CN104286856A - Production method of odorless high-purity soybean oligopeptide - Google Patents
Production method of odorless high-purity soybean oligopeptide Download PDFInfo
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- CN104286856A CN104286856A CN201410562330.XA CN201410562330A CN104286856A CN 104286856 A CN104286856 A CN 104286856A CN 201410562330 A CN201410562330 A CN 201410562330A CN 104286856 A CN104286856 A CN 104286856A
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- peptide
- enzymolysis
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- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 89
- 244000068988 Glycine max Species 0.000 title claims abstract description 87
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 44
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 44
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 230000009965 odorless effect Effects 0.000 title abstract 2
- 239000007788 liquid Substances 0.000 claims abstract description 70
- 235000018102 proteins Nutrition 0.000 claims abstract description 64
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 64
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 64
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 63
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 59
- 238000000034 method Methods 0.000 claims abstract description 44
- 239000011347 resin Substances 0.000 claims abstract description 38
- 229920005989 resin Polymers 0.000 claims abstract description 38
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 35
- 238000000909 electrodialysis Methods 0.000 claims abstract description 32
- 239000007787 solid Substances 0.000 claims abstract description 31
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 27
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 17
- 238000001694 spray drying Methods 0.000 claims abstract description 12
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 7
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 7
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 6
- 230000000415 inactivating effect Effects 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 56
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- 238000002156 mixing Methods 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 35
- 239000000463 material Substances 0.000 claims description 34
- 239000002253 acid Substances 0.000 claims description 25
- 239000000706 filtrate Substances 0.000 claims description 25
- 239000000047 product Substances 0.000 claims description 22
- 108091005804 Peptidases Proteins 0.000 claims description 21
- 239000004365 Protease Substances 0.000 claims description 21
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 21
- 239000000796 flavoring agent Substances 0.000 claims description 21
- 235000019634 flavors Nutrition 0.000 claims description 21
- 239000000843 powder Substances 0.000 claims description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
- 239000012141 concentrate Substances 0.000 claims description 20
- 238000010612 desalination reaction Methods 0.000 claims description 20
- 239000007791 liquid phase Substances 0.000 claims description 17
- 238000001179 sorption measurement Methods 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- 239000002075 main ingredient Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 14
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 14
- 235000013305 food Nutrition 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 11
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 230000009849 deactivation Effects 0.000 claims description 10
- 239000003337 fertilizer Substances 0.000 claims description 10
- 239000012065 filter cake Substances 0.000 claims description 10
- 238000005342 ion exchange Methods 0.000 claims description 10
- 229940001941 soy protein Drugs 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 235000020995 raw meat Nutrition 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 7
- 239000006227 byproduct Substances 0.000 claims description 6
- 241000234435 Lilium Species 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 125000003158 alcohol group Chemical group 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 235000014633 carbohydrates Nutrition 0.000 claims description 5
- 150000001720 carbohydrates Chemical class 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 5
- 235000010980 cellulose Nutrition 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- 238000006911 enzymatic reaction Methods 0.000 claims description 5
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 238000001764 infiltration Methods 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 229910021645 metal ion Inorganic materials 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- 230000035484 reaction time Effects 0.000 claims description 5
- 238000004064 recycling Methods 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 238000000638 solvent extraction Methods 0.000 claims description 5
- 238000003860 storage Methods 0.000 claims description 5
- 230000000717 retained effect Effects 0.000 claims description 2
- 235000019764 Soybean Meal Nutrition 0.000 abstract description 2
- 239000004455 soybean meal Substances 0.000 abstract description 2
- 108010007119 flavourzyme Proteins 0.000 abstract 2
- 238000000746 purification Methods 0.000 abstract 2
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000001877 deodorizing effect Effects 0.000 abstract 1
- 238000011033 desalting Methods 0.000 abstract 1
- 235000013402 health food Nutrition 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 229910017053 inorganic salt Inorganic materials 0.000 abstract 1
- 230000006870 function Effects 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 240000003211 Corylus maxima Species 0.000 description 1
- 229930187719 Soyasaponin Natural products 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a production method of odorless high-purity soybean oligopeptide, belonging to the field of health food, and aiming at overcoming shortcomings that an existing soybean peptide product is odorous, poor in color and low in peptide purity. An extracting method taking soybean meal as a raw material comprises the following steps of removing odor, decolorizing, adding 'flavourzyme' for enzymatic hydrolysis, separating out protein which is not enzymatically hydrolyzed through acid precipitation, separating solid and liquid ingredients after enzymatic hydrolysis, removing inorganic salt through electrodialysis, performing resin purification and reverse osmosis concentration, decolorizing and deodorizing through activated carbon, and spray-drying to obtain the finished product. An extracting method taking soy isolate protein as a raw material comprises the following steps of dissolving protein, adding flavourzyme, hydrolyzing soybean protein, sterilizing and inactivating enzyme, filtering, purifying, desalting through electrodialysis, performing resin purification and reverse osmosis concentration, decolorizing through activated carbon, and spray-drying to obtain the finished product. The production method has the beneficial effects that the prepared soybean oligopeptide is high in purity, and free from odor and foreign color, thus improving product grade, widening market of the soybean oligopeptide products and offering a high-quality health product for the public.
Description
Technical field
The invention belongs to field of health care food, be specifically related to deep processing soybean and extract health-care components.
Background technology
At present, commercially available Soybean Peptide type of merchandize is various, of many uses, but, be no matter be raw material with dregs of beans, or be that the Soybean Peptide that raw material is produced all exists three large defects with protein isolate:
(1) peculiar smell of enzymolysis protein generation, affects edible quality
After soybean protein enzymolysis, special raw meat, hardship, the stink of normal generation, Soybean Peptide is in production process, because inorganic salts remove not thorough, the salts such as residual NaCl make Soybean Peptide finished product also remain with certain saline taste, feed in raw material as " food " or food additive, raw meat, hardship, the peculiar smell such as smelly, salty, have a strong impact on the edible quality of Soybean Peptide itself and be added the product quality of food, not possessing foodstuffs for human and eat, cause the joyful feature of the human body sense of taste;
(2) " peptide " purity is low, hinders the performance of " peptide " effect
China's soy peptide powder " national standard " (GB/T22492-2008) specifies: " peptide content (in butt) is 55% ~ 80% ", namely the amino acid residue compounds content of relative molecular mass≤5000 reaches more than 55%, namely can be described as " peptide ", visible China " Soybean Peptide " series products, because purity is not high, when edible dosage is identical, the performance of effect of Soybean Peptide must be affected;
(3) color and luster is not good enough, and marketing quality is declined
" Soybean Peptide " sterling, color and luster should be pure white, but current commercial goods, thorough owing to being separated other material in production process, residual colour generation thing often makes commodity soybean peptide be filbert or light yellow, because color and luster is not good enough, and commodity image is declined.
