CN102329382A - Method for extracting rapeseed proteins through ultrasonic-microwave synergy - Google Patents
Method for extracting rapeseed proteins through ultrasonic-microwave synergy Download PDFInfo
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- CN102329382A CN102329382A CN201110259353A CN201110259353A CN102329382A CN 102329382 A CN102329382 A CN 102329382A CN 201110259353 A CN201110259353 A CN 201110259353A CN 201110259353 A CN201110259353 A CN 201110259353A CN 102329382 A CN102329382 A CN 102329382A
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Abstract
The invention develops new technology for extracting rapeseed proteins through ultrasonic-microwave synergy. By the new technology, the extraction process parameters are optimized, the proteins in rapeseed cakes can be quickly dissolved out, the extraction time is shortened, the protein extraction efficiency is effectively improved, energy is saved and consumption is reduced at the same time and the production cost is lowered. In the invention, the high temperature rapeseed cakes with hulls are taken as raw materials. The method comprises the following steps: taking 40-100-mesh rapeseed meal, adding distilled water according to solid-liquid ratio of 1:20, adjusting the pH value to 8-12 with NaOH and carrying out soaking for 5-25 minutes; through ultrasonic-microwave synergistic extraction, carrying out extraction for 5-9 minutes under the conditions of ultrasonic frequency of 40kHz, power of 50W and microwave power of 80W, carrying out extraction three times, combining the extracts and centrifuging the combined extract to obtain supernatant; and carrying out acid precipitation under the condition of pH value of 4.5 and preparing the rapeseed proteins through centrifugal separation and drying. Compared with the traditional aqueous phase extraction method, the method provided by the invention has the following advantages: the extraction ratio is improved by 7.74%; and the extraction time is shortened by 5 times.
Description
One, technical field
The present invention provides a kind of process method that adopts ultrasonic-microwave cooperating to extract rape seed protein, belongs to technical field of agricultural product process.
Two, background technology
Semen Brassicae campestris is a kind of important oil crops, and the cultivated area of China's Semen Brassicae campestris is more than 100,000,000 mu, and output is up to more than 1,000 ten thousand tons, first of the Jun Ju world.Ten thousand tons of the rapeseed meal 600-700 that produces in the annual course of processing use but be used as fertilizer more than 80%.Rich in protein in the Semen Brassicae campestris reaches 25%-27%, and protein is up to 35%-45% in the rapeseed meal behind the immersion oil; And amino acid whose kind is abundant in the rapeseed meal; Contain 8 kinds of indispensable amino acids, can satisfy the needs of human physiological functions, do not have limiting amino acid; Its amino acid is formed and content meets FAO/WHO recommendation mode value; Compare with other plant albumen such as soybean, peanut, cottonseeds, its biological value and nutritive value are higher, are a kind of quality plant protein with potentiality to be exploited.
Whether the Semen Brassicae campestris dregs of rice peel according to vegetable seed can be divided into the decortication dregs of rice and the belt leather dregs of rice; Different according to vegetable seed system oil treatment mode, again rapeseed meal is divided into the high temperature dregs of rice and the low temperature dregs of rice.Because the vegetable seed peeling technology is loaded down with trivial details, lose greatlyyer, and the pyroprocessing oil yield is high, profit is big, therefore domestic oily factory adopts belt leather high temperature to squeeze mostly, thereby produces a large amount of high temperature belt leather rapeseed meal.At present, domestic research about low temperature decortication rapeseed meal is more, and it is comparatively desirable to extract the albumen effect, but does not appear in the newspapers about the development and use of high temperature belt leather rapeseed meal.Its major cause is, sex change at high temperature takes place the albumen part of high temperature belt leather rapeseed meal, is difficult for extracting, and some albumen combines with Mierocrystalline cellulose, is difficult to discharge.
At present; The method of extracting rape seed protein has water extraction process, organic solvent method, water enzymolysis process, ultrafiltration, percolation process etc., and water extraction process extraction time in industrial production is longer, and protein extracting ratio is not high; Also have waste water to be difficult for handling, be prone to cause problems such as environmental pollution; Prepare the method for rape seed protein with ethanol, acetone and other organic solvent, complex process, cost height and solvent are to protein nutritive value and edible safety influence etc.; The water enzymolysis process can improve the extraction yield of rape seed protein, but product is main with protein peptide, and functional propertys such as proteic emulsification and foaming reduce; Ultrafiltration, percolation process also are applied to separate preparation rape seed protein liquid gradually; But it is contaminated that ultrafiltration face or fenestra are easy to; Cause the processing power of ultra-filtration membrane to descend even can not work; And the cleaning after polluting is also quite numerous and diverse, causes the promotion and application of ultra-filtration technique in industry to be very limited.
