CN102286553B - Method for preparing lactic acid by furfural slag and Chinese honey locust slag - Google Patents

Method for preparing lactic acid by furfural slag and Chinese honey locust slag Download PDF

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CN102286553B
CN102286553B CN201110257642.6A CN201110257642A CN102286553B CN 102286553 B CN102286553 B CN 102286553B CN 201110257642 A CN201110257642 A CN 201110257642A CN 102286553 B CN102286553 B CN 102286553B
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slag
furfural
honey locust
chinese honey
lactic acid
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CN102286553A (en
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蒋建新
赵丹青
齐祥
朱莉伟
邓立红
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Beijing Forestry University
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Beijing Forestry University
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Abstract

The invention provides a method for preparing lactic acid by furfural slag and Chinese honey locust slag, comprising the following steps of: neutralizing furfural slag aqueous solution by solid sodium hydroxide; filtering to obtain furfural slag filtrate and filtrate filter residue; extracting and separating saponin in Gleditsia australis by absolute ethyl alcohol to obtain Chinese honey locust slag; adding thermophilic lactic acid bacteria into the furfural slag filtrate to carry out adaptive culture to obtain a lactic acid bacteria strain; then, carrying out ultrasound stirring and mixing to the furfural slag and the Chinese honey locust slag; adding the lactic acid bacteria strain obtained by the adaptive culture; and obtaining the lactic acid by synchronous diastatic fermentation. The yield of the lactic acid prepared with the method is higher than the yield of the lactic acid prepared with the traditional cellulose fermentation method. Because the furfural slag and the Chinese honey locust slag serve as raw materials in the method for preparing lactic acid, residual saponin in the Chinese honey locust slag has good surfactant, raw material cost is greatly lowered, cellulose use amount is lowered, and the method is suitable for large-scale production.

Description

A kind of method of being prepared lactic acid by furfural dregs and Chinese honey locust slag
Technical field
The present invention relates to biomass chemical field, be specifically related to the method that Chinese honey locust slag and furfural dregs are prepared lactic acid.
Background technology
Lactic acid has another name called alpha-hydroxypropionic acid, molecular formula CH 3cHOHCOOH, relative molecular mass 90.08, is a kind of naturally occurring organic acid, is extensively present in human body, animal, plant and microorganism.
Lactic acid is lactic acid (C normally 3h 6o 3) and lactyllactic acid (C 6h 10o 5) mixture, be the viscosity liquid of water white transparency or buff syrup shape, odorless or micro-smelly with lipid acid almost, taste acid.
Lactic acid can be divided into technical grade, food grade and pharmacopeia level, wherein: the lactic acid content of technical grade is 50-90%; The lactic acid content of food grade is more than 80%; The lactic acid content of pharmacopeia level is 85-90%.
Lactic acid is in the application of food service industry, lactic acid has very strong anti-corrosive fresh-keeping effect, the tart flavour of lactic acid uniqueness can increase the delicious food of food, can be used as the soft drink of meticulous allotment and the first-selected acidic flavoring agent of fruit juice, add appropriate lactic acid to be conducive to improve beer quality, lactic acid powder can be used for the upper powder of all kinds of candies, as the acidic flavoring agent of powdery.Lactic acid is in the application of pharmaceutical industries, in the places such as ward, Operation theatre, laboratory, adopt lactic acid steam disinfection, aspect medical, be widely used as sanitas, carrier agent, solubility promoter, pharmaceutical preparation etc., poly(lactic acid) can be pumped into silk spinning and be become line as biocompatibility sutures, and lactic acid can directly be mixed with medicine or make lactic acid salt and use.Lactic acid is in the application of cosmetic industry, and Pfansteihl is widely used as the moisturizing agent of many skin care product, the pH adjusting agent of various hair products, the wetting Agent for Printing Inks of various bath wash supplies etc.In addition, lactic acid has application in biodegradable material, cigarette industry, textile industry, coating ink industry, tanning industry, microelectronics industry and agricultural.
