CN102517380A - Method for screening microorganism strains for efficient degradation of tea seedcake meal - Google Patents
Method for screening microorganism strains for efficient degradation of tea seedcake meal Download PDFInfo
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Abstract
Provided is a method for screening microorganism strains for efficient degradation of tea seedcake meal. The method enables the microorganism strains to be obtained by sequentially performing a primary screening of a high-concentration tea seedcake meal culture medium, a secondary screening of a casein culture medium, a third-time screening of a carboxymethyl cellulose culture medium and bottle shaking fermentation secondary screening. The strains have tolerance to high-concentration tea saponin of the tea seedcake meal, high protease activity and performance of activity of high cellulose enzyme simultaneously. The strain-fermented tea seedcake meal obtained by the method has nutritional value higher than that the tea seedcake meal which is not fermented, protein content of crude protein of a product is 8%-12%, free amino acid content is over 7.5% and is improved by 40%, crude fiber content is 20%-25%, reducing sugar content is over 6.0% and is improved by 15%, saponin content is below 0.5%, and detoxification rate is over 95%. The method well solves the problems of toxicity of the tea seedcake meal and low utilization rate of the crude fiber and the crude protein in the prior art. A production process method of the multi-strain fermented tea seedcake meal can be simplified by utilizing the method to obtain the strains, and the method can obtain the microorganism strains which are simplified in process and good in degradation effects.
Description
Technical field
The present invention relates to the method for the biological bacterial strain of a cover screening efficient degradation tea waste, belong to the biological technical field that fermentation engineering, biomass resource are utilized again.
Background technology
Oil tea is China a kind of important oil crops in southern hills area, accounts for more than 80% of national woody edible oil materials crop.Tea seed cake is claimed the tea dregs of rice again, and another name tea bran, tea are withered, tea seed cake.Being the puce particle, is the remaining residue in Oleum camelliae fruit oil expression back.Also contain a large amount of starch, protein, crude fat, soluble sugar and tea saponin etc. in the grouts of tea seed after oil expression, again extraction and application.About 4,000,000 hectares of China's camellia oleifera lam area is that the oil tea kind at most in the world, the country the widest, that output is maximum distributes.According to statistics, China plucks the tea place area and is about 1,000,000 hectares, and China recent years is produced about 550,000 tons of oil tea tea seed per year, and oil tea tea seed oil length is 26% ~ 39%, and the machine of pressing presses oil yield 70% and calculates about 400,000 tons of the average annual output of cake of camellia oleifera seeds.China's camellia oleifera lam mainly is distributed in Hunan, Jiangxi, Guangxi San Sheng, and three province's oil tea tea seed output account for more than 80% of the whole nation.China's oil tea leached tea oil slag and tealeaves leached tea oil slag output are about 700,000 tons, this shows, China's potential tea dregs of rice capable of using resource is very abundant.The tea oil tea-cake dregs generally contains 7% crude fat, 14% ~ 15% crude protein, and 33% robust fibre, the carbohydrate more than 40%, tea saponin is about 12% ~ 18%, is the available tea seed of potential residue resource.Thereby the development and use leached tea oil slag has high economic benefit.At present; The tea seed mainly adopts traditional milling process milling process system oil, ANFs too high levels such as tea saponin, tannin in the tea dregs of rice of being produced, the unsuitable production that directly is used for animal-feed; Major part is used as pool-cleaning agent, fertilizer, fuel, is used for the extraction of tea saponin on a small quantity.Simultaneously, because of containing 10% ~ 15% hemolytic tea saponin in the tea waste, and the existence of tea saponin makes feed bitter and pungent, and hydrocoles such as fish are had very strong toxicity, therefore, has limited its application in fodder industry.But, removed the toxic substance tea saponin like the fruit tea dregs of rice, its nutritive value can match in excellence or beauty with rice bran; And after undergoing microbial fermentation; Can obtain higher tropina, not only can improve the utility value of the tea dregs of rice, and can make it to become a kind of feed resource preferably through the degradation bacteria fermentation
Also relatively weaker to the tea waste Research on degradation at present, representational research has: Xiao Yujuan etc. are material with the tea dregs of rice that extract behind the tea saponin, research