Extracting rape seed protein with ultrasonic-microwave cooperating is a kind of emerging method; Its principle is that two kinds of modes of action of ultrasonic vibration and open microwave are combined; Overcome the deficiency of conventional ultrasound and microwave extracting, realized low-temperature atmosphere-pressure sample pre-treatment rapidly and efficiently, make heat effect, mechanical effect, cavatition and many second-order effects as smash, enhancing such as emulsification, diffusion; And can effectively break the cell boundaries layer; Velocity of diffusion is increased, promote effective constituent stripping in the solid materials, significantly improve its extraction efficiency.Extract the research of rape seed protein at present for ultrasonic-microwave cooperating, do not appear in the newspapers as yet.
Three, summary of the invention
Technical problem the present invention adopts ultrasonic and microwave cooperating extraction rape seed protein, adopts this method, can significantly shorten extraction time, improves the rape seed protein extraction efficiency, has the vast market development prospect.
Technical scheme adopts ultrasonic and microwave cooperating to extract the technology of rape seed protein, mainly comprise following some:
1, the pre-treatment of dregs of rapeseed cake
Rapeseed cake dregs is pulverized, crossed the 40-100 mesh sieve, add water at 1: 20 by solid-liquid ratio then and mix, use NaOH to transfer to pH and be 8-12, stir, soak 20min.
2, ultrasonic-microwave cooperating extracts rape seed protein
Above-mentioned soak solution is put into ultrasonic-microwave synergistic extraction appearance, open supersonic switch (natural frequency 40kHz, power are 50w), it is 80w that microwave power is set, and extracts 7-9min then.
3, repeat above-mentioned 1,2 steps and extract 3 times, centrifugal 10min under the 3500r/min condition, supernatant is a protein extract.The extraction yield of rape seed protein can be calculated as follows:
Protein content in protein content/raw material in protein extracting ratio=extracting solution * 100%.
4, acid is heavy
PH transfers to 4.5 with extracting solution, centrifugal 10min under the 3500r/min condition then, and the lower sediment thing is a rape seed protein.
5, lyophilize
The pH of above-mentioned rape seed protein is transferred to 7.0, behind the pre-freeze 24h, carries out vacuum lyophilization, obtain protein product, with nitrogen determination (with reference to GB: GB/T5009.5-2003) measure protein content.
Four, embodiment
Below in conjunction with specific embodiment the present invention is described further:
Embodiment 1
Rapeseed cake dregs is pulverized, crossed 60 mesh sieves, take by weighing 10g and put into beaker; Add water in 1: 20 ratio and mix, stir, using NaOH to transfer to pH is 12; Soak 20min, solution is put into ultrasonic-microwave synergistic extraction appearance, open supersonic switch (natural frequency 40kHz; Power is 50w), it is 80w that microwave power is set, be 500s action time; Filter, filter residue is added water in 1: 10 ratio mix, transferring pH is 12, stirs, and puts into ultrasonic-microwave synergistic extraction appearance again, handles 7-9min by above-mentioned similarity condition; Filter residue merges 3 times filtrating after handling 2 times, centrifugal 10min under the 3500r/min condition, the centrifugal protein extract that gets; Is that acid is heavy under 4.5 conditions with supernatant at pH, at last the heavy albumen of acid is carried out lyophilize.The extraction yield that obtains rape seed protein is 90.54%, and it is 78.2% that nitrogen determination records proteic purity.Compare with traditional water extraction method, proteic extraction yield has improved 7.74%, and extraction time has shortened 5 times.
Claims (3)
- An employing ultrasonic-microwave synergistic extraction extracts the process method of rape seed protein; It is characterized in that adopting following steps: (1) is raw material with high temperature belt leather rapeseed cake dregs; With its crushing screening, get 60 order rapeseed meal, be 1: 20 adding zero(ppm) water by solid-liquid ratio; Use alkali lye to regulate pH and be 8-12, soak 5-25min; (2) (1) solution is put into ultrasonic-microwave synergistic extraction appearance, open supersonic switch (natural frequency 40kHz, power are 50w), it is 80w that microwave power is set, and be 5-9min action time, extracts 3 times, centrifugal; (3) with the supernatant of (2) in pH 4.5 condition settle, centrifugal then, at last sedimentary albumen is carried out lyophilize.
- 2. according to claim 1, rapeseed meal is got the 40-100 order as raw material through crushing screening; The ratio of rapeseed meal and water is 1: 20; Regulating the used alkali lye of pH is NaOH or Na 2CO 3, the pH of extracting solution is 8-12.