The suitability for industrialized production of lactic acid mainly contains chemical synthesis, enzyme process and microbe fermentation method, and it is main that China adopts fermentation method more.Chemical synthesis, because raw material used is acetaldehyde and highly toxic substance prussic acid, makes synthesis method produce lactic acid and is greatly restricted, and its production cost is also higher in addition; Although Production by Enzymes lactic acid can obtain specificity optically-active lactic acid, technique more complicated, can the lactic acid that the method is produced be applied to the industrial further research that needs; Production by Microorganism Fermentation lactic acid, can obtain by the selection of strain improvement and culture condition having narrow spectrum Pfansteihl, D-ALPHA-Hydroxypropionic acid or DL-LACTIC ACID, can meet the Production requirement of poly(lactic acid) completely.
Tradition microbe fermentation method main raw material used is starch based, and cost is higher, and large-scale industrial production can consume a large amount of grain, the pressure of aggravation grain and feed resource.If can adopt lignocellulose raw material cheap and easy to get to produce, can not only expand source, the reduction raw materials cost of its raw material, and have positive effect to solving crisis in food and reducing environmental pollution, its economic benefit and social benefit are also fairly obvious.
The fiber of take is prepared lactic acid as raw materials through biotransformation good application prospect, but the subject matter that its industrialization faces comprises: efficient, the low-cost preconditioning technique of (1) cellulosic material; (2) cellulase consumption is large, and enzyme use cost is high; (3) because cellulose raw material specific volume is large, hydrolyzed solution sugar concentration is low, and in fermented liquid, product lactic acid concn is low; (4) the collection transportation radius of cellulosic material is large, and purchase acquisition cost is high.
At present, part be take agricultural crop straw and has been carried out the middle trial production of lactic product as raw material, about prepare the report of lactic acid with maize straw, there is China to brewage " research of maize straw simultaneous saccharification and fermentation production lactic acid " that within the 2008th, the 18th periodical is stepped on, maize straw concentration of substrate 30g/100ml, in product, lactic acid concn is 23.2g/l, lactic acid yield is 19.3%, and lactic acid yield is low; Product lactic acid concn 13.7g/l in " research that maize straw lactic fermentation residue is produced feed " that Heilongjiang University's natural science journal 4 periodicals in 2008 are stepped on, lactic acid yield is only 17%, lactic acid yield is lower.Because having complex construction and the Mierocrystalline cellulose itself that xylogen, Mierocrystalline cellulose, hemicellulose wrap up mutually, stalk there is stable crystalline texture, when producing lactic acid, simultaneous saccharification and fermentation utilize cellulase to be difficult to degraded cellulose, cellulase functioning efficiency is low, lactic acid yield is low, need to carry out complicated raw materials pretreatment.
Gleditsia officinalis well developed root system, drought-enduring water saving, solid good, be the ecological seeds of ideal of building farmland shelter belt, soil and water conservation forest and town and country Landscape Forest.There is wild Chinese honey locust woods in Shaanxi, Henan, Hebei, Sichuan, and artificial Chinese honey locust woods has been developed again in these areas at present, and cultivated area is over 500,000 mu.Seed content 15-30% in the full fruit of Chinese honey locust, is rich in high-quality polysaccharide gum in seed, its preparation technology has declared patent of invention (CN03132340.5); Pod content is 70-85%, saponin content 20-35% in pod, and Gleditisia sinensis Saponin can adopt dehydrated alcohol method to carry out high efficiency separation (CN0510011673.8).Separation and Extraction saponin produces a large amount of Chinese honey locust waste residues, accounts for the more than 50% of Chinese honey locust fruit.In addition, the 30 multinomial patents of relevant Chinese honey locust relate to directly utilizes Chinese honey locust raw material or the composite Chinese medicine of its aqueous extract, agricultural chemicals and articles for washing (CN1424102A, CN1301533A, CN1060313C etc.), and about the utilization of extracting the Chinese honey locust slag after saponin there are no report.