Neurospora.crassaThe robust fibre of (sturdy vein born of the same parents bacterium) degraded tea dregs of rice substratum; Li Junjun etc. obtain 6 strain bacterial classification cellulase producing bacterias through substratum cellulases such as Congo red substratum, CMC 99.5 substratum; Gem prosperous grade in field obtains can effectively the degrade bacterial strain T7 of tea seed cake with strong bacteriostatic action of a strain from occurring in nature screening.Patent KR 20110090215 disclose a kind of have the cleansing performance of guaranteeing good, rise soak,, humidity-holding effect good liquid detergent composition little to skin irritation; Comprising 5% ~ 40% AS tea saponin; 0.5 ~ 20% nonionogenic tenside or amphoterics; 0.01 ~ 10% lactic acid and saponin, these words of remaining tailwater quantity are obstructed.Saponin is extracted acquisition from green tea or Chinese honey locust.Patent WO2011113221 discloses a kind of washing composition of being made up of 70% ~ 80% Limonene, 2% ~ 10% tea saponin, 1% ~ 10% Yelkin TTS, 2% ~ 10% itral and 0.5% ~ 1% Hydrocerol A, and this washing composition is damage car, paint film and household appliance glass not.U.S. Pat 6007822 discloses a kind of animal feedstuff compsns that comprises Camellia plant triterpene saponin(e and in the human and animal, has had the raising immunologic function, strengthens antibiotic and antiviral activity, mutation, anti-oxidant and removing radical.Patent CN1148598 discloses a kind of method of extracting refined theasapogenin, obtains the solid tea saponin more than 98%.Report about the tea waste detoxification is more rarely seen; For example Mr. Huang Liangbai has described a kind of technology of tea waste detoxification in " the residual tea saponin Determination on content of detoxification tea dregs of rice method relatively " literary composition; With the lixiviate of the degreasing tea dregs of rice, solid-liquid separation, evaporation concentration; Spraying drying obtains feed slag and tea saponin respectively.This method essence is to extract tea saponin, and subsidiary property acquisition feed slag belongs to the physics detoxification.But these researchs solve one of them problem from a side of tea waste; The just research of tea waste detoxification aspect; The just research of degrade coarse fibers aspect; The research of the crude protein aspect of just degrading lacks tea saponin, robust fibre and crude protein research method in the systematicness degraded tea waste.
Summary of the invention
Technical problem to be solved by this invention is: to the deficiency of above-mentioned prior art; A kind of systems approach that obtains the microorganism strains of efficient degradation tea waste is provided; This method had both comprised the tea waste detoxification; Comprising the coarse-fibred degraded of tea waste again, also comprise the degraded of tea waste crude protein, is the method for tea saponin, robust fibre and crude protein in a kind of system degraded tea waste.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopted comprises following step:
⑴ high density tea saponin tolerance bacterial strain screening: compounding high concentration tea waste solid medium, wherein tea waste 10% ~ 15%, agar 1.5% ~ 2.0%; Water 85.0% ~ 90.0%, pH nature, 120 ~ 130 ℃ of sterilization 20 ~ 30 min; The flat board that falls is some, and each flat board supplies examination soil samples or water sample to obtain tea waste waste sample diluting liquid 0.1 mL by 10 times of dilution methods coatings, cultivates 3 ~ 5 d at 30 ℃ ~ 40 ℃; Bacterium colony is big, that rising trend is good bacterial strain switching inclined-plane; Obtain tea saponin tolerance bacterial strain, numbering, the screening that supplies further to obtain protein-high enzymic activity bacterial strain is used.
⑵ protein-high enzymic activity bacterial strain screening: preparation casein food grade solid medium, wherein casein food grade 1.0% ~ 2.0%, after the 0.1 mol/L Hydrogen chloride heating for dissolving; Glucose 2.0% ~ 4.0%, agar 1.5% ~ 2.0% is transferred pH to 5.0 ~ 7.0 with 0.1mol/L sodium hydroxide; All the other are water; Promptly be settled to the reservation volume with tap water, 120 ~ 130 ℃ of sterilization 20 ~ 30 min fall dull and stereotyped some.With getting tea saponin tolerance bacterial strain among the foregoing invention technical scheme ⑴; Point is inoculated in the casein substrate upper flat plate; Cultivate 2 ~ 3 d at 30 ℃ ~ 40 ℃, with hydrolysis loop diameter and colony diameter ratio bacterial strain big, that rising trend the is good aseptic inclined-plane of transferring once more, acquisition proteolytic enzyme high reactivity bacterial strain; Numbering supplies the further screening of screening high-cellulose enzymic activity bacterial strain to use.
