CN106609293A - Tomato leaf protein extraction method - Google Patents
Tomato leaf protein extraction method Download PDFInfo
- Publication number
- CN106609293A CN106609293A CN201510693797.2A CN201510693797A CN106609293A CN 106609293 A CN106609293 A CN 106609293A CN 201510693797 A CN201510693797 A CN 201510693797A CN 106609293 A CN106609293 A CN 106609293A
- Authority
- CN
- China
- Prior art keywords
- leaf
- tomato leaf
- fructus lycopersici
- lycopersici esculenti
- tomato
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a tomato leaf protein extraction method. The tomato leaf protein extraction method comprises the steps of taking tomato leaves as a raw material, and performing enzymolysis, filtering, heating, fermenting, film separating, de-coloring, vacuum freezing and drying and the like. By adoption of the method, protein components in the tomato leaves can be extracted completely, so that the tomato leaf protein extraction method is wide in development prospect; in addition, the raw material is easy to get and the extraction process is simple; and the extracted protection can be applied to feed, food, medicines and cosmetics.
Description
Technical field
The invention belongs to biological technical field, more particularly to the extracting method of Fructus Lycopersici esculenti leaf protein.
Background technology
At present, developing country all suffers from protein crisis, and China's protein is seriously in short supply.The exploitation of novel protein resource are current urgent problems.Leaf protein abundant raw material, be of high nutritive value, eating effect is good, high financial profit, is a kind of novel protein resource with value of exploiting and utilizing.
At present, the extraction research both at home and abroad to leaf protein is concentrated mainly on the plants such as Herba Medicaginiss, Folium Mori, Herba Trifolii Pratentis, and less to the domestic and international pertinent literature report of research of Fructus Lycopersici esculenti leaf protein.
Fructus Lycopersici esculenti calls Fructus Lycopersici esculenti, and Solanaceae Fructus Lycopersici esculenti platymiscium originates in South America, extensively cultivates in China north and south, wherein maximum with Xinjiang yield.It is well known that containing antioxidant contents such as vitamin in tamato fruit, applying has beauty anti-wrinkle effect in cosmetics.But the leaf of Fructus Lycopersici esculenti is not utilized by fully research and development, after annual Fruits of Tomatoes is plucked, tomato plant all will be discarded, further not developed processing and utilization.In fact, equally having a good application prospect, but not yet having pertinent literature to report at present containing bioactive substances such as rich in protein, aminoacid, vitamin, flavone in tomato leaf.
Tomato leaf has very strong nitrogen fixing capacity, and in dry tomato leaf, crude protein content is 52.19%, is a kind of rich protein-contg plant, therefore, leaf protein is extracted from tomato leaf significant.
The content of the invention
Present invention aims to the deficiencies in the prior art, there is provided the extracting method of Fructus Lycopersici esculenti leaf protein, it is characterised in that through the following steps that realize：
(1) beating, enzymolysis, filtration：Using solid-liquid ratio 1:3-5, will clean the tomato leaf beating 3-5min for draining with beater, be subsequently adding（Tomato leaf starches quality 0.1-0.3%）Protease is stood after being sufficiently stirred for after 1-2 h are digested,
Supernatant is filtered to take with 800-2000 mesh filter screens standby.
（2）Heating, fermentation：(1) taken supernatant is put into rapidly after 40-45 DEG C being heated in boiling water add Bulgarian Lactobacillus ((1) taken supernatant quality 0.01-0.03%), streptococcus thermophiluss ((1) taken supernatant quality 0.01-0.03%), 36-48 hours of fermenting in confined conditions.
（3）Membrance separation：By Jing（2）The tomato leaf liquid 10000-50000 dalton's membrane ultrafiltration for fermenting, separating whey solution and protein solution.
（4）Absorption, oxydasises decolourize：By Jing（3）Activated carbon powder absorption 5-10 min are put in the protein solution isolated, are then filtered and activated carbon powder is removed, added standing 1-2 h after (protein solution quality 0.3-0.5%) lipoxidase is sufficiently stirred for and digested.