In the fibrous material boiling hydrolytic process of furfural production, with a large amount of furfuraldehyde waste slags, produce, furfural product per ton is discharged 10 tons of above residues (being furfural dregs), and China discharges approximately 3,000 ten thousand tons of furfural dregs every year.The production of furfural adopts weak acid water solution mostly, in separation in most of hemicellulose, complicated reticulated structure between the original Mierocrystalline cellulose of raw material and hemicellulose, xylogen suffers destruction to a certain degree, can save complicated plant fiber material preprocessing process, for utilizing biological conversion of furfural dregs Mierocrystalline cellulose that favourable condition is provided.
Because the Chinese honey locust waste residue producing after industrial extraction saponin is large, cheap with production furfural residue residue resource amount, the two contains abundant Mierocrystalline cellulose, its cellulosic degree of polymerization is lower and loosely organized, and in Chinese honey locust waste residue, also contain 5-8% Gleditisia sinensis Saponin, waste residue cellulose conversion lactic acid can save complicated plant fiber material preprocessing process, and the special construction of waste residue determine its be easy to by enzymic hydrolysis, hydrolysis cost low.
Summary of the invention
For the problem of above-mentioned existence, the object of the present invention is to provide a kind of method of being prepared lactic acid by furfural dregs and Chinese honey locust slag.
Provided by the inventionly by furfural dregs and Chinese honey locust slag, prepare the method that the method for lactic acid is simultaneous saccharification and fermentation.
The described method of preparing lactic acid comprises the steps:
1) with solid sodium hydroxide, the furfural dregs aqueous solution is neutralized, filtered, obtain furfural dregs filtrate and furfural filter residue;
2) with the saponin in the separated Chinese honey locust fruit of dehydrated alcohol extraction, obtain Chinese honey locust slag;
3) thermophilic type milk-acid bacteria being joined to step 1) in the furfural dregs filtrate that obtains, adaptability is cultivated, and obtains lactobacillus strain;
4) by step 1) the furfural filter residue and the step 2 that obtain) the Chinese honey locust slag ultrasonic agitation that obtains mixes, and obtains furfural filter residue and Chinese honey locust slag mixture;
5) by step 3) lactobacillus strain that obtains joins step 4) in the process ultrasonic agitation that obtains the furfural filter residue and Chinese honey locust slag mixture that mix, simultaneous saccharification and fermentation.
The pH of the furfural dregs aqueous solution neutralizing with sodium hydroxide described step 1) is controlled at 5-6; In the described furfural dregs aqueous solution, the mass ratio of furfural dregs and water is 1: 10.
Described step 2) in, dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, and minute three extracting and separating saponin at 50 ℃, filter and obtain Chinese honey locust slag.
Described step 3) culture temperature that the adaptability in is cultivated is 32 ℃, and incubation time is 24h, and described furfural dregs filtrate and the thermophilic type milk-acid bacteria envelope-bulk to weight ratio adding are 15: 1 (L/kg).
Described step 3) KH that also contains 0.5g/L in the furfural dregs filtrate in 2pO 4, 0.5g/L MgSO 4.7H 2the NaCl of O, 0.5g/L, and the CaCO of 20g/L 3.
Described step 4) it is 50-100g that the ultrasonic agitation in is mixed into Chinese honey locust slag and the total add-on of furfural filter residue in 1 liter of fermentation system, and wherein furfural filter residue and Chinese honey locust slag mass ratio are (10-30): 1.
Described step 4) in, to be mixed into Chinese honey locust slag and the total add-on of furfural filter residue in 1 liter of fermentation system be 50g to ultrasonic agitation, and wherein furfural filter residue and Chinese honey locust slag mass ratio are 28: 1.
Described step 4) in 1 liter of fermentation system, also add 4-12FPU/g Mierocrystalline cellulose to carry out ultrasonic agitation mixing.
Described step 4) in 1 liter of fermentation system, also add 12FPU/g Mierocrystalline cellulose to carry out ultrasonic agitation mixing.
Described step 4) in, ultrasonic frequency is 28kHz, and ultrasonic power is 100W, and the ultrasonication time is 5min.
