CN106176874A - A kind of preparation method of Maca extract - Google Patents
A kind of preparation method of Maca extract Download PDFInfo
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- CN106176874A CN106176874A CN201610697775.8A CN201610697775A CN106176874A CN 106176874 A CN106176874 A CN 106176874A CN 201610697775 A CN201610697775 A CN 201610697775A CN 106176874 A CN106176874 A CN 106176874A
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Abstract
The invention discloses the preparation method of a kind of Maca extract, belong to natural medicine field, the Maca extract of the present invention by mixing, ultrasonic extraction, enzyme denaturing, filtration, Filtration Adsorption, concentrate prepared with the step being dried；The preparation method of the present invention produces without any organic solvent, low cost, cycle short, and the alcohol-free abnormal flavour of products obtained therefrom, dissolubility is strong, biological activity is high, purity is high, be easily absorbed by the human body, be easy to store and transport, and scale industrialization is feasible.
Description
Technical field
The invention belongs to natural medicine field, relate to the extraction of a kind of natural drug, particularly relate to a kind of Maca extract
Preparation method.
Background technology
Lepidinm meyenii Walp (Maco) has another name called Maca, for Cruciferae separate row Vegetable spp annual herb plant, is grown on Peru's height above sea level
The mountain area, Andean of more than 3500m, can be without fertilizer, anoxia, day and night temperature greatly, normally give birth under the most ice-bound unique environments
Long.Lepidinm meyenii Walp rich in nutritive value, enjoys the good reputation such as " Peru's Radix Ginseng ", " plant viagra ".Lepidinm meyenii Walp rich in 50 multiple nutritional components, its
Middle vegetable protein, aminoacid, polysaccharide, unsaturated fatty acid, mineral potassium, magnesium, zinc equal size are abundant, additionally, it is possibly together with mustard
The important biomolecule active substances such as seed oil glycosides, alkaloid, plant sterol, Maca alkene and macamide so that it is produce the highest medicinal
It is worth.Lepidinm meyenii Walp have resisting fatigue, improve immune, anticancer, balance hormone, endocrine regulation, improve sexual function, treatment climacteric
The multi-efficiencies such as syndrome, promotion fertility, are especially considered as Andean in the function strengthening the aspect such as energy and fertility by people
The rich gift that mountain god vouchsafes.
Lepidinm meyenii Walp is pointed out that a kind of nutritious safe food very early, can solve multiple to cause because of undernutrition
Health problem, but the most attention that Lepidinm meyenii Walp obtains International Plant scholar and pharmaceutical researchers is from the eighties in 20th century,
After particularly the mid-90 Italian Dini A etc. has delivered the component analysis of root dry to Lepidinm meyenii Walp, along with the modern times divide
Analysis and pharmacological effect technology are increasingly mature, and the chemical analysis of Lepidinm meyenii Walp, effect, toxicity and clinical research are in late 1990s
There is large development.Alkaloids along with macamide, Lepidinm meyenii Walp alkene and some structures uniqueness etc. are by the analysis of system, to Lepidinm meyenii Walp
The most constantly going deep at the efficacy study and clinical research improving the aspect such as fertility, sexual desire promoting, Lepidinm meyenii Walp is gradually by tradition
Food has turned to health food.Owing to getting more and more the demand of Lepidinm meyenii Walp, sight has been placed on effectively by increasing people
Prepare on the extract of Lepidinm meyenii Walp.Number of patent application is the patent application " preparation of a kind of Maca extract of 201410532870.3
Method " in disclose the preparation technology of a kind of Lepidinm meyenii Walp, its step is main the most successively: (1) Lepidinm meyenii Walp tuber is cut into slices；(2) pulverize, ultrasonic carry
Take；(3) release upper strata and extract solution, supersound extraction；(4) release upper strata and extract solution, supersound extraction；(5) expression separation, obtains
Layer extracts solution and extracts residue；(6) merging the upper strata extraction solution of (3), (4) and (5), centrifugation obtains solid content 1. and clearly
Liquid is 2.；(7) 2. clear liquid is concentrated in vacuo to extract concentrate 3.；(8) solid content 1. solid-liquid separation obtains extracting solution and solid content；(9)
Solid content solid-liquid separation obtains extracting solution and solid content；(10) merge (8) and the extracting solution of (9), be concentrated in vacuo to extract concentrate
④；(11) 5. the extraction residue supersound extraction of (5) must be extracted concentrate；(12) (7), (10) and the concentrate of (11) are merged,
Obtain Maca extract.Its reactions steps is loaded down with trivial details, the longest, is not suitable for large-scale industrial production and uses big during it extracts
The ethanol solution of amount, affects quality and the mouthfeel of product.