Summary of the invention
The object of this invention is to provide a kind of with the production method of Soybean Meal or the soybean protein isolate free from extraneous odour that is raw material, highly purified soybean oligopeptide.Overcome the above-mentioned shortcoming that existing Soybean Peptide product exists.
In the first invention be that the method that raw material extracts " free from extraneous odour, highly purified soybean oligopeptide " comprises with dregs of beans:
(1) remove in peculiar smell and present-color material:
Fat is remained≤2.5%, protein content >=50%, NSI value >=80% bean cake powder be broken into 40-60 object meal, send in the pot for solvent extraction of belt stirrer, add again concentration 55% ~ 60% ethanol water, addition is that dregs of rice material weighs 7 ~ 10 times, temperature is kept to be 50 DEG C ~ 60 DEG C, mixing speed 30 ~ 60r/min, mixing time 2 ~ 8h;
(2) soybean protein and colour generation, extract respectively in peculiar smell thing:
To complete (1) step adds in the mixed liquor feeding centrifugal separator of ethanol dissolving, under rotating speed about 3500-4500r/min condition, carry out centrifugation, the liquid phase thing flowed out is the solution containing the soybean complex function factor, being separated and retaining Main Ingredients and Appearance is alcohol insoluble protein (in butt protein content >=65%) and a small amount of solid formation for polymeric carbohydrates such as non-prolamine celluloses, has abandoned peculiar smell, coloured liquid phase thing or has retained deep processing and extracted byproduct;
(3) recycling design ethanol:
(2) step is separating obtained based in the humidity material of alcohol insoluble protein feeding vacuum desiccator through seperator, the vacuum of vacuum desiccator is 77.3kpa, temperature is 70-90 DEG C, processing time 1 ~ 1.5h, be recycled in ethanol storage tank through vacuum desiccator gained ethanol evaporation, continue to be cycled to used in production;
(4) " flavor protease " enzymolysis:
After being reclaimed by (3) step gained ethanol distillation, dry substance concentration is the mixed liquor of ≈ 20%, send in the enzymatic vessel of belt stirrer, be diluted with water, dilution dry substance concentration is 3-7%, stir while add waterside, mixing speed 30 ~ 60r/min, after stirring, add " flavor protease ", time enzyme-added, mixing speed reduces to 25-35r/min, and enzyme concentration is 1% ~ 5% of total solid, water temperature keeps 50-60 DEG C, pH keeps 7.2, and the enzyme reaction time is 3 ~ 6h, and in this step enzymolysis gained mixed liquor, Main Ingredients and Appearance is the peptide solution in solution state;
(5) albumen and other water-insoluble solid content of non-enzymolysis is removed:
(4) step gained do not had raw meat, smelly, the enzymolysis protein mixed liquor rapid temperature increases of waiting bitterly for peculiar smell to >=80 DEG C, heats 20-40 minute, carry out enzyme deactivation process;
(6) " acid is heavy " separates out non-enzymolysis protein:
Pump in sour deep gouge by (5) step gained enzyme deactivation mixed liquor, add food grade hydrochloric acid, acid adding limit, limit is stirred, mixing speed is 30 ~ 60r/min, mixed liquor pH value is adjusted to 4.2 ~ 4.5, under soy protein isoelectric point condition, makes a small amount of non-enzymolysis protein be that curdy is separated out;
(7) the solid, liquid composition after enzymolysis is separated:
(6) step is sunk the mixed liquor of process through acid and pumps into centrifugal separator or the Soybean Peptide aqueous solution based on peptide is separated with non-water-soluble solid content by isolated by filtration machine at whipping process, when adopting plate and frame filter press, pressure is 0.1 ~ 1.0MPa, flow velocity 800-1200L/h, filter aid is diatomite, filtered solution should reach transparent degree, reserved filtrate, abandons filter cake or transfers to fertilizer, feed;
(8) inorganic salts are removed in electrodialysis
It is 2% that the transparent clear liquid that (7) step obtains is added water move to concentration, pump into ion-exchange unit, the acid ion in solution and metal ion is made to be adsorbed in ion-exchange unit respectively, when using electrodialysis plant feed pump pressure be 0.15-0.25Mpa, electric current 18A ~ 20A, after solution passes through, the electrical conductivity of material should≤300 μ s/cm
2;
(9) resin is purified and reverse osmosis concentration
The electrical conductivity obtained through step (8) electrodialysis desalination is reached 100 ~ 300 μ s/cm
2feed liquid send in nonpolar CAD40 resin column, be highly purified soybean oligopeptide solution by the liquid phase thing of CAD40 resin column, pump into φ≤10
-10m counter-infiltration (RO) device, efflux is pure water, capable of circulation for the production of, trapped fluid is the highly purified soybean oligopeptide concentrate of concentration ≈ 10%;
(10) activated carbon decolorizing, de-taste
(9) step gained soybean oligopeptide concentrate is added activated carbon decolorizing, and activated carbon grain addition is about the 1-3% of concentrate, the concentrate after active carbon adsorption decolouring, then filters through plate and frame filter press, can obtain colourless underflow liquid;
(11) spraying dry
Colourless, free from extraneous odour, the clear solution that (10) step are obtained, through φ≤10
-7send into after micropore (MF) filters in press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, products obtained therefrom after the spray-dried tower process of feed liquid, is " free from extraneous odour, the highly purified soybean oligopeptide powder " of peptide component content>=80% of free from extraneous odour, peptide purity>=90%, relative molecular mass≤1000.
