CN108208309A - The production method of low ash content soybean peptide containing profitable probliotics - Google Patents
The production method of low ash content soybean peptide containing profitable probliotics Download PDFInfo
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- CN108208309A CN108208309A CN201711463672.6A CN201711463672A CN108208309A CN 108208309 A CN108208309 A CN 108208309A CN 201711463672 A CN201711463672 A CN 201711463672A CN 108208309 A CN108208309 A CN 108208309A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 75
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 74
- 244000068988 Glycine max Species 0.000 title claims abstract description 73
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 70
- 238000000034 method Methods 0.000 claims abstract description 40
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 238000000909 electrodialysis Methods 0.000 claims abstract description 20
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 238000001728 nano-filtration Methods 0.000 claims abstract description 19
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 19
- 238000005342 ion exchange Methods 0.000 claims abstract description 12
- 238000001471 micro-filtration Methods 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 239000006041 probiotic Substances 0.000 claims abstract description 11
- 235000018291 probiotics Nutrition 0.000 claims abstract description 11
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 9
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 6
- 239000003531 protein hydrolysate Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 108091005804 Peptidases Proteins 0.000 claims description 11
- 239000004365 Protease Substances 0.000 claims description 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 11
- 239000007921 spray Substances 0.000 claims description 10
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 9
- 239000003729 cation exchange resin Substances 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 239000000796 flavoring agent Substances 0.000 claims description 8
- 235000019634 flavors Nutrition 0.000 claims description 8
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 5
- 230000000996 additive effect Effects 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 239000003957 anion exchange resin Substances 0.000 claims description 5
- 238000010612 desalination reaction Methods 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 2
- 239000002956 ash Substances 0.000 abstract description 49
- 239000000047 product Substances 0.000 abstract description 16
- 235000002918 Fraxinus excelsior Nutrition 0.000 abstract description 10
- 235000019658 bitter taste Nutrition 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 235000019606 astringent taste Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000019640 taste Nutrition 0.000 description 3
- 238000002834 transmittance Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 235000013597 soy food Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to the production methods of the low ash content soybean peptide containing profitable probliotics, include the following steps：(1) enzyme digestion reaction is carried out to soybean protein isolate, prepares soybean peptide reaction solution；(2) soybean peptide reaction solution is removed into non-protein hydrolysate through micro-filtration, micro-filtrate is made, then through decoloration, feed liquid after decoloration is made；(3) feed liquid after nanofiltration is made through nanofiltration in feed liquid after decolourizing；(4) feed liquid after electrodialysis process is made through homogeneous membrane electrodialysis process in feed liquid after nanofiltration；(5) low ash content soybean peptide primary liquid is made through ion exchange in feed liquid after electrodialysis process；(6) it is low ash content soybean peptide primary liquid is concentrated, probiotics is added in, it is dry, the low ash content soybean peptide containing profitable probliotics is made.The first public content of ashes of the present invention can significantly affect the mouthfeel of soybean peptide, and the mouthfeel of product is made to be significantly improved.
Description
Technical field
The present invention relates to the production methods of the low ash content soybean peptide containing profitable probliotics, belong to soybean protein deep process technology neck
Domain.
Background technology
Soybean peptide is by the soybean protein of macromolecular, utilizes small molecule segment made of biotechnology.Soybean peptide is small
The protein of molecule is very easy to be absorbed by the body, and protein digestibility is suitble to absorb bad crowd and is eaten, in addition, soybean peptide
Also have and improve immunity, enhancing muscle power, fatigue-relieving, lower hyperlipidemia, hypertension, hyperglycemia and other effects, be excellent food ingredient, be widely used in eating
The fields such as product, health food, infant food, sports food, fermented product and clinical nutritive preparations.
At present, the method for common preparation soybean peptide has the joint of enzyme hydrolysis method, microbe fermentation method and two methods
It uses.