Described step 5) in, the simultaneous saccharification and fermentation time is 48-96h, is preferably 96h, and temperature of reaction is 45 ℃, and pH value is 4.8, and the amount that joins the lactobacillus strain in furfural filter residue and 1 liter of fermentation system of Chinese honey locust slag mixture is 2-4g.
The furfural dregs that the present invention uses derives from the furfural dregs producing in furfural production process.In the fibrous material boiling hydrolytic process of furfural production, with a large amount of furfuraldehyde waste slags, produce, furfural product per ton is discharged 10 tons of above furfural dregs, and China discharges approximately 3,000 ten thousand tons of furfural dregs every year.The production of furfural adopts weak acid water solution mostly, in separation in most of hemicellulose, complicated reticulated structure between the original Mierocrystalline cellulose of raw material and hemicellulose, xylogen suffers destruction to a certain degree, can save complicated plant fiber material preprocessing process, for utilizing biological conversion of furfural dregs Mierocrystalline cellulose that favourable condition is provided.
The method of preparing lactic acid by furfural dregs and Chinese honey locust slag provided by the invention has the following advantages:
(1) extract in the Chinese honey locust slag that separated saponin crosses and contain abundant Mierocrystalline cellulose, and contain 5-8% triterpenes saponin; Due to xylogen and hemicellulose level low, Mierocrystalline cellulose is easy to occur bioconversion reaction, saponin is the high-quality natural surface active agent that promotes the reaction of biomass material enzymatic hydrolysis and fermentation.
(2) Technical furfural residue resource amount is large, cheap, and furfural dregs contains abundant Mierocrystalline cellulose, and its cellulosic degree of polymerization is lower and loosely organized, is to prepare one of biological preferred feedstock transforming of Mierocrystalline cellulose.
(3) two kinds of raw material synchronous saccharifications ferment altogether, have both fully transformed raw cellulose component, have also farthest utilized the residue saponin of Chinese honey locust slag simultaneously.
(4) the residue saponin in Chinese honey locust slag both can reduce the usage quantity of cellulase in synchronous saccharification ferments altogether, can shorten transformation time again.
(5) before synchronous saccharification ferments altogether, adopt ultrasonic stirring to mix, be conducive to the abundant mixing of raw material, saponin and enzyme, improve the sorption and desorption speed of cellulase, thereby improve reaction efficiency.
(6) adopt thermophilic type milk-acid bacteria, make lactic acid simultaneous saccharification and fermentation temperature of reaction (45 ℃) than 10 ℃ of left and right of ethanol simultaneous saccharification and fermentation temperature of reaction (35 ℃) rising, thereby improved reaction efficiency and reaction dress rate.
Accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is described in more detail.But these embodiment are for illustration the present invention, and these can not limit scope of the present invention.
Embodiment 1 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 5, then by the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, in 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) get the 48.3g furfural filter residue obtaining in the 1.7g Chinese honey locust slag that obtains in step 2 and step 1 and mix, add after 700mL water sterilizing 20min at 121 ℃, be cooled to room temperature, add 12FPU/g Mierocrystalline cellulose, then add aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 2.0g step 3 in step 4, at 45 ℃, carry out simultaneous saccharification and fermentation 96h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 36h to lactic acid concn.Lactic acid yield is 95.7%.
Embodiment 2 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 5, then to the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, in 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) get the 96.5g furfural filter residue obtaining in the 3.5g Chinese honey locust slag that obtains in step 2 and step 1 and mix, add after 700mL water sterilizing 20min at 121 ℃, be cooled to room temperature, add 12FPU/g Mierocrystalline cellulose, then add aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 4.0g step 3 in step 4, simultaneous saccharification and fermentation 96h, at 45 ℃, carry out simultaneous saccharification and fermentation 96h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 48h to lactic acid concn.Lactic acid yield is 92.5%.