⑶ high-cellulose enzymic activity bacterial strain screening: preparation CMC 99.5 solid medium, wherein the CMC 99.5 element 1.0% ~ 2.0%, after the tap water heating for dissolving; Peptone 0.3% ~ 0.5%; Agar 1.5% ~ 2.0%, natural pH is settled to the reservation volume with tap water; 121 ℃ of sterilization 25 ~ 30 min fall dull and stereotyped some.With getting proteolytic enzyme high reactivity bacterial strain among the foregoing invention technical scheme ⑵, point is inoculated in CMC 99.5 substratum upper flat plate, cultivates 2 ~ 3 d at 30 ℃ ~ 40 ℃; With 0.1% ~ 0.5% aseptic Congo red liquid topic dyeing 5 min; Use 2.0% ~ 5.0% aseptic sodium chloride solution, 5 min that decolour again, select hydrolysis loop diameter and colony diameter ratio bacterial strain big, that rising trend the is good aseptic inclined-plane of transferring once more, acquisition cellulase high reactivity bacterial strain is some; And numbering, 4 ℃ of refrigerator preservations, subsequent use.
⑷ shake flask fermentation degraded tea waste further sieves again: with getting tea saponin height endurability, protein-high enzymic activity and high-cellulose enzymic activity bacterial strain among the described foregoing invention technical scheme ⑶, be used for shake flask fermentation degraded tea waste and further sieve again.Wherein, substratum is got tea waste 2% ~ 5% by weight percentage, and weight in wet base is 95% ~ 98%; The pH nature, the some bottles of 100 mL place 250 mL triangular flasks; 120 ~ 130 ℃ of sterilization 20 ~ 30 min inoculate foregoing invention technical scheme ⑶ respectively and obtain bacterial strain, under 200 ~ 300 r/min conditions; Shaking table shaking culture 72 ~ 84 h; Measure tea saponin content, free aminoacid content, reducing sugar content, crude protein degradation rate, robust fibre degradation rate, proteinase activity, cellulase activity in the fermented liquid, select the bacterial strain that tea saponin content is low, free aminoacid content is high, reducing sugar content is high, the crude protein degradation rate is high, the robust fibre degradation rate is high, proteinase activity is high, cellulase activity is high, numbering; As the production bacterial classification of tea waste fermentation, 4 ℃ of refrigerator preservations, subsequent use.
⑸ tea waste fermentative degradation:
1. strain preparation: get tea waste 2% ~ 5%, wheat bran 1% ~ 2% by weight percentage; Weight in wet base is 93% ~ 97%, pH nature, 120 ~ 130 ℃ of sterilization 20 ~ 30 min; Inoculation foregoing invention technical scheme ⑷ obtains some bacterial strains; Under 200 ~ 300r/min condition, shaking table shaking culture 24 ~ 36h makes that cell concentration reaches 10 in this seed culture fluid
8~ 10
9Cfu/mL is as the production bacterial classification of tea waste fermentation;
2. tea waste solid fermentation degraded: get tea waste 40% ~ 50% by weight percentage, weight in wet base is that 50% ~ 60%, 120 ~ 130 ℃ of sterilization 30 ~ 40 min process tea waste solid fermentation substratum; Inoculating the foregoing invention technical scheme 1. obtains to produce bacterial classification and is inoculated in this tea waste solid fermentation substratum with 1% ~ 3% inoculation weight; Mix; Be piled into high 0.5 ~ 0.8 m, the ridge heap that length width is not limit covers straw screen or mat above; 45 ~ 55 ℃ of bottom fermentations 5 ~ 10 days, the solid tea waste that promptly gets detoxification after the drying and degraded;
3. tea waste liquid fermenting degraded: get tea waste 10% ~ 15% by weight percentage, weight in wet base is 85% ~ 90%, in 100 L ~ 1000 L fermentor tanks, 120 ~ 130 ℃ of sterilization 30 ~ 40 min process the tea waste liquid fermentation medium; Again 1. step is obtained to produce bacterial classification and be inoculated into 1% ~ 3% inoculation weight and be equipped with in this tea waste liquid fermentation tank, 32 ~ 37 ℃ of following ventilating fermentations 4 ~ 6 days, the tea waste hydrolyzed solution that promptly gets detoxification after concentrating and degraded.