Summary of the invention
In order to overcome drawbacks described above, the present invention provides the preparation method of a kind of new Maca extract, and this preparation method is raw
It is short to produce low cost, cycle, and the alcohol-free abnormal flavour of products obtained therefrom, dissolubility is strong, biological activity is high, purity is high, be easily absorbed by the human body, just
In storing and transport, scale industrialization is feasible.
The technical scheme is that the preparation method of a kind of Maca extract, mainly comprise the steps of,
(1) mixing: choose high-quality Lepidinm meyenii Walp, as raw material after pulverizing, adds and adjusts with food stage sodium hydroxide after pure water stirs
Joint PH, is subsequently adding compound protease mix homogeneously；
(2) ultrasonic extraction: mixture is put into ultrasonic extractor and extracts；
(3) enzyme denaturing: after extracting, the extracting solution of gained heats up and makes its enzyme denaturing；
(4) filter: enzymolysis solution filters through microporous filter, then takes its clear liquid；
(5) Filtration Adsorption: clear liquid is through ion filter Filtration Adsorption；
(6) concentrate；
(7) it is dried.
Preferably, in described step (1), the addition of pure water is 4-5 times of raw material weight, adjusts with food stage sodium hydroxide
PH after joint is 7.6-8.2, and the concentration of compound protease is 25U/mg, and addition is the 1-2% of Lepidinm meyenii Walp weight.
Preferably, in described step (1), compound protease comprises alkaline protease, amylase, peptide ending enzyme, glycogen enzyme, core
Acid enzyme etc..
Preferably, in described step (2), the power of ultrasonic extraction is 230-250 watt, and temperature is 50-55 DEG C, and the time is
70-80min。
Preferably, in described step (3), the temperature of enzyme denaturing is 90-95 DEG C, and the time is 10-15min.
Preferably, in described step (6), concentrate and concentrate into dual-effect concentrator.
Preferably, in described step (7), it is dried as being spray-dried.
In the present invention, Maca extract uses ultrasound wave enzymolysis and extraction, and Lepidinm meyenii Walp is after being ultrasonically treated, and cell membrane is
Broken, this make compound protease can fully and intracellular protein contacts thus the strand of fast shut-off protein,
The molecular weight making product is less, and the used time short biological activity that more can preferably keep product.The method using ultrasonic extraction
Extracting temperature is low, yield is high and the time used is short.The choosing of the most several protease makes the various protease can phase interworking especially
Close, accelerate enzymolysis speed so that vehicle is more easy to filter, thus saves the time of the step such as subsequent filter, Filtration Adsorption, simultaneously
Reduce energy consumption.Wherein peptide ending enzyme reduces the bitterness of Maca extract especially so that products taste is more preferable.Microporous filter will extract
Liquid obtains the materials such as large particulate matter such as cell wall residue remove, then slough its contained huge sum of money through ion filter
Belong to so that product is safer.On the whole, the preparation method of the Maca extract provided in the present invention is with short production cycle, reaction
Mild condition, Extracting temperature are low.Extract product yield height, low cost, process safety and do not produce any poisonous and harmful substance together
Time be suitable to large-scale production；Product purity high molecular prepared by this extracting method is little, and wherein 300 ~ 1000 daltonian peptides divide
Son amount accounts for more than the 95% of peptide content, and molecular weight more than 1000 daltonian peptide molecular weights within 5%, the human body suction to it
Yield reaches 100% especially.Without ethanol in the preparation process of the what is more important present invention, product safety and reliability.This system
The product that Preparation Method is produced can be widely applied to the fields such as food, health product and medicine.
Detailed description of the invention
The present invention is further illustrated to use detailed description of the invention below.
Embodiment one
Choose high-quality dry Maca 1000g, pulverize as raw material with pulverizer, add 4000ml purified water and stir；With food
Grade sodium hydroxide regulation pH to 7.6, is subsequently adding the compound protease of the 25U/mg of 10 grams；Dispensing is proceeded to ultrasound wave carry
The machine of taking extracts, and Extracting temperature is maintained at 50 DEG C, and ultrasonic power is 230 watts, and extraction time is 70min, during extraction
PH value keeps stable；After having extracted, the extracting solution obtained is warming up to 90 DEG C, keeps 10min to make its enzyme denaturing；By enzymolysis solution with micro-
Hole filter filters, and then takes cleaner liquid through filtrate through ion filter Filtration Adsorption so that it is slough heavy metal；Then will filter
Liquid dual-effect concentrator concentrates；Last concentrated solution i.e. obtains Maca extract fine work after being spray-dried.