Soybean peptide prepared by existing patent or patent application controls height, typically less than for the ash content of soybean peptide
7%, soybean peptide product on the market is primarily present bitter highly seasoned, influences the application field of product.
Chinese patent literature CN101491288A (application number 200910064077.4) discloses a kind of microbial fermentation system
Standby soybean peptide technique.Its step of preparation process：By 2%~10% soybean protein isolate, 0.1%~2% sodium chloride,
0.1%~2% potassium dihydrogen phosphate and drinking water mixing, production protease microorganism 25 DEG C of the rear disinfection inoculation through independent research~
40 DEG C ferment, and fermentation 12~for 24 hours, obtain zymotic fluid；Centrifuge zymotic fluid, filtering use the penetrating amount of molecule for 2000~
The cellulose membrane of 10000Dalton takes off bitter deodorization with active carbon adsorption column, is spray-dried after vacuum concentration, obtains soy peptide powder.This
Invention prepares that soybean peptide is simple for process, and the time is short, fermentation period for 12~for 24 hours, shorten 24~36h than general fermentation time 48h,
It is at low cost；Primary raw material soybean protein isolate derives from a wealth of sources；The soybean peptide gross protein 99.1% of preparation, soybean peptide 82.2%,
Ash content 6.3%, for 89.0% soybean peptide molecular weight less than 2000Dalton, quality is better than QB/T2653I type indexs, has potential
The small peptide of bioactivity accounts for the overwhelming majority, small compared with soybean peptide bitter taste prepared by enzymatic isolation method, mouthfeel, raciness.
But product made from the above method still has bitter taste and other tastes beastly, and in addition probiotics
Afterwards, which can be more obvious.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of production methods of the low ash content soybean peptide containing profitable probliotics.
This method is using soybean protein isolate as raw material, by enzymolysis, micro-filtration, decoloration, nanofiltration, homogeneous membrane electrodialysis, ion exchange, concentration
It mixing bacterium powder, drying etc. and the low ash content soybean peptide containing profitable probliotics is made, final products are more than 2,000,000,000/gram containing profitable probliotics,
Ash content is less than 1%, and completely eliminated the bitter taste and offensive odour of soybean peptide, even if add after probiotics not
It can lead to the above-mentioned smell of consumers' perceptions.
Technical scheme is as follows：
The production method of low ash content soybean peptide containing profitable probliotics, includes the following steps：
(1) enzyme digestion reaction is carried out to soybean protein isolate, prepares soybean peptide reaction solution；
(2) soybean peptide reaction solution made from step (1) is removed into non-protein hydrolysate through micro-filtration, micro-filtrate, Ran Houjing is made
Feed liquid after decoloration is made in decoloration；
(3) feed liquid after step (2) decoloration obtained is made through the NF membrane nanofiltration that molecular cut off is 200 dalton
Feed liquid after nanofiltration；
The μ s/cm of feed liquid conductivity after the step process≤300；
(4) by feed liquid after nanofiltration made from step (3) through homogeneous membrane electrodialysis process, desalination solution and concentrated water ratio for (8~
12):Feed liquid after electrodialysis process is made in 1,5~8A of electric current, 25~35V of voltage；
The μ s/cm of feed liquid conductivity after the step process≤100；
(5) by feed liquid after electrodialysis process made from step (4) through ion exchange, 30~35 DEG C of feed temperature, ion-exchange into
It is 8~10% to expect mass concentration, and feed liquid is introduced into cation exchange resin, subsequently into anion exchange resin, feed flow rate
For 2~3 times of resin volumes per hour, discharge pH4~6, and low ash content soybean peptide primary liquid is made；
The μ s/cm of feed liquid conductivity after the step process≤50；
(6) it is ash content soybean peptide primary liquid low made from step (5) is concentrated, probiotics is then added in, through drying, is made
Low ash content soybean peptide containing profitable probliotics.