Compared with prior art, the present invention has the following advantages:
⑴ in the microbe to screen process of the inventive method; Successively adopt three indexs; Be high tea saponin concentration tolerance, protein-high enzymic activity and high-cellulose enzymic activity, obtained strains has both degradable crude protein, degradable robust fibre again; Be low toxicity or nontoxic also with the tea waste detoxification, complete function;
⑵ be raw material with the not detoxification tea waste of the existing explained hereafter of China, adopts the inventive method to obtain the strain fermentation tea waste, and its value is higher than unleavened tea waste; Product crude protein protein contnt is 8% ~ 12%; Free aminoacid content reaches more than 7.5%, and crude fiber content is 20 ~ 25%, and reducing sugar content reaches more than 6.0%; Saponin content is lower than 0.5%, and virus elimination rate is higher than 95%.This method can solve the problem that tea waste toxicity, crude protein are thick and fibre content is high that prior art exists well.
⑶ the present invention is the utilization of the agricultural byproduct waste degree of depth, have that production technique is simple, the no three wastes, invest little, be easy to characteristics such as large-scale production.Utilizing this method to obtain bacterial strain and can simplify many bacterial strains, multi-strain fermentation tea waste producing and manufacturing technique, reduced the microbial fermentation tea waste and used the bacterial strain kind, is a kind of method that can obtain the microorganism strains of simplification technology, good degrading effect.
Embodiment:
Following examples are described further the present invention.
Embodiment 1: screening high density tea saponin tolerance bacterial strain, and compounding high concentration tea waste solid medium, wherein tea waste 10%, agar 2.0%; Water 88.0%, constant volume 100 mL, pH nature, 121 ℃ of sterilization 25 min; Fall dull and stereotyped 8, each flat board is pressed 10 times of dilution methods coatings and is supplied examination soil sample diluent 0.1 mL, and at 35 ℃ of cultivation 4d, the acquisition bacterium colony is big, rising trend is good, 7 strains of tea saponin tolerance bacterial strain; Be numbered lys2201, lys2202, lys2203, lys2204; Lys2205, lys2206, lys2207, and switching inclined-plane.
Embodiment 2: screening protein-high enzymic activity bacterial strain, and preparation casein food grade solid medium, wherein casein food grade 2.0%; 0.1 after the mol/L Hydrogen chloride heating for dissolving, glucose 3.0%, agar 2.0%; Transfer pH to 5.5 with 0.1 mol/L sodium hydroxide; Be settled to 100 mL with tap water, 121 ℃ of sterilization 25 min fall dull and stereotyped 8.With bacterial strain lys2201, lys2202, lys2203, lys2204, lys2205, lys2206; The lys2207 point is inoculated in the casein substrate upper flat plate; Cultivate 2.5 d at 33 ℃, with hydrolysis loop diameter and colony diameter ratio greatly, bacterial strain that rising trend the is good aseptic inclined-plane of transferring once more, wherein the hydrolysis loop diameter of bacterial strain lys2201, lys2203, lys2204, lys2205, lys2207 and colony diameter ratio are all greater than 5.0; And the switching inclined-plane, do further to sieve again subsequent use.
Embodiment 3: screening high-cellulose enzymic activity bacterial strain, and preparation CMC 99.5 solid medium, wherein CMC 99.5 2.0%, after the tap water heating for dissolving; Peptone 0.5%, agar 2.0%, natural pH; Be settled to 100 mL with tap water, 121 ℃ of sterilization 25 min fall dull and stereotyped 8.With bacterial strain lys2201, lys2202, lys2203, lys2204, lys2205, lys2206; The lys2207 point is inoculated in CMC 99.5 substratum upper flat plate, cultivates 3 d at 33 ℃, with 0.2% aseptic Congo red liquid 5 mL; Dull and stereotyped water 5 min dye; Again with 2.0% aseptic sodium chloride solution, 5 min that decolour, select hydrolysis loop diameter and colony diameter ratio greatly, bacterial strain that rising trend the is good aseptic inclined-plane of transferring once more, wherein the hydrolysis loop diameter of bacterial strain lys2201, lys2202, lys2203, lys2207 and colony diameter ratio are all greater than 4.0; And the switching inclined-plane, 4 ℃ of refrigerator preservations, subsequent use.
Embodiment 4: the tea waste shake flask fermentation sieves again, and preparing culture medium is got tea waste 4% by weight percentage, and weight in wet base is 94%; The pH nature, 7 bottles of 100 mL place the 250mL triangular flask; 121 ℃ of sterilization 25 min, difference inoculating strain lys2201, lys2202, lys2203, lys2204, lys2205, lys2206, lys2207; Under the 200 r/min conditions; Shaking table shaking culture 84 h, wherein bacterial strain lys2207 fermented liquid tea saponin content is 0.2%, free aminoacid content 7.6%, reducing sugar content 5.3%, proteinase activity and cellulase activity be the highest, therefore; Select the production bacterial classification of bacterial strain lys2207,4 ℃ of refrigerator preservations, subsequent use as the tea waste fermentation.