- 3. according to claim 1, UW and microwave act on simultaneously in the leaching process of rape seed protein; Frequency of ultrasonic is 40KHz, and power is 50w, and microwave power is 80w; Ultrasonic-microwave cooperating extraction time is 5-9min.
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| CN201110259353A CN102329382A (en) | 2011-09-05 | 2011-09-05 | Method for extracting rapeseed proteins through ultrasonic-microwave synergy |
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| CN201110259353A CN102329382A (en) | 2011-09-05 | 2011-09-05 | Method for extracting rapeseed proteins through ultrasonic-microwave synergy |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558381A (en) * | 2012-02-16 | 2012-07-11 | 上海理工大学 | Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism |
| CN103518943A (en) * | 2013-10-20 | 2014-01-22 | 丹阳市正大油脂有限公司 | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves |
| CN104431285A (en) * | 2014-11-04 | 2015-03-25 | 南昌大学 | Method for preparing ovalbumin with high foamability by microwave ultrasonic waves |
| CN104447945A (en) * | 2014-12-30 | 2015-03-25 | 江苏天琦生物科技有限公司 | Seed cake protein ultrasonic assisted extraction system |
| CN105475487A (en) * | 2015-12-09 | 2016-04-13 | 中国农业大学 | Preparation method of ginkgo protein peptide fermented milk and ginkgo protein peptide fermented milk beverage |
| CN105779543A (en) * | 2016-04-13 | 2016-07-20 | 武汉轻工大学 | Method for preparing organic rapeseed protein through enzyme method |
| CN105777860A (en) * | 2016-04-07 | 2016-07-20 | 华南农业大学 | Microwave assisted process method for extracting tea residue protein |
| CN106107014A (en) * | 2016-07-04 | 2016-11-16 | 武汉轻工大学 | The method of compound physical field coordination strengthening rapeseed protein glycosylation modification |
| CN107114799A (en) * | 2017-06-05 | 2017-09-01 | 江苏大学 | The ultrasonic preparation method of rapeseed protein microcapsules and the application as functional food |
| CN107298773A (en) * | 2017-06-05 | 2017-10-27 | 江苏大学 | The frequency sweep ultrasonic ripple preparation method of rapeseed protein chitosan nano particle |
| CN113372409A (en) * | 2021-06-08 | 2021-09-10 | 山东农业工程学院 | Method for extracting tenebrio molitor protein by ultrafine grinding-ultrasonic wave-microwave coupling |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558381B (en) * | 2012-02-16 | 2013-11-06 | 上海理工大学 | Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism |
| CN102558381A (en) * | 2012-02-16 | 2012-07-11 | 上海理工大学 | Method for extracting water-soluble soybean polysaccharide from low-temperature bean pulp through microwave-ultrasonic synergism |
| CN103518943A (en) * | 2013-10-20 | 2014-01-22 | 丹阳市正大油脂有限公司 | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves |
| CN104431285A (en) * | 2014-11-04 | 2015-03-25 | 南昌大学 | Method for preparing ovalbumin with high foamability by microwave ultrasonic waves |
| CN104447945A (en) * | 2014-12-30 | 2015-03-25 | 江苏天琦生物科技有限公司 | Seed cake protein ultrasonic assisted extraction system |
| CN105475487A (en) * | 2015-12-09 | 2016-04-13 | 中国农业大学 | Preparation method of ginkgo protein peptide fermented milk and ginkgo protein peptide fermented milk beverage |
| CN105777860A (en) * | 2016-04-07 | 2016-07-20 | 华南农业大学 | Microwave assisted process method for extracting tea residue protein |
| CN105779543A (en) * | 2016-04-13 | 2016-07-20 | 武汉轻工大学 | Method for preparing organic rapeseed protein through enzyme method |
| CN106107014A (en) * | 2016-07-04 | 2016-11-16 | 武汉轻工大学 | The method of compound physical field coordination strengthening rapeseed protein glycosylation modification |
| CN106107014B (en) * | 2016-07-04 | 2019-08-30 | 武汉轻工大学 | The method of compound physical field cooperative reinforcing rapeseed protein glycosylation modification |
| CN107114799A (en) * | 2017-06-05 | 2017-09-01 | 江苏大学 | The ultrasonic preparation method of rapeseed protein microcapsules and the application as functional food |
| CN107298773A (en) * | 2017-06-05 | 2017-10-27 | 江苏大学 | The frequency sweep ultrasonic ripple preparation method of rapeseed protein chitosan nano particle |
| CN113372409A (en) * | 2021-06-08 | 2021-09-10 | 山东农业工程学院 | Method for extracting tenebrio molitor protein by ultrafine grinding-ultrasonic wave-microwave coupling |
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Application publication date: 20120125 |