According to currently preferred, the enzyme digestion reaction in the step (1), step is：Soybean protein isolate plus water are matched
The solution that mass concentration is 8~10% is made, then adjusts pH7.5~8.5,55~60 DEG C of temperature, adds in by soybean protein isolate
0.8~1.5% ratio of dry weight added in alkali protease and 0.4~0.6% flavor protease, through 6~8 hours enzymes
Solution reaction after, enzyme deactivation work to get.
According to the present invention it is further preferred that above-mentioned enzyme deactivation is lived to adjust pH 4.0~4.5.
According to currently preferred, in the step (2), the aperture of micro-filtration is 1 μm.
According to currently preferred, in the step (2), decolourize for by mass percentage 1% ratio into micro-filtrate
Activated carbon is added in, under conditions of 55~65 DEG C of temperature, pH4~6, decolourize 90~120min, and feed liquid after decoloration is made.
According to currently preferred, in the step (4), the electrodialytic pole water of homogeneous membrane is mass concentration 1.5~2.5%
Sodium chloride solution.
According to currently preferred, in the step (6), probiotics is streptococcus thermophilus.
It is dry using spray drying band fluid bed equipment integrating drying in the step (6) according to currently preferred,
Wherein 150~160 DEG C of the inlet air temperature of spray tower, 70~85 DEG C of leaving air temp spray Ta Nei -0.06~-0.04mPa, fluid bed
48~52 DEG C of inlet temperature, 28~32 DEG C of outlet temperature, 9~11 minutes time.
According to currently preferred, in the step (6), it is 30~35% to be concentrated into mass concentration.
According to currently preferred, in the step (6), the additive amount of probiotics is every gram of low ash content soybean peptide concentrate
Dry weight be no less than 2,000,000,000.
Advantageous effect
1st, inventor has found that content of ashes can significantly affect the mouthfeel of soybean peptide, when content of ashes height for the first time by research
After 1%, the bitter taste of soybean peptide and taste beastly can be dramatically increased, and when content of ashes is down to below 1%,
Above-mentioned smell will disappear, and so as to the soybean peptide for being less than 1% by preparing content of ashes, the mouthfeel of product be made significantly to be carried
It rises；
2nd, inventor is adding probiotics into soybean peptide, contributes to during human consumption soybean peptide, while edible benefit
Raw bacterium helps to improve the intestinal health of eater.
Specific embodiment
With reference to embodiment, technical scheme of the present invention is further elaborated, this is to be better understood from this hair
It is bright, but institute's protection domain of the present invention is without being limited thereto.
Raw material sources：
Soybean protein isolate is purchased from Kazakhstan high-tech soy food product company；Composition detection thick protein 91.1%, ash content 4.4%,
Moisture 4.93%, fat 0.3%；
Streptococcus thermophilus is purchased from Beijing Chuan Xiu Science and Technology Ltd.s.
