CN106359839A - Extraction method of oyster peptides - Google Patents
Extraction method of oyster peptides Download PDFInfo
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- CN106359839A CN106359839A CN201610697267.XA CN201610697267A CN106359839A CN 106359839 A CN106359839 A CN 106359839A CN 201610697267 A CN201610697267 A CN 201610697267A CN 106359839 A CN106359839 A CN 106359839A
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- oyster
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
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Abstract
The invention discloses an extraction method of oyster peptides, and belongs to the field of natural medicine extraction. The oyster peptides are prepared through the steps of mixing, extraction, enzyme killing, centrifuge, filter adsorption, separation and drying. A raw material used by the extraction method can select many species, the extraction method has the advantages of no addition of organic solvents, low cost and short cycle, and obtained oyster peptide powder has the advantages of high content of nicotinic acid and selenium, good mouthfeel, no peculiar smell and no bitterness, and can be widely applied to the fields of foods, healthcare products and medicines.
Description
Technical field
The invention belongs to natural drug extracts field, more particularly, to a kind of extracting method of oyster peptide.
Background technology
Concha Ostreae also known as oysters, are a kind of bivalves, are distributed mainly on temperate zone and tropical each ocean stretch of coastal water.
The Shandong Province Concha Ostreae of China is widely distributed and has large-scale artificial cultivation Concha Ostreae.It is rich in abundant Calcium Carbonate, phosphorus in Concha Ostreae
Ten kinds of sour calcium, calcium sulfate, magnesium, aluminum, silicon, ferrum oxide, glycogen, taurine etc. must aminoacid, Glutathione, vitamin a, b1,
B2, d, inanimate matter copper, zinc.Manganese, barium, phosphorus etc., wherein leucine, arginine, citrulline content the abundantest, be so far
One of content highest marine species that the mankind are found.The common eating method of Concha Ostreae is to eat something rare, but breaking due to human body gastric acid
Bad effect, eats something rare and simply obtains little a part of nutrient in Concha Ostreae, most nutrition is not absorbed by the body.
In order to strengthen the absorption to nutritive value in Concha Ostreae, nutritionists have done substantial amounts of research and have found absorption of human body egg
White matter is mainly absorption in the form of peptide, and peptide can adjust each system of human body and the physiological function of cell, and activation has in vivo
Close enzyme element, therefore, peptide becomes the important substance of impact health.Oyster peptide is nutritive value highest and it can be very in Concha Ostreae
The good Concha Ostreae extract being absorbed by the body.Oyster peptide does not contain only the suitably micro unit of rich in protein, vitamin, ratio
Element and taurine, but also contain multiple nutrient substance specific to marine organisms.Natural taurine is obtained, medically in Concha Ostreae
Confirm have blood fat reducing, always platelet aggregation, improve the functions such as hyperglycemic symptoms.Thus twisting in arteriosclerosis, coronary heart disease, the heart
Bitterly, again preferably curative effect in the disease treatment such as hyperlipidemia, arrhythmia, diabetes, chronic hepatitiss.Meanwhile, oyster peptide is in antagonism
Cancer and prevent cancerous cell diffusion in also have certain effect.Additionally, play the role of spermatogenesis to male strong, can cure because of sperm
The very few male sterility causing, can treat climacteric syndrome, pubescent uterus functional bleeding, antenatal to women
Or the symptom such as puerperal asthenia.Main therapeutic domain is that desilting dredging collateral, spermatogenesis reinforce the kidney, liver-invigorating detoxication, set up, grow appearance and support
Face, raising immunity, promotion metabolism etc..The importance of oyster peptide also extensively causes the attention of each scholar expert, oyster peptide
Extracting solution is more paid close attention to.Number of patent application is a kind of 201110449624.8 Chinese patent " extraction side of oyster peptide
Method " in its basic technology of extracting method of introducing be followed successively by steaming and decocting under High Temperature High Pressure, steaming and decocting under constant temperature and pressure, enzymolysis sterilizing, plus
Enter activated carbon deodorization.But the method digestion time and enzymolysis time are long, deodorization, go heavy metal, go fat and other effects inconspicuous.