（5）It is dried：By Jing（4）Tomato leaf protein solution after absorption, oxydasises decolourize is obtained white tomato leaf egg albumen powder using vacuum lyophilization.Parameters of freeze-drying process：Material thickness 8-9 mm, -35--40 DEG C of pre-freezing temperature, pre-freeze time 2-2.5h, 45-50 DEG C of temperature of heating plate during distillation, dryness storehouse operating pressure 22-26Pa, 60-65 DEG C of temperature of heating plate during parsing.
Step（1）In protease content be tomato leaf starch quality 0.1-0.3%.
Step（2）Middle Bulgarian Lactobacillus content takes supernatant quality 0.01-0.03% by step (1) by taking step (1) supernatant quality 0.01-0.03%, thermophilus bacterial content.
Step（4）0.3-0.5% of the middle lipoxidase content for protein solution quality.
Advantages of the present invention：The present invention is with tomato leaf as raw material Jing → beating → enzymolysis → filtration → heating → fermentation → membrance separation → absorption → oxydasises decolouring → vacuum lyophilization, the extraction ratio of the Fructus Lycopersici esculenti leaf protein for obtaining is 50.32%, Fructus Lycopersici esculenti leaf protein yield is 7.45%, the color of Fructus Lycopersici esculenti leaf protein is white, without careless fishy smell, albumen quality fraction is 63.24%.
Specific embodiment
Embodiment 1
(1) beating, enzymolysis, filtration：Using solid-liquid ratio 1:3, the tomato leaf beating 3min for draining will be cleaned with beater, be subsequently adding（Tomato leaf starches quality 0.1%）Protease is stood after being sufficiently stirred for after 1h digested,
Supernatant is filtered to take with 800 mesh filter screens standby.
（2）Heating, fermentation：(1) taken supernatant is put in boiling water rapidly and is heated to add Bulgarian Lactobacillus ((1) taken supernatant quality 0.01%), in confined conditions streptococcus thermophiluss ((1) taken supernatant quality 0.01%), fermentation 36 hours after 40 DEG C.
（3）Membrance separation：By Jing（2）The tomato leaf liquid for fermenting 10000 dalton's membrane ultrafiltration, separating whey solution and protein solution.
（4）Absorption, oxydasises decolourize：By Jing（3）Activated carbon powder is put in the protein solution isolated and adsorbs 5 min, then filtered and activated carbon powder is removed, add (protein solution quality 0.3%) lipoxidase and 1 h is stood after being sufficiently stirred for and is digested.
（5）It is dried：By Jing（4）Tomato leaf protein solution after absorption, oxydasises decolourize is obtained white tomato leaf egg albumen powder using vacuum lyophilization.Parameters of freeze-drying process：8 mm of material thickness, -35 DEG C of pre-freezing temperature, pre-freeze time 2h, 45 DEG C of temperature of heating plate during distillation, dryness storehouse operating pressure 22Pa, 60 DEG C of temperature of heating plate during parsing.
Embodiment 2
(1) beating, enzymolysis, filtration：Using solid-liquid ratio 1:5, the tomato leaf beating 5min for draining will be cleaned with beater, be subsequently adding（Tomato leaf starches quality 0.3%）Protease is stood after being sufficiently stirred for after 2 h are digested,
Supernatant is filtered to take with 2000 mesh filter screens standby.
（2）Heating, fermentation：(1) taken supernatant is put in boiling water rapidly and is heated to add Bulgarian Lactobacillus ((1) taken supernatant quality 0.03%), in confined conditions streptococcus thermophiluss ((1) taken supernatant quality 0.03%), fermentation 48 hours after 45 DEG C.
（3）Membrance separation：By Jing（2）The tomato leaf liquid for fermenting 50000 dalton's membrane ultrafiltration, separating whey solution and protein solution.
（4）Absorption, oxydasises decolourize：By Jing（3）Activated carbon powder is put in the protein solution isolated and adsorbs 10 min, then filtered and activated carbon powder is removed, added quiet 1-2 h after (protein solution quality 0.5%) lipoxidase is sufficiently stirred for and digested.