The second invention is that the method for raw material extraction " free from extraneous odour, highly purified soybean oligopeptide " comprising with soybean protein isolate:
(1) soluble protein
Dropped into by soybean protein isolate in the dissolving tank of belt stirrer, input limit, limit is stirred, and mixing speed is 30 ~ 60r/min, and protein isolate and water ratio are 5:90 ~ 100, and water temperature remains between 50 ~ 60 DEG C, stirs 30 ~ 120min;
(2) flavor protease is added, hydrolytic soya bean protein
Add flavor protease in step (1) process, mixing material in dissolved state, enzyme concentration is 1% ~ 5% of protein isolate weight, and coolant-temperature gage remains between 50 ~ 60 DEG C, continues stirring 4 ~ 6h;
(3) sterilising and enzyme inactivating
To complete the protein liquid of enzymolysis through (2) step, heat, heating-up temperature is 80 ~ 90 DEG C, stirs simultaneously, and mixing time is about 30min;
(4) filter, purify
Enzymolysis protein liquid through step (3) is cooled to less than 40 DEG C, adds food grade hydrochloric acid and be adjusted to soy protein isoelectric point pH4.2 ~ 4.5, stir the soybean protein precipitating making non-enzymolysis under 40r ~ 60r/min condition; Again by through waiting mixed liquor of the heavy process of electricity acid to send into flame filter press or filter centrifugal, when using flame filter press, pressure is 0.1 ~ 1.0MPa, flow velocity is 1000L/h, filter aid is diatomite, mixed liquor is separated by filtration or the liquid phase thing (enzymatic hydrolysis of soybean peptide liquid) of centrifugation gained all to reach transparent level for degree, reserved filtrate, abandons the solid formation that retains or transfers to feed or fertilizer;
(5) electrodialysis desalination
The colourless transparent liquid (4) step obtained sends into electrodialysis plant, and feed pump pressure is 0.15-0.25MPa, and electrodialysis plant electric current is 18A ~ 20A, and after desalination, the electrical conductivity of material answers 100 ~ 300 μ s/cm
2;
(6) resin is purified
Electrical conductivity after (5) step desalination is answered 100 ~ 300 μ s/cm
2, light brown yellow liquid pumps in non-polar resin column device, abandon resin adsorption thing or reserve for other use as byproduct.
(7) reverse osmosis concentration
The liquid that (6) step flows out after resin adsorption is pumped into φ≤10
-10reverse osmosis unit (RO), by reverse-osmosis treated, efflux is pure water, can use at workshop Inner eycle, after reverse-osmosis treated, take Soybean Peptide as the solution concentration 8-12% of Main Ingredients and Appearance;
(8) activated carbon decolorizing
(7) step gained liquid is pumped into the bleacher of belt stirrer, add active carbon by 2% of amount of solution, stir 20 ~ 30min, then the solution being mixed with active carbon is sent into plate and frame filter press, at pressure≤3.5Kg/cm
2condition circulating filtration, be colourless to filtrate, reserved filtrate, abandon filter cake or as byproduct he use;
(9) spraying dry is finished product
Be the slurries of Soybean Peptide by the solute of (8) step gained, through φ≤10
-7after micropore (MF) filters, gained filtrate sends into press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, the lily powder of the dry gained of the spray-dried tower of feed liquid, for the composition of relative molecular mass≤1000 distributes>=80%, composition total content>=90% of peptide, " free from extraneous odour, the highly purified soybean oligopeptide powder " of free from extraneous odour.
Good effect of the present invention is: key technique of the present invention be by adding ethanolic solution in material protein, can be dissolved in ethanol, in peculiar smell and other alcohol dissolubility present-color material, as isoflavones, soyasaponin (saponin(e sterling be white but in compound situation, normal in bronzing) etc. organic component be separated completely with the soybean protein of ethanol with insoluble or slightly soluble, obtaining the high purity protein raw material of free from extraneous odour, is prerequisite of the present invention.Use, commodity " flavor protease " enzymolysis protein, gained enzymolysis protein product do not have raw meat, smelly, wait bitterly for peculiar smell, what ensure that on final products smell is pure.Again by resin adsorption and press filtration or centrifugation, make a living production of high purity soybean oligopeptide and product NSI value=100%, solubility=100% create prerequisite material condition;
By above key technology means, obtain the soybean oligopeptide of high-purity, free from extraneous odour, non-variegation, improve product hierarchy, widened soybean oligopeptide produce market, for the public provides high-quality health products.
Be raw material with soybean protein isolate in the present invention, for small investment, not with comprehensive utilization be target, simple take Soybean Peptide as the manufacturing enterprise of major product, adopt the method more applicable, because production technology link reduces, wastewater discharge is few, production cycle shortening, and makes production cost reduce.
The product of the present invention of Food Inspection authority checks identification of authorizing through country physical and chemical index of being correlated with contrasts as follows with national standard (GB/T 22492-2008) list relating to this kind of product
Detailed description of the invention
Embodiment 1 is that raw material extracts one of the method for " free from extraneous odour, highly purified soybean oligopeptide " with dregs of beans:
(1) remove in dregs of beans in peculiar smell and present-color material:
Fat is remained≤2.5%, protein content >=50%, NSI value >=80% bean cake powder be broken into 50 object meal, send in the pot for solvent extraction of belt stirrer, add the ethanol water of concentration at 55-60% again, addition is that dregs of rice material weighs 9 times, temperature is kept to be 55 DEG C, mixing speed 45r/min, mixing time 5h;
(2) soybean protein and colour generation, extract respectively in peculiar smell thing:
To complete (1) step adds in the mixed liquor feeding centrifugal separator of ethanol dissolving, under rotating speed about 4000r/min condition, carry out centrifugation, the liquid phase thing flowed out is the solution containing the soybean complex function factor, and being separated and retaining Main Ingredients and Appearance is alcohol insoluble protein (in butt protein content >=65%) and a small amount of solid formation for polymeric carbohydrates such as non-prolamine celluloses;
(3) recycling design ethanol:
(2) step is separating obtained based in the humidity material of alcohol insoluble protein feeding vacuum desiccator through seperator, the vacuum of vacuum desiccator is 77.3kpa, temperature is 80 DEG C, processing time 1 ~ 1.5h, feed liquid is concentrated into concentration about 20%, be recycled in ethanol storage tank through vacuum desiccator gained ethanol evaporation, continue to be cycled to used in production.
