CN102071180A

CN102071180A - New process for preparing pineapple bromelain

Info

Publication number: CN102071180A
Application number: CN200910154652XA
Authority: CN
Inventors: 吴菁; 史利斌
Original assignee: HUZHOU LAISE BIOLOGICAL GENE ENGINEERING Co Ltd
Current assignee: HUZHOU LAISE BIOLOGICAL GENE ENGINEERING Co Ltd
Priority date: 2009-11-23
Filing date: 2009-11-23
Publication date: 2011-05-25

Abstract

The invention provides a new process for preparing pineapple bromelain, which comprises the following steps of: squeezing to obtain pineapple juice, cleaning pineapple leftovers, squeezing out juice by using a squeezer, collecting the juice, and adding 0.05 percent sodium benzoate for preservation; centrifuging the juice for 30 minutes in a centrifugal machine at a speed of 200r/min, and removing impurities; performing ultrafiltration concentration, namely introducing supernatant liquid into tubular ultrafiltration equipment, and performing ultrafiltration concentration under the pressure of 0.2MPa to make the volume concentrated to be 1/5 of the original volume; extracting a sediment by using tannin, namely immediately adding 0.1 percent tannin, standing at 4 DEG C for 1 hour, centrifuging to remove supernate, and collecting the sediment; and eluting the sediment by using solution of ascorbic acid, and performing vacuum freeze drying to obtain the pineapple bromelain product. The process is easy to operate, raw materials are readily available, product quality is good, the production cost is low, pollution does not exist in the production process, and the process is suitable for industrial production.

Description

A kind of bromeline new preparation process

Technical field

The invention provides a kind of bromeline new preparation process, relate to a kind of novel method of from the pineapple tankage, extracting bromeline.

Technical background

Bromeline (Bromelain is called for short Traumanase, also is called bromelin or pineapple ferment) is to extract from pineapple stem end, leaf, skin, through refining, purify, concentrate, enzyme immobilization, lyophilize and a kind of natural plant enzyme of obtaining.Its outward appearance is light grey Powdered, and molecular weight is 33000, and iso-electric point is 9.55.Marcano discovered in 1891 and contains proteolytic enzyme in the pineapple juice.Subsequently, people have launched a series of researchs to bromeline, find that it has good utility value at medicine and chemical field.Nineteen fifty-seven, Heineche etc. extract from pineapple stem and obtain proteolytic enzyme, thereby make bromeline realize commercialization production.At present, some functional components of bromeline separation of having succeeded, and be applied to field of medicaments.Along with the continuous progress of extracting purification technique, highly active bromeline will be widely used in medicine, chemical industry and field of food.

Domestic and international many scholars should be used as big quantity research to it at field of medicaments.The result shows, bromeline has important pharmaceutical use: bromeline has immunostimulation, the inside and outside studies show that, by to the hormesis of helper with to the direct inhibited reaction of T cell, bromeline can strengthen the immune response with suppressor T cell; Can prevent and treat cardiovascular disorder, the decomposition of its energy accelerating fibers proteinogen, allevating angina pectoris and apoplexy; Bromeline can be treated oedema and inflammation effectively in various tissues, and can treat diarrhoea; Bromeline is used for decrustation after burn, can optionally remove Exfoliating Scrub, and normal skin is had no adverse effects, and decrustation is thorough, and is significant to the treatment burn; The bromelain endonuclease capable suppresses the growth of tumour cell, reduces toxin in conjunction with activity, and can suppress the secretion effect of heat-labile toxin and Toxins,exo-, cholera; Bromeline can be promoted the absorption of medicine, can improve its curative effect with various microbiotic couplings, and can promote the absorption of nutritive substance.Bromeline also has some other effect at field of medicaments, awaits further demonstration.Bromeline is as a kind of foodstuff additive in foodstuffs industry, and energy decomposing protein, peptide, ester and acid amides etc. can be used for meat tenderization, protolysate, clarify beer, cheese production etc.Bromeline has very strong hydrolysis ability to containing the more protein of tyrosine, is used for the tenderization process of Niu Jiagong, the agreeable to the taste sense that can shorten the time, improve beef.

The application of bromeline has very long history, and along with the progress of extracting purification technique, it will have widely uses.China's pineapple aboundresources is produced per year more than pineapple 1,000,000 t, and annual all have a large amount of pineapple peel, stem to be wasted.Simultaneously, China increases year by year to the demand of bromeline, particularly highly active bromeline.At present, China's year needs more than the bromeline kiloton, and therefore domestic production quantity not sufficient 10%, how with the highly active bromeline of low-cost extraction, is to need the further problem of research.The present invention combines ultrafiltration and concentration and traditional tannin precipitator method, makes the auxiliary material consumption significantly reduce, and production cost reduces greatly, is a kind of good industrialized production.

Summary of the invention

1. bromeline new preparation process, its feature comprises following step:

(1) pineapple juice squeezing

The pineapple tankage are cleaned, extruded juice with squeezing machine, collecting juice, to add 0.05% Sodium Benzoate anticorrosion, then juice placed the centrifugal 30min of whizzer 200r/min, removes impurity.

(2) ultrafiltration and concentration

Clear liquor is fed the tubular type ultrafiltration apparatus, and ultrafiltration and concentration under the 0.2mpa pressure condition makes volume be concentrated into 1/5 of original volume.

(3) the tannin precipitation is extracted

Add 0.1% tannin at once, leave standstill 1h under 4 ℃, centrifugal abandoning supernatant is received throw out.

(4) wash-out drying

Throw out is put in people's dissolving vessel, added 2-3 times of pH value and be 4.5 ascorbic acid solution, stir wash-out 40min, filter then, collect filtrate, drying under reduced pressure or vacuum freezedrying promptly get the bromeline elaboration.

The innovative point of this method is that the concentrated solution behind the ultrafiltration and concentration extracts with the tannin method again, can save the consumption of other auxiliary materials so greatly, and total cost of production reduces, and good product quality, purity height, enzyme activity height, enzyme total yield height alive, economic benefit obviously is better than traditional technology.

Claims

1. bromeline new preparation process, its feature comprises following step:

(1) pineapple juice squeezing

(2) ultrafiltration and concentration

(3) the tannin precipitation is extracted

Add 0.1% tannin at once, under 4 ℃, leave standstill 1h, centrifugal abandoning supernatant, collecting precipitation thing.

(4) wash-out drying

2. tell as claim (1), it is characterized in that extracting raw material and adopt the pineapple tankage.

3. tell as claim (2), it is characterized in that ultra-filtration membrane selection polyacrylonitrile film (PAN), its molecular weight cut-off is 20000, and the bromeline rejection reaches 98%.

4. tell as claim (3), it is characterized in that the extraction of the combining ultrafiltration tannin precipitator method, supplementary product consumption is significantly reduced.

5. tell as claim (3), it is characterized in that the tannin consumption is 0.1%.

6. tell as claim (4), it is characterized in that pH value is that 4.5 anti-hematic acid solution can substitute with the hydrochloric acid soln of pH value 3.0.