CN103695513A - Method for improving yield of soybean peptide with low molecular weight - Google Patents
Method for improving yield of soybean peptide with low molecular weight Download PDFInfo
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- CN103695513A CN103695513A CN201310478523.2A CN201310478523A CN103695513A CN 103695513 A CN103695513 A CN 103695513A CN 201310478523 A CN201310478523 A CN 201310478523A CN 103695513 A CN103695513 A CN 103695513A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 60
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 56
- 244000068988 Glycine max Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000000243 solution Substances 0.000 claims abstract description 61
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 52
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 40
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 40
- 239000000706 filtrate Substances 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000011259 mixed solution Substances 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 238000001471 micro-filtration Methods 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 238000001728 nano-filtration Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 239000000047 product Substances 0.000 abstract description 8
- 239000003929 acidic solution Substances 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 abstract description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000002244 precipitate Substances 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000007701 flash-distillation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000004280 healthy diet Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for improving yield of soybean peptide with low molecular weight, which employs an enzyme-membrane method for carrying out deep processing of isolated soybean protein, and controls yield of reaction by controlling sugar degree of enzymatic hydrolysis liquid, and controls product yield by controlling sugar degree of filtrate and water addition, and controls product transparency by controlling concentrate sugar degree. The invention has the advantages of high peptide content, clear and transparent solution, high yield rate, low soybean peptide molecular weight and easy absorption, and even in acidic solution, the soy peptides do not precipitate, and can be widely applied in the fields such as foodstuff, cosmetic, etc.
Description
Technical field
The present invention relates to soybean protein deep process technology field, be specifically related to a kind of method that improves lower molecular weight soybean peptides output.
Background technology
Along with the raising day by day of standard of living, people also improve constantly the degree of concern of healthy diet.Mode for picked-up albumen, people are only no longer confined to from panning protein in animal body, and more from healthy angle go out to send picked-up some containing the vegetable-protein of cholesterol, and with respect to other vegetable-protein, soybean protein has the most comprehensive nutritive value.But, because soybean protein exists a lot of limitation in processing, in practical application, conventionally soybean protein is processed into the soybean peptides that can more easily be utilized by people, meanwhile also can not affect original function in soybean protein.
At present, the manufacturing and processing enterprise of existing soybean peptides conventionally adopts following process for processing mode: soybean protein isolate through dissolving, enzymolysis, centrifugation, sterilization, flash distillation deodorization, high temperature is concentrated, spraying is dry, packaging final prod; The soybean peptides molecular weight product that aforesaid method is produced is large, and skewness can form precipitation in acidic solution, and after dilution, solution colour is very dark, the inadequate clear of solution.
Based on above factor, the microfiltration membrane treatment technology that our company adopts is different from traditional centrifugal separation technique, the molecular weight that can guarantee filtrate distributes in certain scope, obtained soybean peptides solution is limpid bright, peptide content is high, and by controlling the pol of filtrate, can effectively improve the output capacity of product
Summary of the invention
The object of this invention is to provide a kind of method that improves lower molecular weight soybean peptides output, not only productive rate is high for present method gained soybean peptides, and soybean peptides molecular weight is lower, and soybean peptides solution is limpid bright, and peptide content is high.
In order to realize above object, the technical solution adopted in the present invention is: a kind of method that improves lower molecular weight soybean peptides output, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 6～11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 5 ‰ of soybean protein isolate weight～40 ‰;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
The enzymolysis solution pol of described step (2) gained is 5～20BX, and pH value is 6～11.
In described step (3), filtrate pol is 4～15BX, and in cleaning process, the weight ratio of water and filtrate is 1:1～1:15.
In described step (3), filtrate pol is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15.
In described step (4), the pol of concentrated solution is 10～35BX.
In described step (4), the pol of concentrated solution is 12BX.
The enzymolysis time of described step (2) soybean protein isolate solution is 2h～10h.