(4) " flavor protease " enzymolysis:
By (3) step gained mixed liquor, send in the enzymatic vessel of belt stirrer, be diluted with water, dilution dry substance concentration is about 5%, stirs, mixing speed 45r/min while add waterside, after stirring, add " flavor protease ", time enzyme-added, mixing speed reduces to 30r/min, and enzyme concentration is 3% of total solid, water temperature keeps 55 DEG C, pH keeps 7.2, and the enzyme reaction time is 4.5h, and in this step enzymolysis gained mixed liquor, Main Ingredients and Appearance is the peptide solution in solution state;
(5) albumen and other water-insoluble solid content of non-enzymolysis is removed:
(4) step gained do not had raw meat, smelly, the enzymolysis protein mixed liquor rapid temperature increases of waiting bitterly for peculiar smell to >=80 DEG C, heats 0.5h, carry out enzyme deactivation process;
(6) " acid is heavy " separates out non-enzymolysis protein:
Pump in sour deep gouge by (5) step gained enzyme deactivation mixed liquor, add food grade hydrochloric acid, acid adding limit, limit is stirred, mixing speed is 45r/min, mixed liquor pH value is adjusted to 4.2 ~ 4.5, under soy protein isoelectric point condition, makes a small amount of non-enzymolysis protein be that curdy is separated out;
(7) the solid, liquid composition after enzymolysis is separated:
(6) step is sunk the mixed liquor of process through acid and pumps into centrifugal separator or the Soybean Peptide aqueous solution based on peptide is separated with non-water-soluble solid content by isolated by filtration machine at whipping process, when adopting plate and frame filter press, pressure is 0.5MPa, flow velocity 1000L/h, filter aid is diatomite, filtered solution should reach transparent degree, reserved filtrate, abandons filter cake or give over to other to utilize;
(8) inorganic salts are removed in electrodialysis
It is 2% that the transparent clear liquid that (7) step obtains is added water move to concentration, pump into ion-exchange unit, the acid ion in solution and metal ion is made to be adsorbed in ion-exchange unit respectively, when using electrodialysis plant feed pump pressure be 0.2Mpa, electric current 18A ~ 20A, after solution passes through, the electrical conductivity of material should≤300 μ s/cm
2;
(9) resin is purified and reverse osmosis concentration
The electrical conductivity obtained through step (8) electrodialysis desalination is reached 200 μ s/cm
2feed liquid send in nonpolar CAD40 resin column, be highly purified soybean oligopeptide solution by the liquid phase thing of CAD40 resin column, pump into φ≤10
-10m counter-infiltration (RO) device, efflux is pure water, capable of circulation for the production of, trapped fluid is the highly purified soybean oligopeptide concentrate of concentration about 10%;
(10) activated carbon decolorizing, de-taste
(9) step gained soybean oligopeptide concentrate is added activated carbon decolorizing, and activated carbon grain addition is about 2% of concentrate, the concentrate after active carbon adsorption decolouring, then filters through plate and frame filter press, can obtain colourless underflow liquid;
(11) spraying dry
Colourless, free from extraneous odour, the clear solution that (10) step are obtained, through φ≤10
-7send into after micropore (MF) filters in press spray drying tower, the inlet temperature of tower is 180 DEG C, outlet temperature is 70 DEG C, products obtained therefrom after the spray-dried tower process of feed liquid, is " free from extraneous odour, the highly purified soybean oligopeptide powder " of peptide component content>=80% of free from extraneous odour, peptide purity>=90%, relative molecular mass≤1000.
Embodiment 2 take dregs of beans as the method two that raw material extracts " free from extraneous odour, highly purified soybean oligopeptide ":
(1) remove in dregs of beans in peculiar smell and present-color material:
Fat is remained≤2.5%, protein content >=50%, NSI value >=80% bean cake powder be broken into 40 object meal, send in the pot for solvent extraction of belt stirrer, add again concentration 55% ~ 60% ethanol water, addition is that dregs of rice material weighs 10 times, temperature is kept to be 60 DEG C, mixing speed 60r/min, mixing time 2h;
(2) soybean protein and colour generation, extract respectively in peculiar smell thing:
To complete (1) step adds in the mixed liquor feeding centrifugal separator of ethanol dissolving, under rotating speed about 4000r/min condition, carry out centrifugation, the liquid phase thing flowed out is the solution containing the soybean complex function factor, and being separated and retaining Main Ingredients and Appearance is alcohol insoluble protein (in butt protein content >=65%) and a small amount of solid formation for polymeric carbohydrates such as non-prolamine celluloses;
(3) recycling design ethanol:
(2) step is separating obtained based in the humidity material of alcohol insoluble protein feeding vacuum desiccator through seperator, the vacuum of vacuum desiccator is 77.3kpa, temperature is 90 DEG C, processing time 1 ~ 1.5h, feed liquid is concentrated into concentration about 20%, be recycled in ethanol storage tank through vacuum desiccator gained ethanol evaporation again, continue to be cycled to used in production.
(4) " flavor protease " enzymolysis:
By the mixed liquor that (3) step gained dry substance concentration is ≈ 20%, send in the enzymatic vessel of belt stirrer, be diluted with water, dilution dry substance concentration is about 5%, stir while add waterside, mixing speed 60r/min, after stirring, add " flavor protease ", time enzyme-added, mixing speed reduces to 30r/min, and enzyme concentration is 5% of total solid, water temperature keeps 55 DEG C, pH keeps 7.2, and the enzyme reaction time is 6h, and in this step enzymolysis gained mixed liquor, Main Ingredients and Appearance is the peptide solution in solution state;
(5) albumen and other water-insoluble solid content of non-enzymolysis is removed:
(4) step gained do not had raw meat, smelly, the enzymolysis protein mixed liquor rapid temperature increases of waiting bitterly for peculiar smell to >=80 DEG C, heats 20 minutes, carry out enzyme deactivation process;
(6) " acid is heavy " separates out non-enzymolysis protein:
Pump in sour deep gouge by (5) step gained enzyme deactivation mixed liquor, add food grade hydrochloric acid, acid adding limit, limit is stirred, mixing speed is 60r/min, mixed liquor pH value is adjusted to 4.2 ~ 4.5, under soy protein isoelectric point condition, makes a small amount of non-enzymolysis protein be that curdy is separated out;
(7) the solid, liquid composition after enzymolysis is separated:
(6) step is sunk the mixed liquor of process through acid and pumps into centrifugal separator or the Soybean Peptide aqueous solution based on peptide is separated with non-water-soluble solid content by isolated by filtration machine at whipping process, when adopting plate and frame filter press, pressure is 1.0MPa, flow velocity 1000L/h, filter aid is diatomite, filtered solution should reach transparent degree, reserved filtrate, abandons filter cake or give over to other to utilize;
(8) inorganic salts are removed in electrodialysis
It is 2% that the transparent clear liquid that (7) step obtains is added water move to concentration, pump into ion-exchange unit, the acid ion in solution and metal ion is made to be adsorbed in ion-exchange unit respectively, when using electrodialysis plant feed pump pressure be 0.2Mpa, electric current 18A ~ 20A, after solution passes through, the electrical conductivity of material should≤300 μ s/cm
2;
(9) resin is purified and reverse osmosis concentration
The electrical conductivity obtained through step (8) electrodialysis desalination is reached 300 μ s/cm
2feed liquid send in nonpolar CAD40 resin column, be highly purified soybean oligopeptide solution by the liquid phase thing of CAD40 resin column, pump into φ≤10
-10m counter-infiltration (RO) device, efflux is pure water, capable of circulation for the production of, trapped fluid is the highly purified soybean oligopeptide concentrate of concentration ≈ 10%;
(10) activated carbon decolorizing, de-taste
(9) step gained soybean oligopeptide concentrate is added activated carbon decolorizing, and activated carbon grain addition is about 2% of concentrate, the concentrate after active carbon adsorption decolouring, then filters through plate and frame filter press, can obtain colourless underflow liquid;
(11) spraying dry
Colourless, free from extraneous odour, the clear solution that (10) step are obtained, through φ≤10
-7send into after micropore (MF) filters in press spray drying tower, the inlet temperature of tower is 170 DEG C, outlet temperature is 80 DEG C, products obtained therefrom after the spray-dried tower process of feed liquid, is " free from extraneous odour, the highly purified soybean oligopeptide powder " of peptide component content>=80% of free from extraneous odour, peptide purity>=90%, relative molecular mass≤1000.