Embodiment 5: the preparation of tea waste fermentative prodn bacterial classification, get tea waste 4%, wheat bran 2% by weight percentage, and weight in wet base is 92% ~ 97%; The pH nature; 121 ℃ of sterilization 30 min, inoculating strain lys2207 is under 200 r/min conditions; 15L shaking culture 36 h, cell concentration reaches 1.2 * 10 in this seed culture fluid
9Cfu/mL, volume 8L is as the production bacterial classification of tea waste fermentation.
Embodiment 6: tea waste solid fermentation degraded, get tea waste 40% by weight percentage, weight in wet base is that 60%, 125 ℃ of sterilization 30 min processes tea waste solid fermentation substratum; Production bacterial classification with bacterial strain lys2207; Inoculation weight with 1.5% is inoculated in this tea waste solid fermentation substratum (12000kg); Mix, be piled into the ridge heap of high 0.6 m * wide 2.5m * long 10.0m, cover straw screen or mat above; 45 ℃ ~ 55 ℃ bottom fermentations 9 days, the solid tea waste that promptly gets detoxification after the drying and degraded; The product crude protein content is 9%, and free aminoacid content reaches 7.7%, has improved 43.5%, and crude fiber content is 21%; Reducing sugar content reaches 6.5%, has improved 16.4%; Saponin content 0.3% has reduced by 96.8%.
Embodiment 7: tea waste liquid fermenting degraded, get tea waste 10%, moisture 90% by weight percentage, and in the 100L fermentor tank, 121 ℃ of sterilization 30 min process the tea waste liquid fermentation medium; With the production bacterial classification of bacterial strain lys2207, with 1% inoculation weight be inoculated into and be equipped with in this tea waste liquid fermentation tank, 37 ℃ of following ventilating fermentations 5 days, the tea waste hydrolyzed solution that promptly gets detoxification after concentrating and degraded.The product crude protein content is 8.5%, and free aminoacid content reaches 7.5%, has improved 40.3%, and crude fiber content is 20%, and reducing sugar content reaches 6.1%, has improved 15.1%; Saponin content 0.2%.
Claims (3)
1. method of screening the microorganism strains of efficient degradation tea waste, its characteristic comprises following step:
⑴ the screening of high density tea saponin tolerance bacterial strain: compounding high concentration tea waste solid medium; Get tea waste 10% ~ 15%, agar 1.5% ~ 2.0% by weight percentage, water 85% ~ 90%, pH nature; 120 ~ 130 ℃ of sterilization 20 ~ 30 min; Utilize this screening of medium high density tea saponin tolerance bacterial strain, numbering, the screening that supplies further to obtain protein-high enzymic activity bacterial strain is used;
⑵ the screening of protein-high enzymic activity bacterial strain: preparation casein food grade solid medium, get casein food grade 1.0% ~ 2.0%, glucose 2.0% ~ 4.0% by weight percentage, agar 1.5% ~ 2.0%; Water 85% ~ 90%; PH 5 ~ 7, and 120 ~ 130 ℃ of sterilization 20 ~ 30 min utilize this substratum from high density tea saponin tolerance bacterial strain, further to filter out protein-high enzymic activity bacterial strain; Numbering supplies the further screening of screening high-cellulose enzymic activity bacterial strain to use;
⑶ the screening of high-cellulose enzymic activity bacterial strain: preparation CMC 99.5 solid medium, get CMC 99.5 1.0% ~ 1.5%, peptone 0.3% ~ 0.5% by weight percentage, agar 1.5% ~ 2.0%; Water 85% ~ 90%; PH 5 ~ 7, and 120 ~ 130 ℃ of sterilization 20 ~ 30 min utilize this substratum to tolerate bacterial strain and have from the high density tea saponin and further filter out high-cellulose enzymic activity bacterial strain the protein-high enzymic activity bacterial strain; Numbering, 4 ℃ of refrigerators are preserved subsequent use.