Embodiment 3 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 5.5, then to the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, and 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) getting the 75.0g furfural filter residue obtaining in the 5.0g Chinese honey locust slag that obtains in step 2 and step 1 mixes, (Chinese honey locust slag and furfural filter residue total amount 80g) adds after 700mL water, sterilizing 20min at 121 ℃, be cooled to room temperature, add 8FPU/g Mierocrystalline cellulose, add again aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 3.0g step 3 in step 4, at 45 ℃, carry out simultaneous saccharification and fermentation 96h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 60h to lactic acid concn.Lactic acid yield is 72.5%.
Embodiment 4 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 5, then to the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, and 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) the 55.0g furfural filter residue obtaining in the 5.0g Chinese honey locust slag obtaining in step 2 and step 1 is mixed, (Chinese honey locust slag and furfural filter residue total amount 60g) adds after 700mL water, sterilizing 20min at 121 ℃, be cooled to room temperature, add 4FPU/g Mierocrystalline cellulose, add again aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 3.0g step 3 in step 4, simultaneous saccharification and fermentation 96h, temperature of reaction is 45 ℃ and carries out simultaneous saccharification and fermentation 96h, temperature of reaction is 45 ℃, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 72h to lactic acid concn.Lactic acid yield is 51.0%.
Embodiment 5 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 6, then to the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, and 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) get the 75.0g furfural filter residue obtaining in the 5.0g Chinese honey locust slag that obtains in step 2 and step 1 and mix (Chinese honey locust slag and furfural filter residue total amount 80g), add after 700mL water, sterilizing 20min at 121 ℃, be cooled to room temperature, add 8FPU/g Mierocrystalline cellulose, add again aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 3.0g step 3 in step 4, at 45 ℃, carry out simultaneous saccharification and fermentation 48h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid.Lactic acid yield is 61.0%.
Embodiment 6 prepares lactic acid by furfural dregs and Chinese honey locust slag
The lactic acid of the present embodiment is prepared as follows:
Step 1) with solid NaOH, the 100L furfural dregs aqueous solution (mass ratio of furfural dregs and water is 1: 10) is neutralized, until the pH value of the furfural dregs aqueous solution is 5, then to the furfural dregs aqueous solution centrifuging having neutralized, obtain furfural dregs filtrate and furfural filter residue.
Step 2) dehydrated alcohol and Chinese honey locust fruit mass ratio are 12: 1, and 50 ℃ minutes three times extracting and separating saponin, filter and obtain Chinese honey locust slag.
Step 3) get step 1) in the 15L furfural dregs filtrate that obtains, in this filtrate, add the KH of 7.5g 2pO 4, 7.5g MgSO 4.7H 2the NaCl of O, 7.5g, the CaCO of 300g 3, 1kg is thermophilic type milk-acid bacteria, under the culture temperature of 32 ℃, in fermentor tank, cultivate 24h, obtain lactobacillus strain.
Step 4) get the 70.0g furfural filter residue obtaining in the 5.0g Chinese honey locust slag that obtains in step 2 and step 1 and mix (Chinese honey locust slag and furfural filter residue total amount 75g), add after 700mL water, sterilizing 20min at 121 ℃, be cooled to room temperature, add 8FPU/g Mierocrystalline cellulose, add again aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28kHz, and the ultrasonic agitation time is 5min.
Step 5) in the mixed material of process ultrasonic agitation obtaining, add the lactobacillus strain obtaining in 3.0g step 3 in step 4, at 45 ℃, carry out simultaneous saccharification and fermentation 72h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 60h to lactic acid concn.Lactic acid yield is 70.6%.
Comparative example is prepared lactic acid with corn stalk powder
The lactic acid of this comparative example is prepared as follows:
Step 1) corn straw smashing is crossed to 20 mesh sieves, obtained corn stalk powder, get 50g corn stalk powder, add after 700mL water, at 121 ℃, sterilizing 20min, is cooled to room temperature, add 12FPU/g Mierocrystalline cellulose, then add aqua sterilisa to cumulative volume 1L, carry out ultrasonic agitation mixing.Ultrasonic power is 100W, and ultrasonic frequency is 28-kHz, and the ultrasonic agitation time is 5min.
