CN101619095B - Preparation method of soybean polysaccharide protein in soybean dregs - Google Patents
Preparation method of soybean polysaccharide protein in soybean dregs Download PDFInfo
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- CN101619095B CN101619095B CN2009100417339A CN200910041733A CN101619095B CN 101619095 B CN101619095 B CN 101619095B CN 2009100417339 A CN2009100417339 A CN 2009100417339A CN 200910041733 A CN200910041733 A CN 200910041733A CN 101619095 B CN101619095 B CN 101619095B
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Abstract
The invention discloses a preparation method of soybean polysaccharide protein in soybean dregs. The preparation method comprises the following steps: taking soybean dregs produced after soybeans are processed as raw materials, extracting the soybean dregs in hot water, hydrolyzing cellulase enzyme, subsequently concentrating, drying and packaging to obtain the polysaccharide protein. In the preparation method, the extraction conditions are moderate, the extraction ratio is more than twice higher than that of a general method, and the obtained polysaccharide protein has complete molecule structure and high bioactivity; and meanwhile, the invention effectively utilizes the soybean dregs, greatly promotes the economic value of the soybean dregs and changes the soybean dregs into valuables.
Description
Technical field
The present invention relates to polysaccharide protein process for extracting in a kind of the soybean polysaccharide protein extracting method, particularly bean dregs.
Background technology
Soybean is edible both, can extract oil again, is called as " flower of nutrition ", " kings in the beans " and " Tanaka's meat ", is the food that praised highly by the nutritionist in hundreds of natural foods.
In the present soybean process for processing, mainly utilize its fat and protein portion., soybean oil and Sunlover 10 can produce a large amount of bean dregs after machining, as producing 30%～35% bean dregs in the processing of soybean protein isolate.The main composition of bean dregs is cell wallss of cotyledon portion, contains the soybean polysaccharide albumen of 30% (being as the criterion with bean dregs) in the cell walls approximately.Soybean polysaccharide albumen has very high nutritive value, has good health-care effect.The bean dregs majority is taken as feed, fertilizer or offal treatment now, does not well carry out deep processing, not only wastes resource, and possibly cause the social environment problem.
Soybean polysaccharide albumen is present in the cell walls of soybean, and is tight with cell wall-bound, and the General Physics processing and treating method is difficult to obtain.In existing soybean polysaccharide albumen sepn, extraction and the purge process, mainly adopt the hot water extraction, promptly handle, obtain the proteic raw product of soybean polysaccharide through processes such as boiling water extraction, ethanol sedimentation, dialysis and column chromatographies.This technical process is numerous and diverse, need use a large amount of ethanol and acid & alkali liquid in the production process, and extraction yield is being merely 15～20%, and production cost is high, and the waste water that produces can pollute environment.Because more a large amount of use acid-base solutions, reaction conditions is violent, the soybean polysaccharide albumen that this method is produced, and molecular structure has destruction to a certain degree, and protein-active reduces, and influences the proteic effect of soybean polysaccharide.
Summary of the invention
In order to address the above problem, the present invention provides that a kind of production cost is low, pollution-free, high activity soy bean polysaccharide protein process for extracting.
Technical scheme of the present invention is:
The preparation method of soybean polysaccharide protein in soybean dregs may further comprise the steps:
1) preparation virgin pulp liquid: get bean dregs and add water, be heated to 80～150 ℃, insulation lixiviate 1～3h is cooled to suitable enzymolysis then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, add cellulase, enzymolysis cell walls free polysaccharide albumen with enzyme-deactivating, obtains enzymolysis solution after enzymolysis is accomplished;
3) subsequent disposal: with enzymolysis solution concentrate, dry, be packaged to be product.
The invention has the beneficial effects as follows: adopt the hot water high-temp extracting, softening cell walls adopts enzymolysis processing then, and extraction yield is high, and reaction conditions is gentle, and the polysaccharide protein structural integrity that obtains is active high; Need not add a large amount of ethanol and acid & alkali liquid in the leaching process, reduce production cost, avoid possible environmental pollution.Simultaneously, effectively utilized bean dregs, promoted the economic worth of bean dregs greatly, realized " becoming slag " into precious.
Embodiment
Below in conjunction with instance, further specify the present invention.
The model of the cellulase of using in the following instance is Xm66d, Xm90, produces by Ningxia jade of the He family Bioisystech Co., Ltd, or the plain enzyme of the ENERGEX L fiber type of Denmark Novo Nordisk company production; Bean dregs are provided by the holy bio tech ltd in sea, Guangzhou.
Embodiment 1
1) preparation virgin pulp liquid: get soybean bean dregs 1000 grams after the oven dry, adds 8000 milliliters water, be heated to 80 ℃, the back that stirs is incubated lixiviate 1h, is cooled to 40 ℃ then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm66d cellulase of bean dregs dry weight 0.35%, and under 40 ℃, enzymolysis 1h free polysaccharide albumen is warming up to 90～100 ℃ then rapidly, and 1min is with the cellulase deactivation in insulation, obtains enzymolysis solution;
3) filter:, reduce macromole foreign matter content in the enzymolysis solution the diatomite filtration of enzymolysis solution through one deck 0.3cm;
4) desalination: the enzymolysis solution after will filtering is through reverse osmosis machine, crosses after the tubular membrane after one time 5000 molecular film, and small-molecule substances such as salt and monose, amino acid are removed, and collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 71%, and extraction yield is 37%.
