CN103704462B - Extraction and purification method for sesame proteins - Google Patents
Extraction and purification method for sesame proteins Download PDFInfo
- Publication number
- CN103704462B CN103704462B CN201410004150.XA CN201410004150A CN103704462B CN 103704462 B CN103704462 B CN 103704462B CN 201410004150 A CN201410004150 A CN 201410004150A CN 103704462 B CN103704462 B CN 103704462B
- Authority
- CN
- China
- Prior art keywords
- sesame
- protein
- extraction
- alkali
- centrifugal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses an extraction and purification method for sesame proteins. According to the extraction and purification method, in an extraction step, rough proteins of sesames are extracted from squeezed sesame cakes through an alkali dissolution and acid deposition method, and the alkali dissolution is carried out while ultrasonic treatment is carried out; in the purification process, a saccharifying enzyme treatment process is applied to the purification of the rough proteins of the sesames. According to the extraction and purification method provided by the invention, the yield of the sesame proteins is high, the purity is high and the product quality is good; the extraction and purification method is easy to popularize; the method is simple in process and can realize continuous production; the utilization rate and the additional value of the sesames are improved.
Description
technical field
The invention belongs to and utilize biotechnology to carry out food production field, particularly relate to a kind of extraction that utilizes biology enzyme to carry out sesame protein, purification process.
background technology
Sesame protein is the good albumen of a kind of complementarity, and it is also higher than soybean protein that it contains sulfur-containing amino acid.Sesame cake meal and protein isolate thereof all contain a large amount of methionine (2.5%～4.0%) and sulfur-containing amino acid (3.8%～5.5%); Lysine is the first limiting amino acid, though its content of most of kind not as good as standard, in Semen sesami nigrum, content is higher.Sesame protein efficiency ratio (PER) is 1.86, and sesame cake meal is 1.35; Protein isolate biological value is 62.4%, lower than soybean and sunflower seeds; Clean protein utilization rate is 53.4%, is equivalent to milk 81.0%.Evans finds that a sesame protein admixes a soybean protein and can have nutritive value identical with casein.Soybean protein lysine is high, methionine is low, just in time complementary with sesame protein, makes both to improve nutritive value.With sesame seed meal, make bean food admixture, than more fully discharging amino acid with methionine in alimentary canal, with albumen, supplement albumen, amino acid supplementation albumen, can provide other essential amino acid amount and increase albumen use amount individually.Therefore, the sesame cake meal of take extracts albumen as raw material, develops and utilization is very significant.Traditional take sesame cake meal to prepare sesame protein impurity many as raw material, purity is low.
summary of the invention
The extraction, the purification process that the object of this invention is to provide a kind of sesame protein, the productive rate of extraction is high, and the sesame protein quality obtaining is good, and has efficiently utilized sesame cake meal, has improved the utilization rate of sesame.
In order to solve the problems of the technologies described above, the invention provides a kind of extraction, purification process of sesame protein, it is characterized in that: comprise the following steps:
1) extract the sesame-send cake after squeezing is extracted to sesame crude protein by alkali extraction and acid precipitation, first sesame-send cake added to aqueous dispersion and grind, then adjust pH is 9.5 ~ 10.5 at 50 ℃ ~ 60 ℃, to carry out alkali and carry, alkali carry carry out simultaneously ultrasonic, then centrifugal, get centrifugal sediment and adjust pH to repeat abovementioned steps to carry out again alkali and mention centrifugally, the centrifugate adjust pH finally obtaining is 4.0 ~ 4.5, successively through standing, centrifugal, be dissolved in water, centrifugal, washing postlyophilization obtains sesame crude protein;
2) purifying by sesame crude protein first by being dissolved in water, then adjust pH to 3.9 ~ 4.1, at temperature 50 C ~ 60 ℃, add the carbohydrase of its weight 0.02% ~ 0.04% to be hydrolyzed, enzyme precipitate centrifugal goes out after hydrolysis, the sesame protein of gained repeats aforementioned technique again, finally obtains the sesame protein after purifying.
In described leaching process, the time that alkali is carried is for the first time 1.5-2h, and the time that alkali is carried is for the second time 0.5-1h.By twice alkali, carry and ultrasonic wave assisted extraction, can improve dissolved rate of protein.
In described leaching process, the mass ratio of sesame cake meal and water is 1:9 ~ 12, preferably 1:10.When solid-liquid ratio is too small, sesame protein can not be well-dispersed in water; The increase of amount of liquid not only can make protein dissolve to greatest extent, and the concentration of extract protein is low, and under heating condition, protein molecule collides mutually, and the chance of winding is few, and the protein that launches shape is difficult for wadding collection, and protein content reduces on the contrary.
