CN105379942A - Extraction method of proteins of cucumber leaves - Google Patents
Extraction method of proteins of cucumber leaves Download PDFInfo
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- CN105379942A CN105379942A CN201510798925.XA CN201510798925A CN105379942A CN 105379942 A CN105379942 A CN 105379942A CN 201510798925 A CN201510798925 A CN 201510798925A CN 105379942 A CN105379942 A CN 105379942A
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Abstract
The invention discloses an extraction method of proteins of cucumber leaves. The method comprises the following steps: performing enzymolysis, filtering, heating, fermentation, film separation, decoloring, vacuum freeze drying and the like on cucumber leaves serving as raw materials. The raw materials for preparing the proteins of the cucumber leaves are readily available, and the extraction technology is simple; the extracted leaf proteins can be applied to feeds, food, medicines and cosmetics.
Description
Technical field
The invention belongs to biological technical field, particularly relate to the extracting method of cucumber leaf albumen.
Background technology
Pumpkin is the common a kind of food of China, has the effect of reducing blood lipid.Cushaw leaf is the leaf of pumpkin (cucurbitamoschataDuch), is rich in protein, amino acid, mineral matter, also containing beta carotene, aldehydes matter and flavones.In Nigeria, cushaw leaf is very welcome vegetables, is often used to cook soup, also commonly uses and does herbtherapy disease.But China is also more rare to the utilization of cushaw leaf.
China's pumpkin output is in the trend increased year by year, and the cushaw leaf resource as pumpkin accessory substance is very abundant, but major part is abandoned in vain, if be exploited, prospect is quite wide.
The crude protein content of cushaw leaf can reach 46.3% of full leaf dry weight, higher than the protein content in full milk powder, broad bean, and close with soybean protein.Find cushaw leaf gal4 amino acid composition analysis, the amino acid content of needed by human enriches.In addition, cushaw leaf is rich in leucine, lysine, threonine and valine.Cushaw leaf mineral content is very abundant, and collocation is balanced, is desirable basic food.Mankind's fatigue is caused by the consumption of potassium and the accumulation of sodium, and cushaw leaf potassium content is up to 710.8mg/100g, is 2.5 times of banana, 2 times of wheat straw.So cushaw leaf belongs to hyperkalemia hyponatremia plant, often edible can antifatigue, and for angiocardiopathies such as preventing hypertensions, there is positive effect.In cushaw leaf, calcium, magnesium and Zn content are abundant, can improve mankind's appetite, develop immunitypty.The beta carotene be rich in cushaw leaf, vitamin C, aldehydes matter and flavones then can have the effects such as antitumor, anti-oxidant.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide the extracting method of cucumber leaf albumen, it is characterized in that, realized by following steps:
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3-5, the cucumber leaf making beating 3-5min drained will be cleaned with beater, then adding (cucumber leaf slurry quality 0.1-0.3%) protease fully stirs after rear standing 1-2h carries out enzymolysis, crosses leaching supernatant for subsequent use with 800-2000 mesh filter screen.
(2) heat, ferment: (1) supernatant of getting is put into rapidly after boiling water is heated to 40-45 DEG C and add Bulgarian Lactobacillus ((1) gets supernatant quality 0.01-0.03%), streptococcus thermophilus ((1) gets supernatant quality 0.01-0.03%), in confined conditions fermentation 36-48 hour.
(3) UF membrane: the cucumber leaf liquid 10000-50000 dalton film ultrafiltration will fermented through (2), separating whey solution and protein solution.
(4) absorption, oxydasis decolouring: activated carbon powder absorption 5-10min will be put in (3) isolated protein solution, then filter and activated carbon powder is removed, then add (protein solution quality 0.3-0.5%) lipoxygenase and fully stir rear standing 1-2h and carry out enzymolysis.
(5) dry: to adopt vacuum freeze drying to obtain white cucumber leaf albumen powder the cucumber leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8-9mm, pre-freezing temperature-35--40 DEG C, pre-freeze time 2-2.5h, temperature of heating plate 45-50 DEG C during distillation, dryness storehouse operating pressure 22-26Pa, temperature of heating plate 60-65 DEG C during parsing.
Protease content in step (1) is the 0.1-0.3% of cucumber leaf slurry quality.