Embodiment 1
The production method of low ash content soybean peptide containing profitable probliotics, includes the following steps：
(1) water is added to be configured to the solution that mass concentration is 10% soybean protein isolate, then adjusts pH8.0,55 DEG C of temperature,
It adds in and adds in alkali protease and 0.5% flavor protease in 1.0% ratio of soybean protein isolate dry weight, through 6
After hour enzyme digestion reaction, pH4.0 is adjusted, prepares soybean peptide reaction solution；
(2) by the micro-filtration membrane microfiltration that soybean peptide reaction solution via hole diameter made from step (1) is 1 μm, non-protein hydrolysate is removed,
Micro-filtrate is made, then 1% ratio adds in activated carbon into micro-filtrate by mass percentage, in 55 DEG C of temperature, the item of pH4.2
Under part, decolourize 90min, and feed liquid after decoloration, after testing, feed liquid light transmittance 85% is made；
(3) feed liquid after step (2) decoloration obtained is made through the NF membrane nanofiltration that molecular cut off is 200 dalton
Feed liquid after nanofiltration；
The μ s/cm of feed liquid conductivity after the step process≤300；
(4) by feed liquid after nanofiltration made from step (3) through homogeneous membrane electrodialysis process, desalination solution and concentrated water ratio are 10:1,
It is pole water with sodium chloride solution, feed liquid after electrodialysis process is made in electric current 8A, voltage 30V；
The μ s/cm of feed liquid conductivity after the step process≤100；
(5) by feed liquid after electrodialysis process made from step (4) through ion exchange, 32 DEG C of feed temperature, ion-exchange charging matter
A concentration of 8% is measured, by anion-cation exchange resin, feed liquid is introduced into cation exchange resin, subsequently into anion exchange
Resin, feed flow rate are 2 times of resin volumes per hour, and low ash content soybean peptide primary liquid is made；
The μ s/cm of feed liquid conductivity after the step process≤50；
(6) it is 35% ash content soybean peptide primary liquid low made from step (5) to be concentrated into mass concentration, low ash content soybean peptide
Concentrate, adds in streptococcus thermophilus, the additive amount of streptococcus thermophilus for every gram of low ash content soybean peptide concentrate dry weight not
Less than 2,000,000,000, then spray-dried band fluid bed equipment integrating drying, wherein 160 DEG C of the inlet air temperature of spray tower, outlet air
Temperature 70 C, spray Ta Nei -0.05mPa, 50 DEG C of fluid bed inlet temperatures, 30 DEG C of outlet temperature, 10 minutes time are made containing beneficial
The low ash content soybean peptide of raw bacterium.
After testing, content of ashes is 0.5% in the low ash content soybean peptide containing profitable probliotics.
Embodiment 2
The production method of low ash content soybean peptide containing profitable probliotics, includes the following steps：
(1) water is added to be configured to the solution that mass concentration is 8% soybean protein isolate, then adjusts pH7.5,55 DEG C of temperature,
It adds in and adds in alkali protease and 0.6% flavor protease in 0.8% ratio of soybean protein isolate dry weight, through 8
After hour enzyme digestion reaction, pH 4.5 is adjusted, prepares soybean peptide reaction solution；
(2) by the micro-filtration membrane microfiltration that soybean peptide reaction solution via hole diameter made from step (1) is 1 μm, non-protein hydrolysate is removed,
Micro-filtrate is made, then 1% ratio adds in activated carbon into micro-filtrate by mass percentage, in temperature 60 C, the item of pH4.5
Under part, decolourize 120min, and feed liquid after decoloration, after testing, feed liquid light transmittance 90% is made；
(3) feed liquid after step (2) decoloration obtained is made through the NF membrane nanofiltration that molecular cut off is 200 dalton
Feed liquid after nanofiltration；
The μ s/cm of feed liquid conductivity after the step process≤300；
(4) by feed liquid after nanofiltration made from step (3) through homogeneous membrane electrodialysis process, desalination solution and concentrated water ratio are 10:1,
It is pole water with sodium chloride solution, feed liquid after electrodialysis process is made in electric current 6A, voltage 30V；
The μ s/cm of feed liquid conductivity after the step process≤100；
(5) by feed liquid after electrodialysis process made from step (4) through ion exchange, 30 DEG C of feed temperature, ion-exchange charging matter
A concentration of 7% is measured, by anion-cation exchange resin, feed liquid is introduced into cation exchange resin, subsequently into anion exchange
Resin, feed flow rate are 3 times of resin volumes per hour, and low ash content soybean peptide primary liquid is made；
The μ s/cm of feed liquid conductivity after the step process≤50；
(6) it is 30% ash content soybean peptide primary liquid low made from step (5) to be concentrated into mass concentration, low ash content soybean peptide
Concentrate, adds in streptococcus thermophilus, the additive amount of streptococcus thermophilus for every gram of low ash content soybean peptide concentrate dry weight not
Less than 2,000,000,000, then through then spray-dried band fluid bed equipment integrating drying, the wherein inlet air temperature 150 of spray tower
DEG C, 70-85 DEG C of leaving air temp, spray Ta Nei -0.05mPa, 50 DEG C of fluid bed inlet temperatures, 30 DEG C of outlet temperature, 10 minutes time,
The low ash content soybean peptide containing profitable probliotics is made.