Content of the invention
It is an object of the invention to provide a kind of extracting method of oyster peptide, this extracting method overcomes lacking of said method
Point, and it makes and can choose that raw material is many, this production technology is without any organic solvent, low cost, cycle is short, gained clam Concha Ostreae
Powder moisture is few, salinity is low, molecular weight is little, dissolubility is strong, biological activity is high, purity is high, in good taste, be easy to store and transport
Defeated.
The technical scheme is that the extracting method of seed oyster peptide one by one, comprise the following steps:
(1) mix: choose fresh Concha Ostreae, remove shell and rub as raw material, add pure water to use food stage after stirring
Sodium hydroxide adjusts ph, is subsequently adding alkaline protease mix homogeneously；
(2) extract: mixture is put into supercritical extractor, adjusts temperature, pressure is extracted；
(3) enzyme denaturing: after extracting, the extracting solution of gained heats up and makes its enzyme denaturing；
(4) it is centrifuged: with centrifuge, extracting solution centrifugation is obtained oyster extract；
(5) Filtration Adsorption: oyster extract is through ion filter Filtration Adsorption；
(6) separate: filtrate is carried out separating, concentrates through nanofiltration separation device；
(7) it is dried: concentrated solution is dried.
Optimally, in described step (1), the addition of pure water is 0.8-1 times of Concha Ostreae minced meat weight, uses food stage hydrogen-oxygen
Changing the ph after sodium is adjusted is 7.6-8.2, and the concentration of alkaline protease is 20u/mg, and addition is the 1 ~ 2% of Concha Ostreae minced meat weight.
Optimally, described alkaline protease is exoproteinase, comprises Collagenase, Digestive Enzyme, glycogen enzyme, nuclease
Deng.
Optimally, in described step (2), the pressure of supercritical extraction is 30-50mpa, and temperature is 45-50 DEG C, extraction time
For 50 ~ 70min.
Optimally, in described step (3), the temperature of enzyme denaturing is 90-95 DEG C, and the time is 10-15min.
Optimally, resin used by described step (5) intermediate ion filter is hydroxyl negative and positive macroporous resin.
Optimally, in described step (6), membrane aperture used by nanofiltration separation device is 1 nanometer.
Optimally, described drying is to be spray-dried.
In the present invention, supercritical extraction can effectively extract required material and during there is cleaning, nontoxic, no dirt
The features such as dye, mild condition, extraction time are short, and supercritical extraction technique can be high boiling point, low volatility, the thing being easily pyrolyzed
Matter extracts so as to can fully combine, thus enzyme can be more preferably faster with enzyme well under far below its boiling temperature
The strand of shearing Concha Ostreae.Supercritical extraction makes extraction and detached step unite two into one, and extraction time is than common enzymatic isolation method
Shorten 33%, and whole process is using organic solvent, the therefore vehicle substance of extract absolutely not residual, thus having prevented
Injury that the presence of machine solvent brings to health and environment is it is ensured that the natural sex of product 100%.In supercritical extraction mistake
In journey, pressure and temperature can become the parameter adjusting extraction process, reach the mesh of extraction by changing temperature and pressure
, and this technique both can adjust temperature and pressure simultaneously, also can fix a parameter and only adjust another one parameter and can protect
Hold temperature-resistant regulation pressure or keep pressure constant regulation temperature to reach the purpose of extraction so that process is simple is easily slapped
Hold and rate of extraction is fast.Intensification enzyme denaturing makes enzyme lose activity so that the purification of follow-up oyster liquid；Centrifugal process is sloughed in extracting solution
Fat and solid content, product reach zero fat.Resin used by ion filter is hydroxyl negative and positive macroporous resin, and it not only can take off
Smelling removal, and energy Adsorption of Heavy Metals well, can make products taste more preferably, and the more preferable quality safety ensureing product, completely
Sufficient food and the demand of health product raw material.Separation process through nanofiltration membrane separation device can slough salt in extracting solution it is dense
Contracting and whole process all at normal temperatures operation, no phase-state change, do not produce pollution, in the present invention the preferred membrane aperture of NF membrane be 1
Nanometer NF membrane, not only can preferably slough molecular weight be less than 100 dalton salts substances, make product quality pure and
Filtrate can be made to concentrate concentration time, the reducing energy consumption saving subsequent handling.The extracting method W-response mild condition of the present invention,
Easy and simple to handle and security performance is high, cycle is short, low cost, do not produce any poisonous and harmful substance, and extraction according to the present invention
In the oyster peptide of method gained, peptide molecular weight reaches more than 95% for the peptide content of 300 ~ 1000 dalton, and molecular weight is not less than
The peptide content of 1000 dalton, within 5%, can be absorbed by the body well, the Concha Ostreae peptide arginine of present invention production simultaneously, melon
Propylhomoserin, leucic content are high, and products taste is good, free from extraneous odour, no bitterness, can be widely applied to food, health product and medicine etc.