Bulgarian Lactobacillus content is got supernatant quality 0.01-0.03% by getting step (1) supernatant quality 0.01-0.03%, thermophilus bacterial content by step (1) in step (2).
In step (4), lipoxygenase content is the 0.3-0.5% of protein solution quality.
Advantage of the present invention: the present invention take cucumber leaf as raw material warp → making beating → enzymolysis → filtration → heating → fermentation → UF membrane → absorption → oxydasis decolouring → vacuum freeze drying, the recovery rate of the cucumber leaf albumen obtained is 50.32%, cucumber leaf albumen yield is 7.45%, the color of cucumber leaf albumen is white, without careless fishy smell, albumen quality mark is 63.24%.
Detailed description of the invention
Embodiment 1
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3, the cucumber leaf making beating 3min drained will be cleaned with beater, then adding (cucumber leaf slurry quality 0.1%) protease fully stirs after rear standing 1h carries out enzymolysis, crosses leaching supernatant for subsequent use with 800 mesh filter screens.
(2) heat, fermentation: (1) supernatant of getting is put into rapidly and after boiling water is heated to 40 DEG C, to add Bulgarian Lactobacillus ((1) gets supernatant quality 0.01%), streptococcus thermophilus ((1) gets supernatant quality 0.01%), fermentation in confined conditions 36 hours.
(3) UF membrane: the cucumber leaf liquid that will ferment through (2) 10000 dalton's film ultrafiltration, separating whey solution and protein solution.
(4) absorption, oxydasis decolouring: activated carbon powder absorption 5min will be put in (3) isolated protein solution, then filter and activated carbon powder is removed, then add (protein solution quality 0.3%) lipoxygenase and fully stir rear standing 1h and carry out enzymolysis.
(5) dry: to adopt vacuum freeze drying to obtain white cucumber leaf albumen powder the cucumber leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8mm, pre-freezing temperature-35 DEG C, pre-freeze time 2h, temperature of heating plate 45 DEG C during distillation, dryness storehouse operating pressure 22Pa, temperature of heating plate 60 DEG C during parsing.
Embodiment 2
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:5, the cucumber leaf making beating 5min drained will be cleaned with beater, then adding (cucumber leaf slurry quality 0.3%) protease fully stirs after rear standing 2h carries out enzymolysis, crosses leaching supernatant for subsequent use with 2000 mesh filter screens.
(2) heat, fermentation: (1) supernatant of getting is put into rapidly and after boiling water is heated to 45 DEG C, to add Bulgarian Lactobacillus ((1) gets supernatant quality 0.03%), streptococcus thermophilus ((1) gets supernatant quality 0.03%), fermentation in confined conditions 48 hours.
(3) UF membrane: the cucumber leaf liquid that will ferment through (2) 50000 dalton's film ultrafiltration, separating whey solution and protein solution.
(4) absorption, oxydasis decolouring: activated carbon powder absorption 10min will be put in (3) isolated protein solution, then filter and activated carbon powder is removed, then add (protein solution quality 0.5%) lipoxygenase and fully stir rear quiet 1-2h and carry out enzymolysis.
(5) dry: to adopt vacuum freeze drying to obtain white cucumber leaf albumen powder the cucumber leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 9mm, pre-freezing temperature-40 DEG C, pre-freeze time 2.5h, temperature of heating plate 50 DEG C during distillation, dryness storehouse operating pressure 26Pa, temperature of heating plate 65 DEG C during parsing.
Embodiment 3
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:4, the cucumber leaf making beating 4min drained will be cleaned with beater, then adding (cucumber leaf slurry quality 0.2%) protease fully stirs after rear standing 1.5h carries out enzymolysis, crosses leaching supernatant for subsequent use with 1500 mesh filter screens.
(2) heat, fermentation: (1) supernatant of getting is put into rapidly and after boiling water is heated to 42 DEG C, to add Bulgarian Lactobacillus ((1) gets supernatant quality 0.02%), streptococcus thermophilus ((1) gets supernatant quality 0.02%), fermentation in confined conditions 40 hours.
(3) UF membrane: the cucumber leaf liquid that will ferment through (2) 30000 dalton's film ultrafiltration, separating whey solution and protein solution.