Embodiment 2
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 14000 milliliters water, be heated to 90 ℃, the back that stirs is incubated lixiviate 1.5h, is cooled to 40 ℃ then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm66d cellulase of bean dregs dry weight 0.45%, and under 40 ℃, enzymolysis 1h free polysaccharide albumen is warming up to 90～100 ℃ rapidly, and 1min is with the cellulase deactivation in insulation, obtains enzymolysis solution;
3) filter:, reduce macromole foreign matter content in the enzymolysis solution the diatomite filtration of enzymolysis solution through one deck 0.4cm;
4) desalination: the enzymolysis solution after will filtering after one time 3000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt hot air seasoning dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 78%, and extraction yield is 39%.
Embodiment 3
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 18000 milliliters water, be heated to 100 ℃, the back that stirs is incubated lixiviate 1h, is cooled to 50 ℃ then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm90 cellulase of bean dregs dry weight 0.35%, and under 50 ℃, enzymolysis 1.5h is warming up to 90～100 ℃ rapidly, and 1min is with the cellulase deactivation in insulation, obtains enzymolysis solution;
3) filter:, reduce macromole foreign matter content in the enzymolysis solution the diatomite filtration of enzymolysis solution through one deck 0.3cm;
4) desalination: the enzymolysis solution after will filtering after one time 3000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 76%, and extraction yield is 38%.
Embodiment 4
1) preparation virgin pulp liquid: get soybean bean dregs 3000 grams after the oven dry, adds 24000 milliliters water, be heated to 110 ℃, the back that stirs is incubated lixiviate 1.5h, is cooled to 40 ℃ then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm66d cellulase of bean dregs dry weight 0.45%, and under 40 ℃, enzymolysis 1.5h is warming up to 90～100 ℃ rapidly, and 1min is with enzyme-deactivating in insulation, obtains enzymolysis solution;
3) filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.5cm;
4) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt vacuum freeze-drying method dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 83%, and extraction yield is 41%.
Embodiment 5
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 20000 milliliters water, be heated to 120 ℃, the back that stirs is incubated lixiviate 3h, is cooled to 50 ℃ then, obtains virgin pulp liquid.
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm90 cellulase of bean dregs dry weight 0.25%, and under 50 ℃, enzymolysis 2h is warming up to 90～100 ℃ rapidly, and 1min is with the cellulase deactivation in insulation, obtains enzymolysis solution.
3) filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.4cm;
4) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 73%, and extraction yield is 38%.
Embodiment 6
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 12000 milliliters water, be heated to 130 ℃, the back that stirs is incubated lixiviate 2h, is cooled to 50 ℃ then, obtains virgin pulp liquid.
2) enzymolysis: in virgin pulp liquid, adding quality is the Xm90 cellulase of bean dregs dry weight 0.25%, and under 50 ℃, enzymolysis 2h is warming up to 90～100 ℃ rapidly, and 1min is with the cellulase deactivation in insulation, obtains enzymolysis solution.
3) filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.4cm;
4) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 77%, and extraction yield is 39%.
Embodiment 7
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 18000 milliliters water, be heated to 150 ℃, the back that stirs is incubated lixiviate 2h, is cooled to 45 ℃ then, obtains virgin pulp liquid.
2) enzymolysis: in virgin pulp liquid, adding quality is the plain enzyme of ENERGEX L fiber type of bean dregs dry weight 0.25%, and under 45 ℃, enzymolysis 2h is warming up to 90～100 ℃ rapidly, and 1.5min is with the cellulase deactivation in insulation, obtains enzymolysis solution.Filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.4cm;
3) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
4) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
5) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
6) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 79%, and extraction yield is 39%.
Embodiment 8
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 17000 milliliters water, be heated to 135 ℃, the back that stirs is incubated lixiviate 2.5h, is cooled to 45 ℃ then, obtains virgin pulp liquid.
2) enzymolysis: in virgin pulp liquid, adding quality is the plain enzyme of ENERGEX L fiber type of bean dregs dry weight 0.15%, and under 45 ℃, enzymolysis 2h is warming up to 90～100 ℃ rapidly, and 1.5min is with the cellulase deactivation in insulation, obtains enzymolysis solution.
3) filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.4cm;
4) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 77%, and extraction yield is 39%.
Embodiment 9
1) preparation virgin pulp liquid: get soybean bean dregs 2000 grams after the oven dry, adds 16000 milliliters water, be heated to 140 ℃, the back that stirs is incubated lixiviate 2.5h, is cooled to 45 ℃ then, obtains virgin pulp liquid.