Embodiment 3 take dregs of beans as the method three that raw material extracts " free from extraneous odour, highly purified soybean oligopeptide ":
(1) remove in dregs of beans in peculiar smell and present-color material:
Fat is remained≤2.5%, protein content >=50%, NSI value >=80% bean cake powder be broken into 60 object meal, send in the pot for solvent extraction of belt stirrer, add again concentration 55% ~ 60% ethanol water, addition is that dregs of rice material weighs 7 times, temperature is kept to be 50 DEG C, mixing speed 30r/min, mixing time 8h;
(2) soybean protein and colour generation, extract respectively in peculiar smell thing:
To complete (1) step adds in the mixed liquor feeding centrifugal separator of ethanol dissolving, under rotating speed about 4000r/min condition, carry out centrifugation, the liquid phase thing flowed out is the solution containing the soybean complex function factor, and being separated and retaining Main Ingredients and Appearance is alcohol insoluble protein (in butt protein content >=65%) and a small amount of solid formation for polymeric carbohydrates such as non-prolamine celluloses;
(3) recycling design ethanol:
(2) step is separating obtained based in the humidity material of alcohol insoluble protein feeding vacuum desiccator through seperator, the vacuum of vacuum desiccator is 77.3kpa, temperature is 70-90 DEG C, processing time 1 ~ 1.5h, feed liquid is concentrated into concentration about 20%, be recycled in ethanol storage tank through vacuum desiccator gained ethanol evaporation again, continue to be cycled to used in production.
(4) " flavor protease " enzymolysis:
By (3) step gained feed liquid, send in the enzymatic vessel of belt stirrer, be diluted with water, dilution dry substance concentration is about 5%, stir while add waterside, mixing speed 30r/min, after stirring, add " flavor protease ", enzyme concentration is 1% ~ 5% of total solid, and water temperature keeps 55 DEG C, and pH keeps 7.2, the enzyme reaction time is 3h, and in this step enzymolysis gained mixed liquor, Main Ingredients and Appearance is the peptide solution in solution state;
(5) albumen and other water-insoluble solid content of non-enzymolysis is removed:
(4) step gained do not had raw meat, smelly, the enzymolysis protein mixed liquor rapid temperature increases of waiting bitterly for peculiar smell to >=80 DEG C, heats 0.5h, carry out enzyme deactivation process;
(6) " acid is heavy " separates out non-enzymolysis protein:
Pump in sour deep gouge by (5) step gained enzyme deactivation mixed liquor, add food grade hydrochloric acid, acid adding limit, limit is stirred, mixing speed is 30 ~ 60r/min, mixed liquor pH value is adjusted to 4.2 ~ 4.5, under soy protein isoelectric point condition, makes a small amount of non-enzymolysis protein be that curdy is separated out;
(7) the solid, liquid composition after enzymolysis is separated:
(6) step is sunk the mixed liquor of process through acid and pumps into centrifugal separator or the Soybean Peptide aqueous solution based on peptide is separated with non-water-soluble solid content by isolated by filtration machine at whipping process, when adopting plate and frame filter press, pressure is 0.1 ~ 1.0MPa, flow velocity 1000L/h, filter aid is diatomite, filtered solution should reach transparent degree, reserved filtrate, abandons filter cake or give over to other to utilize;
(8) inorganic salts are removed in electrodialysis
It is 2% that the transparent clear liquid that (7) step obtains is added water move to concentration, pump into ion-exchange unit, the acid ion in solution and metal ion is made to be adsorbed in ion-exchange unit respectively, when using electrodialysis plant feed pump pressure be 0.2Mpa, electric current 18A ~ 20A, after solution passes through, the electrical conductivity of material should≤300 μ s/cm
2;
(9) resin is purified and reverse osmosis concentration
The electrical conductivity obtained through step (8) electrodialysis desalination is reached 100 ~ 300 μ s/cm
2feed liquid send in nonpolar CAD40 resin column, be highly purified soybean oligopeptide solution by the liquid phase thing of CAD40 resin column, pump into φ≤10
-10m counter-infiltration (RO) device, efflux is pure water, capable of circulation for the production of, trapped fluid is the highly purified soybean oligopeptide concentrate of concentration ≈ 10%;
(10) activated carbon decolorizing, de-taste
(9) step gained soybean oligopeptide concentrate is added activated carbon decolorizing, and activated carbon grain addition is about 2% of concentrate, the concentrate after active carbon adsorption decolouring, then filters through plate and frame filter press, can obtain colourless underflow liquid;
(11) spraying dry
Colourless, free from extraneous odour, the clear solution that (10) step are obtained, through φ≤10
-7send into after micropore (MF) filters in press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, products obtained therefrom after the spray-dried tower process of feed liquid, is " free from extraneous odour, the highly purified soybean oligopeptide powder " of peptide component content>=80% of free from extraneous odour, peptide purity>=90%, relative molecular mass≤1000.
Embodiment 4 is that raw material extracts one of the method for " free from extraneous odour, highly purified soybean oligopeptide " with soybean protein isolate.
