CN101077118A - Method for preparing soluble heart trefoil protein - Google Patents
Method for preparing soluble heart trefoil protein Download PDFInfo
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- CN101077118A CN101077118A CN 200710023257 CN200710023257A CN101077118A CN 101077118 A CN101077118 A CN 101077118A CN 200710023257 CN200710023257 CN 200710023257 CN 200710023257 A CN200710023257 A CN 200710023257A CN 101077118 A CN101077118 A CN 101077118A
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Abstract
The soluble cloverleaf protein preparing process belongs to the field of vegetable protein producing technology. The soluble cloverleaf protein preparing process with compact cloverleaf as material includes the steps of crushing material, cracking wall via adding hydrolase and mechanically, alkali extracting and acid precipitating, centrifugally separating, and leaching to decolorize and eliminate bad smell. The prepared soluble cloverleaf protein has high water solubility, coarse protein content near 80 % and comprehensive and balanced amino acid content, and is excellent functional food protein material.
Description
Technical field
A kind of preparation method of soluble heart trefoil protein, relating to a kind of is raw material with the briquetting cloverleaf, by enzymolysis broken wall and mechanical breaking-wall method, it is heavy that alkali is proposed acid, centrifugation, and raw meat is removed in decolouring again, and obtain the method for the edible alfalfa leaf protein of solubility, belong to field of vegetable protein producing technology.
Background technology
Leaf protein is to be raw material with fresh dark green plant stem-leaf, Separation of Proteins and concentrate drying in pressing extracting juice, juice and the protein concentrates (leaf protein concentration is called for short LPC) for preparing.Leaf protein because of have wide material sources, nutritious, do not contain the animality cholesterol, have and prevent and cure diseases, health care is anti-ageing, different physiological roles characteristics such as improve the health and receiving much concern, being thought a kind of high-quality food by FAO, is a kind of novel protein resource that high exploitation is worth that has.
Clover is a kind of good leguminous forage, be described as " king of herbage ", its adaptability is strong, wide in variety, cultivation history is grown, have a very wide distribution, have productivity stable, utilize a year limit for length, be of high nutritive value, using value such as good palatability, the livestock and poultry effect of feeding are good.Because the protein content in the clover is abundant, so be that the leaf protein of raw material is called alfalfa leaf protein with the clover.Leaf protein contains 18 seed amino acids, comprising the amino acid of 8 kinds of needed by human, and its proportion of composing balance, adult's amino acid pattern of recommending with FAO (Food and Agriculture Organization of the United Nation) conforms to substantially, and particularly lysine content is higher.
But all there are a lot of deficiencies in the alfalfa leaf protein of preparation at present.At first be that content is not high, generally have only 50%～60%; Next is that extracting method mostly is the heating flocculence greatly, and protein generation sex change is poorly soluble, and its purposes is restricted; Be that product does not have decolouring to remove raw meat once more, color and luster is dark green, and careless fishy smell is heavy, has also limited consumption and purposes in food.It is therefore domestic that it is mainly used in feed at present.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of soluble heart trefoil protein.Soluble heart trefoil protein matter has well water-soluble, and through the decolouring remove raw meat after, can in the numerous food system, be more widely used.
Technical scheme of the present invention: with the briquetting cloverleaf is raw material, through pulverizing, by adding water enzymolysis broken wall, mechanical breaking-wall method, alkali is carried, acid is heavy, and centrifugation is soaked the dike decolouring again and removed raw meat, obtain the edible alfalfa leaf protein matter of solubility, the process conditions of extracting are: the briquetting cloverleaf was pulverized 40 orders, and amount of water is 6～8 times of raw material cloverleaf in mass, adjust pH to 4.5, add cellulase, enzyme concentration is 2%～3% (w/w) of reaction system gross mass, 45 ℃ of enzyme digestion reaction temperature, 4～6 hours reaction time; Adjust pH to 7.8 was pulled an oar 3 minutes with tissue mashing machine again, soaking and stirring 30 minutes, and extruding is squeezed the juice and was got juice in 3 minutes; Adjust pH to 4.0 again, flocculated after 5 minutes on 3000 rev/mins of centrifuges centrifugal 10～15 minutes sediment; Removing raw meat, 95% ethanol consumption with 95% ethanol lixiviate decolouring again is 10～13 times that gained precipitates quality of material, 40 ℃～50 ℃ of extraction temperatures, extraction time 5～7 hours; Again with 1500 rev/mins centrifugal 3～5 minutes sediment; Freeze drying obtains the soluble heart trefoil protein product again.
Beneficial effect of the present invention: the prepared soluble heart trefoil protein matter of the present invention not only crude protein content is higher than common preparation method, and have well water-soluble, color and luster is light, several no careless fishy smell, amino acid balance, particularly lysine content be up to 4.83g/100g, and very easily absorbed by human consumption, therefore can be used as the sick children's of the old and the weak protide healthy food primary raw material, have broad application prospects.
The specific embodiment
Embodiment 1
Cloverleaf was pulverized 40 orders, and amount of water is material: water=1: 6, adjust pH to 4.5, enzyme are cellulase, and enzyme concentration is 2.5% (w/w), 45 ℃ of reaction temperatures, 4.5 hours reaction time; Adjust pH to 7.8 was pulled an oar 3 minutes with tissue mashing machine again, soaking and stirring 30 minutes, and extruding is squeezed the juice and was got juice in 3 minutes; Adjust pH to 4.0 again, flocculated after 5 minutes on 3000 rev/mins of centrifuges centrifugal 12 minutes sediment; Remove raw meat with 95% ethanol lixiviate decolouring again, the ethanol consumption is material: ethanol=1: 11,42 ℃ of extraction temperatures, extraction time 6 hours; Again with 1500 rev/mins centrifugal 4.5 minutes sediment; Freeze drying obtains the soluble heart trefoil protein product again, and crude protein content is 78.8%.
Embodiment 2
Cloverleaf was pulverized 40 orders, and amount of water is material: water=1: 7, adjust pH to 4.5, enzyme are cellulase, and enzyme concentration is 3.0% (w/w), 45 ℃ of reaction temperatures, 5 hours reaction time; Adjust pH to 7.8 was pulled an oar 3 minutes with tissue mashing machine again, soaking and stirring 30 minutes, and extruding is squeezed the juice and was got juice in 3 minutes; Adjust pH to 4.0 again, flocculated after 5 minutes on 3000 rev/mins of centrifuges centrifugal 12 minutes sediment; Remove raw meat with 95% ethanol decolorization again, the ethanol consumption is material: ethanol=1: 12,50 ℃ of extraction temperatures, extraction time 5 hours; Again with 1500 rev/mins centrifugal 3.5 minutes sediment; Freeze drying obtains the soluble heart trefoil protein product again, and crude protein content is 79.2%.
Claims (1)
1, a kind of extracting method of soluble heart trefoil protein, it is characterized in that with the briquetting cloverleaf be raw material, through pulverizing, by adding water enzymolysis broken wall, mechanical breaking-wall method, alkali is carried, and acid is heavy, centrifugation, soak the dike decolouring again and remove raw meat, obtain the edible alfalfa leaf protein matter of solubility, the process conditions of extraction are: the briquetting cloverleaf was pulverized 40 orders, amount of water is 6～8 times of raw material cloverleaf in mass, adjust pH to 4.5 adds cellulase, and enzyme concentration is 2%～3% of a reaction system gross mass, 45 ℃ of enzyme digestion reaction temperature, 4～6 hours reaction time; Adjust pH to 7.8 was pulled an oar 3 minutes with tissue mashing machine again, soaking and stirring 30 minutes, and extruding is squeezed the juice and was got juice in 3 minutes; Adjust pH to 4.0 again, flocculated after 5 minutes on 3000 rev/mins of centrifuges centrifugal 10～15 minutes sediment; Removing raw meat, 95% ethanol consumption with 95% ethanol lixiviate decolouring again is 10～13 times that gained precipitates quality of material, 40 ℃～50 ℃ of extraction temperatures, extraction time 5～7 hours; Again with 1500 rev/mins centrifugal 3～5 minutes sediment; Freeze drying obtains the soluble heart trefoil protein product again.
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| CN 200710023257 CN101077118A (en) | 2007-06-12 | 2007-06-12 | Method for preparing soluble heart trefoil protein |
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| CN 200710023257 CN101077118A (en) | 2007-06-12 | 2007-06-12 | Method for preparing soluble heart trefoil protein |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422504B (en) * | 2008-09-17 | 2010-12-08 | 姚常虹 | Preparation method of medicine red clover raw material for extracting isoflavone |
| CN101912029A (en) * | 2010-08-11 | 2010-12-15 | 天津商业大学 | Method for preparing grain amaranth high protein powder by double enzyme process |
| CN101984839A (en) * | 2010-10-18 | 2011-03-16 | 湖北省农科院中药材研究所 | Method for preparing pueraria lobata protein |
| WO2012150421A1 (en) * | 2011-05-04 | 2012-11-08 | Valagro Carbone Renouvelable Poitou-Charentes | Method for obtaining an alfalfa protein extract and reusable co-products |
| CN103205230A (en) * | 2013-05-08 | 2013-07-17 | 南京林业大学 | Preparation method for preparing biomass adhesive by utilizing alfalfa meal |
| CN103387599A (en) * | 2013-08-08 | 2013-11-13 | 河北考力森生物科技有限公司 | Technology for extracting protein of folium mori |
| CN103689608A (en) * | 2013-12-31 | 2014-04-02 | 大连民族学院 | Nutritional composition containing alfalfa leaf protein and preparation method of nutritional composition |
| CN104195204A (en) * | 2014-07-30 | 2014-12-10 | 内蒙古天奇生物科技有限公司 | Method for preparing alfalfa polypeptide |
| CN105018558A (en) * | 2015-07-03 | 2015-11-04 | 浙江黛君生物医药科技有限公司 | Clover multi-target combined production extraction separation method |
| CN106472963A (en) * | 2016-09-29 | 2017-03-08 | 齐齐哈尔大学 | A kind of discoloration method of alfalfa leaf protein anti-oxidation peptide |
| CN109198156A (en) * | 2017-06-30 | 2019-01-15 | 安琪酵母股份有限公司 | Yeast protein and its preparation method and application |
| CN109554413A (en) * | 2018-12-24 | 2019-04-02 | 蔡罗章 | A kind of non-edible section amino acid-extraction of clover |
| CN109777846A (en) * | 2018-12-24 | 2019-05-21 | 蔡罗章 | A kind of preparation method for extracting amino acid from clover |
| CN110041188A (en) * | 2019-05-22 | 2019-07-23 | 东华理工大学 | A method of extracting dihydrochalcone and brown pigment simultaneously from Pasania cuspidata leaf |
| CN110218238A (en) * | 2019-06-12 | 2019-09-10 | 淮阴师范学院 | Duckweed protein extract and its extracting method |
| CN114027388A (en) * | 2021-11-25 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content baicalin |
| CN114027387A (en) * | 2021-11-24 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content liquorice protein |
| CN117417406A (en) * | 2023-10-27 | 2024-01-19 | 江南大学 | Extraction method of radish leaf protein |
-
2007
- 2007-06-12 CN CN 200710023257 patent/CN101077118A/en active Pending
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422504B (en) * | 2008-09-17 | 2010-12-08 | 姚常虹 | Preparation method of medicine red clover raw material for extracting isoflavone |
| CN101912029A (en) * | 2010-08-11 | 2010-12-15 | 天津商业大学 | Method for preparing grain amaranth high protein powder by double enzyme process |
| CN101984839A (en) * | 2010-10-18 | 2011-03-16 | 湖北省农科院中药材研究所 | Method for preparing pueraria lobata protein |
| CN101984839B (en) * | 2010-10-18 | 2013-06-05 | 湖北省农科院中药材研究所 | Method for preparing pueraria lobata protein |
| WO2012150421A1 (en) * | 2011-05-04 | 2012-11-08 | Valagro Carbone Renouvelable Poitou-Charentes | Method for obtaining an alfalfa protein extract and reusable co-products |
| FR2974817A1 (en) * | 2011-05-04 | 2012-11-09 | Valagro Carbone Renouvelable Poitou Charentes | PROCESS FOR OBTAINING PROTEIN EXTRACT FROM LUZERNE AND VALORISABLE CO-PRODUCTS |
| CN103205230A (en) * | 2013-05-08 | 2013-07-17 | 南京林业大学 | Preparation method for preparing biomass adhesive by utilizing alfalfa meal |
| CN103387599B (en) * | 2013-08-08 | 2015-06-24 | 河北考力森生物科技有限公司 | Technology for extracting protein of folium mori |
| CN103387599A (en) * | 2013-08-08 | 2013-11-13 | 河北考力森生物科技有限公司 | Technology for extracting protein of folium mori |
| CN103689608A (en) * | 2013-12-31 | 2014-04-02 | 大连民族学院 | Nutritional composition containing alfalfa leaf protein and preparation method of nutritional composition |
| CN104195204A (en) * | 2014-07-30 | 2014-12-10 | 内蒙古天奇生物科技有限公司 | Method for preparing alfalfa polypeptide |
| CN105018558A (en) * | 2015-07-03 | 2015-11-04 | 浙江黛君生物医药科技有限公司 | Clover multi-target combined production extraction separation method |
| CN105018558B (en) * | 2015-07-03 | 2019-04-26 | 浙江黛君生物医药科技有限公司 | A kind of clover multiple target coproduction extraction separation method |
| CN106472963A (en) * | 2016-09-29 | 2017-03-08 | 齐齐哈尔大学 | A kind of discoloration method of alfalfa leaf protein anti-oxidation peptide |
| CN109198156B (en) * | 2017-06-30 | 2022-03-08 | 安琪纽特股份有限公司 | Yeast protein and preparation method and application thereof |
| CN109198156A (en) * | 2017-06-30 | 2019-01-15 | 安琪酵母股份有限公司 | Yeast protein and its preparation method and application |
| CN109554413A (en) * | 2018-12-24 | 2019-04-02 | 蔡罗章 | A kind of non-edible section amino acid-extraction of clover |
| CN109777846A (en) * | 2018-12-24 | 2019-05-21 | 蔡罗章 | A kind of preparation method for extracting amino acid from clover |
| CN110041188A (en) * | 2019-05-22 | 2019-07-23 | 东华理工大学 | A method of extracting dihydrochalcone and brown pigment simultaneously from Pasania cuspidata leaf |
| CN110218238A (en) * | 2019-06-12 | 2019-09-10 | 淮阴师范学院 | Duckweed protein extract and its extracting method |
| CN114027387A (en) * | 2021-11-24 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content liquorice protein |
| CN114027388A (en) * | 2021-11-25 | 2022-02-11 | 洛阳蓝斯利科技有限公司 | Preparation method of high-content baicalin |
| CN117417406A (en) * | 2023-10-27 | 2024-01-19 | 江南大学 | Extraction method of radish leaf protein |
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Open date: 20071128 |