2) enzymolysis: in virgin pulp liquid, adding quality is the plain enzyme of ENERGEX L fiber type of bean dregs dry weight 0.30%, and under 45 ℃, enzymolysis 2h is warming up to 90～100 ℃ rapidly, and 1.5min is with the cellulase deactivation in insulation, obtains enzymolysis solution.
3) filter:, reduce its macromole foreign matter content the diatomite filtration of enzymolysis solution through one deck 0.4cm;
4) desalination: the enzymolysis solution after will filtering after one time 5000 molecular film, is removed salt and small-molecule substance after the mistake tubular membrane through reverse osmosis machine, collects the soybean polysaccharide protein liquid that obtains desalination;
5) decolouring: with the processing of decolouring of the ion exchange resin column of the soybean polysaccharide protein liquid after the desalination through the MN200 type;
6) deodorizing: the enzymolysis solution of handling that will decolour passes through activated carbon column, removes the peculiar smell in the enzymolysis solution;
7) subsequent disposal: the soybean polysaccharide protein liquid after the deodorizing is concentrated through the falling liquid film thickner, the soybean polysaccharide protein liquid is concentrated into about 25%, adopt spray-drying process dry then, obtain Powdered soybean polysaccharide albumen, be packaged to be product.
Through measuring and calculating, the proteic content of the soybean polysaccharide of product is 82%, and extraction yield is 41%.
More than the product of each embodiment: the soybean polysaccharide protein powder, look white, free from beany flavor, polysaccharide protein content is more than 70%.Owing to adopt the cellulase hydrolysis cell walls, make polysaccharide protein dissociate out, reaction conditions is gentle, and the polysaccharide protein molecular structure that obtains is complete, has kept the biological activity of polysaccharide protein; Simultaneously, also improved the extraction yield of polysaccharide protein greatly, the proteic extraction yield of soybean polysaccharide of the present invention is 38～40%, is more than 2 times of usual way, effectively raises production efficiency.
Claims (10)
1. the preparation method of soybean polysaccharide protein in soybean dregs may further comprise the steps:
1) preparation virgin pulp liquid: get bean dregs and add water, be heated to 80～150 ℃, insulation lixiviate 1～3h is cooled to suitable enzymolysis then, obtains virgin pulp liquid;
2) enzymolysis: in virgin pulp liquid, add cellulase, enzymolysis cell walls free polysaccharide albumen with the cellulase deactivation, obtains enzymolysis solution after enzymolysis is accomplished;
3) subsequent disposal: with enzymolysis solution concentrate, dry, be packaged to be product.
2. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 1 is characterized in that: obtain after the enzymolysis solution through enzymolysis processing, decolour after the processing, carry out subsequent disposal again, obtain product.
3. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 2 is characterized in that: enzymolysis solution decolour handle before, also to carry out desalting treatment, to improve decolorizing efficiency.
4. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 3 is characterized in that: select reverse osmosis method during desalting treatment for use.
5. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 3 is characterized in that: virgin pulp liquid filters earlier to reduce the macromole foreign matter content in the virgin pulp liquid before carrying out desalting treatment.
6. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 2 is characterized in that: the decolouring enzymolysis liquor was carried out deodorizing and is handled before subsequent disposal.
7. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 1 is characterized in that: select the falling liquid film method of enrichment when enzymolysis solution concentrates for use.
8. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 1 is characterized in that: select spray-drying process, hot air seasoning or vacuum freeze-drying method when dry for use.
9. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 1 is characterized in that: during the preparation virgin pulp liquid, the weight ratio of the water of bean dregs dry weight and adding is 1:6～10.
10. the preparation method of soybean polysaccharide protein in soybean dregs according to claim 1, it is characterized in that: the time of enzymolysis is 1～2h.
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| CN106173741A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | Soybean dietary fiber beverage and preparation technology thereof |
| CN106509330A (en) * | 2016-09-24 | 2017-03-22 | 合肥信达膜科技有限公司 | Extraction technology of monellin |
| CN106566859A (en) * | 2016-11-02 | 2017-04-19 | 大连豪翔生物酶工程有限公司 | Method of producing soybean glycopeptides with soybean dregs as raw material |
| CN107183306A (en) * | 2017-07-14 | 2017-09-22 | 山东禹王生态食业有限公司 | A kind of soybean protein powder producing method with intestinal regulatory function |
| CN108929892B (en) * | 2018-08-02 | 2021-12-10 | 扬州市龙伟食品有限公司 | Method for extracting soluble bean dregs protein and polypeptide from bean dregs |
| CN110343156B (en) * | 2019-07-26 | 2021-02-26 | 山西农业大学 | Extraction and purification method of black bean glycoprotein |
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| 司方.酶法提取豆渣水溶性膳食纤维的研究.《农产品加工 学刊》.2009, * |
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Denomination of invention: Preparation method of soybean polysaccharide protein in soybean residue Effective date of registration: 20230919 Granted publication date: 20120328 Pledgee: Bank of China Limited Guangzhou Pearl River Branch Pledgor: GUANGZHOU FOBIBER BIOLOGICAL INDUSTRY Co.,Ltd. Registration number: Y2023980057418 |