After testing, content of ashes is 0.3% in the low ash content soybean peptide containing profitable probliotics.
Embodiment 3
The production method of low ash content soybean peptide containing profitable probliotics, includes the following steps：
(1) water is added to be configured to the solution that mass concentration is 10% soybean protein isolate, then adjusts pH8.5, temperature 60 C,
It adds in and adds in alkali protease and 0.4% flavor protease in 1.5% ratio of soybean protein isolate dry weight, through 7
After hour enzyme digestion reaction, pH 4.2 is adjusted, prepares soybean peptide reaction solution；
(2) by the micro-filtration membrane microfiltration that soybean peptide reaction solution via hole diameter made from step (1) is 1 μm, non-protein hydrolysate is removed,
Micro-filtrate is made, then 1% ratio adds in activated carbon into micro-filtrate by mass percentage, in the condition of temperature 60 C, pH5
Under, decolourize 100min, and feed liquid after decoloration, after testing, feed liquid light transmittance 87% is made；
(3) feed liquid after step (2) decoloration obtained is made through the NF membrane nanofiltration that molecular cut off is 200 dalton
Feed liquid after nanofiltration；
The μ s/cm of feed liquid conductivity after the step process≤300；
(4) by feed liquid after nanofiltration made from step (3) through homogeneous membrane electrodialysis process, desalination solution and concentrated water ratio are 10:1,
It is pole water with sodium chloride solution, feed liquid after electrodialysis process is made in electric current 5A, voltage 30V；
The μ s/cm of feed liquid conductivity after the step process≤100；
(5) by feed liquid after electrodialysis process made from step (4) through ion exchange, 35 DEG C of feed temperature, ion-exchange charging matter
A concentration of 9% is measured, by anion-cation exchange resin, feed liquid is introduced into cation exchange resin, subsequently into anion exchange
Resin, feed flow rate are 2 times of resin volumes per hour, and low ash content soybean peptide primary liquid is made；
The μ s/cm of feed liquid conductivity after the step process≤50；
(6) it is 32% ash content soybean peptide primary liquid low made from step (5) to be concentrated into mass concentration, low ash content soybean peptide
Concentrate, adds in streptococcus thermophilus, the additive amount of streptococcus thermophilus for every gram of low ash content soybean peptide concentrate dry weight not
Less than 2,000,000,000, then through then spray-dried band fluid bed equipment integrating drying, the wherein inlet air temperature 155 of spray tower
DEG C, 70-85 DEG C of leaving air temp, spray Ta Nei -0.05mPa, 50 DEG C of fluid bed inlet temperatures, 30 DEG C of outlet temperature, 10 minutes time,
The low ash content soybean peptide containing profitable probliotics is made.
After testing, content of ashes is 0.4% in the low ash content soybean peptide containing profitable probliotics.
Comparative example 1
Preparation method as described in Example 1, the difference lies in without the homogeneous membrane electrodialysis of step (4) and ion-exchange
Resin treatment.
Soybean peptide obtained after testing, content of ashes 4.4%.
Experimental example
Detection method
The detection method of smell is with reference to GB/T20884-2007；
The detection method of flavour is with reference to GB/T20884-2007；
Product made from embodiment 1-3 and comparative example 1 is detected according to the method described above, the results are shown in Table 1.