The method of raising lower molecular weight soybean peptides output provided by the present invention, adopted enzyme-embrane method to carry out deep processing to soybean protein isolate, by the pol of controlled enzymatic hydrolysis liquid, control the yield of reaction, by controlling pol and the amount of water of filtrate, control product yield, by control, control concentrated solution pol and control product transparency, not only peptide content is high for the soybean peptides that present method is produced, solution is limpid bright, the output capacity of product is high, and soybean peptides molecular weight is low, easily absorb, even do not precipitate in acidic solution yet, can be widely used in a plurality of fields such as foods and cosmetics.
Embodiment
Below by specific embodiment, technical scheme of the present invention is elaborated.
Embodiment 1
A kind of method that improves lower molecular weight soybean peptides output that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 6;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 5 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 2h, and the pol of enzymolysis solution is 5BX, and pH value is 6;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching; Wherein the pol of gained filtrate is 4BX, and in cleaning process, the weight ratio of water and filtrate is 1:1;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 10BX;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
Embodiment 2
A kind of method that improves lower molecular weight soybean peptides output that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 8;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 20 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 5h, and the pol of enzymolysis solution is 10BX, and pH value is 8;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching; Wherein the pol of gained filtrate is 10BX, and in cleaning process, the weight ratio of water and filtrate is 1:5;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 20BX;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
Embodiment 3
A kind of method that improves lower molecular weight soybean peptides output that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 8;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 30 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 8h, and the pol of enzymolysis solution is 18BX, and pH value is 8;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching; Wherein the pol of gained filtrate is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 12BX;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
Embodiment 4
A kind of method that improves lower molecular weight soybean peptides output that the present embodiment provides, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 40 ‰ of soybean protein isolate weight; Wherein enzymolysis time is 10h, and the pol of enzymolysis solution is 20BX, and pH value is 11;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching; Wherein the pol of gained filtrate is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution; Wherein the pol of the concentrated solution of gained is 35BX;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
In addition, compound protease provided by the present invention is purchased from Yi Xin bio tech ltd, Shanghai.
Test example
According to embodiment 1～4 provided by the present invention, test below, and calculate the soybean peptides productive rate of each embodiment, then after the soybean peptides of each embodiment gained is water-soluble, measure respectively it at the absorbancy at 660nm place, i.e. OD(660nm); In addition, embodiment 1～4 the data obtained is contrasted with the resulting numerical value of method test providing in applied research > > (hereinafter referred to as method 1) paper according to the separated enzymic hydrolysis of < < soybean and peptide, specifically in Table lattice 1.
The correlation data of form 1 the present invention and currently known methods output soybean peptides
From table 1, can see, the soybean peptides productive rate of embodiment 3 provided by the invention is up to 70.31%, far away higher than the soybean peptides productive rate 45.50% of method 1 at present; In addition, the soybean peptides of embodiment 3 gained is water-soluble after, the absorbancy at the 660nm place measuring is also high compared with the absorbancy of the soybean peptides of method 1 gained.Therefore, the soybean peptides molecular weight that the present invention produces is low, easily absorb, and the output capacity of product is high, and gained solution is limpid bright.
Claims (7)
1. a method that improves lower molecular weight soybean peptides output, is characterized in that, comprises the following steps:
(1) take soybean protein isolate, add water, after soybean protein isolate fully dissolves, regulating soybean protein isolate pH value of solution is 6～11;
(2) by after step (1) gained soybean protein isolate solution sterilization, add compound protease to carry out obtaining enzymolysis solution after enzymolysis, conjugated protein enzyme dosage is 5 ‰ of soybean protein isolate weight～40 ‰;
(3) enzymolysis solution of step (2) gained is carried out after microfiltration membrane processing, add water and clean filtrate, and collect the soybean peptides mixed solution leaching;
(4) the soybean peptides mixed solution of step (3) gained is carried out to nanofiltration operation, collect and obtain concentrated solution;
(5) spraying after the concentrated solution sterilizing of step (4) gained is dry, can arrive pulverous soybean peptides.
2. the method for raising lower molecular weight soybean peptides output according to claim 1, is characterized in that, the enzymolysis solution pol of described step (2) gained is 5～20BX, and pH value is 6～11.
3. the method for raising lower molecular weight soybean peptides output according to claim 1 and 2, is characterized in that, in described step (3), filtrate pol is 4～15BX, and in cleaning process, the weight ratio of water and filtrate is 1:1～1:15.