Field.
Specific embodiment
The present invention is further illustrated to adopt specific embodiment below.
Embodiment one
Choose fresh Concha Ostreae some, remove shell, rubbed with meat grinder and be weighed as 1000g, add 800ml pure water to stir
Add food sodium hydroxide to adjust ph to 7.6 afterwards, add the alkaline protease 10g of 20u/mg, then put it into super facing
Boundary's extracter, is warming up to 45 DEG C, and pressure is adjusted to 30mpa, extracts 50min.After the completion of extraction, extracting solution is warming up to 90 DEG C, holds
Continuous 10min, makes extracting solution enzyme denaturing.Extracting solution centrifuge is sloughed and sloughs through filtering adsorpting device again after fat and solid content
Fishy smell and heavy metal, the nanofiltration separation device being then 1 nanometer through membrane aperture again carries out separating so as to desalination concentrating.Will
Concentrated solution obtains final product oyster peptide fine work after being spray-dried.Table 1 is the examining report of embodiment 1 products obtained therefrom
Table 1: the examining report of embodiment 1 products obtained therefrom
| Detection project | National Specification (100g) | Testing result |
| Form | Powder, no caking phenomenon | Powder, no lumps |
| Impurity | The no visible exogenous impurity of twenty-twenty vision | Free from admixture |
| Peptide (molecular weight 300-1000 dalton) content | ≥75.0% | 95.8% |
| Ph value (10wt% aqueous solution) | 7.0-8.0 | 7.3 |
| Moisture | ≤7.0% | 3.55% |
| Ash | ≤10.0% | 1.00% |
| Arginine | 8.8 | |
| Leucine | 4.5 | |
| Tryptophan | 0.9 | |
| Arsenic content (in terms of as element) | ≤0.5mg/kg | 0.01mg/kg |
| Lead content (in terms of pb element) | ≤0.5mg/kg | 0.02mg/kg |
| Total number of bacteria | ≤30000cfu/g | 1000cfu/g |
| Coliform | ≤40mpn/100g | ＜ 20mpn/100g |
| Mycete and yeast | ≤50cfu/g | ＜ 20cfu/g |
| Pathogenic bacterium | Must not detect | Do not detect |
Examining report from table 1: the fishy smell of the Concha Ostreae peptide product of this technique gained product as none, as food mouthfeel
Good, molecular weight is little, is almost all small peptide product, and other indexs are also higher than all national standard, particularly arginine, melon ammonia
Sour, leucic comparision contents are high and are practically free of heavy metal.
Embodiment two
Choose fresh Concha Ostreae some, remove shell, rubbed with meat grinder and be weighed as 2000g, add the stirring of 2000ml pure water all
Add food sodium hydroxide to adjust ph to 8.2 after even, add the alkaline protease 40g of 20u/mg, then put it into super
Critical extracter, is warming up to 50 DEG C, and pressure is adjusted to 50mpa, extracts 70min.After the completion of extraction, extracting solution is warming up to 95 DEG C,
Continue 15min, make extracting solution enzyme denaturing.Extracting solution centrifuge is sloughed and takes off through filtering adsorpting device again after fat and solid content
Smelling removal and heavy metal, the nanofiltration separation device being then 1 nanometer through membrane aperture again carries out separating so as to desalination concentrating.