In described leaching process, hyperacoustic power is 180-200W, and by the ultrasonic processing of this frequency, the penetration power that increases intermolecular warm-up movement and increase medium promotes the stripping of albumen in sesame cake meal.
In described leaching process, before freeze drying, sesame crude protein pH should be adjusted to 6.8 ~ 7.2; In described purge process, after the enzyme that goes out, regulating pH is 6.8 ~ 7.2.Because protein is amphiprotic substance, in acidity or alkali condition, all can be hydrolyzed to polypeptide, after extraction and purifying, pH is adjusted to neutrality, can guarantee product yield and purity, reduce the existence of sesame polypeptide in product.
In described purge process, while being dissolved in water, water is 10:1 with the weight of material ratio of sesame crude protein.
In described purge process, the time that adds enzyme hydrolysis is 0.5 ~ 1h.
In leaching process, the pH that alkali is carried is 9.5; During centrifugate precipitation, pH is 4.5.
In purge process, pH is adjusted to 4.0.
The present invention also has following beneficial effect:
1, the present invention carries in sesame protein process and carries out ultrasonic processing at alkali, utilizes ultrasonic wave to have mechanical effect, cavitation effect and fuel factor, and by increasing the warm-up movement between medium molecule, and the penetration power of increase medium is extracted effective components in plants; Effectively promote the stripping of albumen, improve dissolved rate of protein; And alkali is carried in process, adopt twice alkali to carry, also can extract the sesame protein in sesame cake meal as far as possible.
2, compare conventional production methods, the present invention processes carbohydrase to be applied in the purification of sesame protein first, and after processing with carbohydrase, sesame protein content has remarkable rising; Carbohydrase claims again glucoamylase, and carbohydrase is a kind of customary title, and formal name used at school is α-1,4-glucose hydrolysis enzyme, it can produce glucose from irreducibility end hydrolysis a-1.4 glucoside bond starch, also can slowly be hydrolyzed a-1.6 glucoside bond, is converted into glucose.Also can be hydrolyzed dextrin, the non-reduced end of glycogen discharges β-D-Glucose simultaneously.Glucose can be water-soluble, and sesame protein is water insoluble, thus can be separated with sesame protein, by carbohydrase, get rid of the part glucide in sesame protein, improve sesame protein content.When without enzymolysis, sesame protein content is 40% left and right, and after processing through a carbohydrase, sesame protein content is more than 80%, and after twice saccharification, protein content can be to more than 90%.
3, the present invention utilizes sesame cake meal to extract albumen for raw material, can turn waste into wealth, and increases value-added content of product, improves the utilization rate of sesame; And method provided by the invention can realize continuous production, lose little, with short production cycle.
4, the sesame protein obtaining by method provided by the invention, its purity is high, and good product quality can be made into pulvis directly edible, or is further processed as health food, and edibility is high.
The specific embodiment
Below in conjunction with embodiment, further illustrate the present invention, but the scope of protection of present invention is not limited to the scope of embodiment statement.
Embodiment 1:
The extraction of sesame protein, a purification process, comprise the following steps:
1) extract and according to solid-liquid ratio 1:10, sesame-send cake and distilled water are mixed, ground, then adjust pH is 9.5, and at ultrasonic wave 180W, under 50 ℃ ~ 60 ℃ conditions, alkali is carried 1.5h, then centrifugal; Get centrifugal sediment and carry out alkali for the second time and carry 1h, it is constant that other alkali are put forward condition, then centrifugal, regulates centrifugate pH to be 4.5 standing 1h, and centrifugal, taking precipitate, adds water washing to pH6.8 ~ 7.2, and freeze drying obtains sesame crude protein;
2) purifying is dissolved in water sesame crude protein according to solid-liquid ratio 1:10, then adjust pH to 4.0, in the time of 50 ~ 60 ℃, add the carbohydrase of sesame crude protein quality 0.02% to be hydrolyzed, enzyme precipitate centrifugal goes out after hydrolysis, the sesame protein of gained repeats aforementioned purifying process again, last taking precipitate, adds water washing to pH6.8 ~ 7.2, obtain purifying good sesame protein.
Through check, its purity is 90.6%, and the purity of the sesame protein that traditional handicraft is produced is general all lower than 80%.