（5）It is dried：By Jing（4）Tomato leaf protein solution after absorption, oxydasises decolourize is obtained white tomato leaf egg albumen powder using vacuum lyophilization.Parameters of freeze-drying process：9 mm of material thickness, -40 DEG C of pre-freezing temperature, 2.5 h of pre-freeze time, 50 DEG C of temperature of heating plate during distillation, dryness storehouse operating pressure 26Pa, 65 DEG C of temperature of heating plate during parsing.
Embodiment 3
(1) beating, enzymolysis, filtration：Using solid-liquid ratio 1:4, the tomato leaf beating 4min for draining will be cleaned with beater, be subsequently adding（Tomato leaf starches quality 0.2%）Protease is stood after being sufficiently stirred for after 1.5 h are digested,
Supernatant is filtered to take with 1500 mesh filter screens standby.
（2）Heating, fermentation：(1) taken supernatant is put in boiling water rapidly and is heated to add Bulgarian Lactobacillus ((1) taken supernatant quality 0.02%), in confined conditions streptococcus thermophiluss ((1) taken supernatant quality 0.02%), fermentation 40 hours after 42 DEG C.
（3）Membrance separation：By Jing（2）The tomato leaf liquid for fermenting 30000 dalton's membrane ultrafiltration, separating whey solution and protein solution.
（4）Absorption, oxydasises decolourize：By Jing（3）Activated carbon powder absorption 5-10min is put in the protein solution isolated, is then filtered and activated carbon powder is removed, add (protein solution quality 0.4%) lipoxidase and 1.5 h are stood after being sufficiently stirred for and is digested.
（5）It is dried：By Jing（4）Tomato leaf protein solution after absorption, oxydasises decolourize is obtained white tomato leaf egg albumen powder using vacuum lyophilization.Parameters of freeze-drying process：8 mm of material thickness, -37 DEG C of pre-freezing temperature, 2 h of pre-freeze time, 47 DEG C of temperature of heating plate during distillation, dryness storehouse operating pressure 24Pa, 62 DEG C of temperature of heating plate during parsing.
Above with respect to the specific descriptions of the present invention, it is merely to illustrate the present invention and is not limited to the technical scheme of embodiment of the present invention rope description, it will be understood by those within the art that, still the present invention can be modified or equivalent.
Claims (5)
1. the extracting method of Fructus Lycopersici esculenti leaf protein, it is characterised in that described method is carried out according to the following steps with the leaf of Fructus Lycopersici esculenti as raw material：
(1) beating, enzymolysis, filtration：Using solid-liquid ratio 1:3-5, will clean the tomato leaf beating 3-5min that drain with beater, be subsequently adding after protease is sufficiently stirred for and stand after 1-2 h are digested, filter to take supernatant with 800-1000 mesh filter screens standby；
（2）Heating, fermentation：(1) taken supernatant is put into rapidly after 40-45 DEG C being heated in boiling water add Bulgarian Lactobacillus, streptococcus thermophiluss to ferment in confined conditions 36-48 hours；
（3）Membrance separation：By Jing（2）The tomato leaf liquid 10000-50000 dalton's membrane ultrafiltration for fermenting, separating whey solution and protein solution；
（4）Absorption, oxydasises decolourize：By Jing（3）Activated carbon powder absorption 5-10 min are put in the protein solution isolated, are then filtered and activated carbon powder is removed, added standing 1-2 h after lipoxidase is sufficiently stirred for and digested；
（5）It is dried：By Jing（4）Tomato leaf protein solution after absorption, oxydasises decolourize is obtained white tomato leaf egg albumen powder using vacuum lyophilization.
2. the extracting method of the Fructus Lycopersici esculenti leaf protein according to claim 1, it is characterised in that：Step（1）In protease content be tomato leaf starch quality 0.1-0.3%.
