～100 ℃ of outlet temperatures, air intake air pressure 0.25～0.3MPa, centrifugal turntable rotating speed 15000～20000r/min.
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|---|---|---|---|
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| CN102754782A (en) * | 2012-06-26 | 2012-10-31 | 浙江工业大学 | Preparation method of fermentation type fruit and vegetable powder rich in gamma-aminobutyric acid and lactobacillus plantarum |
| CN103462034A (en) * | 2013-08-13 | 2013-12-25 | 当涂县瑞龙果树种植专业合作社 | Winy cereal fruit-vegetable powder and its preparation method |
| CN104605277A (en) * | 2013-11-05 | 2015-05-13 | 熊涛 | Functional probiotic fermented fruit and vegetable powder preparation method |
| CN103798738B (en) * | 2014-03-09 | 2015-04-15 | 浙江绿晶香精有限公司 | Preparation method of fermented type pure natural strawberry essence base material |
| CN103947757A (en) * | 2014-05-22 | 2014-07-30 | 于法周 | Processing method of fig instant powder |
| CN104664405A (en) * | 2014-09-04 | 2015-06-03 | 杭州隽艺生物技术有限公司 | Fat-reducing and weight-losing fruit enzyme powder and preparation method thereof |
| CN106307534A (en) * | 2016-08-25 | 2017-01-11 | 彭常安 | Fabrication method for instant crystal for fermentation of der bockdorn and chrysanthemum nankingense |
| CN108464465B (en) * | 2018-03-16 | 2021-08-31 | 山东省农业科学院农产品研究所 | Preparation method of fermented grape whole fruit powder |
| CN109198429A (en) * | 2018-08-03 | 2019-01-15 | 齐鲁工业大学 | One kind i.e. punching fermentation five cereals fruit vegetable powder and preparation method thereof |
| CN111449181A (en) * | 2020-04-09 | 2020-07-28 | 广东省农业科学院蚕业与农产品加工研究所 | Method for preparing fermentation type composite fruit and vegetable beverage through ultrahigh pressure auxiliary enzyme treatment |
| CN113785970A (en) * | 2021-09-01 | 2021-12-14 | 厦门益力康生物科技有限公司 | Combined enzyme |
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| CN101314751A (en) * | 2007-06-01 | 2008-12-03 | 海南省工业研究所 | Brewing method for a fruit wine |
| CN101899372A (en) * | 2010-02-08 | 2010-12-01 | 广东十二岭酒业有限公司 | Production method of sugar orange fruit wine |
| CN101942378A (en) * | 2010-09-26 | 2011-01-12 | 杜刚 | Method for brewing cherry fruit wine |
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| CN101314751A (en) * | 2007-06-01 | 2008-12-03 | 海南省工业研究所 | Brewing method for a fruit wine |
| CN101899372A (en) * | 2010-02-08 | 2010-12-01 | 广东十二岭酒业有限公司 | Production method of sugar orange fruit wine |
| CN101942378A (en) * | 2010-09-26 | 2011-01-12 | 杜刚 | Method for brewing cherry fruit wine |
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