Embodiment 2:
The extraction of sesame protein, a purification process, comprise the following steps:
1) extract and according to solid-liquid ratio 1:10, sesame-send cake and distilled water are mixed, ground, then adjust pH is 10, and at ultrasonic wave 200W, under 58 ℃ of conditions, alkali is carried 2h, then centrifugal; Get centrifugal sediment and carry out alkali for the second time and carry 1h, it is constant that other alkali are put forward condition, then centrifugal, regulates centrifugate pH to be 4.0 standing 0.8h, and centrifugal, taking precipitate, adds water washing to pH7.1, and freeze drying obtains sesame crude protein;
2) purifying is dissolved in water sesame crude protein according to solid-liquid ratio 1:10, then adjust pH to 3.9, in the time of 55 ℃, add the carbohydrase of sesame crude protein quality 0.03% to be hydrolyzed, enzyme precipitate centrifugal goes out after hydrolysis, the sesame protein of gained repeats aforementioned purifying process again, last taking precipitate, adds water washing to pH7.0, obtain purifying good sesame protein.
Through check, its purity is 92.3%.
Implement 3:
The extraction of sesame protein, a purification process, comprise the following steps:
1) extract and according to solid-liquid ratio 1:10, sesame-send cake and distilled water are mixed, ground, then adjust pH is 10.5, and at ultrasonic wave 190W, under 60 ℃ of conditions, alkali is carried 2h, then centrifugal; Get centrifugal sediment and carry out alkali for the second time and carry 0.5h, it is constant that other alkali are put forward condition, then centrifugal, regulates centrifugate pH to be 4.3 standing 0.5h, and centrifugal, taking precipitate, adds water washing to pH6.9, and freeze drying obtains sesame crude protein;
2) purifying is dissolved in water sesame crude protein according to solid-liquid ratio 1:10, then adjust pH to 4.0, in the time of 60 ℃, add the carbohydrase of sesame crude protein quality 0.04% to be hydrolyzed, enzyme precipitate centrifugal goes out after hydrolysis, the sesame protein of gained repeats aforementioned purifying process again, last taking precipitate, adds water washing to pH7.0, obtain purifying good sesame protein.
Through check, its purity is 93.1%.
Claims (7)
1. the extraction of sesame protein, a purification process, is characterized in that: comprise the following steps:
1) extract the sesame-send cake after squeezing is extracted to sesame crude protein by alkali extraction and acid precipitation, first sesame-send cake added to aqueous dispersion and grind, the mass ratio of sesame-send cake and water is 1:9 ~ 12, then adjust pH is 9.5 at 50 ℃ ~ 60 ℃, to carry out alkali and carry, alkali carry carry out simultaneously ultrasonic, then centrifugal, getting centrifugal sediment adjusts pH to repeat abovementioned steps to carry out again alkali and mention centrifugal, the time that alkali is carried is for the first time 1.5-2h, the time that alkali is carried is for the second time 0.5-1h, the centrifugate adjust pH finally obtaining is 4.5, pass through successively standing, centrifugal, be dissolved in water, centrifugal, washing postlyophilization obtains sesame crude protein,
2) purifying by sesame crude protein first by being dissolved in water, water is 10:1 with the weight of material ratio of sesame crude protein, then adjust pH to 3.9 ~ 4.1, at temperature 50 C ~ 60 ℃, add the carbohydrase of its weight 0.02% ~ 0.04% to be hydrolyzed, enzyme precipitate centrifugal goes out after hydrolysis, the sesame protein of gained repeats aforementioned technique again, finally obtains the sesame protein after purifying.
2. the extraction of sesame protein, purification process according to claim 1, is characterized in that: in described leaching process, the mass ratio of sesame cake meal and water is 1:10.
3. the extraction of sesame protein, purification process according to claim 1, is characterized in that: in described leaching process, hyperacoustic power is 180-200W.
4. the extraction of sesame protein, purification process according to claim 1, is characterized in that: in described leaching process, before freeze drying, sesame crude protein pH should be adjusted to 6.8 ~ 7.2.
5. the extraction of sesame protein, purification process according to claim 1, is characterized in that: in described purge process, the time that adds enzyme hydrolysis is 0.5 ~ 1h.
6. the extraction of sesame protein, purification process according to claim 1, is characterized in that: in described purge process, after the enzyme that goes out, regulating pH is 6.8 ~ 7.2.