3. the extracting method of the Fructus Lycopersici esculenti leaf protein according to claim 1, it is characterised in that：Step（2）Middle Bulgarian Lactobacillus content takes supernatant quality 0.01-0.03% by step (1) by taking step (1) supernatant quality 0.01-0.03%, thermophilus bacterial content.
4. the extracting method of the Fructus Lycopersici esculenti leaf protein according to claim 1, it is characterised in that：Step（4）0.3-0.5% of the middle lipoxidase content for protein solution quality.
5. the extracting method of the Fructus Lycopersici esculenti leaf protein according to claim 1, it is characterised in that：Step（5）Parameters of freeze-drying process：Material thickness 8-9 mm, -35--40 DEG C of pre-freezing temperature, pre-freeze time 2-2.5 h, 45-50 DEG C of temperature of heating plate during distillation, dryness storehouse operating pressure 22-26Pa, 60-65 DEG C of temperature of heating plate during parsing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510693797.2A CN106609293A (en) | 2015-10-24 | 2015-10-24 | Tomato leaf protein extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510693797.2A CN106609293A (en) | 2015-10-24 | 2015-10-24 | Tomato leaf protein extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106609293A true CN106609293A (en) | 2017-05-03 |
Family
ID=58612528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510693797.2A Pending CN106609293A (en) | 2015-10-24 | 2015-10-24 | Tomato leaf protein extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106609293A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616256A (en) * | 2020-06-15 | 2020-09-04 | 宁翠平 | Method for extracting leaf-eating vegetable protein |
-
2015
- 2015-10-24 CN CN201510693797.2A patent/CN106609293A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616256A (en) * | 2020-06-15 | 2020-09-04 | 宁翠平 | Method for extracting leaf-eating vegetable protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102318724B (en) | Production method of acid soybean peptide protein | |
| CN101191115A (en) | Honeysuckle fruit vinegar beverage | |
| CN104017098B (en) | A kind of method from mushroom waste mushroom stick extracting directly mushroom active polysaccharide | |
| CN104480168A (en) | Method for preparing chondroitin sulfate and co-producing collagen peptide and biological calcium by using fishbone | |
| CN109769991B (en) | Production process for enzymatic synthesis of polyester catechin by using exogenous enzyme | |
| CN106119326A (en) | The method extracting collagen protein polypeptide coproduction chrondroitin from cattle cartilage | |
| CN104177507A (en) | Preparation method of garlic polysaccharide | |
| CN104387488A (en) | Method for extracting mesona chinensis benth polysaccharide through microbial fermentation | |
| CN106832021A (en) | A kind of preparation method of Chinese cassia tree slag Thick many candies | |
| CN105543289A (en) | Oak acorn tannin extraction and starch thick mash fermentation coupling method | |
| CN105192663B (en) | A kind of preparation method of black tea essence | |
| CN105859675A (en) | Preparation method of high-purity anthocyanins by extracting from roselle | |
| CN108558815A (en) | A kind of highly effective extraction method of blueberry anthocyanin | |
| CN102071180A (en) | New process for preparing pineapple bromelain | |
| CN106892994A (en) | A kind of preparation method of blueberry residue Thick many candies | |
| CN106609293A (en) | Tomato leaf protein extraction method | |
| CN104531822A (en) | Purple sweet potato cyanidin efficient synthesis and extraction method | |
| CN104189087B (en) | A kind of method extracting total flavonoid functional materials from Folium Mori | |
| CN106605753A (en) | Method for extracting protein from asparagus | |
| CN106701883A (en) | Extraction method for bunge pricklyash leaf protein | |
| CN106892989A (en) | A kind of blackberry, blueberry preparation method | |
| CN104435062B (en) | A kind of blueberry wine mud extract for inducing apoptosis on hepatic stellate cells and its preparation technology and application | |
| CN105985873A (en) | Pine cone-cherokee rose fruit yellow rice wine and brewing method thereof | |
| CN105200107B (en) | The extracting method of Onchidium struma muscle crude protein | |
| CN112125948B (en) | Method for synchronously extracting plant polyphenol and plant protein from plant leaves and preparing plant protein hydrolysis peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170503 |