2. acquisition tea saponin height endurability according to claim 1, protein-high enzymic activity and high-cellulose enzymic activity bacterial strain, be used for further sieve again of shake flask fermentation degraded tea waste: its characteristic comprises: sieve again that substratum is got tea waste 2% ~ 5% by weight percentage, weight in wet base is 93% ~ 97%, the pH nature; The some bottles of 100 mL place 250 mL triangular flasks, 120 ~ 130 ℃ of sterilization 20 ~ 30 min; Inoculation step ⑶ obtains bacterial strain respectively; Under 200 ~ 300 r/min conditions, shaking table shaking culture 72 ~ 84 h select the bacterial strain that tea saponin content is low, free aminoacid content is high, reducing sugar content is high, the crude protein degradation rate is high, the robust fibre degradation rate is high, proteinase activity is high, cellulase activity is high; Numbering; As the production bacterial classification of tea waste fermentation, 4 ℃ of refrigerators are preserved, and are subsequent use.
3. acquisition tea saponin height endurability according to claim 2, protein-high enzymic activity and high-cellulose enzymic activity bacterium, the method for the tea waste that is used to degrade is characterized in that:
1. strain preparation: get tea waste 2% ~ 5%, wheat bran 1% ~ 2% by weight percentage; Weight in wet base is 93% ~ 97%, pH nature, 120 ~ 130 ℃ of sterilization 20 ~ 30 min; Inoculation step ⑶ obtains bacterial strain; Under 200 ~ 300 r/min conditions, shaking table shaking culture 24 ~ 36 h make that cell concentration reaches 10 in this seed culture fluid
8~ 10
9Cfu/mL is as the production bacterial classification of tea waste fermentation;
2. tea waste solid fermentation degraded: get tea waste 40% ~ 50% by weight percentage, weight in wet base is that 50% ~ 60%, 120 ~ 130 ℃ of sterilization 30 ~ 40min process tea waste solid fermentation substratum; Again 1. step being obtained bacterial strain is inoculated in this tea waste solid fermentation substratum with 1% ~ 3% inoculation weight; Mix; Be piled into high 0.5 ~ 0.8m, the ridge heap that length is not limit covers straw screen or mat above; 45 ℃ ~ 55 ℃ bottom fermentations 5 ~ 10 days, the solid tea waste that promptly gets detoxification after the drying and degraded;
3. tea waste liquid fermenting degraded: get tea waste 10% ~ 15% by weight percentage, weight in wet base is 85% ~ 90%, in 100L ~ 1000 L fermentor tanks, 120 ~ 130 ℃ of sterilization 30 ~ 40 min process the tea waste liquid fermentation medium; Again 1. step is obtained bacterial strain and be inoculated into 1% ~ 3% inoculation weight and be equipped with in this tea waste liquid fermentation tank, 32 ~ 37 ℃ of following ventilating fermentations 4 ~ 6 days, the tea waste hydrolyzed solution that promptly gets detoxification after concentrating and degraded.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865893A (en) * | 2012-12-13 | 2014-06-18 | 杭州益民化工有限公司 | Tea saponin decoloring enzyme and preparation method thereof |
| CN103950639A (en) * | 2014-05-20 | 2014-07-30 | 贵州大学 | Method of storing tea seed pancake by utilizing vacuum package |
| CN108865926A (en) * | 2018-06-07 | 2018-11-23 | 中国林业科学研究院亚热带林业研究所 | A kind of tannin and saponin microbial inoculum for degrading |
| CN116064282A (en) * | 2022-07-29 | 2023-05-05 | 贵州大学 | Fermentation technology for rapidly degrading tea saponin of cold pressed cake of oil tea and special fungus thereof |
-
2011
- 2011-12-26 CN CN2011104416510A patent/CN102517380A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865893A (en) * | 2012-12-13 | 2014-06-18 | 杭州益民化工有限公司 | Tea saponin decoloring enzyme and preparation method thereof |
| CN103950639A (en) * | 2014-05-20 | 2014-07-30 | 贵州大学 | Method of storing tea seed pancake by utilizing vacuum package |
| CN108865926A (en) * | 2018-06-07 | 2018-11-23 | 中国林业科学研究院亚热带林业研究所 | A kind of tannin and saponin microbial inoculum for degrading |
| CN108865926B (en) * | 2018-06-07 | 2021-04-20 | 中国林业科学研究院亚热带林业研究所 | Tannin and saponin degradation microbial agent |
| CN116064282A (en) * | 2022-07-29 | 2023-05-05 | 贵州大学 | Fermentation technology for rapidly degrading tea saponin of cold pressed cake of oil tea and special fungus thereof |
| CN116064282B (en) * | 2022-07-29 | 2024-04-05 | 贵州大学 | Fermentation technology for degrading tea saponin of cold pressed cake of oil tea and special fungus thereof |
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