1 smell of table and the flavour table of comparisons
| Comparative example 1 | Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Smell | Fishy smell weight | Free from extraneous odour | Free from extraneous odour | Free from extraneous odour |
| Flavour | It is bitter highly seasoned | Light sweet tea astringent taste | Light sweet tea astringent taste | Light sweet tea astringent taste |
As seen from Table 1, after the present invention is by targetedly removing the ash content ingredient in product, work as production
When content of ashes in product is less than 1%, the fishy smell in product odour is got rid of, and the bitter taste in product taste eliminates, astringent taste
It is substantially reduced, so as to greatly improve acceptance level of the consumer to product, be conducive to a wide range of popularization and application of product.
Claims (10)
1. the production method of the low ash content soybean peptide containing profitable probliotics, which is characterized in that include the following steps：
(1) enzyme digestion reaction is carried out to soybean protein isolate, prepares soybean peptide reaction solution；
(2) soybean peptide reaction solution made from step (1) is removed into non-protein hydrolysate through micro-filtration, micro-filtrate is made, then through decolourizing,
Feed liquid after decolourizing is made；
(3) nanofiltration is made through the NF membrane nanofiltration that molecular cut off is 200 dalton in feed liquid after step (2) decoloration obtained
Feed liquid afterwards；
(4) by feed liquid after nanofiltration made from step (3) through homogeneous membrane electrodialysis process, desalination solution and concentrated water ratio are (8~12):1,
Feed liquid after electrodialysis process is made in 5~8A of electric current, 25~35V of voltage；
(5) by feed liquid after electrodialysis process made from step (4) through ion exchange, 30~35 DEG C of feed temperature, ion-exchange charging matter
A concentration of 8~10% are measured, feed liquid is introduced into cation exchange resin, and subsequently into anion exchange resin, feed flow rate is every
2~3 times of resin volumes of hour, discharge pH4~6, and low ash content soybean peptide primary liquid is made；
(6) it is ash content soybean peptide primary liquid low made from step (5) is concentrated, probiotics is then added in, through drying, is made and contains
The low ash content soybean peptide of probiotics.
2. production method as described in claim 1, which is characterized in that the enzyme digestion reaction in the step (1), step are：It will
Soybean protein isolate adds water to be configured to the solution that mass concentration is 8~10%, then adjusts pH7.5~8.5,55~60 DEG C of temperature,
It adds in and adds in alkali protease and 0.4~0.6% flavor in 0.8~1.5% ratio of soybean protein isolate dry weight
Protease, after 6~8 hours enzyme digestion reactions, enzyme deactivation work to get.
3. production method as claimed in claim 2, which is characterized in that the enzyme deactivation is lived to adjust pH 4.0~4.5.
4. production method as described in claim 1, which is characterized in that in the step (2), the aperture of micro-filtration is 1 μm.
5. production method as described in claim 1, which is characterized in that in the step (2), decolourize for by mass percentage
1% ratio adds in activated carbon into micro-filtrate, and under conditions of 55~65 DEG C of temperature, pH4~6, decolourize 90~120min, system
Feed liquid after must decolourizing.
6. production method as described in claim 1, which is characterized in that in the step (4), the electrodialytic pole water of homogeneous membrane is
The sodium chloride solution of mass concentration 1.5~2.5%.
7. production method as described in claim 1, which is characterized in that in the step (6), probiotics is streptococcus thermophilus.
8. production method as described in claim 1, which is characterized in that dry using spray drying band stream in the step (6)
Change the drying of bed equipment integrating, wherein 150~160 DEG C of the inlet air temperature of spray tower, 70~85 DEG C of leaving air temp sprays Ta Nei -0.06
~-0.04mPa, 48~52 DEG C of fluid bed inlet temperatures, 28~32 DEG C of outlet temperature, 9~11 minutes time.
9. production method as described in claim 1, which is characterized in that in the step (6), be concentrated into mass concentration for 30~
35%.
10. production method as described in claim 1, which is characterized in that in the step (6), the additive amount of probiotics is every
The dry weight of gram low ash content soybean peptide concentrate is no less than 2,000,000,000.
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