Oyster peptide fine work is obtained final product after concentrated solution is spray-dried.Table 2 is the examining report of embodiment 2 products obtained therefrom
Table 2: the examining report of embodiment 2 products obtained therefrom
| Detection project | National Specification (100g) | Testing result |
| Form | Powder, no caking phenomenon | Powder, no lumps |
| Impurity | The no visible exogenous impurity of twenty-twenty vision | Free from admixture |
| Peptide (molecular weight 300-1000 dalton) content | ≥75.0% | 96.8% |
| Ph value (10wt% aqueous solution) | 7.0-8.0 | 7.3 |
| Moisture | ≤7.0% | 1.55% |
| Ash | ≤10.0% | 0.08% |
| Arginine | 9.8 | |
| Leucine | 5.5 | |
| Tryptophan | 1.3 | |
| Arsenic content (in terms of as element) | ≤0.5mg/kg | 0.01mg/kg |
| Lead content (in terms of pb element) | ≤0.5mg/kg | 0.01mg/kg |
| Total number of bacteria | ≤30000cfu/g | 1000cfu/g |
| Coliform | ≤40mpn/100g | ＜ 10mpn/100g |
| Mycete and yeast | ≤50cfu/g | ＜ 10cfu/g |
| Pathogenic bacterium | Must not detect | Do not detect |
Examining report from table 2: the fishy smell of the Concha Ostreae peptide product of this technique gained product as none, as food mouthfeel
Good, molecular weight is little, is almost all small peptide product, and other indexs are also higher than all national standard, particularly arginine, melon ammonia
Sour, leucic comparision contents are high and are practically free of heavy metal.
Embodiment three
Choose fresh Concha Ostreae some, remove shell, rubbed with meat grinder and be weighed as 100kg, add 900 liters of pure water to stir
Add food sodium hydroxide to adjust ph to 7.9 afterwards, add the alkaline protease 40g of 20u/mg, then put it into super facing
Boundary's extracter, is warming up to 48 DEG C, and pressure is adjusted to 40mpa, extracts 60min.After the completion of extraction, extracting solution is warming up to 93 DEG C, holds
Continuous 13min, makes extracting solution enzyme denaturing.Extracting solution centrifuge is sloughed and sloughs through filtering adsorpting device again after fat and solid content
Fishy smell and heavy metal, the nanofiltration separation device being then 1 nanometer through membrane aperture again carries out separating so as to desalination concentrating.Will
Concentrated solution obtains final product oyster peptide fine work after being spray-dried.Table 3 is the examining report of embodiment 3 products obtained therefrom
Table 3: the examining report of embodiment 3 products obtained therefrom
| Detection project | National Specification (100g) | Testing result |
| Form | Powder, no caking phenomenon | Powder, no lumps |
| Impurity | The no visible exogenous impurity of twenty-twenty vision | Free from admixture |
| Peptide (molecular weight 300-1000 dalton) content | ≥75.0% | 97.8% |
| Ph value (10wt% aqueous solution) | 7.0-8.0 | 7.6 |
| Moisture | ≤7.0% | 1.15% |
| Ash | ≤10.0% | 0.07% |
| Arginine | 9.9 | |
| Leucine | 5.8 | |
| Tryptophan | 1.5 | |
| Arsenic content (in terms of as element) | ≤0.5mg/kg | 0.01mg/kg |
| Lead content (in terms of pb element) | ≤0.5mg/kg | 0.01mg/kg |
| Total number of bacteria | ≤30000cfu/g | 1000cfu/g |
| Coliform | ≤40mpn/100g | ＜ 10mpn/100g |
| Mycete and yeast | ≤50cfu/g | ＜ 10cfu/g |
| Pathogenic bacterium | Must not detect | Do not detect |
Examining report from table 3: the fishy smell of the Concha Ostreae peptide product of this technique gained product as none, as food mouthfeel
Good, molecular weight is little, is almost all small peptide product, and other indexs are also higher than all national standard, particularly arginine, melon ammonia
Sour, leucic comparision contents are high and are practically free of heavy metal.