(4) absorption, oxydasis decolouring: activated carbon powder absorption 5-10min will be put in (3) isolated protein solution, then filter and activated carbon powder is removed, then add (protein solution quality 0.4%) lipoxygenase and fully stir rear standing 1.5h and carry out enzymolysis.
(5) dry: to adopt vacuum freeze drying to obtain white cucumber leaf albumen powder the cucumber leaf protein solution after (4) absorption, oxydasis decolouring.Parameters of freeze-drying process: material thickness 8mm, pre-freezing temperature-37 DEG C, pre-freeze time 2h, temperature of heating plate 47 DEG C during distillation, dryness storehouse operating pressure 24Pa, temperature of heating plate 62 DEG C during parsing.
Above about specific descriptions of the present invention, be only not limited to the technical scheme that embodiment of the present invention rope describes for illustration of the present invention, those of ordinary skill in the art should be appreciated that and still can modify to the present invention or equivalent replacement.
Claims (5)
1. the extracting method of cucumber leaf albumen, is characterized in that described method with the leaf of cucumber for raw material, carries out according to the following steps:
(1) making beating, enzymolysis, filtration: adopt solid-liquid ratio 1:3-5, will clean the cucumber leaf making beating 3-5min that drain with beater, then add protease and fully stir after rear standing 1-2h carries out enzymolysis, cross leaching supernatant for subsequent use with 800-1000 mesh filter screen;
(2) heat, fermentation: (1) supernatant of getting is put into rapidly and to add Bulgarian Lactobacillus, streptococcus thermophilus after boiling water is heated to 40-45 DEG C and to ferment in confined conditions 36-48 hour;
(3) UF membrane: the cucumber leaf liquid 10000-50000 dalton film ultrafiltration will fermented through (2), separating whey solution and protein solution;
(4) adsorb, oxydasis decolouring: activated carbon powder absorption 5-10min will be put in (3) isolated protein solution, then filter and activated carbon powder is removed, then add lipoxygenase and fully stir rear standing 1-2h and carry out enzymolysis;
(5) dry: to adopt vacuum freeze drying to obtain white cucumber leaf albumen powder the cucumber leaf protein solution after (4) absorption, oxydasis decolouring.
2. the extracting method of cucumber leaf albumen according to claim 1, is characterized in that: the protease content in step (1) is the 0.1-0.3% of cucumber leaf slurry quality.
3. the extracting method of cucumber leaf albumen according to claim 1, is characterized in that: Bulgarian Lactobacillus content is got supernatant quality 0.01-0.03% by getting step (1) supernatant quality 0.01-0.03%, thermophilus bacterial content by step (1) in step (2).
4. the extracting method of cucumber leaf albumen according to claim 1, is characterized in that: in step (4), lipoxygenase content is the 0.3-0.5% of protein solution quality.
5. the extracting method of cucumber leaf albumen according to claim 1, it is characterized in that: step (5) parameters of freeze-drying process: material thickness 8-9mm, pre-freezing temperature-35--40 DEG C, pre-freeze time 2-2.5h, temperature of heating plate 45-50 DEG C during distillation, dryness storehouse operating pressure 22-26Pa, temperature of heating plate 60-65 DEG C during parsing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510798925.XA CN105379942A (en) | 2015-11-19 | 2015-11-19 | Extraction method of proteins of cucumber leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510798925.XA CN105379942A (en) | 2015-11-19 | 2015-11-19 | Extraction method of proteins of cucumber leaves |
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| CN105379942A true CN105379942A (en) | 2016-03-09 |
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| CN201510798925.XA Withdrawn CN105379942A (en) | 2015-11-19 | 2015-11-19 | Extraction method of proteins of cucumber leaves |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450394A (en) * | 2010-10-25 | 2012-05-16 | 郝明然 | Extraction method of grass leaf protein |
| CN104611402A (en) * | 2015-01-22 | 2015-05-13 | 福建省农业科学院农业生态研究所 | Preparation method for azolla leaf protein |
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2015
- 2015-11-19 CN CN201510798925.XA patent/CN105379942A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450394A (en) * | 2010-10-25 | 2012-05-16 | 郝明然 | Extraction method of grass leaf protein |
| CN104611402A (en) * | 2015-01-22 | 2015-05-13 | 福建省农业科学院农业生态研究所 | Preparation method for azolla leaf protein |
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Application publication date: 20160309 |
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