(1) soluble protein
Dropped into by soybean protein isolate in the dissolving tank of belt stirrer, input limit, limit is stirred, and mixing speed is 45r/min, and protein isolate and water ratio are 5:95, and water temperature remains on 55 DEG C, stirs 80min;
(2) flavor protease is added, hydrolytic soya bean protein
Add flavor protease in step (1) process, mixing material in dissolved state, enzyme concentration is 4% of protein isolate weight, and coolant-temperature gage remains on 55 DEG C, continues to stir 5h;
(3) sterilising and enzyme inactivating
To complete the protein liquid of enzymolysis through (2) step, heat, heating-up temperature is 90 DEG C, stirs simultaneously, and mixing time is about 20min;
(4) filter, purify
Enzymolysis protein liquid through step (3) is cooled to less than 40 DEG C, adds food grade hydrochloric acid and be adjusted to soy protein isoelectric point pH4.2 ~ 4.5, stir the soybean protein precipitating making non-enzymolysis under 40r ~ 60r/min condition; Again by through waiting mixed liquor of the heavy process of electricity acid to send into flame filter press or filter centrifugal, when using flame filter press, pressure is 0.5MPa, flow velocity is 1000L/h, filter aid is diatomite, mixed liquor is separated by filtration or the liquid phase thing (enzymatic hydrolysis of soybean peptide liquid) of centrifugation gained all to reach transparent level for degree, reserved filtrate, abandons the solid content that retains or transfers to feed or fertilizer;
(5) electrodialysis desalination
The colourless transparent liquid (4) step obtained sends into electrodialysis plant, and feed pump pressure is 0.2MPa, and electrodialysis plant electric current is 18A ~ 20A, and after desalination, the electrical conductivity of material answers 100 ~ 300 μ s/cm
2;
(6) resin is purified
Electrical conductivity after (5) step desalination is answered 100 ~ 300 μ s/cm
2, light brown yellow liquid pumps in non-polar resin column device, abandon the organic matter of resin adsorption or transfer to his and use.
(7) reverse osmosis concentration
The liquid that (6) step flows out after resin adsorption is pumped into φ≤10
-10reverse osmosis unit (RO), by reverse-osmosis treated, efflux is pure water, can use at workshop Inner eycle, after reverse-osmosis treated, take Soybean Peptide as the solution concentration 10% of Main Ingredients and Appearance;
(8) activated carbon decolorizing
(7) step gained liquid is pumped into the bleacher of belt stirrer, add active carbon by 2% of amount of solution, stir 20 ~ 30min, then the solution being mixed with active carbon is sent into plate and frame filter press, at pressure≤3.5Kg/cm
2condition circulating filtration, be colourless to filtrate, reserved filtrate, abandons filter cake or transfers to fertilizer, feed;
(9) spraying dry is finished product
Be the slurries of Soybean Peptide by the solute of (8) step gained, through φ≤10
-7after micropore (MF) filters, gained filtrate sends into press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, the lily powder of the dry gained of the spray-dried tower of feed liquid, for the composition of relative molecular mass≤1000 distributes>=80%, composition total content>=90% of peptide, " free from extraneous odour, the highly purified soybean oligopeptide powder " of free from extraneous odour.
Embodiment 5 take soybean protein isolate as the method two that raw material extracts " free from extraneous odour, highly purified soybean oligopeptide ".
(1) soluble protein
Dropped into by soybean protein isolate in the dissolving tank of belt stirrer, input limit, limit is stirred, and mixing speed is 60r/min, and protein isolate and water ratio are 5:90, and water temperature remains on about 60 DEG C, stirs 30min;
(2) flavor protease is added, hydrolytic soya bean protein
Add flavor protease in step (1) process, mixing material in dissolved state, enzyme concentration is 1% of protein isolate weight, and coolant-temperature gage remains on 60 DEG C, continues to stir 4h;
(3) sterilising and enzyme inactivating
To complete the protein liquid of enzymolysis through (2) step, heat, heating-up temperature is 80 DEG C, stirs simultaneously, and mixing time is about 40min;
(4) filter, purify
Enzymolysis protein liquid through step (3) is cooled to less than 40 DEG C, adds food grade hydrochloric acid and be adjusted to soy protein isoelectric point pH4.2 ~ 4.5, stir the soybean protein precipitating making non-enzymolysis under 40r ~ 60r/min condition; Again by through waiting mixed liquor of the heavy process of electricity acid to send into flame filter press or filter centrifugal, when using flame filter press, pressure is 0.1MPa, flow velocity is 1000L/h, filter aid is diatomite, mixed liquor is separated by filtration or the liquid phase thing (enzymatic hydrolysis of soybean peptide liquid) of centrifugation gained all to reach transparent level for degree, reserved filtrate, abandons the solid content that retains or transfers to feed or fertilizer;
(5) electrodialysis desalination
The colourless transparent liquid (4) step obtained sends into electrodialysis plant, and feed pump pressure is 0.2MPa, and electrodialysis plant electric current is 18A ~ 20A, and after desalination, the electrical conductivity of material answers 100 ~ 300 μ s/cm
2;
(6) resin is purified
Electrical conductivity after (5) step desalination is answered 100 ~ 300 μ s/cm
2, light brown yellow liquid pumps in non-polar resin column device, abandon the organic matter of resin adsorption or transfer to his and use.
(7) reverse osmosis concentration
The liquid that (6) step flows out after resin adsorption is pumped into φ≤10
-10reverse osmosis unit (RO), by reverse-osmosis treated, efflux is pure water, can use at workshop Inner eycle, after reverse-osmosis treated, take Soybean Peptide as the solution concentration 12% of Main Ingredients and Appearance;
(8) activated carbon decolorizing
(7) step gained liquid is pumped into the bleacher of belt stirrer, add active carbon by 2% of amount of solution, stir 20 ~ 30min, then the solution being mixed with active carbon is sent into plate and frame filter press, at pressure≤3.5Kg/cm
2condition circulating filtration, be colourless to filtrate, reserved filtrate, abandons filter cake or transfers to fertilizer, feed;
(9) spraying dry is finished product
Be the slurries of Soybean Peptide by the solute of (8) step gained, through φ≤10
-7after micropore (MF) filters, gained filtrate sends into press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, the lily powder of the dry gained of the spray-dried tower of feed liquid, for the composition of relative molecular mass≤1000 distributes>=80%, composition total content>=90% of peptide, " free from extraneous odour, the highly purified soybean oligopeptide powder " of free from extraneous odour.