4. the method for raising lower molecular weight soybean peptides output according to claim 3, is characterized in that, in described step (3), filtrate pol is 15BX, and in cleaning process, the weight ratio of water and filtrate is 1:15.
5. the method for raising lower molecular weight soybean peptides output according to claim 1 and 2, is characterized in that, in described step (4), the pol of concentrated solution is 10～35BX.
6. the method for raising lower molecular weight soybean peptides output according to claim 5, is characterized in that, in described step (4), the pol of concentrated solution is 12BX.
7. according to the method for the raising lower molecular weight soybean peptides output described in claim 3 or 5, it is characterized in that, the enzymolysis time of described step (2) soybean protein isolate solution is 2h～10h.
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| CN201310478523.2A CN103695513B (en) | 2013-10-14 | 2013-10-14 | A kind of method improving yield of soybean peptide with low molecular weight |
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| CN201310478523.2A CN103695513B (en) | 2013-10-14 | 2013-10-14 | A kind of method improving yield of soybean peptide with low molecular weight |
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| CN103695513B CN103695513B (en) | 2016-04-06 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614899A (en) * | 2016-01-13 | 2016-06-01 | 刘新旗 | Low-molecular soybean peptide and preparation method thereof |
| CN107674900A (en) * | 2017-06-12 | 2018-02-09 | 诺利如（安阳）生物科技有限公司 | A kind of process using series connection film concentration Soybean Peptide |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN114480546A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Method for improving yield of low-molecular-weight soybean peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974589A (en) * | 2010-10-29 | 2011-02-16 | 江南大学 | Method for preparing immunocompetent soybean peptide by enzymolysis and membrane separation |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
-
2013
- 2013-10-14 CN CN201310478523.2A patent/CN103695513B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974589A (en) * | 2010-10-29 | 2011-02-16 | 江南大学 | Method for preparing immunocompetent soybean peptide by enzymolysis and membrane separation |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614899A (en) * | 2016-01-13 | 2016-06-01 | 刘新旗 | Low-molecular soybean peptide and preparation method thereof |
| CN105614899B (en) * | 2016-01-13 | 2019-02-01 | 刘新旗 | A kind of low molecule soybean peptide and preparation method thereof |
| CN107674900A (en) * | 2017-06-12 | 2018-02-09 | 诺利如（安阳）生物科技有限公司 | A kind of process using series connection film concentration Soybean Peptide |
| CN107674900B (en) * | 2017-06-12 | 2021-05-14 | 诺利如一（安阳）生物科技有限公司 | Process method for concentrating soybean peptide by adopting tandem membrane |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN114480546A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Method for improving yield of low-molecular-weight soybean peptide |
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| CN103695513B (en) | 2016-04-06 |
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Inventor after: Liu Xinqi Inventor after: Fu Hui Yan Inventor after: Zhang Haiping Inventor after: Guo Ji Inventor before: Yue Bingxiang Inventor before: Fu Hui Yan Inventor before: Zhang Haiping Inventor before: Zhang Xinwei |
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Effective date of registration: 20160531 Address after: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Patentee after: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee after: NUOLI RUYI (BEIJING) BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Patentee before: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. |
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Effective date of registration: 20200827 Address after: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Co-patentee after: RONGHAI BIOTECH Co.,Ltd. Patentee after: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Address before: Tangyin County of Anyang City, the 456150 industrial agglomeration in Henan Province four Street on the south side of the road on the west side of the longitudinal work area Jingjhong Co-patentee before: NUOLI RUYI (BEIJING) BIOLOGICAL TECHNOLOGY Co.,Ltd. Patentee before: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. |
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Address after: 455000 West Road, 100 meters south of the intersection of Jingzhong Road and Xinglong Road in Tangyin County, Anyang City, Henan Province Patentee after: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee after: Shanggu Heyi (Zhenjiang) Food Technology Co.,Ltd. Address before: 456150 west side of gongzongsi street, south side of Jingzhong Road, industrial agglomeration zone, Tangyin County, Anyang City, Henan Province Patentee before: NUOLI RUYI (ANYANG) BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Patentee before: RONGHAI BIOTECH CO.,LTD. |