The above, be only to presently preferred embodiments of the present invention, is not the restriction that the present invention is done with other forms,
Any those skilled in the art are changed or are modified as equivalent variations possibly also with the technology contents of the disclosure above
Equivalent embodiments.But, every without departing from the present invention program content, the technical spirit according to the present invention is done to above example
Any simple modification, equivalent variations and remodeling, still fall within protection scope of the present invention.
Claims (8)
1. a kind of extracting method of oyster peptide is it is characterised in that comprise the following steps:
(1) mix: choose fresh Concha Ostreae, remove shell and rub as raw material, add pure water to use food stage after stirring
Sodium hydroxide adjusts ph, is subsequently adding alkaline protease mix homogeneously；
(2) extract: mixture is put into supercritical extractor, adjusts temperature, pressure is extracted；
(3) enzyme denaturing: after extracting, the extracting solution of gained heats up and makes its enzyme denaturing；
(4) it is centrifuged: with centrifuge, extracting solution centrifugation is obtained oyster extract；
(5) Filtration Adsorption: oyster extract is through ion filter Filtration Adsorption；
(6) separate: filtrate is carried out separating, concentrates through nanofiltration separation device；
(7) it is dried: concentrated solution is dried.
2. the extracting method of oyster peptide according to claim 1 is it is characterised in that the addition of described pure water is that Concha Ostreae is broken
0.8-1 times of meat, the ph after being adjusted with food sodium hydroxide is between 7.6-8.2, and the concentration of alkaline protease is 20u/mg,
Addition is the 1-2% of Concha Ostreae minced meat weight.
3. the extracting method of oyster peptide according to claim 1 and 2 is it is characterised in that described alkaline protease is circumscribed
Protease, comprises Collagenase, Digestive Enzyme, glycogen enzyme, nuclease etc..
4. the extracting method of oyster peptide according to claim 1 is it is characterised in that the pressure of described supercritical extraction is
30-35mpa, temperature is 45-50 DEG C, and extraction time is 50-70min.
5. oyster peptide according to claim 1 extracting method it is characterised in that described enzyme denaturing temperature be 90-95 DEG C,
Time is 10-15min.
6. the extracting method of oyster peptide according to claim 1 is it is characterised in that resin used by described ion filter is
Hydroxyl negative and positive macroporous resin.
7. the extracting method of oyster peptide according to claim 1 is it is characterised in that fenestra used by described nanofiltration separation device
Footpath is 1 nanometer.
8. the extracting method of oyster peptide according to claim 1 is it is characterised in that described drying is to be spray-dried.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106176874A (en) * | 2016-08-22 | 2016-12-07 | 得利斯集团有限公司 | A kind of preparation method of Maca extract |
| CN107034260A (en) * | 2017-06-27 | 2017-08-11 | 钦州学院 | A kind of preparation method of Nano-Zinc ostreae testa pulverata |
| CN107997184A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | The preparation method of one seed oyster oligopeptide product |
| CN107998369A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen |
| CN109007857A (en) * | 2018-08-29 | 2018-12-18 | 苏州佩普肽康生物科技有限公司 | Improve the food formula of special diet and preparation method thereof of mankind spermatozoon quality |
| CN113854473A (en) * | 2021-09-30 | 2021-12-31 | 无锡定象改性硅胶材料有限公司 | Method for preparing low-arsenic oyster protein peptide by adsorption of targeting nano silica gel material |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN107034260A (en) * | 2017-06-27 | 2017-08-11 | 钦州学院 | A kind of preparation method of Nano-Zinc ostreae testa pulverata |
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| CN107997184A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | The preparation method of one seed oyster oligopeptide product |
| CN107998369A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen |
| CN109007857A (en) * | 2018-08-29 | 2018-12-18 | 苏州佩普肽康生物科技有限公司 | Improve the food formula of special diet and preparation method thereof of mankind spermatozoon quality |
| CN113854473A (en) * | 2021-09-30 | 2021-12-31 | 无锡定象改性硅胶材料有限公司 | Method for preparing low-arsenic oyster protein peptide by adsorption of targeting nano silica gel material |
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Application publication date: 20170201 |