7. according to the extraction of one of claim 1-6 described sesame protein, purification process, it is characterized in that: in purge process, pH is adjusted to 4.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004150.XA CN103704462B (en) | 2014-01-06 | 2014-01-06 | Extraction and purification method for sesame proteins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004150.XA CN103704462B (en) | 2014-01-06 | 2014-01-06 | Extraction and purification method for sesame proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103704462A CN103704462A (en) | 2014-04-09 |
| CN103704462B true CN103704462B (en) | 2014-12-10 |
Family
ID=50398136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410004150.XA Expired - Fee Related CN103704462B (en) | 2014-01-06 | 2014-01-06 | Extraction and purification method for sesame proteins |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103704462B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814246A (en) * | 2015-04-03 | 2015-08-05 | 安徽新桥工贸有限责任公司 | Extraction method of sesame cake meal protein power |
| CN104824334A (en) * | 2015-04-03 | 2015-08-12 | 安徽新桥工贸有限责任公司 | Method for supersonic wave-assistant extraction of sesame protein |
| CN104814247A (en) * | 2015-04-03 | 2015-08-05 | 安徽新桥工贸有限责任公司 | Method for extracting protein powder in sesame seed meal by alkali liquid |
| CN105124132B (en) * | 2015-08-07 | 2018-06-29 | 河南省农业科学院 | A kind of method of albumen in ultrasonic wave auxiliary weak base extraction cold press sesame cake meal |
| CN105419933A (en) * | 2015-11-18 | 2016-03-23 | 武汉轻工大学 | Preparation method of fragrant sesame oil |
| CN105660987A (en) * | 2016-01-20 | 2016-06-15 | 山东禹王生态食业有限公司 | Pipe bundle device and method for extracting isolated soybean protein in ultrasonic-assisted manner |
| CN109645212A (en) * | 2019-01-22 | 2019-04-19 | 武汉轻工大学 | A kind of preparation method of sesame protein |
| CN114027387A (en) * | 2021-11-24 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content liquorice protein |
| CN114027388A (en) * | 2021-11-25 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content baicalin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224022A (en) * | 2007-01-15 | 2008-07-23 | 天津科技大学 | Processing method for simultaneously preparing sesame oil and protein by water enzymatic extraction |
| CN101423860A (en) * | 2008-12-11 | 2009-05-06 | 江苏大学 | Gingeli antioxidation polypeptide composition and method for producing the same |
| CN103478400A (en) * | 2013-09-11 | 2014-01-01 | 无锡群硕谷唐生物科技有限公司 | High-purity rice bran protein and rice bran fibers and preparation method thereof |
-
2014
- 2014-01-06 CN CN201410004150.XA patent/CN103704462B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224022A (en) * | 2007-01-15 | 2008-07-23 | 天津科技大学 | Processing method for simultaneously preparing sesame oil and protein by water enzymatic extraction |
| CN101423860A (en) * | 2008-12-11 | 2009-05-06 | 江苏大学 | Gingeli antioxidation polypeptide composition and method for producing the same |
| CN103478400A (en) * | 2013-09-11 | 2014-01-01 | 无锡群硕谷唐生物科技有限公司 | High-purity rice bran protein and rice bran fibers and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103704462A (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103704462B (en) | Extraction and purification method for sesame proteins | |
| CN104161259B (en) | A kind of breaking yeast cellule membrane also prepares the method for yeast extract | |
| CN101096696B (en) | Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process | |
| CN102308903B (en) | Industrialized production method of corn polypeptide | |
| CN103571609A (en) | Method for synchronously preparing high-stability rice bran oil and rice bran protein | |
| CN105779542B (en) | A kind of method that enzyme process prepares organic rapeseed peptides | |
| CN103305576B (en) | Process for preparing corn peptides by taking corn starch sugar residues as raw materials | |
| CN101077118A (en) | Method for preparing soluble heart trefoil protein | |
| CN102077898A (en) | Peanut protein concentrate preparation method | |
| CN103014108A (en) | Preparation method of corn oligopeptide | |
| CN110734948A (en) | extraction device and process for extracting selenium polypeptide from soybeans | |
| CN102154422B (en) | Method for extracting functional small peptides from low-value sea fish | |
| CN103125735A (en) | Preparation method of walnut polypeptide | |
| CN102329382A (en) | Method for extracting rapeseed proteins through ultrasonic-microwave synergy | |
| CN103436581A (en) | Method for extracting rapeseed peptides through wet milling combined with enzymic method | |
| CN104187924A (en) | Preparation method of corn oligopeptide powder | |
| CN103694374A (en) | Method for extracting agar, fucoidin and protein from gracilaria as raw material by using enzyme process | |
| CN103710415A (en) | Method for preparing corn peptide by using corn gluten water | |
| CN103045705A (en) | Method for producing protein peptide composed of fish protein peptide and soybean peptide | |
| CN106666071A (en) | Method for preparing flavor peptide from vegetable protein | |
| CN102150806A (en) | Method for producing novel ossein flavor powder through ultrasonic auxiliary spray drying | |
| CN103518943A (en) | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves | |
| CN110713514A (en) | Extraction method of wheat small molecule active peptide | |
| CN104206640A (en) | Process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae | |
| CN110897059A (en) | Method for preparing plant hydrolyzed protein beverage by using wheat germs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20180106 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |