CN104611402A - Preparation method for azolla leaf protein - Google Patents
Preparation method for azolla leaf protein Download PDFInfo
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- CN104611402A CN104611402A CN201510031961.3A CN201510031961A CN104611402A CN 104611402 A CN104611402 A CN 104611402A CN 201510031961 A CN201510031961 A CN 201510031961A CN 104611402 A CN104611402 A CN 104611402A
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- red duckweed
- leaf protein
- supernatant liquor
- protein
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Abstract
The invention provides a preparation method for azolla leaf protein and belongs to the field of food processing. Processes of enzymolysis, filtration, heating, fermentation, membrane separation, decolorization, vacuum freeze-drying and the like are adopted to solve problems in extraction and decolorization of the azolla leaf protein and produce the white grass-smell-free azolla leaf protein.
Description
Technical field
The invention belongs to food processing field, particularly relate to a kind of red duckweed leaf protein preparation method.
Technical background
Leaf protein (leaf protein coneentration, LPC) is with fresh dark green plant stem-leaf for raw material, the protein concentrates prepared through pressure extracting juice, juice protein separation and concentrate drying.Large quantity research chart bright leaf protein nutritive value is very abundant, leaf protein is containing crude protein 50%-65%, crude fat 15%-30%, ash content 0.5%-1.5%, carbohydrate 5%-20%, robust fibre 0.5%-11.5% (relatively wide numerical range is due to the difference between plant greenery and treatment process).Containing 18 seed amino acids in leaf protein protein, and wherein the necessary 8 kinds of essential amino acids content of human body are enriched.The amino acid classes of leaf protein complete and proportion of composing balance, the adult's aminoacid pattern recommended with Food and Argriculture OrganizationFAO conforms to substantially.Particularly lysine content is wherein abundanter, and utilization ratio is 82%, and this is the crowd of staple food with cereal for China, and edible leaf protein can supplement the deficiency of the first limiting amino acid-Methionin.Containing abundant carotene in leaf protein, containing carotene 1.0-1.3mg in every gram of leaf protein.Carotene not only can be used as natural pigment, simultaneously it or provitamin A, and it can be transformed into VA in mucous membrane of small intestine, improves significantly to the symptom such as organism metabolic disorder, growth retardation caused by VA shortage.Lutein content in leaf protein is up to 1100-1500mg.Xenthophylls is a kind of stable natural pigment, and it is not only the fabulous source of bird yolk, fat and skin pigment, and the meat of the cooking can also be made to present yellow, all welcome in a lot of country.Cholesterol in leaf protein contained by animal protein-free, and the content that can reduce Blood Cholesterol.Satoh etc. to feed mouse with the spinacin extracted from the leaf of spinach, find that its absorption that can suppress cholesterol increases cholesterol and bile acid excretion amount thus reduces cholesterol levels.At present in developed country, the cardiovascular morbidity caused owing to absorbing too much saturated fatty acid and cholesterol etc. in diet is high, in its diet, therefore increases vegetable-protein ratio go with the tide of historical development.
At present, developing country is all faced with protein crisis, and according to statistics, China is every year by short 2000t protein.The exploitation of new protein resource are current urgent problems.Leaf protein abundant raw material, be of high nutritive value, eating effect is good, high financial profit, is a kind of novel protein resource with value of exploiting and utilizing.
At present, both at home and abroad the extraction research of leaf protein is mainly concentrated on the plants such as clover, mulberry leaf, trifolium.And the research of extracting leaf protein from aquatic herbage is few.Red duckweed, as a kind of aquatic fern herbage, mainly concentrates on cultivation, as the aspect such as silage and ecological purification to its research at present.And economic use value does not also carry out deep exploitation, especially few to the research report that the extraction of leaf protein is wherein domestic.Red duckweed has very strong nitrogen fixing capacity, and in extra dry red wine duckweed, crude protein content is 17.4%, and fresh duckweed is 1.5% containing crude protein, and every mu of water surface produces thin red duckweed 2.5 ten thousand kg per year, and protein content is 375kg, and its protein content is equivalent to 20 mu of ground soy bean protein contents.Therefore, from red duckweed, leaf protein is extracted significant.
Summary of the invention
An object of the present invention is to provide a kind of red duckweed leaf protein preparation method.The present invention adopts the processes colors such as enzymolysis, filtration, heating, fermentation, membrane sepn, decolouring, vacuum lyophilization for white, without careless fishy smell, and natural free of contamination red duckweed leaf protein powder.
The technical solution used in the present invention is as follows:
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3-5, with hollander by red duckweed making beating 3-5min, then adding (red duckweed slurry quality 0.1-0.3%) proteolytic enzyme fully stirs after rear standing 1-2 h carries out enzymolysis, crosses leaching supernatant liquor for subsequent use with 800-2000 mesh filter screen.
(2) heat, ferment: (1) supernatant liquor of getting is put into rapidly after boiling water is heated to 40-45 DEG C and add bulgaricus ccm ((1) gets supernatant liquor quality 0.01-0.03%), thermophilus streptococcus ((1) gets supernatant liquor quality 0.01-0.03%), in confined conditions fermentation 36-48 hour.
(3) membrane sepn: the red duckweed liquid 10000-50000 dalton membrane ultrafiltration that will ferment through (2), separating whey solution and protein solution.(4) absorption, oxydasis decolouring: activity charcoal powder absorption 5-10 min will be put in (3) isolated protein solution, then filter and activity charcoal powder is removed, then add (protein solution quality 0.3-0.5%) lipoxygenase and fully stir rear standing 1-2 h and carry out enzymolysis.
(5) dry: to adopt vacuum lyophilization to obtain white red duckweed leaf protein powder the red duckweed leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8-9 mm, pre-freezing temperature-35--40 DEG C, pre-freeze time 2-2.5 h, temperature of heating plate 45-50 DEG C during distillation, dryness storehouse operating pressure 22-26Pa, temperature of heating plate 60-65 DEG C during parsing.
(6) pack: weigh by every pouch 5g, vacuum packaging.
Protease content in step (1) is the 0.1-0.3% of red duckweed slurry quality.
Bulgaricus ccm content is got supernatant liquor quality 0.01-0.03% by getting step (1) supernatant liquor quality 0.01-0.03%, thermophilus bacterial content by step (1) in step (2).
In step (4), lipoxygenase content is the 0.3-0.5% of protein solution quality.
Advantage of the present invention: the present invention with red duckweed for raw material warp → making beating → enzymolysis → filtration → heating → fermentation → membrane sepn → absorption → oxydasis decolouring → vacuum lyophilization → packaging → storage of weighing, the extraction yield of the red duckweed leaf protein obtained is 33.28%, red duckweed leaf protein yield is 1.34%, the color of red duckweed leaf protein is white, without careless fishy smell, protein mass mark is 56.68%.Aminoacids content in red duckweed leaf protein, in red duckweed leaf protein, amino acid classes is comparatively complete, wherein indispensable amino acid 7 kinds, non-essential amino acid 10 kinds, total amino acid content is 41.38%, and essential amino acids content accounts for 42.18% of total amino acid content, and the ratio of indispensable amino acid and non-essential amino acid content is 0.72, respectively higher than 40% and 0.6 of WHO/FAO standard regulation, red duckweed is that a kind of extraordinary leaf protein processes raw material.
Embodiment
Embodiment 1
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3, with hollander by red duckweed making beating 3min, then adds (red duckweed slurry quality 0.1%) proteolytic enzyme and fully stirs after rear standing 1h carries out enzymolysis, cross leaching supernatant liquor for subsequent use with 800 mesh filter screens.
(2) heat, fermentation: (1) supernatant liquor of getting is put into rapidly and after boiling water is heated to 40 DEG C, to add bulgaricus ccm ((1) gets supernatant liquor quality 0.01%), thermophilus streptococcus ((1) gets supernatant liquor quality 0.01%), fermentation in confined conditions 36 hours.
(3) membrane sepn: red duckweed liquid 10000 dalton's membrane ultrafiltration that will ferment through (2), separating whey solution and protein solution.(4) absorption, oxydasis decolouring: activity charcoal powder will be put in (3) isolated protein solution and adsorb 5 min, then filter by activity charcoal powder remove, then add (protein solution quality 0.3%) lipoxygenase fully stir rear leave standstill 1 h carry out enzymolysis.
(5) dry: to adopt vacuum lyophilization to obtain white red duckweed leaf protein powder the red duckweed leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8 mm, pre-freezing temperature-35 DEG C, pre-freeze time 2h, temperature of heating plate 45 DEG C during distillation, dryness storehouse operating pressure 22Pa, temperature of heating plate 60 DEG C during parsing.
(6) pack: weigh by every pouch 5g, vacuum packaging.
Embodiment 2
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:5, with hollander by red duckweed making beating 5min, then adds (red duckweed slurry quality 0.3%) proteolytic enzyme and fully stirs and leave standstill after 2 h carry out enzymolysis afterwards, cross leaching supernatant liquor for subsequent use with 2000 mesh filter screens.
(2) heat, fermentation: (1) supernatant liquor of getting is put into rapidly and after boiling water is heated to 45 DEG C, to add bulgaricus ccm ((1) gets supernatant liquor quality 0.03%), thermophilus streptococcus ((1) gets supernatant liquor quality 0.03%), fermentation in confined conditions 48 hours.
(3) membrane sepn: red duckweed liquid 50000 dalton's membrane ultrafiltration that will ferment through (2), separating whey solution and protein solution.(4) absorption, oxydasis decolouring: activity charcoal powder will be put in (3) isolated protein solution and adsorb 10 min, then filter and activity charcoal powder is removed, then add (protein solution quality 0.5%) lipoxygenase and fully stir rear quiet 1-2 h and carry out enzymolysis.
(5) dry: to adopt vacuum lyophilization to obtain white red duckweed leaf protein powder the red duckweed leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 9 mm, pre-freezing temperature-40 DEG C, pre-freeze time 2.5 h, temperature of heating plate 50 DEG C during distillation, dryness storehouse operating pressure 26Pa, temperature of heating plate 65 DEG C during parsing.
(6) pack: weigh by every pouch 5g, vacuum packaging.
Embodiment 3
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:4, with hollander by red duckweed making beating 4min, then add (red duckweed slurry quality 0.2%) proteolytic enzyme fully to stir and leave standstill after 1.5 h carry out enzymolysis afterwards, cross leaching supernatant liquor with 1500 mesh filter screens for subsequent use.
(2) heat, fermentation: (1) supernatant liquor of getting is put into rapidly and after boiling water is heated to 42 DEG C, to add bulgaricus ccm ((1) gets supernatant liquor quality 0.02%), thermophilus streptococcus ((1) gets supernatant liquor quality 0.02%), fermentation in confined conditions 40 hours.
(3) membrane sepn: red duckweed liquid 30000 dalton's membrane ultrafiltration that will ferment through (2), separating whey solution and protein solution.
(4) absorption, oxydasis decolouring: activity charcoal powder absorption 5-10 min will be put in (3) isolated protein solution, then filter by activity charcoal powder remove, then add (protein solution quality 0.4%) lipoxygenase fully stir rear leave standstill 1.5 h carry out enzymolysis.
(5) dry: to adopt vacuum lyophilization to obtain white red duckweed leaf protein powder the red duckweed leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8 mm, pre-freezing temperature-37 DEG C, pre-freeze time 2 h, temperature of heating plate 47 DEG C during distillation, dryness storehouse operating pressure 24Pa, temperature of heating plate 62 DEG C during parsing.
(6) pack: weigh by every pouch 5g, vacuum packaging.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (6)
1. a red duckweed leaf protein preparation method, is characterized in that: obtain red duckweed leaf protein by enzymolysis, filtration, heating, fermentation, membrane sepn, decolouring, vacuum lyophilization.
2. one according to claim 1 red duckweed leaf protein preparation method, is characterized in that: described method comprises step:
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3-5, with hollander by red duckweed making beating 3-5min, then adds proteolytic enzyme and fully stirs after rear standing 1-2 h carries out enzymolysis, cross leaching supernatant liquor for subsequent use with 800-2000 mesh filter screen;
(2) heat, fermentation: (1) supernatant liquor of getting is put into rapidly and to add bulgaricus ccm, thermophilus streptococcus after boiling water is heated to 40-45 DEG C and to ferment in confined conditions 36-48 hour;
(3) membrane sepn: the red duckweed liquid 10000-50000 dalton membrane ultrafiltration that will ferment through (2), separating whey solution and protein solution;
(4) adsorb, oxydasis decolouring: activity charcoal powder absorption 5-10 min will be put in (3) isolated protein solution, then filter and activity charcoal powder is removed, then add lipoxygenase and fully stir rear standing 1-2 h and carry out enzymolysis;
(5) dry: to adopt vacuum lyophilization to obtain white red duckweed leaf protein powder the red duckweed leaf protein solution after (4) absorption, oxydasis decolouring;
(6) pack: weigh by every pouch 5g, vacuum packaging.
3. one according to claim 2 red duckweed leaf protein preparation method, is characterized in that: the protease content in step (1) is the 0.1-0.3% of red duckweed slurry quality.
4. one according to claim 2 red duckweed leaf protein preparation method, is characterized in that: bulgaricus ccm content is got supernatant liquor quality 0.01-0.03% by getting step (1) supernatant liquor quality 0.01-0.03%, thermophilus bacterial content by step (1) in step (2).
5. one according to claim 2 red duckweed leaf protein preparation method, is characterized in that: in step (4), lipoxygenase content is the 0.3-0.5% of protein solution quality.
6. one according to claim 2 red duckweed leaf protein preparation method, it is characterized in that: step (5) parameters of freeze-drying process: material thickness 8-9 mm, pre-freezing temperature-35--40 DEG C, pre-freeze time 2-2.5 h, temperature of heating plate 45-50 DEG C during distillation, dryness storehouse operating pressure 22-26Pa, temperature of heating plate 60-65 DEG C during parsing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510031961.3A CN104611402A (en) | 2015-01-22 | 2015-01-22 | Preparation method for azolla leaf protein |
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| CN201510031961.3A CN104611402A (en) | 2015-01-22 | 2015-01-22 | Preparation method for azolla leaf protein |
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| CN104611402A true CN104611402A (en) | 2015-05-13 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379942A (en) * | 2015-11-19 | 2016-03-09 | 重庆三零三科技有限公司 | Extraction method of proteins of cucumber leaves |
| CN105410325A (en) * | 2015-11-23 | 2016-03-23 | 重庆三零三科技有限公司 | Preparation method of lettuce leaf protein |
| CN108347967A (en) * | 2015-09-10 | 2018-07-31 | 帕拉贝尔有限公司 | Method and system for processing high concentration protein product from micro- crop and its composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450364A (en) * | 2010-10-25 | 2012-05-16 | 程庆 | Dealcoholic health protection tea |
-
2015
- 2015-01-22 CN CN201510031961.3A patent/CN104611402A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450364A (en) * | 2010-10-25 | 2012-05-16 | 程庆 | Dealcoholic health protection tea |
Non-Patent Citations (2)
| Title |
|---|
| 姜传京: "红萍叶蛋白提取及营养价值评价研究", 《中国优秀硕士学学位论文全文数据库（电子期刊）工程科技I辑》 * |
| 王璋: "《食品酶学》", 30 April 1990, 轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108347967A (en) * | 2015-09-10 | 2018-07-31 | 帕拉贝尔有限公司 | Method and system for processing high concentration protein product from micro- crop and its composition |
| CN105379942A (en) * | 2015-11-19 | 2016-03-09 | 重庆三零三科技有限公司 | Extraction method of proteins of cucumber leaves |
| CN105410325A (en) * | 2015-11-23 | 2016-03-23 | 重庆三零三科技有限公司 | Preparation method of lettuce leaf protein |
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Application publication date: 20150513 |
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