Embodiment two
Choose high-quality dry Maca 5kg, pulverize as raw material with pulverizer, add 25l purified water and stir；Use food stage hydrogen
Sodium oxide regulation pH to 8.2, is subsequently adding the compound protease of the 25U/mg of 100 grams；Dispensing is proceeded to ultrasonic extractor enter
Row extracts, and Extracting temperature is maintained at 55 DEG C, and ultrasonic power is 250 watts, and extraction time is 80min, and during extraction, pH value is protected
Keep steady fixed；After having extracted, the extracting solution obtained is warming up to 95 DEG C, keeps 15min to make its enzyme denaturing；By enzymolysis solution microporous filter
Machine filters, and then takes cleaner liquid through filtrate through ion filter Filtration Adsorption so that it is slough heavy metal；Then by filtrate with double
Effect concentrator concentrates；Last concentrated solution i.e. obtains Maca extract fine work after being spray-dried.
Embodiment three
Choose high-quality dry Maca 100kg, pulverize as raw material with pulverizer, add 450l purified water and stir；Use food
Sodium hydroxide regulation pH to 7.9, is subsequently adding the compound protease of the 25U/mg of 1.5kg；Dispensing is proceeded to ultrasonic extraction
Machine extracts, and Extracting temperature is maintained at 53 DEG C, and ultrasonic power is 240 watts, and extraction time is 75min, pH during extraction
Value keeps stable；After having extracted, the extracting solution obtained is warming up to 92 DEG C, keeps 13min to make its enzyme denaturing；By enzymolysis solution micropore
Filter filters, and then takes cleaner liquid through filtrate through ion filter Filtration Adsorption so that it is slough heavy metal；Then by filtrate
Concentrate with dual-effect concentrator；Last concentrated solution i.e. obtains Maca extract fine work after being spray-dried.
Claims (7)
1. the preparation method of a Maca extract, it is characterised in that mainly comprise the steps of,
(1) mixing: choose high-quality Lepidinm meyenii Walp, as raw material after pulverizing, adds and adjusts with food stage sodium hydroxide after pure water stirs
Joint PH, is subsequently adding compound protease mix homogeneously；
(2) ultrasonic extraction: mixture is put into ultrasonic extractor and extracts；
(3) enzyme denaturing: after extracting, the extracting solution of gained heats up and makes its enzyme denaturing；
(4) filter: enzymolysis solution filters through microporous filter, then takes its clear liquid；
(5) Filtration Adsorption: clear liquid is through ion filter Filtration Adsorption；
(6) concentrate；
(7) it is dried.
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (1), pure water
4-5 times that addition is raw material weight, the PH after regulating with food stage sodium hydroxide is 7.6-8.2, compound protease dense
Degree is 25U/mg, and addition is the 1-2% of Lepidinm meyenii Walp weight.
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (1), compound
Protease comprises alkaline protease, amylase, peptide ending enzyme, glycogen enzyme, nuclease etc..
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (2), ultrasonic
The power that ripple extracts is 230-250 watt, and temperature is 50-55 DEG C, and the time is 70-80min.
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (3), enzyme denaturing
Temperature be 90-95 DEG C, the time is 10-15min.
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (6), concentrates
Concentrate for dual-effect concentrator.
The preparation method of a kind of Maca extract the most as claimed in claim 1, it is characterised in that in described step (7), is dried
For being spray-dried.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106913604A (en) * | 2017-01-19 | 2017-07-04 | 大连豪翔生物酶工程有限公司 | A kind of integrated extraction process of agate card active component |
| CN109453300A (en) * | 2018-12-29 | 2019-03-12 | 雨润慕德生物科技（连云港）有限公司 | A kind of extracting method and its application of macamide and glucosinolate |
| CN112826086A (en) * | 2021-01-06 | 2021-05-25 | 湖北瑞邦生物科技有限公司 | Maca extract and extraction method thereof |
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| CN106261972A (en) * | 2016-08-22 | 2017-01-04 | 得利斯集团有限公司 | A kind of extracting method of Carnis Mactrae peptide |
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| CN102836196A (en) * | 2012-09-29 | 2012-12-26 | 楚雄州百草岭药业发展有限公司 | Maca extract and preparation method thereof |
| CN103816200A (en) * | 2013-09-05 | 2014-05-28 | 李玉山 | Preparation process of maca extractives |
| CN103864949A (en) * | 2014-03-20 | 2014-06-18 | 北京中科健宇生物科技有限公司 | Method for preparing maca polysaccharide and maca alkaloid by use of membrane separation technique |
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| CN106913604A (en) * | 2017-01-19 | 2017-07-04 | 大连豪翔生物酶工程有限公司 | A kind of integrated extraction process of agate card active component |
| CN109453300A (en) * | 2018-12-29 | 2019-03-12 | 雨润慕德生物科技（连云港）有限公司 | A kind of extracting method and its application of macamide and glucosinolate |
| CN109453300B (en) * | 2018-12-29 | 2021-05-14 | 雨润慕德生物科技（连云港）有限公司 | Extraction method and application of macamide and glucosinolate |
| CN112826086A (en) * | 2021-01-06 | 2021-05-25 | 湖北瑞邦生物科技有限公司 | Maca extract and extraction method thereof |
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Application publication date: 20161207 |