Embodiment 6 take soybean protein isolate as the method three that raw material extracts " free from extraneous odour, highly purified soybean oligopeptide ".
(1) soluble protein
Dropped into by soybean protein isolate in the dissolving tank of belt stirrer, input limit, limit is stirred, and mixing speed is 30/min, and protein isolate and water ratio are 5:100, and water temperature remains on about 50 DEG C, stirs 120min;
(2) flavor protease is added, hydrolytic soya bean protein
Add flavor protease in step (1) process, mixing material in dissolved state, enzyme concentration is 5% of protein isolate weight, and coolant-temperature gage remains on 50 DEG C, continues to stir 6h;
(3) sterilising and enzyme inactivating
To complete the protein liquid of enzymolysis through (2) step, heat, heating-up temperature is 85 DEG C, stirs simultaneously, and mixing time is about 30min;
(4) filter, purify
Enzymolysis protein liquid through step (3) is cooled to less than 40 DEG C, adds food grade hydrochloric acid and be adjusted to soy protein isoelectric point pH4.2 ~ 4.5, stir the soybean protein precipitating making non-enzymolysis under 40r ~ 60r/min condition; Again by through waiting mixed liquor of the heavy process of electricity acid to send into flame filter press or filter centrifugal, when using flame filter press, pressure is 1.0MPa, flow velocity is 1000L/h, filter aid is diatomite, mixed liquor is separated by filtration or the liquid phase thing (enzymatic hydrolysis of soybean peptide liquid) of centrifugation gained all to reach transparent level for degree, reserved filtrate, abandons the solid content that retains or transfers to feed or fertilizer;
(5) electrodialysis desalination
The colourless transparent liquid (4) step obtained sends into electrodialysis plant, and feed pump pressure is 0.2MPa, and electrodialysis plant electric current is 18A ~ 20A, and after desalination, the electrical conductivity of material answers 100 ~ 300 μ s/cm
2;
(6) resin is purified
Electrical conductivity after (5) step desalination is answered 100 ~ 300 μ s/cm
2, light brown yellow liquid pumps in non-polar resin column device, abandon the organic matter of resin adsorption or transfer to his and use.
(7) reverse osmosis concentration
The liquid that (6) step flows out after resin adsorption is pumped into φ≤10
-10reverse osmosis unit (RO), by reverse-osmosis treated, efflux is pure water, can use at workshop Inner eycle, after reverse-osmosis treated, take Soybean Peptide as the solution concentration 8% of Main Ingredients and Appearance;
(8) activated carbon decolorizing
(7) step gained liquid is pumped into the bleacher of belt stirrer, add active carbon by 2% of amount of solution, stir 20 ~ 30min, then the solution being mixed with active carbon is sent into plate and frame filter press, at pressure≤3.5Kg/cm
2condition circulating filtration, be colourless to filtrate, reserved filtrate, abandons filter cake or transfers to fertilizer, feed;
(9) spraying dry is finished product
Be the slurries of Soybean Peptide by the solute of (8) step gained, through φ≤10
-7after micropore (MF) filters, gained filtrate sends into press spray drying tower, the inlet temperature of tower is 170 DEG C, outlet temperature is 80 DEG C, the lily powder of the dry gained of the spray-dried tower of feed liquid, for the composition of relative molecular mass≤1000 distributes>=80%, composition total content>=90% of peptide, " free from extraneous odour, the highly purified soybean oligopeptide powder " of free from extraneous odour.
Claims (2)
1. be the method that raw material produces free from extraneous odour, highly purified soybean oligopeptide with dregs of beans, it is characterized in that comprising the following steps:
(1) remove in peculiar smell and present-color material:
Fat is remained≤2.5%, protein content >=50%, NSI value >=80% bean cake powder be broken into 40-60 object meal, send in the pot for solvent extraction of belt stirrer, add again concentration 55% ~ 60% ethanol water, addition is that dregs of rice material weighs 7 ~ 10 times, temperature is kept to be 50 DEG C ~ 60 DEG C, mixing speed 30 ~ 60r/min, mixing time 2 ~ 8h;
(2) soybean protein and colour generation, extract respectively in peculiar smell thing:
To complete (1) step adds in the mixed liquor feeding centrifugal separator of ethanol dissolving, under rotating speed about 3500-4500r/min condition, carry out centrifugation, the liquid phase thing flowed out is the solution containing the soybean complex function factor, being separated and retaining Main Ingredients and Appearance is alcohol insoluble protein (in butt protein content >=65%) and a small amount of solid formation for polymeric carbohydrates such as non-prolamine celluloses, has abandoned peculiar smell, coloured liquid phase thing or has retained deep processing and extracted byproduct;
(3) recycling design ethanol:
(2) step is separating obtained based in the humidity material of alcohol insoluble protein feeding vacuum desiccator through seperator, the vacuum of vacuum desiccator is 77.3kpa, temperature is 70-90 DEG C, processing time 1 ~ 1.5h, be recycled in ethanol storage tank through vacuum desiccator gained ethanol evaporation, continue to be cycled to used in production;
(4) " flavor protease " enzymolysis:
By the mixed liquor that (3) step gained dry substance concentration is ≈ 20%, send in the enzymatic vessel of belt stirrer, be diluted with water, dilution dry substance concentration is 3-7%, stir while add waterside, mixing speed 30 ~ 60r/min, after stirring, add " flavor protease ", time enzyme-added, mixing speed reduces to 25-35r/min, and enzyme concentration is 1% ~ 5% of total solid, water temperature keeps 50-60 DEG C, pH keeps 7.2, and the enzyme reaction time is 3 ~ 6h, and in this step enzymolysis gained mixed liquor, Main Ingredients and Appearance is the peptide solution in solution state;
(5) albumen and other water-insoluble solid content of non-enzymolysis is removed:
(4) step gained do not had raw meat, smelly, the enzymolysis protein mixed liquor rapid temperature increases of waiting bitterly for peculiar smell to >=80 DEG C, heats 20-40 minute, carry out enzyme deactivation process;
(6) " acid is heavy " separates out non-enzymolysis protein:
Pump in sour deep gouge by (5) step gained enzyme deactivation mixed liquor, add food grade hydrochloric acid, acid adding limit, limit is stirred, mixing speed is 30 ~ 60r/min, mixed liquor pH value is adjusted to 4.2 ~ 4.5, under soy protein isoelectric point condition, makes a small amount of non-enzymolysis protein be that curdy is separated out;
(7) the solid, liquid composition after enzymolysis is separated:
(6) step is sunk the mixed liquor of process through acid and pumps into centrifugal separator or the Soybean Peptide aqueous solution based on peptide is separated with non-water-soluble solid content by isolated by filtration machine at whipping process, when adopting plate and frame filter press, pressure is 0.1 ~ 1.0MPa, flow velocity 800-1200L/h, filter aid is diatomite, filtered solution should reach transparent degree, reserved filtrate, abandons filter cake or transfers to fertilizer, feed;
(8) inorganic salts are removed in electrodialysis
It is 2% that the transparent clear liquid that (7) step obtains is added water move to concentration, pump into ion-exchange unit, the acid ion in solution and metal ion is made to be adsorbed in ion-exchange unit respectively, when using electrodialysis plant feed pump pressure be 0.15-0.25Mpa, electric current 18A ~ 20A, after solution passes through, the electrical conductivity of material should≤300 μ s/cm
2;
(9) resin is purified and reverse osmosis concentration
The electrical conductivity obtained through step (8) electrodialysis desalination is reached 100 ~ 300 μ s/cm
2feed liquid send in nonpolar CAD40 resin column, be highly purified soybean oligopeptide solution by the liquid phase thing of CAD40 resin column, pump into φ≤10
-10m counter-infiltration (RO) device, efflux is pure water, capable of circulation for the production of, trapped fluid is the highly purified soybean oligopeptide concentrate of concentration ≈ 10%;
(10) activated carbon decolorizing, de-taste
(9) step gained soybean oligopeptide concentrate is added activated carbon decolorizing, and activated carbon grain addition is about the 1-3% of concentrate, the concentrate after active carbon adsorption decolouring, then filters through plate and frame filter press, can obtain colourless underflow liquid;
(11) spraying dry
Colourless, free from extraneous odour, the clear solution that (10) step are obtained, through φ≤10
-7send into after micropore (MF) filters in press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, products obtained therefrom after the spray-dried tower process of feed liquid, is " free from extraneous odour, the highly purified soybean oligopeptide powder " of peptide component content>=80% of free from extraneous odour, peptide purity>=90%, relative molecular mass≤1000.
2. be the method that raw material produces free from extraneous odour, highly purified soybean oligopeptide with soybean protein isolate, it is characterized in that comprising the following steps:
(1) soluble protein
Dropped into by soybean protein isolate in the dissolving tank of belt stirrer, input limit, limit is stirred, and mixing speed is 30 ~ 60r/min, and protein isolate and water ratio are 5:90 ~ 100, and water temperature remains between 50 ~ 60 DEG C, stirs 30 ~ 120min;
(2) flavor protease is added, hydrolytic soya bean protein
Add flavor protease in step (1) process, mixing material in dissolved state, enzyme concentration is 1% ~ 5% of protein isolate weight, and coolant-temperature gage remains between 50 ~ 60 DEG C, continues stirring 4 ~ 6h;
(3) sterilising and enzyme inactivating
To complete the protein liquid of enzymolysis through (2) step, heat, heating-up temperature is 80 ~ 90 DEG C, stirs simultaneously, mixing time 20-40min;
(4) filter, purify
Enzymolysis protein liquid through step (3) is cooled to less than 40 DEG C, adds food grade hydrochloric acid and be adjusted to soy protein isoelectric point pH4.2 ~ 4.5, stir the soybean protein precipitating making non-enzymolysis under 40r ~ 60r/min condition; Again by through waiting mixed liquor of the heavy process of electricity acid to send into flame filter press or filter centrifugal, when using flame filter press, pressure is 0.1 ~ 1.0MPa, flow velocity is 1000L/h, filter aid is diatomite, mixed liquor is separated by filtration or the liquid phase thing (enzymatic hydrolysis of soybean peptide liquid) of centrifugation gained all to reach transparent level for degree, reserved filtrate, abandons the solid formation that retains or transfers to feed or fertilizer;
(5) electrodialysis desalination
The colourless transparent liquid (4) step obtained sends into electrodialysis plant, and feed pump pressure is 0.15-0.25MPa, and electrodialysis plant electric current is 18A ~ 20A, and after desalination, the electrical conductivity of material answers 100 ~ 300 μ s/cm
2;
(6) resin is purified
Electrical conductivity after (5) step desalination is answered 100 ~ 300 μ s/cm
2, light brown yellow liquid pumps in non-polar resin column device, abandon resin adsorption thing or reserve for other use as byproduct;
(7) reverse osmosis concentration
The liquid that (6) step flows out after resin adsorption is pumped into φ≤10
-10reverse osmosis unit (RO), by reverse-osmosis treated, efflux is pure water, can use at workshop Inner eycle, after reverse-osmosis treated, take Soybean Peptide as the solution concentration 8-12% of Main Ingredients and Appearance;
(8) activated carbon decolorizing
(7) step gained liquid is pumped into the bleacher of belt stirrer, add active carbon by 2% of amount of solution, stir 20 ~ 30min, then the solution being mixed with active carbon is sent into plate and frame filter press, at pressure≤3.5Kg/cm
2condition circulating filtration, be colourless to filtrate, reserved filtrate, abandon filter cake or as byproduct he use;
(9) spraying dry is finished product
Be the slurries of Soybean Peptide by the solute of (8) step gained, through φ≤10
-7after micropore (MF) filters, gained filtrate sends into press spray drying tower, the inlet temperature of tower is 170 ° ~ 180 DEG C, outlet temperature is 70 ° ~ 80 DEG C, the lily powder of the dry gained of the spray-dried tower of feed liquid, for the composition of relative molecular mass≤1000 distributes>=80%, composition total content>=90% of peptide, " free from extraneous odour, the highly purified soybean oligopeptide powder " of free from extraneous odour.
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| WO2016172979A1 (en) * | 2015-04-30 | 2016-11-03 | 中国食品发酵工业研究院 | Hypoallergenic, bitterness-reduced soybean oligopeptide, preparation method for same, and applications thereof |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN111034851A (en) * | 2018-10-15 | 2020-04-21 | 徐国庆 | Production method capable of improving purity and yield of soybean peptide |
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| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
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| CN111394413A (en) * | 2020-05-18 | 2020-07-10 | 浙江龙游李子园食品有限公司 | Method for extracting soybean peptide from bean dregs which is byproduct of soybean milk processing |
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