Step 2) the thermophilic type milk-acid bacteria that adds 2.0g in step 1 in the mixed material of process ultrasonic agitation obtaining, at 45 ℃, carry out simultaneous saccharification and fermentation 96h, with the 10%NaOH aqueous solution, control pH value in reaction 4.8, make lactic acid, in fermentation system, to reach the peaked reaction times be 72h to lactic acid concn.Lactic acid yield is 23.6%.
From above-described embodiment and comparative example, by provided by the invention, by furfural dregs and Chinese honey locust, prepare lactic acid yield that the method for lactic acid prepares lactic acid far away higher than prepare the productive rate of lactic acid in prior art with maize straw.

Claims (6)

1. by furfural dregs and Chinese honey locust slag, prepared a method for lactic acid, it is characterized in that, the method is simultaneous saccharification and fermentation method, and the method comprises the steps:
1) with solid sodium hydroxide, the furfural dregs aqueous solution is neutralized, filtered, obtain furfural dregs filtrate and furfural filter residue;
2) with the saponin in the separated Chinese honey locust fruit of dehydrated alcohol extraction, obtain Chinese honey locust slag;
3) thermophilic type milk-acid bacteria is joined in the furfural dregs filtrate that step 1) obtains, adaptability is cultivated, and obtains lactobacillus strain;
4) the furfural filter residue and the step 2 that step 1) are obtained) the Chinese honey locust slag ultrasonic agitation that obtains mixes, and obtains furfural filter residue and Chinese honey locust slag mixture;
5) lactobacillus strain step 3) being obtained joins in the furfural filter residue and Chinese honey locust slag mixture that process ultrasonic agitation that step 4) obtains mixes, simultaneous saccharification and fermentation;
The pH of the furfural dregs aqueous solution wherein, neutralizing with sodium hydroxide in step 1) is controlled at 5-6; In the described furfural dregs aqueous solution, the mass ratio of furfural dregs and water is 1:10;
It is 50-100g that ultrasonic agitation in step 4) is mixed into Chinese honey locust slag and the total add-on of furfural filter residue in 1 liter of fermentation system, and wherein furfural filter residue and Chinese honey locust slag mass ratio are (10-30): 1;
In step 5), the simultaneous saccharification and fermentation time is 48-96h, and temperature of reaction is 45 ℃, and pH value is 4.8, and the amount that joins the lactobacillus strain in furfural filter residue and 1 liter of fermentation system of Chinese honey locust slag mixture is 2-4g.
2. the method for being prepared lactic acid by furfural dregs and Chinese honey locust slag according to claim 1, is characterized in that described step 2) in dehydrated alcohol and Chinese honey locust fruit mass ratio be 12:1, minute three extracting and separating saponin at 50 ℃, filtration obtains Chinese honey locust slag.
3. the method for being prepared lactic acid by furfural dregs and Chinese honey locust slag according to claim 1, it is characterized in that, the culture temperature that adaptability in described step 3) is cultivated is 32 ℃, and incubation time is 24h, and described furfural dregs filtrate and the thermophilic type milk-acid bacteria envelope-bulk to weight ratio L/kg adding are 15:1.
4. the method for being prepared lactic acid by furfural dregs and Chinese honey locust slag according to claim 1, it is characterized in that, in described step 4), to be mixed into Chinese honey locust slag and the total add-on of furfural filter residue in 1 liter of fermentation system be 50g to ultrasonic agitation, and wherein furfural filter residue and Chinese honey locust slag mass ratio are 28:1.
5. the method for being prepared lactic acid by furfural dregs and Chinese honey locust slag according to claim 1, is characterized in that, in described step 4), ultrasonic frequency is 28kHz, and ultrasonic power is 100W, and the ultrasonication time is 5min.
6. the method for being prepared lactic acid by furfural dregs and Chinese honey locust slag according to claim 1, is characterized in that, in described step 5), the simultaneous saccharification and fermentation time is 96h.
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